Proteomic LC-MS/MS characterization of a protein-enriched S. commune fraction reveals 64 protein groups and potent selective activity against HeLa cancer cells.

Multi-omics profiling of thymoma identifies subtype-specific metabolic, lipid, and protein changes and reveals molecular targets associated with myasthenia gravis.

A derivatization-assisted chiral LC-MS/MS method separates and quantifies carnitine and acetylcarnitine enantiomers in meat, milk, and fermented foods.

An analytical AEX workflow with orthogonal characterization reveals distinct pH-dependent degradation pathways of rAAV8 during accelerated storage.

A systematic UHPLC-MS/MS study shows that matrix-effect estimates based only on calibration slopes can be biased and introduces an intercept-based correction.

A micro-flow LC-Q-Orbitrap HRMS method quantifies 239 pesticides in fruits and vegetables while cutting solvent consumption and waste by 25-fold.

Propionic acid increases peptide identifications by 39% versus formic acid in LC-MS proteomics while maintaining chromatographic performance and compatibility.

A GC-MS/MS method quantifies inorganic fluoride in EOF extracts and shows that fluoride contamination can substantially overestimate organic fluorine measured by CIC.

A two-stage liquid–liquid extraction method enables sensitive, reproducible analysis of ultrashort PFCAs such as TFA and longer-chain PFAS in water.

This review explores lesser-known Czech contributions to chromatography and electrophoresis and highlights recent advances in separation science and instrumentation.