Post-translational modifications (PTMs) are where so much of biology’s complexity lives, but they are also some of the hardest molecular features to analyze with confidence. Phosphorylation, asymmetric dimethylarginine (ADMA), and O-GlcNAcylation (O-GlcNAc) often occur at low abundance, can be highly labile, and frequently require precise site localization to understand their biological meaning. This<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_004377_ov_phosphorylation_ptm_etd_excedion_pro_tn004377_na_en_71f681655f.pdf"> technical note </a>shows how the <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1958">Thermo Scientific Orbitrap Excedion Pro Hybrid Mass Spectrometer</a> helps address those challenges by combining fast, sensitive performance with complementary <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7217">HCD and EThcD fragmentation</a> workflows.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Phosphorylation_ADMA_and_O_Glc_N_Ac_Analysis_on_the_Orbitrap_Excedion_Pro_Mass_Spectrometer_d24a5de98e.jpg" alt="Thermo Fisher Scientific: Phosphorylation, ADMA, and O-GlcNAc Analysis on the Orbitrap Excedion Pro Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Phosphorylation_ADMA_and_O_Glc_N_Ac_Analysis_on_the_Orbitrap_Excedion_Pro_Mass_Spectrometer_d24a5de98e.jpg 245w," sizes="100vw" width="1024" height="512">Using enriched PTM samples and automated data-dependent acquisition, the study demonstrates that the Orbitrap Excedion Pro MS can deliver both depth and confidence across multiple PTM classes. For phosphorylation analysis, the platform identified 5,892 unique phosphosites from IMAC-enriched samples and 2,941 unique phosphotyrosine sites after phosphotyrosine enrichment, highlighting strong performance for both broad and highly targeted signaling studies. Just as importantly, the data show that HCD and EThcD provide complementary value, with each fragmentation method contributing unique site identifications and helping resolve ambiguous assignments.The note also highlights the value of EThcD for more analytically demanding PTMs. In ADMA-enriched samples, EThcD delivered more ADMA peptide-spectrum matches and more unique modified peptides than HCD, supporting improved confidence in site characterization. For O-glycopeptides, the Orbitrap Excedion Pro MS uses an intelligent HCD-product-dependent-EThcD strategy that triggers EThcD only when oxonium ions are detected, improving productivity while preserving the fragmentation detail needed for confident glycosite localization.Overall, this technical note shows how Orbitrap Excedion Pro MS enables researchers to go deeper into PTM biology with greater sensitivity, better site confidence, and smarter acquisition. For scientists studying signaling, regulation, and disease mechanisms, it presents a powerful approach for expanding PTM coverage without compromising analytical rigor.<blockquote><h5><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_004377_ov_phosphorylation_ptm_etd_excedion_pro_tn004377_na_en_71f681655f.pdf">Download the technical note</a></h5></blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#PTM #OrbitrapExcedionPro #glycosylation #phosphorylation

Phosphorylation, ADMA, and O-GlcNAc Analysis on the Orbitrap Excedion Pro Mass Spectrometer

The countdown is almost over. In just one week, the international chromatography community will meet in Prague for the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/events/644">35th International Symposium on Chromatography (ISC 2026)</a>, taking place from September 6–10, 2026. Whether your registration has been confirmed for months or you are still deciding whether to join, now is the time to finalize your plans. Here is what you need to know before ISC 2026 begins.<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/companies/314">ISC</a> is the oldest conference series dedicated to separation science, with symposia organized since 1956. This September, the Prague Congress Centre will welcome researchers, industry experts, instrument manufacturers, young scientists, and other specialists for five days of chromatography and separation science, covering everything from fundamental research to pharmaceutical, environmental, food, health, and omics applications.But before the scientific sessions begin, there are a few practical details worth checking.<h4>ISC officially starts on September 6 – but activities begin a day earlier</h4>The official dates for ISC 2026 are Sunday, September 6 through Thursday, September 10, but for some participants the conference starts already on Saturday, September 5.The first Short Courses are scheduled for Saturday (<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/6770">SC 1 – Modern HPLC Separation in Theory and Practice</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7312">SC 2 – mzmine for LC-MS data analysis</a>), with additional courses continuing on Sunday morning and afternoon. If you have registered for a Short Course, make sure to check its exact date and starting time before arranging your journey to Prague.Sunday then marks the official opening of ISC 2026, with the Opening Ceremony, Award Ceremony, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7361">plenary lectures</a>, and Welcome Reception introducing delegates to the week ahead.<h4>Haven’t registered yet? You can still join ISC 2026</h4>Missed the late-registration deadline? It is still possible to attend.Onsite registration has been available since August 26, 2026, giving last-minute participants another opportunity to join the symposium. Current onsite registration fees are:<ul><li>Industry: €1,150</li><li>Academic: €890</li><li>Student: €390</li><li>Short Courses: €270</li></ul>The Short Course fee covers participation in up to three Short Courses.One practical point for last-minute registration: bank-transfer payments closed on August 19, so only card payments are now accepted.Full registration includes access to oral and poster sessions, the exhibition, the Sunday Welcome Reception, scheduled lunches and coffee breaks, the electronic Book of Abstracts, and a Prague public transportation pass.<h4>Where is ISC 2026 and how do you get there?</h4>ISC 2026 will take place at the:Prague Congress Centre<ul><li>5. května 65</li><li>140 21 Prague 4</li><li>Czech Republic</li></ul>The venue is particularly easy to reach by public transport. It is located directly on Prague’s Metro Line C at Vyšehrad station, just around five minutes by metro from Muzeum station in the city centre.The Prague Congress Centre brings the lecture halls, exhibition spaces, meeting rooms, and other conference facilities together in one venue, making it easy to move between scientific sessions, posters, industry activities, and the exhibition throughout the day.And since full conference registration includes public transportation in Prague, getting around the city during ISC should be straightforward.<h4>When can you pick up your conference materials?</h4>The registration desk will already be open during the Short Courses and will remain available throughout the symposium.Registration opening hours:<ul><li>Saturday, September 5: 8:30–17:00</li><li>Sunday, September 6: 7:30–19:00</li><li>Monday, September 7: 7:30–18:00</li><li>Tuesday, September 8: 7:30–19:00</li><li>Wednesday, September 9: 8:00–18:00</li><li>Thursday, September 10: 8:30–14:30</li></ul>If you are arriving in Prague on Sunday, allowing some extra time for registration before the Opening Ceremony will make the start of the conference considerably more relaxed.<h4>Now is the time to build your personal ISC program</h4>With several parallel sessions running throughout the week, trying to decide where to go only after arriving at the venue may mean missing something important.The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7477">main scientific program</a> is organized around three broad areas:<ul><li>Fundamentals</li><li>Bioanalysis and omics applications</li><li>Hyphenated techniques</li></ul>These are complemented by <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7280">Tutorials </a>+ Young Speakers, plenary and invited lectures, poster sessions, the international exhibition, vendor seminars, and industry-focused activities.Before leaving for Prague, take another look at the Interactive Program and mark the lectures, posters, and other activities that are most relevant to you. If you are presenting your own work, this is also the right time to double-check your assigned session, room, and presentation time.And don’t focus only on the oral program. Poster sessions and exhibition breaks are among the best opportunities to discuss new research directly with authors, meet instrument and technology specialists, and make new contacts.<h4>Don’t overlook the vendor seminars and industry program</h4>ISC 2026 is not limited to academic presentations. The conference also includes an extensive industry component, with activities covering current challenges in separation science and practical applications of new analytical technologies.During the lunchtime sessions, participants can attend <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7736">vendor seminars</a> presented by companies including Avantor, Shimadzu, Advanced Materials Technology, Restek, and Thermo Fisher Scientific. Topics range from stationary-phase selectivity and chromatographic modelling to HPLC and LC-MS column technologies and advanced characterization of biotherapeutics.The conference also includes dedicated <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7777">Industry Activities</a> from Monday through Wednesday, bringing together academic and industrial perspectives on topics relevant to analytical laboratories and the future of separation science. One of the discussions, for example, will examine how education in separation science can better reflect the skills required by modern industry, while another will focus on sustainability and the practical implementation of greener analytical workflows.On Wednesday, participants can also join the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7801">Publishing Workshop</a>, another activity worth adding to the schedule if scientific publishing is part of your work.<h4>Test your chromatography knowledge at the ISC Quiz</h4>Not every session needs to be completely serious.On Tuesday, September 8 from 18:30 to 19:30, ISC will host an interactive Chromatography Quiz, combining practical questions, chromatography fundamentals, unusual facts, and lighter challenges.There is no advance registration required. Any registered conference participant can join, but remember to bring a smartphone—the quiz uses live participation through a QR code.Results will be scored in real time, and prizes will be awarded to the top three participants.<h4>Start networking from the first evening</h4>The scientific program may be the main reason to attend ISC, but the conversations between sessions can be equally valuable.The first major networking opportunity comes immediately on Sunday.The Welcome Reception will take place on September 6 from 18:30 to 20:00 in the second-floor foyer of the Prague Congress Centre. It is open to registered participants and accompanying persons and includes a light buffet and drinks.As it takes place immediately after the opening program, it is an ideal opportunity to reconnect with colleagues, meet new contacts, and start conversations before the full parallel scientific program begins on Monday.Another social highlight will be the Gala Dinner on Wednesday, September 9 from 19:00 to midnight at the National House Vinohrady.The Gala Dinner requires a separate €100 ticket, is not included in the standard registration fee, and has a smart-casual dress code. Participants should also note that transportation to and from the venue is not organized by the conference.<h4>And perhaps leave a little time for Prague</h4>A conference schedule can fill up quickly, but ISC 2026 is taking place in one of Europe’s most walkable cities.If you find some free time between sessions, Vyšehrad is only about a 10-minute walk from the Prague Congress Centre and offers panoramic views across Prague and the Vltava River. It is an easy option for a short walk without travelling back into the historic city centre.See you at ISC 2026 in Prague!

ISC 2026 Starts in One Week: What You Need to Know

In the week of August 31, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 5 500 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Technology Networks: Modern Methods for Faster Microbial Analysis</h5><ul><li>Tu, 1.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6454">Registration</a></li></ul>Struggling to choose the right technique for microbial testing? Learn how MALDI-TOF, FTIR, and automated DNA/RNA electrophoresis accelerate identification.<h5>2. Phenomenex: Addressing LC Method Optimization - Scaling For Efficiency, Throughput And Reproducibility</h5><ul><li>We, 2.9.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6458">Registration</a></li></ul>Join this webinar on LC method optimization and scaling. Learn how column dimensions, particle morphology, and sample loading strategies maximize throughput and reproducibility.<h5>3. Thermo Fisher Scientific: Proteoform Studio software, top down made simple</h5><ul><li>We, 2.9.2026 14:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6459">Registration</a></li></ul>Learn how Thermo Scientific Proteoform Studio software simplifies top-down MS and intact protein analysis through integrated workflows, interactive deconvolution, and reporting.<h5>4. Agilent Technologies: Targeted Hybridization Capture for Antimicrobial Resistance Surveillance in Complex Environmental Samples</h5><ul><li>We, 2.9.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6455">Registration</a></li></ul>Discover how Agilent SureSelect hybrid capture enhances antimicrobial resistance gene detection and characterization in complex environmental samples compared with shotgun NGS.<h5>5. Thermo Fisher Scientific: Ask the Expert: What to expect from the new Thermo Scientific™ Orbitrap™ Excedion™ Hybrid mass spectrometers</h5><ul><li>Th, 3.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6466">Registration</a></li></ul>Discover the new Orbitrap Excedion Hybrid Mass Spectrometer and learn how enhanced dynamic range, robust performance, and an upgradeable design support advanced HRAM workflows.<h5>6. Waters Corporation: Informatics series #34 Ready for Self-Documenting Chromatography?</h5><ul><li>Th, 3.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6426">Registration</a></li></ul>Speed up root cause analysis with self-documenting LC and Empower software in this 30-minute Waters coffee break.<h5>7. SciY/Mestrelab Research: From Legacy Workflows to Paperless Labs: Track Every Sample, Process, and Decision</h5><ul><li>Th, 3.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6425">Registration</a></li></ul>Transform your lab from paper to digital. Learn how WebLab and LIMS workflows automate sample tracking and support laboratory compliance.<h5>8. SelectScience: ADC characterization in one native LC-MS workflow</h5><ul><li>Fr, 4.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6460">Registration</a></li></ul>Simplify ADC characterization with native RP-LC-MS using Biozen® columns to determine DAR, proteoforms, and positional isomers without buffer exchange.

Webinars LabRulezLCMS Week 36/2026

Article

Product

Events

Webinars

Application

Video

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Bioanalytic Individual LC-MS/MS training

We are ready to prepare customized LC-MS/MS training for the needs of participants.

<h4>BASIC INFORMATION</h4><ul><li>TITLE: Customized LC-MS/MS Training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL or other</li><li>TRAINING TIME: 1-5 days or more</li><li>NUMBER OF PARTICIPANTS: 4-8 people</li><li>EQUIPMENT: SCIEX QTRAP/QTOF LC-MS/MS</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg" alt="Bioanalytic: Bioanalytic Individual LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Individual_LC_MS_MS_training_8a73408c56.jpg 245w," sizes="100vw" width="1536" height="930"><h4>TRAINING DESCRIPTION</h4>Customized LC-MS/MS training we are ready to prepare for the needs of participants, discussing:<ul><li>theoretical basics of chromatography, mass spectrometry,</li><li>practical operation of the LC-MS/MS system,</li><li>qualitative and quantitative analysis,</li><li>DDA &amp; DIA screening methods,</li><li>“omics” analyses,</li><li>validation of analytical methods,</li><li>solving basic methodological and technical problems,</li><li>software,</li><li>calibration,</li><li>“tips&amp;tricks”,</li><li>etc...</li></ul>We offer training for both novices starting the fascinating adventure of LC-MS/MS analytics, as well as for those who are LC-MS/MS users and are interested in expanding and consolidating their knowledge.<h4>Framework training program</h4><ul><li>individual LC-MS/MS training is developed according to the customer's demand</li><li>conducting theoretical and/or practical training at the customer's workplace</li><li>carrying out theoretical and/or practical training in a laboratory in Lodz</li></ul>

Bioanalytic Basic LC-MS/MS training

Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument.

<h4>BASIC INFORMATION</h4><ul><li>TITLE: Basic LC-MS/MS training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL</li><li>TRAINING TIME: 3 days</li><li>NUMBER OF PARTICIPANTS: up to 5 people</li><li>EQUIPMENT: SCIEX QTRAP &amp; QTOF LC-MS/MS</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg" alt="Bioanalytic: Bioanalytic Basic LC-MS-MS training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Basic_LC_MS_MS_training_fb1382a55b.jpg 205w," sizes="100vw" width="1293" height="987"><h4>TRAINING DESCRIPTION</h4>Basic LC-MS/MS training covers the theoretical basics of mass spectrometry and familiarization with the basic operation of the instrument. Basic LC-MS/MS training is primarily aimed at those who are new to LC-MS/MS equipment or who wish to become familiar with this analytical technique. During the training, the following will be presented:<ul><li>principles of developing and optimizing quantitative and qualitative methods on low (triple quadrupole, QTRAP) and high resolution (QTOF) instruments,</li><li>basic functionality of the software,</li><li>procedures and objectives of instrument calibration,</li><li>principles of maintaining the cleanliness of mass spectrometer operation.</li></ul><h5>Basic LC-MS/MS training - the program</h5>Day ITheoretical classes:<ul><li>Introduction to LC</li><li>Introduction to mass spectrometry (basics)</li><li>Quantitative analysis.</li></ul>Practical part:QTRAP:<ul><li>QTRAP scanning modes on the instrument - Q1, , MS2, MRM, demonstration and discussion on the example of a selected pesticide of the fragmentation spectrum and its fragmentation pathway.</li><li>MRM pair formation (manual, semi-automatic, automatic).</li><li>optimization of ion source parameters -T-conector</li><li>explanation of the main parameters related to LC (column selection, gradient vs isocrat, autosampler)</li><li>LC-MS/MS method setup and optimization</li></ul>QTOF:<ul><li>manual and automatic optimization of MRMHR</li><li>ion source and LC parameters (transfer from method developed above)</li></ul>Sequence of quantitative analysis on both instruments.Day IIQuantitative analysis:<ul><li>data processing and analysis,</li><li>reporting - Sciex OS (QTRAP, QTOF).</li><li>Discussion of types of integration algorithms (MQ4, autopeak) and integration parameters.</li><li>independent selection of integration parameters and plotting of master curves.</li><li>independent data analysis and generation of analysis report.</li></ul>Qualitative analysis:<ul><li>Theoretical introduction - introduction to qualitative analysis:</li><li>qwadrupole modes (Prec/NL),</li><li>trap modes (EMS, EPI, MS3),</li><li>QTOF modes (TOF MS, TOF MS/MS).</li><li>mixed methods,</li><li>targeted vs. untargeted analysis,</li><li>DDA (IDA) - discussion of method development,</li></ul>Practical part:<ul><li>creation of qualitative methods on QTRAP (Prec-IDA, MRM-IDA), other parameters (LC, ion source) as the previous day,</li><li>LightSight - discussion of operation, creation of MRM-IDA multimethod for selected pesticide and analysis of samples (night).</li><li>QTOF - creation of IDA method, other parameters (LC, ion source) as the previous day.</li></ul>Sequence of qualitative analysis overnight.Day IIIQualitative data analysis:QTRAP:<ul><li>Discussion of Prec-IDA, MRM-IDA results - what we see in different types of MS and MS/MS scans. Manual review of data and via IDA Explorer (SciexOS).</li><li>Analysis of results in LightSight, comparison with Prec-IDA, for example - advantages/disadvantages</li></ul>QTOF:<ul><li>search for potentially present compounds in the sample,</li><li>manual comparison of blank vs. test sample,</li><li>manual by XIC extraction (by sum formula),</li><li>Formula finder - derivation of sum formula on TOF MS data and verification of fragmentation on TOF MS/MS data</li><li>search in IDA Explorer mode - application of filters: similarity, isotopic distribution, mass defect,</li><li>database searching,</li><li>Molecule Profiler - demonstration of software capabilities on selected pesticide example,</li></ul>Instrument calibration:<ul><li>resolution, mass-to-charge ratio, intensity,</li><li>Q1 and Q3, LIT (MS and MS/MS), TOF MS and TOF-MS/MS</li><li>Automation vs. manual calibration,</li></ul>LC-MS/MS application examplesSummaryDiscussion

Bioanalytic Quantitative LC-MS/MS analysis training

The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis.

<h4>BASIC INFORMATION</h4><ul><li>TITLE: Quantitative LC-MS/MS analysis training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL</li><li>TRAINING TIME: 3 days</li><li>NUMBER OF PARTICIPANTS: up to 5 people</li><li>EQUIPMENT: SCIEX QTRAP LC-MS/MS</li></ul><figure class="image"><img style="aspect-ratio:1200/675;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg" alt="Bioanalytic: Bioanalytic Quantitative LC-MS-MS analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_Quantitative_LC_MS_MS_analysis_training_d13b5d8127.jpg 245w," sizes="100vw" width="1200" height="675"></figure><h4>TRAINING DESCRIPTION</h4>Quantitative LC-MS/MS analysis - the training is designed for laboratory personnel using LC-MS/MS systems for quantitative analysis. The following topics are covered during the training:<ul><li>Triple quadrupole spectrometer calibration</li><li>MRM pair formation and optimization</li><li>Optimization of ion source parameters</li><li>Optimization of chromatographic parameters</li><li>Sample preparation using the example of blood drawn on <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1283">Mitra®</a> samplers (VAMS)</li><li>Matrix effect, internal standards (ISTD), matrix curves</li><li>Validation of the quantitative method</li><li>Reporting of results</li></ul>The goal of the training is to introduce users to the procedures and capabilities of the LC-MS/MS system and software to achieve sensitivity, selectivity, precision and reproducibility in quantitative analysis while covering the widest possible linear range of concentrations.<h3>Training program</h3><h4>Day I</h4><h5>Theoretical part:</h5>Triple quadrupole structure, ionization, MRM and sMRM scanning, chromatography, multimethods<h5>Practical part:</h5><ul><li>Q1/Q3 calibration (10 Da/s), MS/MS - resolution, m/z, intensity.</li><li>MRM pair formation and optimization (manual, semi-automatic, automatic).</li><li>Ion source optimization - T-concector and FIA</li><li>Gradient vs isocrat, dosing, column selection, temperature selection, other important parameters</li><li>Establishment and optimization of LC-MS/MS method.</li><li>How to take care of MS cleanliness - ionization time programming, multi-way valve, cleaning of ion source and instrument front end</li></ul>Sample preparation in pure solvent (standard curve, QC, solvent, solvent+ISTD, test samples), overnight analysis sequence.<h4>Day II</h4><h5>Practical part - preparation of whole blood samples (Mitra®):</h5><ul><li>Preparation of matrix standard curves</li><li>Preparation of quality control (QC) samples in three concentrations</li><li>Preparation of blank samples (without ISTD and with ISTD).</li><li>Preparation of samples for recovery determination (“pre-spiked” and “post-spiked”)</li><li>Preparation of test samples</li></ul><h5>Theoretical part:</h5><ul><li>Samples in the matrix - discussion of the matrix effect and ways to level it.</li><li>Optimization of sample preparation method and LC-MS/MS method</li></ul><h5>Practical part:</h5><ul><li>Sample extraction from Mitra® samplers for LC-MS/MS analysis.</li><li>Sequence of analysis of prepared samples overnight</li></ul><h4>Day III</h4><h5>Theoretical part:</h5><ul><li>Validation of the quantitative LC-MS/MS method</li></ul><h5>Practical part:</h5><ul><li>Software - data integration algorithms, signal smoothing, weighing.</li><li>Matrix curves vs curves in solvent, influence of ISTD (Sciex OS).</li><li>Method validation - validation criteria, determination of LOD, LLOQ, ULOQ, linearity, working range of the method, repeatability, recovery.</li><li>Preparation of summary validation reports</li><li>Reporting of results from test samples - report vs export of data to Excel.</li><li>Summary and discussion.</li></ul>

Bioanalytic QTRAP vs QTOF Quality analysis training

The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis.

<h4>BASIC INFORMATION</h4><ul><li>TITLE: QTRAP vs QTOF Quality analysis training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL</li><li>TRAINING TIME: 3 days</li><li>NUMBER OF PARTICIPANTS: up to 5 people</li><li>EQUIPMENT: SCIEX QTRAP &amp; QTOF LC-MS/MS</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg" alt="Bioanalytic: Bioanalytic QTRAP vs QTOF Quality analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTRAP_vs_QTOF_Quality_analysis_training_18ca66163f.jpg 221w," sizes="100vw" width="1000" height="707"><h4>TRAINING COURSE DESCRIPTION</h4>QTRAP vs QTOF qualitative analysis - the training is aimed at people who perform analyses related to substance identification or metabolite search in their research work. The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis. In addition to the theoretical session, participants will undergo a course in the development and optimization of DDA (IDA) (QTRAP &amp; QTOF) and DIA (SWATH) (QTOF) methods. Analysis and comparison of results will be conducted using <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/sciex-os-software">SciexOS</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/markerview-software">MarkerView</a>, <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/lightsight-software">LightSight</a> and <a target="_blank" rel="noopener noreferrer" href="https://sciex.com/products/software/molecule-profiler-software">MoleculeProfiler</a> software.<h5>Training program</h5><h4>Day I</h4><h5>Theoretical part</h5><ul><li>Principle of operation of MS/MS systems</li><li>Differences and capabilities of QTRAP and QTOF</li><li>Comparison of typical analytical procedures and their application</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - LightSight, Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4>Day II</h4>Objective - to develop methods to search for metabolites of a known substance.<h5>QTRAP</h5><ul><li>Development of IDA screening methods (pMRM, Prec/NL, EMS)</li><li>Optimization of LC-MS/MS IDA method</li><li>Data acquisition</li></ul><h5>QTOF</h5><ul><li>Development and optimization of LC-MS/MS IDA and SWATH methods</li><li>Data acquisition</li></ul><h4>Day III</h4>Objective - identification and profiling of metabolites of a known substance<h5>QTRAP data analysis</h5><ul><li>Manual search and identification</li><li>LightSight, MarkerView</li></ul><h5>QTOF data analysis</h5><ul><li>Search and data analysis methods</li><li>Manual search and identification</li><li>IDA vs SWATH</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li></ul>Summary and discussion &nbsp;

Bioanalytic QTOF quantitative and qualitative analysis training

The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis.

<h4>BASIC INFORMATION</h4><ul><li>TITLE:&nbsp; QTOF quantitative and qualitative analysis training</li><li>CATEGORY: LC-MS/MS training</li><li>LOCATION: Lodz, PL</li><li>TRAINING TIME: 3 days</li><li>NUMBER OF PARTICIPANTS: up to 5 people</li><li>EQUIPMENT: SCIEX QTOF LC-MS/MS</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg" alt="Bioanalytic: Bioanalytic QTOF quantitative and qualitative analysis training." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Bioanalytic_Bioanalytic_QTOF_quantitative_and_qualitative_analysis_training_af1620ae8c.jpg 221w," sizes="100vw" width="1000" height="707"><h4>TRAINING COURSE DESCRIPTION</h4>Qualitative and quantitative analysis of QTOF - the training is designed for laboratory personnel using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=30&amp;mainauthorItems=55">high-resolution (HR) LC-MS/MS</a> systems for qualitative and quantitative analysis. The following topics are covered during the training:<ul><li>QTOF spectrometer calibration</li><li>Targeted and untargeted methods</li><li>Quantitative analysis (TOF MS, MRMhr)</li><li>Qualitative and quantitative analysis - DDA (IDA) vs DIA (SWATH) methodology</li><li>Software and its use in high-resolution data analysis</li><li>Reporting of results</li></ul>The purpose of the training is to introduce users to the capabilities of the LC-HR-MS/MS (QTOF) system in quantitative and qualitative analysis. To point out the advantages of high resolution in identifying or confirming the presence of substances in a sample and also the strengths of modern QTOF systems in parallel quantitative analysis.<h3>Training program</h3><h4>Day I</h4><h5>Theoretical part</h5><ul><li>The principle of operation and capabilities of the QTOF system</li><li>Comparison of typical analytical procedures and their application</li><li>Targeted and quantitative analysis (TOF MS vs MRMhr)</li><li>DDA (IDA) vs DIA (SWATH) methodology.</li><li>Supporting software - Molecule Profiler, ACD Labs, MarkerView</li><li>General principles of compound fragmentation</li></ul><h4>Day II</h4>Objective - to develop methods for quantitative analysis, confirmation or search for metabolites of a known substance, general characterization of the sample.<h5>Practical part:</h5><ul><li>Creation and optimization of the MRMhr targeted method</li><li>Creation and optimization of a non-targeted method IDA</li><li>Creation and optimization of the non-targeted method SWATH</li><li>Data acquisition (overnight)</li></ul><h4>Day III</h4>Objective - quantitative analysis, confirmation of the presence or identification of metabolites of a known substance, general characterization of the sample (identification of compounds present in the sample), profiling.<h5>Practical part:</h5><ul><li>Quantitative analysis - TOF MS vs MRMhr</li><li>Methods for searching and analyzing data</li><li>IDA vs SWATH data</li><li>Manual search and identification</li><li>Automated search and identification - SciexOS, databases</li><li>Molecule Profiler, MarkerView</li><li>Summary and discussion</li></ul>

Products from category Trainings - page 1

Related content

Phosphorylation, ADMA, and O-GlcNAc Analysis on the Orbitrap Excedion Pro Mass Spectrometer

ISC 2026 Starts in One Week: What You Need to Know

Webinars LabRulezLCMS Week 36/2026