BASIC INFORMATION
- TITLE: QTRAP vs QTOF Quality analysis training
- CATEGORY: LC-MS/MS training
- LOCATION: Lodz, PL
- TRAINING TIME: 3 days
- NUMBER OF PARTICIPANTS: up to 5 people
- EQUIPMENT: SCIEX QTRAP & QTOF LC-MS/MS
Bioanalytic: Bioanalytic QTRAP vs QTOF Quality analysis training.
TRAINING COURSE DESCRIPTION
QTRAP vs QTOF qualitative analysis - the training is aimed at people who perform analyses related to substance identification or metabolite search in their research work. The training includes a presentation of the basic differences in application and a comparison of the capabilities of QTRAP and QTOF instruments in qualitative analysis. In addition to the theoretical session, participants will undergo a course in the development and optimization of DDA (IDA) (QTRAP & QTOF) and DIA (SWATH) (QTOF) methods. Analysis and comparison of results will be conducted using SciexOS, MarkerView, LightSight and MoleculeProfiler software.
Training program
Day I
Theoretical part
- Principle of operation of MS/MS systems
- Differences and capabilities of QTRAP and QTOF
- Comparison of typical analytical procedures and their application
- DDA (IDA) vs DIA (SWATH) methodology.
- Supporting software - LightSight, Molecule Profiler, ACD Labs, MarkerView
- General principles of compound fragmentation
Day II
Objective - to develop methods to search for metabolites of a known substance.
QTRAP
- Development of IDA screening methods (pMRM, Prec/NL, EMS)
- Optimization of LC-MS/MS IDA method
- Data acquisition
QTOF
- Development and optimization of LC-MS/MS IDA and SWATH methods
- Data acquisition
Day III
Objective - identification and profiling of metabolites of a known substance
QTRAP data analysis
- Manual search and identification
- LightSight, MarkerView
QTOF data analysis
- Search and data analysis methods
- Manual search and identification
- IDA vs SWATH
- Automated search and identification - SciexOS, databases
- Molecule Profiler, MarkerView
Summary and discussion
