A bead-based calibration workflow enables quantitative multielement analysis of single cells by mass cytometry and supports a broad range of lanthanide tags.

Propionic acid increases peptide identifications by 39% versus formic acid in LC-MS proteomics while maintaining chromatographic performance and compatibility.

A GC-MS/MS method quantifies inorganic fluoride in EOF extracts and shows that fluoride contamination can substantially overestimate organic fluorine measured by CIC.

A study in Nucleic Acids Research shows that short start–stop elements act as highly effective translation gates, helping control protein synthesis even under cellular stress.

CEITEC-led researchers developed a computational method to design antimicrobial peptides that form pores in bacterial membranes and kill drug-resistant pathogens, with efficacy confirmed in mouse models.

SQUAD metabolomics combines targeted quantitation and untargeted discovery in a single Orbitrap MS experiment, delivering confident quantitative results alongside broad metabolite profiling.

This study introduces negative-ion single-event ICP-MS for direct particle-resolved detection and sizing of PTFE and PVC microplastics via F⁻ and Cl⁻ ions.

A two-stage liquid–liquid extraction method enables sensitive, reproducible analysis of ultrashort PFCAs such as TFA and longer-chain PFAS in water.

A cryogenic single-column GC-MS method with modified EI enables direct hydrogen quantification in complex gas mixtures using helium carrier gas.

This review explores lesser-known Czech contributions to chromatography and electrophoresis and highlights recent advances in separation science and instrumentation.