<p><strong>For the first time, thorium nuclei have now been successfully transferred from one state to another using a laser – but there has already been speculation for decades about the technical possibilities that would arise if this feat were one day possible. Expectations are high: probably the best-known idea is the nuclear clock, but precision measurements based on the thorium transition could open up new possibilities in completely different areas of research.</strong></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/TU_Wien_Nuclear_Clocks_and_the_Fundamental_Constants_of_Nature_The_nuclear_clock_a_spectacular_application_made_possible_by_the_discovery_of_the_thorium_transition_cbfb93c6fb.jpg" alt="TU Wien: The nuclear clock: a spectacular application, made possible by the discovery of the thorium transition."/></p>
<h4><strong>Time measurement</strong></h4>
<p>The most accurate time measuring devices today are atomic clocks – they have even changed the definition of our units of time. Today, the second is defined as the length of time corresponding to a very specific number of oscillations of the light that must be shone on a caesium atom in order to switch its electrons from one state to another.</p>
<p>Similar to the pendulum of a pendulum clock, the light oscillation of the caesium atoms plays the role of the timer that provides the regular ticking for the most accurate time measurement possible. Today, atomic clocks are used, for example, for the coordination of satellites, they enable the high accuracy of GPS signals and they also play a role in telecommunications.</p>
<p>Instead of cesium atoms, however, thorium atomic nuclei can now also be used for keeping time. Only when the thorium nuclei are irradiated with a laser beam of exactly the right frequency do they change their state. If the laser changes its frequency slightly (e.g. due to external interference), this immediately results in a change of the response of the thorium nuclei. The combination of laser and thorium nuclei therefore makes it possible to keep the laser frequency extremely stable and ensures that the laser frequency does not drift away.</p>
<p>Because atomic nuclei are much smaller than atoms and much less sensitive to electromagnetic perturbations, atomic nuclei can achieve much higher precision than atoms when it comes to measuring time.</p>
<h4><strong>Everything gets better with better clocks</strong></h4>
<p>With better time measurement, other physical quantities can also be measured more accurately. This is a well-known phenomenon: in nautical navigation, for example, people have struggled for a long time with the problem that although it is easy to determine the latitude based on the position of the sun, an accurate clock is required to calculate the longitude correctly. Better time measurement often makes other measurements possible.</p>
<p>For example, Einstein&#39;s theory of relativity states that time does not pass in the same way everywhere: the flow of time depends on the gravitational field. In this way, an extremely precise clock could therefore be used to accurately measure the Earth&#39;s gravitational field – with many possible applications ranging from the search for mineral resources to research into plate tectonics and earthquake prediction.</p>
<p>Great hopes are also pinned on the possibility that new, better clocks could clarify previously unsolved fundamental questions of physics: If it is possible to measure the constants of nature much more accurately than before, then it would also be possible to test the theory that these natural constants may not be perfectly constant at all. Perhaps they change over time? Research into dark matter is also hoping to gain new insights through even more precise measurements.</p>
<p>It is not always possible to get closer to the fundamental laws of nature just by looking at the smallest particles in a particle accelerator or by looking at the most distant regions of space. Sometimes you just need greater precision – and this is where the newly discovered thorium transition and all its potential applications should help in the coming years and decades.</p>
<h4><strong>Back to the main article:</strong></h4>
<ul>
<li><a href="https://gcms.labrulez.com/article/4273"><strong>Atomic Nucleus Excited with Laser: A Breakthrough after Decades</strong></a></li>
</ul>


Nuclear Clocks and the Fundamental Constants of Nature

<p><strong>Vaping aerosols contain thousands of unknown chemicals and substances not disclosed by manufacturers, including industrial chemicals and caffeine, Johns Hopkins University researchers found.</strong></p>
<p><strong>The study is the first to apply to vaping liquids and aerosols an advanced fingerprinting technique used to identify chemicals in food and wastewater. The results, <a href="https://pubs.acs.org/doi/10.1021/acs.chemrestox.1c00253">just published in the journal <em>Chemical Research in Toxicology</em></a>, suggest people who vape are using a product whose risks have yet to be fully determined and could be exposing themselves to chemicals with adverse health effects.</strong></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Johns_Hopkins_University_WILL_KIRK_JOHNS_HOPKINS_RESEARCHERS_FIND_THOUSANDS_OF_UNKNOWN_CHEMICALS_IN_ELECTRONIC_CIGARETTES_9b00c7b9b3.jpg" alt="Johns Hopkins University / Will Kirk: Johns Hopkins Researchers Find Thousands of Unknown Chemicals in Electronic Cigarettes."/></p>
<p><em>&quot;Existing research that compared e-cigarettes with normal cigarettes found that cigarette contaminants are much lower in e-cigarettes. The problem is that e-cigarette aerosols contain other completely uncharacterized chemicals that might have health risks that we don&#39;t yet know about,&quot;</em> said senior author <a href="https://engineering.jhu.edu/ehe/faculty/carsten-prasse/"><strong>Carsten Prasse</strong></a>, an assistant professor of environmental health and engineering at the <a href="https://engineering.jhu.edu/"><strong>Whiting School of Engineering</strong></a> and the <a href="https://publichealth.jhu.edu/"><strong>Bloomberg School of Public Health</strong></a>. <em>&quot;More and more young people are using these e-cigarettes and they need to know what they&#39;re being exposed to.&quot;</em></p>
<p>Previous studies of e-cigarettes have looked specifically for evidence of the hazardous chemicals found in traditional cigarettes. But here the researchers performed a nontargeted analysis to explore the full range of chemicals both in the vaping liquid and the aerosols.</p>
<p>Prasse and his team were the first researchers to test vape samples with chromatography/high-resolution mass spectrometry, a chemical fingerprinting technique more commonly used to identify organic compounds in wastewater, food, and blood. Although it&#39;s possible to buy vaping products in hundreds of flavors, for consistency they tested only tobacco-flavored liquids sold by four popular brands—Mi-Salt, Vuse, Juul, and Blu. They found thousands of unknown chemicals in the liquid, and the number of compounds increased significantly in the aerosol. Furthermore, they detected condensed hydrocarbon-like compounds, typically associated with combustion, which manufacturers say is not happening during vaping. In traditional cigarettes, the condensed hydrocarbons generated during combustion are toxic.</p>
<blockquote>
<h4>&quot;I HAVE A PROBLEM WITH HOW VAPING IS BEING MARKETED AS MORE HEALTHY THAN SMOKING CIGARETTES. IN MY OPINION WE ARE JUST NOT AT THE POINT WHEN WE CAN REALLY SAY THAT.&quot;</h4>
</blockquote>
<blockquote>
<p>Carsten Prasse</p>
<h6>Assistant professor, School of Engineering</h6>
</blockquote>
<p><em>&quot;One of the main ways electronic cigarettes have been marketed is that they operate at temperatures below combustion, which would make them safer than traditional smoking,&quot;</em> said lead author Mina Tehrani, a postdoctoral fellow in the School of Public Health. <em>&quot;Our study shows that this novel fingerprinting approach can be applied to assess whether combustion-like processes are going on.&quot;</em></p>
<p>The team found nearly 2,000 chemicals, the vast majority of which are unidentified. Of those the team could identify, six substances were potentially harmful, including three chemicals never previously found in e-cigarettes. Tehrani was particularly surprised to find the stimulant caffeine in two of the four products. Caffeine has previously been detected in e-cigarettes but only in the caffeine-oriented flavors like coffee and chocolate.</p>
<p><em>&quot;That might be giving smokers an extra kick that is not disclosed,&quot;</em> she said. <em>&quot;We wonder if they are adding it intentionally.&quot;</em></p>
<p>Besides caffeine, the team found three industrial chemicals, a pesticide, and two flavorings linked with possible toxic effects and respiratory irritation.</p>
<p>Prasse said he became interested in studying vaping products after his cousin, a former smoker, started vaping, insisting it was healthy. He plans to send that cousin this paper.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Johns_Hopkins_University_WILL_KIRK_JOHNS_HOPKINS_RESEARCHERS_FIND_THOUSANDS_OF_UNKNOWN_CHEMICALS_IN_ELECTRONIC_CIGARETTES_1_f38915afda.jpg" alt="Johns Hopkins University / Will Kirk: Johns Hopkins Researchers Find Thousands of Unknown Chemicals in Electronic Cigarettes."/></p>
<p><em>&quot;People just need to know that they&#39;re inhaling a very complex mixture of chemicals when they vape. And for a lot of these compounds we have no idea what they actually are,&quot;</em> Prasse said. <em>&quot;I have a problem with how vaping is being marketed as more healthy than smoking cigarettes. In my opinion we are just not at the point when we can really say that.&quot;</em></p>
<p>Co-author Ana M. Rule, an expert in metals exposures from vaping with the Johns Hopkins Bloomberg School of Public Health, says many young people who vape never smoked—so they aren&#39;t making a healthier choice, only starting out with a risky one.</p>
<p><em>&quot;There are millions of middle school and high school students vaping that would not otherwise think of smoking,&quot;</em> she says. <em>&quot;For them there is no risk reduction, only increased risk.&quot;</em></p>
<p>Authors also include Matthew N. Newmeyer, a postdoctoral fellow at the Johns Hopkins Bloomberg School of Public Health. The work was supported by grants from the National Institute of Environmental Health Sciences, the National Center for Advancing Translational Sciences, and NIH Roadmap for Medical Research.</p>


Johns Hopkins Researchers Find Thousands of Unknown Chemicals in Electronic Cigarettes

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezGCMS Library in the week of 22nd July 2024? Check out new documents from the field of the gas phase, especially GC and GC/MS techniques!</strong></p>
<blockquote>
<h4>👉 <a href="https://gcms.labrulez.com/library"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT GCMS AND RELATED TECHNIQUES</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p>
</blockquote>
<p>This week we bring to you poster presentations from <a href="https://gcms.labrulez.com/events/568"><strong>ASMS 2024</strong></a> by <strong>LECO</strong>, <strong>Agilent Technologies</strong>, and <strong>Thermo Fisher Scientific</strong>! And as a bonus two application notes from <strong>Shimadzu</strong> and <strong>Thermo Fisher Scientific</strong>.</p>
<blockquote>
<p>💡 <strong>+ 100 ASMS posters and presentations <a href="https://gcms.labrulez.com/library?mainauthorItems=284">available in the LabRulezGCMS library</a></strong> (unique search and filtering)</p>
</blockquote>
<h4><strong>1. LECO: <a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Long_Lifetime_Ion_Detector_GC_G_Cx_GC_TOFMS_MS_ASMS_24_LPSS_326_4c9e1fb7a1.pdf">A Novel Long-Lifetime Ion Detector for GC-TOFMS and GCxGC-TOFMS with Sub-Femtogram Detection Limits</a></strong></h4>
<ul>
<li>poster - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Ion detection efficiency and fast response times are critical aspects of the ion detectors used in time-of-flight mass spectrometry (TOFMS). With modern advancements in ionization, ion optics, and ion transfer efficiency, TOFMS systems are capable of generating high ion flux onto the ion detectors. Having an ion detector that is capable of maintaining optimal performance over a long period of time while accepting high amounts of ion flux is a significant challenge in development of TOFMS instrumentation. Improvements in detector life will minimize maintenance costs and improve TOFMS user experience. A novel long-lifetime ion detector was developed in partnership with El Mul Technologies for use in LECO’s Pegasus® BTX GC and GCxGCTOFMS. The system was thoroughly evaluated for sensitivity and robustness, including longevity of the detector.</p>
<p><strong>Conclusions:</strong> The Pegasus BTX GC(GCxGC)-TOFMS with a novel Long-Lifetime Ion Detector has demonstrated the following performance characteristics:</p>
<ul>
<li>Sub-2 fg IDL for GC-TOFMS and Sub-fg IDL for GCxGC-TOFMS </li>
<li>Linear dynamic range of 5 orders of magnitude in GC-TOFMS and 4 orders of magnitude in GCxGC-TOFMS</li>
<li>Robust operation: the detector bias remains consistent and BTX peak intensity remains consistent over long periods of time, even with large numbers of samples acquired and significant amounts of charge accumulation on the detector.</li>
</ul>
<h4><strong>2. Thermo Fisher Scientific: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/po_142_utilizing_hydrogen_pesticides_food_gc_ms_po142_asms2024_en_7ed0951b93.pdf">Utilizing hydrogen carrier gas for sensitive analysis of pesticides in food using gas chromatography mass spectrometry</a></strong></h4>
<ul>
<li>poster - ASMS 2024</li>
</ul>
<h5><strong>Abstract</strong></h5>
<p><strong>Purpose:</strong> To demonstrate the performance of the Thermo Scientifc HeSaverH2Safer kit for iConnect split-splitless (SSL) injection module for trace analysis of pesticides using H2 as a carrier gas as a safe and sustainable solution for laboratory operations.</p>
<p><strong>Methods:</strong> In this study, hydrogen was used as a carrier gas for GC-MS/MS analysis of trace levels of pesticides in food. The GC was fitted with a new hydrogen safer split/splitless injector, with chromatographic separation performed on a TG-5 SIL MS(30 m × 0.25 mm, 0.25 μm) capillary GC column. The chromatographic conditions applied ensured critical pair separation was achieved. The GC-MS/MS system was equipped.</p>
<h5><strong>Conclusions</strong></h5>
<ul>
<li>The use of the HeSaver-H2Safer technology allows for a safe and compliant use of hydrogen as an alternative carrier gas in GC/GC-MS applications without the need to install a hydrogen sensor and removes any risk of unwanted reactions with the sample in the hot SSL injector.</li>
<li>When used with hydrogen, the limited carrier gas consumption offered by the HeSaverH2Safer mode permits a very controlled system demand for hydrogen, making this solution ideal for laboratories working with hydrogen generators.</li>
<li>Migrating the GC-MS/MS method from helium to hydrogen requires an adaptation of method parameters to address (i.e., retention time shift and different fragmentation patterns).</li>
<li>97% of pesticides in baby food and 98% in honey showed a linear response in the concentration range 0.005–0.500 mg/kg and the relative standard deviation for N=5 repeats of each sample type at 0.01 mg/kg was equal or lower than 10% for 98% of the evaluated compounds with an advanced electron ionization (AEI) source to increase sensitivity for analyte detection. Quantification and assessment of critical regulatory requirements, such as linearity and sensitivity, were performed in a single software.</li>
</ul>
<h4><strong>3. Agilent Technologies: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/po_142_utilizing_hydrogen_pesticides_food_gc_ms_po142_asms2024_en_7ed0951b93.pdf">Analyzing Nitrosamines with Hydrogen Carrier Gas: GC/MS/MS Analysis of Nitrosamines in Sartan Drugs</a></strong></h4>
<ul>
<li>Poster Reprint - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> The limited availability of helium has been a concern, leading to a growing interest in switching to alternative carrier gases like hydrogen. Hydrogen offers several advantages over helium1 , including faster run times and improved chromatographic resolution. Helium is commonly used as a carrier gas for analyzing nitrosamine impurities in pharmaceuticals. However, there are concerns that nitrosamines may react with hydrogen, forming unwanted byproducts. To ensure that using hydrogen as a carrier gas does not impact spectral quality, we evaluated its suitability for analyzing eight impurities:</p>
<ul>
<li>N-nitrosodimethylamine (NDMA),</li>
<li>N-nitrosomethylethylamine (NMEA),</li>
<li>N-nitrosodiethylamine (NDEA),</li>
<li>N-nitroso-ethylisopropylamine (NEIPA),</li>
<li>N-nitrosodiisopropylamine (NDIPA),</li>
<li>N-Nitrosodipropylamine (NDPA),</li>
<li>N-nitrosodi-n-butylamine (NDBA),</li>
<li>N-Nitrosopiperidine (NPIP).</li>
</ul>
<p>Hydrogen carrier gas was used with the 7000E and 7010 Series GC/TQ systems equipped with the HydroInert and HES ion sources respectively, assessing spectral quality, linearity, repeatability, recovery, and compliance with nitrosamine analysis regulations.</p>
<p>MassHunter 13 software suite provided the tools for data acquisition and processing to be performed under a unified compliance environment. OpenLab Electronic Content Management (ECM) XT includes native tools to facilitate compliance, including the ability to create users with affiliated permissions, the generation of audit trails, and remote data storage.</p>
<p><strong>Conclusions:</strong></p>
<ul>
<li>The 7000E and 7010 Series GC/TQ systems equipped with the HydroInert and HES, respectively, exhibited exceptional performance in determining eight nitrosamine drug impurities in sartan drug products and substances using hydrogen carrier gas.</li>
<li>The method was validated at 30 ppb with acceptable recovery and long-term repeatability. </li>
<li>The 7010 Series GC/TQ system enabled a smooth switch between helium and hydrogen carrier gas without compromising the performance of the methods. </li>
<li>Both the HydroInert and the HES sources facilitated achieving the necessary detection limits for the tested impurities.</li>
</ul>
<h4><strong>4. Agilent Technologies: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/po_142_utilizing_hydrogen_pesticides_food_gc_ms_po142_asms2024_en_7ed0951b93.pdf">Analysis of Essential Oils Using a Comprehensive GCxGC with a Reverse Flow Modulator Combined with High Resolution GC/MS</a></strong></h4>
<ul>
<li>Poster Reprint - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Essential oils are typically analyzed to determine their chemical composition contributing to flavor and quality as well as to evaluate their authenticity and identify adulterants. Essential oils are often complex samples with high number of volatile and semi-volatile components that include many isomers with highly similar spectra and close Retention Indices (RIs) when separated on a single column. Hence, a comprehensive GCxGC approach is preferable to achieve an appropriate chromatographic separation for this sample matrix. In this study we used comprehensive GCxGC coupled to a high-resolution accurate mass GC/MS to identify the essential oil  components. Here we also investigate approaches for comparison of the complex GCxGC essential oil data.</p>
<p><strong>Conclusions:</strong></p>
<ul>
<li>Several comprehensive GCxGC configurations and method using the high-resolution GC/Q-TOF and RFM were developed and optimized using a diesel sample. </li>
<li>Essential oils were analyzed using the optimized GCxGC approach, and the workflows for compound identification and statistical analysis were evaluated.</li>
</ul>
<h4><strong>5. Shimadzu: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/an_01_00746_en_e14cf49b7b.pdf">Greenhouse Gas Analyzer</a></strong></h4>
<ul>
<li>Application</li>
</ul>
<p><strong>User Benefits:</strong></p>
<ul>
<li>Simultaneously measures the 3 main greenhouse gases: CH₄, CO₂, and N₂O.</li>
<li>Offers short analysis cycle times and is equipped with an AOC-6000 Plus syringe injection system to analyze large numbers of samples.</li>
<li>Uses nitrogen as a carrier gas for lower running costs.</li>
</ul>
<p><strong>Introduction:</strong> Greenhouse gases are a major cause of climate change, and carbon dioxide, (CO₂), methane (CH₄), and nitrous oxide (N₂O) are considered the 3 primary greenhouse gases. Although CO₂ accounts for the majority of greenhouse gas emissions followed by CH₄, reducing CH₄ emissions is considered important because CH₄ has a warming effect that is more than twenty times greater than CO₂. At the United Nations Climate Change Conference (COP26) in November 2021, over 100 countries joined the Global Methane Pledge, which pledges by 2030 to reduce global CH₄ emissions by 30 % compared to 2020 levels. The Greenhouse Gas Analyzer is a gas chromatography system that has been developed by Shimadzu in collaboration with the National Agriculture and Food Research Organization (NARO) (Fig. 1). Measuring levels of CH₄, CO₂, and N₂O simultaneously in a single sample is difficult, but the Greenhouse Gas Analyzer has been designed to do this simultaneously, which reduces analytical errors and improves the sampling speed, giving researchers a valuable tool in reducing greenhouse gas emissions.</p>
<p>This Application News describes using the Greenhouse Gas Analyzer to simultaneously measure CO₂, CH₄, and N₂O levels.</p>
<p><strong>Conclusion:</strong> The Greenhouse Gas Analyzer can simultaneously measure 3 greenhouse gases in atmospheric air in a single analysis. Each analysis by the Greenhouse Gas Analyzer is performed in 8 minutes with an analysis cycle time of 10 minutes.</p>
<h4><strong>6. Thermo Fisher Scientific: <a href="https://gcms.cz/labrulez-bucket-strapi-h3hsga3/an_002695_gc_triplus_rsh_smart_sample_prep_environ_an002695_na_en_77a5aa2300.pdf">An automated approach for the analysis of VOCs in drinking and surface water by using the TriPlus RSH SMART VOC Sample Prep Station</a></strong></h4>
<ul>
<li>Application - Environmental</li>
</ul>
<p><strong>Goal:</strong> The aim of this study is to demonstrate the suitability of the Thermo Scientific™ TriPlus™ RSH SMART VOC Sample Prep Station for the analysis of volatile organic compounds (VOCs) in drinking and surface water by using a fully automated sample preparation workflow.</p>
<p><strong>Keywords:</strong> Automated sample preparation, TriPlus RSH SMART Sample Prep Station, volatile organic compounds, VOCs, drinking water, surface water, gas chromatography, TRACE 1610 GC, single quadrupole mass spectrometry, ISQ 7610 GC-MS.</p>
<p><strong>Conclusions:</strong> The results of these experiments demonstrate that the automated sample preparation capability of the TriPlus RSH SMART VOC Sample Prep Station coupled to the ISQ 7610 GC-MS system provides an ideal solution for water testing laboratories looking to improve productivity and deliver confident results.</p>
<ul>
<li>Static headspace is a convenient solventless extraction technique for volatiles in water with almost no sample preparation required. </li>
<li>Unattended operations of up to 210 samples can be achieved with the automated calibration dilution and ISTD addition workflows.</li>
<li>The automated addition of fresh reagent just before the incubation increases the stability of ISTD/surrogates mix, therefore improving the accuracy of the quantitation of target analytes during data reprocessing.</li>
<li>The TriPlus RSH SMART VOC Sample Prep Station ensures increased sample integrity for highly reliable quantitative analysis, and reduced errors or possible cross-contaminations, maximizing the productivity of the laboratory. Additionally, it allows saving valuable analyst time and improving safety by limiting the user’s exposure to toxic chemicals. </li>
<li>The integrated control for both autosampler and GC-MS in a single CDS ensures a streamlined automated workflow from on-line sample preparation to sequence setup, data acquisition, and reporting. </li>
<li>Suitability of headspace sampling for analysis of VOCs was demonstrated with R2  &gt; 0.990, RRF %RSD &lt; 20%, and calculated amount within 20% of the spiked concentration. </li>
<li>Inter-day reproducibility was demonstrated by running three batches of samples bracketed with QCs. Average absolute peak area %RSD across the entire evaluation period was &lt;20%, the QC calculated amount was within 20% of the expected value, and the calculated recovery was within 70–130%, with the only exception of 1,2-dibromo-3-chloropropane for which the % recovery was 132. </li>
<li>Reliable quantitative analysis was achieved for drinking water samples and surface water samples analyzed in three different batches across six working days. All the sample results were compliant with the thresholds set by the current EU regulation.</li>
</ul>


News from LabRulezGCMS Library - Week 30, 2024

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 22th July 2024? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p>
<blockquote>
<h4>👉 <a href="https://lcms.labrulez.com/library"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p>
</blockquote>
<p>This week we bring to you poster presentations from <a href="https://lcms.labrulez.com/events/568"><strong>ASMS 2024</strong></a> by <strong>Agilent Technologies</strong>, <strong>Shimadzu</strong>, <strong>Thermo Fisher Scientific</strong>, and <strong>Waters Corporation</strong>!</p>
<blockquote>
<p>💡 <strong>Almost 1000 ASMS posters and presentations <a href="https://lcms.labrulez.com/library?mainauthorItems=284">available in the LabRulezLCMS library</a></strong> (unique search and filtering)</p>
</blockquote>
<h4><strong>1. Waters Corporation: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/2023_asms_king_liver_178c54e990.pdf">HIGH THROUGHPUT PLASMA PROFILING OF HUMAN LIVER DISEASE SAMPLES USING RAPID CHROMATOGRAPHY AND A MULTI- REFLECTING TIME-OF-FLIGHT MASS SPECTROMETER</a></strong></h4>
<ul>
<li>poster - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Metabolomics is a valuable research strategy in the determination  FIG. 5  FIG. 1  FIG. 7  FIG. 3  of disease progression, disease stratification, the identification of  biological markers for early diagnosis and for the development of  new therapeutics. Acute-on-chronic liver failure (ACLF) is a  condition characterized by systemic inflammation, multiple organ  failure, and high short-term mortality1. ACLF develops from the  acute decompensation of cirrhosis and currently there is no  biomarker identified for the early diagnosis of this disease.  Therefore, fully understanding the role metabolites and lipids play  in the progression of ACLF and its underlining mechanisms could  greatly help determine new diagnostic markers driving  development of new therapeutics. Investigating biomarkers for  large scale studies requires robust, high-throughput analytical  methods.</p>
<p>Rapid microbore metabolic profiling (RAMMP) methods and  conventional high-resolution mass spectrometry have previously  been shown to considerably reduce analysis time2-3. Here we  compare these methods with a vacuum jacketed column  technology (VJC)4-5 and ultra high-resolution mass spectrometry6  for the analysis of plasma from liver disease patients by reversed-  phase (RP) for lipid and hydrophilic interaction chromatography  (HILIC) for small molecules.</p>
<p><strong>Conclusion:</strong></p>
<ul>
<li>Conventional LC-MS methods used for large cohort studies suffer from batch response variation.  The rapid chromatographic methods reduce this impact and demonstrated reproducible response  across the entire batch of 168 injections per polarity (Fig. 2).</li>
<li>The SELECT SERIES MRT mass spectrometer generated highly accurate mass measurements  (&lt;1 ppm), improving annotation confidence for both lipids and small molecules, including essential  amino acids and carnitines from the HILIC analysis (Fig. 3).</li>
<li>PCA of the RAMMP data showed good analytical reproducibility from the clustered QC samples and  group separation with some overlap between the different disease groups (Fig. 4).</li>
<li>Group comparisons were performed by OPLS-DA where statistically significant features were  highlighted for database annotation (Fig. 5).</li>
<li>A number of lipids classes, particularly lysophosphatidylcholines (LPC), phosphatidylcholines (PC)  and sphingomyelins (SM), showed dysregulation in the diseased samples when compared to healthy  controls and were annotated through Lipostar2 with mass accuracies between 0.1-0.3 ppm (Fig. 6).</li>
<li>For the HILIC small molecule analysis, differences in amino acid response were detected in  comparisons between diseased and healthy controls.</li>
<li>The SELECT SERIES MRT MS achieved mass resolution of 170-200K FWHM at 30 Hz compatible with  RAMMP (a) and VJC (b) methodologies for example lipids, PC36:1 (0.18 ppm) and SM42:3, O2 (0.16  ppm), with the VJC providing better peak shape and resolution of adjacent peaks (Fig. 7)</li>
<li>Comparison of the RAMMP and VJC negative lipid analysis showed very similar distribution of  disease and healthy control groups demonstrating minimal impact on the overall statistics (Fig. 8).</li>
<li>Both RAMMP and VJC methodologies combined with the SELECT SERIES MRT provided highly  accurate reproducible metabolic profiling for a biomarker study, with the VJC providing improved  peak fidelity complemented by the fast scanning high resolution capabilities of the SELECT SERIES  MRT.</li>
<li>This work demonstrated an accurate, reproducible and high throughput metabolomic method for the  rapid screening of a large number of biological samples for biomarker discovery.</li>
</ul>
<h4><strong>2. Thermo Fisher Scientific: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/po_143_characterization_structure_adenopeptin_lc_hram_ms_po143_asms2024_en_7ae893f36c.pdf">In-depth characterization and structure elucidation of non-ribosomal peptides related to adenopeptin using LC-HRAM-MSⁿ</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<h5><strong>Abstract</strong></h5>
<p><strong>Purpose:</strong> Characterization of adenopeptin and related peptaibol analogs in a crude fermentation broth extract using LC-HRAM-MSn to identify adenopeptin congeners and elucidate their structures. Methods: Adenopeptin and its analogs were separated using reversed phase chromatography and analyzed using multi-stage fragmentation on a Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer. </p>
<p><strong>Results:</strong> Several analogs of adenopeptin differing by one or more methyl groups could be detected and the site of their modification could be narrowed to specific amino acid residues based on their fragmentation patterns.</p>
<h5><strong>Conclusions</strong></h5>
<p>The LC/MS analysis of the crude adenopeptin extract revealed the presence of eight analogs above 0.5% relative MS-abundance, mainly resulting from ”demethylations”, i.e., substitution of Aib for Iva residues. Their modification sites could be determined based on shifts in the fragmentation pattern relative to adenopeptin. Additionally, a potentially crucial substitution of pipecolic acid with proline could be detected and verified based on MS3 spectral evidence for compound 4.</p>
<h4><strong>3. Thermo Fisher Scientific: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/po_146_evaluation_automated_low_flow_lc_ms_setup_po146_asms2024_en_1d935eb142.pdf">Evaluation of an Automated “Quick” Optimization Routine to Streamline Low-Flow LC-MS Setup</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<h5><strong>Abstract</strong></h5>
<p><strong>Purpose:</strong> When setting up low flow LC-MS experiments, a user regularly positions an  emitter at an arbitrary fixed distance from the inlet based on visual alignment. A  prototype ion source featuring an emitter mounted on a 3D motorized stage was used  to evaluate a “quick” optimization strategy used to define the active position for  subsequent LC-MS measurements.</p>
<p><strong>Methods:</strong> The basis of the quick optimization routine was established using m/z- and  position-dependent intensity distributions. The routine was evaluated against two  methods to manually set the emitter position.</p>
<p><strong>Results:</strong> All optimization strategies yielded a statistically equivalent number of protein  and peptide identifications. However, the quick optimization routine yielded superior  peak area reproducibility that translated into significantly better emitter-to-emitter  consistency.</p>
<h4><strong>4. Shimadzu: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/ASMS_poster_PESI_v_DMS_170524_fed75d0c1e.pdf">DPiMS-MS (PESI) combined with vacuum differential mobility spectrometry for rapid forensic analysis.</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Introduction</strong></p>
<ul>
<li>Ambient ionisation (AI) mass spectrometry techniques facilitate rapid turn-around of samples, often in a walk-up format with limited sample preparation, with obvious benefits for forensic applications.</li>
<li>The absence of a chromatographic separation enables sub-minute data acquisition. However, this comes with the cost of greater chance of problems with chemical noise and/or interference between isomeric/isobaric compounds.</li>
<li>One way to remove chemical noise and to separate interfering compounds is to add an ion mobility separation to the method.</li>
<li>Here we implement a novel low pressure differential mobility on a DPiMS-8060 (PESI) system and demonstrate performance for steroids and small molecule drugs in saliva.</li>
</ul>
<p><strong>Conclusion</strong></p>
<ul>
<li>These results demonstrate the potential of vDMS to enhance AIMS by removing chemical noise and separating isobaric species.</li>
<li>DPiMS-vDMS-8060 (PESI) retains the high acquisition speed of PESI while adding an additional dimension of separation.</li>
<li>The additional selectivity may lower the LOQ and reduce false positives.</li>
<li>vDMS-MS methods can be applied in a wide range of complex matrices (e.g. urine, plasma).</li>
</ul>
<h4><strong>5. Shimadzu: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/MP_045_Targeted_quantitative_screening_pesticides_using_high_resolution_DIA_spectral_library_matching_238a221a67.pdf">Targeted quantitative screening pesticides in food matrices using high resolution DIA spectral library matching.</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Overview</strong></p>
<ul>
<li>A single, generic untargeted data independent acquisition (DIA) method was developed for the analysis of extended panels of pesticides in food matrices.</li>
<li>The high-resolution LC-MS/MS method was designed for multi-residue screening and quantitation of pesticides to meet the European Union SANTE 11312/2021 v2 guidelines capable of working with extended target pesticide panels.</li>
<li>The MS and DIA-MS/MS method was applied to quantify a routine panel of pesticides but also included an additional target panel of fungicides for qualitative screening.</li>
</ul>
<p><strong>Conclusions</strong></p>
<ul>
<li>Using a single generic LC-MS and DIA-MS/MS method targeted quantitation using ion ratio confirmation (to meet the EU SANTE guidelines) and extended search capabilities with library identification were supported.</li>
<li>The method results in the quantitation of &gt;250 pesticides at or below the default MRL concentration (10 µg/kg).</li>
</ul>
<h4><strong>6. Shimadzu: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/MP_045_Targeted_quantitative_screening_pesticides_using_high_resolution_DIA_spectral_library_matching_238a221a67.pdf">Identification of positional isomers of linoleic acid containing phospholipids involved in pancreatic ductal adenocarcinoma.</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Overview</strong></p>
<ul>
<li>To enhance lipid identification, Oxygen Attachment Dissociation (OAD) MS/MS has been applied to provide double bond specific dissociation, enabling C=C position assignments.</li>
<li>High resolution OAD MS/MS was applied in positive and negative ion mode to provide further confidence in lipid identification in a pancreatic adenocarcinoma biomarker study</li>
</ul>
<p><strong>Conclusions</strong></p>
<ul>
<li>OAD-MS/MS is a high specificity fragmentation technique to enable C=C positional assignments in lipids. Analysis is easy to perform alongside CID-MS/MS and spectra are easy to interpret.</li>
<li>OAD-MS/MS has been applied to structurally characterize lipids found to be potential markers of PDAC in a previous study using high resolution metabolomics analysis by LC-CID-MS/MS and DPiMSᵗᵐ.</li>
<li>The use of this new technology aims to enhance pancreatic cancer research by providing more comprehensive identification needed to understand the biological roles of the lipids identified as potential markers of PDAC.</li>
</ul>
<h4><strong>7. Agilent Technologies: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/po_2dlc_6545xt_monitoring_oxidation_mabs_asms_2024_wp030_en_agilent_77f2bb18d5.pdf">Monitoring oxidation in recombinant monoclonal antibodies at subunit level through two-dimensional liquid chromatography coupled with mass spectrometry</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Oxidation, induced by reactive oxygen species, is a prevalent chemical modification in monoclonal antibodies (mAbs), affecting amino acid residues such as cysteine (C), methionine (M), tryptophan (W), tyrosine (Y), lysine (K), and others (Gupta et al., 2022). Each residue&#39;s oxidation uniquely influences the protein&#39;s structure and function. Methionine is highly prone to oxidation in mAbs, typically resulting in the formation of a methionine sulfoxide product with increased polarity. These methionine residues are found in both the antigen-binding (Fab) and effector activity (Fc) domains of a mAb, with those situated on the surface being particularly susceptible to oxidation (Hageman et al., 2019). The increasing popularity of two-dimensional liquid chromatography (2D-LC) has led to a significant rise in the integration of two complementary chromatographic techniques, coupled with mass spectrometry (MS), aiming to provide comprehensive insights into mAb post-translational modifications (PTMs) (Sarin et al., 2022). The proposed 2D WCX-RP-MS workflow enables the analysis of mAbs at the subunit level, providing an automated analytical platform for characterizing and identifying oxidized regions within both the Fc and Fab domains.</p>
<p><strong>Conclusions:</strong> The oxidized peaks not resolved by 1D methods are successfully resolved by the D2 RPC column with additional HC-LC information. It should be highlighted that the proposed method completely uses MS compatible buffers and is a faster method to identify mAb modification and their correlation in different mAb fragments compared to the tedious bottomup approach. A general mAb bottom-up analysis takes up to 24-48 hours with the risk of sample loss, degradation, and induced modifications. In the present study, sample preparation took only 4-10 hours (depending on oxidation incubation), and sample analysis took only 3 hours. Hence, 7-13 hours is enough for the subunit analysis with a low risk of sample loss and degradation. The present study focuses on oxidation as the chemical modification of interest, but the workflow can be extended to other critical charge modifications easily resolved by the WCX column.</p>
<h4><strong>8. Agilent Technologies: <a href="https://lcms.cz/labrulez-bucket-strapi-h3hsga3/po_2dlc_6545xt_monitoring_oxidation_mabs_asms_2024_wp030_en_agilent_77f2bb18d5.pdf">Flash Characterization of mAbs Using a Combination of Reagents via Automated On-Line Microdroplet Reaction</a></strong></h4>
<ul>
<li>Poster - ASMS 2024</li>
</ul>
<p><strong>Introduction:</strong> Recently, an unmodified Agilent Jet Stream ESI source (AJS) was used to demonstrate “Flash Characterization” of mAbs by microdroplet facilitated reductions or enzyme digestions with good reproducibility and high yield 1. These results motivated us to evaluate treatments that combined enzymatic digestion, reduction, and/or deglycosylation of mAb in a single microdroplet experiment. New optimization parameters for the Agilent Injection Program on Agilent 1290 Infinity II LC were presented. Microdroplet experiments, including GlycINATOR ® deglycosylation, the combination of IdeS cleavage and DTT reduction and IdeS cleavage and GlycINATOR ® deglycosylation were examined for reproducibility, intact mAb remaining after treatments and simplified subunit characterization. GlycINATOR is an endoglycosidase that cleaves N-linked glycans and leaves the core GlcNAc residue with or without a fucose on the asparagine. Additional antibodies of Herceptin and sigmamab are also studied with the combination treatment in the microdroplet reaction.</p>
<p><strong>Conclusions:</strong> </p>
<ul>
<li>Flash Characterization of mAbs with an automatic injector, combination treatments, and microdroplet reaction in the AJS source demonstrates simplification, reproducibility, and cost reduction of protein characterization. </li>
<li>Combination treatments are viable with microdroplet reactions to accelerate the reduction, cleavage, and deglycosylation of native IgG1 significantly.</li>
</ul>


News from LabRulezLCMS Library - Week 30, 2024

<p><strong>From individual cells and small tissue samples to fruit flies: if you want to research biological material, you often need very special equipment. The <a href="https://www.tuwien.at/en/koop/amt/projects/lifescope-3d">&quot;LifeScope3D&quot; platform</a> has now been set up at TU Wien to provide optimal instrumentation for studying 3D multicellular structures, such as organoids and spheroids. A whole range of specialized equipment is now available at the Getreidemarkt campus, which enables characterization on various scales- starting from entire objects down to single cell or molecule analysis. However, the platform is not only interesting for biological samples, but also for other similar-sized structures, such as those produced by a high-resolution 3D printer.</strong></p>
<p>Molecular biologist, Mina Petrovic, PhD, is the project manager of the new platform. LifeScope3D is the result of a collaboration between Prof. Aleksandr Ovsianikov (Institute of Materials Science and Technology), Prof. Philipp Thurner (Institute of Lightweight Structures and Structural Biomechanics) and Prof. Gerhard Schütz (Institute of Applied Physics). However, the platform will also be accessible to all other research groups - both within and outside TU Wien. LifeScope3D is intended to create a hub that promotes multidisciplinary collaborations in the field of cellular assemblies and tissue engineering. This was made possible by funding from the Austrian Research Promotion Agency FFG.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/TU_Wien_TU_Wien_launches_a_research_platform_for_3_D_testing_of_bio_samples_The_biosorter_in_the_new_lab_at_TU_W_Ien_d7b4ebaa9e.jpg" alt="TU Wien: The biosorter in the new lab at TU Wien."/></p>
<h4><strong>Different measuring devices</strong></h4>
<p><em>&quot;We can not only image biological structures, but also fully analyze them in three dimensions,&quot;</em> explains Mina Petrovic. <em>&quot;This is of great importance for many applications in biology.&quot;</em> For example, a special microscope on the new research platform can be used to illuminate the samples with so-called &quot;lattice light sheets&quot;. Instead of a beam of light that scans the sample point by point, an extremely thin, two-dimensional light sheet is generated that penetrates the sample layer by layer. Each time, only dyes located within this thin light sheet are detected and a three-dimensional image with high contrast can then be put together on the computer.</p>
<p>However, optical methods are not the only way to examine samples - mechanical experiments also play an important role. The sample can be subjected to a precisely defined mechanical load. While the sample is compressed, the sample deformation is measured. Valuable findings can be derived from this, as such experiments mimic the pressures that cells have to endure in different parts of the body.</p>
<p>A bio-sorter is also used in the new research platform: <em>&quot;Biological samples are rarely uniform, but often come in a mixture of different sizes, showing different features”</em>, explains Mina Petrovic. <em>&quot;In the bio-sorter, a mixture of, for example, multicellular spheroid structures is guided past a laser beam where each individual structure can be precisely analyzed and categorized at a very high speed.&quot;</em></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/TU_Wien_TU_Wien_launches_a_research_platform_for_3_D_testing_of_bio_samples_Computer_controlled_experiments_with_the_lattice_light_sheet_e11c405df2.jpg" alt="TU Wien: Computer controlled experiments with the lattice light sheet."/></p>
<h4><strong>Accessible for everybody</strong></h4>
<p>Numerous research groups from different faculties at TU Wien are conducting research on biological samples - LifeScope3D will open up new analysis methods for all of them. However, the aim is also to collaborate with research teams outside of TU Wien: &quot;In principle, LifeScope3D is open to all groups with suitable research projects,&quot; says Mina Petrovic. &quot;Anyone who wants to use our equipment can get in touch with us and arrange suitable timeslots; we will then also provide advice and help with the correct execution of the experiments.&quot;</p>
<p>The research is not necessarily limited to biological materials. &quot;The devices are ideally suited for biological analyses, but who knows - we may also receive exciting research requests from other disciplines that we might not even be thinking about today,&quot; says Mina Petrovic.</p>
<blockquote>
<h4><strong>Contact</strong></h4>
<p>Mina Petrovic, PhD</p>
<p>LifeScope3D</p>
<p>TU Wien</p>
<p>+43 1 58801 30892</p>
<p>mina.petrovic@tuwien.ac.at</p>
</blockquote>


TU Wien launches a research platform for 3D testing of bio-samples

<p><strong>In the 2 weeks from July 29, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p>
<blockquote>
<h4><a href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Markes International: Simplifying sample preparation for GC–MS</strong></h5>
<ul>
<li>Tu, 30.7.2024 12:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4131">Registration</a></li>
</ul>
<p>This webinar addresses the use of an innovative, robust and cost-effective sampling device, the Micro-Chamber/Thermal Extractor, combined with the thermal desorption GCMS.</p>
<h5><strong>2. Agilent Technologies: GC and GC/MS Frequently Asked Questions</strong></h5>
<ul>
<li>Tu, 30.7.2024 17:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/3896">Registration</a></li>
</ul>
<p>To help you improve your chromatographic know-how, we’ve compiled a list of some of our most frequently asked questions.</p>
<h5><strong>3. Agilent Technologies: Simplified Measurement of Content Distribution of Pharma Tablets</strong></h5>
<ul>
<li>Tu, 30.7.2024 21:30 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/3905">Registration</a></li>
</ul>
<p>Content distribution of entire pharma tablets can be quickly measured using the component-specific workflow of the LDIR.</p>
<h5><strong>4. Agilent Technologies: Analysis of Per- and Polyfluoroalkyl Substances in Different Media: Produce, Wastewater, and Air</strong></h5>
<ul>
<li>We, 31.7.2024 17:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4134">Registration</a></li>
</ul>
<p>This presentation will summarize analytical methods to determine PFAS concentrations in produce from the vicinity of a fluorochemical plant, wastewater, and air near wastewater treatment plants.</p>
<h5><strong>5. Phenomenex: Solid Phase Extraction Introduction and Theory</strong></h5>
<ul>
<li>Tu, 6.8.2024 19:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4159">Registration</a></li>
</ul>
<p>Understand the benefit of using SPE than other sample preparation techniques.</p>
<h5><strong>6. C&amp;EN/Metrohm: Smart and Actionable: Spectroscopy PAT-Supported Process Operations</strong></h5>
<ul>
<li>We, 7.8.2024 19:00 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4175">Registration</a></li>
</ul>
<p>How Albemarle Corporation approached the implementation of Spectroscopy PAT to obtain smarter process operations and what you can do to help drive a mindset change in your business.</p>
<h5><strong>7. Agilent Technologies: Advanced GC/MS | GC/HR-MS Based Workflow for Extractable and Leachable Analysis</strong></h5>
<ul>
<li>Th, 8.8.2024 11:30 CEST</li>
<li><a href="https://gcms.labrulez.com/webinar/4136">Registration</a></li>
</ul>
<p>Whether you are scouting or conducting a targeted screening, suspect screening, or nontargeted screening, Agilent can help with a wide range of analytical technologies.</p>


Webinars LabRulezGCMS Week 31-32/2024

<p><strong>In the week of July 29, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p>
<blockquote>
<h4><a href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. SCIEX: Excel strategies for mass spectrometry data analysis</strong></h5>
<ul>
<li>Tu, 30.7.2024 11:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/3857">Registration</a></li>
</ul>
<p>Elevate your analytical expertise with advanced techniques and workflows.</p>
<h5><strong>2. CTC Analytics: Advancing PFAS Analysis: Robotic Automation and Streamlined LC-MS Workflows</strong></h5>
<ul>
<li>We, 31.7.2024 09:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4137">Registration</a></li>
</ul>
<p>This webinar showcases the latest advancements in LC-MS workflows and robotic automation.</p>
<h5><strong>3. Waters Corporation: Exploring Enhanced Macromolecular and Nanoparticle Analysis through the Integration of DLS/ELS/SLS with the ZetaStar Instrument</strong></h5>
<ul>
<li>We, 31.7.2024 11:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4132">Registration</a></li>
</ul>
<p>The DynaPro ZetaStar instrument provides the capability for automated, unattended screening of a large range of sample and buffer conditions, which increases efficiency and reduces time to market.</p>
<h5><strong>4. Separation Science/SCIEX: PFAS Analysis in Food: A Robustness Study in Sensitivity and Stability</strong></h5>
<ul>
<li>We, 31.7.2024 11:30 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4112">Registration</a></li>
</ul>
<p>Learn how cutting-edge mass spec technology can co-exist with stability and robustness. It doesn’t have to be a compromise.</p>
<h5><strong>5. The Analytical Scientist/SCIEX: An approach to streamline and simplify the identification of crucial metabolites from targeted protein degraders</strong></h5>
<ul>
<li>We, 31.7.2024 15:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4110">Registration</a></li>
</ul>
<p>Fragment information from EAD and CID spectra were processed in a single result file to achieve confident structure assignment of and localization of metabolites.</p>
<h5><strong>6. Agilent Technologies: Analysis of Per- and Polyfluoroalkyl Substances in Different Media: Produce, Wastewater, and Air</strong></h5>
<ul>
<li>We, 31.7.2024 17:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4134">Registration</a></li>
</ul>
<p>This presentation will summarize analytical methods to determine PFAS concentrations in produce from the vicinity of a fluorochemical plant, wastewater, and air near wastewater treatment plants.</p>
<h5><strong>7. Waters Corporation: Structural characterization of functional polymers via light scattering and differential viscometry</strong></h5>
<ul>
<li>We, 31.7.2024 17:00 CEST</li>
<li><a href="https://lcms.labrulez.com/webinar/4111">Registration</a></li>
</ul>
<p>This webinar will explore polymer characterization by multi-angle light scattering (MALS) and differential viscometry, coupled with size-exclusion chromatography or field-flow fractionation.</p>


 Webinars LabRulezLCMS Week 31/2024

<p><strong>In the week from July 29, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p>
<blockquote>
<h4><a href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Bruker: Advances in Microscopy: A Gateway to Enhanced Research, Drug Development, and Diagnostics</strong></h5>
<ul>
<li>Mo, 29.7.2024 17:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4172">Registration</a></li>
</ul>
<p>Latest advancements in microscopy and applications in kidney disease research and diagnostics.</p>
<h5><strong>2. Bruker: Notch-Mutated Cancer Genomes in 3D</strong></h5>
<ul>
<li>Tu, 30.7.2024 17:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4173">Registration</a></li>
</ul>
<p>Find out what super-resolution microscopy can reveal about epigenetic changes in cancer pathogenesis.</p>
<h5><strong>3. SelectScience/Beckman Coulter: Advancing EV research – First Experiences with the CytoFLEX Nano Flow Cytometer</strong></h5>
<ul>
<li>Tu, 30.7.2024 17:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4109">Registration</a></li>
</ul>
<p>We will discuss the advantages of using fluorescence combined with multiple scatters for further characterization of nanoparticles and extracellular vesicles.</p>
<h5><strong>4. Agilent Technologies: Simplified Measurement of Content Distribution of Pharma Tablets</strong></h5>
<ul>
<li>Tu, 30.7.2024 21:30 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/3905">Registration</a></li>
</ul>
<p>Content distribution of entire pharma tablets can be quickly measured using the component-specific workflow of the LDIR.</p>
<h5><strong>5. Technology Networks/Refeyn: Enhancing the Development of AAV-Based Therapeutics With Mass Photometry</strong></h5>
<ul>
<li>We, 31.7.2024 17:00 CEST</li>
<li><a href="https://icpms.labrulez.com/webinar/4180">Registration</a></li>
</ul>
<p>Enter mass photometry, a new bioanalytical technique that can efficiently characterize AAVs and distinguish between full and empty AAVs.</p>


Webinars LabRulezICPMS Week 31/2024

<p><strong>Although he originally wanted to study mathematics, Tomáš Janoš eventually became an environmental epidemiologist. He completed his doctorate at RECETOX focusing on human biomonitoring, for which he received the Dean’s Award of the MU Faculty of Science. When he was in Spain, analyzing mortality due to heat and cold, he realized that he wanted to devote himself to studying the impact of extreme temperatures on human health. In the interview, he explains the origins of his interest in climate change, how such a topic is communicated to the public, and why he believes it is crucial for scientists in the Czech Republic to focus more on this issue.</strong></p>
<h5><strong>How did you become interested in climate change in your scientific work?</strong></h5>
<p>I was interested in climate change and the impacts of extreme temperatures on human health for most of my doctorate but I found space for this topic only at the very end. I noticed that in southern European countries, research on the impact of temperature on health is advancing, while Central and Eastern Europe is lagging. So, I thought exploring whether high and low temperatures have similar effects here would be interesting. At that time, I contacted colleagues from the Institute for Global Health in Barcelona, who have extensive experience with research on this topic. Then, I went to Spain with Czech data to learn how to process and analyze them. The topic intrigued me during my three months there, so I decided to write my own project and focus more on this direction.</p>
<h5><strong>In October 2023, a study on the relationship between mortality rates and extreme temperatures was published based on your work. Are you still dealing with this topic?</strong></h5>
<p>Yes, in connection with extreme temperatures, I also started to examine ambulance dispatches in the Czech Republic, which have not yet been explored on the national level in Europe. Such research examines different socio-economic groups and locations, which is important because temperatures vary from place to place. We have written a scientific article on this topic, which we hope to publish by the end of the year. Interestingly, not just elderly people, but also small children, teenagers, and young adults are at high risk. I also have a project studying the impact of short-term temperature fluctuations in combination with polluted air, such as smog, on the number of work accidents, especially among outdoor workers. I will be doing research for another two years, again with people from Barcelona, who are trying to create a European-wide database of work accidents, which already includes the Czech Republic, among others.</p>
<h5><strong>Is it difficult to get data for this type of research?</strong></h5>
<p>Data on deaths is obtained through excellent cooperation with the Institute of Health Information and Statistics of the Czech Republic. On the other hand, data on ambulance dispatches are much more difficult to obtain because of their sensitivity. We get them for certain territorial units, ambulance dispatches for example for districts, which is a terribly large territorial unit. If we went lower, we would get to the numbers of one, two dispatches a day, from which it would already be possible to deduce who it was specifically on a smaller territory. Also, not in all countries have data on ambulance dispatches collected and a database is made. Here, the information is again collected by the Institute of Health Information and Statistics of the Czech Republic from insurance companies, so a summary can be made. However, not in all countries does it work so comprehensively.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/RECETOX_In_the_next_years_climate_change_will_affect_everything_says_environmental_epidemiologist_Tomas_Janos_1_53337bc013.jpg" alt="RECETOX: In the next years, climate change will affect everything, says environmental epidemiologist Tomáš Janoš 1."/></p>
<h5><strong>So you work more with a summary of data, how do you find out that the dispatches were related to the temperature?</strong></h5>
<p>In general, attributing something to the weather is extremely difficult, something like that is not often even part of the diagnosis. Then there are epidemiological models that calculate with daily temperature and dispatches, and when they see some fluctuation that cannot be explained by anything else, they attribute it to the temperature. So we just get specific numbers and we do the calculations through models.</p>
<h5><strong>As for extreme temperatures in cities in general, can the aforementioned health risks be systematically prevented?</strong></h5>
<p>Definitely. Mortality studies for example show that in greener cities it is generally cooler by one to two degrees, which can save several lives a year. Furthermore, a network of fountains can be built, where a person can cool down. In Barcelona, for example, they have so-called climate refuges. It is a network of places where people from can hide in case of a heat wave. It’s nothing special, they are usually air-conditioned public places like supermarkets, libraries or museums, for which a map is created. Thanks to it, people find out where they can go in case of need and where they will be accepted. Therefore, it is important to inform the public and communicate the existence of refuges well, especially to populations which are at a higher risk, such as the elderly, and lower socioeconomic groups, for example by distributing leaflets. In Spain, they also created an early warning system, which several days in advance reports how high is the probability that due to high temperatures a certain number of people will die. Although it sounds morbid, it allows public institutions, such as hospitals and rescue services, to prepare for such a risk.</p>
<h5><strong>As for communication with the public, do you cooperate with other organizations or other public actors?</strong></h5>
<p>As an expert, I cooperate externally for example with Klimatická koalice, which is a platform of Czech non-profit organizations dealing with climate protection. One of their recent activities is the education of medical students in the field of climate change and how it can affect human health, because it is completely missing in the curriculum. The idea is that older people, for whom climate change poses the highest risk in terms of health, but at the same time tend to underestimate it the most, could draw information from doctors, who are a trustworthy authority for them.</p>
<h5><strong>And what does such cooperation bring to you as a scientist?</strong></h5>
<p>From my point of view as a scientist, it is often difficult to get the information to the right place where it will have an effect. Organizations like Klimatická koalice, Fakta o klimatu or the Institute for Czech Policy EUROPEUM help me a lot in this. When we published the study, thanks to the fact that I sent the information about it to everyone I knew would be interested in it, several public or media outputs were created. I probably wouldn’t be able to get it into awareness like this myself.</p>
<h5><strong>So do you have to initiate the first dissemination of information yourself?</strong></h5>
<p>Yes, we as scientists are not pushed into popularizing science much. It is usually more our own interest and it is not part of our performance. Communication is important and should be taken care of. In science, we often pat ourselves on the back, what a great article we published, but when you come to ordinary people outside the scientific sphere, they are interested in information, not the impact of the journal. Of course, at the beginning there must be a quality scientific study, but the actual effect should not be forgotten. Another thing is that it is difficult to communicate a complex problem well and convey it in a few sentences. To do it well, you need to educate yourself. But in practice, there is no time to do either.</p>
<h5><strong>What is it like to communicate the topic of climate change from the perspective of science?</strong></h5>
<p>There is definitely interest in the climate among the public, but when you say climate, it acts like a red rag. It evokes in people things that they will not be able to do or something for which others will condemn them. Moreover, I am afraid that over time it will rather worsen. At the same time, I think that people are interested in climate protection and the environment, we are just at it again, that it is necessary to communicate it all better.</p>
<h5><strong>How does the scientific community react to the topic of climate change? Is there interest there too?</strong></h5>
<p>I feel that the interest is primarily abroad so far. The impacts of it probably do not affect the Czech Republic so much yet, but it certainly awaits us. In the next fifty years, climate change will affect everything, including environmental epidemiology. I am dealing with a very limited section of the whole issue, but there are many more aspects. Through new invasive species, it will be necessary to change the way pesticides are used, there will be an increase in the incidence of infectious diseases, floods, cloudbursts, the change will affect air research, food safety or migration. We should not close our eyes to such a complex and difficult to explain problem as climate change. Whole research groups are devoted to it at foreign institutes, so the Czech Republic should not miss the boat.</p>


In the next years, climate change will affect everything, says environmental epidemiologist Tomáš Janoš.

<h3><strong>About us</strong></h3>
<p>Our laboratory is equipped with high-end mass spectrometry instruments, which offers services in field of <strong>PROTEOMICS</strong> and <strong>METABOLOMICS</strong>. All our services are available for everybody from the academic and non-academic areas.</p>
<h5><a href="https://www.biocev.eu/en/services/omics-mass-spectrometry-core-facility.6#core-facility-services"><strong>Our Services:</strong></a></h5>
<ul>
<li><strong>Preparation of samples</strong> for analysis (cells, body fluids, tissues, plants etc.)</li>
<li><strong>Targeted</strong> or <strong>untargeted</strong> <strong>proteomics</strong> and <strong>metabolomics</strong> analysis</li>
<li><strong>Data analysis</strong> and basic statistical evaluation</li>
<li><strong>Consultation</strong> of experimental design and the results</li>
</ul>
<h5><strong>Proteomics:</strong></h5>
<p>We are modern well equipped laboratory with the technical background able to perform most of the currently used <a href="https://www.biocev.eu/en/services/omics-mass-spectrometry-core-facility.6/untargeted-proteomics.24?t=s"><strong>untargeted</strong></a> and <a href="https://www.biocev.eu/en/services/omics-mass-spectrometry-core-facility.6/targeted-proteomics.25?t=s"><strong>targeted</strong></a> proteomic measurements. We provide full proteomics service, including protein isolation from the supplied sample to evaluation and consultation of the measured data. Since 2015, we handle approximately 300 orders per year (roughly 3000 samples). Results based on measurements from our laboratory were used in dozens of publications. To consult your plan, please contact Karel Harant (karel.harant@natur.cuni.cz) or Pavla Talacko (pavel.talacko@natur.cuni.cz).</p>
<h5><strong>Metabolomics:</strong></h5>
<p>As a service, we provide <a href="https://www.biocev.eu/en/services/omics-mass-spectrometry-core-facility.6/targeted-metabolomics.26?t=s"><strong>targeted</strong></a> and <a href="https://www.biocev.eu/en/services/omics-mass-spectrometry-core-facility.6/untargeted-metabolomics.30?t=s"><strong>untargeted</strong></a> analysis of small molecules. Thanks to the combination of equipment for <strong>LC-MS</strong> and <strong>GCxGC-MS</strong>, it is possible to measure a wide range of small molecules. We use several types of <strong>C18</strong> and <strong>HILIC</strong> stationary phase columns on LC. For GC, we have various columns for different types of analytes. Individual measuremnts must always be consulted in advance, an individual approach and method must be chosen for each group of molecules. Please contact Olga Součková (souckoo@natur.cuni.cz) for LC-MS or Petr Žáček (zacek@natur.cuni.cz) for GCxGC-MS.</p>
<blockquote>
<p><strong>All our services are available for everybody from the academic and non-academic areas.</strong></p>
</blockquote>
<blockquote>
<p><strong>Contact us before you start your first experiment: +420 325 873 925!</strong></p>
</blockquote>
<blockquote>
<p><strong>23.7.2024 Waiting time from “sample to result” is approximately 6-8 weeks.</strong></p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/BIOCEV_Introducing_BIOCEV_OMICS_Mass_Spectrometry_Core_Facility_cover_image_7cb6de4462.jpg" alt="BIOCEV: BIOCEV OMICS Mass Spectrometry Core Facility"/></p>
<h3><strong>Services</strong></h3>
<ul>
<li>Preparation of samples for analysis</li>
<li>Untargeted and targeted proteomics</li>
<li>Untargeted and targeted metabolomics</li>
<li>Data analysis and basic statistical processing</li>
<li>Consultation of experiment design and measured data</li>
</ul>
<h4><strong>Untargeted proteomics</strong></h4>
<h5><strong>Up to 8000 identified proteins in one injection.</strong></h5>
<p>A basic type of proteomic experiment. Complete lysate is digested with trypsin. The resulting peptides are desalted and separated on a reverse phase column. Due to the low amount of proteins and the high complexity of the sample, liquid chromatography is currently most often used in nanoflow - hundreds of nanoliters per minute. Liquid chromatography is directly connected to the electrospray of the Orbitrap Fusion or Orbitrap Ascend mass spectrometer. The mass spectrometer measures alternately in two modes: MS¹, where peptide masses are measured in the Orbitrap with high resolution and accuracy, and MS², in which peptides selected from the previous MS¹ scan are fragmented. These are measured either again in the Orbitrap or in the ion trap. The ion trap provides lower accuracy and resolution than the Orbitrap, but is faster and more sensitive.</p>
<blockquote>
<p><strong>We offer untargeted proteomics measurement and analysis of post-translational modifications in different types of samples (cells, tissues, body fluids, plant samples, etc.).</strong></p>
</blockquote>
<blockquote>
<p><strong>Our newest service is &quot;Thermal proteome profiling (TPP)&quot; that allows you direct detection of drug-protein interactions in living cells.</strong></p>
</blockquote>
<h5><strong>Examples of analyses:</strong></h5>
<ul>
<li>LFQ (label free quantification)</li>
<li>TMT (tandem mass tags)</li>
<li>TPP (Thermal proteome profiling)</li>
<li>phosphoproteome profiling</li>
<li>pull-down experiments</li>
</ul>
<h5><strong>Tips for sample handling:</strong></h5>
<ul>
<li>do not use trypsin for cell harvesting</li>
<li>inform us about presence of detergent</li>
<li>always keep in mind same protein loading for pull-down experiments (eg. Co-immunoprecipitation)</li>
<li>do not use phosphatase inhibitors for phosphoproteomic experiments</li>
</ul>
<h4><strong>Targeted proteomics</strong></h4>
<h5><strong>Accurate quantification of selected proteins.</strong></h5>
<p>Method aims to validate the results from an untargeted approach. It is performed on a triple quadrupole in SRM mode or using the MRM method on an Orbitrap. It is an an alternative approach to common techniques such as western blot. The advantage of this approach is the possibility to analyze several proteins (dozens) at once during one injection. This eliminates the problem of finding suitable antibodies.</p>
<blockquote>
<p><strong>The targeted proteomics service is always project specific and we offer it after prior meeting with the customer.</strong></p>
</blockquote>
<p>Parallel analysis of peptides labeled with heavy amino acids is essential. These serve as an internal standards.</p>
<p>The amount of proteins that can be targeted in one injection using a triple quadrupole, is strongly dependent on the concentration of the target proteins in the sample. The lower the expression of the target proteins, the less it is possible to analyze them in one injection due to the need for higher loading times. Information of fragmentation properties of targeted peptides is also important. This is usually obtained from an untargeted experiment, which should precede the targeted proteomic analysis. If the protein of interest was not detected during an untargeted experiment, then its targeted analysis is very difficult. However, in some cases fragmentation information can be obtained from publicly available databases.</p>
<h5><strong>Tips for sample handling:</strong></h5>
<ul>
<li>do not use trypsin for cell harvesting</li>
<li>inform us about presence of detergent</li>
<li>always keep in mind same protein loading for pull-down experiments (eg. Co-immunoprecipitation)</li>
<li>do not use phosphatase inhibitors for phosphoproteomic experiments</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/BIOCEV_Introducing_BIOCEV_OMICS_Mass_Spectrometry_Core_Facility_Targeted_proteomics_1_f0605d8efc.jpg" alt="BIOCEV: Introducing BIOCEV OMICS Mass Spectrometry Core Facility - Targeted proteomics."/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/BIOCEV_Introducing_BIOCEV_OMICS_Mass_Spectrometry_Core_Facility_Targeted_proteomics_2_f1af260d27.jpg" alt="BIOCEV: Introducing BIOCEV OMICS Mass Spectrometry Core Facility - Targeted proteomics."/></p>
<h4><strong>Untargeted metabolomics</strong></h4>
<p>The goal of untargeted metabolomics is to cover the widest possible spectrum of metabolites present in the sample, their identification, and quantification. The development of this approach was made possible by the recent advances in instrumentation and computing. Our laboratory equipment enables non-targeted analyses by liquid and gas chromatography.</p>
<blockquote>
<p><strong>We offer untargeted analyses of metabolites in various biological matrices and basic statistical evaluation of the data (Figure 2, 3, 4).</strong></p>
</blockquote>
<blockquote>
<p><strong>Our newest service is stable isotope tracing (Figure 1).</strong></p>
</blockquote>
<p>HPLC-MS/MS (Figure 2): Analyzes are performed on Thermo Scientific equipment on an Ultimate 3000 liquid chromatograph coupled to an Orbitrap Fusion mass detector. The measured data are evaluated by the Compound Discoverer software.</p>
<p>GCxGC-MS (Figure 3): Analyzes are performed on a two-dimensional comprehensive chromatograph connected to a Pegasus 4D mass detector. We evaluate the data in ChromaTOF v4.5 software.</p>
<p>The liquid chromatography method covers non-volatile substances, generally polar, soluble in water, and polar organic solvents (methanol, acetonitrile...). The gas chromatography method is more suitable for analyzing stable volatile non-polar substances up to a weight of approx. 1000 Da. The range of measured substances can be increased for both methods eg., by chemical modifications (derivatization) of the analyzed molecules, which adjust their polarity and volatility (for GC) and/or stability (for LC). We choose a suitable technique or a combination of several techniques based on prior consultation with the customer.</p>
<p>The untargeted analysis results in a list of substances found in the samples, a proposal of probable structures, and an indication of the quantity of individual compounds. We also provide a basic statistical evaluation focusing on looking for differences between the tested sample groups (Figure 4).</p>
<h5><strong>Tips for sample handling:</strong></h5>
<ul>
<li>do not use phosphate buffers in the sample</li>
<li>inform us about sample type, growth medium, chemicals used, etc.</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/BIOCEV_Introducing_BIOCEV_OMICS_Mass_Spectrometry_Core_Facility_Untargeted_metabolomics_Fig_1_11750e6992.jpg" alt="BIOCEV: Introducing BIOCEV OMICS Mass Spectrometry Core Facility - Untargeted metabolomics Fig. 1."/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/BIOCEV_Introducing_BIOCEV_OMICS_Mass_Spectrometry_Core_Facility_Untargeted_metabolomics_Fig_2_5ca1521705.jpg" alt="BIOCEV: Introducing BIOCEV OMICS Mass Spectrometry Core Facility - Untargeted metabolomics Fig. 2."/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/BIOCEV_Introducing_BIOCEV_OMICS_Mass_Spectrometry_Core_Facility_Untargeted_metabolomics_Fig_3_f4f1050fc5.jpg" alt="BIOCEV: Introducing BIOCEV OMICS Mass Spectrometry Core Facility - Untargeted metabolomics Fig. 3."/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/BIOCEV_Introducing_BIOCEV_OMICS_Mass_Spectrometry_Core_Facility_Untargeted_metabolomics_Fig_4_b3e198dbc9.jpg" alt="BIOCEV: Introducing BIOCEV OMICS Mass Spectrometry Core Facility - Untargeted metabolomics Fig. 4."/></p>
<h4><strong>Targeted metabolomics</strong></h4>
<p>Targeted metabolomics includes analyses of selected metabolites, which are then detected selectively and with greater sensitivity than applying an untargeted approach. It is also possible to quantify substances both relatively and absolutely. In the case of absolute quantification, it is necessary to supply a suitable labeled analog of the determined substance.</p>
<blockquote>
<p><strong>We offer analysis of primary cellular metabolites - see List of substances.</strong></p>
</blockquote>
<blockquote>
<p><strong>We analyze metabolites in tissues, cell pellets, incubation media, and body fluids (plasma, urine...).</strong></p>
</blockquote>
<blockquote>
<p><strong>We also offer the possibility of developing a method for the analysis of substances that are not in the attached list or for other matrices.</strong></p>
</blockquote>
<p>The attached list contains substances with the analysis of which we have experience. We adapt each method to the type of experiment and matrix. We can develop a method for any substance or group of substances capable of being detected by liquid and gas chromatography techniques (both connected to a mass detector or a UV detector in liquid chromatography). In the case of the development of a new method, it is necessary to supply the standards of these substances.</p>
<h5><strong>Tips for sample handling:</strong></h5>
<ul>
<li>do not use phosphate buffers in the sample</li>
<li>inform us about sample type, growth medium, chemicals used, etc.</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/BIOCEV_Introducing_BIOCEV_OMICS_Mass_Spectrometry_Core_Facility_Targeted_metabolomics_List_of_substances_a6a4198b34.jpg" alt="BIOCEV: Introducing BIOCEV OMICS Mass Spectrometry Core Facility - Targeted metabolomics: List of substances."/></p>
<h3><a href="https://www.biocev.eu/en/services/omics-mass-spectrometry-core-facility.6#core-facility-equipment"><strong>Equipment</strong></a></h3>
<h4><strong>Mass spectrometers</strong></h4>
<h5><strong>Orbitrap Ascend</strong></h5>
<ul>
<li><a href="https://lcms.labrulez.com/library?instrumentationItems=31-32-33-30&amp;mainauthorItems=3&amp;search=Ascend"><strong>Application in the LabRulezLCMS library</strong></a></li>
</ul>
<p>Thermo Scientific Orbitrap Ascend Tribrid mass spectrometer, offering new capabilities for multiplexed proteomics and native protein characterization. The mass spectrometer enables improved sample throughput, versatility and ease-of-use — producing high quality data with simplicity. More samples can be analyzed at lower concentrations, enabling faster, more sensitive analysis, and macromolecule experiments, that were not possible before, can now be achieved. The new Auto-Ready ion source for calibration maximizes convenience, allowing the user to spend more time on the results, and less time on instrument set-up.</p>
<h5><strong>Orbitrap Fusion</strong></h5>
<ul>
<li><a href="https://lcms.labrulez.com/library?instrumentationItems=31-32-33-30&amp;mainauthorItems=3&amp;search=Fusion"><strong>Application in the LabRulezLCMS library</strong></a></li>
</ul>
<p>Mass spectrometer combining Quadrupole, Ion trap and Orbitrap. The quadrupole is used as a mass filter for the selection of precursors, after which the instrument branches and the ions can travel either to the Orbitrap - a very accurate and high-resolution mass analyzer that uses the Fourier transform to determine their mass, or to the collision cell, where they can be fragmented. From there, the path leads either back to the Orbitrap or further to the linear ion trap. This serves as the second mass analyzer in the device. It is less accurate and low-resolution, but provides greater sensitivity and the possibility of ion fragmentation. Fragmentation products can be isolated in the trap and fragmented again. This enables analysis in MS³ and even higher levels of fragmentation.</p>
<h5><strong>TSQ Quantiva Triple Quadrupole Mass Spectrometer</strong></h5>
<ul>
<li><a href="https://lcms.labrulez.com/library?instrumentationItems=31-32-35&amp;mainauthorItems=3&amp;search=Quantiva"><strong>Application in the LabRulezLCMS library</strong></a></li>
</ul>
<h5><strong>Leco - Pegasus® 4D GCxGC-TOFMS</strong></h5>
<ul>
<li><a href="https://gcms.labrulez.com/library?instrumentationItems=3-13-2&amp;mainauthorItems=24&amp;search=4D"><strong>Application in the LabRulezGCMS library</strong></a></li>
</ul>
<h4><strong>Liquid chromatography</strong></h4>
<ul>
<li>nano LC Thermo Dionex Ultimate 3000</li>
<li>LC Thermo Dionex Ultimate 3000</li>
</ul>
<h4><strong>Sample preparation</strong></h4>
<ul>
<li>KingFisher Flex</li>
<li>vacuum concentrator</li>
<li>Low flow fraction collector - Spider</li>
<li>Bioruptor pico - sonication bath</li>
</ul>
<h4><strong>Software</strong></h4>
<ul>
<li>Spectronaut 19</li>
<li>FreeStyle</li>
<li>ChromaTOF 4.5</li>
<li>Proteome Discoverer 2.2</li>
<li>Scaffold Q+S 4.6.1</li>
<li>Compound Discoverer 3.3</li>
<li>TraceFinder</li>
</ul>
<h5><strong>Software - Freeware</strong></h5>
<ul>
<li>LipidHunter</li>
<li>MaxQuant</li>
<li>Perseus</li>
<li>Skyline</li>
</ul>
<h3><a href="https://www.biocev.eu/en/services/omics-mass-spectrometry-core-facility.6#core-facility-team"><strong>Team</strong></a></h3>
<h5><strong>Mgr. Karel Harant</strong></h5>
<ul>
<li>Head of Proteomic Core Facilty</li>
</ul>
<h5><strong>Mgr. Pavel Talacko</strong></h5>
<ul>
<li>Non-targeted proteomics, targeted proteomics</li>
</ul>
<h5><strong>RNDr. Petr Žáček, Ph.D.</strong></h5>
<ul>
<li>GC / MS specialist, small molecule analysis</li>
</ul>
<h5><strong>Mgr. Veronika Ševců</strong></h5>
<ul>
<li>researcher, preparation of proteomic samples</li>
</ul>
<h5><strong>RNDr. Petr Žáček, Ph.D.</strong></h5>
<h5><strong>Mgr. Veronika Ševců</strong></h5>
<ul>
<li>researcher, preparation of proteomic samples</li>
</ul>
<h5><strong>Ing. Olga Součková, Ph.D.</strong></h5>
<ul>
<li>LC/MS specialist, small molecule analysis</li>
</ul>


Introducing BIOCEV OMICS Mass Spectrometry Core Facility

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RECETOX (Research Centre for Toxic Compounds in the Environment) is an independent department of the Faculty of Science of Masaryk University in Brno, which has been established in 1983. RECETOX is focused on the research and education in cross-cutting area of Environment and Health. It addresses interactions among chemicals, environment, and biological systems, including consequences at local, regional, and global levels.

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