NIST26 includes integrated processing tools for LC-MS/MS data, but there are situations where working directly from instrument vendor software can be faster and more practical. When only a few peaks need to be evaluated, when mzML conversion is inconvenient, or when nominal-mass data are involved, spectra can be transferred directly into NIST Library Search for identification. This workflow can streamline spectral searching while still providing access to NIST high-resolution and low-resolution libraries, public MS/MS resources, and flexible search settings.<h4>When does direct transfer from vendor software make sense?</h4>NIST26 provides integrated data processing in the Chromatogram tab, including workflows for deconvolution and library searching. In many cases, this is the most comprehensive way to process LC-MS/MS datasets.However, chromatogram-based processing generally requires conversion of the original vendor data into mzML, for example using ProteoWizard/MSConvert. If a chromatogram is relatively simple, deconvolution is unnecessary, and only a small number of peaks need to be investigated, converting and processing the entire file may add unnecessary steps.In these situations, it can be more efficient to select an MS/MS spectrum directly in the original vendor software and send it to NIST Library Search.There are also other cases where direct transfer becomes particularly useful. Some proprietary data formats may not convert cleanly into mzML, while nominal-mass MS/MS data are not supported for MS/MS searching in the NIST26 Chromatogram tab. For this type of data, transferring or importing spectra into Library Search becomes the practical alternative.<h4>Which vendor software can send spectra directly to NIST?</h4>Several major instrument platforms provide direct integration with NIST Library Search.The workflow is available with software from:<ul><li>Agilent, including MassHunter and OpenLab,</li><li>Waters,</li><li>Thermo Fisher Scientific.</li></ul>Wiley KnowItAll can also import spectra into NIST Search and supports numerous vendor data formats through its own software environment.For some other platforms, direct NIST integration may not be available. In those situations, spectra can potentially be exported in text-based formats such as TXT or MSP ASCII and then imported into the Spectrum List in NIST Library Search.Direct transfer is generally more convenient because it avoids intermediate export and import steps.<h4>A practical Agilent example</h4>The workflow can be demonstrated using Agilent software.An analyst first selects the desired MS/MS region, averages the spectrum if necessary, and transfers it into the spectrum display. From there, the spectrum can be sent to NIST using the Search Using NIST MS Program command.Once properly configured, NIST automatically receives the spectrum and initiates the specified search. Before relying on this workflow, however, the import and library search parameters should be checked carefully.<h4>Setting up accurate-mass spectrum import</h4>For high-resolution MS/MS data, the first important step is found under Spectrum Import Options.Accurate-mass spectra should be imported with the appropriate number of decimal places for precursor and product ions, and the Accurate m/z spectrum type must be selected.Preserving mass accuracy is essential because one of the main advantages of HRMS library searching is the ability to distinguish candidates using precise precursor and fragment masses.Once the import settings are correct, the next step is configuring the library-search parameters.<h4>Searching without a precursor ion</h4>Depending on how the spectrum is selected or averaged in the vendor software, precursor information may not always be transferred automatically.NIST26 can still search such a spectrum using HiRes NoPrecursor mode.Alternatively, if the precursor m/z is known, it can be entered manually and the search can then include precursor information.The no-precursor approach can be convenient when quickly screening manually selected MS/MS spectra, particularly when the fragment pattern itself contains enough information to generate useful candidate matches.<h4>Setting mass tolerances</h4>For accurate-mass MS/MS searching, suitable precursor and product-ion tolerances are essential.An example configuration uses:<ul><li>20 ppm for the precursor ion</li><li>40 ppm for product ions</li></ul>These settings can be kept consistent with those used in the NIST26 Chromatogram workflow.Proper tolerance settings help balance search sensitivity with selectivity. If tolerances are too wide, more irrelevant candidates may be returned; if they are too narrow, valid matches can be missed because of normal measurement variation.<h4>Combining multiple spectral libraries</h4>One of the strengths of NIST Search is the ability to search multiple libraries simultaneously.A typical configuration can include the NIST high-resolution MS/MS library together with the NIST low-resolution tandem library.The NIST low-resolution library can participate in accurate-mass workflows because it contains the required indexing information. This is important because not every custom or older low-resolution library will necessarily be compatible with the same type of high-resolution search.Additional public resources can also be included, such as:<ul><li>MS-DIAL positive-mode libraries</li><li>MS-DIAL negative-mode libraries</li><li>MoNA LC-MS/MS</li></ul>Combining commercial and public libraries can substantially broaden spectral coverage. In the demonstrated workflow, several million spectra could be searched in a single configuration.For laboratories dealing with unusual metabolites, impurities, environmental contaminants, or compounds outside mainstream commercial libraries, this expanded search space can be particularly valuable.<h4>Structure searches require separate library selection</h4>MS/MS searching and structure searching do not necessarily use the same library selections.If structural searching is required, the appropriate structure-containing libraries must also be loaded in the corresponding Library Search settings.This distinction is easy to overlook when first configuring NIST26. A library may contain useful spectra but no associated structures, or the structure database may need to be explicitly selected before substructure or related structural searches can be performed.<h4>Automating the search</h4>Once the library and tolerance settings have been defined, Automatic Search can be enabled.This allows the spectrum transferred from vendor software to trigger a search immediately rather than requiring the user to launch it manually each time.For repeated workflows involving many manually selected peaks, this small automation can save considerable time.After the configuration has been optimized, it is useful to save it as a dedicated setup—for example, a configuration specifically designed for vendor-imported spectra.<h4>Understanding the result table</h4>NIST search results are typically ranked by score, but additional parameters can also help interpret the matches.Fields can include:<ul><li>score,</li><li>reverse score,</li><li>partial spectrum search score,</li><li>dot product,</li><li>molecular weight,</li><li>other user-selected parameters.</li></ul>The search result display can be customized by right-clicking the column headers and adding or removing fields.Although the standard score will usually remain the primary ranking criterion, alternative metrics can be useful when evaluating spectra with missing fragments, additional peaks, partial spectral overlap, or related structures.<h4>Why it can be useful to keep duplicate matches visible</h4>NIST includes a Best Matching Only option that reduces duplicate or repeated entries in the displayed results.When enabled, the software may reduce a list containing several library records of the same compound to the best representative match.This makes the result list easier to review, but it can also hide useful information.For initial evaluation, it is often preferable to leave this option disabled so that all matching spectra remain visible. Multiple spectra of the same compound may come from different collision energies, instruments, ion forms, or libraries, and reviewing these variations can provide useful confirmation.Filters can always be applied later once the analyst understands the full result set.<h4>Visual comparison of unknown and library spectra</h4>NIST Library Search provides several complementary views of each candidate.The experimentally acquired unknown spectrum is displayed together with the selected library spectrum, while a head-to-head spectral comparison provides an immediate visual representation of matching and non-matching ions.This is important because library-search scores should not be treated as a fully automated identification decision.A strong match should still be examined visually, especially when dealing with complex spectra, low-abundance fragments, co-isolated ions, or compounds with closely related structural analogues.The combination of numerical scoring and visual inspection provides a stronger basis for interpretation.<h4>An important issue with the Intensity Threshold setting</h4>One practical software issue deserves particular attention when importing high-resolution spectra.The Spectrum Import Options window includes an Intensity Threshold function that can remove low-intensity signals and reduce spectral noise.In principle, this would be useful. However, when an intensity threshold is applied, imported high-resolution spectra may lose their decimal mass information and be transferred as nominal-mass spectra instead.An accurate-mass peak such as 176.0815 could therefore appear simply as m/z 176.For HRMS users, this effectively removes one of the most important dimensions of the data and can compromise accurate-mass searching.Until this behavior is corrected, the safer approach is to avoid using the Intensity Threshold option during high-resolution spectrum import and perform any necessary filtering elsewhere.<h4>A related display issue in the Chromatogram window</h4>A second software behavior concerns how Spectrum Import Options are reflected in the Chromatogram tab.If the decimal precision is set to zero for importing low-resolution spectra, the head-to-head spectral display may subsequently show masses truncated according to those settings.Importantly, this is a display issue only.It does not change the accurate-mass deconvolution results or the library-search table. The underlying processed data remain unaffected; only the visual comparison at the top of the Chromatogram window reflects the altered import settings.Understanding this distinction can prevent users from mistakenly assuming that accurate-mass data have been lost from the processed results.<h4>When direct vendor-to-NIST searching is most useful</h4>Direct transfer from vendor software is not intended to replace the complete NIST26 Chromatogram workflow.Instead, it provides a complementary route for situations where full chromatographic processing is unnecessary or unavailable.It can be particularly effective when:<ul><li>only a few peaks need identification,</li><li>deconvolution is not required,</li><li>mzML conversion adds unnecessary processing,</li><li>vendor files cannot be converted reliably,</li><li>nominal-mass MS/MS spectra need to be searched,</li><li>spectra have already been reviewed and selected in the vendor environment.</li></ul>For these cases, direct spectrum transfer offers a fast route from the original MS/MS data to spectral library identification.<h4>Connecting vendor software with broader NIST26 capabilities</h4>The workflow also illustrates a broader advantage of NIST26: analysts do not have to choose between the instrument vendor's software and an independent spectral-search environment.Vendor software can remain the primary platform for inspecting chromatograms and selecting spectra, while NIST provides access to large commercial libraries, public MS/MS databases, flexible scoring, spectral comparison, and additional interpretation tools.Used together, these environments can provide a practical workflow for both rapid routine searching and more detailed unknown identification.The key is careful configuration. Accurate-mass import settings, mass tolerances, library selection, search mode, and result-display options all influence how effectively the workflow performs.Once these parameters are properly established and saved, sending a spectrum from the vendor software to NIST26 can become a rapid and repeatable part of everyday LC-MS/MS data interpretation.

Sending MS/MS (Tandem) Spectra to NIST26 Search

Compositional and Metabolomic Characterization of Major On-Farm Side Streams and Derived Products of Industrial Fine-Flavor Cocoa Processing

The integrity of sport depends on the ability to detect prohibited substances accurately, reliably and with confidence. However, as doping strategies become increasingly sophisticated, the analytical demands placed on anti-doping laboratories continue to evolve.Today’s laboratories must do more than test for a static list of known compounds. They need broad analytical strategies capable of addressing hundreds of substances across multiple chemical classes, identifying emerging threats and supporting defensible confirmation and reporting of adverse analytical findings. For more than 25 years, Thermo Fisher Scientific has worked globally with World Anti-Doping Agency (WADA)-accredited laboratories to advance the analytical science supporting modern anti-doping programs.<h3>Anti-doping testing is a moving target</h3>The World Anti-Doping Agency publishes an annually updated Prohibited List that provides a global framework for testing programs. Yet the list is non-exhaustive, meaning laboratories may also need to detect structurally related compounds and substances across several categories, with similar chemical structure and biological activity. That creates a significant analytical challenge. Anti-doping laboratories must be prepared to monitor a broad range of compounds while maintaining the sensitivity, specificity, and confidence required for both initial testing and confirmation. At the same time, the landscape continues to change. Emerging substances, rapidly expanding doping strategies and new approaches to sample collection and analysis are creating multiple considerations for anti-doping laboratories to consider. Dried blood spots (DBS), for example, have gained attention as a complementary approach to traditional urine and venous blood collection supporting applications such as detecting steroid esters and monitoring peptides. High-resolution mass spectrometry is also enabling laboratories to consider both targeted analysis and retrospective interrogation of previously acquired data as scientific knowledge and regulations evolve.<h3>From screening to confident confirmation</h3>No single analytical technique can address every anti-doping challenge.With respect to chemical analysis of toxicologically relevant compounds, modern doping control laboratories employ complementary technologies—including liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS) and isotope ratio mass spectrometry (IRMS)—depending on the compounds and analytical questions involved.For initial testing, laboratories may need to efficiently screen biological samples for numerous prohibited substances spanning categories such as steroids, stimulants, metabolic modulators, diuretics, narcotics, cannabinoids and peptides.When &nbsp;an initial Testing Procedure produces a result requiring confirmation, laboratories need analytical approaches capable of supporting confident confirmation.This combination of broad screening and targeted confirmation has made mass spectrometry central to modern anti-doping science.<h3>Expanding possibilities with LC-MS</h3>High-resolution accurate-mass mass spectrometry can provide precise mass measurements while supporting comprehensive screening of known and suspected substances, while preserving information-rich data that can support investigation of emerging or previously untargeted compounds.An important advantage of high-resolution mass spectrometry is the ability to revisit previously acquired data. As new substances, metabolites or other analytical targets are identified, laboratories can potentially re-process previously acquired full scan data (and any corresponding MSn experiment) without reanalyzing the original sample. This strategy is currently used by laboratories to support global re-testing programs.&nbsp; Triple quadrupole <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?category=20&amp;manufacturerItems=3">LC-MS</a> can complement these workflows by supporting sensitive, targeted quantitation and confirmation of prohibited substances.Together, these approaches provide laboratories the flexibility needed to address today’s testing requirements while giving laboratories greater flexibility to respond to emerging analytical challenges.<h3>The continuing role of GC-MS/MS</h3>Gas chromatography coupled with triple quadrupole mass spectrometry remains an important technology in anti-doing laboratories, particularly for urinary steroid profiling and the screening and confirmation of anabolic- &nbsp;androgenic steroids and other GC-amenable prohibited substances.GC-MS/MS can support both screening and confirmation while providing the sensitivity, selectivity and dymanic range required for screening and confirmatory analysis.The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/485">Thermo Scientific TSQ 9610 Triple Quadrupole GC-MS</a> is designed to maximize sample throughput, helping laboratories address the demands of routine testing while maintaining analytical performance across a wide dynamic range. &nbsp;The TSQ 9610 incorporates NeverVent technology, designed to increase instrument uptime by reducing the disruption associated with routine source maintenance.As anti-doping workflows evolve, bridging screening and confirmation together within robust analytical strategies can help laboratories respond efficiently to increasingly complex testing requirements.<h3>When identifying a steroid isn’t enough</h3>In some anti-doping investigations, simply detecting a compound does not provide the complete answer.Testosterone, for example, can be produced naturally by the body or administered externally as an anabolic steroid. In cases where laboratories need to distinguish between endogenous and exogenous sources, isotope ratio mass spectrometry can provide another layer of information.GC-IRMS can help laboratories determine whether selected urinary steroids are consistent with an endogenous or exogenous origin by comparing carbon isotope ratios.The result is an analytical toolkit in which different technologies answer different questions—from broad screening and sensitive confirmation to determining the origin of a compound.<h3>Putting advanced workflows into practice</h3>Technology is only part of the equation. Real-world anti-doping laboratories also need to manage high sample volumes, turnaround expectations and continually changing analytical requirements.At Laboratoire Anti-Dopage Français (LADF), Director Dr. Magnus Ericsson and his team use advanced mass spectrometry workflows to support anti-doping testing.According to Ericsson, integration of <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=20&amp;manufacturerItems=3">Thermo Scientific TSQ</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=37&amp;manufacturerItems=3&amp;search=vanquish">Vanquish systems</a> has helped enhance efficiency and turnaround while supporting more than 40,000 sample analyses since 2023.Experiences like these demonstrate how integrated analytical workflows can help laboratories translate advances in mass spectrometry into routine anti-doping operations.<h3>Preparing for what comes next</h3>Anti-doping science has advanced dramatically—from early testing approaches to today’s sophisticated, — mass spectrometry technologies; including but not limited to <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/products?category=20&amp;manufacturerItems=3">GC-MS/MS</a>, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?category=20&amp;manufacturerItems=3">LC-MS/MS</a>, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=22&amp;manufacturerItems=3">high-resolution accurate-mass MS</a> and<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/products?category=351&amp;manufacturerItems=3"> isotope ratio MS</a> workflows.But the science cannot stand still.Emerging compounds, evolving regulations and increasingly sophisticated doping strategies mean laboratories must continually evaluate how they manage and process samples and then screen, identify, quantify and confirm prohibited substances. The goal remains constant: provide accurate, high-quality analytical results that help protect the integrity of competition.Explore the technologies, workflows and expert perspectives shaping the next generation of anti-doping testing in our comprehensive eBook, Upholding the Integrity of Sport.<blockquote><h5><a target="_blank" rel="noopener noreferrer" href="https://documents.thermofisher.com/TFS-Assets/CMD/brochures/eb-004467-cl-cmd-ms-antidoping-eb004467-na-en.pdf">Download the anti-doping eBook</a></h5></blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#AntiDoping #MassSpectrometry #SportsIntegrity #WADA

Advancing Anti-Doping Science: Analytical Strategies for the Evolving Fight Against Doping

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Thymagen (Thymogen) analysis

Targeted HPLC analysis of Thymagen (Thymogen) in lyophilised vials and raw API powders, including identity, amount, and peptide purity.

The Thymagen (Thymogen) Analysis is a targeted laboratory test designed to confirm the identity, determine the amount, and assess the peptide purity of Thymagen in lyophilised vials and raw active pharmaceutical ingredient (API) powders.For lyophilised samples, results are reported in milligrams per vial together with peptide purity. Raw API powders can be evaluated using purity in % (m/m) together with peptide purity.<h4>About Thymagen (Thymogen)</h4>Thymagen, also known as Thymogen, is a synthetic dipeptide composed of glutamic acid and tryptophan (Glu-Trp). It has been studied primarily for its immunomodulatory properties and its effects on immune cell function.Research has focused particularly on its influence on T-cell differentiation and other processes involved in cellular and humoral immune responses.<h4>Key Features</h4><ul><li>Targeted identification and quantification of Thymagen</li><li>Determination of peptide purity</li><li>Suitable for lyophilised vials and raw API powders</li><li>Analysis by HPLC</li><li>Results for lyophilised samples reported in mg per vial and peptide purity</li><li>Alternative result format for raw API powders includes purity in % (m/m) and peptide purity</li><li>Minimum sample amount of one whole unopened vial with lyophilisate</li><li>Minimum sample amount of 30 mg for raw API powders</li><li>Average turnaround time of 7 business days</li></ul><h4>Analysis Parameters</h4><ul><li>Target compound: Thymagen</li><li>Other name: Thymogen</li><li>CAS number: 38101-59-6</li><li>Molecular weight: 333.34</li><li>Sequence: NH2-Glu-Trp-OH</li><li>Testing method: HPLC</li><li>Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial</li><li>Result format for lyophilised samples: mg per vial and peptide purity</li><li>Result format for raw API powders: Purity in % (m/m) and peptide purity</li><li>Minimum sample amount for lyophilised samples: One whole unopened vial with lyophilisate</li><li>Minimum sample amount for raw API powders: 30 mg in a sealed glass vial to prevent the sample from absorbing moisture</li><li>Average turnaround time: 7 business days</li></ul><blockquote>Important Notice<ul><li>This analysis is specifically intended for Thymagen (Thymogen). It does not automatically screen the sample for other peptides, related compounds, degradation products, contaminants, or undeclared substances.</li><li>For lyophilised samples, one whole unopened vial containing lyophilisate is required.</li><li>Raw API powder should be supplied as at least 30 mg in a sealed glass vial to protect the sample from moisture.</li><li>The alternative result format for raw API powders includes both purity in % (m/m) and peptide purity.</li></ul></blockquote><h4>Optional Additional Analyses</h4>The following optional laboratory services are available:<ul><li>TFA Analysis</li><li>Complete Raw Analytical Data</li><li>Fentanyl Screening</li><li>Alternative Result Format: Purity in % (m/m)</li><li>Batch Variance Testing</li><li>pH Measurement</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2522">Endotoxin Analysis</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2526">CHNS Mass Report</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2524">LCMS Screening for Peptide Contamination</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2527">Sterility Testing (TAMC + TYMC)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2525">Heavy Metals Analysis (As, Cd, Pb, Hg)</a></li><li>Additional Laboratory Report</li></ul>

Thymalin/Thymulin analysis

Targeted HPLC analysis of Thymalin/Thymulin in lyophilised vials and raw API powders, including identity, amount, and peptide purity.

The Thymalin/Thymulin Analysis is a targeted laboratory test designed to confirm the identity, determine the amount, and assess the peptide purity of Thymalin/Thymulin in lyophilised vials and raw active pharmaceutical ingredient (API) powders.For lyophilised samples, results are reported in milligrams per vial together with peptide purity. Raw API powders can be evaluated using purity in % (m/m) together with peptide purity.<h4>About Thymalin/Thymulin</h4>Thymalin and Thymulin are substances associated with thymic function and have been studied primarily in relation to immune regulation. Thymulin is a naturally occurring peptide involved particularly in T-cell development and function, while Thymalin is a polypeptide complex isolated from the thymus and investigated for its immunomodulatory effects.<h4>Key Features</h4><ul><li>Targeted identification and quantification of Thymalin/Thymulin</li><li>Determination of peptide purity</li><li>Suitable for lyophilised vials and raw API powders</li><li>Analysis by HPLC</li><li>Results for lyophilised samples reported in mg per vial and peptide purity</li><li>Alternative result format for raw API powders includes purity in % (m/m) and peptide purity</li><li>Minimum sample amount of one whole unopened vial with lyophilisate</li><li>Minimum sample amount of 30 mg for raw API powders</li><li>Average turnaround time of 7 business days</li></ul><h4>Analysis Parameters</h4><ul><li>Target compound: Thymalin/Thymulin</li><li>CAS number: 78922-62-0</li><li>Molecular weight: 859</li><li>Sequence: H-Pyr-Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn-OH</li><li>Testing method: HPLC</li><li>Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial</li><li>Result format for lyophilised samples: mg per vial and peptide purity</li><li>Result format for raw API powders: Purity in % (m/m) and peptide purity</li><li>Minimum sample amount for lyophilised samples: One whole unopened vial with lyophilisate</li><li>Minimum sample amount for raw API powders: 30 mg in a sealed glass vial to prevent the sample from absorbing moisture</li><li>Average turnaround time: 7 business days</li></ul><blockquote>Important Notice<ul><li>This analysis is specifically intended for Thymalin/Thymulin according to the parameters specified above. It does not automatically screen the sample for other thymic peptides, related compounds, degradation products, contaminants, or undeclared substances.</li><li>For lyophilised samples, one whole unopened vial containing lyophilisate is required.</li><li>Raw API powder should be supplied as at least 30 mg in a sealed glass vial to protect the sample from moisture.</li><li>The alternative result format for raw API powders includes both purity in % (m/m) and peptide purity.</li></ul></blockquote><h4>Optional Additional Analyses</h4>The following optional laboratory services are available:<ul><li>TFA Analysis</li><li>Complete Raw Analytical Data</li><li>Fentanyl Screening</li><li>Alternative Result Format: Purity in % (m/m)</li><li>Batch Variance Testing</li><li>pH Measurement</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2522">Endotoxin Analysis</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2526">CHNS Mass Report</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2524">LCMS Screening for Peptide Contamination</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2527">Sterility Testing (TAMC + TYMC)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2525">Heavy Metals Analysis (As, Cd, Pb, Hg)</a></li><li>Additional Laboratory Report</li></ul>

Thymosin Alpha-1 analysis

Targeted HPLC analysis of Thymosin Alpha-1 in lyophilised vials and raw API powders, including identity, amount, and peptide purity.

The Thymosin Alpha-1 Analysis is a targeted laboratory test designed to confirm the identity, determine the amount, and assess the peptide purity of Thymosin Alpha-1 in lyophilised vials and raw active pharmaceutical ingredient (API) powders.For lyophilised samples, results are reported in milligrams per vial together with peptide purity. Raw API powders can be evaluated using purity in % (m/m) together with peptide purity.<h4>About Thymosin Alpha-1</h4>Thymosin Alpha-1 (Tα1) is a 28-amino-acid peptide involved in the regulation of immune responses. It has been studied for its immunomodulatory effects, including its influence on T cells, dendritic cells, and innate and adaptive immunity.<h4>Key Features</h4><ul><li>Targeted identification and quantification of Thymosin Alpha-1</li><li>Determination of peptide purity</li><li>Suitable for lyophilised vials and raw API powders</li><li>Analysis by HPLC</li><li>Results for lyophilised samples reported in mg per vial and peptide purity</li><li>Alternative result format for raw API powders includes purity in % (m/m) and peptide purity</li><li>Minimum sample amount of one whole unopened vial with lyophilisate</li><li>Minimum sample amount of 30 mg for raw API powders</li><li>Average turnaround time of 7 business days</li></ul><h4>Analysis Parameters</h4><ul><li>Target compound: Thymosin Alpha-1</li><li>Other name: TA-1</li><li>CAS number: 69521-94-4</li><li>Molecular weight: 3108</li><li>Sequence: Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-OH</li><li>Testing method: HPLC</li><li>Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial</li><li>Result format for lyophilised samples: mg per vial and peptide purity</li><li>Result format for raw API powders: Purity in % (m/m) and peptide purity</li><li>Minimum sample amount for lyophilised samples: One whole unopened vial with lyophilisate</li><li>Minimum sample amount for raw API powders: 30 mg in a sealed glass vial to prevent the sample from absorbing moisture</li><li>Average turnaround time: 7 business days</li></ul><blockquote>Important Notice<ul><li>This analysis is specifically intended for Thymosin Alpha-1. It does not automatically screen the sample for other thymosin peptides, related compounds, degradation products, contaminants, or undeclared substances.</li><li>For lyophilised samples, one whole unopened vial containing lyophilisate is required.</li><li>Raw API powder should be supplied as at least 30 mg in a sealed glass vial to protect the sample from moisture.</li><li>The alternative result format for raw API powders includes both purity in % (m/m) and peptide purity.</li></ul></blockquote><h4>Optional Additional Analyses</h4>The following optional laboratory services are available:<ul><li>TFA Analysis</li><li>Complete Raw Analytical Data</li><li>Fentanyl Screening</li><li>Alternative Result Format: Purity in % (m/m)</li><li>Batch Variance Testing</li><li>pH Measurement</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2522">Endotoxin Analysis</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2526">CHNS Mass Report</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2524">LCMS Screening for Peptide Contamination</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2527">Sterility Testing (TAMC + TYMC)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2525">Heavy Metals Analysis (As, Cd, Pb, Hg)</a></li><li>Additional Laboratory Report</li></ul>

Thymosin Alpha-1/ Thymalin (Thymulin) blend analysis

Targeted analysis of a Thymosin Alpha-1 and Thymalin/Thymulin blend for identification and amount determination of individual components.

The Thymosin Alpha-1 / Thymalin (Thymulin) Blend Analysis is a targeted laboratory test designed to confirm the identity and determine the amount of Thymosin Alpha-1 and Thymalin/Thymulin in a lyophilised peptide blend.Results are reported in milligrams per vial. Peptide purity cannot be determined in peptide blends because impurities from the individual components may overlap during analysis.<h4>About the Analyzed Substances</h4>Thymosin Alpha-1 (Tα1) is a 28-amino-acid peptide involved in immune regulation and studied primarily for its immunomodulatory effects.Thymalin and Thymulin are substances associated with thymic function and have also been studied in relation to immune regulation. Thymulin is a naturally occurring thymic peptide involved particularly in T-cell development and function, while Thymalin is a polypeptide complex isolated from the thymus and investigated for its immunomodulatory effects.<h4>Key Features</h4><ul><li>Targeted identification and quantification of Thymosin Alpha-1 and Thymalin/Thymulin</li><li>Designed for lyophilised peptide blends</li><li>Results reported in mg per vial</li><li>Minimum sample amount of one whole unopened vial with lyophilisate</li><li>Average turnaround time of 7 business days</li><li>Peptide purity is not determined for blends</li></ul><h4>Analysis Parameters</h4><ul><li>Target compounds: Thymalin/Thymulin and Thymosin Alpha-1</li><li>CAS number – TA-1: 69521-94-4</li><li>Molecular weight – TA-1: 3108</li><li>Sequence – TA-1: Ac-Ser-Asp-Ala-Ala-Val-Asp-Thr-Ser-Ser-Glu-Ile-Thr-Thr-Lys-Asp-Leu-Lys-Glu-Lys-Lys-Glu-Val-Val-Glu-Glu-Ala-Glu-Asn-OH</li><li>CAS number – Thymalin: 78922-62-0</li><li>Molecular weight – Thymalin: 859</li><li>Sequence – Thymalin: H-Pyr-Ala-Lys-Ser-Gln-Gly-Gly-Ser-Asn-OH</li><li>Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial</li><li>Result format: mg per vial</li><li>Minimum sample amount: One whole unopened vial with lyophilisate</li><li>Average turnaround time: 7 business days</li><li>Important note: Peptide purity is not measured</li></ul><blockquote>Important Notice<ul><li>Peptide purity cannot be determined in this blend because impurities from the individual components may overlap and cannot be reliably distinguished from one another.</li><li>This analysis is specifically intended for blends containing Thymosin Alpha-1 and Thymalin/Thymulin. It does not automatically screen the sample for other peptides, related compounds, contaminants, or undeclared substances.</li><li>For analysis, one whole unopened vial containing lyophilisate is required.</li></ul></blockquote><h4>Optional Additional Analyses</h4>The following optional laboratory services are available:<ul><li>TFA Analysis</li><li>Complete Raw Analytical Data</li><li>Fentanyl Screening</li><li>Batch Variance Testing</li><li>pH Measurement</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2522">Endotoxin Analysis</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2524">LCMS Screening for Peptide Contamination</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2527">Sterility Testing (TAMC + TYMC)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2525">Heavy Metals Analysis (As, Cd, Pb, Hg)</a></li><li>Additional Laboratory Report</li></ul>

Vesugen analysis

Targeted HPLC analysis of Vesugen in lyophilised vials and raw API powders, including identity, amount, and peptide purity determination.

The Vesugen Analysis is a targeted laboratory test designed to confirm the identity, determine the amount, and assess the peptide purity of Vesugen in lyophilised vials and raw active pharmaceutical ingredient (API) powders.For lyophilised samples, results are reported in milligrams per vial together with peptide purity. Raw API powders can be evaluated using purity in % (m/m) together with peptide purity.<h4>About Vesugen</h4>Vesugen is a synthetic tripeptide with the amino acid sequence Lys-Glu-Asp (KED). It has been studied primarily in experimental research involving vascular endothelium and cellular processes associated with vascular ageing.<h4>Key Features</h4><ul><li>Targeted identification and quantification of Vesugen</li><li>Determination of peptide purity</li><li>Suitable for lyophilised vials and raw API powders</li><li>Analysis by HPLC</li><li>Results for lyophilised samples reported in mg per vial and peptide purity</li><li>Alternative result format for raw API powders includes purity in % (m/m) and peptide purity</li><li>Minimum sample amount of one whole unopened vial with lyophilisate</li><li>Minimum sample amount of 30 mg for raw API powders</li><li>Average turnaround time of 7 business days</li></ul><h4>Analysis Parameters</h4><ul><li>Target compound: Vesugen</li><li>Molecular weight: 390</li><li>Sequence: Lys-Glu-Asp-OH</li><li>Testing method: HPLC</li><li>Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial</li><li>Result format for lyophilised samples: mg per vial and peptide purity</li><li>Result format for raw API powders: Purity in % (m/m) and peptide purity</li><li>Minimum sample amount for lyophilised samples: One whole unopened vial with lyophilisate</li><li>Minimum sample amount for raw API powders: 30 mg in a sealed glass vial to prevent the sample from absorbing moisture</li><li>Average turnaround time: 7 business days</li></ul><blockquote>Important Notice<ul><li>This analysis is specifically intended for Vesugen. It does not automatically screen the sample for other peptides, related compounds, degradation products, contaminants, or undeclared substances.</li><li>For lyophilised samples, one whole unopened vial containing lyophilisate is required.</li><li>Raw API powder should be supplied as at least 30 mg in a sealed glass vial to protect the sample from moisture.</li><li>The alternative result format for raw API powders includes both purity in % (m/m) and peptide purity.</li></ul></blockquote><h4>Optional Additional Analyses</h4>The following optional laboratory services are available:<ul><li>TFA Analysis</li><li>Complete Raw Analytical Data</li><li>Fentanyl Screening</li><li>Alternative Result Format: Purity in % (m/m)</li><li>Batch Variance Testing</li><li>pH Measurement</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2522">Endotoxin Analysis</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2526">CHNS Mass Report</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2524">LCMS Screening for Peptide Contamination</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2527">Sterility Testing (TAMC + TYMC)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2525">Heavy Metals Analysis (As, Cd, Pb, Hg)</a></li><li>Additional Laboratory Report</li></ul>

Vilon analysis

Targeted HPLC analysis of Vilon in lyophilised vials and raw API powders, including identity, amount, and peptide purity determination.

The Vilon Analysis is a targeted laboratory test designed to confirm the identity, determine the amount, and assess the peptide purity of Vilon in lyophilised vials and raw active pharmaceutical ingredient (API) powders.For lyophilised samples, results are reported in milligrams per vial together with peptide purity. Raw API powders can be evaluated using purity in % (m/m) together with peptide purity.<h4>About Vilon</h4>Vilon is a synthetic dipeptide composed of lysine and glutamic acid (Lys-Glu). It has been studied primarily for its immunomodulatory effects, including its influence on T-cell differentiation and thymic immune function.<h4>Key Features</h4><ul><li>Targeted identification and quantification of Vilon</li><li>Determination of peptide purity</li><li>Suitable for lyophilised vials and raw API powders</li><li>Analysis by HPLC</li><li>Results for lyophilised samples reported in mg per vial and peptide purity</li><li>Alternative result format for raw API powders includes purity in % (m/m) and peptide purity</li><li>Minimum sample amount of one whole unopened vial with lyophilisate</li><li>Minimum sample amount of 30 mg for raw API powders</li><li>Average turnaround time of 7 business days</li></ul><h4>Analysis Parameters</h4><ul><li>Target compound: Vilon</li><li>CAS number: 45234-02-04</li><li>Molecular weight: 275.3</li><li>Sequence: NH2-Lys-Glu-OH</li><li>Testing method: HPLC</li><li>Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial</li><li>Result format for lyophilised samples: mg per vial and peptide purity</li><li>Result format for raw API powders: Purity in % (m/m) and peptide purity</li><li>Minimum sample amount for lyophilised samples: One whole unopened vial with lyophilisate</li><li>Minimum sample amount for raw API powders: 30 mg in a sealed glass vial to prevent the sample from absorbing moisture</li><li>Average turnaround time: 7 business days</li></ul><blockquote>Important Notice<ul><li>This analysis is specifically intended for Vilon. It does not automatically screen the sample for other peptides, related compounds, degradation products, contaminants, or undeclared substances.</li><li>For lyophilised samples, one whole unopened vial containing lyophilisate is required.</li><li>Raw API powder should be supplied as at least 30 mg in a sealed glass vial to protect the sample from moisture.</li><li>The alternative result format for raw API powders includes both purity in % (m/m) and peptide purity.</li></ul></blockquote><h4>Optional Additional Analyses</h4>The following optional laboratory services are available:<ul><li>TFA Analysis</li><li>Complete Raw Analytical Data</li><li>Fentanyl Screening</li><li>Alternative Result Format: Purity in % (m/m)</li><li>Batch Variance Testing</li><li>pH Measurement</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2522">Endotoxin Analysis</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2526">CHNS Mass Report</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2524">LCMS Screening for Peptide Contamination</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2527">Sterility Testing (TAMC + TYMC)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2525">Heavy Metals Analysis (As, Cd, Pb, Hg)</a></li><li>Additional Laboratory Report</li></ul>

VIP analysis

Targeted HPLC analysis of VIP in lyophilised vials and raw API powders, including identity, amount, and peptide purity determination.

The VIP Analysis is a targeted laboratory test designed to confirm the identity, determine the amount, and assess the peptide purity of VIP in lyophilised vials and raw active pharmaceutical ingredient (API) powders.For lyophilised samples, results are reported in milligrams per vial together with peptide purity. Raw API powders can be evaluated using purity in % (m/m) together with peptide purity.<h4>About VIP</h4>VIP (vasoactive intestinal peptide), also known as aviptadil, is a naturally occurring 28-amino-acid neuropeptide. It is involved in vasodilation, smooth muscle relaxation, digestive functions, and regulation of the nervous and immune systems.<h4>Key Features</h4><ul><li>Targeted identification and quantification of VIP</li><li>Determination of peptide purity</li><li>Suitable for lyophilised vials and raw API powders</li><li>Analysis by HPLC</li><li>Results for lyophilised samples reported in mg per vial and peptide purity</li><li>Alternative result format for raw API powders includes purity in % (m/m) and peptide purity</li><li>Minimum sample amount of one whole unopened vial with lyophilisate</li><li>Minimum sample amount of 30 mg for raw API powders</li><li>Average turnaround time of 7 business days</li></ul><h4>Analysis Parameters</h4><ul><li>Target compound: VIP</li><li>Other name: Aviptadil</li><li>CAS number: 40077-57-4</li><li>Molecular weight: 3327</li><li>Sequence: H-His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-OH</li><li>Testing method: HPLC</li><li>Minimum amount of target compound for evaluation: Less than 1/20 of the usual dosage per vial</li><li>Result format for lyophilised samples: mg per vial and peptide purity</li><li>Result format for raw API powders: Purity in % (m/m) and peptide purity</li><li>Minimum sample amount for lyophilised samples: One whole unopened vial with lyophilisate</li><li>Minimum sample amount for raw API powders: 30 mg in a sealed glass vial to prevent the sample from absorbing moisture</li><li>Average turnaround time: 7 business days</li></ul><blockquote>Important Notice<ul><li>This analysis is specifically intended for VIP. It does not automatically screen the sample for related peptides, degradation products, contaminants, or undeclared substances.</li><li>For lyophilised samples, one whole unopened vial containing lyophilisate is required.</li><li>Raw API powder should be supplied as at least 30 mg in a sealed glass vial to protect the sample from moisture.</li><li>The alternative result format for raw API powders includes both purity in % (m/m) and peptide purity.</li></ul></blockquote><h4>Optional Additional Analyses</h4>The following optional laboratory services are available:<ul><li>TFA Analysis</li><li>Complete Raw Analytical Data</li><li>Fentanyl Screening</li><li>Alternative Result Format: Purity in % (m/m)</li><li>Batch Variance Testing</li><li>pH Measurement</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2522">Endotoxin Analysis</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2526">CHNS Mass Report</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2524">LCMS Screening for Peptide Contamination</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2527">Sterility Testing (TAMC + TYMC)</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2525">Heavy Metals Analysis (As, Cd, Pb, Hg)</a></li><li>Additional Laboratory Report</li></ul>

Thermo Scientific SpeedVac SRF110 Refrigerated Centrifugal Vacuum Concentrator 

Thermo Scientific™ SpeedVac™ SRF110 is a refrigerated centrifugal vacuum concentrator for gentle concentration of heat-sensitive biological samples, proteins, enzymes, buffers, and HPLC fractions.

The Thermo Scientific™ SpeedVac™ SRF110 Refrigerated Centrifugal Vacuum Concentrator is designed for the gentle concentration and evaporation of heat-sensitive biological samples, including proteins, enzymes, peptides, and other biomolecules. Combining centrifugal force, vacuum, and precise temperature control, the system efficiently removes solvents while helping preserve sample integrity and minimize the risk of thermal degradation.The SRF110 is particularly suitable for the evaporation of non-aggressive, aqueous-based solvents, buffers, and HPLC fractions. Programmable temperature control allows the chamber temperature to be maintained before, during, and after a run, providing greater flexibility for temperature-sensitive applications. The operating temperature can be set from –4 °C to 100 °C in 1 °C increments.Its modular design allows the system to be configured according to individual laboratory requirements. Users can select from compatible Thermo Scientific vacuum pumps, vapor traps, tubing, rotors, and other accessories to build a customized vacuum concentration setup. The SRF110 features a self-aligning, quick-release rotor mechanism, and the RF132 hexagonal rotor is supplied with the concentrator. Additional rotor options are available for different sample formats and capacities.<h4>Key features</h4><ul><li>Refrigerated centrifugal vacuum concentrator for heat-sensitive biological samples</li><li>Suitable for aqueous, non-aggressive solvents, buffers, and HPLC fractions</li><li>Temperature range from –4 °C to 100 °C, adjustable in 1 °C increments</li><li>Programmable chamber temperature control before and after sample runs</li><li>3 preset programs with storage for up to 9 programs</li><li>Self-aligning, quick-release rotor mechanism</li><li>RF132 hexagonal rotor included</li><li>Additional rotor options available for different applications</li><li>Modular configuration for flexible integration of pumps, vapor traps, and accessories</li><li>Electrical options: 115 V AC, 60 Hz, 16 A or 230 V AC, 50 Hz, 8 A</li><li>Certifications include UL, CE, UKCA, KC, and RCM</li><li>F-gas compliant for applicable EPA-SNAP and EU requirements</li></ul><h5>Two kit configurations are available:</h5><ol><li>The SRF110P1 modular configuration includes the refrigerated SRF110 concentrator, RF132 rotor, VLP120-115 oil pump, RVT450-115 vapor trap, universal tubing, 4 L glass condensation flask with cap, and CryoCool™ heat-transfer fluid.&nbsp;</li><li>The SRF110P2 semi-integrated configuration combines the SRF110 and RF132 rotor with the UVS450 Universal Vacuum System, universal tubing, condensation flask, cap, and CryoCool™ fluid.</li></ol>

Thermo Scientific Orbitrap Tribrid Apex Mass Spectrometer

Thermo Scientific Orbitrap Tribrid Apex combines a quadrupole, linear ion trap, and Orbitrap analyzer for high-resolution MSⁿ workflows in proteomics, biopharma, structural biology, and small-molecule analysis.

The Thermo Scientific Orbitrap Tribrid Apex is a new-generation high-resolution hybrid mass spectrometry platform designed for complex structural characterization, multiomics, biopharmaceutical research, structural biology, and small-molecule analysis. It combines a quadrupole mass filter, high-capacity linear ion trap, and Orbitrap mass analyzer with advanced ion parallelization, flexible MSⁿ workflows, and a broad range of fragmentation technologies. The platform is engineered to deliver higher speed, sensitivity, fragmentation flexibility, and confident characterization across demanding biological and chemical applications.&nbsp;Orbitrap Tribrid Apex is available in four application-focused editions: Small Molecule, MultiOmics, Structural Biology, and BioPharma. Each is built on the common Tribrid architecture while providing capabilities tailored to specific analytical workflows.&nbsp;<h4>Key Features</h4><ul><li>Hybrid Tribrid architecture:<ul><li>QR5 segmented quadrupole mass filter</li><li>high-capacity linear ion trap</li><li>Orbitrap mass analyzer</li></ul></li><li>Orbitrap resolution &gt;480,000 FWHM</li><li>Orbitrap acquisition rates up to 75 Hz with TurboTMT</li><li>MSⁿ acquisition in the linear ion trap at up to 100 Hz</li><li>Dual Ion Routing Multipoles for ion parallelization</li><li>Precursor isolation widths down to 0.4 m/z</li><li>Extensive fragmentation capabilities including:<ul><li>HCD</li><li>CID</li><li>ETD</li><li>EThcD</li><li>ETciD</li><li>optional UVPD</li><li>optional IRMPD</li></ul></li><li>Flexible multistage MSⁿ workflows</li><li>Real-Time Library Search and intelligent acquisition strategies</li><li>Auto-Ready 2 ion source for automated and remote calibration</li><li>Oil-free dry pump supplied as standard</li><li>Optional EASY-IC internal calibration for sub-1 ppm mass accuracy</li><li>Optional Native MS, PTCR, and Direct Mass Technology workflows depending on edition and configuration.&nbsp;</li></ul><h3>Application-Focused Editions</h3><h4>Orbitrap Tribrid Apex Small Molecule MS</h4>Designed for unknown identification, structural elucidation, metabolomics, lipidomics, and combined discovery/quantitation workflows. Multiple orthogonal fragmentation pathways can be combined within a single experiment to provide complementary structural information.SQUAD—Simultaneous Quantitation and Discovery—combines targeted quantitation and untargeted compound discovery in a single LC-MS analysis. The brochure demonstrates a seven-order linear dynamic range and reports 40% faster polarity switching in the illustrated workflow.<h4>Orbitrap Tribrid Apex MultiOmics MS</h4>Designed for proteomics and broader multiomics workflows, supporting DDA, DIA, hybrid DIA, TMT multiplexing, PRM, glycopeptide analysis, and PTM characterization. Improved ion parallelization and IR-assisted fragmentation enhance peptide characterization and quantitative productivity.&nbsp;<h4>Orbitrap Tribrid Apex Structural Biology MS</h4>Combines Native MS, top-down analysis, flexible MSⁿ fragmentation, and Direct Mass Technology mode to investigate intact protein complexes, molecular interactions, and structural dynamics.With the Native MS option:<ul><li>MS1 range extends to m/z 16,000</li><li>MS²/MSⁿ range extends to m/z 20,000</li><li>quadrupole isolation extends to m/z 12,000</li><li>ion-trap isolation extends to m/z 8,000.</li></ul><h4>Orbitrap Tribrid Apex BioPharma MS</h4>Designed for comprehensive biotherapeutic characterization from intact mass and top-/middle-down analysis through peptide mapping. Representative workflows include monoclonal antibodies, ADCs, proteoforms, oligonucleotides, and tRNA.IR-assisted EThcD combined with PTCR MS³ can reduce spectral congestion and increase sequence coverage for heterogeneous biotherapeutics, while Direct Mass Technology mode supports interpretation of complex top- and middle-down spectra.&nbsp;<h3>Tribrid Architecture and Ion Parallelization</h3>The platform incorporates two Ion Routing Multipoles:<ul><li>the front IRM performs HCD at the MS² stage,</li><li>the rear IRM supports parallel ion processing and HCD at MS³ and higher stages.</li></ul>The QR5 segmented quadrupole with hyperbolic surfaces supports precursor isolation widths down to 0.4 m/z, while parallelized ion handling improves throughput in complex MSⁿ workflows.&nbsp;<h4>Fragmentation Capabilities</h4>Available fragmentation approaches include:<ul><li>CID – collision-induced dissociation</li><li>HCD – higher-energy collisional dissociation</li><li>ETD – electron transfer dissociation</li><li>EThcD – ETD with supplemental HCD</li><li>ETciD – ETD with supplemental CID</li><li>UVPD – ultraviolet photodissociation, optional</li><li>IRMPD – infrared multiphoton dissociation, optional</li></ul>The UV-laser option supports applications such as top-down proteomics, oligonucleotides, and lipidomics, while the IR-laser option supports TMT reporter-ion fragmentation, top-down MS, and peptide workflows.&nbsp;<h4>Direct Mass Technology Mode</h4>Optional Direct Mass Technology mode uses single-ion detection to assign charge without relying on conventional isotope or charge-state patterns. It expands characterization capabilities for intact proteins, protein complexes, proteoforms, heterogeneous biologics, and complex top-down spectra.&nbsp;<h4>Intelligent Acquisition</h4>The platform supports intelligent acquisition tools including:<ul><li>Real-Time Library Search</li><li>Adaptive Real-Time Library Search</li><li>Smart Targeted TMT</li><li>spectral-library-directed MSⁿ</li><li>pre-built method templates.&nbsp;</li></ul><h4>Available Editions and Options</h4>Instrument Editions<ul><li>B51008787 – Orbitrap Tribrid Apex Structural Biology MS</li><li>B51008788 – Orbitrap Tribrid Apex BioPharma MS</li><li>B51008789 – Orbitrap Tribrid Apex MultiOmics MS</li><li>B51008790 – Orbitrap Tribrid Apex Small Molecule MS</li></ul>Listed Options<ul><li>B51008633 – UV-laser option for Tribrid MS</li><li>B51008634 – IR-laser option for Tribrid MS</li><li>B51009083 – Native MS option for Tribrid MS</li><li>B51009084 – Direct Mass Technology mode option for Tribrid MS</li></ul><h4>Typical Applications</h4><ul><li>Proteomics and multiomics</li><li>TMT and label-free quantitation</li><li>Glycoproteomics and PTM analysis</li><li>Top-down and middle-down proteomics</li><li>Native MS</li><li>HDX-MS</li><li>Structural biology</li><li>Monoclonal antibodies</li><li>ADCs and other biotherapeutics</li><li>Oligonucleotides and RNA</li><li>Lipidomics</li><li>Metabolomics</li><li>Small-molecule analysis</li><li>Unknown identification</li><li>Structural elucidation</li><li>Targeted and untargeted quantitation</li></ul>

ProtiFi S-Trap Micro Spin Columns for Proteomics Sample Preparation

ProtiFi S-Trap Micro Spin Columns process 1–100 µg of total protein using SDS-compatible cleanup and in-column digestion. The workflow efficiently removes MS-incompatible contaminants and yields peptides ready for LC-MS/MS analysis.

ProtiFi S-Trap™ Micro Spin Columns are centrifugal sample-preparation columns designed for rapid and reproducible protein processing prior to LC-MS proteomics. The patented S-Trap™ technology enables robust SDS-based sample lysis and solubilization, followed by trapping of denatured, undigested proteins within the porous S-Trap™ matrix. MS-incompatible contaminants are removed during washing, and captured proteins can then be enzymatically digested directly within the column.&nbsp;The Micro format is intended for samples containing 1–100 µg of total protein. The workflow combines solubilization, reduction, alkylation, protein trapping, cleanup, enzymatic digestion, and peptide elution for subsequent LC-MS analysis.<h3>S-Trap™ Technology</h3>In a standard S-Trap™ workflow, proteins are first solubilized in an SDS-containing environment. Subsequent acidification to pH ≤1 and exposure to a high concentration of methanol fully denature the proteins. The denatured proteins are then captured within the pores of the derivatized S-Trap™ matrix through multiple weak-affinity interactions.Washing removes contaminants that can interfere with downstream LC-MS analysis, including:<ul><li>SDS and other detergents</li><li>salts</li><li>buffers</li><li>PEG</li><li>glycerol</li><li>stabilizers</li><li>excipients</li><li>Laemmli loading buffer components</li></ul>After protease addition, the trapped proteins are digested within the porous matrix. According to ProtiFi, the S-Trap™ matrix does not retain the resulting peptides, allowing them to be eluted after digestion for downstream analysis.<h4>Standard Workflow</h4><ol><li>Protein solubilization and denaturation in SDS</li><li>Reduction and alkylation</li><li>Sample acidification</li><li>Addition of methanolic Binding/Wash Solution</li><li>Protein trapping in the S-Trap™ Micro Spin Column</li><li>Washing to remove contaminants</li><li>Protease addition</li><li>In-column digestion</li><li>Peptide elution</li><li>Preparation for downstream LC-MS analysis</li></ol><h4>Technical Specifications</h4><ul><li>Format: Centrifugal proteomics sample-preparation column</li><li>Recommended Total Protein Amount: 1–100 µg</li><li>Maximum Stated Capacity: ≤100 µg total protein</li><li>Approximate Column Reservoir Capacity: 275 µL</li><li>Standard Centrifugation: 10,000 × g</li><li>Maximum Centrifugation when required: up to 15,000 × g</li><li>Recommended Digestion in Current Protocol: 2 h at 47 °C</li><li>Protease Compatibility: application dependent; e.g., trypsin or Trypsin/Lys-C</li><li>Flow Methods:<ul><li>centrifugation</li><li>optional vacuum</li><li>optional positive pressure</li></ul></li><li>Downstream Technique: LC-MS / LC-MS/MS</li><li>Intended Use: Research Use Only – not for diagnostic procedures&nbsp;</li></ul><h4>Available Pack Sizes</h4><ul><li>C002-MICRO-0010PK – 10 S-Trap™ Micro Spin Columns</li><li>C002-MICRO-0040PK – 40 S-Trap™ Micro Spin Columns</li><li>C002-MICRO-0080PK – 80 S-Trap™ Micro Spin Columns&nbsp;</li></ul><blockquote><h5>Package Contents</h5>This product contains S-Trap™ Micro Spin Columns only. Buffers, reduction and alkylation reagents, protease, and other workflow reagents are not included. ProtiFi separately offers the S-Trap™ Micro Kit, which combines Micro Spin Columns with premade S-Trap™ solutions.</blockquote>

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