ProtiFi S-Trap™ Micro Spin Columns are centrifugal sample-preparation columns designed for rapid and reproducible protein processing prior to LC-MS proteomics. The patented S-Trap™ technology enables robust SDS-based sample lysis and solubilization, followed by trapping of denatured, undigested proteins within the porous S-Trap™ matrix. MS-incompatible contaminants are removed during washing, and captured proteins can then be enzymatically digested directly within the column.
The Micro format is intended for samples containing 1–100 µg of total protein. The workflow combines solubilization, reduction, alkylation, protein trapping, cleanup, enzymatic digestion, and peptide elution for subsequent LC-MS analysis.
S-Trap™ Technology
In a standard S-Trap™ workflow, proteins are first solubilized in an SDS-containing environment. Subsequent acidification to pH ≤1 and exposure to a high concentration of methanol fully denature the proteins. The denatured proteins are then captured within the pores of the derivatized S-Trap™ matrix through multiple weak-affinity interactions.
Washing removes contaminants that can interfere with downstream LC-MS analysis, including:
- SDS and other detergents
- salts
- buffers
- PEG
- glycerol
- stabilizers
- excipients
- Laemmli loading buffer components
After protease addition, the trapped proteins are digested within the porous matrix. According to ProtiFi, the S-Trap™ matrix does not retain the resulting peptides, allowing them to be eluted after digestion for downstream analysis.
Standard Workflow
- Protein solubilization and denaturation in SDS
- Reduction and alkylation
- Sample acidification
- Addition of methanolic Binding/Wash Solution
- Protein trapping in the S-Trap™ Micro Spin Column
- Washing to remove contaminants
- Protease addition
- In-column digestion
- Peptide elution
- Preparation for downstream LC-MS analysis
Technical Specifications
- Format: Centrifugal proteomics sample-preparation column
- Recommended Total Protein Amount: 1–100 µg
- Maximum Stated Capacity: ≤100 µg total protein
- Approximate Column Reservoir Capacity: 275 µL
- Standard Centrifugation: 10,000 × g
- Maximum Centrifugation when required: up to 15,000 × g
- Recommended Digestion in Current Protocol: 2 h at 47 °C
- Protease Compatibility: application dependent; e.g., trypsin or Trypsin/Lys-C
- Flow Methods:
- centrifugation
- optional vacuum
- optional positive pressure
- Downstream Technique: LC-MS / LC-MS/MS
- Intended Use: Research Use Only – not for diagnostic procedures
Available Pack Sizes
- C002-MICRO-0010PK – 10 S-Trap™ Micro Spin Columns
- C002-MICRO-0040PK – 40 S-Trap™ Micro Spin Columns
- C002-MICRO-0080PK – 80 S-Trap™ Micro Spin Columns
Package Contents
This product contains S-Trap™ Micro Spin Columns only. Buffers, reduction and alkylation reagents, protease, and other workflow reagents are not included. ProtiFi separately offers the S-Trap™ Micro Kit, which combines Micro Spin Columns with premade S-Trap™ solutions.
