What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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<h3>Solving Problems That Matter - a Waters webinar series featuring USP speakers</h3>
<h3>Episode 4: aQbD for OTC products</h3>
Summary
Analyzing over-the-counter formulations can be overwhelming due to the wide range of APIs and excipients used in varying concentrations. These excipients can cause problematic matrix effects and coelutions, making the analysis of formulations complex. Our panelists will discuss how to leverage aQbD software to develop a single chromatographic method suitable to analyze multiple APIs in a variety of formulations.
Key learning objective:
Learn how to leverage aQbD tools to develop a single method suitable for a variety of OTC formulations.
Who sould attend:
Pharmaceutical method developers 
<blockquote>
Presenter: Fadi Alkhateeb (Principle Scientist, Waters Corporations)
</blockquote>
<blockquote>
Dr. Fadi Alkhateeb is a Senior Scientist in the Scientific Operations Department at WatersCorporation HQ in Milford, MA USA. He joined Waters in 2017 and since then he has beenmainly focusing on exploring various approaches for analytical method development forpharmaceutical drugs. Prior to joining Waters, he was a postdoctoral fellow at the University ofCalgary (Calgary, AB, Canada), where he did research at Alberta Sulphur Research, one ofNorth America&#39;s leading Sulphur research institutes. He received his PhD from the University ofCalgary, focusing on the development of novel, rapid separation and sample preparationtechniques for the analysis of a wide range of chemical compounds. Dr. Alkhateeb publishedseveral peer-reviewed articles in top analytical journals.​
</blockquote>
<blockquote>
Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)
</blockquote>
<blockquote>
Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.
</blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

aQbD for OTC products

Solving Problems That Matter - a Waters webinar series featuring USP speakers. Episode 4: aQbD for OTC products.

<h3>LC-MS/MS 201 webinar series</h3>
Advanced techniques for data acquisition and processing
<h4>Elevate your analytical expertise with advanced techniques and workflows</h4>
Sharpen your data acquisition and processing skills with our LC-MS/MS 201 webinar series. Based on our LC-MS/MS 101 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows. Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.
<h3>This webinar:</h3>
<h4>May 16, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - Excel strategies for mass spectrometry data analysis</h5>
<ul>
<li>Take an in-depth look at when and how to use Excel to increase your productivity.</li>
</ul>
<h3>Other webinars from the series:</h3>
<h4>March 21, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3705">Mastering chromatography</a></h5>
<ul>
<li>Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.</li>
</ul>
<h4>April 4, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - <a href="(https://lcms.labrulez.com/webinar/3706)">It&#39;s a TRAP! Getting the most out of your QTRAP system</a></h5>
<ul>
<li>Learn when to utilize QTRAP functionality to create more selective and sensitive methods.</li>
</ul>
<h4>April 18, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3707">Troubleshooting at the source</a></h5>
<ul>
<li>Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.</li>
</ul>
<h4>May 2, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3708">Automating data processing</a></h5>
<ul>
<li>Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.</li>
</ul>

Excel strategies for mass spectrometry data analysis

Take an in-depth look at when and how to use Excel to increase your productivity.

<h4>EU Live Interactive Web Series</h4>
Gather on Thursday or Friday at 11 AM or 12 PM CET for our live interactive web series.
Join us for an engaging journey into the world of advanced molecular analysis with our EU Live Interactive Web Series, hosted by Waters | Wyatt Technology! Dive deep into the intricacies of SEC-MALS and FFF-MALS systems, enhance your skills in ASTRA and DYNAMICS data processing, and master the measurement of dn/dc values—crucial for your research.
We dedicate 60 minutes to explore a specific topic, accompanied by a live discussion where all your questions are welcome. Whether you&#39;re dealing with polydisperse samples, eager to optimize your error analysis techniques, or curious about batch MALS work, our sessions are designed to elevate your expertise.
What&#39;s more, you’ll learn how to achieve stable RI baselines and utilize ASTRA&#39;s robust distribution analysis and results fitting features. Our web series is an invaluable, free resource exclusively for Wyatt customers, promising a 100% live, interactive learning experience.
Don’t miss out on this opportunity to connect, learn, and grow.
The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all Wyatt customers.
The EU live interactive web series are held on select Thursday or Friday afternoons at 11 AM or 12 PM CET.
May 3rd at 11:00 AM : MALS
<ul>
<li>How MALS can characterize molar mass, radius and A2?</li>
<li>How does a DAWN detector work?</li>
</ul>
May 17th at 11:00 AM : SEC-MALS
<ul>
<li>Applications and data interpretation</li>
<li>Data analysis in ASTRA</li>
</ul>
June 7th at 11:00 AM : RI &amp; dn/dc
<ul>
<li>A perfect match: MALS and RI</li>
<li>The importance of dn/dc and how to determine it</li>
</ul>
June 21st at 11:00 AM : DLS 
<ul>
<li>Benefits of measuring the Hydrodynamic Radius (Rh)</li>
<li>Dynamic Light Scattering in cuvette, high throughput and online</li>
</ul>
July 5th at 11:00 AM : Dynamics data processing tips &amp; tricks
<ul>
<li>Data interpretation and data filter</li>
<li>Use your Dyna Pro instrument to get: colloidal stability (kD/A2), thermal stability, viscosity or particle concentration</li>
</ul>
<blockquote>
Presenter: Dr. Christian Ackerschott
</blockquote>
<blockquote>
Dr. Christian Ackerschott studied Chemistry at the University of Cologne and finished with his diploma thesis in Biochemistry. He completed his studies with the PhD thesis “Characterization of Recombinant Flagelliform Spider Silk Proteins” at the Technische Universität München, where AF4-MALS was used as an essential characterization method.
</blockquote>
<blockquote>
Since 2007, Christian works at Wyatt Technology as Application Scientist and is now Head of Customer Service. His tasks also include customer trainings in Dernbach and onsite as well as education and training of the local Field Application Scientists.
</blockquote>

Waters Corporation: EU Live Interactive Web Series

SEC-MALS

SEC-MALS - Applications and data interpretation. Data analysis in ASTRA.

Listeria monocytogenes, a foodborne pathogen, poses a particular risk to vulnerable populations such as infants, the elderly, immunocompromised individuals, and pregnant women. Timely and accurate identification helps to prevent outbreaks of listeriosis and is essential for ensuring food safety and protection of public health.
In this webinar, Dr. Helene Oberreuter, food microbiologist and senior government councilor, will highlight a validated workflow for the differentiation of Listeria monocytogenes serogroups by FT-IR spectroscopy. The workflow consists of species identification by MALDI-TOF mass spectrometry (MALDI Biotyper®) followed by serogroup differentiation with the IR Biotyper® using a classifier which is based on an artificial neural network.
Key learning objectives
<ul>
<li>Explore the use of FT-IR spectroscopy in food pathogen detection
</li>
<li>Discover a validated workflow for serogroup differentiation
</li>
<li>Learn how to apply an artificial neural network to pathogen classification
</li>
<li>Gain insights into the preselection of samples by FT-IR for whole genome sequencing
</li>
</ul>
<blockquote>
Presenter: Dr. Helene Oberreuter (Food Microbiologist and Senior Government Councilor, CVUA Stuttgart, Germany)
</blockquote>
<blockquote>
Presenter: Lawrence Howes (SelectScience)
</blockquote>
Who should attend?
Those working in food microbiology laboratories, reference laboratories and centers for food pathogens, state and public health laboratories, and centers for disease control and prevention
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Bruker - Validated differentiation of Listeria monocytogenes by FT-IR spectroscopy using an artificial neural network based classifier

Validated differentiation of Listeria monocytogenes by FT-IR spectroscopy using an artificial neural network based classifier

Validated workflow for the differentiation of Listeria monocytogenes serogroups by FT-IR spectroscopy.

Differentiation of Listeria monocytogenes-FT-IR spectroscopy

In this training session, the METTLER TOLEDO product managers will address the questions that are most commonly asked in the field. They will talk about weight handling, maintenance of mass comparators, selection of products, elevation dependence of measurements, and many more topics. Additionally, you will have the opportunity to ask your own questions in an open Q&amp;A session at the end.
Key Takeaways
This is a great opportunity for all mass metrologists, as those questions that many others ask might also be of relevance to you. 
Program
<ul>
<li>Discover answers to the most frequently asked questions in mass metrology
</li>
<li>Learn more about weight handling, mass comparator maintenance, etc.
</li>
<li>Ask our experts your own questions
</li>
</ul>
<blockquote>
Speaker: Dr. Frederik Eiler (METTLER TOLEDO, Product Manager Metrology Solutions)
</blockquote>
<blockquote>
Speaker: Mark Kliebenschädel (METTLER TOLEDO, Product Manager Metrology Solutions)
</blockquote>

Mettler-Toledo: Mass Calibration – Most Frequently Asked Questions

Mass Calibration – Most Frequently Asked Questions

Opportunity for all mass metrologists, as those questions that many others ask might also be of relevance to you.

Laboratories are constantly trying to manage their efficiency. When it comes to ion chromatography, increasing the speed of the analysis is often the first thing that comes to mind to achieve this goal. But what are the tradeoffs?
In this webinar, we’ll take a dive into the influencing factors for chromatographic separation, what levers you have when trying to speed analysis up, what you gain and what you might give up for the compressed run time. We’ll try to help you decide where to draw the line for your lab while sharing some method examples that benefit from fast run times. Lastly, we’ll look at other strategies for efficiency like automated sample prep. 
Key Learning Points:
<ul>
<li>Learn about speeding up IC methods, including factors influencing chromatographic separation</li>
<li>Understand the trade-offs and things to consider when speeding up your analysis</li>
<li>Learn about alternatives to increase sample throughput when the tradeoff for speed is too much</li>
</ul>
Who Should Attend?
<ul>
<li>People developing IC methods </li>
<li>Anyone using ion chromatography</li>
<li>Lab managers and directors interested in strategies for faster lab results</li>
<li>Students and researchers looking to expand their fundamental understanding of IC</li>
</ul>
<blockquote>
Presenter: Bikash Chouhan, PhD (Applications Specialist, Ion Chromatography, Metrohm USA)
</blockquote>
<blockquote>
Bikash Chouhan is an Applications Specialist for Ion Chromatography at Metrohm USA. He completed his PhD in Analytical Chemistry from the University of Texas at Arlington (UTA). His area of research during his PhD was in Capillary Scale Ion Chromatography. He has worked as a Post Doc at Department of Chemistry and Biochemistry, UTA and as a Senior Scientist in IC at Eurofins, before joining Metrohm USA. He is currently involved in expanding IC applications to more unique and challenging sample matrices.
</blockquote>
<blockquote>
presenter: Linx Waclaski (Product Manager Ion Chromatography, Metrohm USA)
</blockquote>
<blockquote>
Linx Waclaski is the Product Manager for Ion Chromatography at Metrohm USA in Riverview, Florida. He has years of experience in multiple chromatographic techniques, including ion chromatography, gas chromatography, liquid chromatography, and mass spectrometry, with extensive experience in R&amp;D and applications development. His chromatography applications experience extends across environmental, food safety, and life sciences industries. He has an M.S. in Forensic Science from Duquesne University.
</blockquote>

Metrohm: Speeding up Your IC: Is Faster Always Better?

Speeding up Your IC: Is Faster Always Better?

Influencing factors for chromatographic separation, levers to speed analysis up, gains and losses of the compressed run time.

Per- and poly-fluorinated substances (PFAS) are synthetic organic compounds widely used in products and processes because of their hydrophobic and non-stick properties. Known as ‘forever chemicals’ due to their persistence in the environment, PFAS are identified as a class of environmental contaminants of high concern. Increasingly, environmental and regulatory agencies are taking action to address PFAS contamination, setting sub-parts-per-billion (ppb) tolerance thresholds to limit exposure and protect public health.
While targeted techniques can detect these compounds at low levels, the number of current targets is limited. Consequently, the use of non-targeted High Resolution Mass Spectrometry (HRMS) is becoming important for the analysis of PFAS. This is due to the rapid rate of detection of novel PFAS in samples and a lack of analytical standards.
Join us for these short two-part series (30 min) to take your PFAS analysis to the next level, where we will demonstrate the benefit of using HRMS workflows for advanced discovery, identification and quantitation of PFAS. 
Learn about recent LC developments and how the Xevo™ G3 QTof, and a SELECT SERIES™ Cyclic™ Ion Mobility Spectrometry (IMS) rise to this analytical challenge. 
Gain practical insights on workflows used by our scientists for your own PFAS investigations; see the range of inlet options available, how to run samples, experience the latest software for data acquisition and interpretation.
Register for:
Part 1 – 16th May (10:30-11:00 BST | 11:30-12:00 CEST)
LC developments and using Xevo G3 QTof for quantitation and screening of PFAS 
Part 2 – 23rd May (10:30- 11:00 BST | 11:30-12:00 CEST)
Advanced non-target approaches using Ion mobility
Key learning topics:
<ul>
<li>Recent developments in LC and why selection of column chemistries is critical for PFAS analysis </li>
<li>Learn about HRMS and IMS in the quest to discover new PFAS compounds and enhance confidence in identification of PFAS </li>
<li>See our chemists in action in the lab and gain practical insights on workflows</li>
</ul>
Who should attend?
<ul>
<li>Researchers and scientists actively addressing the PFAS issue using Liquid Chromatography or Mass Spectrometry.</li>
<li>PFAS Program Managers</li>
<li>Laboratory Managers</li>
</ul>
<blockquote>
Presenter: Stuart Adams (Consulting Scientist, Testing &amp; Lab Services, Food and Environmental, Waters Corporation)
</blockquote>
<blockquote>
Stuart started his career by attending the University of Glasgow studying for an honours degree in Food and Environmental Chemistry where his passion for solving analytical challenges started. He furthered his education with a doctorate from the University of Leeds in analytical chemist. Subsequently he spent 17 years working at Fera on a variety of projects looking at the detection of chemicals in challenging food and environmental samples using a variety of detection techniques. Whilst at Fera he has represented the UK at several European Union Reference Laboratory meetings for pesticide analysis and is a recognised international expert in his field. Stuart joined Waters Corporation in 2018 and currently works as a Consulting Scientist where he still loves to work on solving the analytical challenges that matter.
</blockquote>
<blockquote>
Presenter: Dr. Hania Khoury-Hollins (Principal Scientist, Waters Corporation)
</blockquote>
<blockquote>
Hania Khoury-Hollins is a scientist and researcher at Waters Corporation. She holds a bachelor’s degree in science, which laid the foundation for her scientific pursuits. In 2001, Hania earned her master’s degree in Toxicology, deepening her understanding of the impact of chemicals on living organisms. In 2005 she successfully defended her Ph.D. in physical chemistry from University René Descartes - Paris V (France). After undertaking several different projects in academia Hania spent some time working at Agilent before joining Waters.
</blockquote>
<blockquote>
At Waters, her work revolves around developing cutting-edge analytical techniques and their applications with a focus on Liquid Chromatography Coupled with High-Resolution Mass Spectrometry (LC-HRMS). Her expertise encompasses a diverse range of applications, including drug development, proteomics, metabolomics, and lipidomics. More recently, Hania has been developing applications strategies for per- and polyfluoroalkyl substances (PFAS).
</blockquote>

Waters Corporation: Take your PFAS Analysis to the Next Level (1) with Advanced MS for Non-Targeted Analysis

LC developments and using Xevo G3 QTof for quantitation and screening of PFAS

Learn about HRMS and IMS in the quest to discover new PFAS compounds and enhance confidence in identification of PFAS.

LC developments and using Xevo G3 QTof for PFAS

Whether the goal is to obtain enough intermediate for the next step in a synthetic scheme or to polish a final product, preparative liquid chromatography is one of the primary techniques for isolation compounds from complex mixtures. Despite this fact, purification often seems difficult with many opportunities for undesirable outcomes, such as poor compound purity, low yield, or target loss. All these unfortunate results require time-consuming rework, a detriment to the all-to-familiar compressed timelines evident in so many workplaces today.
The uncertainty and fear associated with scaling up a separation can be mitigated by adopting simple suggestions which help to ensure success. Practical and easily implemented tips for predictable scaleup and successful compound isolation will be the focus of this webinar.
<h5>Key Learning Objectives:</h5>
<ul>
<li>Learn to scale your analytical – prep methods predictably.</li>
<li>Gain knowledge from practical guidance shared for first-time purification success when scaling your LC separation.</li>
<li>Discover how following the rules of scaling PREP LC simplifies the process</li>
<li>Learn how to better control LC scale-up outcomes and boost both target purity and recovery.</li>
</ul>
<blockquote>
Presenter: Jo-Ann Jablonski (Principal Scientist, Waters Corporation)
</blockquote>
<blockquote>
Jo-Ann Jablonski is a Principal Scientist in the Consumables and Lab Automation Group at Waters Corporation. Prior to joining Waters, she worked in biotechnology synthesizing, characterizing, and purifying biomolecules for research and development. She later transitioned to developing a process for the manufacture of a therapeutic peptide product. Since joining Waters, Jo-Ann has provided technical support, training, and application assistance to customers using Waters’ chemistry products and instrumentation, with an emphasis on purification technologies.
</blockquote>

Waters Corporation: Tips for Getting to Predictable Scaleup in Prep LC

Tips for Getting to Predictable Scaleup in Prep LC

Practical and easily implemented tips for predictable scaleup and successful compound isolation.

The new Thermo Scientific Orbitrap Astral mass spectrometer is setting a new standard for mass spectrometry performance, redefining what is possible and unlocking additional insights into biology and disease mechanisms.
The ability to conduct large cohort protein analysis with faster throughput, deeper coverage, higher sensitivity and accurate and precise quantitation enables a better understanding of the dynamic, temporal and spatial complexities of biology.
In this interactive webinar, we will briefly introduce underlying technology of the Orbitrap Astral mass spectrometry platform before opening the floor to a group of renowned cancer researchers who will describe their own early experiences with the system and their hopes and ambitions for advancing their own researches in this important and rapidly-advancing field.
As a participants in this webinar, you will have the opportunity to engage in an interactive panel discussion with these key opinion leaders and ask questions to further enhance your understanding of the current and future state of mass spectrometry-driven proteome analysis of cancer samples and how that can identify differentially expressed proteins, protein-protein interactions and post-translational modifications that may be associated with cancer development, progression, or response to treatment.
What you will learn:
<ul>
<li>View the latest research in protein profiling</li>
<li>Learn about the Orbitrap mass spectrometry solutions</li>
<li>Discover how your cancer research can be empowered</li>
</ul>
Who should attend?
<ul>
<li>Scientific researchers in the field of cancer proteomics</li>
<li>Mass spectrometrists working in core labs in cancer institutes</li>
<li>Laboratory managers in cancer institutes</li>
</ul>
If you have questions for our experts, please submit them during registrations or send them anytime in advance via email to analyze.eu@thermofisher.com
Live questions will be handled during the webinar in our Q&amp;A chat. 
Short notice: Can&#39;t attend the live broadcast? No problem! Register anyway and you will automatically receive the link to the webinar recording.
<blockquote>
Presenter: Dr. Christian Hock (Senior Manager Instrument Development, Thermo Fisher Scientific)
</blockquote>
<blockquote>
The Orbitrap Astral development team is led by Dr. Christian Hock, who joined the Research team of Dr. Alexander Makarov as Lead Scientist in early 2013. With a strong passion for science and an innate curiosity, Dr. Hock has been involved in all aspects of Astral technology development, from its initial stages of basic research to its successful transition into serial production.
</blockquote>
<blockquote>
In 2017, Dr. Hock transferred to the Bremen R&amp;D organization, where he took the initiative to establish a dedicated R&amp;D team which has been instrumental in bringing the Orbitrap Astral product to fruition. His overarching goal is to exceed customer expectations by delivering cutting-edge products that enable researchers worldwide to accelerate their scientific output. Through his leadership and expertise, he continuously pushes the boundaries of science &amp; technology and strives for excellence in every stage of product development.
</blockquote>
<blockquote>
Presenter: Prof. Michiel Vermeulen (Senior Group Leader, Netherlands Cancer Institure NKI)
</blockquote>
<blockquote>
Presenter: Dr. Harmjan Vos (Head of the Proteomics Facility, University Medical Centre Utrecht)
</blockquote>
<blockquote>
Started his proteomics career as a postdoc in a project of Jeroen Krijgsveld in the lab of Albert Heck at the UU. Then he moved to the lab of Boudewijn Burgering at the Center for Molecular Medicine (CMM) at the UMC Utrecht, trying to understand the intricate connections between cellular signaling pathways and metabolic processes. Since 2014 he is heading the Proteomics facility at CMM, which is involved in the research infrastructure of the Oncode Institute, with an emphasis on single cell proteomics.
</blockquote>
<blockquote>
Presenter: Prof. Jyoti Choudhary (Group Leader, The Institute of Cancer Research)
</blockquote>
<blockquote>
Professor Jyoti Choudhary&#39;s research focuses on the development and implementation of novel mass spectrometry and data analysis approaches for proteome discovery. She is Head of the Proteomics Core Facility and Career Faculty Leader of the Functional Proteomics Research Group.
</blockquote>
<blockquote>
Presenter: Dr. Joanna Kirkpatrick (Technical Sales Specialist, Proteomics, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Jo has a degree in Chemistry from the University of York and a PhD in analytical chemistry from the University of Reading. Following a post-doc in mass spectrometry in the Chemistry department at Oxford, and working as an application scientist at Waters, she has worked in and ran Proteomics Core Facilities in the UK and Germany, including the EMBL in Heidelberg, the Leibniz Institute on Aging in Jena, The Francis Crick Institute in London and CRUK Cambridge.
</blockquote>
<blockquote>
After many years as an experienced Thermo customer, Jo joined the company last year as a Technical Sales Specialist, supporting the whole of the UK/Ireland, Benelux and Nordic regions, in all things proteomics.
</blockquote>
<blockquote>
When she&#39;s not talking about proteomics and mass spectrometry, Jo enjoys ceroc dancing, tennis, scuba diving, travelling, and spending time with her cats, who like to join in on Teams meetings whenever she works from home.
</blockquote>

ThermoFisher Scientific: High throughput protein profiling for cancer research by Orbitrap Astral MS

High throughput protein profiling for cancer research by Orbitrap Astral MS

Cancer research by Orbitrap Astral MS

<h4>Virtual event: Pharma conclave series 2024</h4>
SCIEX offers the platform for the experts to take the stage and drive technological progress
Welcome to Pharma conclave series 2024 – where our customers’ expertise meets innovation, transforming your knowledge and empowering you to revolutionize your work processes. Join us in discovering techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.
From the research of new markers to the drug development moving into non and GLP bioanalysis. Confidently plan your next lab expansion with the knowledge gained from industry leaders and pioneers.
<ul>
<li>May 16 Event 1: Pharma omics</li>
<li>September 17 Event 2: Impurities</li>
<li>October 23-24 Event 3: Pharma</li>
</ul>
10:00 a.m.–12:00 p.m. CET
<h3>Event 1: Pharma omics</h3>
Lost in translation? Translational research meets drug discovery and development – 3rd edition
<h5>Topics covered:</h5>
<ul>
<li>‘Pathophysiological subtypes of mild cognitive impairment due to Alzheimer’s disease identified by CSF proteomics’ by Bruno Manadas, at CNC
</li>
<li>‘Scalable and automated workflows for global plasma proteomics by standardized LC-MS’ by Dorte Bekker-Jensen, at Evosep
</li>
<li>‘Quantitative, standardized and reproducible metabolomics solutions in pharmaceutical R&amp;D’, by Stefan Ledinger and Markus Langsdorf, at Biocrates Life sciences
</li>
<li>Multi-omics analysis on mouse plasma in a pre clinical development of a drug in Alzheimer
</li>
</ul>
<blockquote>
Presenter: Bruno Manadas, PhD
</blockquote>
<blockquote>
Dr Manadas is a prominent Cell Biology researcher at CNC-UC since 2017. With expertise in biochemistry, psychology, and biotechnology, he&#39;s authored 130+ publications and led multiple projects. As head of CNC&#39;s Mass Spectrometry node and Associate Editor for Frontiers, he&#39;s contributed significantly to neuroscience and psychiatry. Bruno&#39;s research focuses on cell biology mechanisms, translational biomarker research, and MS-based approaches for studying protein oxidation. He played a pivotal role in establishing the hospital biobank and teaches at the Portuguese Catholic University.
</blockquote>
<blockquote>
Presenter: Dorte Bekker-Jensen
</blockquote>
<blockquote>
Dorte is heading the Applications Development team in Proteomics Research at Evosep. She has long-term experience within proteomics and has focused on technology development by developing simple and automated workflows for high-throughput applications.
</blockquote>
<blockquote>
Presenter: Stefan Ledinger
</blockquote>
<blockquote>
Stefan has 20 years of experience in the life sciences and diagnostic industries. For the last dozen years, he has been serving various functions within biocrates life sciences ag. Most recently, he has been responsible for the market development for metabolomics solutions in the pharmaceutical and biotech industries. He currently serves as Global Head of Sales. Stefan holds degrees in business administration and bioengineering, combining commercial know-how with a passion for science.
</blockquote>
<blockquote>
Presenter: Markus Langsdorf
</blockquote>
<blockquote>
Markus has 14 years of experience in life sciences industries. As he joined biocrates in 2010, he worked for 11 years as application support specialist for biocrates’ metabolomics profiling solutions. 3 years ago he changed his position withing the company to his new function as product manager. Markus studied chemistry and graduated with a PhD in Analytical Chemistry.
</blockquote>

Pharma conclave series 2024: Pharma omics

Discover techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.