What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

Article

Application

Academy

Popularization

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Tosoh Bioscience

Metrohm

Agilent Technologies

Technology Networks

SelectScience

C&EN

SCIEX

Waters Corporation

Per- and poly-fluorinated substances (PFAS) are synthetic organic compounds widely used in products and processes because of their hydrophobic and non-stick properties. Known as ‘forever chemicals’ due to their persistence in the environment, PFAS are identified as a class of environmental contaminants of high concern. Increasingly, environmental and regulatory agencies are taking action to address PFAS contamination, setting sub-parts-per-billion (ppb) tolerance thresholds to limit exposure and protect public health.
While targeted techniques can detect these compounds at low levels, the number of current targets is limited. Consequently, the use of non-targeted High Resolution Mass Spectrometry (HRMS) is becoming important for the analysis of PFAS. This is due to the rapid rate of detection of novel PFAS in samples and a lack of analytical standards.
Join us for these short two-part series (30 min) to take your PFAS analysis to the next level, where we will demonstrate the benefit of using HRMS workflows for advanced discovery, identification and quantitation of PFAS. 
Learn about recent LC developments and how the Xevo™ G3 QTof, and a SELECT SERIES™ Cyclic™ Ion Mobility Spectrometry (IMS) rise to this analytical challenge. 
Gain practical insights on workflows used by our scientists for your own PFAS investigations; see the range of inlet options available, how to run samples, experience the latest software for data acquisition and interpretation.
Register for:
Part 1 – 16th May (10:30-11:00 BST | 11:30-12:00 CEST)
LC developments and using Xevo G3 QTof for quantitation and screening of PFAS 
Part 2 – 23rd May (10:30- 11:00 BST | 11:30-12:00 CEST)
Advanced non-target approaches using Ion mobility
Key learning topics:
<ul>
<li>Recent developments in LC and why selection of column chemistries is critical for PFAS analysis </li>
<li>Learn about HRMS and IMS in the quest to discover new PFAS compounds and enhance confidence in identification of PFAS </li>
<li>See our chemists in action in the lab and gain practical insights on workflows</li>
</ul>
Who should attend?
<ul>
<li>Researchers and scientists actively addressing the PFAS issue using Liquid Chromatography or Mass Spectrometry.</li>
<li>PFAS Program Managers</li>
<li>Laboratory Managers</li>
</ul>
<blockquote>
Presenter: Stuart Adams (Consulting Scientist, Testing &amp; Lab Services, Food and Environmental, Waters Corporation)
</blockquote>
<blockquote>
Stuart started his career by attending the University of Glasgow studying for an honours degree in Food and Environmental Chemistry where his passion for solving analytical challenges started. He furthered his education with a doctorate from the University of Leeds in analytical chemist. Subsequently he spent 17 years working at Fera on a variety of projects looking at the detection of chemicals in challenging food and environmental samples using a variety of detection techniques. Whilst at Fera he has represented the UK at several European Union Reference Laboratory meetings for pesticide analysis and is a recognised international expert in his field. Stuart joined Waters Corporation in 2018 and currently works as a Consulting Scientist where he still loves to work on solving the analytical challenges that matter.
</blockquote>
<blockquote>
Presenter: Dr. Hania Khoury-Hollins (Principal Scientist, Waters Corporation)
</blockquote>
<blockquote>
Hania Khoury-Hollins is a scientist and researcher at Waters Corporation. She holds a bachelor’s degree in science, which laid the foundation for her scientific pursuits. In 2001, Hania earned her master’s degree in Toxicology, deepening her understanding of the impact of chemicals on living organisms. In 2005 she successfully defended her Ph.D. in physical chemistry from University René Descartes - Paris V (France). After undertaking several different projects in academia Hania spent some time working at Agilent before joining Waters.
</blockquote>
<blockquote>
At Waters, her work revolves around developing cutting-edge analytical techniques and their applications with a focus on Liquid Chromatography Coupled with High-Resolution Mass Spectrometry (LC-HRMS). Her expertise encompasses a diverse range of applications, including drug development, proteomics, metabolomics, and lipidomics. More recently, Hania has been developing applications strategies for per- and polyfluoroalkyl substances (PFAS).
</blockquote>

Waters Corporation: Take your PFAS Analysis to the Next Level (2) with Advanced MS for Non-Targeted Analysis

Advanced non-target approaches (PFAS) using Ion mobility

Learn about HRMS and IMS in the quest to discover new PFAS compounds and enhance confidence in identification of PFAS

One of the key aspects of water quality assurance is monitoring disinfection byproducts (DBPs), especially haloacetic acids (HAAs), bromate, and dalapon.
As these substances pose a significant health risk, regulatory bodies across the world, such as the US EPA and the European Union, have established strict guidelines to limit their presence in drinking water. The EPA Method 557 by the US EPA standardizes the comprehensive monitoring of 9 HAAs, bromate, and dalapon.
This webinar will discuss the latest advancements in analytical techniques and instrumentation that meet the regulatory requirements. The focus will be on exploring the capabilities of ion chromatography coupled with mass spectrometry (IC-MS and IC-MS/MS), a method that is highly sensitive, reliable, and efficient in detecting low levels of HAAs and other DBPs without requiring extensive sample preparation.
Attendees will have the opportunity to witness the seamless integration of HAA analysis by IC-ESI-MS/MS and IC-ESI-MS within water laboratories, demonstrating consistent results and efficient lab processes. Our experts will showcase how ion chromatography and mass spectrometry provide a streamlined workflow, from sample injection to analysis, significantly reducing manual labor and associated costs. 
<h5>Key learnings of this webinar:</h5>
<ul>
<li>Learn how IC coupled to mass spectrometry is the ideal and most reliable technique for analysis of HAA, bromate, and dalapon in drinking water</li>
<li>Develop an understanding of how ion chromatography and mass spectrometry can complement each other to enhance selectivity and sensitivity</li>
<li>Learn to know Metrohm and Agilent as your reliable lab partners</li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Water utility operators who need robust and dependable analytical techniques to guarantee the safety of drinking water</li>
<li>Scientists, researchers, and analytical method developers who need to stay up-to date on state-of-the-art methods for water analysis</li>
<li>Laboratory staff and managers considering to implement reliable, cost- and time-efficient analysis of HAA in their lab</li>
</ul>

Metrohm: Water safety: Improve your analysis of haloacetic acids with IC-ESI-MS/MS

Water safety: Improve your analysis of haloacetic acids with IC-ESI-MS/MS

The latest advancements in analytical techniques that meet the regulatory requirements in monitoring disinfection byproducts (DBPs).

Improve your analysis of haloacetic acids with IC-ESI-MS/MS

<h5>Overview:</h5>
<ul>
<li>The reporting limit of a method is also dependent upon the consistency of clean blanks and elimination of sporadic contamination.</li>
<li>Vendors of PFAS consumables need to provide certified, trace level PFAS free products.</li>
<li>PFAS are everywhere! They are widely used, vendors/suppliers when contacted don&#39;t know their processes are using them.</li>
<li>Since PFAS contamination has become an issue with PFAS analysis all field samples are taken in duplicate, not just field blanks. </li>
<li>The complete data package and replicates need to be evaluated by the user in order to make sound conclusions and identify false positives. </li>
<li>Every step there is a chance to incorporate “free-range” PFAS in your sample, minimize the preparation steps</li>
<li>How PFAS is analyzed at EPA Region 5 Laboratory</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Anyone lab scientists, or managers involved in PFAS Analysis</li>
</ul>
<h5>Key Learning Points:</h5>
<ul>
<li>Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be</li>
<li>Become aware of Sporadic PFAS Contamination</li>
<li>Understand how to identify false positives</li>
<li>Require all analysis quality control and samples to be analyzed in replicates to identify false positives.</li>
</ul>
<blockquote>
Presenter: Larry Zintek (Organic Methods Group Leader, UA EPA)
</blockquote>
<blockquote>
Presenter: Maryam Sayeed (Product Management Specialist, Phenomenex)
</blockquote>

Phenomenex: Identifying and Avoid False Positives During Routine PFAS Analysis

Identifying and Avoid False Positives During Routine PFAS Analysis

Learn how to approach analyzing for PFAS, it is not rocket science like they made it out to be.

False Positives During Routine PFAS Analysis

<h4>LC-MS/MS fundamentals webinar series 2.0</h4>
Advanced techniques for data acquisition and processing
Elevate your analytical expertise with advanced techniques and workflows
Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series. Based on our LC-MS/MS 1.0 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows.
Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.
<h4>This webinar:</h4>
May 21, 2024 11:00 a.m. - 11:30 a.m. CET - Advanced MS/MS method building 
Gain insight into advanced features for maximizing sensitivity in your mass spectrometry analyses.
<h4>Other webinars from the series:</h4>
May 7, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3820">Beyond the fundamentals</a>
June 4, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3853">Mastering chromatography</a>
Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.
June 18, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3854">It&#39;s a TRAP! Getting the most out of your QTRAP system</a>
Learn when to utilize QTRAP functionality to create more selective and sensitive methods. July 2, 2024
11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3855">Troubleshooting at the source</a>
Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.
July 16, 2024 11:00 a.m. - 11:30 a.m. CET <a href="https://lcms.labrulez.com/webinar/3856">Automating data processing</a>
Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.
July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3857">Excel strategies for mass spectrometry data analysis</a>
Take an in-depth look at when and how to use Excel to increase your productivity.

SCIEX: LC-MS/MS fundamentals webinar series 2.0

Advanced MS/MS method building

Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series.

In this webinar, Dr. Qi Huang and John D. Laycock from Tecan will present AffinEx’s Protein A Capture – a new protein A-bound sorbent for antibody purification with an affinity for IgG and monoclonal antibodies (mAbs). They will also highlight the various applications and workflows that can be accomplished using AffinEx Protein A Capture.
Tecan’s protein A sorbent ships and stores in a dry state eliminating the need for environmental controls with a 24-month shelf life under ambient conditions. Explore how Tecan can leverage positive pressure processing for consistent and efficient extraction protocols.
Key learning objectives
<ul>
<li>Explore AffinEx’s poly(methyl methacrylate) (PMMA) matrix</li>
<li>Learn more about Narrow Bore Extraction™ (NBE™) formatting</li>
<li>Explore applications for protein A</li>
<li>Gain insights into workflow solutions using Tecan instruments and consumables</li>
</ul>
Who should attend?
Vaccine researchers, those working in proteomics, biopharma, and CROs, and anybody conducting antibody researchProtein purification/research, Antibodies, Biopharma, CRO, clinical diag., bioprocessing, fermentation process dev., clone screening and selection
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. John D. Laycock, Ph.D. (Tecan)
</blockquote>
<blockquote>
John D. Laycock joined Amgen in 1998 where he setup of the first small molecule GLP bioanalytical lab. While at Amgen, he implemented laboratory automation, LIMS, and informatics to holistically streamline workflows for bioanalytical sample analysis and reporting throughout all stages of drug development. In 2015 he joined SPEware which was acquired by Tecan in 2016. With Tecan he has been responsible for launching the Resolvex A200 and various chemistry consumable products into the global marketplace.
</blockquote>
<blockquote>
Presenter: Dr. Qi Huang (Tecan)
</blockquote>
<blockquote>
As a medicinal chemist, Dr. Qi Huang spent 17 years in drug discovery at Amgen and other organizations searching for novel therapeutics. He moved to Tecan nine years ago, where he has led many product and method development projects providing sample preparation solutions for LC-MS based clinical, forensic, and toxicological tests. Recently, he has joined the chemistry R&amp;D team where he leads consumable product development that includes AffinEx Protein A, a new product line developed for the biopharma industry.
</blockquote>
<blockquote>
Presenter: Will Thompson (SelectScience)
</blockquote>
<blockquote>
Will studied Biology at the University of Bristol, UK, and as a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.
</blockquote>

AffinEx Protein A Capture

AffinEx’s Protein A Capture – a new protein A-bound sorbent for antibody purification with an affinity for IgG and monoclonal antibodies (mAbs).

The successful regulatory filing of any pharmaceutical product development dossier relies on scientific and rational way of analytical method development.
There are many analytical development strategies which can be followed like “Traditional” or “Enhanced approach”. Enhanced Approach as stated by ICH, or DoE based Quality-by-Design (QbD) approach are one of such method development strategies which will be discussed in webinar. These approaches are now part of regulations across USP, ICH and USFDA. We will also discuss the different concepts like ATP, Lifecycle Management, Risk Assessment etc. Join us for this webinar to understand different guidelines related to analytical development and how can you adopt QbD or Enhanced approach in the analytical method development.
<blockquote>
Presenter: Manu Grover (Business Development Manager – Pharma Markets, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Manu Grover holds a postgraduate degree in Pharmacy from Manipal Academy, India. He has more than 21 years of chromatography experience and has worked in pharmaceuticals to support IND, NDA, and ANDA filings incompanies such as Dr. Reddy&#39;s, Panacea Biotec, and Ozone Pharmaceuticals. During his professional journey, he has developed and validated analytical methods on HPLC, GC, particle sizing, DSC, pXRD, and LC/MS instruments, as well as successfully filing multiple analytical dossiers in U.S./EU markets. In his current role, he works closely with the Agilent sales team, regulators, and pharmaceutical scientists. He shares market updates, trends, and expertise related tothe Agilent portfolio of columns and supplies.
</blockquote>

Agilent: Becoming a Better Chromatographer - Educational Webinar Series

Regulatory Guidelines on Analytical Method Development: USFDA, ICH and USP Perspective

Understand different guidelines related to analytical development and how can you adopt QbD or Enhanced approach in the analytical method development.

Regulatory Guidelines on Analytical Method Development

New for 2024, this online event will distill complex scientific concepts into concise, informative 10-minute presentations. Our expert panel of speakers will cover sources, impacts and mitigation strategies for environmental pollutants, providing a comprehensive overview of the challenges and solutions in this highly topical field.
<h5>This online event will explore:</h5>
<ul>
<li>The impact and emission of particles from road vehicles</li>
<li>Global concerns and persistence of poly- and perfluorinated alkyl substances</li>
<li>Sample preparation for the analysis of environmental pollutants</li>
<li>The environmental impacts of synthetic chemicals</li>
<li>Environmental pollutants and media in our everyday space</li>
</ul>
<h5>17:00 – 17:15 The Environmental Impacts of Synthetic Chemicals</h5>
<ul>
<li>Alistair Boxall, PhD (University of York)</li>
</ul>
<h5>17:10 – 17:25 Talk Title to be Confirmed</h5>
<h5>17:25 – 17:35 The Problem of PFAS Here, PFAS There, PFAS Everywhere</h5>
<ul>
<li>Rainer Lohman, PhD (University of Rhode Island)</li>
</ul>
<h5>17:35 – 17:45 Creating the Right Environment for Indoor Air Quality</h5>
<ul>
<li>Greg Davies (Assurity Consulting)</li>
</ul>
<h5>17:45 – 17:55 Environmental Pollutants and Media in Our Everyday Space</h5>
<ul>
<li>Jamie Mitri (Moss Pure)</li>
</ul>
<h5>17:55 – 18:05 Are Electric Vehicles Zero Emissions?</h5>
<ul>
<li>David Green, PhD (Imperial College London)</li>
</ul>
<h5>18:05 – 18:15 Sample Preparation for the Analysis of Environmental Pollutants</h5>
<ul>
<li>Peter Baugh, PhD (The BMSS)</li>
</ul>
<h5>18:15 Event Close</h5>
<blockquote>
Presenter: Greg Davies (Director of Market Development, Assurity Consulting)
</blockquote>
<blockquote>
Greg has worked for over 35 years in health, safety and environmental compliance management covering aspects including Legionella, H&amp;S, fire, asbestos, building environment quality, energy, waste and sustainability. A degree-qualified microbiologist and registered expert witness, he joined Assurity Consulting in 1989. Greg is a Fellow of the Royal Society of Public Health, Specialist Member of the International Institute of Risk and Safety Management (IIRSM), is a committee member of the IWFM Sustainability Special Interest Group and Chair’s the IIRSM London Branch committee. Greg is a regular blogger and contributor to our industry press, with over 250 articles published on environmental management/sustainability, health and safety and risk. He chaired the CIBSE committee that revised TM13, contributed to CIBSE Guides A and M, was the author of the original IWFM good practise guide to risk management and has been survey lead for the IWFM sustainability in FM survey. On subjects ranging from water management to the “Red Tape Challenge” Greg has also spoken at events for, or been involved with, organisations including Defra, PHE, IIRSM, DECC, BSC, RICS, CIEH, BSRIA, CIBSE, IWFM, IOSH, CIEH and the London Health and Safety Group.
</blockquote>
<blockquote>
Presenter: Jamie Mitri (Founder and CEO, Moss Pure)
</blockquote>
<blockquote>
Jamie Mitri is an Entrepreneur with degrees in Chemical Engineering and Biology. Jamie spent her career developing and manufacturing medical devices and biotechnology products used in the ICU of hospitals, overseeing environmental compliance, including air pollution and wastewater, and more recently as a project manager focusing on business strategy and marketing. Jamie founded her company, Moss Pure, at a startup competition at the Massachusetts Institute of Technology, where it won First Place Startup (MIT Lebanon Challenge). Jamie then participated in the United Nations Development Programme, where Moss Pure won Top 10 Startup. Most moss companies use preserved or dried moss which is no longer living. Moss Pure is the world&#39;s only live moss air filter and stress relief device, while acting as an aesthetically pleasing decor piece in your home and office. No watering, sunlight, or maintenance is needed as Moss Pure&#39;s proprietary engineering sustains the live moss with the product itself, working in synergy to allow moss to obtain its nutrients from the air and become an air filter. Jamie uses both her engineering and science skills in the upstream process of the assembly of each piece, where the multi-layered air filter is built, and then uses her love and skill in fashion and interior design in the downstream process to produce a unique, aesthetically pleasing art piece for your space. For more information, feel free to contact Jamie and visit www.mosspure.com.
</blockquote>
<blockquote>
Presenter: Peter J Baugh, BSc, PhD, FRSC (Co-leader of the Environmental and Food Analysis Special Interest, The BMSS)
</blockquote>
<blockquote>
BSc (Hons., 1961) and PhD (1964), University of Wales, retired from the University of Salford, Department of Chemistry and Applied Chemistry, 1967 – 2000. Currently, co-leader of the environmental and food analysis special interest of the BMSS, past visiting professor at the University of Chester, life member of the BMSS for services to mass spectrometry.
</blockquote>
<blockquote>
Presenter: Aaron Hineman (Inorganic Product Line Leader, PerkinElmer)
</blockquote>
<blockquote>
Aaron Hineman is Americas Inorganic Product Line Leader at PerkinElmer. He is a skilled speaker and trainer with over 20 years of expertise in inorganic analytical chemistry. He spent a decade developing analytical methodologies for international operations. Aaron&#39;s extensive knowledge spans the implementation of atomic spectroscopic technologies, including ICP-MS and ICP-OES instrumentation, as well as hyphenated speciation techniques. 
</blockquote>
<blockquote>
Presenter: Rainer Lohmann, PhD (Professor of Oceanography, Director of the URI Superfund Center on PFAS, University of Rhode Island)
</blockquote>
<blockquote>
Dr. Rainer Lohmann is a Professor at the University of Rhode Island’s Graduate School of Oceanography. He obtained a degree in Chemical Engineering and a Doctorate in Environmental Science. He is Director of the URI-led Superfund Research Program Center on the Sources, Transport, Exposure and Effects of PFAS (STEEP). His research focuses on the detection, bioaccumulation, transport and fate of anthropogenic pollutants in the environment, often relying on passive samplers in the process. Since 2000, he has published &gt; 180 publications and book chapters. Dr. Lohmann was member of the U.S. EPA’s Board of Scientific Counselors (2017-2023) and serves as Editor of Environmental Toxicology and Chemistry. He was awarded a Fulbright Fellowship as part of the Fulbright Arctic Initiative III in 2020, and an Alexander-von-Humboldt Fellowship in 2011.
</blockquote>
<blockquote>
Presenter: David Green, PhD (Senior Research Fellow, Imperial College London)
</blockquote>
<blockquote>
Dr David Green is a Senior Research Fellow in the Environmental Research Group where he leads the Aerosol Science Team. Research interests focus on the measurement of particles and gases, and their use in source apportionment and health studies, to improve our understanding in both the urban and global environment. Source apportionment methodologies are used to interpret the high time resolution chemical composition measurements and quantify the contribution of different emission sources in complex urban environments. In these environments a wide variety of sources, both local (such as traffic) and distant (such as that from agricultural sources), contribute to the total particle load in the atmosphere.
</blockquote>
<blockquote>
Presenter: Alistair Boxall, PhD (Alistair Boxall, PhD, Professor of Environmental Science, University of York)
</blockquote>
<blockquote>
Alistair Boxall is Professor in Environmental Science in the Environment Department and Director of the NERC-funded ECORISC Centre for Doctoral Training. Alistair’s research focuses on understanding emerging and future ecological and health risks posed by chemical contaminants in the natural environment.
</blockquote>

Technology Networks: Teach Me in 10: Environmental Pollutants

Teach Me in 10: Environmental Pollutants

Sources, impacts and mitigation strategies for environmental pollutants, providing a comprehensive overview of the challenges and solutions.

HPLC is a powerful analytical technique widely used in the food industry, research labs and commercial testing labs for quality control, safety assessment, and nutritional analysis. It is particularly valuable for analyzing compounds such as vitamins, amino acids, sugars, oils and toxins like mycotoxins. HPLC plays a crucial role in ensuring the safety, quality, and nutritional integrity of food products. Its ability to provide detailed information about the composition of food samples makes it an indispensable tool for food testing laboratories and the food industry.
Altium is happy to announce webinar on Agilent LC solutions for food industry. Reserve your spot now!
<a href="https://events.teams.microsoft.com/event/ecf82ef6-1be9-404d-8dda-732d993c8699@3a2a17c9-a748-438b-8c0e-b375b3713c75">REGISTRATION</a>
Key learning points:
<ul>
<li>HPLC introduction.</li>
<li>Key HPLC features which can reduce human error and enhance efficiency.</li>
<li>HPLC applications for vitamins analysis.</li>
<li>HPLC applications for amino acid analysis using automated pre column derivatization method.</li>
<li>HPLC application for sugar analysis.</li>
<li>HPLC application for aflatoxin analysis.</li>
<li>GPC as a technique of sample prep for removing high molecular matrix.</li>
</ul>
There will be a Q&amp;A session with the support of local Altium teams.
Looking forward to meeting you.
<blockquote>
Speaker: Laura Montis (IDO Product Specialist LPS Agilent Technologies)
</blockquote>

Altium International: Agilent LC Solutions For Food Testing and Food Fortification Webinar

Agilent LC Solutions For Food Testing and Food Fortification Webinar

HPLC is widely used in the food industry, research labs and commercial testing labs for quality control, safety assessment, and nutritional analysis.

Agilent LC Solutions For Food Testing and Food Fortification

UV-Vis spectroscopy is a mature technology used to analyze, characterize, and quantify pharmaceutical and biological samples such as active pharmaceutical ingredients, DNA/RNA, and proteins for many decades. The use of UV-Vis has been limited by the workflow needed to make these measurements efficiently. Recent advances in UV-Vis spectroscopy focus on enhancing lab productivity, offering ease of use, and providing multiple accessories designed specifically for application needs. Pharmaceutical and biopharmaceutical materials have become more sophisticated in life science research and the technology used for analysis should evolve too. This webinar will highlight the new Agilent Cary 3500 Flexible UV-Vis spectrophotometer and its capabilities in improving workflows in the pharmaceutical industry.
<h5>Key Learning Objectives:</h5>
<ul>
<li>Use of the double beam Cary 3500 Flexible UV-Vis spectrophotometer for analyzing liquid and solid samples</li>
<li>See how wide linear dynamic range allows direct measurement of highly absorbing samples, minimizing sample prep time and increasing efficiency and accuracy.</li>
<li>Use of a variable pathlength cell holder to measure impurities in ethanol as outlined in pharmacopeias (UPS, EP, and JP)</li>
<li>Use of a solid sample accessory kit to determine spectral transmission measurements of plastic pharmaceutical containers in accordance with USP &lt;671&gt;.</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Pharma/biopharma QA/QC laboratory analysts and laboratory managers</li>
<li>Chemical and Energy laboratory analysis and laboratory managers</li>
<li>Academia</li>
<li>Scientist and post-graduates</li>
</ul>
<blockquote>
Presenter: Geethika Weragoda (Application Scientist, Agilent Technologies Australia Pty Ltd)
</blockquote>

C&EN: Improve Your Pharmaceutical Workflows with Innovative UV-Vis Spectroscopy

Improve Your Pharmaceutical Workflows with Innovative UV-Vis Spectroscopy

This webinar will highlight the new Agilent Cary 3500 Flexible UV-Vis spectrophotometer.

Pharmaceutical Workflows with UV-Vis Spectroscopy

Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.
Dr. Carsten Arlt, Application Scientist at Tosoh Bioscience and Patrick Endres, Manager - Product Management EMEA, will explain the advantages of a novel Protein A chromatography resin for the purification of antibodies in batch and continuous applications. They will give an overview of the requirements and advancements in Protein A chromatography, show how a mAb purification process can be transferred from batch to continuous chromatography and what advantages this offers.
After the webinar, ask your questions during the live Q&amp;A session.
<blockquote>
Presenter: Christian Rohrer (Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH)
</blockquote>

Tosoh Bioscience: Tosoh Tuesday - Advances in mAb Purification from Batch to Continuous

Advances in mAb Purification from Batch to Continuous

The advantages of a novel Protein A chromatography resin for the purification of antibodies in batch and continuous applications.