What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Join us on Thursday, May 16th Online or in Dättwil - Switzerland to share your knowledge and challenges with peers and E&amp;L experts. As an attendee you will have the possibility to network, build useful relationships, and hear panel discussions.
<h4>Venues</h4>
Attend On-site at Waters AG | Täfernstrasse 14a | 5405 Dättwil | Switzerland
OR
Join us Online via Zoom
<h4>Registration information:</h4>
<ul>
<li>Online participation via Zoom - please register here: <a href="https://waters.zoom.us/webinar/register/1817098039303/WN_-kIHnJLqSoeTIMiO-rgmRA">Zoom event</a>
</li>
<li>On-site participation - Contact us via email for participation in Dättwil - Seats are limited! To register please contact <a href="Laura_Venezia@waters.com">Laura_Venezia@waters.com</a> and specify your first name, last name, company, email, phone and country
</li>
</ul>
<h4>This years TOPICS:</h4>
<ul>
<li>Latest developments in analytical instrumentation solutions.</li>
<li>Analytical consequences for recyclers and food packaging producers of the EU Regulation 2022/1616 on recycled plastic materials and articles intended to come into contact with foods, Deadline 15. Sept 2024</li>
<li>PFAS as a NIAS – the analytical challenges</li>
<li>Panel discussion with Q &amp; A</li>
<li>Sharing knowledge and challenges with peers and E&amp;L experts</li>
<li>... and more</li>
</ul>
<h4>Preliminary Agenda</h4>
<h5>08:30 - 09:00 | For attendees in Baden-Dättwil - Welcome Coffee/Tea/Gipfeli</h5>
<h5>09:00 - 09:15 | Welcome and Introduction to the workshop - Jürgen Marquardt, BASF Schweiz AG &amp; Jens Jacobsen, Waters Schweiz AG</h5>
<h5>09:15 – 09:45 | Regulatory aspects - Dr Nadine Bohni, Head of Department, Kantonales Labor Zürich, Switzerland</h5>
<h5>09:45 - 10:15 | Latest developments - Prof Dr. Cristina Nerin, Dept of Analytical Chemistry &amp; Director of GUIA, University of Zaragoza, Spain</h5>
<h5>10:15 - 10:45 | Coffee Break</h5>
<h5>10:45 - 11:15 | Merck’s Input on E&amp;L/FCM - Dr Markus Obkircher, Director R&amp;D, Merck India, Bengaluru, India</h5>
<h5>11:15 - 11:45 | News from the Intertek Lab - Dr. J. Susanne Becker, Intertek AG, Switzerland</h5>
<h5>11:45 - 12:15 | Latest Applications in the field of E&amp;L - Dr. Rachel Sanig, Waters Corporation</h5>
<h5>12:15 - 12:30 | Panel discussion &amp; Closing - All</h5>
<h5>12.30 | End of the online workshop &amp; Lunch for attendees in Dättwil</h5>
<h4>Please note that:</h4>
<ul>
<li>The Workshop is free of charge and will be delivered in English
</li>
<li>Registration Deadline for in-person participation is April 30th 2024
</li>
<li>The number of seats is limited to 15 for the in-person participation at Waters AG (Dättwil)
</li>
</ul>
We are looking forward to welcoming you!
Jens Jacobsen, Waters Schweiz AG
Jürgen Marquardt, BASF Schweiz AG

Waters: 7th Swiss Workshop on Extractables and Leachables

7th Swiss Workshop on Extractables and Leachables

Join 7th Swiss Networking Workshop on migration of chemicals and potentially harmful substances from packaging materials.

As health-conscious consumers seek beverages that blend well-being and taste, functional beverages have emerged as a compelling choice. These specialized drinks are meticulously formulated to deliver targeted health benefits, often leveraging bioactive compounds like vitamins, antioxidants, and natural products. Moreover, the inclusion of natural sweeteners such as stevia not only enriches flavor but also augments nutritional value, making delicious and healthier products possible.
However, the dynamic landscape of the functional beverage sector presents a series of analytical challenges for manufacturers, ingredient suppliers, and testing laboratories. Ensuring product quality and regulatory compliance demands precise, reliable detection and quantification of key components such as vitamin B complexes and steviol glycosides. While traditional analytical methods have enabled market innovation and grow, modern liquid chromatography technologies can reduce time-to-result and increase measurement accuracy in complex samples. These analytical advancements can reduce cost, speed up product release, and strengthen consumer trust.
Join us for an insightful and engaging webinar to explore how Waters HPLC innovations can revolutionize your functional beverage analysis, ensuring your products stand out in a competitive market. Here’s what we’ll cover:
<ul>
<li>Vitamin B: We will navigate the complex landscape of B-group vitamins, addressing the potential adsorption issues due to their phosphate groups, which can adversely affect peak shapes and accuracy of chromatographic results. We understand the significance of precise product labeling, and we are dedicated to providing tools to help you meet in-house and regulatory standards.
</li>
<li>Steviol Glycosides: With guidance from the latest FAO/WHO JECFA Monograph 26 (2021), we are thrilled to present our improvement in the analysis of steviol glycosides. Our recent work has led to improved chromatographic resolution, paving the way for dependable quantification and adherence to regulatory benchmarks.
</li>
<li>MaxPeak High Performance Surfaces (HPS) Technology: Learn how our cutting-edge separation science technology helps to mitigate issues due to metal interactions and improve chromatography for various food applications.
</li>
</ul>
Whether you are developing new beverages or ensuring the quality of your current products, this session will provide you with the strategies and technologies to thrive in a dynamic and competitive market. Engage with our experts, participate in our live Q&amp;A, and take the first step towards overcoming your analytical challenges.
<blockquote>
Presenter: Jinchuan Yang, PhD (Principal Scientist, QA/QC, Waters Corporation)
</blockquote>
<blockquote>
Dr. Jinchuan Yang is a Principal Scientist Waters Corporation, and a subject matter expert in food and environmental applications, polymer chromatography, and LC and MS technologies. Since he joined Waters in 2002, his roles at Waters have mainly focused on application development, market development, and support for R&amp;D and training. He is an active participant in technical stakeholder groups at AOAC International and USP, serving as an expert panel member in AOAC SPIFAN and participating in review, recommendation and muti-lab testing of AOAC methods.
</blockquote>

Waters Corporation: Functional Beverages – Leveraging Analytical Innovations to Overcome Chromatographic Challenges

Functional Beverages – Leveraging Analytical Innovations to Overcome Chromatographic Challenges

Discover how Waters HPLC innovations can transform your functional beverage analysis, setting your products apart in a competitive market.

Functional Beverages – Leveraging Analytical Innovations 

<h4>PhenoAcademy Webinars Sample Preparation Series</h4>
Overview of Sample Preparation and Introduction to Filtration
<ul>
<li>Learn about Sample Preparation basics and why it is used</li>
<li>Understand how mechanisms for how samples are cleaned up for analysis</li>
<li>Discover how to select the correct filtration membrane, size and format</li>
</ul>
<blockquote>
Presenter: Laura Ward (Technical Specialist)
</blockquote>
<blockquote>
Laura Ward is a Technical Specialist for the Asia-Pacific region with Phenomenex, located in New Zealand. She completed an Honours thesis in the field of Geochemistry at the University of Sydney, Australia where she discovered a love for Analytical Chemistry. Following her academic pursuits, Laura joined Phenomenex Australia as a Technical Sales Consultant where she supported various industries and laboratories. After 5 years and a move to New Zealand, she has joined the Phenomenex Technical Team where she supports scientists globally with their method development, troubleshooting and application support. In her spare time Laura enjoys being outdoors in the New Zealand countryside and cooking from scratch, most recently venturing into cheesemaking!
</blockquote>
<blockquote>
Presenter: Lucia Geis Asteggiante (Technical Specialist)
</blockquote>
<blockquote>
Lucia is a Senior Technical Specialist that has recently joined Phenomenex’s Global Technical Team. She has a broad experience in the field of Analytical Chemistry with a particular focus on LC chromatography coupled with mass spectrometry. Lucia comes from a strong academic and regulatory background with experiences ranging from method development and analysis of small molecules to large protein complexes. She gained her Ph.D. at the University of Maryland - College Park, USA and did her post-doctoral work at the University of Oxford, UK. During her time as a researcher, she has authored/co-authored 23 scientific publications and 2 book chapters.
</blockquote>

Phenomenex: PhenoAcademy Webinars - Sample Preparation Series

Overview of Sample Preparation and Introduction to Filtration

Sample Preparation basics and why it is used.

Sample Preparation and Introduction to Filtration

<h4>Facing Challenges in Your Liquid Chromatography Workflow?</h4>
How do you tackle peak shape issues, pressure fluctuations, or retention time inconsistencies? Where do you turn for insights on method optimization or lab workflow management? If these challenges sound familiar, our upcoming session is your opportunity to find answers.
This exclusive session, led by a panel of seasoned Agilent LC experts, is designed to address the hurdles you face in your LC workflows. Get actionable insights, practical tips, and proven strategies to not only overcome these obstacles but to significantly enhance your workflow&#39;s efficiency. 
No challenge is too big or small for our discussion. Our goal is to give you the answers you need. 
When registering, you&#39;re invited to submit your burning questions and specific challenges, ensuring that our panel addresses what matters the most to you and provides tailored solutions. 
Why Attend?
<ul>
<li>Engage with Experts: Your chance to ask questions and get answers from leading Agilent LC experts</li>
<li>Exclusive Rewards: Receive a special promo code for LC Columns, a certificate of attendance, and an LC Workflow Resource Kit, filled with tools, guides, and application notes</li>
<li>Professional Growth: This session isn&#39;t just about solving today&#39;s problems; it&#39;s about equipping you for tomorrow&#39;s successes, reaffirming your commitment to staying at the forefront of analytical science</li>
</ul>
Don’t let workflow challenges slow you down.
<blockquote>
Presenter: Mr. Sunil B Lakhmapure (Technical Support Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Sunil holds 19 year plus experience in the analytical field with major exposure to LC/GC with Single Quad, Triple Quad and high resolution (QToF) mass spectroscopy. Started career in Pharma R&amp;D as a scientist with hands on experience on high end instruments like LC/MS, XRD &amp; NMR. Developed &amp; published application notes using Chromatographic techniques on real world samples/issues at various levels.
</blockquote>
<blockquote>
Presenter: Ms. Sreelakshmy Menon (Application Scientist, Agilent Technologies)
</blockquote>
<blockquote>
Sreelakshmy is working as an Application Specialist, at Agilent Technologies for the past 11 years. She has experience in working with Analytical techniques such as HPLC, UHPLC, CE, SFC, Method development solutions etc. Her primary interest is in developing liquid chromatography application solutions focusing on AQbD approach concentrating on pharma and biopharma workflows. To her credit she holds around12 publications on innovative Liquid Chromatographic solutions.
</blockquote>
<blockquote>
Presenter: Mr. Pintu Nandasana (Application Engineer, Solution Support Division, Agilent Techologies India)
</blockquote>
<blockquote>
Pintu Nandasana is working as Application Engineer at Agilent Technologies India from last 11 years. Education background, He has completed master’s in pharmacy. Currently as a Field System Specialist, he is supporting all PL29 products like LC, LCMS, GPC, 2D-LC, Preparative LC &amp; Method development solutions.
</blockquote>
<blockquote>
Presenter: Mr. Shreyas Tribhuvane (Business Development Manager, LC Columns, Agilent Technologies)
</blockquote>
<blockquote>
Shreyas has 10 years experience in working closely with biopharma customers in understanding their analytical characterization needs. As a BDM for Biocolumns and Glycan kits his prime responsibility is to work on the Biopharma market needs and communicate with Agilent solutions unit team in order to have apt solutions. He has a hands-on experience on Agilent Glycan sample prep kits and helped customers in setting up these kits for their glycan characterization work.
</blockquote>

Agilent: Decoding LC: Navigating Workflow Challenges

Decoding LC: Navigating Workflow Challenges

Insights, tips, and proven strategies to not only overcome obstacles but to significantly enhance your workflow's efficiency.

Explore the intricacies of the world through the lens of nucleic acids—the building blocks of life. Nucleic acids, including the DNA and RNA, play pivotal roles across diverse fields, such as the following examples:
<ul>
<li>Biopharmaceuticals: The development of mRNA vaccines for COVID-19 highlights the rapid advancements and powerful potential of nucleic acid therapeutics.</li>
<li>Food safety: Nucleic acid-based detection methods can act as an effective tool to identify foodborne viruses, detect food allergens, and even uncover instances of food fraud.</li>
<li>Forensics: DNA analysis can shed new insights into crime scene investigations.</li>
<li>Medical diagnostics: Nucleic acids can be used to detect genetic disorders, cancer biomarkers, and infectious diseases etc.</li>
</ul>
In this Shimadzu Digital Classrooms, join us as we learn more about nucleic acid analysis, using UV-Vis spectroscopy and microchip electrophoresis. We will be introducing Shimadzu’s innovative technologies —TMSPC-8i, BioSpec-nano, and MultiNA—and showcase how they each play a crucial role in various spheres of science and industry. Register now!
Learning Objectives:
<ul>
<li>Learn about the capabilities of Shimadzu&#39;s TMSPC-8i to measure thermal stability of DNA/RNAs</li>
<li>Learn about the convenience of using Shimadzu&#39;s BioSpec-nano for nucleic acid quantitation down to 1µL</li>
<li>Learn how Shimadzu&#39;s MultiNA system can automate gel electrophoresis with improved reproducibility and sensitivity</li>
</ul>
<blockquote>
Presenter: Tan Qi An (Application Specialist - Spectroscopy, Shimadzu - Asia Pacific Pte Ltd)
</blockquote>
<blockquote>
Qi An is a highly skilled professional with a bachelor&#39;s degree in Chemistry and Biological Chemistry from Nanyang Technological University, Singapore. His passion for food science led him to pursue a concurrent second major in Food Science and Technology from Wageningen University. With over two years of experience as an Application Specialist for spectroscopy and TOC instruments at Shimadzu Asia Pacific, Qi An has successfully conducted pre-sales demonstrations, sample analysis, and provided application support across diverse industries. His expertise and commitment to delivering high-quality solutions make him a valuable asset to the field of analytical chemistry.
</blockquote>
<blockquote>
Presenter: Naatasha Isahak, PhD (Market Specialist - Clinical and Forensics, Shimadzu Asia Pacific)
</blockquote>
<blockquote>
Naatasha Isahak is a Product Specialist for MALDI and Life Sciences Products from Shimadzu Asia Pacific Pte Ltd. She has received her PhD from the University of Sydney (Chemistry) working on surface initiated polymerisation on silica nanoparticles using RAFT polymerisation and bioorthogonal conjugation of peptides using Native Chemical Ligation. She then spent 5 years working on developing platforms for stem cells and organoids characterization that included working with microfluidic devices and helical polymers. Naatasha joined Shimadzu in 2022 to expand her experiences to MALDI characterization on materials and biological samples.
</blockquote>

Shimadzu: Discover the Building Blocks of Life – DNA and RNA Analysis Solutions

Discover the Building Blocks of Life – DNA and RNA Analysis Solutions

Nucleic acid analysis, using UV-Vis spectroscopy and microchip electrophoresis.

DNA and RNA Analysis Solutions

The metal-finishing industry faces the ongoing challenge of managing wastewater effectively, especially when treating dissolved metals such as copper and hexavalent chromium. These metals pose significant environmental risks and introduce compliance and financial challenges. Traditional ion exchange and chemical precipitation methods exhibit limitations in selectively targeting specific metals and rely on hazardous chemical handling and secondary waste product treatment. Both methods have limited effectiveness with increased metal concentrations, competing ions&#39; presence, and dissolved solids&#39; overall concentration.
Join James Landon, Chief Technology Officer at ElectraMet, as he introduces an innovative solution to conventional dissolved metal approaches, employing an electrochemical process to efficiently remove or convert dissolved metals. Beyond treatment, both sustainability and operational optimization hinge on real-time data. Learn how online Process Analyzer Technology (PAT) can revolutionize wastewater management, offering precise, real-time monitoring and analysis.
<h5>Key Learning Objectives:</h5>
<ul>
<li>Understand how the innovative electrochemical process overcomes challenges in metal finishing.</li>
<li>Learn how real-time monitoring and analysis can optimize the wastewater treatment process</li>
<li>Learn how integrating electrochemical processes with PAT can transform wastewater into revenue</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Process Engineers looking to optimize wastewater treatment processes or who are looking to implement PAT in their metal finishing lines</li>
<li>Sustainability and Operations Managers tasked with implementing eco-friendly practices within their organizations and overseeing the day-to-day operations of metal finishing lines</li>
<li>Environmental Compliance Officers tasked with ensuring their facilities meet regulatory standards for wastewater</li>
<li>Wastewater Treatment Consultants advising on wastewater management strategies</li>
</ul>
<blockquote>
Presenter: James Landon, PhD (Chief Technology Officer and Co-founder, ElectraMet)
</blockquote>
<blockquote>
Presenter: Brett Lane (Field Applications Specialist, Process Wet Chemistry, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Ann Thayer (Contributing Editor, C&amp;EN Media Group)
</blockquote>

Metrohm: Beyond Ion Exchange: Advancing Wastewater Treatment in Metal Finishing

Beyond Ion Exchange: Advancing Wastewater Treatment in Metal Finishing

Process Analyzer Technology (PAT) can revolutionize wastewater management, offering precise, real-time monitoring and analysis.

Beyond Ion Exchange: Advancing Wastewater Treatment

Recognizing the importance of cellular metabolism and the complexity of cell-cell heterogeneity, SpaceM has emerged as a promising technology with high potential for investigating single-cell metabolomics. SpaceM is expected to grant broad access to single-cell metabolomics, enabling investigations across diverse cellular models. Additionally, addressing the challenge of reproducibility, we present HT-SpaceM, a high-throughput method showcasing robust reproducibility in identifying metabolites in 10 cell lines, highlighting the promise of the SpaceM method in understanding cellular metabolism and disease.
<blockquote>
Presenter: Shawn Owens, Ph.D. (Imaging Mass Spectrometrist, SpaceM, BioInnovation Institute, Copenhagen, Denmark)
</blockquote>
<blockquote>
Shawn began his mass spectrometry imaging journey over 10 years ago, at the University of California- Santa Barbara- where he earned his PhD. With his degree in hand, he began a journey that led him through roles in industry- first at Bruker for nearly 5 years, followed by a year at BASF in New York. Eager to explore new horizons, Shawn embarked on a transformative journey in a startup, joining SpaceM in Copenhagen, Denmark- where he assumed the role of R&amp;D Team Leader and Mass Spectrometrist, a role he has held for the past 18 months.
</blockquote>

Bruker: High Throughput Single-Cell Metabolomics

High Throughput Single-Cell Metabolomics

We present HT-SpaceM, a high-throughput method showcasing robust reproducibility in identifying metabolites in 10 cell lines.

Protein ubiquitination is a versatile post-translational modification that orchestrates many cellular pathways. Ubiquitin ligases are key specificity factors in this system. The HECT-type ubiquitin ligase HACE1 controls membrane dynamics and redox homeostasis by mechanisms that are still poorly understood at a structural level.
We determined the cryo-EM structures of HACE1 alone and in complex with its physiological substrate, RAC1. Together with biophysical analyses including HDX-MS and mass photometry, our studies reveal how HACE1 is regulated and how it recognizes its substrate with specificity. I am going to provide an overview of this work with a specific focus on the use of mass photometry.
All participants in this webinar will receive a Certificate of Attendance.
<blockquote>
Presenter: Sonja Lorenz (Research Group Leader, Max Planck Institute for Multidisciplinary Sciences)
</blockquote>

Wiley: Mass photometry to study the oligomerization of HACE1

Mass photometry to study the oligomerization of HACE1

Studies reveal how HACE1 is regulated and how it recognizes its substrate with specificity.

<h5>In this webinar, you will:</h5>
<ul>
<li>Understand the distinct regulatory categorizations of mRNA therapeutics in the European Union and United States</li>
<li>Learn about the advantages of an EU-based mRNA drug substance manufacturer</li>
<li>Gain insights into regulatory requirements for LNPs in mRNA-LNP formulation</li>
</ul>
<h5>Detailed description:</h5>
In recent years, RNA-based therapeutics, particularly siRNA and mRNA-based drugs, have revolutionized the field of medicine. In this webinar, we will delve into the categorization of mRNA-based therapeutics in the European Union and the United States and examine the manufacturing requirements for mRNA drug substances in each region. Gain insights into the benefits of these regional differences for mRNA drug product development. Stay informed on the latest EU and US regulations impacting mRNA therapeutics, from drug substance to lipid nanoparticle (LNP) formulations. Stay ahead of the evolving regulatory landscape and discover opportunities for your mRNA drug development strategies.
An on-demand version of this webinar will be available after the live event using the same link. Register now and access the webinar at your convenience._
<blockquote>
Presenter: Dr. Oliver Daniel Schwich (Regulatory Expert - LSS Regulatory Management)
</blockquote>
<blockquote>
Oliver is a Regulatory Expert for mRNA drug substance offering located at the mRNA Center of Excellence in Hamburg, Germany. He is responsible for surveillance of mRNA-related guidance, assuring their phase appropriate application and supporting customers regarding drug substance CMC sections.
</blockquote>
<blockquote>
Before joining Merck KGaA, Darmstadt, Germany, Oliver worked as a RA Manager for a swiss generic pharmaceutical company. He holds a master’s degree in biotechnology from the Technical University Braunschweig, Germany and a Ph.D. in cellular RNA biology from the University Frankfurt, Germany.
</blockquote>
<blockquote>
Presenter: Melike Uzun (Regulatory Expert - LS Regulatory Management)
</blockquote>
<blockquote>
Mel is a Regulatory Expert within Pharma Registration at Merck KGaA, Darmstadt, Germany. Her responsibilities include CMC - particularly Module 3 Quality for established APIs, excipients, as well as manufacturing authority and GMP-certificate activities for mRNA-based drug substances with the respective competent authority.
</blockquote>
<blockquote>
Prior to joining Merck KGaA, Darmstadt, Germany, Mel gained relevant mRNA-based therapeutic, and vaccine application experience from industry including BioNTech, Mainz Germany, and Moderna, Cambridge, MA, USA. Mel holds a B.S. in Biotechnology from the University of Maryland, USA, and M.D.R.A from the University of Bonn, Germany.
</blockquote>

MiliporeSigma: Navigating the Regulatory Landscape of mRNA-based Therapeutics

Navigating the Regulatory Landscape of mRNA-based Therapeutics

Stay informed on the latest EU and US regulations impacting mRNA therapeutics, from drug substance to lipid nanoparticle (LNP) formulations.

Regulatory Landscape of mRNA-based Therapeutics

Advances in enzymatic digestion techniques combined with new software analysis tools that incorporate in silico digestion capabilities for mRNA sequences, demonstrate the potential for sequence mapping and confirmation of mRNA using high-resolution LCMS. These new advances have the potential to progress mRNA sequencing by LCMS into the same well-defined workflow currently in place for the peptide mapping of therapeutic proteins.
Dr. Ken Cook, senior European team leader for the biopharma/pharma expert support group at Thermo Fisher Scientific, and Keeley Murphy, software product manager at Thermo Fisher Scientifc, will discuss the use of an automated workflow which includes enzymatic digestion, high-resolution LCMS analysis, and data analysis for mRNA sequencing.
Key learning objectives
<ul>
<li>Discover a new automated workflow for mRNA sequencing by high-resolution LCMS</li>
<li>Learn how to implement routine sequence confirmation of mRNA using high-resolution LCMS</li>
<li>Explore new software analysis tools that incorporate in silico digestion capabilities for mRNA sequences</li>
</ul>
Who should attend?
<ul>
<li>Biopharmaceutical research scientists</li>
<li>Analytical scientists from biopharma companies working on or interested in mRNA based therapies</li>
<li>PhD students in any field of biotherapeutic drug development</li>
<li>Scientists looking to advance their knowledge of oligonucleotide and mRNA analysis</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Ken Cook (Senior European team leader for the biopharma/pharma expert support group, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Keeley Murphy (Software product manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Moderator: Moderator: Dora Wells (Editoral Team, SelectScience)
</blockquote>

SelectScience: Automated Workflow for mRNA Sequencing by High-Resolution LCMS

Automated Workflow for mRNA Sequencing by High-Resolution LCMS

We will discuss the use of an automated workflow which includes enzymatic digestion, high-resolution LCMS analysis, and data analysis for mRNA sequencing.