What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

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<h3>PFAS and us: The science</h3>
Driving change for a healthier world
Webinar series
<h4>Enrich your understanding of PFAS chemicals and their impact</h4>
Stay on the leading edge of all things PFAS with this enlightening monthly webinar series on testing workflows and cutting-edge technology that will demystify these “forever chemicals”.
Each month, in these 30-minute sessions, industry thought leaders unite to share the current state of PFAS testing and the far-reaching impact that PFAS have on human health and the environment. Build on your current knowledge of testing methodologies and research techniques that will help you develop your own innovative strategies for precise identification and quantitation. Make a lasting impact and join us for these conversations.
<h4>This Webinar:</h4>
July 17, 2024 10:00 a.m. PT/ 1:00 p.m. ET - Detecting and identifying known and novel per/polyfluoroalkyl substances (PFAS) in a marine apex predator
<blockquote>
Presenter: Carrie McDonough (Assistant Professor, Chemistry, Carnegie Mellon University)
</blockquote>
<h4>Other Webinars from the Series:</h4>
April 10, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3865">Examining the exogenous: PFAS and beyond</a>
<blockquote>
Presenter: Andrew Patterson (Vice President, Innovation and Development, Eurofins Environmental Testing America)
</blockquote>
May 8, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3866">Rapid PFAS testing with no compromises: direct-inject analysis</a>
<blockquote>
Presenter: Jamie Fox (PFAS Technical Program Manager, Enthalpy Analytical)
</blockquote>
June 12, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3867">Assessment of advanced analytical instruments to reach required limits of quantitation for PFAS in foods</a>
<blockquote>
Presenter: Susan Genualdi, PhD (Research Chemist, FDA, Center for Food Safety and Applied Nutrition, Office of Regulatory Science)
</blockquote>
August 14, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3869">Whole blood microsampling methods for PFAS exposure biomonitoring</a>
<blockquote>
Presenter: Andrew J. Morris, PhD (Professor, University of Arkansas for Medical Sciences, Research Career Scientist, Central Arkansas Veterans Affairs Healthcare System)
</blockquote>
September 11, 2024 10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3870">Analysis of ultra short-chain PFCAs to better understand environmental sources and fate</a>
<blockquote>
Presenter: Cora J. Young (Associate Professor, Department of Chemistry, York University)
</blockquote>

Detecting and identifying known and novel per/polyfluoroalkyl substances (PFAS) in a marine apex predator

Detecting and identifying PFAS in a marine apex predator

With the Paris 2024 Olympics on the horizon, governments, sport federations, and anti-doping agencies are preparing for athletes to be on the world stage. Delivering security, integrity, and athlete trust in an even playing field is of paramount importance. While anti-doping sample collection methods have long been resistant to change, new solutions are providing benefits for both athletes and organizations.
In this webinar, Gabe Baida of InnoVero will outline the current landscape of anti-doping testing today and the new innovations that are advancing the field.
Key learning objectives
<ul>
<li>Explore the state of anti-doping today</li>
<li>Reasons for adopting athlete-centric testing</li>
<li>Discover innovations in anti-doping technology</li>
</ul>
Who should attend?
<ul>
<li>Professionals interested in learning about the latest technologies and innovations in anti-doping</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Gabe Baida (Executive Director, InnoVero)
</blockquote>
<blockquote>
Moderator: Dora Wells (Content Creator, SelectScience)
</blockquote>

SelectScience: Athlete-Centric Innovations in Anti-Doping Testing

Athlete-Centric Innovations in Anti-Doping Testing

This webinar will outline the current landscape of anti-doping testing today and the new innovations that are advancing the field.

Ion chromatography is a versatile and widely applicable technique for ion analysis, making it indispensable in chemical manufacturing settings. By addressing unique analytical challenges, it plays a crucial role in ensuring robust process and quality control.
In this webinar, we’ll hear from David Miyamoto, lab technical manager at Kuehne Company and Kuehne Lab Services, on how he incorporated IC into his analytical workflow. David will discuss where to start with determining IC as the right tool, how it can replace more tedious methods, and what you’ll need to get started. He’ll provide insight into the thought process behind IC method development, while also discussing specific IC application examples from the chlor-alkali industry, including challenges he faced such as complex sample matrices, and how he overcame them.
Following David’s discussion, Linx Waclaski, IC Product Manager at Metrohm USA, will provide further background on the theory and inner workings of an ion chromatograph, including options for further streamlining your analyses.
Take part in our live Q&amp;A after the presentation to get your specific questions answered by our experts.
Key Learning Objectives:
<ul>
<li>Find out how IC can fit as an analytical tool within a chemical manufacturing environment</li>
<li>Learn about important considerations for developing an IC method and overcoming challenges</li>
<li>Understand the basics of IC from a theoretical and equipment standpoint</li>
</ul>
Who Should Attend:
<ul>
<li>Analysts, technicians, chemists who want to learn how IC can be incorporated into a chemical manufacturing setting</li>
<li>Quality lab managers considering implementing IC into their lab</li>
</ul>
<blockquote>
Presenter: David Miyamoto (Lab Technical Manager, Kuehne Company)
</blockquote>
<blockquote>
Presenter: Linx Waclaski (Product Manager for Ion Chromatography, Metrohm USA)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

Metrohm: Integrating Ion Chromatography in a Chemical Manufacturing Environment

Integrating Ion Chromatography in a Chemical Manufacturing Environment

Where to start with determining IC as the right tool, how it can replace more tedious methods, and what you’ll need to get started.

Integrating Ion Chromatography in a Chemical Manufacturing

Many chromatographers experience difficulty retaining and separating polar analytes. This will often require reanalysis using alternative methods or changing column chemistries. The pain can be minimized by understanding the mechanisms for polar analyte retention and using the proper column designed for these difficult separations.
<blockquote>
Presenter: Chris Collins, Ph.D. (Principal Product Marketing Manager, Waters Corporation)
</blockquote>
<blockquote>
Chris Collins, Ph.D. earned his Ph.D. in Analytical Chemistry from North Carolina State University in 2014. He has over 20 years experience in the pharmaceutical industry with a focus on LC/MS Method Development. Chris started working at Waters Corporation in 2018 as a Chemistry Account Manager for North Carolina/South Carolina. In August, he transitioned to the role of Principal Product Marketing Manager for ACQUITY UPLC columns, UHPLC columns, Guards, and MaxPeak Premier Column Technology.
</blockquote>

Waters Corporation: Going Beyond the Boundaries of Retaining and Separating Polar Compounds

Going Beyond the Boundaries of Retaining and Separating Polar Compounds

The pain can be minimized by understanding the mechanisms for polar analyte retention and using the proper column designed for these difficult separations.

Retaining and Separating Polar Compounds

SLIMS connects directly to the instruments, tracks samples and guides users through the defined workflows ensuring quality results. SLIMS also can track equipment, consumables and reagents verifying availability and fit for purpose.
SLIMS complements chromatography and mass spectrometry software by automatically importing results and generating reports for review before sharing them with customers. Key features in SLIMS like the ability to version workflows can help labs when new standards are published, while enabling compliance with quality systems and regulatory requirements that are key to achieving excellence in scientific outcomes.
<blockquote>
Presenter: Miles Bradshaw, PhD (Pre-Sales Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Miles specializes in helping customers leverage the latest technologies to manage their laboratories on the Agilent SLIMS team. Miles has a breadth of experience with asset performance management, compliance, analytical workflow automation, and leveraging data analytics to tackle hard problems within the cannabis industry. Prior to joining Agilent, Miles has worked in the cannabis lab industry developing molecular microbiological testing methods.
</blockquote>

Agilent: Digitize Your PFAS Workflows with Agilent SLIMS

Digitize Your PFAS Workflows with Agilent SLIMS

SLIMS complements chromatography and mass spectrometry software by automatically importing results and generating reports

Join us as we discuss the collaborative process that led from the introduction of Shimadzu’s Nexera UC analytical SFC systems to the Pharma-focused creation and launch of the Nexera UC Prep semi-preparative SFC systems.
We will explore the uses of SFC and see how the Shimadzu lineup has been shaped to meet the needs of Pharma SFC users through their direct input into instrument and software design and development.
We will host a Q&amp;A session after the presentation to address any questions or comments.
<blockquote>
Presenter: Brian Domanski (Strategic Collaboration Scientist, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Brian has over 10 years working to provide LC and SFC solutions for Pharma, as well as experience with a wide range of chromatography techniques including RP, NP, HILIC, IEX, SEC/GPC, and IC. His focus also includes scalable Informatics and Data Integrity solutions.
</blockquote>
<blockquote>
Presenter: Kevin Wons (Market Manager - Pharma/BioPharma, Shimadzu Scientific Instruments)
</blockquote>
<blockquote>
Kevin has recently joined Shimadzu as Market Manager for Pharma/BioPharma. He has spent the last 6 years at AstraZeneca in the Analytical Sciences Department for product and process characterization for biologics in the AstraZeneca pipeline. Kevin has applications experience with HPLC and LC-MS to do Tryptic Peptide Mapping, Intact Mass Analysis, Subunit Mass Analysis, and Oligosaccharide Profiling. For his last 3 years, he acted as the Glycan Subject Matter Expert (SME) while at AstraZeneca. His work experience also includes Maryland Psychiatric Research Center, where he prepared animal tissue samples such as Brain, Liver, Blood, and Placenta to measure kynurenine pathway metabolites on GC/MS and HPLC. He has a Master’s degree in Professional Studies (MPS) focused on Biotechnology which prepares science professionals to fill management and leadership roles.
</blockquote>

Shimadzu: Harnessing the Power of Collaboration: Shimadzu’s SFC Journey

Harnessing the Power of Collaboration: Shimadzu’s SFC Journey

The introduction of Shimadzu’s Nexera UC analytical SFC and the Pharma-focused Nexera UC Prep semi-preparative SFC systems

The Power of Collaboration: Shimadzu’s SFC Journey

USP general chapter &lt;129&gt; Analytical Procedures for Recombinant Therapeutic Monoclonal Antibodies is being revised to include a SE-UHPLC method in addition the existing SE-HPLC method. Inclusion of modern chromatographic tools allows for greater flexibility in development without the need for full method validation. Ms. Peckham will share updates to USP chapter &lt;129&gt; focusing on SEC. Dr. Liu will focus on SEC-MALS. They will explore characterizing differences between innovator and biosimilars, including forced degradation studies.
Key learning objectives:
<ul>
<li>Understand the recent revisions to USP&lt;129&gt; with a focus on size exclusion chromatography by HPLC and UPLC. </li>
<li>Implication of the revised guidance and the opportunities for method modernization </li>
<li>Understand complementary information gathered by adding Multi-Angle Light Scattering (MALS) detection coupled to your chromatographic system.</li>
</ul>
Who should attend:
Protein scientists involved with analytical LC method development
<blockquote>
Presenter: Niomi Peckham (Director, Pipeline Development, US Pharmacopeia)
</blockquote>
<blockquote>
Ms. Peckham is the Director of Pipeline Development in USP Global Biologics. The Pipeline Development Team works with scientific experts and stakeholders to develop physical and documentary standards used to support development and quality assessment of biopharmaceuticals. She holds a Master of Science in Molecular and Cellular Biology from SUNY Stony Brook. Ms. Peckham has spent most of her career at Pfizer and Alexion Pharmaceuticals, focusing on analytical development of protein-based biopharmaceuticals.
</blockquote>
<blockquote>
Presenter: Hongjiang &quot;Leo&quot; Liu, Ph.D. (Application Scientist, Waters Corporation)
</blockquote>
<blockquote>
Dr. Hongjiang &quot;Leo&quot; Liu is an Application Scientist in the Analytical Sciences at Waters Wyatt Technology in Santa Barbara USA. He has been mainly focused on developing protein-based applications with LC-MALSthat provide more in-depth information, are easier to adopt, and are more versatile. Before joining Waters Wyatt Technology, he was a startup biotech entrepreneur and an expert-in-house at the Hult Prize Foundation. He received his Ph.D. from the University of California, Los Angeles (UCLA), where he researched and built novel designer enzymes that can capture CO2 and store them as biofuels and biopolymers with renewable electricity.
</blockquote>
<blockquote>
Moderator: Moderated by: Suraj Dhungana, Ph.D., MBA (Director, Americas Pharmaceutical and Life Sciences Market, Waters Corporation)
</blockquote>
<blockquote>
Suraj is currently the Director of Americas Pharmaceutical and Life Sciences Market for Waters Corporation. In his prior roles, he managed Waters Global Biomedical Research market and Omics Market for the Americas. Suraj holds a Ph.D. in chemistry from Duke University along with an MBA from Poole College of Management at NC State University and has completed postdoctoral trainings at the Los Alamos National Laboratory and National Institute of Environmental Health Sciences (NIEHS). He has 20+ years of experience in the areas of biotechnology focusing on human health, drug development, biomarker discovery, and bio-therapeutics characterization. Current research interest includes characterization of protein bio-therapeutics/conjugates, Oligo nucleotides and mRNA, and deliver systems.
</blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

USP Chapter 129 Updates and SEC-MALS

USP general chapter <129> Analytical Procedures for Recombinant Therapeutic Monoclonal Antibodies is being revised.

<h4>Bruker LabCast Part I</h4>
<a href="https://lcms.labrulez.com/webinar/4126">Part II</a>
Learn about MALDI Imaging with neofleX™, the newest benchtop mass spectrometer from Bruker. From entry-level researchers to experienced mass spectrometry users, this instrument will help you accomplish your research goals. The continued innovation of neofleX™ brings together intuitive software, and modern hardware for seamless automation and use for all users. Depth of analysis and fast results are not compromised in this benchtop system.
During this webinar, you&#39;ll learn:
<ul>
<li>The multiomics capabilities of MALDI Imaging</li>
<li>Ease-of-use on a benchtop mass spectrometer</li>
<li>Analytical figures of merit of the neofleX™</li>
</ul>
Who should attend:
<ul>
<li>Researchers interested in expanding their spatial biology toolkit</li>
<li>Researchers who want translational research tools</li>
<li>Anyone who wants to expand their instrument knowledge</li>
<li>Researchers focused on maximizing multiomics capabilites</li>
</ul>
<blockquote>
Presenter: Joshua Fischer, Ph.D., (Imaging Applications Scientist, Bruker Scientific, Nashua, NH, USA)
</blockquote>
<blockquote>
Dr. Joshua Fischer is an Application Scientist at Bruker focusing on MALDI Imaging. Dr. Fischer completed his Ph.D. at Purdue University and is an expert on MALDI Imaging and MALDI-FT-ICR systems.
</blockquote>
<blockquote>
Presenter: Daniel Castro, Ph.D., (Imaging Application Scientist, Bruker Scientific, Billerica, MA, USA)
</blockquote>
<blockquote>
Dr. Daniel Castro is an Application Scientist at Bruker focusing on MALDI Imaging. Dr. Castro completed his Ph.D. at the University of Illinois-Urbana-Champaign, and is an expert in high-throughput MALDI measurements and MALDI Imaging.
</blockquote>
<blockquote>
Presenter: Kate Stumpo, Ph.D., (Senior Market Manager, Bruker Scientific, Billerica, MA, USA)
</blockquote>
<blockquote>
Dr. Kate Stumpo is a Market Manager which entails messaging and marketing for MALDI Imaging. Dr. Stumpo completed her Ph.D. at Texas A&amp;M University, and is an expert in MALDI applications, including MALDI Imaging.
</blockquote>

Bruker: A complete imaging solution with automated workflows using neofleX™

A complete imaging solution with automated workflows using neofleX™

Learn about MALDI Imaging with neofleX™, the newest benchtop mass spectrometer from Bruker.

Imaging solution with automated workflows using neofleX™

Are you facing persistent issues in your HPLC performance? Are you striving to optimize your HPLC performance and extend the life of your LC instruments?
Don&#39;t worry - Ask The Expert!
From column performance or instrument breakdowns to method reproducibility, we understand the challenges you encounter.
Join us for the second episode of our &quot;Ask the Expert&quot; series. Led by a panel of seasoned Agilent LC experts, this exclusive session is designed specifically to address and help you overcome most pressing maintenance and troubleshooting hurdles in HPLC operation.
No challenge is too big or small for our discussion, and our goal is to give you the answers you need. Get actionable insights, practical tips, and proven strategies to optimize your HPLC performance and ensure method robustness.
When you register, submit your burning questions ensuring that our panel addresses what matters most to you and provides tailored solutions. Attendees will also get access to a LC Resource Kit including troubleshooting guides, column selection guide, productivity maps, etc.
Don&#39;t let troubleshooting and maintenance challenges hinder your progress. Join us to get the most out of your HPLC system, enhance your technical skills, and take a significant step in your professional development.
Register now to secure your spot. Find expert solutions to your HPLC challenges and ensure the longevity and efficiency of your HPLC systems!
<blockquote>
Presenter: Mr. Sunil B Lakhmapure (Technical Support Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Sunil holds 19 year plus experience in the analytical field with major exposure to LC/GC with Single Quad, Triple Quad and high resolution (QToF) mass spectroscopy. Started career in Pharma R&amp;D as a scientist with hands on experience on high end instruments like LC/MS, XRD &amp; NMR. Developed &amp; published application notes using Chromatographic techniques on real world samples/issues at various levels.
</blockquote>
<blockquote>
Presenter: Mr. Nirmal Thakker (Application Engineer - LPSD, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Nirmal has done his PhD in quality assurance, pharmacy and masters in pharmacy from University of Pune. He has more than 15 years of overall industrial experience in working in Analytical Research &amp; Development for renowned multinational pharma companies like Glenmark, Torrent and Piramal. He has also worked with Spinco Biotech as an application specialist for LC. Presently, he is working with Agilent Technologies as a technical application support specialist for chromatography, dissolution and lab informatics. He has an expertise in chromatography, QbD, method transfer and mass spectrometry.
</blockquote>
<blockquote>
Presenter: Mr. Pintu Nandasana (Application Engineer, Solution Support Division, Agilent Techologies India)
</blockquote>
<blockquote>
Pintu Nandasana is working as Application Engineer at Agilent Technologies India from last 11 years. Education background, He has completed master’s in pharmacy. Currently as a Field System Specialist, he is supporting all PL29 products like LC, LCMS, GPC, 2D-LC, Preparative LC &amp; Method development solutions.
</blockquote>
<blockquote>
Presenter: Mr. Shreyas Tribhuvane (Business Development Manager, LC Columns, Agilent Technologies)
</blockquote>
<blockquote>
Shreyas has 10 years experience in working closely with biopharma customers in understanding their analytical characterization needs. As a BDM for Biocolumns and Glycan kits his prime responsibility is to work on the Biopharma market needs and communicate with Agilent solutions unit team in order to have apt solutions. He has a hands-on experience on Agilent Glycan sample prep kits and helped customers in setting up these kits for their glycan characterization work.
</blockquote>

Agilent: Decoding LC: Troubleshooting and Maintenance Challenges.

Decoding LC: Troubleshooting and Maintenance Challenges.

This exclusive session is designed specifically to address and help you overcome most pressing maintenance and troubleshooting hurdles in HPLC operation.

<h4>SCIEX OS software summer school</h4>
Master the essentials of SCIEX OS software with our live webinars this summer
Join us for our summer school series dedicated to enhancing your proficiency with SCIEX OS software. Over the course of next months, we offer a series of concise, 30-minute webinars designed to fit into your schedule.
Agenda:
<ul>
<li>23 July, 11:00 a.m. CET – Hardware configuration and instrument verification</li>
<li>6 Aug, 11:00 a.m. CET – Compound optimization</li>
<li>20 Aug, 11:00 a.m. CET – Data processing: quantitation</li>
<li>2 September, 11:00 a.m. CET – Data processing: screening</li>
</ul>

SCIEX OS software summer school - Hardware configuration and instrument verification

Join us for our summer school series dedicated to enhancing your proficiency with SCIEX OS software.