What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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<h4>LC-MS/MS fundamentals webinar series 2.0</h4>
Advanced techniques for data acquisition and processing
Elevate your analytical expertise with advanced techniques and workflows
Sharpen your data acquisition and processing skills with our LC-MS/MS 2.0 webinar series. Based on our LC-MS/MS 1.0 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows.
Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.
<h4>This webinar:</h4>
July 16, 2024 11:00 a.m. - 11:30 a.m. CET Automating data processing
Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.
<h4>Other webinars from the series:</h4>
May 7, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.cz/webinar/3820">Beyond the fundamentals</a>
May 21, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3852">Advanced MS/MS method building</a>
Gain insight into advanced features for maximizing sensitivity in your mass spectrometry analyses.
June 4, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3853">Mastering chromatography</a>
Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.
June 18, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3854">It&#39;s a TRAP! Getting the most out of your QTRAP system</a>
Learn when to utilize QTRAP functionality to create more selective and sensitive methods.
July 2, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3855">Troubleshooting at the source</a>
Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.
July 30, 2024 11:00 a.m. - 11:30 a.m. CET - <a href="https://lcms.labrulez.com/webinar/3857">Excel strategies for mass spectrometry data analysis</a>
Take an in-depth look at when and how to use Excel to increase your productivity.

SCIEX: LC-MS/MS fundamentals webinar series 2.0

Automating data processing

Elevate your analytical expertise with advanced techniques and workflows.

Automating data processing in LC-MS/MS

Proper instrument performance becomes increasingly important as demands for lab productivity rise. No one likes it when an LC system is down. Understanding the key components of an LC system, how it works, and the effects of certain instrument parts on chromatography are essential to keep LC systems operational.
In this webinar, we’ll provide an overview of the LC system, from solvent bottles to detector, with a focus on preventive maintenance and keeping systems running at optimal performance.
<blockquote>
Presenter: Mark Powell (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.
</blockquote>

Agilent Technologies: Care and Feeding of Your HPLC System: Preventive Maintenance Tips

Care and Feeding of Your HPLC System: Preventive Maintenance Tips

An overview of the LC system, from solvent bottles to detector, with a focus on preventive maintenance and keeping systems running at optimal performance.

HPLC System: Preventive Maintenance Tips

Join us for thought provoking webinar series on the entire value chain of therapeutic peptides.
Register now and get an opportunity to learn about:
<ul>
<li>Challenges in analytical test methods</li>
<li>Selection of analytical techniques and samples</li>
<li>Quality considerations for therapeutic peptide analysis</li>
<li>USP quality standards for synthetic peptides and its impurities</li>
<li>Sequence identification and confirmation</li>
<li>LC/MS based characterization workflow</li>
</ul>
<h5>Analytical Similarity Study in Generic Synthetic Peptide Drug Product: Navigating Standards and Techniques.</h5>
<ul>
<li>Dr. Rakesh Shekhawat</li>
<li>Dr. Manoj Kumar Metta</li>
</ul>
<h5>Peptide Characterization Using Agilent workflows.</h5>
<ul>
<li>Dr. Ashish Pargaonkar</li>
</ul>
<blockquote>
Presenter: Dr. Rakesh Shekhawat (Founder/Owner, Proantek Research Labs Pvt. Ltd)
</blockquote>
<blockquote>
Dr. Rakesh Shekhawat is a distinguished scientist with over 20 years of industrial research experience in the fields of synthetic peptides, biosimilars, and oligonucleotide characterization. Holding a Ph.D. in analytical chemistry, Dr. Shekhawat has led analytical research teams at prominent companies such as Intas and USV, contributed significantly to securing various regulatory approvals for biosimilars and synthetic peptides. Recently, he founded Proantek Research Labs Pvt. Ltd., a company dedicated to the advanced characterization of biosimilars, synthetic peptides, and oligonucleotides.
</blockquote>
<blockquote>
Presenter: Dr. Manoj Kumar Metta (Team Lead, Senior Scientist II Global Biologics, United States Pharmacopeia (USP))
</blockquote>
<blockquote>
Dr. Manoj Metta is a renowned Biological Scientist with 21 years of experience in Biologics Quality assessment, and Regulatory sciences. He has rich hands-on experience in commercial development of protein, and peptide biopharmaceutical products with emphasis on developing analytical control strategies, physicochemical and functional characterization, analytical method development/validations and CMC review to support product licensure in global markets.
</blockquote>
<blockquote>
Dr. Manoj brings a wealth of experience and expertise to his role at the US Pharmacopeia (USP), where he has served for over 12 years in various scientific capacities. His contributions span across multiple areas, including the development of Monographs, Reference standards and General chapters in therapeutic proteins, monoclonal antibodies, synthetic peptides, and oligonucleotides. Dr. Manoj is a frequent speaker at national and international biopharmaceutical and regulatory scientific events.
</blockquote>
<blockquote>
Academically, he received Ph.D. degree in Biotechnology from Gandhi Institute of Technology and Management, on “Development of novel mammalian expression systems by using polyomaviral elements in CHO cell line” for monoclonal antibody production”.
</blockquote>
<blockquote>
Presenter: Dr. Ashish Pargaonkar (Biopharma Workflow Commercialization Lead, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Ashish Pargaonkar is a Lead for Biopharma Workflow Commercialization team for India and also Manager for Centre of Excellences at Bangalore. A Microbiologist by education with a Ph.D, he holds more than 20 years of experience for Biopharma market applications on Hi Resolution Mass Spectrometry.
</blockquote>
<blockquote>
He has a long record of successful demonstrations and customer engagements on workflows for CQA’s, Intact mass, peptide mapping, HCP and Glycans using Agilent state-of-the art solutions. He has authored ASMS posters on media components and therapeutic peptides. Dr. Ashish has been invited speaker at several conferences and workshops at Biosimilar Congress, ADC 2018, IIT (Mumbai, Delhi), ICT (Mumbai), PSI events.
</blockquote>

Agilent: Advance Your Peptide Product Commercialization: Quality and Characterization Considerations

Advance Your Peptide Product Commercialization: Quality and Characterization Considerations

Join us for thought provoking webinar series on the entire value chain of therapeutic peptides.

Peptide Commercialization: Quality and Characterization

Immune cell activation, differentiation and function are metabolically demanding processes, and it has now become clear that nutrient access and metabolism play a major role in regulating lymphocyte fate and function. These axes of regulation are crucially important in shaping the immune system when it is activating in metabolically demanding environments, such as tumor tissue. Indeed, the immune response to cancer is characterized by several metabolic defects.
In this webinar, Dr. Delgoffe will discuss some of these metabolic insufficiencies within the T cell compartment of solid tumors and reveal novel insights into how these metabolic nodes may be modulated to improve the immune response to cancer.
Attend this webinar to learn:
<ul>
<li>How T cells are sensitive to metabolites</li>
<li>The metabolic programs enacted by T cells as they become exhausted</li>
<li>How immunometabolism may be leveraged for cancer therapy</li>
</ul>
<blockquote>
Presenter: Dr. Greg M. Delgoffe (Associate Professor of Immunology &amp; Director of the Tumor Microenvironment Center, University of Pittsburgh &amp; UPMC Hillman Cancer Center)
</blockquote>

Technology Networks: Immunometabolic Features of Cancer Immunity

Immunometabolic Features of Cancer Immunity

Discussion of metabolic insufficiencies within the T cell compartment of solid tumors and reveal how these metabolic nodes may be modulated.

<h4>Event Overview:</h4>
In this webinar, we present an mRNA characterization workflow that includes sample preparation, LC-MS methodology and bioinformatics processing for the evaluation of 5&#39; Cap efficiency, mapping of oligonucleotide sequence fragments, isomer resolution and Poly (A) tail intact mass analysis.
Who Should Attend:
<ul>
<li>Researchers and Scientists: Ideal for those seeking to expand their insights into LC-MS characterization techniques and applications of mRNA modifications.</li>
<li>Quality Control and In-Process Testing Professionals: Recommended for professionals involved in quality control, in-process testing, or analytical development.</li>
<li>Biopharmaceutical Industry Professionals: Valuable for those evaluating and optimizing their current mRNA-based therapeutics development and characterization solutions.</li>
</ul>
Key Learning Points:
<ul>
<li>Explore the LC-MS characterization method for 5&#39; Cap, coding sequence mapping, and Poly(A) tail analysis.</li>
<li>Gain insights into utilizing ion-pairing reversed-phase LC-MS to characterize mRNA drug substances.</li>
<li>Discover the benefits of utilizing BioTi core-shell column technology to improve sensitivity and resolution for oligonucleotide identification.</li>
<li>Review of bioinformatics processing workflow</li>
</ul>
<blockquote>
Presenter: Roxana Eggleston-Rangel (Senior Applications Scientist, Phenomenex)
</blockquote>

Phenomenex: mRNA Characterization - From 5’ Cap to Poly (A): What IPRP–MS Can Tell You

mRNA Characterization - From 5’ Cap to Poly (A): What IPRP–MS Can Tell You

Presentation of mRNA characterization workflow including sample preparation, LC-MS methodology and bioinformatics processing.

mRNA Characterization: What IPRP–MS Can Tell You

As the world seeks sustainable solutions, catalyst analysis has become pivotal in driving this transformation. Catalysts are indispensable across various industries, from energy to pharmaceuticals, due to their ability to optimize chemical reactions. Their importance is particularly evident in the advancement of green technologies such as artificial photosynthesis and biofuel production, both of which are critical in reducing our dependence on fossil fuels.
In our upcoming Digital Classrooms session, we will provide an in-depth guide to analyzing different types of catalysts. This includes those used in energy production, environmental applications, and beyond - demonstrating how they can pave the way towards a sustainable future.
Join us to explore the complexities of catalyst analysis and understand its impact on creating a sustainable future. Whether you are a researcher, an industry professional, or passionate about sustainability science, this session offers valuable knowledge and insights you wouldn’t want to miss.
Register now to secure your spot!
Learning Objectives:
<ul>
<li>Understand the role of catalyst analysis in promoting sustainability</li>
<li>Discover Shimadzu&#39;s innovative solutions in catalyst analysis</li>
<li>Gain insights from experts on real-world applications of these technologies</li>
</ul>
<blockquote>
Presenter: Jackie, PhD (Assistant Market Manager - Chemical &amp; Food, Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Jackie obtained his PhD in Analytical Chemistry from the National University of Singapore, He has garnered over 10 years of research, training, and facilitation experience. Jackie meets scientists from various industries to share technical knowledge and collect feedback, understanding their problems and helping them find well-suited solutions in GC-related analyses.
</blockquote>
<blockquote>
Presenter: Osamu Ishitani, PhD (Special Appointed Professor, Hiroshima University)
</blockquote>
<blockquote>
Prof. Dr. Osamu Ishitani is a Special Appointed Professor of Chemistry at Hiroshima University and an emeritus professor of Tokyo Institute of Technology. He received his B. Eng. from Kobe University (1982) and Dr. Eng. from Osaka University (1987). He spent three months as a visiting researcher at the Hahn-Meitner Institute (Berlin, Germany), then joined the National Institute for Resources and Environment (AIST) as a researcher, and moved to Saitama University as an associate professor in 1995. He moved to Tokyo Institute of Technology in 2002 and became a full professor in 2006. He is a Vice President of the Asian and Oceanian Photochemistry Association. Osamu Ishitani served as President of the Japanese Photochemical Association (2020-) and Vice President of the Chemical Society of Japan (2018-2020). He has been interested in artificial photosynthesis for longer than 30 years. The main scientific targets of his group are photocatalytic CO2 reduction, photochemical properties of transition metal complexes, photo-functional metal complexes, and hybrid photocatalysts consisting of metal complexes and semiconductor materials.
</blockquote>
<blockquote>
Presenter: Chanatip Samart, PhD (Associate Professor, Thammasat University)
</blockquote>
<blockquote>
Dr. Chanatip Samart is currently Associate Professor at Department of Chemistry, Faculty of Science and Technology, Thammasat University, Faculty of Science and Technology, Thammasat University. He received the B.Sc. in Industrial Chemistry from King Mongkut Institute of Technology Ladkrabang (KMITL). After that, he recieved the Master and Ph.D. in Chemical Engineering, from Kasetsart University. He was appointed lecturer in Department of Chemistry, Thammasat University in year 2006. He was deputy Dean, and Head of Chemistry Department in year 2009 and 2012, respectively. He received the award of outstanding young researcher from Thammasat University in year 2009. He has been awarded Talent mid-career fund by National Research Council of Thailand from year 2022-2024. His research interest is biomass conversion, catalysis and surface modification. His publication is more than 100 papers in ISI. He has been served as editorial board member of Discover Applied Science (Formerly S.N. Applied Science) Journal, Carbon Resources Conversion, and Journal of Metals Materials and Mineral.
</blockquote>

Shimadzu: Fuelling a Sustainable Future: Innovations in Catalyst Analysis

Fuelling a Sustainable Future: Innovations in Catalyst Analysis

An in-depth guide to analyzing different types of catalysts.

Innovations in Catalyst Analysis

Recent instrumental breakthroughs in mass spectrometry are a real game-changer and have opened new possibilities for proteomic analysis implying extreme sensitivity at high throughput.
On the one hand single cell proteome analysis has become feasible: we have pushed a timsTOF Ultra instrument to its limits to reach a proteome coverage of around 5.000 proteins identified in a single cell. On the other hand, chromatography and MS acquisition parameters optimizations combined with innovative AI-based data interpretation have enabled us to reach unprecedented coverage in immunopeptidomics with close to 20.000 immunopeptides identified from limited numbers of cells. We will show how fine application-specific optimizations of every step of the proteomics workflow are strongly beneficial to the output results.
<blockquote>
Presenter: Christine Carapito (CNRS Research Director, Co-head of the BioOrganic Mass Spectrometry Laboratory, CNRS and University of Strasbourg, France)
</blockquote>
<blockquote>
Presenter: Jeewan Babu Rijal (Post-doctoral fellow at the BioOrganic Mass Spectrometry Laboratory, CNRS and University of Strasbourg, France)
</blockquote>

Bruker: timsTOF Ultra: Immunopeptidomics and single cell proteomics explorations

timsTOF Ultra: Immunopeptidomics and single cell proteomics explorations

Fine application-specific optimizations of every step of the proteomics workflow are strongly beneficial to the output results.

timsTOF Ultra: Immunopeptidomics and proteomics

Here recent progress, challenges, and learnings from MS-based workflows at Symphogen will be present and illustrated through case-studies. A broad range of separation techniques are now routinely combined with mass spectrometry for in-depth biopharmaceutical characterization. Simultaneously, MS software tools have evolved to support storage, processing and reporting of the expanding MS data oceans.
Dr. Shane Bechler, Christof Mitterer, Maria Grübner, and Dr. Ken Cook from Thermo Fisher Scientific, and Dr. Dan Bach Kristensen from Symphogen, will discuss the recent progress, challenges, and learnings from MS-based workflows at Symphogen.
Additionally, they will present an overview of the technological highlights of a novel mono-disperse ion-exchange technology for both strong cation and anion exchanger. Applications for high-resolution charge profiling of biopharmaceutical modalities including charge variant analysis of monoclonal antibodies and recombinant proteins, and the determination of full and empty AAV viral vectors will also be explored.
Key learning objectives
<ul>
<li>Learn about separation techniques for in-depth biopharmaceutical characterization</li>
<li>Understand how MS software tools have evolved to support storage, processing and reporting of the expanding MS data</li>
<li>Explore novel mono-disperse ion-exchange technology for both strong cation and anion exchanger</li>
</ul>
Who should attend?
<ul>
<li>Lab managers and directors</li>
<li>Principal scientists</li>
<li>Laboratory analysts</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Shane Bechler (Senior R&amp;D Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Christof Mitterer (Global product manager for BioLC columns and consumables, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Dr. Dan Bach Kristensen (Principal scientist, Symphogen, a part of Servier)
</blockquote>
<blockquote>
Presenter: Maria Grübner (Senior Product Applications Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Dr. Ken Cook (EU manager biopharma expert support group, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Moderator: Carrie Haslam (Associate Editor, SelectScience)
</blockquote>

SelectScience: HPLC Reloaded: LC-MS Hyphenated Techniques for Biopharma

HPLC Reloaded: LC-MS Hyphenated Techniques for Biopharma

MS-based workflows and overview of a novel mono-disperse ion-exchange technology for strong cation and anion exchanger.

Event Program
<ul>
<li>Discover the fundamentals of multiparameter analysis and its role in increasing the productivity.</li>
<li>Explore real-world applications in pharmaceuticals, food &amp; beverage, and water testing.</li>
<li>Learn how automation in electrochemistry revolutionizes efficiency and empowers sustainable practices.</li>
<li>Gain insights into navigating compliance regulations seamlessly with METTLER TOLEDO&#39;s products and services.</li>
</ul>
In today&#39;s world, maximizing productivity is no longer optional. Across industries, there&#39;s a growing need for efficient and reliable methods to evaluate and optimize solutions. This is where multiparameter analysis emerges as a promising tool.
Precise pH measurement plays a crucial role in various sustainability initiatives. From ensuring optimal growth conditions in biofuel production to maintaining safe drinking water quality, pH data is essential. METTLER TOLEDO pH meters offer exceptional accuracy and reliability, making them an indispensable tool for reliable data in your sustainability journey.
The true strength of these meters also lies in their ability to seamlessly connect with other instruments for measuring critical parameters like refractive index, density, spectroscopic data etc. This enables analysis of a wider range of parameters simultaneously or in parallel. This comprehensive approach provides a holistic view of the samples, empowering one to optimize resource utilization, ensure product quality and safety, and develop sustainable solutions. By combining pH with other key parameters as mentioned above, deeper insights can also be obtained to minimize waste, maximize efficiency, and guarantee consistent product quality that adheres to safety regulations – all crucial aspects of sustainable practices.
This webinar, led by Dr. Reena Mascarenhas, Assistant Manager of Market Development, will explore multiparameter analysis and its role in streamlining the processes across various industries.
Join us for a Live Webinar on Multiparameter Analysis for Sustainable Solutions!
Register now and secure your spot!
For multiparameter analysis, what instruments can a pH meter be connected to?
Beyond parameters like conductivity, DO, ISE, and Redox, which can be measured by the same meter, a pH meter can be connected to other instruments like a refractometer, density meter, UV/VIS spectrometer, titrator, and balance.
In which industries can quality control labs leverage multiparameter analysis to improve their processes?
Multiparameter analysis empowers quality control labs across various industries. In the pharmaceutical sector, labs can ensure consistent drug quality by simultaneously measuring parameters like pH, conductivity, and concentration. The food and beverage industry, including the wine industry, benefits by monitoring key parameters like pH, conductivity, density, and dissolved oxygen to guarantee consistent taste, texture, and shelf life. It also plays a crucial role in monitoring safe drinking water (concentration of ions, pH, conductivity) and optimizing agricultural soil conditions (pH, conductivity, nutrient levels). By measuring multiple parameters simultaneously, labs achieve efficiency and ensure high-quality products across diverse industries.
<blockquote>
Presenter: Dr Reena Mascarenhas
</blockquote>
<blockquote>
Reena is an electrochemist, working for METTLER TOLEDO, with over five years of experience in training others in various aspects of the field. Her in-depth understanding, technical expertise, and engaging and informative training style make her a prominent speaker for this webinar.
</blockquote>

Mettler-Toledo: Multiparameter Analysis for the Lab: from Complexity to Simplicity

Multiparameter Analysis for the Lab: from Complexity to Simplicity

A Live Webinar for Optimizing Workflows for pH, Refractometry, and Density Determination.

Multiparameter Analysis for the Laboratories

After the approval of the final version of ICH Q14, dealing with the Analytical method development and the new update of the ICH Q2, analytical validation, incorporating the AQbD concept to the analytical testing, it’s becoming difficult to generate results at the same pace as we have done historically. However, these changes give the developers a powerful tool that assures the ruggedness and reliability of the analytical method.
In both webinars we will review, with our partner Chromsword, the chance to incorporate the new requirements proposed in the quality guidelines in the LC method development workflow, along with the automation possibilities. Easing the analytical and statistical effort in an unprecedent manner. 
During this webinar, the second in a two part series, we will share examples of use. Both from the real implementation in a pharmaceutical lab, and an example of complex development upon lipidic nanoparticles composition.
<blockquote>
Presenter: Dr. Aline Bayerle (Applikationsspezialistin für LC basierte Lösungen, Agilent Technologies)
</blockquote>
<blockquote>
Aline works as an application specialist for LC based solutions at Agilent. During 10+ years of working as an analytical chemist in both the pharmaceutical industry, a GLP/cGMP environment, and at Agilent, she was able to gain experience in a variety of applications.
</blockquote>

Agilent Technologies - Analytical Method Development under ICH Q14

Analytical Method Development under ICH Q14 Part 2

We will review the chance to incorporate the new requirements proposed in the quality guidelines in the LC method development workflow.