In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with Imad Haidar Ahmad, Scientific Research Director at Amgen leading separations for Discovery Chemistry and Externalization. Imad's career spans Novartis, Merck, and now Amgen, and his research on computer-assisted method development has landed multiple front covers in Analytical Chemistry.We dig into how his group builds retention models from as few as nine lab experiments to map an entire separation landscape — replacing weeks of trial and error with a resolution map you can query and rebuild from. Imad breaks down where this modeling approach struggles (large molecules, SFC, fully autonomous method development), walks through two-dimensional liquid chromatography in plain terms for anyone who's only worked in 1D, and shares the story behind his group's greenness-score mapping work — including where swapping acetonitrile for methanol worked, and where it didn't.We also talk career advice: what a typical day looks like leading a discovery-stage separations team, how undergrad and grad students can bridge the academia-to-industry gap, and what Imad sees as the field's biggest unsolved problem.<h3>Video Transcription</h3>Chromatographic method development is increasingly moving beyond trial and error toward workflows built on modeling, automation, multidimensional separations, and smarter use of data. In a recent interview, Haidar Ahmad discussed how these approaches are changing pharmaceutical analysis, why understanding the fundamentals of chromatography remains essential, and where some of the biggest opportunities in separation science still lie.Ahmad’s route into analytical chemistry was not entirely planned. As an undergraduate, he initially found inorganic and organic chemistry particularly attractive, but a final-year project focused on the analysis of additives in bread introduced him to the possibilities of analytical chemistry. During his PhD, his research moved into polymer analysis and structure–property relationships. His interest in chromatography deepened when he encountered two-dimensional liquid chromatography (2D-LC), particularly its ability to reveal forms of polymer heterogeneity that could not be adequately characterized using a single separation technique. This eventually led to postdoctoral work with Peter Carr, where Ahmad focused on chromatographic separations and the fundamentals of chromatography.His subsequent career in the pharmaceutical industry exposed him to different stages of drug development, from late-stage method development and validation to drug substance characterization, purification, and finally discovery-stage separations. That breadth of experience has shaped his view of chromatography as both a scientific discipline and a practical problem-solving tool. In late-stage development, the objective may be a highly robust method capable of successful validation and transfer between laboratories. In discovery, by contrast, speed becomes critical because teams may be working with thousands of molecules while trying to identify the best candidates to advance.&nbsp;<h4>Discovery-stage chromatography: speed, prioritization, and throughput</h4>For a separations team supporting drug discovery, the working day is shaped by a constant flow of requests from medicinal chemistry and other partner groups. The challenge is not simply to perform the analyses, but to decide which requests are most urgent, remove bottlenecks, communicate with project teams, and continuously improve existing workflows.According to Ahmad, one of the most important questions is whether a task can be done faster or in a more high-throughput way. Discovery laboratories operate under strong time pressure, and efficiency therefore becomes part of the scientific strategy rather than merely an operational concern. As he describes it, the work combines science, prioritization, leadership, and communication, all with the aim of moving the most important projects forward as quickly as possible.<h4>In silico modeling: letting the computer perform the experiments</h4>One of the central themes of Ahmad’s work is in silico chromatographic modeling. In practical terms, the approach begins with a carefully designed set of laboratory experiments—often around nine runs. The resulting data are fed into software that builds retention models describing how analyte retention changes with variables such as mobile-phase composition and temperature.The software can then generate a resolution map representing a much larger separation space than could realistically be tested experimentally. Instead of running dozens or hundreds of conditions by trial and error, the analyst can identify regions where critical peak pairs are predicted to achieve the best separation.For Ahmad, the value of these maps goes beyond simply finding the condition with the highest resolution. Each point on the map represents a chromatographic condition, while the visual output shows where poor and strong separations are expected. A broad region of good resolution is particularly useful because it points toward a more robust operating space rather than a narrowly defined optimum that might be sensitive to small variations in experimental conditions.He therefore prefers to think of a chromatographic model as a map or database, rather than simply an optimization tool. If an impurity later disappears from the process, the corresponding peak can be removed from the model and new conditions selected without repeating the complete experimental study. Conversely, if a new degradation product appears during stability studies, the peak can be added and the existing model used to search for updated separation conditions.<h4>Why trial and error still has a place</h4>Modeling does not mean that conventional trial-and-error method development has disappeared. Ahmad points out that it remains useful in early discovery, particularly for fit-for-purpose methods where speed is more important than achieving a fully optimized separation.The situation changes later in development. A method intended for validation, transfer, or long-term routine use carries much greater risk. Trial and error may identify a condition that appears satisfactory without revealing whether it represents the most robust region of the separation space. If that method later fails during transfer or routine operation, the consequences can be costly.For late-stage methods, the additional investment required to build a model can therefore reduce risk over the much longer lifetime of the analytical procedure.<h4>Models are only as good as the experiments behind them</h4>Chromatographic modeling is not automatic proof against poor method development. Ahmad compares the technology to a GPS: it is highly useful, but only if the analyst understands where they are trying to go.Careful experimental design remains essential. Incorrect dwell-volume or column-volume information, as well as insufficient column re-equilibration, can produce misleading predictions. Importantly, these problems may not be obvious when the experimental runs used to construct the model are compared with their calculated values. They may emerge only when the software begins predicting new conditions within the modeled design space.Modeling is also easier for some separations than others. Ahmad describes reversed-phase LC of small molecules as comparatively straightforward, while large biomolecules are more challenging because their retention can be highly sensitive to small changes in mobile-phase composition and temperature.Other separation modes present additional difficulties. Ahmad notes that his teams have found supercritical fluid chromatography (SFC) particularly challenging to model successfully. Fully autonomous method development remains another unresolved goal: current tools can automate parts of optimization, but analyst input is still required.<h4>Why two dimensions can reveal what one dimension cannot</h4>Ahmad’s early interest in 2D-LC came from polymer characterization. He explains the principle simply: one-dimensional chromatography may answer one question about a sample, while two complementary dimensions can answer two.Two copolymers, for example, may have similar molecular size and therefore coelute in size-exclusion chromatography. A second separation mechanism based on interaction with a stationary phase may distinguish them according to chemical composition. Combining both dimensions provides a more complete description of complex distributions involving molecular weight, composition, sequence, and other forms of heterogeneity.Historically, the complexity of both instrumentation and software limited wider adoption of 2D-LC. Ahmad recalls systems requiring several computers and multiple software packages. That situation has improved substantially, and 2D-LC is now easier to implement across research and pharmaceutical development.Nevertheless, modeling multidimensional separations still presents challenges. While models can be built for individual fractions or dimensions, current software does not easily identify a common optimum across multiple models simultaneously. Much of this comparison still requires manual work.<h4>Bringing sustainability into method development</h4>Another area where Ahmad sees room for change is green analytical chemistry.Traditional pharmaceutical method development has understandably prioritized resolution, sensitivity, robustness, and turnaround time. Sustainability has often been treated as a secondary consideration, particularly at the analytical scale, where solvent use appears small compared with preparative chromatography or manufacturing.That mindset is beginning to change. Ahmad argues that greenness metrics should increasingly be incorporated directly into method-development software. If analysts could immediately see how changes in flow rate, gradient conditions, solvent choice, or other parameters affect the environmental profile of a method, sustainability could become part of routine optimization rather than an assessment performed after the method has already been developed.The objective, however, is not to pursue the greenest possible method regardless of analytical performance. Ahmad emphasizes the need to develop methods that are as green as possible while remaining fit for purpose. Modeling can potentially help balance these competing requirements by identifying conditions that preserve separation performance while allowing greener solvents or alternative chromatographic conditions to be introduced.<h4>Advice for young chromatographers: do not treat HPLC as a black box</h4>For students learning chromatography, Ahmad’s central recommendation is straightforward: do not treat the HPLC system as a black box.Rather than collecting chromatograms by making random changes, he encourages analysts to build a mental model of the separation. When temperature, gradient, or another parameter is changed, they should ask why retention or selectivity changed and use each experiment to improve their understanding of the system.He also recommends making use of educational resources on chromatographic fundamentals and method development and, importantly, not being afraid to experiment with instrument conditions. Modern systems are robust, but the analyst still needs to understand the underlying separation science in order to use them effectively.The same proactive attitude applies to career development. Ahmad strongly encourages students to seek industry internships and make connections beyond their own institutions. Conferences and poster sessions can provide natural opportunities to meet scientists from industry, discuss research, and ask about internship opportunities. Based on his own experience working with undergraduate and graduate interns, these collaborations can benefit both students and research teams and may strongly influence later academic or career decisions.<h4>What still needs to be solved?</h4>Asked about the biggest unresolved challenges in separation science, Ahmad does not begin with technology. Instead, he points to a shortage of well-trained analytical chemists with a strong understanding of chromatographic fundamentals.Alongside education, he identifies several technical opportunities. One is truly autonomous method development, where an instrument and software could optimize a separation without continuous analyst input. Another is the ability to predict chromatographic selectivity directly from molecular structure.Such capability could be especially valuable in high-throughput discovery environments. If a laboratory could predict which stationary phase and conditions were most likely to separate a particular set of compounds or isomers before entering the laboratory, method development could become considerably faster and more efficient.Taken together, Ahmad’s perspective points toward a future in which chromatography becomes increasingly model-driven, automated, multidimensional, and sustainability-aware. But the technology does not eliminate the need for chromatographic knowledge. On the contrary, as analytical tools become more powerful, understanding the fundamentals may become even more important—the software can explore the separation space, but the scientist still needs to know what question to ask and how to interpret the answer.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 In Silico Modeling, 2D-LC & Green Chromatography: Imad Ahmad on the Future of Method Development

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

Researchers from CATRIN at Palacký University, together with colleagues from VSB – Technical University of Ostrava and Charles University, have developed a new graphene ink for producing inexpensive and sensitive printed sensors. Using single-atom engineering, they incorporated copper atoms into the material, enabling highly sensitive detection of dopamine – a neurotransmitter responsible for transmitting signals between nerve cells. The approach, <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">presented in the journal Advanced Science</a>, has strong potential for use in medical diagnostics.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp 117w," sizes="100vw" width="1920" height="2560">Single-atom engineering makes it possible to control the chemical reactivity of materials at the level of individual atoms. Its broader application in medical diagnostics has so far been limited by a lack of atomically engineered materials and printable inks based on them. Researchers have now developed a new class of single-atom-doped graphene materials as well as an ink for producing sensitive printed sensors. Copper proved particularly effective for dopamine detection, which is important for biomedical research and the development of advanced diagnostic technologies for diseases including Alzheimer’s disease.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp 117w," sizes="100vw" width="1920" height="2560">“The NGA-Cu ink we developed enables the production of fully inkjet-printed, sustainable paper-based electrodes at very low cost. Each sensor requires only 1.3 micrograms of active ink, costs approximately one Czech koruna and provided a stable response for at least three months. The ink is also fully compatible with commonly available materials inkjet printers. This creates significant potential for producing affordable, digitally designed sensors whose function can be tailored atom by atom,” said&nbsp;the corresponding author of the <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">paper</a> Michal Otyepka.The basis for this new generation of single-atom inks was nitrogen-doped graphene acid (NGA), which CATRIN researchers developed several years ago. They have now progressively enriched it with different individual metal atoms, including copper, iron and manganese.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp 117w," sizes="100vw" width="1920" height="2560">“We found that the electrochemical response depends fundamentally on the type of atom used. While copper atoms significantly enhance the dopamine signal, other metals suppress it. What matters, therefore, is not only the presence of isolated metal atoms but also the specific metal involved. This is a very important finding for the further development of biosensors,” explained first author Martin-Alex Nalepa.The published results introduce a new class of sensing materials with considerable potential for medical diagnostics. The researchers present a broadly applicable platform for developing graphene inks containing individual metal atoms and open the way towards scalable, reproducible and material-efficient production of advanced electrochemical sensors.&nbsp;“We are only at the beginning and are fascinated by the new possibilities opening up before us. We can see that by changing the type of atom, we can control the sensitivity and selectivity of the sensor. In addition to dopamine, we can also determine immunoglobulins very precisely – antibodies whose levels and types may serve as important indicators of autoimmune or infectious diseases,” emphasised Radek Zbořil, a co-author of the paper.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp 117w," sizes="100vw" width="1920" height="2560">Electrochemical biosensors can find applications not only in medicine but also in food quality control, drinking-water monitoring and environmental pollution monitoring. Their key components are functionalised electrodes, and graphene-based materials have proved particularly suitable for their production. A key factor in the success of the Olomouc researchers was the targeted preparation of graphene ink with the required properties.The research was supported by the TECHSCALE project under the Johannes Amos Comenius Programme.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics: Project TECHSCALE (No. CZ.02.01.01/00/22_008/0004587) is financed by the MEYS OP JAC Excellent research programme, supported by ERDF/ESF." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg 245w," sizes="100vw" width="620" height="87"><blockquote><h4>Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">Single-Atom-Enhanced Fully Inkjet-Printed Electrochemical Sensor for Dopamine Detection</a></h4>Martin-Alex Nalepa, David Panáček, Vítězslav Hrubý, Matěj Jendřišák, Rostislav Langer, Michal Langer, Petr Jakubec, Ivan Dědek, Vojtěch Kupka, Michal Mazur, Radek Zbořil, Michal OtyepkaAdv. Sci., 2026, e76796<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/advs.76796">https://doi.org/10.1002/advs.76796</a><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Single-atom (SA) engineering offers atomic-level control over interfacial reactivity, yet the lack of printable SA-based inks hinders its practical translation into electrochemical sensing. Here, a fully water-based inkjet-printable ink is introduced based on nitrogen-doped graphene acid (NGA) hosting atomically dispersed Cu centers (NGA-Cu-ink). The ink enables digitally controlled, spatially defined deposition and fabrication of low-cost ($0.04 per sensor), fully inkjet-printed sustainable electrodes on paper. A comparison of NGA functionalized with different SA dopants (Cu, Mn, Fe, Ce) identifies a strong dopant-dependent electrochemical response, with Cu uniquely enhancing the analyte signal while other dopants suppress it, demonstrating that SA identity is a decisive design parameter in printed sensing interfaces profiling sensitivity and selectivity. The functional role of the NGA support is to provide dense anchoring sites (nitrogen and carboxylate groups) that stabilize atomically dispersed metal centers and create adsorption- and electron-transfer–active microenvironments. The NGA-Cu-ink yields enhanced dopamine oxidation, enabling quantitative detection on fully printed devices (limit of detection 9.7 µM; linear range 50–400 µM) and printed-on-electrode platforms (10.6 µM), while maintaining &lt;10% signal variation over 11 weeks. The approach establishes a general route to single-atom graphene-based inks for scalable, reproducible, and low-material-consumption manufacturing of advanced electrochemical sensors.

Atom by atom. Scientists reveal a new direction in medical diagnostics

Article

Academy

Scientists

Interview

Science and research

Video

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Fisher Scientific

Shimadzu Corporation

SCIEX

Waters Corporation

<h5>Session 1: Milk adulteration</h5>
There are so many food frauds that are a threat to consumers. Learn about a complete GC based solution on how to detect adulteration in milk. We will also cover workflows using ion chromatography to profile milk glycans, lactose and carbohydrates to ensure quality products.
<h5>Session 2: Mineral oil saturated hydrocarbons and aromatic hydrocarbons (MOSH/MOAH)</h5>
A 10 year old topic that is still the focus of chemists all over the world. Here, a fully automated solution will be presented, based on the LC/GC technique that is widely used in this field.
<blockquote>
Presenter: Daniela Cavagnino (Product Marketing Manager, GC and Autosampler and Applied Technologies, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Daniela Cavagnino received the master’s degree in chemistry at the University of Milan, Italy. She started her career in gas chromatography at Thermo Fisher Scientific spending several years in the R&amp;D laboratories working on GC technology innovation. Then, she conveyed her technical background into product management and marketing management roles with more than 10 years’ experience in promoting GC/GC-MS technology and applications in several different market segments.
</blockquote>
<blockquote>
Presenter: Jorn Hofsteenge (Analytical Support Specialist, Interscience BV, Netherlands)
</blockquote>
<blockquote>
Jorn is currently working withInterscience BV, Netherlands as an analytical specialist. He is a graduate in chemistry with specialization in forensic chemistry. He has around 10 years of experience and possesses a wide range of knowledge and skills in the fields of analytical chemistry and organic chemistry.
</blockquote>
<blockquote>
Presenter: (Wai-Chi Man, Product Marketing Manager, IC/SP, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Recently moved into Product Marketing from Application Sales Support role within ThermoFisher Scientific but was a customer.
</blockquote>
<blockquote>
Specialised in Ion Chromatography (IC) for over 32 years with 35 years in Analytical Chemistry experience in a wide range of techniques (IC, LC, GC, CE, MS, NMR, ICP, FTIR etc). Spent 7 Years at Wellcome Research supporting Structural Elucidation for Medicinal Chemists using a wide range of analytical instrumentation. A total of 20 Years in the Pharma industry as part of GlaxoSmith Kline, from Research to transferring methods to Manufacturing. Worked on and responsible for many drugs from anti-viral to respiratory.
</blockquote>
<blockquote>
Have been working for ThermoFisher Scientific since 2011 working with a wide range of customers from varies industry, providing ion chromatography support in method development, troubleshooting, sample preparation, trainings, seminars, events and workshops. Within this time, I have built a greater knowledge, skill and understanding within varies areas in IC such as IC-MS, IC-ICP-MS, HPAE-PAD analysis, metabolomics and polar pesticides.
</blockquote>
<blockquote>
I am passionate in promoting STEM (Science Technology Engineering Mathematics) to future Scientists by encouraging, nurturing and supporting them.
</blockquote>

Thermo Fisher Scientific: Emerging Topics in Food Safety

Food integrity and QC

Webinar will cover topics like Milk adulteration or Mineral oil saturated hydrocarbons and aromatic hydrocarbons (MOSH/MOAH) analysis.

<h5>Session 1: Beer off flavours</h5>
What would you do if suddenly the taste of your beer was different? This is a big issue for manufacturers and where GC-MS analysis can help. 
<h5>Session 2: Beer Integrity and quality</h5>
Beer, malt and wort testing is essential to producing a quality product, off flavours are the way to turn your customers off your products. Many of the tests performed on beer samples can require multiple analyzers, but there is another way to analyze multiple parameters at the same time.
The Thermo Scientific Gallery Plus Beermaster discrete analyzer can deliver reliable analyses for multiple tests such as bitterness, acidity and sugar content—all from a single sample and run up to 350 tests per hour. This system’s automated workflow and fast analysis work together to ensure high throughput and low cost-per-test. Because the instrument operates without manual intervention, your staff can enjoy walkaway productivity and focus on more strategic tasks in the lab. More testing, from fewer instruments and with fewer techs.
This webinar will show how you can work smarter and ensure the quality of your product.
<h3>Who should attend:</h3>
<ul>
<li>Lab managers and analysts that have analytical challenges when testing food and beverages</li>
<li>QA/QC and research laboratories that want to keep up to date and also be ready for future challenges</li>
<li>Lab leaders who want to leverage the full analytical potential of their analytical instrumentation</li>
<li>Scientists/chemists/analysts interested in using isotope fingerprints for food analysis</li>
</ul>
<blockquote>
Presenter: Spencer Britton (Senior Quality Lab Specialist, Creature Comforts Brewing Company, Athens)
</blockquote>
<blockquote>
Spencer Britton is a Senior Quality Lab Specialist at Creature Comforts Brewing Company in Athens, GA. Before joining the brewing industry in May 2017, he worked in a biopharmaceutical laboratory focusing on protein chemistry and ELISA development. Spencer is passionate about ensuring quality products and practices, which has led him to collaborate across the brewing industry to help establish key testing protocols.
</blockquote>

Food integrity and QC - Maintaining quality in the brewing process

This webinar will show how you can work smarter and ensure the quality of your product. Topics like Beer off flavours or Beer Integrity and quality.

Food integrity - Maintaining quality in the brewing process

<h5>Session 3: Addressing authenticity of fish oils by online coupling of GC-IRMS with an organic mass spectrometer.</h5>
As fish oils become a popular and precious source of omega-3 fatty acids, the risk of mislabeling and adulteration has risen significantly. The fatty acid profiles of different fish oils do not often allow the discrimination between different sources and geographical origins. In this study, the compound specific multi-isotope analysis of fatty acids allowed the discrimination of fish oils from different provenance, following risk-based comparisons from market experience. In the light of emerging cases of food fraud, we present how GC-MS-IRMS advanced technology can tackle these problems for addressing authenticity of fish oils.
<h5>Session 4: Technical Spotlight: Sugars in Honey by HPLC - discover how to analyse this popular sweet treat, and, is it all that you expect?</h5>
<h3>Who should attend:</h3>
<ul>
<li>Lab managers and analysts that have analytical challenges when testing food and beverages</li>
<li>QA/QC and research laboratories that want to keep up to date and also be ready for future challenges</li>
<li>Lab leaders who want to leverage the full analytical potential of their analytical instrumentation</li>
<li>Scientists/chemists/analysts interested in using isotope fingerprints for food analysis</li>
</ul>
<blockquote>
Presenter: Dr. Mario Tuthorn (Senior Product Marketing Specialist, Thermo Fisher Scientific)
</blockquote>
<blockquote>
After completing his PhD in Isotope Biogeochemistry at the University of Bayreuth, Germany, Mario joined Thermo Fisher Scientific in Bremen in 2016 as an application specialist for the Isotope Ratio Mass Spectrometry portfolio, developing applications and methods for compound specific isotope analysis in a variety of fields by using gas and liquid chromatography. Since 2019 Mario has taken on Product Marketing role for gas isotope ratio MS and organic elemental analyzer instrumentation, focusing on product positioning and marketing activities.
</blockquote>
<blockquote>
Presenter: Dr David Psomiadis (Chief Business Officer, Imprint Analytics GmbH)
</blockquote>
<blockquote>
David Psomiadis (PhD) works as Chief Business Office at Imprint Analytics GmbH. Since 2013, he has settled in Neutal, Austria, as a member of the grounding team of Imprint Analytics. He has published more than 70 contributions in scientific journals and conferences, and he holds a regular member position in international committees and working groups (eg. AOAC, CEN) related to food authenticity testing. In his role at Imprint Analytics, he leads the global Food Authenticity business strategy, brand development and collaborations within global networks and international consortia.
</blockquote>

Food integrity and authenticity

Addressing authenticity of fish oils by online coupling of GC-IRMS with an organic mass spectrometer or Sugars in Honey by HPLC.

<h3>Key Learning Points:</h3>
<ul>
<li>Understanding chromatographic Interactions
</li>
<li>Identifying Sytem/method/column related issues
</li>
<li>Choosing right mobile phase/column
</li>
<li>Proper column washing and storage conditions
</li>
</ul>
<blockquote>
Presenter: Dr. Rajesh Babu Dandamudi (Technical Manager, Phenomenex)
</blockquote>
<blockquote>
Rajesh Babu has more than 11 years of chromatography experience and is an expert in practical HPLC/UHPLC/LC-MS/GC-MS method development and troubleshooting. Before joining Phenomenex, Dr. Rajesh worked as a Faculty member at Sri Sathya Sai Institute of Higher Learning, Deemed University. He was instrumental in establishing a state-of-the-art mass-spec core facility consisting of GC, HPLC, GC-MS, LC-MS/MS, and HRMS systems. His research work was in the area of clinical metabolomics. 
</blockquote>

Phenomenex: 5 Common LC Troubleshooting Mistakes and What to Do Instead

5 Common LC Troubleshooting Mistakes and What to Do Instead

Chromatographic Interactions. Sytem/method/column related issues. Right mobile phase/column. Proper column washing and storage conditions.

LC/MS is now widely used for many clinical and forensic toxicology applications due to the inherent specificity and sensitivity of the technology. Sample preparation and pre-treatment requirements of LC/MS can still be a bottle neck in high volume testing laboratories resulting in time consuming and labor intensive manual steps.
This presentation will describe how automation of LC/MS methods can improve productivity and analytical results.
Explore the journey of the sample through an automated LC/MS workflow from sample receipt to the reporting of results back into a LIMS. The advantages of automation are discussed, along with automation options for the toxicology LC/MS workflow based on different laboratory needs. Sample tracking is a key part of any workflow and has been considered in all automated options shown, including other important requirements to ease the extraction and analysis process for the analyst.
<h5>Learning Objectives - What you will learn:</h5>
<ul>
<li>Why Automation is important for LC/MS workflows in toxicology applications
</li>
<li>What steps can be automated in LC/MS workflows
</li>
<li>How automation can improve analytical performance and productivity
</li>
</ul>
<blockquote>
Presenter: Robert Wardle (Senior Scientist, Clinical Applications, Waters Corporation, UK)
</blockquote>
<blockquote>
Rob Wardle is a currently a Senior Scientist employed at Waters Corporation in Wilmslow, UK, performing research and development of applications and products for Clinical and toxicology applications. Rob has wide experience of method development in clinical and toxicology applications and in recent years has played a key role on developing offline and online automation methods and protocols. Rob joined Waters in 2010 as a Clinical Validation Scientist, playing a key role in verification activities for LC/MS reagent kits. Rob moved into his current role as a Senior Scientist in 2013, where he developed methods for a range of applications including vitamin D metabolites, vitamin B1 and B6 metabolites and a large panel of steroid hormones. Rob have presented work at conferences worldwide, gaining my first publication in Clinica Chimica Acta as well as having a patent application pending.
</blockquote>

Waters Corporation: Automating Toxicology LC/MS workflows – Improving productivity and analytical performance through automation

Automating Toxicology LC/MS workflows – Improving productivity and analytical performance through automation

This presentation will describe how automation of LC/MS methods can improve productivity and analytical results.

Automating Toxicology LC/MS workflows

<h3>Top 3 takeaways:</h3>
<ul>
<li>The ability to detect and quantify fluticasone propionate and salmeterol xinafoate down to 0.050 pg/mL when using the SCIEX 7500 system, in a human plasma matrix
</li>
<li>Excellent levels of precision and accuracy at every concentration level analysed
</li>
<li>Ultra-low level quantification within an 8 minute run time, delivering high quality data along with excellent throughput
</li>
</ul>
<blockquote>
Presenter: Jack Steed (Technical Specialist, SCIEX)
</blockquote>

SCIEX: How to determine very low pg/ml LLoQ in human plasma of anti-asthmatic drugs

How to determine very low pg/ml LLoQ in human plasma of anti-asthmatic drugs

The ability to detect and quantify fluticasone propionate and salmeterol xinafoate down to 0.050 pg/mL in a human plasma matrix

Very low pg/ml LLoQ in human plasma of anti-asthmatic drugs

Presentation Topics
<ul>
<li>Origins of the Digital Ion Trap
</li>
<li>Ion Trap v. Digital Ion Trap
</li>
<li>Shimadzu&#39;s Journey to the MALDI Digital Ion Trap
</li>
<li>Basic Proteomics
</li>
<li>Glycomics
</li>
<li>Antibody Drug Conjugates
</li>
<li>Summary and Acknowledgements
</li>
</ul>

Shimadzu: Proteomic Analysis Using a Miniature MALDI Digital Ion Trap Mass Spectrometer

Proteomic Analysis Using a Miniature MALDI Digital Ion Trap Mass Spectrometer

Proteomic Analysis Using a Miniature MALDI Digital Ion Trap

The hunt for novel drug candidate is a tedious and time-consuming process. A good workflow efficiency, from start of chemistry to purification to structural elucidation should streamline the discovery process and increase the success rate of developing novel candidate compound.
In this Mass Discussion, Dr. Ramesh Appadurai from Syngene International and Pottayil G Sasikumar from Aurigene Discovery Technologies will share their experience in running successful R&amp;D pipeline with the latest technology advancement. Let us also take a brief glimpse of Shimadzu&#39;s innovative drug discovery solutions which are designed to provide comprehensive and holistic workflow solution, ensuring faster result generation and maximizing ROI
<h5>Learning Objectives:</h5>
<ul>
<li>Analytical approaches for successful and innovative drug discovery pipeline
</li>
<li>Shimadzu&#39;s unique Drug Discovery Workflow
</li>
<li>Peptide-based drug candidates for cancer immunotherapy
</li>
</ul>
<blockquote>
Presenter: Dr Ramesh Appadurai (Research Director – Discovery Analytical Chemistry, Syngene International)
</blockquote>
<blockquote>
Dr Ramesh Appadurai Research Director and Head of Discovery Analytical Chemistry’ for Syngene International, a Contract Research Organization [CRO] company. He has over 16 years plus experience in this field Discovery Analytical Chemistry. Being in Discovery Analytical Lab, his key expertise is in developing efficient purification workflows.
</blockquote>
<blockquote>
Presenter: Dr. Pottayil G Sasikumar (Independent consultant and Advisor - Peptide Chemistry, Aurigene Discovery Technologies Limited, Bangalore)
</blockquote>
<blockquote>
Dr. Pottayil G Sasikumar has more than 15 years of experience in peptide therapeutics and peptide/amino acid inspired small molecule drug discovery. He joined Aurigene Discovery Technologies Limited in 2006, and established the peptide department. As Research Director, he headed various aspects of peptide therapeutics including the lead optimization and CMC activities. His research mainly focuses on innovative chemical biology research fundamentally linked to outstanding immunological questions, to create new solutions for immune-related diseases. He is the co-inventor of an anti-cancer drug CA-170 (First orally bioavailable checkpoint inhibitor- Immuno-oncology agent) currently in Phase 2b/3 clinical trials and several other drugs which are in various phases of clinical development. 
</blockquote>
<blockquote>
Presenter: David Chan (Assistant Product Manager, Shimadzu (Asia Pacific) Pte. Ltd)
</blockquote>
<blockquote>
As LC Product Manager, David Chan is based in Shimadzu Asia Pacific, managing and handling the LC product line in the region. Before joining Shimadzu Asia Pacific, David was a Drug Discovery Chemist with more than 10 years of experience in small molecule design and synthesis. He understands the day-to-day difficulties faced by the synthetic chemist well through his extensive working experience, and knows how to apply the correct technology advancement to overcome these issues, in order to streamline the tedious discovery process.
</blockquote>

Shimadzu: Toolkit for Treasure Hunt - Drug Discovery Made Easy

Toolkit for Treasure Hunt - Drug Discovery Made Easy

Running successful R&D pipeline with the latest technology advancement.

The COVID-19 pandemic has brought unprecedented challenges for the communities and economies worldwide, causing a profound effect on the lives of billions of people.
While government and communities are trying to contain the spread, researchers are aggressively focusing their efforts towards finding a treatment and/or vaccine. Science is more important now than ever before.
This webinar will feature the current analytical technologies and solutions that Shimadzu is committed to provide – such as analytical techniques for drug testing and vaccine research, production and testing of sanitizers and personal protective equipment and analysis of medical sewage waste.
<h5>Learning Objectives:</h5>
<ul>
<li>Comprehensive workflow for Pharma, PPE manufacturing and Environmental monitoring
</li>
<li>Explore solutions accelerating research
</li>
<li>Informatics workflows enabling remote operations and monitoring
</li>
</ul>
<blockquote>
Presenter: Sandhya (Sandy) Nargund (Deputy General Manager, Shimadzu (Asia Pacific) Pte. Ltd.)
</blockquote>
<blockquote>
Sandy has over 22 years’ experience in Analytical field with various roles starting from Pharma QC to Mass Spectrometry expert in supporting advance MS technologies. Sandy has wide experience on Mass Spectrometry while working as ‘Application Specialist’ and has conducted many users training and workflow trainings for small molecules and proteomics using LC-MS/MS, GC-MS/MS and High-resolution MS. She is currently working as Deputy General Manager in Shimadzu Asia Pacific, Singapore and is responsible for managing and promoting Shimadzu&#39;s analytical products range including Spectroscopy, Chromatography and Mass Spectrometry in region and to provide technical support on various applications in food safety, Pharma, Clinical and other markets.
</blockquote>
<blockquote>
Presenter: Jackie (Product Specialist (GC &amp; GCMS), Shimadzu (Asia Pacific) Pte. Ltd.)
</blockquote>
<blockquote>
After graduating from the National University of Singapore, Jackie has garnered over 6 years of research, training and facilitation experience. Jackie meets scientists from various industries to share technical knowledge and collect feedback, understanding their problems and helping them find well-suited solutions in GC-related analyses.
</blockquote>

Shimadzu: Analytical Technologies for COVID-19 Challenges: Drug Repurposing | Vaccine Research | PPE & Material Testing | Environmental Monitoring

Analytical Technologies for COVID-19 Challenges: Drug Repurposing | Vaccine Research | PPE & Material Testing | Environmental Monitoring

The current analytical technologies and solutions that Shimadzu is committed to provide.

Analytical Technologies for COVID-19 Challenges

There is a shifting trend in the analytical platform from immunoassay to mass spectrometry in clinical research and diagnostic lab in the past decade.
This growing trend of mass spectrometry application in clinical lab is due to the superior selectivity and sensitivity of mass spectrometers. More importantly, mass spectrometry can analyse multiple compounds in a single analysis using much lesser sample amount compared to immunoassay. The application of mass spectrometry in various clinical analysis using Shimadzu’s Ultra Fast Mass Spectrometry (UFMS) Technology will be discussed in this webinar. Lastly, Shimadzu latest innovative technology in clinical automation and rapid screening will also be highlighted.
Learning Objectives:
<ul>
<li>Introduction, background and advantages of mass spectrometry in clinical labs
</li>
<li>Understand how you can use LC-MS/MS in various clinical analyses
</li>
<li>Understand Shimadzu’s latest innovative technology in clinical automation and rapid screening.
</li>
</ul>
<blockquote>
Presenter: Daryl Hee (Ph.D.) (Senior Product Specialist (LCMS), Shimadzu (Asia Pacific) Pte Ltd)
</blockquote>
<blockquote>
Daryl Hee is currently the Senior Product Specialist for LCMS at Shimadzu (Asia Pacific) Pte Ltd. He completed his PhD study at the National University of Singapore (NUS) in 2010. Following that, he continued his postdoctoral research at the Department of Medicine NUS in clinical trial pharmacokinetics studies and conducted lectures on drug and metabolite analysis using LCMS. He is currently also holding the Adjunct Senior Research Fellow position with NUS medicine Department.
</blockquote>

Shimadzu: Brace Yourselves: The Shift to Mass Spectrometry for Clinical Analysis

Brace Yourselves: The Shift to Mass Spectrometry for Clinical Analysis

The application of mass spectrometry in various clinical analysis using Shimadzu’s Ultra Fast Mass Spectrometry (UFMS) Technology.