In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with Imad Haidar Ahmad, Scientific Research Director at Amgen leading separations for Discovery Chemistry and Externalization. Imad's career spans Novartis, Merck, and now Amgen, and his research on computer-assisted method development has landed multiple front covers in Analytical Chemistry.We dig into how his group builds retention models from as few as nine lab experiments to map an entire separation landscape — replacing weeks of trial and error with a resolution map you can query and rebuild from. Imad breaks down where this modeling approach struggles (large molecules, SFC, fully autonomous method development), walks through two-dimensional liquid chromatography in plain terms for anyone who's only worked in 1D, and shares the story behind his group's greenness-score mapping work — including where swapping acetonitrile for methanol worked, and where it didn't.We also talk career advice: what a typical day looks like leading a discovery-stage separations team, how undergrad and grad students can bridge the academia-to-industry gap, and what Imad sees as the field's biggest unsolved problem.<h3>Video Transcription</h3>Chromatographic method development is increasingly moving beyond trial and error toward workflows built on modeling, automation, multidimensional separations, and smarter use of data. In a recent interview, Haidar Ahmad discussed how these approaches are changing pharmaceutical analysis, why understanding the fundamentals of chromatography remains essential, and where some of the biggest opportunities in separation science still lie.Ahmad’s route into analytical chemistry was not entirely planned. As an undergraduate, he initially found inorganic and organic chemistry particularly attractive, but a final-year project focused on the analysis of additives in bread introduced him to the possibilities of analytical chemistry. During his PhD, his research moved into polymer analysis and structure–property relationships. His interest in chromatography deepened when he encountered two-dimensional liquid chromatography (2D-LC), particularly its ability to reveal forms of polymer heterogeneity that could not be adequately characterized using a single separation technique. This eventually led to postdoctoral work with Peter Carr, where Ahmad focused on chromatographic separations and the fundamentals of chromatography.His subsequent career in the pharmaceutical industry exposed him to different stages of drug development, from late-stage method development and validation to drug substance characterization, purification, and finally discovery-stage separations. That breadth of experience has shaped his view of chromatography as both a scientific discipline and a practical problem-solving tool. In late-stage development, the objective may be a highly robust method capable of successful validation and transfer between laboratories. In discovery, by contrast, speed becomes critical because teams may be working with thousands of molecules while trying to identify the best candidates to advance.&nbsp;<h4>Discovery-stage chromatography: speed, prioritization, and throughput</h4>For a separations team supporting drug discovery, the working day is shaped by a constant flow of requests from medicinal chemistry and other partner groups. The challenge is not simply to perform the analyses, but to decide which requests are most urgent, remove bottlenecks, communicate with project teams, and continuously improve existing workflows.According to Ahmad, one of the most important questions is whether a task can be done faster or in a more high-throughput way. Discovery laboratories operate under strong time pressure, and efficiency therefore becomes part of the scientific strategy rather than merely an operational concern. As he describes it, the work combines science, prioritization, leadership, and communication, all with the aim of moving the most important projects forward as quickly as possible.<h4>In silico modeling: letting the computer perform the experiments</h4>One of the central themes of Ahmad’s work is in silico chromatographic modeling. In practical terms, the approach begins with a carefully designed set of laboratory experiments—often around nine runs. The resulting data are fed into software that builds retention models describing how analyte retention changes with variables such as mobile-phase composition and temperature.The software can then generate a resolution map representing a much larger separation space than could realistically be tested experimentally. Instead of running dozens or hundreds of conditions by trial and error, the analyst can identify regions where critical peak pairs are predicted to achieve the best separation.For Ahmad, the value of these maps goes beyond simply finding the condition with the highest resolution. Each point on the map represents a chromatographic condition, while the visual output shows where poor and strong separations are expected. A broad region of good resolution is particularly useful because it points toward a more robust operating space rather than a narrowly defined optimum that might be sensitive to small variations in experimental conditions.He therefore prefers to think of a chromatographic model as a map or database, rather than simply an optimization tool. If an impurity later disappears from the process, the corresponding peak can be removed from the model and new conditions selected without repeating the complete experimental study. Conversely, if a new degradation product appears during stability studies, the peak can be added and the existing model used to search for updated separation conditions.<h4>Why trial and error still has a place</h4>Modeling does not mean that conventional trial-and-error method development has disappeared. Ahmad points out that it remains useful in early discovery, particularly for fit-for-purpose methods where speed is more important than achieving a fully optimized separation.The situation changes later in development. A method intended for validation, transfer, or long-term routine use carries much greater risk. Trial and error may identify a condition that appears satisfactory without revealing whether it represents the most robust region of the separation space. If that method later fails during transfer or routine operation, the consequences can be costly.For late-stage methods, the additional investment required to build a model can therefore reduce risk over the much longer lifetime of the analytical procedure.<h4>Models are only as good as the experiments behind them</h4>Chromatographic modeling is not automatic proof against poor method development. Ahmad compares the technology to a GPS: it is highly useful, but only if the analyst understands where they are trying to go.Careful experimental design remains essential. Incorrect dwell-volume or column-volume information, as well as insufficient column re-equilibration, can produce misleading predictions. Importantly, these problems may not be obvious when the experimental runs used to construct the model are compared with their calculated values. They may emerge only when the software begins predicting new conditions within the modeled design space.Modeling is also easier for some separations than others. Ahmad describes reversed-phase LC of small molecules as comparatively straightforward, while large biomolecules are more challenging because their retention can be highly sensitive to small changes in mobile-phase composition and temperature.Other separation modes present additional difficulties. Ahmad notes that his teams have found supercritical fluid chromatography (SFC) particularly challenging to model successfully. Fully autonomous method development remains another unresolved goal: current tools can automate parts of optimization, but analyst input is still required.<h4>Why two dimensions can reveal what one dimension cannot</h4>Ahmad’s early interest in 2D-LC came from polymer characterization. He explains the principle simply: one-dimensional chromatography may answer one question about a sample, while two complementary dimensions can answer two.Two copolymers, for example, may have similar molecular size and therefore coelute in size-exclusion chromatography. A second separation mechanism based on interaction with a stationary phase may distinguish them according to chemical composition. Combining both dimensions provides a more complete description of complex distributions involving molecular weight, composition, sequence, and other forms of heterogeneity.Historically, the complexity of both instrumentation and software limited wider adoption of 2D-LC. Ahmad recalls systems requiring several computers and multiple software packages. That situation has improved substantially, and 2D-LC is now easier to implement across research and pharmaceutical development.Nevertheless, modeling multidimensional separations still presents challenges. While models can be built for individual fractions or dimensions, current software does not easily identify a common optimum across multiple models simultaneously. Much of this comparison still requires manual work.<h4>Bringing sustainability into method development</h4>Another area where Ahmad sees room for change is green analytical chemistry.Traditional pharmaceutical method development has understandably prioritized resolution, sensitivity, robustness, and turnaround time. Sustainability has often been treated as a secondary consideration, particularly at the analytical scale, where solvent use appears small compared with preparative chromatography or manufacturing.That mindset is beginning to change. Ahmad argues that greenness metrics should increasingly be incorporated directly into method-development software. If analysts could immediately see how changes in flow rate, gradient conditions, solvent choice, or other parameters affect the environmental profile of a method, sustainability could become part of routine optimization rather than an assessment performed after the method has already been developed.The objective, however, is not to pursue the greenest possible method regardless of analytical performance. Ahmad emphasizes the need to develop methods that are as green as possible while remaining fit for purpose. Modeling can potentially help balance these competing requirements by identifying conditions that preserve separation performance while allowing greener solvents or alternative chromatographic conditions to be introduced.<h4>Advice for young chromatographers: do not treat HPLC as a black box</h4>For students learning chromatography, Ahmad’s central recommendation is straightforward: do not treat the HPLC system as a black box.Rather than collecting chromatograms by making random changes, he encourages analysts to build a mental model of the separation. When temperature, gradient, or another parameter is changed, they should ask why retention or selectivity changed and use each experiment to improve their understanding of the system.He also recommends making use of educational resources on chromatographic fundamentals and method development and, importantly, not being afraid to experiment with instrument conditions. Modern systems are robust, but the analyst still needs to understand the underlying separation science in order to use them effectively.The same proactive attitude applies to career development. Ahmad strongly encourages students to seek industry internships and make connections beyond their own institutions. Conferences and poster sessions can provide natural opportunities to meet scientists from industry, discuss research, and ask about internship opportunities. Based on his own experience working with undergraduate and graduate interns, these collaborations can benefit both students and research teams and may strongly influence later academic or career decisions.<h4>What still needs to be solved?</h4>Asked about the biggest unresolved challenges in separation science, Ahmad does not begin with technology. Instead, he points to a shortage of well-trained analytical chemists with a strong understanding of chromatographic fundamentals.Alongside education, he identifies several technical opportunities. One is truly autonomous method development, where an instrument and software could optimize a separation without continuous analyst input. Another is the ability to predict chromatographic selectivity directly from molecular structure.Such capability could be especially valuable in high-throughput discovery environments. If a laboratory could predict which stationary phase and conditions were most likely to separate a particular set of compounds or isomers before entering the laboratory, method development could become considerably faster and more efficient.Taken together, Ahmad’s perspective points toward a future in which chromatography becomes increasingly model-driven, automated, multidimensional, and sustainability-aware. But the technology does not eliminate the need for chromatographic knowledge. On the contrary, as analytical tools become more powerful, understanding the fundamentals may become even more important—the software can explore the separation space, but the scientist still needs to know what question to ask and how to interpret the answer.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 In Silico Modeling, 2D-LC & Green Chromatography: Imad Ahmad on the Future of Method Development

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

Researchers from CATRIN at Palacký University, together with colleagues from VSB – Technical University of Ostrava and Charles University, have developed a new graphene ink for producing inexpensive and sensitive printed sensors. Using single-atom engineering, they incorporated copper atoms into the material, enabling highly sensitive detection of dopamine – a neurotransmitter responsible for transmitting signals between nerve cells. The approach, <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">presented in the journal Advanced Science</a>, has strong potential for use in medical diagnostics.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp 117w," sizes="100vw" width="1920" height="2560">Single-atom engineering makes it possible to control the chemical reactivity of materials at the level of individual atoms. Its broader application in medical diagnostics has so far been limited by a lack of atomically engineered materials and printable inks based on them. Researchers have now developed a new class of single-atom-doped graphene materials as well as an ink for producing sensitive printed sensors. Copper proved particularly effective for dopamine detection, which is important for biomedical research and the development of advanced diagnostic technologies for diseases including Alzheimer’s disease.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp 117w," sizes="100vw" width="1920" height="2560">“The NGA-Cu ink we developed enables the production of fully inkjet-printed, sustainable paper-based electrodes at very low cost. Each sensor requires only 1.3 micrograms of active ink, costs approximately one Czech koruna and provided a stable response for at least three months. The ink is also fully compatible with commonly available materials inkjet printers. This creates significant potential for producing affordable, digitally designed sensors whose function can be tailored atom by atom,” said&nbsp;the corresponding author of the <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">paper</a> Michal Otyepka.The basis for this new generation of single-atom inks was nitrogen-doped graphene acid (NGA), which CATRIN researchers developed several years ago. They have now progressively enriched it with different individual metal atoms, including copper, iron and manganese.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp 117w," sizes="100vw" width="1920" height="2560">“We found that the electrochemical response depends fundamentally on the type of atom used. While copper atoms significantly enhance the dopamine signal, other metals suppress it. What matters, therefore, is not only the presence of isolated metal atoms but also the specific metal involved. This is a very important finding for the further development of biosensors,” explained first author Martin-Alex Nalepa.The published results introduce a new class of sensing materials with considerable potential for medical diagnostics. The researchers present a broadly applicable platform for developing graphene inks containing individual metal atoms and open the way towards scalable, reproducible and material-efficient production of advanced electrochemical sensors.&nbsp;“We are only at the beginning and are fascinated by the new possibilities opening up before us. We can see that by changing the type of atom, we can control the sensitivity and selectivity of the sensor. In addition to dopamine, we can also determine immunoglobulins very precisely – antibodies whose levels and types may serve as important indicators of autoimmune or infectious diseases,” emphasised Radek Zbořil, a co-author of the paper.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp 117w," sizes="100vw" width="1920" height="2560">Electrochemical biosensors can find applications not only in medicine but also in food quality control, drinking-water monitoring and environmental pollution monitoring. Their key components are functionalised electrodes, and graphene-based materials have proved particularly suitable for their production. A key factor in the success of the Olomouc researchers was the targeted preparation of graphene ink with the required properties.The research was supported by the TECHSCALE project under the Johannes Amos Comenius Programme.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics: Project TECHSCALE (No. CZ.02.01.01/00/22_008/0004587) is financed by the MEYS OP JAC Excellent research programme, supported by ERDF/ESF." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg 245w," sizes="100vw" width="620" height="87"><blockquote><h4>Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">Single-Atom-Enhanced Fully Inkjet-Printed Electrochemical Sensor for Dopamine Detection</a></h4>Martin-Alex Nalepa, David Panáček, Vítězslav Hrubý, Matěj Jendřišák, Rostislav Langer, Michal Langer, Petr Jakubec, Ivan Dědek, Vojtěch Kupka, Michal Mazur, Radek Zbořil, Michal OtyepkaAdv. Sci., 2026, e76796<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/advs.76796">https://doi.org/10.1002/advs.76796</a><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Single-atom (SA) engineering offers atomic-level control over interfacial reactivity, yet the lack of printable SA-based inks hinders its practical translation into electrochemical sensing. Here, a fully water-based inkjet-printable ink is introduced based on nitrogen-doped graphene acid (NGA) hosting atomically dispersed Cu centers (NGA-Cu-ink). The ink enables digitally controlled, spatially defined deposition and fabrication of low-cost ($0.04 per sensor), fully inkjet-printed sustainable electrodes on paper. A comparison of NGA functionalized with different SA dopants (Cu, Mn, Fe, Ce) identifies a strong dopant-dependent electrochemical response, with Cu uniquely enhancing the analyte signal while other dopants suppress it, demonstrating that SA identity is a decisive design parameter in printed sensing interfaces profiling sensitivity and selectivity. The functional role of the NGA support is to provide dense anchoring sites (nitrogen and carboxylate groups) that stabilize atomically dispersed metal centers and create adsorption- and electron-transfer–active microenvironments. The NGA-Cu-ink yields enhanced dopamine oxidation, enabling quantitative detection on fully printed devices (limit of detection 9.7 µM; linear range 50–400 µM) and printed-on-electrode platforms (10.6 µM), while maintaining &lt;10% signal variation over 11 weeks. The approach establishes a general route to single-atom graphene-based inks for scalable, reproducible, and low-material-consumption manufacturing of advanced electrochemical sensors.

Atom by atom. Scientists reveal a new direction in medical diagnostics

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

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Agilent Technologies

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Agilent Technologies: SureMass: Fast, Accurate, and Reproducible Data Processing

SureMass: Fast, Accurate, and Reproducible Data Processing

In this webinar SureMass conversion will be shown and examples of its speed and improved mass accuracy will be shared.

Digital transformation is a journey driven by the desire to fundamentally improve laboratory working practices by leveraging the latest advances in technology. Whether it be a need to eliminate errors, operate more efficiently, or reach confident results faster, laboratories are focusing more than ever on software.
Designed as a scalable solution for modern, dynamic (often cloud-based) environments, the Thermo Scientific™ Ardia™ Platform brings together chromatography and mass spectrometry, connecting data and instruments, helping scientists get closer to the truth. In this webinar, discover how you can reap the benefits of a connected platform to maximize instrument utilization, streamline lab and data management, and realize true efficiency gains.
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SelectScience: Take control of your data and instruments: Your journey starts here

Take control of your data and instruments: Your journey starts here

The Thermo Scientific Ardia Platform brings together chromatography and mass spectrometry, connecting data and instruments.

Take control of your data and instruments: Your journey

Identifying fentanyl analogs and other novel psychoactive substances (NPS) in the recreational drug market poses a significant analytical challenge for forensic toxicology laboratories.
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</ul>
<blockquote>
Presenter: Dr. Aaron Stella (R&amp;D Scientist, SCIEX)
</blockquote>
<blockquote>
Presenter: Dr. Karl Oetjen (Senior Scientist, Technical Marketing, SCIEX)
</blockquote>

Uncovering Novel Psychoactive Substance Biomarkers Using Diagnostic Fragment Screening

We will showcase how accurate mass diagnostic fragments of fentanyl and other analogs can be utilized to identify new fentanyl analog biomarkers.

NPS Biomarkers Using Diagnostic Fragment Screening

Purification is critical to many scientific workflows. Compounds of interest are rarely found to be the right purity in their native state. Join Waters and Virscidian for this 6-part webinar series to learn the basics of purification and application of purification principles for more advanced workflows.
<h3>PART 2 | Basics of Supercritical Fluid Chromatography Purification</h3>
Supercritical Fluid Chromatography (SFC) is an effective and green technique to isolate a compound(s) of interest from a mixture. SFC utilizes liquid CO2 to quickly isolate compounds while minimizing the efforts required to dry the collected fraction. In this module, we will discuss factors involved in SFC purification. 
Learning Objectives: 
<ul>
<li>Understand how to use SFC purification</li>
<li>Make informed decisions on method optimization</li>
<li>Understand impact of decisions made during purification on larger workflow</li>
</ul>
<blockquote>
Presenter: Andrew Leightner (Market Development Manager – Americas Field Marketing, Waters Corporation)
</blockquote>
<blockquote>
Andy Leightner has over 10 years of experience serving the pharma market from contract research organizations. He specialized in method development and validation leveraging LC, GC, and mass spectrometry to meet complex client needs. He supports pharmaceutical and biopharmaceutical discovery and development in the Americas for Waters Corporation. Andy received a bachelors degree in biology from the University of Wisconsin-Madison.
</blockquote>

Waters Corporation: Basics of Supercritical Fluid Chromatography Purification

Basics of Supercritical Fluid Chromatography Purification

We will discuss factors involved in SFC purification.

Analysis of non-derivatized polar pesticides in food is a complex task due to their physicochemical properties. Their high polarity makes them incompatible with universal extraction techniques such as QuEChERS whilst limiting retention on classical reversed-phase chromatography columns. Additionally, loss of analyte due to adsorption by metals in the LC flow path can further compromise both chromatographic peak shape and analytical sensitivity.
In this presentation we describe a comprehensive verified LC/MS/MS workflow, from sample preparation to MS detection for the simultaneous quantitation of 11 underivatized polar pesticides in five matrices adhering to the to SANTE 11312/2021 guidelines.
What you will learn:
<ol>
<li>Sample preparation according to the QuPPE method</li>
<li>How to optimize, LOQ, linearity, recovery, and precision</li>
<li>How to assess matrix effects</li>
<li>Practical ‘Tips and Tricks’ to ensure effective implementation of this workflow in your lab</li>
</ol>
<blockquote>
Presenter: Anna Cali (Agilent Technologies, Milan, Italy)
</blockquote>
<blockquote>
Anna Calì has nearly two decades of industry experience in developing methods for the analysis of pesticides, mycotoxins and contaminants using both liquid phase and gas phase mass spectrometry (LC/MS and GC/MS). Prior to joining Agilent Technologies as an LC/MS and GC/MS Application Engineer in 2011, she worked as an analytical chemist in both public and private laboratories as well as an Application Engineer in global mass spectrometry companies.
</blockquote>

Agilent Technologies: A Robust Method For the Sensitive Analysis of Non-Derivatized Polar Pesticides in Food by LC/MS/MS

A Robust Method For the Sensitive Analysis of Non-Derivatized Polar Pesticides in Food by LC/MS/MS

LC/MS/MS workflow, from sample preparation to MS detection for the simultaneous quantitation of 11 underivatized polar pesticides in five matrices.

Non-Derivatized Polar Pesticides in Food by LC/MS/MS

We are eagerly looking forward to this year’s ASMS, the biggest annual congregation of scientists working on mass spectrometry. This year the conference is held in Houston, Texas and we cannot wait to meet you and share our latest projects with you.
You can find us throughout the week at our orange Evosep corner at booth #712, where we would be thrilled to connect with you and exchange information about your ongoing research. Furthermore, we are delighted to introduce you to new advancements of our technology, and additionally, we will also host our User Meeting, Breakfast Seminar and Hospitality Suite, where we guarantee that this year’s special theme will exceed your expectations!
<h5>ASMS 2023 Posters:</h5>
TP 642 – Next-generation biotyping of microorganisms for assessment of antibiotics resistance and virulence factors
TP 152 – A complete, automated end-to-end workflow for high-throughput therapeutic drug monitoring using Evosep MRM
TP 566 – High throughput quantification of biologics PTM state by peptide mapping using refocussing chromatography and ZenoTOF mass spectrometry
WP 655 – Pipetting-free single cell analysis with the label-free proteoCHIP and the Evotip adapter for high sensitivity proteomics on the timsTOF SCP
TP 156 – High-throughput end-to-end automated Evosep-MRM workflow for the quantification of glycated albumin in diabetes
ThP 421 – A complete and automated end-to-end sample preparation strategy for high-throughput and standardized proteomics with high sensitivity
WP 656 – Pushing the boundaries for robust and high-throughput single cell analysis with Whisper Flow technology powered by dia-PASEF

EVOSEP: ASMS 2023 highlights: Automating end-to-end workflows

ASMS 2023 highlights: Automating end-to-end workflows

Get an overview of all the highlights of Evosep at ASMS 2023, including new research, talks and much more from the conference!

Axcend Focus LC Free Webinar

What's New in HPLC?

Axcend Focus LC Free Webinar by Axcend team.

<h5>Key learning objectives:</h5>
<ul>
<li>What is chiral chromatography, chiral screening, why do it, what are the benefits...</li>
<li>What the chiral screening process looks like</li>
<li>Scope, deliverables, and timelines for chiral screening</li>
<li>How to request chiral screening services</li>
</ul>
<blockquote>
Presenter: Zandra Baja (Applications Scientist)
</blockquote>
<blockquote>
Zandra is an Applications Scientist at PhenoLogix specializing in chiral screening, USP and PhEur applications, and environmental GC-MS applications. Zandra obtained her bachelor&#39;s degree in chemistry from California State University of Long Beach. Prior to joining Phenomenex in 2021, she spent 3 years working as a chemist in the petroleum industry and 8 years as an analytical chemist working with gas chromatography in an environmental air testing laboratory. Zandra has also spent several years in quality assurance management where she was responsible for maintaining ISO/IEC 17025 and TNI Standard laboratory accreditations. 
</blockquote>

Phenomenex: Chiral Chromatography Screening 101

Chiral Chromatography Screening 101

What is chiral chromatography, chiral screening, why do it, what are the benefits.... What the chiral screening process looks like....

Understanding the biophysical state, quality and properties of biopharmaceuticals is essential in product and process development. However, available material, time and resources are often limiting.
Dynamic light scattering (DLS) is an industry standard technique for protein formulation, particle sizing and stability studies. This webinar will illuminate how high-throughput DLS (HT-DLS), performed in low-volume microwell plates, is used to understand aggregation as well as colloidal and thermal stability of therapeutic protein candidates and formulations. Application examples include various studies of freeze-thaw, formulation buffer screening and thermal stability.
Key Learning Objectives:
<ul>
<li>Background and instrumentation for HT-DLS</li>
<li>How DLS is used to study aggregation under applied stresses</li>
<li>How DLS evaluates protein colloidal stability via second virial coefficient A2 and the diffusion interaction parameter kD</li>
</ul>
Who Should Attend:
<ul>
<li>Biopharma product development scientists in need of automated methods for characterizing protein stability</li>
<li>Formulation lab managers seeking to improve productivity with high-throughput DLS instrumentation</li>
</ul>
<blockquote>
Presenter: Felix Gloge, Ph.D. (Field Application Scientist, Wyatt Technology)
</blockquote>
<blockquote>
Presenter: Christian Sieg, Ph.D. (Application Scientist, Wyatt Technology)
</blockquote>
<blockquote>
Presenter: Melissa O&#39;Meara (Forensic Science Consultant, C&amp;EN Media Group)
</blockquote>

C&EN: Analyzing Protein Developability and Stability at High-Throughput

Analyzing Protein Developability and Stability at High-Throughput

How high-throughput DLS (HT-DLS), performed in low-volume microwell plates, is use to understand aggregation, colloidal and thermal stability of protein

Protein Developability and Stability at High-Throughput

GPC/SEC is the go-to technique for determining the molecular weight distribution of synthetic and natural polymers and for comparing batch to batch quality. Specific criteria should be considered when developing or optimizing your method to ensure the success of the application: 
<ul>
<li>Why determining molecular weight is important</li>
<li>Key parameters for solvent and column selection</li>
<li>Column type options, their features, and benefits</li>
<li>Importance of polymer standards selection and calibrating</li>
<li>Modes of Detection and expanding the information for your polymer</li>
</ul>
Practical steps, helpful product information, and application examples will be provided to assist you with selecting the best GPC/SEC solution for your polymer sample. 
Key Learning Objectives:
<ul>
<li>How to select the best GPC/SEC solution for your polymer sample</li>
<li>Learn practical tips and tricks to optimize your GPC/SEC workflow</li>
<li>Best practices for polymer analysis</li>
</ul>
Who Should Attend:
<ul>
<li>Chromatographers interested in polymer analysis</li>
</ul>
<blockquote>
Presenter: Jean Lane (Applications Engineer, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Jeff Huber (Contributing Editor, C&amp;EN Media Group)
</blockquote>

C&EN: Have You Got the Chromatography Tools You Need for Polymer Analysis?

Have You Got the Chromatography Tools You Need for Polymer Analysis?

Practical steps, helpful product information, and application examples will be provided to assist you with selecting the best GPC/SEC solution.