What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

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<h3>Solving Problems That Matter - a Waters webinar series featuring USP speakers</h3>
<h4>Episode 3: Enhanced Method Development</h4>
Summary
Enhanced method development is a science and risk-based approach to analytical procedure development that leverages QbD principles and “starts with the end in mind”. Our panelists Amanda Guiraldelli from the USP and Richard Verseput form S-Matrix will share their insights on approaches outlined in the ICH Q14 quality guidelines and USP general chapter &lt;1220&gt;. 
Key learning objectives:
<ul>
<li>Discover the benefits of the enhanced method development approach</li>
<li>Learn how to implement analytical QbD approaches in your lab</li>
</ul>
Who should attend:
Pharma scientists involved with analytical LC method development
<blockquote>
Presenter: Amanda Guiraldelli, Ph.D. (Scientific Affairs Manager, U.S. Pharmacopoeia)
</blockquote>
<blockquote>
Amanda Guiraldelli has been with USP since 2012 and holds the position of scientific affairs manager and principle scientist in the compendial science group-general chapters. She also serves as scientific liaison for the USP Measurement and Data Quality Expert Committee, where she works to develop and revise USP standards related to analytical procedure lifecycle/validation. Previously, Amanda worked as senior scientist at the USP reference standard laboratory for 8 years with characterization of compendial standards. She is visiting professor at the Institute of Chemistry at the University of Campinas (UNICAMP) in Brazil and is a frequent speaker and instructor on topics related to analytical procedure life cycle, Analytical Quality by Design (AQbD) and Chromatography. Amanda is specialist in mass spectrometry, chromatography, omics and chemometrics and has more than 15 years of experience in pharmaceutical R&amp;D areas. Prior to joining USP, she was R&amp;D scientist in a brazilian pharmaceutical industry and visiting scientist at TU Berlin in Germany and Leiden University in Netherlands (Center for Proteomics and Metabolomics) working on proteins characterization by LC-HRMS and procedure development using UHPLC-HRMS. Amanda is graduated in pharmacy biochemistry and holds a Ph.D. in analytical chemistry from the FCFRP - University of São Paulo (metabolomics by UHPLC-HRMS, GC-MS and 1H NMR and chemometrics).
</blockquote>
<blockquote>
Presenter: Richard Verseput (President, S-Matrix)
</blockquote>
<blockquote>
Richard Verseput has over 30 years of experience applying QbD tools and methods in multiple industries. For the last 18 years he has worked with analytical instrument manufacturers, regulatory agencies, and global pharma customers to develop and advance automated QbD experimentation. Richard Verseput is currently president of S-Matrix – the developer of Fusion QbD® – a regulatory compliance supporting software platform which automates QbD experimentation, peak tracking, and chromatography data modeling for the successful development, validation, and transfer of LC and LC-MS methods.
</blockquote>
<blockquote>
Moderated by: Isabelle Vu Trieu (Pharmaceutical Market Development Manager, Americas, Waters Corporation)
</blockquote>
<blockquote>
Isabelle is a business development professional dedicated to delivering benefits to analytical scientists in the life sciences industry. With over 20 years of experience in providing chromatography and mass spectrometry solutions, Isabelle is particularly interested in helping pharmaceutical scientists achieve the data quality and integrity needed to drive decisions for drug quality, safety and efficacy. Isabelle joined Waters in 2009 and currently manages the development of the pharmaceutical market in the Americas. She holds Masters’ degrees in Chemistry from Columbia University and the University Claude Bernard in Lyon, France as well as a Chemical Engineering degree from Ecole Supérieure de Chimie Physique Electronique in Lyon, France.
</blockquote>

Waters Corporation: Solving Problems That Matter - a Waters webinar series featuring USP speakers

Enhanced Method Development

Solving Problems That Matter - a Waters webinar series featuring USP speakers. Episode 3: Enhanced Method Development.

Synthetic and naturally occurring macromolecules are often complex mixtures of many molecules differing in molar mass, as well as in other structural features such as comonomer content and distribution, branching, or end-group functionalization. One-dimensional separation methods like size exclusion chromatography (SEC) or interaction chromatography (IC) are usually not sufficient to resolve such complex mixtures due to limitations of the separation mechanisms. Given the multidimensional complexity of such samples, hyphenated separation methods are increasingly applied for their characterization.
In a 2D-LC separation, separation is first achieved by one feature (e.g. comonomer content) followed by a second separation by another property, such as size. This allows determination of the correlation between different structural features such as the dependence of chemical composition on molar mass.
In this webinar, our speakers will present the concept, different approaches for technical realization, and industrial applications of 2D-LC in polymer separations.
Key learning objectives
<ul>
<li>Explore the multidimensional heterogeneity of polymers</li>
<li>Learn how to perform 2D-LC separations of polymers</li>
<li>Discover additional information that can be gained by 2D-LC separations</li>
</ul>
Who should attend?
<ul>
<li>Polymer researchers</li>
<li>Scientists interested in polymer and macromolecular analysis,</li>
<li>Material scientists</li>
<li>Scientists and practitioners involved in complex separation problems</li>
</ul>
<h4>View other webinars in this series here:</h4>
Part 1: <a href="https://lcms.labrulez.com/webinar/3690">Unraveling the Intricacies of Size Exclusion Chromatography – Strength and Limitations</a>
Part 2: <a href="https://lcms.labrulez.com/webinar/3691">Detection in Gel Permeation Chromatography /Size Exclusion Chromatography</a>
Part 3: <a href="https://lcms.labrulez.com/webinar/3693">Characterization of Macromolecules by SEC-Light Scattering</a>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Wolfgang Radke (Head of Contract Analysis, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Dr. Wolfgang Radke obtained his PhD in Chemistry from the University of Mainz, Germany. He has over 30 years of experience in the field of polymer chromatography. He has significant knowledge in size exclusion chromatography, gel permeation chromatography (SEC/GPC) with conventional, MALS, and viscometry detectors . He is also experienced in polymer separations using interaction chromatography and two-dimensional chromatography. He is currently the head of the contract analysis group at Agilent Technologies.
</blockquote>
<blockquote>
Presenter: Dr. Mubasher Ahmed Bashir (Research Scientist, Dow Inc.)
</blockquote>
<blockquote>
Dr. Mubasher Bashir obtained his PhD in Chemistry from the German Institute of Plastics, DKI at TU Darmstadt, Germany. He is a research scientist and the global polymer separations technology network leader in analytical science, Core R&amp;D, at the Dow Chemical Company. He has more than 16 years of experience in analytical research and applications in the chemical industry. His current work focuses on characterizing complex polymers by employing various separation techniques (AF4, SEC, 2D-LC) and advanced detection with the goal of establishing structure property relationships to facilitate new product and process development.
</blockquote>
<blockquote>
Presenter: Jemima Arnold (Editorial Assistant, SelectScience)
</blockquote>
<blockquote>
Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: Concepts, Challenges and Applications for 2D-LC of Polymers

Concepts, Challenges and Applications for 2D-LC of Polymers

The concept, different approaches for technical realization, and industrial applications of 2D-LC in polymer separations.

When reconstructing and analyzing the tumor–immune microenvironment (TIME), exploring the spatial context of the tumor at single-cell resolution can bring new insights into the cell–cell or cell–extracellular matrix interactions.
Mapping the TIME in three dimensions offers researchers the opportunity to probe deeper into spatial cell heterogeneity in tumors and understand the impact of the TIME on cancer growth and evolution.
Join this webinar to discover how to integrate spatial biology and multiomics approaches to reveal the intricate interactions between cancer cells and their environment.
Attend this webinar to:
<ul>
<li>Learn how to gain multidimensional spatial information for disease-based research</li>
<li>Discover the impact of detecting molecular and metabolic changes in cancer cells </li>
<li>Understand the utility of mass spectrometry in assessing novel predictive biomarkers in cellular phenotypes</li>
</ul>
<blockquote>
Presenter: Professor Jared Burks (Professor/Co-Director, Flow Cytometry &amp; Cellular Imaging Core Facility, M.D. Anderson Cancer Center)
</blockquote>

Technology Networks: Multiplex Multiomics: The Future of Spatial Biology in Cancer Research

Multiplex Multiomics: The Future of Spatial Biology in Cancer Research

Discover how to integrate spatial biology and multiomics approaches to reveal the intricate interactions between cancer cells and their environment.

Multiplex Multiomics: Spatial Biology in Cancer Research

We want to Help you gain the advantages from advanced automated electrophoresis solutions. Solutions that you’ll need to accelerate your next-generation sequencing projects, cell-free DNA and genomic DNA analysis, RNA and proteins quality controls.
Does your lab need a Tech Upgrade? A technology upgrade may help you save time and money. During the workshop, you’ll hear the benefits your lab can get if you upgrade.
Come to the Workshop to:
<ul>
<li>Learn how the Advanced AE platforms perform in terms of hardware, software, and assays as well as workflows.</li>
<li>See equivalent data between the Bioanalyzer, TapeStation, and Fragment Analyzer system.</li>
<li>Discover the key advantages and benefits of the advanced platforms.</li>
<li>Listen to a customer experience.</li>
</ul>
<blockquote>
Presenter: Dr. Matthias Meister (Product Manager, Agilent Technologies Germany)
</blockquote>
<blockquote>
Matthias Meister is Product Manager at Agilent for the Bioanalyzer portfolio. He joined the marketing team for Automated Electrophoresis in 2022 and is based in Waldbronn, Germany. Prior to joining Agilent, he worked as a postdoctoral researcher at the German Cancer Research Center (DKFZ) in Heidelberg, where he also received his PhD.
</blockquote>
<blockquote>
Presenter: Sébastien Boulanger (Scientific Expert, Institute of Pathology and Genetics (IPG), Belgium)
</blockquote>
<blockquote>
Sébastien Boulanger integrated the Institute of Pathology and Genetics (IPG) of Gosselies as a lab technician in the molecular biology department in 1999. IPG is one of the eight Genetics Centers recognized in Belgium.
</blockquote>
<blockquote>
He gradually moved to a more scientific position and currently recognized by the Belgian society of Human Genetics as a Medical Genetics Laboratory Expert being now one of the main responsible of the technical lab development.
</blockquote>

Agilent: Upgrade your Tech with Agilent Advanced Automated Electrophoresis Solutions

Upgrade your Tech with Agilent Advanced Automated Electrophoresis Solutions

We want to help you gain the advantages from advanced automated electrophoresis solutions.

Agilent Advanced Automated Electrophoresis Solutions

Improving Process Performance
Event Program
<ul>
<li>The value of in-line measurement of quality-relevant analytical parameters</li>
<li>How a new pH sensor improves brewery processes</li>
<li>The importance of precise recognition of phase changes</li>
<li>How to ensure accuracy of dissolved oxygen sensors</li>
</ul>
Greater Confidence in Your Processes.
In-line measurement of quality-related analytics supplement process control with important information. 
In this webinar, we will show how the in-line measurement of relevant parameters helps to drive processes closer to their optimum.
One focus will be on the in-line measurement of pH and we will present a new sensor technology that simplifies the brewing process from mashing to filling. 
The webinar will also shed light on how the combination of color and turbidity measurement with scattered light at 25° and 90° has a positive influence on mashing, fermentation and filtration. And how transmitted light and reflection sensors optimize phase changes and enable product recognition.
Oxygen measurement along the process helps to detect faults in the system, enabling timely actions before out-of-spec oxygen causes lasting damage to your product.
The large number of measuring points in breweries requires a smart concept for the verification and calibration of in-line dissolved oxygen sensors. A solution for these tasks will also be presented.
<blockquote>
Presenter: Holger Schmidt (Mettler Toledo)
</blockquote>
<blockquote>
Holger Schmidt backs up his practical know how as brewer and malster with a brewmaster degree from TUM Weihenstephan. For two decades he has been globally mediating the triangle between Food and Beverage producers, plant and machine builders, and automation companies, with a focus on hygienic sensor technologies.
</blockquote>

Mettler-Toledo: In-line Quality Control in Breweries

In-line Quality Control in Breweries

In-line measurement of relevant parameters helps to drive processes closer to their optimum.

At the forefront of scientific inquiry lies the transformative potential of high-throughput screening in advancing drug discovery. Through the ingenious integration of cutting-edge technologies and automation, industry leaders are reshaping the landscape, enhancing efficiency and scaling up processes like never before.
In this exclusive extended webinar, immerse yourself in a world of innovation as our expert speakers unveil state-of-the-art systems and workflows designed to optimize screening assays with unparalleled throughput and reproducibility. Discover how these advancements propel drug discovery pipelines forward with unprecedented speed and precision.
Attend this webinar to:
<ul>
<li>Understand the benefits of integrated high-throughput screening automation in enhancing efficiency and scalability in drug discovery processes</li>
<li>Explore the features of a high-capacity workcell designed for complex compound screens with multiple analytical readouts</li>
<li>Learn about the advantages of a complete walkaway and tipless system in reducing downtime, minimizing waste and accelerating compound screening processes</li>
</ul>
<h3>Agenda</h3>
<h4>TALK 1</h4>
Next-Level Efficiency: Supercharged High-throughput Screening Capacity Through Integrated Automation
Discover how Automata’s cutting-edge platform streamlines drug discovery workflows, boasting a tipless system for seamless operation and enhanced scalability. Learn how the high-capacity workcell empowers researchers to conduct complex compound screens with unparalleled efficiency and reproducibility.
In this session, Alice Tone-Fernandez from Automata will valuable insights into leveraging integrated automation to supercharge high-throughput screening capacity, ultimately revolutionizing efficiency in drug discovery processes.
In her talk, she will:
<ul>
<li>Discuss the benefits of integrated high-throughput screening automation in enhancing efficiency and scalability in drug discovery processes</li>
<li>Explore the features of a high-capacity workcell designed for complex compound screens with multiple analytical readouts</li>
<li>Showcase the advantages of a complete walkaway and tipless system</li>
</ul>
<blockquote>
Presenter: Alice Tome-Fernandez (Senior Application Scientist, Automata)
</blockquote>
<h4>TALK 2</h4>
96 and 384 channel Electronic Pipettes from INTEGRA: Affordable, Simple, Small and Powerful
96 and 384 channel Electronic Pipettes from INTEGRA: Affordable, Simple, Small and Powerful
If you’re working in 24, 96, 384, or even 1536 plate formats, this session will show you 3 instruments which will make you faster, give you more reproducible results, are simple to learn and don’t take up much space - while fitting in every budget. The perfect productivity upgrade for any lab looking to get better.
In this session, Tom Bentivegna from INTEGRA will provide insights into best practices for utilizing these electronic pipettes effectively. Whether you’re a seasoned researcher or new to the field, this talk will provide valuable guidance for optimizing your laboratories workflows.
In his talk, he will:
<ul>
<li>Discuss how to optimize 96 and 384 well-plates to be faster and more reproducible</li>
<li>Showcase novel devices to enhance your high-throughput screening workflows</li>
</ul>
<blockquote>
Presenter: Tom Bentivegna (Group Product Manager, INTEGRA Biosciences)
</blockquote>
<h4>TALK 3</h4>
High-throughput SPR Assay for FcγR Binding to Drug Candidates on the New Carterra LSAXT
Discover the power of high-throughput surface plasmon resonance (HT-SPR) for assessing FcγR binding to drug candidates using the new Carterra LSAXT platform. In this webinar, Peter Hsueh, PhD from ACROBiosystems will showcase how this streamlined approach, coupled with ACROBiosystems’ recombinant FcγR proteins, enables rapid characterization of FcγR interactions across species.
In his talk, he will:
<ul>
<li>Explain the significance of FcγR binding in therapeutic efficacy and cross-reactivity assessment</li>
<li>Exploring a streamlined approach using HT-SPR on the Carterra LSAXT platform</li>
<li>Showcase the benefits of increased sensitivity, reproducibility and time/resource savings in characterizing FcγR interactions compared to traditional SPR methods</li>
</ul>
<blockquote>
Presenter: Peter Hsueh, PhD (Marketing Product Manager, ACROBiosystems)
</blockquote>

Technology Networks: Revolutionizing Drug Discovery: Unleashing the Power of High-Throughput Screening

Revolutionizing Drug Discovery: Unleashing the Power of High-Throughput Screening

Learn about state-of-the-art systems and workflows designed to optimize screening assays with unparalleled throughput and reproducibility.

The Power of High-Throughput Screening

There are numerous reasons why bringing the spectrophotometer to the sample instead of the tradition method of bring the sample to the spectrophotometer makes sense for making a measurement. During this presentation we will examine some of the reasons and look at aspects of using fiber optics on the Cary 60. Practical examples of samples will also be discussed.
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>

Agilent: The Art of Remote Sampling Using a Cary 60 and Fiber Optics

The Art of Remote Sampling Using a Cary 60 and Fiber Optics

During this presentation we will examine some of the reasons and look at aspects of using fiber optics on the Cary 60.

The Right Laboratory Balance Service at the Right Time
Event Program:
<ul>
<li>Selection of the right instrument for your application</li>
<li>Installation and qualification</li>
<li>Preventive maintenance</li>
<li>Calibration</li>
<li>Routine testing</li>
<li>Replacement</li>
</ul>
Weighing is a core process in almost every application in a lab. To maintain consistent results, it&#39;s essential to regularly service your equipment. But what are the most important services, and how often do they need to be performed?
In this webinar, we will guide you, based on the lifecycle of a balance, through the different services that can help you to keep your instrument accurate and extend its lifetime – from the instrument selection to its installation, throughout its routine operation, and up to the time for replacement.
Join our live webinar with METTLER TOLEDO experts to gain a better understanding.
<blockquote>
Presenter: Simona Venzin (Head of Service Product Management, METTLER TOLEDO)
</blockquote>
<blockquote>
Presenter: Walter Krebs (Head of GWP Product Management, METTLER TOLEDO)
</blockquote>

Mettler-Toledo: Service Balances the Smart Way

Service Balances the Smart Way

A guide through different services that can help you to keep your instrument accurate and extend its lifetime.

The science of elemental speciation is rapidly gaining traction, underscoring its critical role in safeguarding public health and ensuring regulatory compliance in food safety. Elemental speciation involves the precise identification and quantification of different chemical forms of elements within food substances, a practice that has profound implications for understanding the nutritional value, toxicity, and bioavailability of elements in our diet. As global food regulations evolve and consumer awareness heightens, the demand for rigorous and sophisticated analytical approaches in food testing has never been more acute.
This webinar is structured across two segments, each designed to provide an in-depth perspective on the multifaceted dimensions of food speciation analysis.
What You&#39;ll Learn:
<ul>
<li>An in-depth understanding of the current regulatory framework surrounding food speciation and its implications for the food industry.</li>
<li>The emerging challenges in food sample speciation and the comprehensive strategies developed to tackle these issues effectively.</li>
<li>A thorough exploration of Agilent&#39;s technological advancements, focusing on how HPLC and ICP-MS systems are being leveraged to set new standards in food safety analysis.</li>
<li>Insightful case studies highlighting real-world applications of advanced speciation techniques and their impact on ensuring food safety and regulatory compliance.</li>
</ul>
Who Should Attend:
<ul>
<li>Food Scientists and Technologists keen on updating their knowledge on the latest trends and technologies in food speciation.</li>
<li>Regulatory Affairs Specialists looking for a clearer understanding of current and upcoming regulatory requirements.</li>
<li>Quality Assurance and Quality Control Personnel aspiring to enhance their methodologies and compliance strategies in food safety.</li>
<li>Laboratory Managers and Analysts seeking to advance their technical skills and analytical capabilities in food testing.</li>
<li>Research Scientists and Academics aiming to deepen their research and teaching in the domain of food safety and analysis.</li>
</ul>
<blockquote>
Presenter: Ms. Jyoti Sindhu (Assistant Director - Lab and Business Unit Manager, Eurofins Analytical Services India Pvt. Ltd.)
</blockquote>
<blockquote>
Ms. Jyoti brings over 17 years of expertise in food safety, contaminants analysis, and quality assurance. Holding an M.Sc. in Food Technology, she has a robust background in elemental analysis, underpinning her commitment to ensuring food safety and managing contaminants through rigorous quality testing. Her skills extend beyond the laboratory, encompassing global client management, regulatory compliance, and a thorough understanding of ISO 17025:2017 standards. Currently, she is making significant contributions to the field as the Business Unit Head for Eurofins Analytical Services India, located in Bangalore, India.
</blockquote>
<blockquote>
Presenter: Dr. Vinay Jain (Food Workflows Commercialization Lead, Agilent Technologies International Pvt. Ltd.)
</blockquote>
<blockquote>
Dr. Vinay Jain is working as the CoE Manager and Country Food Workflow Commercialization Lead at Agilent Technologies International. He holds a Ph.D. in Analytical Chemistry and has more than 33 years of analytical and research experience in spectroscopic and mass spectrometry techniques. His specialization includes the development and validation of new analytical methods in accordance with regulatory guidelines. He has completed over 28 research projects with various national and international research and regulatory organizations. He holds two patents, has published 59 research papers, contributed two chapters to international handbooks, and authored 15 Agilent application notes. He received the Agilent TechInnovation Award in 2020 and 2022 for providing end-to-end workflow solutions. One of his innovations on LC-ICPMS was selected for the Winter Plasma Conference, and two were selected for the European Winter Plasma Conference for poster presentations.
</blockquote>

Agilent: Enhancing Food Safety through Advanced Speciation Analysis

Enhancing Food Safety through Advanced Speciation Analysis

This webinar provides an in-depth perspective on the multifaceted dimensions of food speciation analysis.

With the latest regulatory updates encompassing a pragmatic and structural related approach to determining the risk of N-nitrosamine impurities in pharmaceuticals, there remains the ongoing need for pharmaceutical manufacturers to consistently mitigate and control levels of these impurities in new and existing products. When required, analytical testing plays an important role in ensuring levels remain below regulatory acceptance thresholds.
In this webinar, Marian Twohig, Ph.D, Principal Scientist at Waters Corporation, will provide an overview of the more recent progress around N-nitrosamines regulations. Driven by the latest regulatory guidelines, Marian will share a comprehensive overview of the development of an analytical methodology for the quantification of N-nitroso propranolol in propranolol, citing unexpected challenges and the resolutions that lead to robust method optimization beyond regulatory required thresholds.
Key learning objectives:
<ul>
<li>Align analytical method optimization to current regulatory guidance.</li>
<li>The importance of high-quality chromatographic methods to facilitate ease of API and isomeric impurity resolution.</li>
<li>Accessible approaches in tandem quadrupole mass spectrometry solutions supporting the investigation and tentative identification of unexpected peaks and other method optimization challenges.</li>
</ul>
Who should attend?
Those working in pharmaceutical analytical development, pharmaceutical QA/QC, and regulatory in innovative and generic pharma, contract testing, and research and manufacturing organizations (CTO, CRO, CDMO).
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Marian Twohig (Principal Scientist, Waters Corporation)
</blockquote>
<blockquote>
Marian Twohig is a scientist with a passion for problem solving using analytical instrumentation. She has over 18 years of experience supporting quantitative and qualitative applications in the pharmaceutical, food and environmental and materials science industries. She works as a Principal Scientist for Waters Corporation where she has previously held several Senior Scientist positions.
</blockquote>

Waters Corporation: Managing the complexities of NDSRI method development: The N-nitroso propranolol case story

Managing the complexities of NDSRI method development: The N-nitroso propranolol case story

Overview of the development of an analytical methodology for the quantification of N-nitroso propranolol in propranolol.