What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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To help advance precision oncology, it is critical to identify the molecular changes that underlay tumorigenesis in solid tumors.
This webinar describes a new hybrid-capture target enrichment, next-generation sequencing (NGS) assay, run on the MGI DNBSEQ-G400 Sequencer and analyzed using SOPHiA GENETICS DDM™, that provides a modular and flexible approach to tumor characterization.
<blockquote>
Presenter: Felicity Hall, Ph.D. (NGS Product Manager, Agilent Technologies)
</blockquote>
<blockquote>
Felicity Hall is the Sr Global Product Manager for SureSelect Cancer Assays at Agilent Technologies. Felicity received her PhD in Molecular Biology from Monash University in Australia. Felicity has worked with market-leading global genomics companies and her expertise is diverse, spanning both Clinical Genomics and Life Science Genomics markets.
</blockquote>
<blockquote>
Presenter: Michael Ruvolo, Ph.D. (Director, R&amp;D, Agilent Technologies)
</blockquote>
<blockquote>
Mike is the director of NGS content development at Agilent Technologies. He and his team are responsible for assay development using SureSelect NGS target enrichment technology.
</blockquote>

Elevating Precision Oncology

New hybrid-capture target enrichment, next-generation sequencing (NGS) assay, run on the MGI DNBSEQ-G400 Sequencer and analyzed using SOPHiA GENETICS DDM™.

By attending this presentation, you will learn how to:
<ul>
<li>Improve PFAS analysis in biological samples with a simple yet effective novel sample prep technique</li>
<li>Achieve cleaner extracts for a wide range of PFAS compounds without compromising recovery</li>
<li>Implement an automated, robust, and reproducible sample prep method for PFAS analysis in biological samples with the Extrahera™ Sample Prep Workstation</li>
</ul>
Event Overview
PFAS analysis in biological samples can be quite challenging due to the complexity of sample matrices and the wide range of compound classes, each with different chemical characteristics. The ubiquitous nature of these compounds further complicates the sample preparation process.
In this webinar, Adam Senior from Biotage and Joe Homan from NMS Labs will present a novel sample prep method using the ISOLUTE® PLD+ for PFAS that overcomes some of these challenges. They will compare this method’s efficiency and effectiveness to other available techniques, illustrating how to achieve cleaner extracts for a wide range of PFAS compounds. Lastly, they will demonstrate how to automate sample preparation for PFAS analysis to achieve better reproducibility and enhance productivity.
What you need to know:
Date: 25 June, 2024
Start times:
Broadcast #1: 25 June - 7:30 am IST (New Delhi) / 10 am SGT (Singapore) / 10 am CST (Beijing) / 11 am JST (Tokyo) / 12 pm AEST (Melbourne) / 2 pm NZST (Auckland)
Broadcast #2: 25 June - 10 am BST (London) / 11 am CEST (Paris/Berlin) / 2:30 pm IST (New Delhi) / 5 pm SGT (Singapore)
Broadcast #3: 25 June - 10 am PDT (Los Angeles) / 12 pm CDT (Chicago) / 1 pm EDT (New York)
<blockquote>
Presenter: Adam Senior (Senior R&amp;D Analytical Scientist, Biotage)
</blockquote>
<blockquote>
Adam is an analytical chemist with more than 20 years of experience in method development in diverse applications. He is a Senior Scientist in the Research and Development group at Biotage. Adam is involved in the development and validation of LC-MS/MS sample preparation assays. His recent work has focused on the analysis of PFAS compounds in biological matrices, which led to the development of a new sample prep solution for this application.
</blockquote>
<blockquote>
Joseph Homan (Principal R&amp;D Scientist II, NMS Labs)
</blockquote>
<blockquote>
Joseph Homan is Principal R&amp;D Scientist II at NMS Labs in Horsham, Pennsylvania. He leads clinical and forensic analytical test development and validation. He explores, recommends, and implements scientific innovations, and optimizes current instrumentation and methodologies to improve quality, efficiency, profitability, and service.
</blockquote>

Separation Science: Overcoming Sample Prep Challenges with PFAS Analysis in Biological Fluids

Overcoming Sample Prep Challenges with PFAS Analysis in Biological Fluids

Discover a novel sample prep method to achieve cleaner extracts for a wide range of PFAS compounds.

Sample Prep for PFAS Analysis in Biological Fluids

Size, Polydispersity, Particle concentration and Zeta potential are crucial parameters for understanding the quality of LNP samples. The DynaPro ZetaStar instrument obtains all this critical quality attributes for you in mere seconds which makes it the perfect analytical equipment for evaluating LNP characteristics. In this webinar, we will showcase how the ZetaStar instrument can be utilized in Research and Development for better understanding how formulation, solvent conditions, storage, and stress can alter the properties of LNP.

Waters Corporation: Enhanced LNP quality assessment during Research and Development by DLS/SLS/ELS with the DynaPro ZetaStar Instrument

Enhanced LNP quality assessment during Research and Development by DLS/SLS/ELS with the DynaPro ZetaStar Instrument

LNP assessment by DLS/SLS/ELS with DynaPro ZetaStar

To advance research for precision oncology, it is essential to identify molecular changes that underly tumorigenesis.
Now you can tailor tumor genomic profiling assays by customizing next-generation sequencing (NGS) cancer panels to fit your laboratory’s specific requirements, including the assessment of new and emerging biomarkers, the immuno-oncology biomarkers (tumor mutational burden (TMB) and microsatellite instability (MSI)), and homologous recombination deficiency (HRD).
Agilent SureDesign, a user-friendly panel design software powered by machine learning, offers fast and easy design of Agilent SureSelect Cancer Custom panels to enable detection of key classes of somatic alterations. 
In this webinar, you will learn about:
<ul>
<li>The range of available tumor genomic profiling assays (comprehensive, tumor-specific, customized) in the Agilent SureSelect Cancer product portfolio</li>
<li>Designing customized cancer panels with SureDesign, by leveraging globally curated, cancer-specific genes and curating your own gene list</li>
<li>Achieving high-quality results from samples such as formalin-fixed, paraffin-embedded (FFPE) tissues </li>
<li>Improving lab efficiency by streamlining library preparation and target enrichment with automation, reducing hands-on time to as little as 15 minutes </li>
<li>Integrated workflows incorporating NGS sample and library QC, compatibility across multiple sequencing platforms, and informatics options</li>
</ul>
<blockquote>
Presenter: Danielle Fletcher (Field Applications Scientist, Agilent Technologies)
</blockquote>
<blockquote>
Danielle is a member of Agilent’s European custom design service. This team assists customers by creating custom NGS target enrichment panels as well as CGH and GE microarrays designs.
</blockquote>
<blockquote>
Presenter: Michael Ruvolo, Ph.D. (Director, R&amp;D, Agilent Technologies)
</blockquote>
<blockquote>
Mike is the director of NGS content development at Agilent Technologies.
</blockquote>
<blockquote>
He and his team are responsible for assay development using SureSelect NGS target enrichment technology.
</blockquote>

Agilent: Democratizing Tumor Genomic Profiling With Tailored Content

Democratizing Tumor Genomic Profiling With Tailored Content

Agilent SureDesign, a panel design software powered by machine learning, offers fast and easy design of Agilent SureSelect Cancer Custom panels.

Tumor Genomic Profiling With Tailored Content

Analyzing low-level analytes in complex biological matrices while maintaining superior sensitivity over the length of the study is a challenge for many researchers. Thus, triple quadrupole mass spectrometers are widely used in quantitative bioanalysis for their accuracy and precise measurement of pharmaceutical drugs in these complex matrices.
In this presentation, our speaker will introduce technology advancements that help ensure the highest levels of performance are maintained. He will share data that demonstrates how triple quadrupole mass spectrometers provide not only exceptional sensitivity but additional stability to support challenging bioanalytical workflows.
Attend this webinar to discover:
<ul>
<li>Technology innovations to support robust long-term bioanalysis</li>
<li>Best practices to achieve accurate and precise measurements by LC-MS in bioanalysis</li>
<li>How to preserve long-term method sensitivity in complex matrices</li>
</ul>
<blockquote>
Presenter: Rahul Baghla (Manager, Global Scientific Marketing, SCIEX)
</blockquote>

Technology Networks: Ensure Accuracy and Precision in Pharmaceutical Bioanalysis

Ensure Accuracy and Precision in Pharmaceutical Bioanalysis

Triple quadrupole mass spectrometers provide exceptional sensitivity and additional stability to support challenging bioanalytical workflows.

Accuracy and Precision in Pharmaceutical Bioanalysis

ZONTAL and Mestrelab by SciY are leaders in their respective areas of data digitalisation and complex analytical data processing. In this talk we explore how the two products can be seamlessly integrated to add convenience and value. Specifically, we look at how ZONTAL can harness the power of Mnova automated structure verification on data ingestion, and also how end users can use the familiar Mnova desktop application to directly search, access and save to the ZONTAL platform via Mnova Hub. These integrations enhance both products and enable them to be used with even greater efficiency when dealing with complex analytical data like NMR or LCMS.
Agenda
<ul>
<li>An introduction to Sciy</li>
<li>Getting value from scientific data – FAIR data</li>
<li>Getting value from scientific data – getting information and learning from complex data</li>
<li>Examples of seamless integration</li>
<li>Ingestion processing for automated structure elucidation</li>
<li>Ad hoc processing by users in a favoured desktop application</li>
</ul>
Key Learning Points
<ul>
<li>Why a FAIR data platform is important</li>
<li>How we can connect a FAIR data platform to end user applications to solve real laboratory problems</li>
</ul>
<blockquote>
Presenter: Dr. Christof Gaenzler (Director PreSales and Product Marketing)
</blockquote>
<blockquote>
Dr. Gaenzler holds a Doctorate in Molecular Biology from the University of Heidelberg and contributed to the development of the first HPV vaccines during his time at the German Cancer Center in Heidelberg. During his career outside of academia Dr. Gaenzler served as Senior Consultant and PreSales Manager at TIBCO Spotfire, and as the Senior Principal Product Marketing Manager for Biology at PerkinElmer Informatics. Currently, Dr. Gaenzler brings his expertise to ZONTAL as the Director of PreSales and Product Marketing.
</blockquote>
<blockquote>
Presenter: Gary Sharman, PhD (Senior Scientific Director)
</blockquote>
<blockquote>
Gary studied Natural sciences at the University of Cambridge before completing a PhD with prof Dudley Williams on structural and mechanistic studies by NMR and MS. He spent 20 years in the pharmaceutical industry, working in analytical chemistry at AstraZeneca and Eli Lilly, where he developed a particular interest in data flows and visualisations. He moved to Mestrelab in 2020, where his role centres around automation, data processing and customisation.
</blockquote>

Mestrelab Research: SciY Mestrelab Mnova & ZONTAL Join Forces for Next-Level Data Integration

SciY Mestrelab Mnova & ZONTAL Join Forces for Next-Level Data Integration

In this talk we explore how the two products can be seamlessly integrated to add convenience and value.

SciY Mestrelab Mnova & ZONTAL Data Integration

Together with ALS Czech Republic, we bring you an exclusive webinar series offering insights into the world of per- and polyfluoroalkyl substances (PFAS). The three-part series will bring together regulatory experts from REGARTIS and environmental laboratories professionals of ALS Czech Republic to address PFAS from environmental risks to the evolving regulatory landscape and analytical challenges.
First, we will explore their uses, environmental hazards, and restriction efforts. The second part will be focused on analytics, helping attendees understand PFAS testing in various matrices and the implications of their widespread environmental presence. Finally, the series will offer a discussion on regulatory processes, compliance strategies, and the technical aspects of PFAS analysis, such as sampling rules and possible matrices types. The core aim of the series is to provide chemical industry stakeholders with the knowledge to effectively manage PFAS compliance and analysis both.
<h3>Compliance and technical readiness</h3>
<ul>
<li>Ing. Jana Trávníčková, Ph.D.</li>
<li>Ing. Jana Kováčová, Ph.D.</li>
</ul>
In the final part of the series, co-hosted by REGARTIS and ALS Czech Republic, the focus will shift to the regulatory framework surrounding PFAS. This session will examine the background of PFAS restriction proposals, their reasonings, and the specific PFAS compounds involved.
Key topics will include the current status of PFAS restrictions and strategic preparation for forthcoming regulations, particularly regarding flame retardants. Additionally, ALS Czech Republic will introduce the technical aspects of PFAS analysis, including sample types and matrices, sampling rules, and logistical considerations when transporting samples for PFAS analyses. This part aims to equip attendees with the knowledge to navigate the evolving landscape of PFAS regulations and analytical methodologies.
<blockquote>
Presenter: Ing. Jana Trávníčková, Ph.D., (Regulatory Queen, Customer Happiness Manager – REGARTIS)
</blockquote>
<blockquote>
Jana studied chemistry at FCH VUT in Brno. She has been advising in the field of chemical European and national legislation since 2014. She can advise you on everything related to REACH, especially the authorization of SVHC substances, RoHS II, and the Regulation on Conflict Materials. She can also advise you on meeting the requirements of REACH in the area of safety data sheet management and CLP requirements.
</blockquote>
<blockquote>
Presenter: Ing. Jana Kováčová, Ph.D., (PFAS Section Supervisor – ALS Czech Republic)
</blockquote>
<blockquote>
Jana has been working in the field of analytical chemistry for more than 15 years. She defended her master&#39;s and doctoral studies at the University of Chemical Technology in Prague (UCT Prague), where her research focused on the development of analytical methods for determining pesticide residues in food and water. Jana joined ALS Czech Republic in the year 2020 as a validation specialist and focused her attention on optimising methods for the analysis of PFAS in environmental samples. At the beginning of 2022, she became the supervisor of the PFAS Section in Prague ALS Laboratories.
</blockquote>

ALS Czech Republic: PFAS: Strategic Approaches for Chemical Industry Compliance

PFAS: Strategic Approaches for Chemical Industry Compliance - Compliance and technical readiness

Examine the background of PFAS restriction proposals, their reasonings, and the specific PFAS compounds involved.

PFAS: Compliance and technical readiness

See how RapidFire is accelerating mass spec analyses for High Throughput Screening (HTS), ADME, biopharma, synthetic biology, food, and life sciences laboratories by replacing chromatography with a simple sample cleanup step and producing quality MS results in seconds.
We will discuss the latest developments in the RapidFire technology and how it enables a more productive, efficient, and cost-effective laboratory. Blazing-fast runtimes mean that your lab can eliminate backlog, improve turn-around times, and meet the demands of an ever-increasing number of samples. Improved sample consumption with the ability to use up to 7-fold less material translates to even more cost savings per sample. An increased plate storage capacity, support for 1536-wellplates, and temperature-controlled sample storage increase walk-away time, giving users more time to perform other lab duties. We will explore how labs are leveraging these advances, both individually and in tandem, as we discuss a variety of new applications demonstrating the benefits of this improved, fully integrated system.
Key Learnings:
<ul>
<li>See how to generate high quality mass spec data in seconds per sample</li>
<li>Learn strategies for improving lab efficency and productivity</li>
<li>Understand the advantages and limitations of Solid Phase Extraction Mass Spec</li>
</ul>
Who Should Attend:
<ul>
<li>Lab Managers</li>
<li>Lab Analysts</li>
<li>Chemist</li>
<li>Analytical Scientists </li>
<li>Lab Directors</li>
</ul>
<blockquote>
Presenter: Jennifer Hitchcock (Application Scientist, Agilent Technologies)
</blockquote>
<blockquote>
Jennifer is an Application Scientist with Agilent Technologies, developing methods utilizing LC and RapidFire front ends with mass spec detection for clinical and cannabis labs. Prior to joining Agilent in 2015, Jennifer worked as an R&amp;D scientist in a clinical testing lab in North Carolina. In her spare time, Jennifer enjoys spending time with her family in Richmond, Virginia.
</blockquote>
<blockquote>
Presenter: Kevin McCann (Product Manager, Agilent Technologies)
</blockquote>
<blockquote>
Kevin McCann focuses on high-throughput LC/MS solutions at Agilent Technologies in product marketing for RapidFire and StreamSelect. He has also worked as an application scientist, developing and supporting a variety of LC/MS applications.
</blockquote>
<blockquote>
Presenter: Christina Poggel (Product Manager, Wiley)
</blockquote>
<blockquote>
Christina Poggel has been leading the Science and Industry Content Strategy team at Wiley since 2024. Previously, she was Editor-in-Chief for Wiley Analytical Science from 2019, after joining Wiley as an Editor in 2017. Christina Poggel holds an M.Sc. in Chemistry and a Ph.D. in Organometallic Synthesis and Homogeneous Catalysis from Philipps University in Marburg, Germany. In the course of her research, she applied various spectroscopic, chromatographic, and mass spectrometric methods.
</blockquote>

Wiley: Feel the Need, the Need for Speed: Advances in RapidFire-MS Analyses for Faster, Larger Runs with Less Sample Preparation

Feel the Need, the Need for Speed: Advances in RapidFire-MS Analyses for Faster, Larger Runs with Less Sample Preparation

The latest developments in the RapidFire technology and how it enables a more productive, efficient, and cost-effective laboratory.

Advances in RapidFire-MS Analyses

Metastatic progression is the primary cause of death in most cancers, yet the regulatory dynamics driving the cellular changes necessary for metastasis remain poorly understood.
In this webinar, Dr. Steven Offer, Carver College of Medicine, University of Iowa Health Care, will discuss how a comprehensive multi-omics approach was used to identify the regulatory mechanisms in cancer metastasis.
By adopting this approach to study colorectal cancer invasiveness in a well-controlled experimental setting, the major regulators of this process were identified. Identifying these latent changes has the potential to greatly improve our understanding of the complex regulatory processes that control metastatic progression. This also helps to identify novel features of cancer that can be therapeutically targeted.
Key learning objectives
<ul>
<li>Understand how to identify and characterize epigenomic changes using various sequencing approaches (ChIP-seq, ATAC-seq) and mathematic models (pGENMi model)</li>
<li>Learn how to measure cell migration, invasion, and proliferation with label-free real-time cell analysis technology</li>
</ul>
Who should attend?
<ul>
<li>This webinar will provide insights for researchers who want to learn about multi-omics approaches to characterize epigenetic changes in cancer and metastasis.</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Steven Offer, Ph.D. (Associate Professor, Department of Pathology, Carver College of Medicine, University of Iowa Health Care)
</blockquote>
<blockquote>
Moderator: Dora Wells (Content Creator, SelectScience)
</blockquote>

SelectScience: Identifying Regulatory Mechanisms in Cancer Metastatic Processes with an Integrated Multi-Omics Approach

Identifying Regulatory Mechanisms in Cancer Metastatic Processes with an Integrated Multi-Omics Approach

Comprehensive multi-omics approach to identify the regulatory mechanisms in cancer metastasis.

Metastatic Processes with an Integrated Multi-Omics Approach

UV-Vis spectroscopy is a well-established technique in many laboratories. For decades, laboratories have adapted to pain points associated with UV-Vis workflows such as daily instrument warm-up, yearly lamp replacement, and slow and cumbersome temperature control systems.
Dr. Mathieu Rault, Cary 3500 Product Manager for Agilent Technologies, will present the Agilent Cary 3500 UV-Vis spectrophotometer series, developed from the ground-up to address these pain points and go beyond traditional user experiences. The Cary 3500 UV-Vis solution is a modular system that offers laboratories several benefits such as scalability, flexibility, and cost efficiency, as well as high analytical performances when measuring liquid and solid samples.
Each module has been designed and optimized depending on the sample type and analytical objectives. The modular design and software remove the burden of performing optical alignment procedures and the time and effort to recalibrate the instrument each time a module is changed. Swapping modules is quick, simple, and easy, allowing users to address specific application needs with tailored UV-Vis solutions.
Dr. Rault will also highlight UV-Vis applications for liquid and solid samples to illustrate the benefits of the instrument’s modular design.
Key learning objectives
<ul>
<li>Explore the benefits of using a modular UV-Vis spectrophotometer system in a laboratory setting to gain flexibility and increase cost efficiency</li>
<li>Understand how modular UV-Vis spectroscopy can benefit liquid sample analysis, such as offering simultaneous measurement of up to seven samples, small volume measurement, and characterization of solid samples.</li>
<li>Discover how the Cary 3500 smart modular design solution will help laboratories to achieve more, while reducing traditional UV-Vis spectroscopy pain points</li>
</ul>
Who should attend?
<ul>
<li>Pharma/biopharma QA/QC laboratory analysts and laboratory managers.</li>
<li>Chemical and energy laboratory analysists and laboratory managers.</li>
<li>Academia – PhD/ Post doctoral / Principal investigators.</li>
</ul>
Three sessions available
<ul>
<li>Thursday 27th June 2024 / 09:00 EDT / 14:00 BST / 15:00 CEST</li>
<li>Thursday 27th June 2024 / 12:00 PDT / 14:00 CDT / 15:00 EDT</li>
<li>Friday 28th June 2024 / 08:30 IST / 12:00 JST / 13:00 AEST</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Mathieu Rault (Global Cary 3500 Product Manager, Agilent Technologies, Australia)
</blockquote>
<blockquote>
Presenter: Carrie Haslam (Associate Editor, SelectScience)
</blockquote>

SelectScience: Modular UV-Vis Spectroscopy: A Smart and Scalable Approach to High-Performance Analysis

Modular UV-Vis Spectroscopy: A Smart and Scalable Approach to High-Performance Analysis

 Agilent Cary 3500 UV-Vis spectrophotometer series was developed from the ground-up to address UV-Vis pain points and go beyond traditional user experiences.