ISC 2026 - Day 2

<h4>What if you could compress 179 days of grueling laboratory workflow into just 14 hours?</h4>In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with analytical chemist Laura Penabad to discuss her groundbreaking new research in biocatalysis and high-throughput screening. Evolving engineered enzymes typically requires checking thousands of variants—a massive bottleneck when traditional Liquid Chromatography-Mass Spectrometry (LC-MS) runs take minutes per sample. Laura reveals how her team built a modular screening platform that shatters this limitation, running samples 128 times faster than standard methods without sacrificing analytical accuracy.Laura breaks down the "orchestra" of technologies making this possible, including coupling nanoliter droplet microfluidics with Cyclic Ion Mobility-Mass Spectrometry (cIM-MS) to separate complex chemical isomers in milliseconds. She also shares the chaotic but triumphant behind-the-scenes stories of her PhD journey—from presenting a "comically bad" early poster at ASMS to recruiting an undergraduate mechanical engineer to build custom automation software from scratch.If you are an early-career researcher, a mass spec enthusiast, or curious about the future of synthetic biology and machine learning in chemistry, this episode is packed with invaluable intuition and advice.<h3>Video Transcription</h3><h4>From Months to Hours: Droplet Ion Mobility–MS for Biocatalysis Screening</h4>High-throughput screening is central to directed enzyme evolution, but conventional analytical workflows can create a difficult compromise: extremely fast screens often provide limited structural information, while techniques such as LC-MS offer much richer chemical insight at the cost of throughput. In a recent interview, analytical chemist Laura described how her work with droplet-based cyclic ion mobility mass spectrometry is helping bridge that gap—providing rapid analysis while retaining the ability to distinguish structurally similar products.Her research grew from an initially modest proof of concept into a screening platform capable of analyzing a 96-well plate in roughly ten minutes. The resulting workflow was approximately 128 times faster than a conventional three-minute LC-MS analysis, opening new possibilities for directed evolution campaigns in which thousands of enzyme variants need to be evaluated.<h4>Directed evolution needs better analytical screening</h4>Biocatalysis takes advantage of enzymes as highly selective catalysts capable of performing reactions under relatively mild conditions. Directed evolution extends those capabilities by deliberately modifying enzyme sequences, expressing large numbers of variants, screening their activity, and using the best-performing mutants as starting points for subsequent rounds of evolution.The process can be repeated multiple times, progressively steering an enzyme toward improved activity, altered substrate specificity, or even chemistry that does not occur naturally.But the effectiveness of directed evolution depends heavily on the screening step. The more variants that can be analyzed—and the more useful chemical information that can be collected for each one—the more effectively researchers can explore the enormous sequence space available to an enzyme.For Laura, this created a clear analytical challenge: develop a screening platform that was substantially faster than conventional chromatographic analysis without sacrificing the structural discrimination needed for chemically similar products.<h4>From an imperfect first experiment to a working platform</h4>The project did not begin with a polished high-throughput workflow.Laura recalled looking back at her 2023 ASMS poster, where the early system was still working with standards rather than true isomeric samples. Droplet introduction was slow—around six seconds per droplet—and the signals were far from the ideal reproducible, square, flat-topped profiles expected from replicate droplets.Nevertheless, simply producing a droplet signal represented an important first milestone.The decisive change came when the project stopped being treated merely as a demonstration that droplet ion mobility–MS could work and instead became a deliberate effort to build a robust screening platform.Laura approached the problem in modules. First came reliable droplet introduction. Then the ion mobility separation method. Quantification became another independent component. Matrix effects were introduced later, gradually making the system resemble the conditions expected with real screening samples.Each layer was tested before another was added.This stepwise strategy became one of the central principles of the project: rather than attempting to optimize the entire system simultaneously, individual components were developed, challenged, and then assembled into an increasingly realistic workflow.<h4>Finding the middle ground between speed and structural information</h4>One of the biggest analytical problems in high-throughput screening is distinguishing products with the same nominal or exact mass.Constitutional isomers may contain the same atoms and therefore generate the same mass-to-charge ratio, while differing only in how functional groups are arranged within the molecular structure. Very rapid optical or mass-based screens may therefore struggle to distinguish them.Traditional LC-MS can solve many of these problems through chromatographic separation, but a several-minute analysis rapidly becomes impractical when thousands of enzyme variants must be screened.Ion mobility offered Laura’s team another option.Rather than separating compounds on a chromatographic timescale, ion mobility can differentiate ions according to their gas-phase behavior on a much shorter timescale. In the workflow described in the interview, this created a useful compromise: not the kilohertz throughput possible with some simple screening technologies, but dramatically faster structural differentiation than conventional LC-MS.The goal was therefore not simply to achieve the fastest possible screen, but to find a middle-throughput analytical regime with enough resolving power to answer chemically meaningful questions.<h4>A 96-well plate in about ten minutes</h4>That strategy eventually produced one of the most striking results of the project.The droplet cyclic ion mobility–MS platform processed a 96-well plate in roughly ten minutes, while maintaining quantitative results comparable with the conventional LC-MS workflow used for comparison. According to the interview, this represented an approximately 128-fold increase in analysis speed relative to a three-minute LC-MS run.Laura described the successful plate experiment less as a surprise than as confirmation that the modular development strategy had worked.Before reaching that point, the workflow had already been challenged through a sequence of increasingly demanding experiments: droplet formation, ion mobility separation, calibration, matrix-containing calibration, and cross-testing analytes at different concentrations. By the time the complete plate was analyzed, each individual part of the analytical system had already been tested under progressively more realistic conditions.<h4>What does that speed mean for directed evolution?</h4>The impact becomes even clearer when the workflow is considered at the scale of an entire directed-evolution campaign.Laura’s manuscript estimated that a five-round campaign involving 5,000 variants would require approximately 179 days using the conventional LC-MS workflow but only about 14 hours using the new platform.Compressing months of analytical work into less than a day has consequences beyond laboratory productivity.It allows researchers to screen more enzyme variants and therefore sample a larger portion of sequence space. But the resulting datasets may also become increasingly valuable for computational approaches.Rather than retaining information only about the best-performing enzyme variant, researchers can preserve data describing variants that performed poorly as well as those that succeeded. When genotype and phenotype information remain linked through the well-plate workflow, these larger datasets can potentially provide richer input for machine-learning models designed to guide subsequent rounds of enzyme evolution.Faster analysis therefore does more than accelerate an existing experiment—it can change the scale and design of the experiment itself.<h4>Balancing ion mobility resolution and signal</h4>Increasing cyclic ion mobility separation time can improve resolution, but it also introduces an important trade-off: additional passes can reduce signal.Laura optimized this balance experimentally by continuously infusing mixtures containing both isomers and progressively adjusting the ion mobility conditions. After tuning parameters such as wave height and velocity, increasing the separation to three passes provided baseline resolution at the high analyte concentrations used during method development.<blockquote>Concentration, however, also mattered.</blockquote>At higher ion populations, space-charge effects could broaden peaks and reduce the baseline separation. This meant that the optimal number of passes could not be selected independently of the concentration range required by the final assay.Her broader advice for optimization is therefore application-driven: decide first what the analytical method actually needs to accomplish.More speed will almost always require sacrificing something—resolution, signal intensity, quantitative precision, or another performance parameter. The key is to identify which compromise the application can tolerate and then optimize the system around that requirement.<h4>Solving droplet carryover with a practical compromise</h4>Droplet-based workflows present their own engineering challenges, including carryover between neighboring samples.In Laura’s system, analytes showed little tendency to partition into the fluorinated oil separating aqueous droplets within the Teflon tubing. The more troublesome carryover occurred closer to electrospray ionization, where a small amount of sample could remain associated with the interface and appear when the next droplet arrived.Rather than adding excessive engineering complexity to an already elaborate platform, the researchers adopted a pragmatic solution: a sacrificial wash droplet between analytical samples.It is an example of a recurring theme throughout the project. The objective was not to construct the theoretically most sophisticated droplet system, but to create a screening workflow that remained sufficiently robust, reproducible, and practical for large numbers of samples.<h4>High-throughput analysis also requires high-throughput data processing</h4>Accelerating the mass spectrometry experiment created another bottleneck: the data.The instrument produced a continuous intensity-versus-time trace rather than a neatly organized well-plate dataset. With small experiments, Laura could manually identify individual droplets and process the results in spreadsheets. That approach quickly became unrealistic as sample numbers increased.The solution emerged through collaboration with an undergraduate mentee, Aiden, who helped develop a dedicated software tool for processing the continuous traces.Laura defined how the software should behave and how the analytical information should be organized, while Aiden developed the computational implementation. The workflow itself was subsequently adjusted to make automated data processing more reliable—for example, by introducing marker columns that allowed the software to recognize the end of each plate row.The resulting tool can accept the acquired traces together with information about plate dimensions and replicates and automatically return parameters including peak identity, duration, and the corresponding well position. A later version also generated an automatically populated heat map.The experience illustrates how improving analytical throughput often requires simultaneous development of instrumentation, experimental design, and informatics. Making the measurement faster is useful only if the resulting data can be processed at a comparable speed.<h4>Mentoring became part of the scientific process</h4>The software project also reflects another theme Laura emphasized throughout the interview: the value of mentoring.Several younger researchers contributed directly to the development of the final platform. Laura described mentees not simply as additional hands in the laboratory but as scientists capable of proposing their own hypotheses and experimental ideas.Working with undergraduate researchers also influenced the way she organized experiments.Because their available laboratory time was limited, each task needed to have a clear purpose. That encouraged a more disciplined approach to experimental planning: instead of performing experiments simply because they could be done, the team had to ask which experiment would most efficiently answer the next important question.In that sense, mentoring became another mechanism for improving the research itself.<h4>The next step: continuous, automated screening</h4>Despite the dramatic increase in analytical speed, the current workflow still includes manual operations such as loading tubing and connecting the sample stream to the mass spectrometer.The next engineering challenge is therefore automation.Laura described ongoing work using the Venturi effect to pull samples directly from wells into the droplet system. Building on previous work in the laboratory with 96-well plates, she has already tested this approach with as many as 2,000 samples.The long-term direction is clear: continuous and robust droplet generation, automated handling of thousands of samples, rapid ion mobility separation, mass spectrometric detection, and automated reconstruction of the resulting data into plate-based outputs.Combining those elements could move the system closer to the type of large-scale screening required for increasingly ambitious biocatalysis campaigns.<h4>Learning mass spectrometry one problem at a time</h4>Laura also reflected on the broader experience of developing the platform during her PhD.Ion mobility and mass spectrometry initially appeared intimidating, particularly because they combine concepts from analytical chemistry, physical chemistry, separations, instrumentation, and electrical engineering. What eventually changed was not simply learning more facts, but developing the ability to move repeatedly between theory and experiment.An unexpected result sends the researcher back to the underlying theory. A better understanding suggests a modified experiment. That experiment creates another observation, gradually building intuition about how the system behaves.Her advice to early-career scientists is therefore to accept that this process takes time.Advanced analytical techniques may initially appear overwhelming, but understanding develops incrementally through repeated interaction between theory, experimentation, failure, and refinement. Equally important, she emphasized the role of the supportive ion mobility–mass spectrometry community in making a technically demanding field more approachable for young researchers.From the first inconsistent droplets to a platform capable of processing thousands of samples, that iterative mindset ultimately shaped the entire project.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Droplet Cyclic Ion Mobility Mass Spec: Compressing Months of Enzyme Screening Into Just 14 Hours

ISC 2026 - Day 3

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The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Scientific Orbitrap Exploris 480 Mass Spectrometer

Empowering life science researchers to translate each experimental step to a new level of insight with built-in workflows and single-cell sensitivity.

Empowering life science researchers to translate each experimental step to a new level of insight with built-in workflows and single-cell sensitivity. Accelerate your path to impactful results with a next-generation ownership experience coupled with an intelligence-driven experimental approach. Achieve a new standard in performance to study trace samples with extraordinary accuracy, certainty, confidence and simplicity. You'll find market leading quantitative performance to solve your most complex challenges with ease, and the proven qualitative performance you expect from Orbitrap- based mass spectrometry.From exploratory to targeted quantitation, find the power and reliability to make large scale studies possible, while reducing everyday hassles as you increase productivity. And achieving it all, remarkably, within a compact footprint. An extraordinary instrument, providing extraordinary results; the Thermo Scientific Orbitrap Exploris 480 mass spectrometer.<h4>Key benefits of the Orbitrap Exploris 480 mass spectrometer</h4><h5>Next-generation experience</h5>Built with next-generation common hardware and software architecture, the guiding principle of ultimate performance and ease of use delivers effortless access to the value contained within your data.<h5>High performance, high-throughput insights</h5>Adding greater performance and intelligence to market-leading selectivity and spectral quality imparts productivity, depth, and certainty to small and large- scale studies.<h5>Robust performance and exceptional serviceability increase uptime</h5>By design, exceptional end-to-end robustness minimizes need for performance-related maintenance, increasing uptime and sample throughput.<h5>Ease-of-use and intelligence to empower all users</h5>Differences in user expertise can be a challenge in many laboratories. Workflows based on intelligent data acquisition methods leverage new levels of instrument performance to deliver high confidence, and high throughput.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Ion_path_and_key_components_of_the_Orbitrap_Exploris_480_mass_spectrometer_3f5791378c.webp" alt="Thermo Fisher Scientific: Ion path and key components of the Orbitrap Exploris 480 mass spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Ion_path_and_key_components_of_the_Orbitrap_Exploris_480_mass_spectrometer_3f5791378c.webp 245w," sizes="100vw" width="950" height="410"><div><h4>How does the Orbitrap Exploris 480 MS enable greater data insights faster?</h4></div><div>See what makes the Orbitrap Exploris 480 MS stand out, how it can empower all users to obtain the highest quality results and deepest insights with the least amount of effort; see how our breakthrough is now your breakthrough.</div><ul><li>Next-Generation Instrument Design</li><li>Targeted Protein Quantitation Workflows with SureQuant IS</li><li>Advancing Accuracy, and Precision in Quantitative Proteomics through Multiplexing</li><li>Sub 1-ppm Mass Accuracy as Standard</li><li>Improved Performance through Instrument Enhancements</li><li>Intelligence-Driven Instrument Set-Up, Control and Data Acquisition</li><li>Visualize the Orbitrap Exploris Data Acquisition Modes</li></ul><h4>Optional features to maximize performance</h4>Maximize performance and flexibility to expand experimental capabilities of the Orbitrap Exploris 480 mass spectrometer with multiple hardware and software options, allowing you to see more, quantify more, deliver more confidence and greater insights with even more productivity.<ul><li>Enhance your Experimental Performance with the FAIMS Pro Duo interface</li><li>Comprehensive and confident biotherapeutic characterization</li></ul><div data-uw-styling-context="true"><h4>Data analysis made easy</h4></div><div data-uw-styling-context="true">From complex proteomic challenges, quantitation with SureQuant and TMT multiplexing, through to complete biotherapeutic characterization, data analysis doesn’t have to be the bottleneck to accessing deeper insights into your high-quality MS data. Despite the numerous workflows involved in the analysis of proteins, there are flexible analysis tools which allow simple, yet confident, interpretation of your data to streamline your research and facilitate decision making to take your research to the next level.</div><ul><li>Flexibly Identify and Quantify Proteins Based on your Workflow</li><li>The Right Path Towards Complete Biotherapeutic Characterization</li><li>Compliance and Enterprise Readiness with Chromeleon CDS Software &nbsp;</li></ul>

Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer

Orbitrap Fusion Tribrid MS combines quadrupole, ion trap, and Orbitrap analyzers for ultra-flexible MSⁿ workflows, enabling deep proteomics, PTM analysis, and confident structural elucidation.

<h4>Revolutionary tribrid architecture for comprehensive structural analysis and deep proteome coverage</h4>The Orbitrap Fusion Tribrid mass spectrometer combines a quadrupole, a linear ion trap, and an Orbitrap mass analyzer in a unique architecture that delivers unmatched analytical performance. It provides life scientists with advanced MSn capabilities, flexibility in fragmentation techniques, and exceptional resolution and speed — ideal for tackling the most complex analytical challenges in proteomics, PTMs, metabolomics, lipidomics, and small molecule research.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_e93c577ae5/Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_e93c577ae5/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr.webp 245w," sizes="100vw" width="1914" height="1059"><h3>Key Features and Benefits</h3><h4>🔬 Revolutionary Tribrid Design</h4><ul><li>Combines quadrupole, linear ion trap, and Orbitrap analyzers</li><li>Perform MSⁿ up to n = 10 with detection in either ion trap or Orbitrap</li><li>Fragmentation options: CID, HCD, ETD, and EThcD at any MS stage</li><li>Synchronous Precursor Selection (SPS): up to 20 precursors per scan for MS³</li></ul><h4>⚙️ High Performance &amp; Resolution</h4><ul><li>Resolving power up to 500,000 FWHM at m/z 200</li><li>&lt;1 ppm mass accuracy with EASY-IC™ internal calibration</li><li>Polarity switching in 1.1 s full cycle</li><li>Scan speeds up to 20 Hz for Orbitrap and ion trap MSn</li></ul><h4>🧪 Versatility in Applications</h4><ul><li>Analyze low-abundance, high-complexity, or structurally complex samples</li><li>Confidently resolve isobaric interferences and detect PTMs</li><li>Full compatibility with intact protein analysis, glycoproteomics, and TMT SPS-MS³ quantitation workflows</li></ul><h4>🔁 Efficient Ion Handling</h4><ul><li>EASY-Max NG or NanoSpray Flex NG ion sources</li><li>Active Beam Guide and S-Lens for efficient ion transmission</li><li>Dynamic Scan Management optimizes scan scheduling and parallelization</li></ul><h4>💡 Ease of Use</h4><ul><li>Intuitive Tune and Method Editors</li><li>Method templates for routine and advanced workflows</li><li>Quanfirmation: HRAM SIM for quantitation + ion trap MS/MS for confirmation</li><li>Automated calibration, AGC, and exclusion logic</li></ul><h4>🧬 Compatible Software</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Xcalibur™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Proteome Discoverer™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1756">ProSight™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757">Mass Frontier™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Compound Discoverer™</a>, and others</li><li>Support for data-dependent, targeted, and deconvolution workflows</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_2_3d7accb56e/Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr-2.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Tribrid_hmotnostni_spektrometr_2_3d7accb56e/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Tribrid-hmotnostni-spektrometr-2.webp 245w," sizes="100vw" width="1038" height="622">

Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer

The Orbitrap Fusion Lumos Tribrid MS combines quadrupole, ion trap, and Orbitrap analyzers for high-resolution, ultra-sensitive MSn workflows in proteomics, metabolomics, and biopharma.

<h4>The most powerful tribrid MS platform for deep, multidimensional analysis in proteomics, metabolomics, and structural biology</h4>The Orbitrap Fusion Lumos Tribrid MS is a cutting-edge mass spectrometer that combines a quadrupole, a linear ion trap, and an Orbitrap™ high-resolution accurate-mass analyzer in a single platform. With unmatched sensitivity, selectivity, and versatility, it enables scientists to tackle complex biological questions with confidence—whether in discovery proteomics, targeted quantitation, top-down protein analysis, glycoproteomics, lipidomics, or small molecule research.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_1_39be5478b4/Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-1.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_1_39be5478b4/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-1.webp 245w," sizes="100vw" width="1823" height="1068"><h3>🔬 Unmatched Analytical Performance</h3><ul><li>&gt;500,000 resolution with Orbitrap Ultra-High Field analyzer</li><li>Sub-ppm mass accuracy with real-time calibration</li><li>Attomole-level quantification using PRM and DIA</li><li>High sensitivity MS/MS with HCD, CID, ETD, EThcD, and new ETD HD fragmentation</li></ul><h3>🔄 Flexibility Across Workflows</h3><ul><li>Tribrid architecture: combine quadrupole precursor selection, ion trap MSn, and Orbitrap detection</li><li>DIA, PRM, and SPS-MS3 quantification workflows with isobaric tags (e.g., TMT, TMTpro)</li><li>Top-down intact protein analysis with high sequence coverage</li><li>Fast polarity switching and intelligent acquisition for lipidomics</li></ul><h3>🧪 Ideal for Multi-Omics and PTMs</h3><ul><li>Deep profiling of glycopeptides, phosphoproteins, and other post-translational modifications</li><li>Resolution of isobaric isoforms, methylation patterns, and histone modifications</li><li>Confident structure elucidation of small molecules with fine isotope resolution</li></ul><h3>💡 Smart Technology for Productivity</h3><ul><li>Advanced Quadrupole Technology (AQT) for high transmission and selectivity</li><li>Automatic gain control (AGC) for optimal ion population</li><li>Easy ETD™ source and vent-free maintenance with source plug</li><li>Method templates and visual method editor for intuitive setup</li></ul><h3>🧬 Applications</h3><ul><li>Quantitative and discovery proteomics (e.g., large-scale TMT SPS-MS3)</li><li>Structural characterization of antibodies and proteoforms</li><li>Targeted metabolite quantitation and small molecule identification</li><li>Comprehensive glycoproteomics and lipidomics workflows</li></ul><h3>🧷 Software Compatibility</h3><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1758">Xcalibur™</a> and Tune for control and calibration</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1782">Proteome Discoverer™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1756">ProSight™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/77">TraceFinder™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1757">Mass Frontier™</a>, <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1759">Compound Discoverer™</a> for data analysis across omics</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_3_7458a4eb26/Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-3.webp" alt="Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Pragolab_Thermo_Orbitrap_Fusion_Lumos_Tribrid_hmotnostni_spektrometr_3_7458a4eb26/thumbnail_Pragolab-Thermo-Orbitrap-Fusion-Lumos-Tribrid-hmotnostni-spektrometr-3.webp 245w," sizes="100vw" width="2578" height="1296">

Products focused on LC/MS - page 3

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ISC 2026 - Day 3

Droplet Cyclic Ion Mobility Mass Spec: Compressing Months of Enzyme Screening Into Just 14 Hours

ISC 2026 - Day 2