<h3><strong>We are pleased to inform you about the enhanced control capabilities offered through the <a href="https://lcms.labrulez.com/products?manufacturerItems=95&amp;search=Clarity">Clarity software family</a>.</strong></h3>
<h3><strong>Clarity - Shimadzu Edition</strong></h3>
<p>Clarity - Shimadzu Edition expands instrument control options of Clarity Chromatography Software with <a href="https://lcms.labrulez.com/library?instrumentationItems=27&amp;mainauthorItems=1&amp;search=Nexera"><strong>Shimadzu Nexera HPLC</strong></a> and <a href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=1&amp;search=Nexis"><strong>Nexis GC systems</strong></a>.</p>
<p>In addition to all supported instruments in Clarity, this edition brings exclusive control of Shimadzu Nexera HPLC and Nexis GC systems. </p>
<p><em>The control of Shimadzu Nexis GC-2030 GC and Shimadzu Nexera HPLC is available only through a special Clarity OEM version called Clarity - Shimadzu Edition (p/n C50-28) which is available only in Europe.</em></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_Shimadzu_Edition_13a618e618/DataApex-Clarity-Shimadzu-Edition.jpg" alt="DataApex: Clarity - Shimadzu Edition"/></p>
<h4><strong>All Clarity features in the Shimadzu edition</strong></h4>
<p>You will get all the optional features available in Clarity with your Shimadzu edition, such as integrated control of selected instruments (GCs, LCs, pumps, detectors, autosamplers, etc.). Extensions provide functions for specific separation techniques or calculations such as <a href="https://lcms.labrulez.com/products/1001"><strong>PDA</strong></a>, <a href="https://lcms.labrulez.com/products/1004"><strong>GPC analysis</strong></a>, <a href="https://lcms.labrulez.com/products/1003"><strong>Mass spectrometry</strong></a>, or <a href="https://lcms.labrulez.com/products/1002"><strong>SST</strong></a>. User interface choice of languages includes English, French, German, Russian, Spanish, and Chinese. Our highly rated technicians and developers provide timely, extensive support.</p>
<h3><strong>Control module for <a href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=1&amp;search=Nexis+2030">Shimadzu Nexis GC-2030</a> system</strong></h3>
<p>The Shimadzu Nexis GC-2030 Control module allows direct control of this GC (Gas chromatograph) using Agilent ICF (Instrument Control Framework). Direct control means that the GC can be completely controlled from the Clarity - Shimadzu Edition Chromatography Station enabling integrated GC control thus ensuring complete automation for laboratories. The instrument method controlling the analysis conditions will be saved in measured chromatograms. Along with the above-mentioned CG, the control module also allows controlling autosamplers operated through the GC. The control is performed via LAN. The minimum required firmware of the GC is version 1.09.</p>
<h4><strong>Specification</strong></h4>
<p><strong>Shimadzu Nexis GC-2030</strong></p>
<ul>
<li>Controlled instruments: Nexis GC-2030 gas chromatograph, AOC-20 family autosamplers</li>
<li>Supported detectors: FID, TCD, FTD, FPD, ECD, BID</li>
<li>Communication interface: LAN</li>
<li>Purchasing: GC Control - (p/n A23), GC Shimadzu Control (p/n A23-001) - needs to be purchased once for each connected system, AS Control - (p/n A26) - when autosampler is present</li>
<li>Related products: Clarity - Shimadzu Edition (p/n C50-28) Only available in Europe</li>
<li>Cable: Cross LAN (p/n SK08)</li>
</ul>
<h3><strong>Control module for <a href="https://lcms.labrulez.com/library?instrumentationItems=27&amp;search=Shimadzu+Nexera+HPLC">Shimadzu Nexera HPLC</a> systems</strong></h3>
<p>Direct control of Shimadzu Nexera HPLC components using the Agilent ICF (Instrument Control Framework) tools developed by the Agilent company. The Clarity Chromatography Station - Shimadzu Edition allows for direct control of selected Shimadzu Nexera components (mostly from the Nexera LC-40 line), enabling Clarity to provide integrated instrument control and to ensure complete automation for laboratories. The control module includes parameters for the HPLC pumps, column ovens, detectors (including PDA), autosamplers, etc. A dedicated PDA Extension enables the evaluation of data from PDA systems.</p>
<p>The control module limits the usage of multiple Nexera HPLC systems in the same Clarity - only a single system is guaranteed to function reliably.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Shimadzu_Nexis_GC_2030_Control_10da3b4ef0/DataApex-Shimadzu-Nexis-GC-2030-Control.jpg" alt="DataApex: Shimadzu Nexis GC-2030 Control"/></p>
<h4><strong>Specification</strong></h4>
<p><strong>Shimadzu Nexera Control</strong></p>
<ul>
<li>Controlled devices: The Shimadzu Nexera HPLC control module is compatible with selected components of Shimadzu Nexera HPLC systems supported by the Agilent ICF. Exact list of controlled components is shown in the list below.</li>
<li>Communication interface: LAN</li>
<li>Data acquisition interface: Digital Data Acquisition<br/>Purchasing: LC Control (p/n A24), LC Shimadzu Control (p/n A24-001) - needs to be purchased once for each connected system, AS Control (p/n A26) - optional, PDA Extension (p/n A29) - optional</li>
<li>Special: Based on controller serial number and unique LC Shimadzu Control license number, specific Connect Key is needed for the LC to communicate, for more details see the manual.</li>
<li>Related products: Clarity - Shimadzu Edition (p/n C50-28)</li>
<li>Cable: Cross LAN (p/n SK08)</li>
</ul>
<p><strong>List of controlled subdevices of the Shimadzu Nexera control module. Please note that the portfolio of components widens with further releases of newer control module versions.</strong></p>
<ul>
<li>Controllers: CBM-40, CBM-40 Lite, SCL-40</li>
<li>Pumps: LC-40B X3, LC-40B XR, LC-40D, LC-40D X3, LC-40D XR, LC-40D XS</li>
<li>Detectors: CDD-10Avp, RF-20A, RF-20AXS, RID-20A, SPD-40, SPD-40V</li>
<li>PDA Detectors: SPD-M30A, SPD-M40</li>
<li>Autosamplers: SIL-40, SIL-40 XR, SIL-40C, SIL-40C X3, SIL-40C XR, SIL-40C XS</li>
<li>Column Ovens: CTO-30A, CTO-40C, CTO-40S</li>
<li>Other: Mobile Phase Monitor MPM-40</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Shimadzu_Nexera_Control_1a5d578355/DataApex-Shimadzu-Nexera-Control.jpg" alt="DataApex: Shimadzu Nexera Control"/></p>
<p><a href="https://www.dataapex.com/controls"><strong>List of all supported instruments</strong></a></p>
<blockquote>
<p><strong><a href="https://www.dataapex.com/product/clarity-shimadzu-edition-demo">Demo version available</a></strong></p>
</blockquote>
<blockquote>
<p>Preview functions of Clarity - Shimadzu Edition. You can try any controlled instrument in simulated demo mode without connecting to a real device. Discover all configuration options and run simulated data acquisition. A stored demo data set allows you to try the capabilities of data processing.</p>
</blockquote>


Nexera HPLC and Nexis GC systems  Supported in Clarity

<p><strong>In the next 14 days, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p>
<blockquote>
<h4><a href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Agilent Technologies: Evolving Landscape on Control of Excipients Quality in Pharmaceutical Products</strong></h5>
<ul>
<li>Th, 22.2.2024, 12:00 CET</li>
<li><a href="https://gcms.labrulez.com/webinar/3535">Registration</a></li>
</ul>
<p>Join this webinar to hear IPC, USP and Agilent experts share their views on the role of Pharmacopeia&#39;s in ensuring Quality of Pharmaceutical products and excipients.</p>
<h5><strong>2. Wiley: Introduction to KnowItAll for IR &amp; Raman (Vibrational Spectroscopy)</strong></h5>
<ul>
<li>Th, 22.2.2024, 16:00 CET</li>
<li><a href="https://gcms.labrulez.com/webinar/3570">Registration</a></li>
</ul>
<p>We will demonstrate how KnowItAll can help solve unknown IR and Raman spectra through a series of examples.</p>
<h5><strong>3. Agilent Technologies: See Through the Barrier: The Power of Agilent&#39;s Solutions for Raw Material Verification</strong></h5>
<ul>
<li>Fr, 23.2.2024, 19:00 CET</li>
<li><a href="https://gcms.labrulez.com/webinar/3354">Registration</a></li>
</ul>
<p>This talk will delve into the prowess of these Agilent Spatially Offset Raman spectrometers, emphasizing their capability to ensure material authenticity even through obstructions.</p>
<h5><strong>4. Agilent Technologies: Harmonized USP Chapter 621 Chromatography – Recent Revisions</strong></h5>
<ul>
<li>Tu, 27.2.2024, 07:00 CET</li>
<li><a href="https://gcms.labrulez.com/webinar/3573">Registration</a></li>
</ul>
<p>In this webinar we will give an overview of General Chapter &lt;621&gt; Chromatography and the allowed adjustments to chromatographic conditions in the USP monographs. </p>
<h5><strong>5. Agilent Technologies: Navigating Troubled Waters: A Deep Dive into Water Injections by GC</strong></h5>
<ul>
<li>Tu, 27.2.2024, 17:00 (CEST)</li>
<li><a href="https://gcms.labrulez.com/webinar/3009">Registration</a></li>
</ul>
<p>Water injections and not only how to perform them correctly, but also the many pointers to keep in mind when working with water in GC.</p>
<h5><strong>6. Agilent Technologies: Microplastic detection and quantification in the Cincinnati urban watershed using laser direct infrared (LDIR) chemical imaging system</strong></h5>
<ul>
<li>Tu, 27.2.2024, 21:30 (CEST)</li>
<li><a href="https://gcms.labrulez.com/webinar/3217">Registration</a></li>
</ul>
<p>Microplastic detection and quantification in the Cincinnati urban watershed using laser direct infrared (LDIR) chemical imaging system.</p>


Webinars LabRulezGCMS Week 8-9/2024

<p><strong>In the week from 19th of February, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p>
<blockquote>
<h4><a href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Tosoh Bioscience: Tosoh Tuesday - Economic Model of Protein A Multi-Column Chromatography</strong></h5>
<ul>
<li>Tu, 20.2.2024, 15:00 CET</li>
<li><a href="https://lcms.labrulez.com/webinar/3569">Registration</a></li>
</ul>
<p>Multi-column chromatography (MCC) can be used to intensify Protein A chromatography in a continuous mode, increasing productivity threefold or more.</p>
<h5><strong>2. Waters Corporation: Therapeutic Oligonucleotide Analysis from Plasma &amp; Tissues Samples using OligoWorks SPE Kits</strong></h5>
<ul>
<li>Tu, 20.2.2024, 17:00 CET</li>
<li><a href="https://lcms.labrulez.com/webinar/3532">Registration</a></li>
</ul>
<p>In this webinar, Waters application development scientists will present new HRMS full scan data on plasma samples, as well as HRMS and Tandem Quad data from liver samples.</p>
<h5><strong>3. Agilent Technologies: HPLC Method Development: From Beginner to Expert, Part 1</strong></h5>
<ul>
<li>Tu, 20.2.2024, 17:00 (CEST)</li>
<li><a href="https://lcms.labrulez.com/webinar/3002">Registration</a></li>
</ul>
<p>Basic chromatographic principles to advanced method development topics.</p>
<h5><strong>4. Separation Science/Biotage: Optimization of PFAS analysis in fish tissue samples through a simplified and automated sample preparation workflow</strong></h5>
<ul>
<li>We, 21.2.2024, 11:00 CET</li>
<li><a href="https://lcms.labrulez.com/webinar/3427">Registration</a></li>
</ul>
<p>This webinar demonstrates a homogenization, extraction, and concentration workflow that provides exceptional precision, accuracy, and detection limits for PFAS in fish tissue samples.</p>
<h5><strong>5. Agilent Technologies: Peptide Mapping: Decoded</strong></h5>
<ul>
<li>We, 21.2.2024, 11:30 (CEST)</li>
<li><a href="https://lcms.labrulez.com/webinar/2698">Registration</a></li>
</ul>
<p>What is peptide mapping? How many types of post-translational modifications can be analyzed?</p>
<h5><strong>6. Agilent Technologies: Acceptability and Antioxidant Properties of Vit D and Saskatoon Berry Fortified Rooibos Tea</strong></h5>
<ul>
<li>We, 21.2.2024, 20:00 CET</li>
<li><a href="https://lcms.labrulez.com/webinar/3364">Registration</a></li>
</ul>
<p>This webinar highlights the development of an iced-rooibos tea with Vitamin D3 and Saskatoon berry syrup fortification as a functional beverage</p>
<h5><strong>7. The Analytical Scientist/Phenomenex: Method Robustness and Transferability for Modern Size-Exclusion Methods</strong></h5>
<ul>
<li>Th, 22.2.2024, 11:00 CET</li>
<li><a href="https://lcms.labrulez.com/webinar/3480">Registration</a></li>
</ul>
<p>In this presentation, we discuss SEC method experimental design space. Column selection, mobile phase, flowrate, and injection volume are all discussed.</p>
<h5><strong>8. Agilent Technologies: Evolving Landscape on Control of Excipients Quality in Pharmaceutical Products</strong></h5>
<ul>
<li>Th, 22.2.2024, 12:00 CET</li>
<li><a href="https://lcms.labrulez.com/webinar/3535">Registration</a></li>
</ul>
<p>Join this webinar to hear IPC, USP and Agilent experts share their views on the role of Pharmacopeia&#39;s in ensuring Quality of Pharmaceutical products and excipients.</p>
<h5><strong>9. Agilent Technologies: Quantitative Analysis of Mycotoxins in Foods by Triple Quadrupole LC/MS</strong></h5>
<ul>
<li>Th, 22.2.2024, 14:00 CET</li>
<li><a href="https://lcms.labrulez.com/webinar/3425">Registration</a></li>
</ul>
<p>We will explore Agilent&#39;s range of potential end-to-end answers for a wide variety of foodstuffs to help laboratories with the analysis of mycotoxins.</p>
<h5><strong>10. Agilent Technologies:: Expert Insights into Dissolution Testing of Generic Pharmaceutical Products</strong></h5>
<ul>
<li>Th, 22.2.2024, 17:00 CET</li>
<li><a href="https://lcms.labrulez.com/webinar/3576">Registration</a></li>
</ul>
<p>What could be a better indicator to assure the quality of the generic product than dissolution testing? Join the meeting and make this an interesting discussion with your questions and insights.</p>


Webinars LabRulezLCMS Week 8/2024

<p><strong>In the next 14 days, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p>
<blockquote>
<h4><a href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Bruker Daltonics: Advancements in Operando NMR Analysis of Lithium-Ion Batteries</strong></h5>
<ul>
<li>We, 21.2.2024, 17:00 CET</li>
<li><a href="https://icpms.labrulez.com/webinar/3575">Registration</a></li>
</ul>
<p>Join Dragonfly Energy, Darmstadt Technical University, ePROBE and BRUKER BioSpin as they discuss an experimental procedure for application of in-situ Solid-State NMR for battery research and manufacturing.</p>
<h5><strong>2. Wiley: Introduction to KnowItAll for IR &amp; Raman (Vibrational Spectroscopy)</strong></h5>
<ul>
<li>Th, 22.2.2024, 16:00 CET</li>
<li><a href="https://icpms.labrulez.com/webinar/3570">Registration</a></li>
</ul>
<p>We will demonstrate how KnowItAll can help solve unknown IR and Raman spectra through a series of examples.</p>
<h5><strong>3. Agilent Technologies: Tips and Tricks Workshop on ICP-OES: Smarter Method Development</strong></h5>
<ul>
<li>Th, 22.2.2024, 19:00 (CEST)</li>
<li><a href="https://icpms.labrulez.com/webinar/3160">Registration</a></li>
</ul>
<p>Techniques on how to achieve the best sensitivity and detection limits through tuning techniques and strategies.</p>
<h5><strong>4. Agilent Technologies: See Through the Barrier: The Power of Agilent&#39;s Solutions for Raw Material Verification</strong></h5>
<ul>
<li>Fr, 23.2.2024, 19:00 CET</li>
<li><a href="https://icpms.labrulez.com/webinar/3354">Registration</a></li>
</ul>
<p>This talk will delve into the prowess of these Agilent Spatially Offset Raman spectrometers, emphasizing their capability to ensure material authenticity even through obstructions.</p>
<h5><strong>5. Agilent Technologies: Microplastic detection and quantification in the Cincinnati urban watershed using laser direct infrared (LDIR) chemical imaging system</strong></h5>
<ul>
<li>Tu, 27.2.2024, 21:30 (CEST)</li>
<li><a href="https://icpms.labrulez.com/webinar/3217">Registration</a></li>
</ul>
<p>Microplastic detection and quantification in the Cincinnati urban watershed using laser direct infrared (LDIR) chemical imaging system.</p>
<h5><strong>6. Agilent Technologies: Troubleshooting Tips and Tricks in Single Quad and Triple Quad ICP-MS</strong></h5>
<ul>
<li>Fr, 1.3.2024, 18:00 (CEST)</li>
<li><a href="https://icpms.labrulez.com/webinar/3143">Registration</a></li>
</ul>
<p>Most common tips and tricks on troubleshooting Agilent ICPMS, both single quad and triple quad.</p>


Webinars LabRulezICPMS Week 8-9/2024

<p>🎤 <strong>Presenter</strong>: Christina Kelly, Joseph Binkley, John Hayes (LECO Corporation, Saint Joseph, USA)</p>
<p>💾 <a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/O_12_Characterization_and_Quantitative_Hydrocarbon_Group_Type_Analysis_of_Plastic_Derived_Pyrolysis_Oils_by_G_Cx_GC_TOFMS_FID_0a253a5d5e/O-12-Characterization-and-Quantitative-Hydrocarbon-Group-Type-Analysis-of-Plastic-Derived-Pyrolysis-Oils-by-GCxGC-TOFMS-FID.pdf"><strong>PDF presentation</strong></a></p>
<blockquote>
<p>💡 <strong>Book in your calendar: <a href="https://gcms.labrulez.com/events/518">16th Multidimensional Chromatography Workshop (MDCW) 3 - 5. February 2025</a></strong></p>
</blockquote>
<h3><strong>Abstract</strong></h3>
<p>An increase in the desire for waste <strong>plastics</strong> converted to <strong>pyrolysis oils</strong> to be used as <strong>fuel</strong> has led to the development of processes that create new potential feedstocks which, even with similar physical characteristics to traditional geochemical sources, carry <strong>higher risks of poisoning catalysts</strong> or otherwise reducing efficiency due to very different chemical characteristics.</p>
<p>This presentation focuses on <strong>characterization of plastic-derived pyrolysis</strong> oils and the comparison of polar-nonpolar and nonpolar-polar column sets that target increased chromatographic resolution in different regions of the structured chromatograms, revealing <strong>clusters of compound classes not typically seen in traditional petroleum fuels</strong>, such as multi-branched paraffins and specific heteroatom-containing species of interest.</p>
<p>The data provided by the <strong>LECO Paradigm Shift</strong> highlights the flexibility of this <strong>flow-modulator-and-splitter system</strong> for <strong>comprehensive two-dimensional chromatography</strong> (<strong>GC×GC</strong>) method optimization while utilizing the combined power of <strong>time-of-flight mass spectrometry</strong> (<strong>TOFMS</strong>) and simultaneous <strong>flame ionization detection</strong> (<strong>FID</strong>). With a splitter design ensuring that a constant ratio of analytes is sent to each detector throughout the GC temperature program to maintain uniform area % quantitation from the FID, the <strong>Paradigm Shift system preserves the integrity of the GC×GC separation</strong> of plastic-derived pyrolysis oils for analytical methods that concurrently provide <strong>rich qualitative and accurate quantitative information</strong>.</p>


GC×GC-TOFMS/FID hydrocarbon group-type analysis of plastic-derived oils (Christina Kelly, MDCW 2024)

<p>🎤 <strong>Presenter</strong>: Pierre-Hugues Stefanuto (Liege University, Liege, Belgium)</p>
<p>💾 <a href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/O_17_23_STEFANUTO_1ac93a3857/O-17-!23_STEFANUTO.pdf"><strong>PDF presentation</strong></a></p>
<blockquote>
<p>💡 <strong>Book in your calendar: <a href="https://gcms.labrulez.com/events/518">16th Multidimensional Chromatography Workshop (MDCW) 3 - 5. February 2025</a></strong></p>
</blockquote>
<h3><strong>Abstract</strong></h3>
<p>According to the <strong>world health organization</strong> (<strong>WHO</strong>), <strong>colorectal cancer</strong> (<strong>CRC</strong>) ranks as the third most frequently diagnosed cancer and the <strong>second leading cause of cancer-related deaths</strong>. The current endoscopic-based or stool-based diagnostic techniques are either highly invasive or lack sufficient sensitivity. Thus, there is a <strong>need for better screening approaches</strong>.</p>
<p>The rapid advancement of <strong>high throughput</strong> “<strong>omics</strong>” approaches, such as meta-genomics, transcriptomics, proteomics, metabolomics, lipidomics, microbiomics, and volatolomics, offer a potentially <strong>less invasive alternative</strong> than available techniques to develop <strong>novel biomarkers for colorectal cancer screening</strong> that could contribute to its clinical management.</p>
<p>For small molecules “omics” screening, <strong>gas chromatography</strong> coupled to <strong>mass spectrometry</strong> (<strong>GC-MS</strong>) represents a method of choice to <strong>screen biofluid samples</strong>. However, GC-MS is mostly used for targeted analysis due to the high complexity of the matrices. To tackle this limitation, <strong>comprehensive two-dimensional gas chromatography coupled to mass spectrometry</strong> provided increased separation capabilities, making it a <strong>method of choice for untargeted small molecule metabolomics</strong>.</p>
<p>In this study, we analyzed 64 human serum samples representing three different groups of colorectal cancer using cutting edge <strong>GC×GC–LR/HR-TOFMS</strong> techniques. We analyzed samples with two different specifically tailored sample preparation approaches for lipidomics (fatty acids) (25 μL serum) and metabolomics (50 μL serum). In-depth <strong>chemometric screening</strong> with <strong>supervised</strong> and <strong>unsupervised approaches</strong> and <strong>metabolic pathway analysis</strong> were applied on both datasets. To ensure the high quality of the data produced, we have established a strict <strong>QAQC protocol</strong> including pooled sampled, serum SRM, and standard mixtures injections.</p>


Multi-omics workflow for blood serum screening using GC×GC-TOFMS (Pierre-Hugues Stefanuto, MDCW 2024)

<p><strong>Associate Professor <a href="https://lcms.cz/articles?search=Pavel+Po%C5%99%C3%ADzka"><strong>Pavel Pořízka</strong></a> is a member of Prof. Kaiser&#39;s research group, <em>Advanced Instrumentation and Methods for Materials Characterization</em>, at CEITEC BUT. Although the researchers mostly focus on the development of instrumentation and methodology in collaboration with industry, they have recently started to explore applications in biology thanks to a junior grant. In a GAČR-funded project entitled <em>Processes of laser ablation of soft tissues and subsequent development of laser-excited plasmas</em>, Pořízka and his team focused on the analysis of soft tissues using LIBS (Laser-Induced Breakdown Spectroscopy).</strong></p>
<h3><strong>The completed project was rated as excellent; it was also the first grant you led. Can you tell us more about it?</strong></h3>
<p>That was under a programme that I am happy to talk about and of which I was a successful applicant. It&#39;s a good thing that grant agencies provide individual calls for young scientists at the beginning of their careers, give them the opportunity to participate in a grant competition and gain valuable experience in managing larger projects. The grant we are talking about was announced by the Czech Science Foundation (Grantová agentura České republiky, GAČR) in the GAČR JUNIOR call (which gradually transformed into the larger JUNIOR STAR call). It&#39;s basically financial support for budding scientists. One of the conditions for applying for this grant was a long-term internship abroad, so I went to the University of Florida under a Fulbright grant.</p>
<p>Thanks to the junior grant, I had the opportunity to build my own team focusing only on the analysis of biological samples and to be its principal investigator. Until then, I was mainly a co-investigator on grants within our research group. As was the case of the previous grant awarded to my colleague Pavlina Modlitbová, which was again a grant for young researchers, TAČR – ZÉTA programme. This grant focused mainly on applied research, but it was in the field of biology, too.</p>
<h3><strong>What do you see as the biggest advantage of these programmes that support scientists at the beginning of their careers?</strong></h3>
<p>They literally get careers off the ground. Usually, if you haven&#39;t had a grant and you haven&#39;t been a principal investigator, you have little chance of getting a grant, which creates a kind of vicious circle. These programmes are supposed to disrupt that established system, and I&#39;m glad it&#39;s working that way. If there is even more intensive funding for grants for early-career scientists and, in particular, female scientists, that will only be a good thing. Thanks to this programme, we have received follow-up grants that have helped us to enter other scientific fields. It really is a great helping hand at the beginning of a career.</p>
<h3><strong>How does the process of selecting winning projects work?</strong></h3>
<p>Practically, it&#39;s a standardized procedure. The project is evaluated by a GAČR panel, where the committee and the opponents give their opinions on it. It is not simply included in the regular GAČR cycle with standard projects; it is judged outside of it. As a result, the project is evaluated independently of other project applications submitted by experienced scientists with a &quot;better&quot; CV. The h-index has long been crucial from the GAČR&#39;s point of view but this is also changing. At the beginning of one&#39;s career, one has a less impressive CV, and when one has to compete with someone who has a successful 20-year-long scientific career and has already received several research grants in the past, it is hard to get ahead.</p>
<h3><strong>Why did you take an interest in biological applications?</strong></h3>
<p>After returning from my internship abroad as part of my PhD studies at BUT, in our research group, we started to develop not only the development of instrumentation, which was historically strong here, but also the possible applications. This direction was interesting for several reasons. The most important factor was that it was a topic that I was personally interested in, especially in terms of the difficulty of analysing the sample itself, interpreting the data, and optimizing the parameters of the measurement setup. It was also something that pushed our collaboration towards developmental biology and, in time, perhaps clinical research. Thanks to this, we are collaborating with the Academy of Sciences and clinical departments at the University Hospitals in Hradec Králové and Ostrava. Last but not least, we have moved on to another grant and it has also brought us new contacts abroad.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Pavel_Porizka_Diky_juniorskemu_grantu_jsme_ziskali_nove_projekty_i_zahranicni_spolupraci_Desticky_fe04570b77/CEITEC-Pavel-Porizka-Diky-juniorskemu-grantu-jsme-ziskali-nove-projekty-i-zahranicni-spolupraci-Desticky.jpg" alt="CEITEC: Pavel Pořízka: We have obtained new projects and foreign collaboration thanks to a junior grant"/></p>
<h3><strong>Can you highlight any foreign cooperation?</strong></h3>
<p>The strongest foreign cooperation in the field of biology is with the research group of Professor Andreas Limbeck from the Technical University of Vienna and Associate Professor Gabriela Kalčíková from the University of Ljubljana. We have a joint grant with both on the detection of microplastics in soft tissues and their influence and toxicity in these tissues. To sum it all up, this was a strategic and key decision for us – we have expanded our existing applications to include biology, and this has taken us further. We&#39;ve created a smaller team of about five or six students and two postdocs that are just focused on biological applications, whether we&#39;re talking about plant analysis, hard tissue (teeth and bones), or soft tissue (organs).</p>
<h3><strong>What is the potential of LIBS in the study of tumour tissues?</strong></h3>
<p>LIBS is a method of analytical chemistry designed for elemental analysis that is applied in a wide range of fields, including biology, but also, for example, geology. The method allows the distribution of elements on the surface of a prepared sample to be measured. In clinical research, we are investigating whether the LIBS method can be used to diagnose and support pathology and standard procedures currently used to refine the estimation of the extent of cancer in human skin. This is our main hypothesis. If we are able to refine the estimation of the area of diseased tissue, this could be used for diagnostic purposes during surgical procedures in the future. However, this remains a vision for now.</p>
<h3><strong>How far are you?</strong></h3>
<p>At the moment, we have already obtained dozens of samples from the hospital, and we have permission from the ethics committee to study them. The analysis of these samples is ongoing and we are preparing several scientific articles to present our research achievements. We also participate in international conferences where we receive positive feedback. This support convinces us that our work is worthwhile and that we should continue. We are focusing on combining different approaches from the perspective of correlative microscopy, which means combining classical microscopy and spectroscopy to use different techniques to obtain the most comprehensive information about the samples, i.e. how the chemical and structural composition changes depending on the disease being studied.</p>
<h3><strong>Where would you like to take the research in the near future?</strong></h3>
<p>We are moving forward and would like to submit another grant in the framework of Czech-Austrian cooperation in collaboration with the Technical University of Vienna. This will be a follow-up grant focusing on human tissue analysis and the study of skin cancer. But again, I have to stress that the beginning of this journey was the junior grant mentioned above which opened the door for us both in soft tissue analysis and international cooperation. Without this support, we would not have been able to successfully continue to discover new applications and expand the use of our method.</p>
<h3><strong>So you have analysed mouse and pig tissues first?</strong></h3>
<p>Clinical samples are very valuable to us, so we have model tissues – organs from laboratory mice – that we use to simulate human tissue. We don&#39;t measure clinical samples straight away, we first have to check if the system itself is working at maximum performance. In fact, when we analyse samples with LIBS, we use a powerful laser pulse to slightly heat and vaporise the material, creating the necessary analytical glowing plasma, thus practically destroying the sample. So the thin histological sections we get from the hospital are gone after the measurements.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Pavel_Porizka_Diky_juniorskemu_grantu_jsme_ziskali_nove_projekty_i_zahranicni_spolupraci_lab_2289946f37/CEITEC-Pavel-Porizka-Diky-juniorskemu-grantu-jsme-ziskali-nove-projekty-i-zahranicni-spolupraci-lab.jpg" alt="CEITEC: Pavel Pořízka: We have obtained new projects and foreign collaboration thanks to a junior grant"/></p>
<h3><strong>Can we say that you are pioneers in measuring tumour tissue with LIBS?</strong></h3>
<p>I wouldn&#39;t say that at all. There are a few labs in the global community working on similar topics. We are slightly inspired by the success of our French colleagues at the University of Lyon. The LIBS method is not as established as competing methods. This is what makes the whole research more interesting, as we have to discover a lot of things ourselves. We are trying to move it closer to standard, routine use. So I wouldn&#39;t say pioneers, but I think we are making a significant contribution to making it more widely adopted and more competitive with other techniques.</p>
<h3><strong>Does the startup you founded in 2019, Lightigo, help you do that?</strong></h3>
<p>Extremely. It helps us develop systems that are not commercially available in the world, which was one of the most significant limitations of the whole method. This is something where we see ourselves quite at the forefront – on the one hand, we are developing the actual method of how to measure samples, and on the other hand, we are also able to develop systems in broad collaboration with our startup. This connection gives us the unique ability to adapt the system according to how it should measure, what performance it should have, which also makes it possible for us to move forward faster. This way, we are not dependent on any third-party instrumentation. However, it&#39;s more challenging in that we need broader funding.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CEITEC_Pavel_Porizka_Diky_juniorskemu_grantu_jsme_ziskali_nove_projekty_i_zahranicni_spolupraci_2f1c1f9224/CEITEC-Pavel-Porizka-Diky-juniorskemu-grantu-jsme-ziskali-nove-projekty-i-zahranicni-spolupraci.jpg" alt="CEITEC: Pavel Pořízka: We have obtained new projects and foreign collaboration thanks to a junior grant"/></p>
<h3><strong>How demanding, financially speaking, is LIBS compared to competing methods?</strong></h3>
<p>The instrumentation and the low cost of the method are some of its biggest advantages. Our system is significantly cheaper than competing techniques, such as LA-ICP-MS, but lacks the sensitivity that seems to be essential in some applications. It is sufficient In applications such as the detection of heavy metals in teeth and bone. Although the LIBS method cannot be adopted in all applications, it can be used in many cases and achieve both financial and time savings. The limitations of the method were one of the aspects we addressed in the JUNIOR grant.</p>
<h3><strong>Are there any applications where you have already got into “hot water”?</strong></h3>
<p>There are some but that makes it all the more interesting to try. For example, we have not yet been able to detect iodine, which is used to stain the sample before computed tomography, i.e. before structural analysis. But we don&#39;t see it as a failure. It shouldn&#39;t sound like that, because if we can&#39;t do something now, it doesn&#39;t mean that we won’t be able to do it in the future with further development of instrumentation and measurement methodology.</p>
<h3><strong>What is the priority project now?</strong></h3>
<p>Right now, the three most important projects for us are the three GAČR projects and other TAČR projects in the TREND and NCK programmes. So we have a number of ongoing projects that involve the development of instrumentation and application in industry. However, we are able to manage such a broad scope because we have a group of talented students in our lab who help us significantly in moving forward quickly. They work on sub-topics and actively contribute to the development of our method during their studies.</p>
<p>Two GAČR projects are focused directly on biology, meaning we are able to follow our strategy. In the first project, we are starting our third year of work, and it concerns the detection of nanoparticles as indirect biomarkers of human tissue. With this method, we can specifically label the defined tissue, for example, breast cancer. Another priority for us is the detection of microplastics in soft tissues, where we combine LIBS, LA-ICP-MS and Raman spectroscopy for chemical analysis. We complement these spectroscopic and spectrometric methods with structural analysis by computed tomography from the laboratory of Assoc. Tomáš Zikmund, who is also part of Prof. Kaiser&#39;s research group. Our aim is to find out how the presence of microplastics affects the surrounding soft tissue and whether their presence can have any negative (toxic) effects. Trace elements are commonly found in plastics, including some toxic ones such as lead and cadmium. These substances can diffuse into the surrounding tissue and possibly affect it.</p>


Pavel Pořízka: We have obtained new projects and foreign collaboration thanks to a junior grant

<p><strong>In the week from 26th of February, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p>
<blockquote>
<h4><a href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Agilent Technologies: Harmonized USP Chapter 621 Chromatography – Recent Revisions</strong></h5>
<ul>
<li>Tu, 27.2.2024, 07:00 CET</li>
<li><a href="https://gcms.labrulez.com/webinar/3573">Registration</a></li>
</ul>
<p>In this webinar we will give an overview of General Chapter &lt;621&gt; Chromatography and the allowed adjustments to chromatographic conditions in the USP monographs. </p>
<h5><strong>2. Agilent Technologies: Navigating Troubled Waters: A Deep Dive into Water Injections by GC</strong></h5>
<ul>
<li>Tu, 27.2.2024, 17:00 CET</li>
<li><a href="https://gcms.labrulez.com/webinar/3009">Registration</a></li>
</ul>
<p>Water injections and not only how to perform them correctly, but also the many pointers to keep in mind when working with water in GC.</p>
<h5><strong>3. Agilent Technologies: Microplastic detection and quantification in the Cincinnati urban watershed using laser direct infrared (LDIR) chemical imaging system</strong></h5>
<ul>
<li>Tu, 27.2.2024, 21:30 CET</li>
<li><a href="https://gcms.labrulez.com/webinar/3217">Registration</a></li>
</ul>
<p>Microplastic detection and quantification in the Cincinnati urban watershed using laser direct infrared (LDIR) chemical imaging system.</p>


Webinars LabRulezGCMS Week 9/2024

<p><strong>In the week from 26th of February, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p>
<blockquote>
<h4><a href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Agilent Technologies: Microplastic detection and quantification in the Cincinnati urban watershed using laser direct infrared (LDIR) chemical imaging system</strong></h5>
<ul>
<li>Tu, 27.2.2024, 21:30 CET</li>
<li><a href="https://icpms.labrulez.com/webinar/3217">Registration</a></li>
</ul>
<p>Microplastic detection and quantification in the Cincinnati urban watershed using laser direct infrared (LDIR) chemical imaging system.</p>
<h5><strong>2. Agilent Technologies: Troubleshooting Tips and Tricks in Single Quad and Triple Quad ICP-MS</strong></h5>
<ul>
<li>Fr, 1.3.2024, 18:00 CET</li>
<li><a href="https://icpms.labrulez.com/webinar/3143">Registration</a></li>
</ul>
<p>Most common tips and tricks on troubleshooting Agilent ICPMS, both single quad and triple quad.</p>


Webinars LabRulezICPMS Week 9/2024

<p><strong>In the week from 26th of February, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p>
<blockquote>
<h4><a href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter in the largest global database</strong>  with over <strong>3 500 GC, LC, MS and spectroscopy webinars</strong>.</p>
</blockquote>
<h5><strong>1. Agilent Technologies: Harmonized USP Chapter 621 Chromatography – Recent Revisions</strong></h5>
<ul>
<li>Tu, 27.2.2024</li>
<li><a href="https://lcms.labrulez.com/webinar/3573">Registration</a></li>
</ul>
<p>In this webinar we will give an overview of General Chapter &lt;621&gt; Chromatography and the allowed adjustments to chromatographic conditions in the USP monographs. </p>
<h5><strong>2. Bruker Daltonics: Application of Lipidomics to Exploratory Discovery in Metabolic Diseases</strong></h5>
<ul>
<li>Tu, 27.2.2024</li>
<li><a href="https://lcms.labrulez.com/webinar/3567">Registration</a></li>
</ul>
<p>Join us for a deep dive into lipidomics, exploring its power to unlock the mysteries of metabolic disease.</p>
<h5><strong>3. Waters Corporation: 5 Rules for Successfully Developing and Scaling Up Your LC Purification</strong></h5>
<ul>
<li>We, 28.2.2024</li>
<li><a href="https://lcms.labrulez.com/webinar/3572">Registration</a></li>
</ul>
<p>A review of scale-up rules as well as practical guidance for first-time purification success will be the focus of this webinar.</p>
<h5><strong>4. Labcompare/Waters Corporation: Leveraging Advanced Analytical Technologies to Expedite the Development of Antibody Drug Conjugates</strong></h5>
<ul>
<li>We, 28.2.2024</li>
<li><a href="https://lcms.labrulez.com/webinar/3482">Registration</a></li>
</ul>
<p>Join us on an exploration of the analytical characterization required to confidently assess the ID, safety and efficacy of an antibody drug conjugate (ADC).</p>
<h5><strong>5. Shimadzu Corporation: Advancing Newborn Screening with MS Technology</strong></h5>
<ul>
<li>Th, 29.2.2024</li>
<li><a href="https://lcms.labrulez.com/webinar/3568">Registration</a></li>
</ul>
<p>Join us as we delve into the potential of MS/MS technology in advancing newborn screening and explore Shimadzu&#39;s innovative Analytical and Medical solutions.</p>
<h5><strong>6. Evosep Biosystems: Evosep One in the Proteomics Core Facility</strong></h5>
<ul>
<li>Th, 29.2.2024</li>
<li><a href="https://lcms.labrulez.com/webinar/3534">Registration</a></li>
</ul>
<p>Join our webinar and learn more of how the Evosep technology, its workflows and applications in the Proteomics Core Facility.</p>
<h5><strong>7. Waters Corporation: Why we love Multi-Angle Light Scattering! How MALS works and what it measures. Modes of operation and when to use them</strong></h5>
<ul>
<li>Fr, 1.3.2024</li>
<li><a href="https://lcms.labrulez.com/webinar/3607">Registration</a></li>
</ul>
<p>Why we love Multi-Angle Light Scattering! How MALS works and what it measures. Modes of operation and when to use them.</p>


Webinars LabRulezLCMS Week 9/2024

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

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CEITEC

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The Multidimensional Chromatography Workshop

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Multi-omics workflow for blood serum screening using GC×GC-TOFMS (Pierre-Hugues Stefanuto, MDCW 2024)

Pavel Pořízka: We have obtained new projects and foreign collaboration thanks to a junior grant

GC×GC-TOFMS/FID hydrocarbon group-type analysis of plastic-derived oils (Christina Kelly, MDCW 2024)

Webinars LabRulezICPMS Week 9/2024

Webinars LabRulezGCMS Week 9/2024

Webinars LabRulezLCMS Week 9/2024

Webinars LabRulezGCMS Week 8-9/2024

Webinars LabRulezICPMS Week 8-9/2024

Webinars LabRulezLCMS Week 8/2024

Nexera HPLC and Nexis GC systems Supported in Clarity

Upcoming events

LECO Training Course Pegasus BT/BT4D BTX/BTX4D

Thermo Fisher Scientific Mass Spectrometry Users’ Meetings 2026

Bruker at HUPO 2026

Webinars

Why fast, reliable trace‑element analysis starts with triple‑quad ICP‑MS

Scalable HPLC workflows for linking antibody productivity, titer, and CQAs

Vial Selection, Qualification, and the Evidence Behind the Decision