Forced degradation analysis of recombinant adeno-associated virus serotype 8 based on analytical anion exchange chromatography coupled to orthogonal characterization

Oligonucleotide and mRNA Analysis Resource Roundup

<p><strong>NIST26 introduces integrated chromatogram processing, deconvolution, and library searching for EI GC-MS and LC-MS/MS data, but the workflow begins with one essential requirement: chromatographic data must be available in the mzML format. In his presentation </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/Conversion_to_mz_ML_63e134bb34.pdf"><i><strong>Conversion to mzML Format for NIST26 Chromatogram Processing</strong></i></a><strong>, James Little of Mass Spec Interpretation Services explains how vendor data can be converted to mzML using either instrument-vendor software or the freely available MSConvert tool from ProteoWizard, and highlights several settings that are critical for successful processing in NIST26.</strong></p><h4><span><strong>NIST26 Chromatogram processing requires mzML</strong></span></h4><p>For the chromatogram-processing workflow described in the presentation, <strong>mzML is the accepted input format</strong>. Proprietary LC-MS or GC-MS data therefore need to be converted before they can be loaded into the NIST26 chromatogram-processing environment.</p><p>There are two principal ways to obtain an mzML file:</p><ul><li>conversion using software supplied by the instrument manufacturer,</li><li>conversion using <strong>MSConvert</strong>, which is included in the free <strong>ProteoWizard</strong> software package.</li></ul><p>Using an open format such as mzML also provides a practical way to move mass spectrometry data between different software environments without relying exclusively on proprietary vendor formats.</p><h4><span><strong>Using ProteoWizard and MSConvert</strong></span></h4><p>MSConvert is installed as part of the complete ProteoWizard package rather than as a separate application. After installing ProteoWizard, users can access either the graphical MSConvert interface or the command-line version.</p><p>The presentation recommends the stable release for routine work, while noting that newer or nightly builds may sometimes provide improved support for recently introduced vendor formats or additional control over data-processing filters. This can be particularly relevant when handling vendor files in which centroiding behavior or peak picking requires closer attention.</p><p>For most users, the graphical interface provides a straightforward route from proprietary raw data to an mzML file suitable for further NIST26 processing.</p><h4><span><strong>Start by selecting mzML as the output format</strong></span></h4><p>The first step in MSConvert is to select the source file or dataset using the <strong>Browse</strong> function.</p><p>The software supports a broad range of mass spectrometry data formats, and the file-selection window can either be restricted to a particular vendor format or set to display any supported spectral format.</p><p>After selecting the source data, the output format must be set to <strong>mzML</strong>. The screenshots in the presentation show this setting directly in the MSConvert options panel before the conversion is started.</p><p>The user can also select the destination folder and other file-output options before adding the selected dataset to the conversion queue.</p><h4><span><strong>Add the data and start the conversion</strong></span></h4><p>Once the source file and output settings have been selected, the file is added to the conversion list using the <strong>Add</strong> command.</p><p>The conversion itself is then launched with <strong>Start</strong>.</p><p>For simple datasets that are already stored in the correct spectral representation, this may be all that is required. However, James Little emphasizes an additional requirement that is particularly important for NIST26: the data must be <strong>centroided</strong>.</p><h4><span><strong>Centroided data are required</strong></span></h4><p>According to the presentation, <strong>profile-mode data are not acceptable for this NIST26 chromatogram-processing workflow</strong>. The spectra must instead be in centroided, or peak-picked, form.</p><p>Profile spectra contain the full measured shape of each mass spectral signal across the m/z axis. Centroided data reduce these profiles to discrete peaks represented by their characteristic m/z and intensity values.</p><p>If the original vendor data are still in profile mode, centroiding can be performed during conversion by adding the <strong>Peak Picking</strong> filter in MSConvert.</p><p>This means that the conversion step can perform two operations at once:</p><p><strong>vendor raw data → centroiding / peak picking → mzML</strong></p><p>The resulting mzML file can then be used as input for NIST26 chromatogram processing.</p><h4><span><strong>Peak Picking must be the first filter</strong></span></h4><p>One of the most important practical details in the presentation is the position of the Peak Picking operation in the MSConvert filter sequence.</p><p>If centroiding is required, <strong>Peak Picking must be the first algorithm or filter in the list</strong>. Placing another processing function before it can interfere with the intended conversion workflow.</p><p>MSConvert allows the user to select the Peak Picking filter, define the relevant MS levels, and add it to the processing sequence before starting conversion. The example shown in the presentation applies vendor-based peak picking to selected MS levels.</p><p>If there is uncertainty about whether the source data are already centroided, the Peak Picking filter can still be included. According to the presentation, applying it to data that are already centroided does not alter the file unnecessarily.</p><p>This provides a useful safeguard when the acquisition mode of an older dataset is uncertain.</p><h4><span><strong>Selecting the appropriate MS levels</strong></span></h4><p>Peak picking does not necessarily have to be applied identically to every spectrum in a dataset. MSConvert allows users to specify the <strong>MS levels</strong> to which the filter should be applied.</p><p>This is especially relevant for LC-MS/MS data, where a file can contain both precursor-level MS spectra and tandem MS spectra.</p><p>The conversion settings should therefore reflect the way the original data were acquired and how those data will subsequently be processed in NIST26.</p><h4><span><strong>Be aware of changes in file size</strong></span></h4><p>The presentation also notes that converted files may become substantially different in size from the original vendor files.</p><p>This is worth considering when working with large LC-MS/MS datasets or processing batches of chromatograms. The size of the resulting mzML file can depend on the original acquisition mode, centroiding, compression settings, the number of MS levels, and other output parameters.</p><p>Storage requirements should therefore be considered before converting large collections of raw data.</p><h4><span><strong>A simple workflow for preparing data for NIST26</strong></span></h4><p>The overall procedure presented by James Little can be summarized as a short sequence:</p><ol><li>Obtain the original vendor LC-MS or GC-MS data.</li><li>Open the data in MSConvert or use the vendor's own conversion software.</li><li>Select <strong>mzML</strong> as the output format.</li><li>Check whether the spectra are centroided.</li><li>If necessary, add <strong>Peak Picking</strong> as the first processing filter.</li><li>Select the appropriate MS levels.</li><li>Add the file to the conversion list.</li><li>Start the conversion.</li><li>Use the resulting mzML file for NIST26 chromatogram processing.</li></ol><p>The procedure itself is straightforward, but the centroiding requirement is important. A file can have the correct <code>.mzML</code> extension and still be unsuitable for the workflow if its spectra remain in profile mode.</p><h4><span><strong>From vendor data to integrated NIST26 processing</strong></span></h4><p>File conversion may appear to be a relatively minor step compared with deconvolution, spectral interpretation, and library searching, but it establishes the foundation for the entire downstream workflow.</p><p>By using mzML as the common input format, NIST26 can process data originating from different instrument platforms through a more consistent workflow. ProteoWizard and MSConvert provide a practical bridge between proprietary mass spectrometry files and this standardized format.</p><p>The key message from the presentation is therefore simple: <strong>convert to mzML, ensure the spectra are centroided, and place Peak Picking first whenever centroiding is required</strong>. Once those conditions are met, the data are ready for the integrated chromatogram-processing and library-searching capabilities available in NIST26.</p>

Conversion to mzML Format for NIST26 Chromatogram Processing

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SelectScience

Waters Corporation

<p><strong>UV-VIS-NIR can be a powerful analysis technique in battery research and development. Whether it is studying new materials, monitoring electrolytes for degradation, or conducting measurements in situ using fiber optics, UV-VIS-NIR measurement capability can support applications from production to end-of-life recycling.</strong></p><p>Agilent's portfolio of instruments offers the high performance and flexible accessories needed for pioneering new applications.</p><blockquote><p><strong>Presenter:</strong> Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)</p><p>Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</p></blockquote>

Agilent Technologies: UV-VIS-NIR in Battery Development

UV-VIS-NIR in Battery Development

Agilent's portfolio of instruments offers the high performance and flexible accessories needed for pioneering new applications.

<header><p><strong>Join Us for an Engaging Live Webinar on Integrating UV/Vis Spectroscopy Into Your Laboratory Workflows</strong></p></header><main><div><div><p><strong>Program&nbsp;</strong></p><ul><li>Introduction to UV/Vis Spectroscopy</li><li>The Power of Integration</li><li>Live Demonstration</li><li>Customer Use Cases</li><li>Engage &amp; Discuss</li></ul></div></div><div><div><p>Discover how connecting your UV/Vis spectrophotometer with instruments like balances can transform your laboratory processes. By transmitting dissolution data directly to the spectrophotometer, you can streamline workflows and enhance data quality, leading to improved productivity and accuracy.</p></div></div><div><div><div><p>In this informative session, we’ll showcase the power of instrument integration and how it can revolutionize your daily routines. Using real-world case studies from various industries, including battery manufacturing, pharmaceuticals, and food and beverage, you’ll gain valuable insights into optimizing your laboratory workflows.</p><p><strong>Program Highlights</strong></p><ul><li><strong>Introduction to UV/Vis Spectroscopy</strong><br>Understand the basics and applications of UV/Vis spectroscopy in laboratories.</li><li><strong>The Power of Integration</strong><br>Explore the benefits of connecting your UV/Vis spectrophotometer with balances and other instruments.</li><li><strong>Live Demonstration</strong><br>Witness a live demonstration of data transmission from a balance to a UV/Vis spectrophotometer.</li><li><strong>Customer Use Cases</strong><br>Learn about integrated processes used in the battery, pharma, and food &amp; beverage industries.</li><li><strong>Engage &amp; Discuss</strong><br>Participate in a Q&amp;A session where we address your questions and discuss potential applications in your own lab.</li></ul><p>Don’t miss this opportunity to explore innovative solutions that can help you think critically about your own processes and enhance your laboratory's efficiency!</p></div></div></div></main><div><div><p><strong>Target Audience</strong></p><div><p>This webinar is designed for laboratory professionals, scientists, and managers across various industries, including:</p><ul><li>Pharmaceuticals: Quality control and research &amp; development teams looking to improve analytical processes</li><li>Food &amp; Beverage: Quality assurance and product development personnel aiming to enhance product testing efficiency</li><li>Battery Manufacturing: Engineers and technicians interested in optimizing material testing workflows</li><li>Laboratory Managers: Professionals seeking to streamline operations and improve data quality through instrument integration</li></ul><p>Whether you are just starting with UV/Vis spectroscopy or looking to enhance your existing laboratory workflows, this webinar is perfect for you. Register now to secure your spot and take the first step towards a more efficient laboratory.</p><ul><li><span style="padding-right:0px;" id="webinar-session-1"><strong>1. 10. 2025 9:00,&nbsp; English&nbsp;</strong></span><span id="webinar-session-2"><strong><svg width="16" height="16" viewBox="0 0 16 16" fill="none" xmlns="http://www.w3.org/2000/svg"></svg></strong></span></li><li><span id="webinar-session-2"><strong>1. 10. 2025 17:00,&nbsp; English&nbsp;</strong></span></li></ul><blockquote><p><span id="webinar-session-2"><strong>Presenter: </strong></span>Anika Adams (Product Manager, SPG UV/VIS)</p><div><p>Anika Adams is Product Manager for UV/Vis Spectrophotometers at METTLER TOLEDO, where she focuses on driving innovation and optimizing customer workflows. With a degree in business administration and experience spanning software development, mechanical engineering, and production, Anika brings a multidisciplinary perspective to the laboratory business. Her expertise lies in automating and enhancing customer processes, ensuring both product excellence and customer satisfaction.</p><p><strong>Presenter:</strong> Dr. Fabian Müller (Product Manager, SPG UV/VIS)</p><div><div><div><div><p>Dr. Fabian Müller, Ph.D., is an accomplished chemist from the ETH Zürich in Switzerland. After completing his doctoral studies, Dr. Müller joined the team at METTLER TOLEDO as an Application Specialist for UV/Vis spectroscopy, where he quickly established himself as a skilled and knowledgeable professional. His expertise in this area led to his promotion to the position of Product Manager for UV/Vis spectrophotometers.</p></div></div></div></div></div></blockquote></div></div></div>

Mettler Toledo: UV/Vis Integration in Laboratory Workflows 

UV/Vis Integration in Laboratory Workflows 

Join Us for an Engaging Live Webinar on Integrating UV/Vis Spectroscopy Into Your Laboratory Workflows

<div><p><strong>Join us for the last 5 series on building the perfect method for light scattering analysis.</strong></p><p><strong>Learn advanced tools and techniques to master ASTRA software, from normalization to DLS with flowing systems.</strong></p><h4><strong>Session Overview:</strong></h4><ul><li><h5><strong>Part 6 of 10: Building the Perfect Method - Normalization, Alignment, &amp; Inter-Detector Band Broadening Corrections</strong></h5><ul><li>Friday, September 26</li></ul></li><li><strong>Part 7 of 10: </strong>Navigating Sample Parameters – A Close Look at dn/dc and Extinction Coefficients<ul><li>Friday, October 3</li></ul></li><li><strong>Part 8 of 10: </strong>Advanced Characterization Tools for Multi-Component Analytes - Protein Conjugates, AAV, LNP and ADC<ul><li>Friday, October 10</li></ul></li><li><strong>Part 9 of 10: </strong>ASTRA for Particles: When and How to Deploy<ul><li>Friday, October 17</li></ul></li><li><strong>Part 10 of 10: </strong>DLS with Flowing Systems – Leveraging Rh and the Burchard-Stockmayer Plot in ASTRA<ul><li>Friday, October 24</li></ul></li></ul><h4><strong>Why Attend:</strong></h4><ul><li>Deepen your understanding of light scattering and ASTRA.</li><li>Gain actionable insights for your lab.</li><li>Interact with our expert, Dr. Andy Meyer.</li></ul><p>The live interactive web series are 100% live sessions, with anonymous Q&amp;A welcome during and after the discussion. Free for all Wyatt customers.</p><blockquote><p><strong>Hosted by:</strong> Dr. Andy Meyer</p><p>Dr. Andy Meyer joined Wyatt Technology in 2001, serving for several years as an Applications Scientist, then as Dean of Wyatt's "Light Scattering University" and as Director of Customer Service &amp; Support. After managing multiple regions in North America, Dr. Meyer is now a Principal Trainer for Waters | Wyatt Technology. As a Wyatt customer, he completed his Ph.D. in Applied Science – Polymer Chemistry from the College of William and Mary in Virginia.</p></blockquote></div>

Waters Corporation: Becoming an ASTRA Expert

Becoming an ASTRA Expert: Part 6

Learn essential method setup steps in ASTRA: normalization, alignment, and band broadening corrections for optimal accuracy in light scattering experiments.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Biopharmaceuticals based on monoclonal antibodies, lipid nanoparticle-encapsulated mRNA, and transgene-carrying viral vectors, are significantly larger than traditional small-molecule drugs. This size difference requires specialized analytical tools. Size-exclusion chromatography (SEC) is particularly effective for studying proteins and has recently been adapted to analyze complex macromolecular assemblies like mRNA and DNA.</strong></p><p>Join us for this exciting two-part webinar series where Dr. Jelle De Vos from RIC group and Dr. Sonja Schneider from Agilent Technologies will discuss how SEC is evolving to meet the demands of modern biopharma. In Part 2, our experts will share real-world application examples of SEC with light scattering detection, highlighting how this powerful combination is transforming the analysis of complex biopharmaceuticals.</p><h5><strong>Key learning objectives of Part 2:</strong></h5><ul><li>Understand the practical application of SEC for the characterization of:<ul><li>monoclonal antibodies</li><li>mRNA-lipid nanoparticle formulations</li><li>adeno-associated viruses (AAVs)</li><li>virus-like particles (VLPs)</li></ul></li></ul><h5><strong>Who should attend?</strong></h5><ul><li>Scientists and researchers in biotherapeutics R&amp;D using SEC/light scattering.</li><li>Lab and quality leaders, including lab managers, directors, and QA/QC professionals in biopharma production.</li></ul><h5><strong>Want to go back and watch Part 1?</strong></h5><p><strong>Click here to register for Part 1</strong>, and hear the fundamentals of SEC and discover how coupling it with light scattering detection can advance the characterization and development of biopharmaceuticals.</p><h5><strong>Certificate of attendance</strong></h5><ul><li>If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</li><li>If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Jelle De Vos (Senior Scientist, RIC group)</p><p>Dr. Jelle De Vos currently works at the RIC group as an expert in developing chromatography methodologies for analyzing complex biopharmaceutical samples. His work focuses on pushing the boundaries of modern separation science, and he has published over 40 articles on advancing the limits of ultra high-performance liquid chromatography and developing microfluidic technology.</p><p><strong>Presenter:&nbsp;</strong>Dr. Sonja Schneider (Scientist, Application Development, Agilent Technologies)</p><p>Dr. Sonja Schneider is an Application Scientist at Agilent Technologies in Waldbronn, Germany, who has published numerous application notes on the analysis of biopharmaceuticals. After obtaining her PhD in proteomics in 2011, she started as an Application Scientist for Bioanalytics and has been working various kinds of critical quality attributes of monoclonal antibodies, mRNA, and LNPs.&nbsp;</p><p><strong>Moderator:</strong> Olivia Long (Editorial Team, SelectScience)</p></blockquote></div></div></div>

SelectScience: Part 2: Size exclusion chromatography with light scattering for biopharmaceuticals

Part 2: Size exclusion chromatography with light scattering for biopharmaceuticals

Explore real-world SEC with light scattering for antibodies, mRNA-LNPs, AAVs, and VLPs. Insights from Agilent and RIC group experts.

SEC with light scattering for biopharmaceuticals

<p><strong>Dr. Michaela Nebel, Product Manager at Sensolytics GmbH, gives an overview of the theory of localized electrochemical techniques with a focus on Scanning Electrochemical Microscopy (SECM), Scanning Droplet Cell technique (SDC), and Scanning Electrochemical Cell Microscopy (SECCM). Then, application examples from different fields in electrochemistry, including electrocatalysis, corrosion research, and sensors are highlighted.</strong></p><p>In recent years, the rising inclusion of localized electrochemical analysis into classical electrochemical methods has allowed for more comprehensive characterization of sample surfaces from various fields of application.</p><h5><strong>Key learnings of this webinar:</strong></h5><ul><li><strong>Theoretical background of localized electrochemical techniques</strong> with a focus on Scanning Electrochemical Microscopy (SECM), Scanning Droplet Cell technique (SDC), and Scanning Electrochemical Cell Microscopy (SECCM)</li><li><strong>Guidelines for the selection</strong> of a localized technique fitting your individual research application</li><li><strong>Practical considerations</strong> for the use of the respective analysis method</li><li><strong>Selected applications</strong> from different fields of research, including electrocatalysis, corrosion research, and sensors</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Michaela Nebel (Product Manager at Sensolytics GmbH)</p></blockquote>

Metrohm: Introduction to Localized Electrochemical Techniques

Introduction to Localized Electrochemical Techniques

Learn SECM, SDC, and SECCM fundamentals and applications in electrocatalysis, corrosion research, and sensors. Practical guidance from experts.

<p><strong>Traditional wet chemistry methods for food testing tend to be slow and wasteful. In contrast, near-infrared spectroscopy (NIRS) is chemical-free and can be used to determine multiple QC parameters in a few seconds with a single measurement.</strong></p><p>Join us for a free webinar on September 25, where Dr. Nicolas Rühl, Product Manager Spectroscopy, and Andreas Agerer, Application Specialist Spectroscopy at Metrohm International Headquarters, will introduce NIRS and explore its wide range of applications for food quality control.</p><p>In short laboratory demonstrations, the presenters will showcase the analysis of moisture, fat, protein, and other food parameters using the newly released OMNIS NIR Analyzer. Highlighting key features such as intuitive measurement workflows, calibration at the push of a button, and fully automated analysis of more than 200 samples, the presenters will show you how to boost your food testing to a whole new level of performance.</p><p><strong>Don't miss this opportunity and register today!</strong></p><h5><strong>Join us for this event and learn:</strong></h5><ul><li>What NIR spectroscopy is and how it works</li><li>How to perform NIR analysis to determine typical food quality parameters</li><li>How NIR spectroscopy helps you save time and costs of your routine food testing</li><li>How unique features of the OMNIS NIR Analyzer make NIR analysis easy to use for everyone</li></ul><h5><strong>Who should attend?</strong></h5><ul><li>QC and QA managers from the food industry, lab managers, lab technicians</li></ul><blockquote><p><strong>Presenter: </strong>Dr. Nicolas Rühl (Product Manager Spectroscopy, Metrohm International Headquarters)</p><p>Dr. Nicolas Rühl works at the Competence Center Spectroscopy at Metrohm International Headquarters since 2015. In this time, he has been in charge of developing the near-infrared (NIR) spectroscopy business at Metrohm. Nicolas has a Master in Business Administration and received his PhD in physical chemistry on single-molecule spectroscopy using Vis-NIR and Raman spectroscopy technologies.</p><p><strong>Presenter: </strong>Andreas Agerer (Application Specialist NIRS, Metrohm International Headquarters)</p><p>Andreas Agerer is an Application Specialist at Metrohm AG, working in the Competence Center Spectroscopy since 2018. In this time, he has been responsible for developing NIRS methods tailored to new application areas and supporting sales teams during feasibility studies. Andreas completed his university graduate work in the field of polymer analysis using vibrational spectroscopy.</p></blockquote>

Metrohm: Next-generation NIR spectroscopy and how to use it for high-throughput food testing

Next-generation NIR spectroscopy and how to use it for high-throughput food testing

Join Metrohm’s webinar to explore NIR spectroscopy for food QC. See how the OMNIS NIR Analyzer delivers fast, chemical-free analysis of key food parameters.

Next-generation NIR spectroscopy for food testing

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p>Join us for an insightful webinar exploring the Lab of the Future, where emerging technologies like AI, automation and smart lab environments are revolutionizing scientific research. Discover how innovations in lab informatics, robotics and real-time data integration are driving enhanced research efficiency, fostering collaboration and ensuring data integrity. This event is designed for scientists, lab managers and technology leaders eager to learn about the next generation of research spaces and how to harness these advancements for breakthrough discoveries.</p><h5><strong>Attend this webinar to:</strong></h5><ul><li>See how AI and automation boost lab efficiency</li><li>Understand smarter data management with lab informatics</li><li>Discover robotics’ role in accelerating research</li><li>Learn the impact of real-time data integration</li><li>Explore building future-ready smart labs</li></ul><blockquote><p><strong>Presenter: </strong>Nick Birse, PhD (Lecturer, Institute for Global Food Security, Queen's University Belfast)</p><p><strong>Presenter:&nbsp;</strong>Dagnachew (Dan) Birru, PhD (Global Head of Research and Development, Quantiphi)</p><p><strong>Presenter:&nbsp;</strong>Vishal Vaddina (Principal Architect – R&amp;D, Quantiphi)</p><p><strong>Presenter:&nbsp;</strong>Robyn Stoller (Business Development Manager, Cytiva)</p></blockquote></div></div></div>

Technology Networks: Tech Spotlight: Lab of the Future 2025

Tech Spotlight: Lab of the Future 2025

Discover innovations in AI, automation, robotics, and informatics transforming labs into future-ready environments for efficient, collaborative research.

<div><div container-id="cq-colctrl-lt0" comp-id="A1"><div><p><strong>Prof. Thalassinos's research focuses on understanding how cells respond to DNA damage using single-cell proteomics. The study employs a compound called 60G to induce DNA damage, which activates DNA repair mechanisms such as mismatch repair, base excision repair, and nucleotide excision repair. By analyzing single cells, the research aims to uncover the variability in protein expression and the biological processes involved in DNA repair and apoptosis.</strong></p><p>The team uses advanced techniques like the Orbitrap Eclipse mass spectrometer and the SLN1 single-cell isolation system to isolate and analyze individual cells. This approach allows them to observe the upregulation of proteins involved in DNA repair and the arrest of cells in the S phase of the cell cycle. The findings highlight the importance of single-cell proteomics in revealing cellular heterogeneity and the dynamic response to DNA damage, which could have implications for understanding diseases like cancer and neurodegenerative disorders.&nbsp;</p><h5><strong>Learning points:</strong></h5><ul><li><strong>Single-Cell Proteomics Advantage: </strong>Single-cell proteomics allows for the observation of individual cellular responses to DNA damage, revealing heterogeneity that would be masked in bulk proteomics. This approach helps in understanding the specific activation of DNA repair mechanisms and cell cycle arrest at the S phase.&nbsp;</li><li><strong>DNA Repair Mechanisms: </strong>The study highlights the activation of various DNA repair pathways, such as mismatch repair, base excision repair, and nucleotide excision repair, in response to DNA damage induced by the compound 60G. This understanding is crucial for insights into diseases like cancer and neurodegenerative disorders.&nbsp;</li><li><strong>Technological Integration: </strong>The use of advanced technologies like the <strong>Orbitrap Eclipse</strong> mass spectrometer and the SLN1 single-cell isolation system enables precise isolation and analysis of single cells, facilitating detailed proteomic studies and enhancing the understanding of cellular processes at a granular level.&nbsp;</li></ul><h5><strong>Who should attend:</strong></h5><ul><li><strong>Cancer Researchers: </strong>Understanding the variability in DNA repair mechanisms at the single-cell level can provide insights into tumor heterogeneity and the development of targeted cancer therapies.</li><li><strong>Neurodegenerative Disease Scientists</strong>: Insights into DNA damage response mechanisms can aid in understanding the molecular basis of neurodegenerative diseases and potentially lead to new therapeutic strategies.&nbsp;</li><li><strong>Proteomics and Genomics Technologists:</strong> Professionals working with advanced proteomics and genomics technologies can benefit from the presentation's focus on cutting-edge techniques for single-cell analysis, enhancing their research capabilities and methodologies.&nbsp;</li></ul><p>If you cannot attend this webinar please register to receive a link to the On Demand version the following day.&nbsp;</p><blockquote><p><strong>Presenter: </strong>Prof. Konstantinos Thalassinos (Professor, University College London)</p><p>Kostas moved to the UK in 1998 to study for a BSc in Genetics at the University of Leicester, having completed his high school education in his native Greece. After graduation he went on to the University of York to undertake a Masters in Bioinformatics, which included a three-month industrial placement writing software to process mass spectrometric data. Having gained an interest in mass spectrometry, Kostas then went to the University of Warwick in order to pursue a PhD that combined experimental and computational studies in mass spectrometry-based proteomics. After being awarded his PhD in 2006, Kostas was the successful recipient of a one-year Wellcome Trust Value in People Fellowship. Post-doctoral training followed and in 2010 he moved to the Institute of Structural and Molecular Biology at University College London / Birkbeck to take up a lectureship in biophysical mass spectrometry and has been there ever since. In 2015 he was promoted to Senior Lecturer and in 2019 to Professor of mass spectrometry.</p><p>His lab is using structural mass spectrometry approaches, especially ion mobility and crosslinking, to study the structure and dynamics of proteins and protein complexes, particularly those involved in protein misfolding diseases.</p></blockquote></div></div></div>

Thermo Fisher Scientific: ASMS 2025 Workshops

Probing DNA-damage response at the single cell level

Explore single-cell proteomics in DNA damage research. See how Orbitrap Eclipse and SLN1 reveal repair mechanisms, cell cycle arrest, and disease insights.

<p><strong>New functions of the improved UV Workstation Software will be described and demonstrated.</strong></p><blockquote><p><strong>Presenter:&nbsp;</strong> Scott Melis, PhD (Application Scientist, Agilent Technologies, Inc.)</p><p>Scott Melis has a PHD in Physics from Georgetown University in Washington, DC and started with Agilent in 2021 as a Molecular Spectroscopy Application Scientist. In his graduate work, Scott studied nanoparticle formation and growth for a variety of applications. Projects that he worked with include developing processes to deposit and characterize semiconducting molecular nano-cocrystals for use in optoelectronic devices as well as controlling polymer nanoparticle size through rapid mixing conditions.</p></blockquote>

Agilent Technologies: New Software Features for UV Work Station Software

New Software Features for UV Work Station Software

New functions of the improved UV Workstation Software will be described and demonstrated.

<h4><strong>Our newest HPLC columns are game changing</strong></h4><h5><strong>Liquid chromatography is evolving</strong></h5><p>With growing demands for sensitivity, reproducibility, and speed, today’s workflows need column technology that can keep up. Whether you are analyzing trace-level compounds or navigating complex bioseparations, the right column makes all the difference.</p><p>A new generation of LC column technology is setting the bar higher—delivering cleaner separations, sharper peaks, faster equilibration, and more reliable results for even the most metal-sensitive analytes.</p><h5><strong>Want to see it in action?</strong></h5><p>Join our webinar to explore how this new column technology transforms oligonucleotide separations for characterization, sequencing, and impurity profiling.</p><blockquote><p><strong>Presenter:&nbsp;</strong> Dr. Anne Blackwell (Product Manager Bio LC Columns, Agilent Technologies)</p><p>Anne is a product manger for bio LC columns at Agilent, with previous experience as a product support scientist for bio LC columns and an LC/MS applications scientist focused on metabolomics. &nbsp;She has a Ph.D. in analytical chemistry from the University of Arizona where she studied mass spectrometry of non-covalent protein assemblies.</p><p><strong>Presenter: </strong>Dr. Andrew Coffey (Senior Application Scientist, Agilent Technologies)</p><p>Andrew Coffey joined Agilent Technologies Biocolumns Applications Team in 2011. &nbsp;He has over 35 years experience in method development, technical support and troubleshooting of reversed phase, ion exchange, size exclusion and many other liquid chromatographic techniques.</p></blockquote>

Agilent Technologies: Inert Separations for Oligo LC-MS Workflows

Inert Separations for Oligo LC-MS Workflows

Explore Agilent’s new HPLC columns in a webinar on advanced oligonucleotide separations, sequencing, and impurity profiling.