At UC San Diego, researchers are using patient-derived brain organoids and spaceflight to explore whether aging-associated biology can be accelerated in orbit. With advanced proteomics enabled by the Thermo Scientific Orbitrap Astral Mass Spectrometer (MS), the team is gaining deeper insight into complex brain biology and new possibilities for studying neurodegenerative disease.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Neuroscience_Takes_Flight_How_Collaboration_Spaceflight_and_Orbitrap_Astral_MS_Are_Opening_a_New_Window_into_Brain_Aging_6b56e04145.webp" alt="Thermo Fisher Scientific: Neuroscience Takes Flight: How Collaboration, Spaceflight, and Orbitrap Astral MS Are Opening a New Window into Brain Aging" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Neuroscience_Takes_Flight_How_Collaboration_Spaceflight_and_Orbitrap_Astral_MS_Are_Opening_a_New_Window_into_Brain_Aging_6b56e04145.webp 245w," sizes="100vw" width="1024" height="512"><h3>Introduction</h3>Neurodegenerative diseases remain among the most difficult conditions to study. Researchers cannot easily or safely biopsy living brain tissue, and even sophisticated disease models can take years to reveal meaningful biological change. At UC San Diego, Drs. Aline Martins and Alysson Muotri are taking a different approach: creating patient-derived brain organoids from skin cells and sending them to space to explore whether low Earth orbit can accelerate aging-associated biology. Preliminary data suggest that 30 days in space may compress roughly 10 years of aging biology into a much shorter experimental window, opening the door to a faster path for understanding neurodegeneration and evaluating new therapeutic ideas.<h3>A study design built around biology</h3>The real power of this work begins with the study design. Rather than relying on generalized models, the researchers begin with skin cells from patients and generate 3D mini-brain organoids that can model diseases such as Alzheimer’s while preserving critical patient context. That gives the team a more human-relevant system for studying how aging and disease unfold over time. Spaceflight adds a second dimension: a research environment that is exceptionally difficult to reproduce on Earth, but one that may help reveal the molecular and cellular drivers of neurodegeneration faster than conventional studies allow. The Integrated Space Stem Cell Orbital Research (ISSCOR) Center at UC San Diego have begun to explore this broader approach to study how space-related stressors influence brain aging and cognitive decline, with potential relevance to diseases such as Alzheimer’s and dementia. Once those organoids return to Earth, the next challenge is extracting as much insight as possible from every sample.<h3>Enabling deeper insight from limited samples</h3>Brain organoids are inherently heterogeneous, with multiple cell subtypes that may respond differently to aging and environmental stress.To extract deeper biological insight from the organoid samples, the researchers describe the<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/836"> Orbitrap Astral MS </a>as central to their investigation. They highlight that the high sensitivity required for single-cell proteomics is essential for revealing how the space environment affects distinct cell populations and the Orbitrap Astral MS helps unlock deeper biological insight.That distinction is important: the value of the Orbitrap Astral MS analytical workflow is not simply generating more data but importantly helping the team resolve more of the biology within a complex model.<h3>How Orbitrap Astral MS helps push biology further</h3>At Thermo Fisher Scientific, we work alongside researchers pursuing bold scientific questions by developing technologies that help them see deeper into complex biology.Aline describes Orbitrap Astral MS as a major advance for the sensitivity and resolution this research requires. Its synchronized data acquisition capabilities and parallel ion processing across dual high-resolution accurate-mass analyzers with maximized instrument utilization help deliver deeper coverage, higher sensitivity, faster throughput, and accurate, precise quantitation across demanding biological workflows.For studies like this one, those capabilities help researchers get more from limited sample material, uncover subtle disease-relevant changes, and translate an inventive experimental design into deeper biological insight, faster.<h3>Looking ahead</h3>The power of this research lies in the convergence of ideas. Patient-derived brain organoids offer a human-relevant model of disease. Spaceflight may serve as an aging accelerator. Advanced proteomics helps researchers understand what complex biological models are revealing with greater depth and clarity, while single-cell proteomics unlocks a view into cell-specific changes that can drive disease biology. Together, this approach could give scientists a faster and more informative way to study late-onset diseases such as Alzheimer’s and dementia. This initiative between UC San Diego and Thermo Fisher Scientific shows how collaborations across the scientific community can open new doors in neuroscience and accelerate discovery in ways that matter. As researchers continue to push the boundaries of what is possible, studies like this one point to a future where deeper biological insight can be reached faster and with greater potential to improve human health.<h3>Learn more</h3><ul><li>Listen to the <a target="_blank" rel="noopener noreferrer" href="https://corporate.thermofisher.com/us/en/index/newsroom/Our-stories/Brains-in-space.html">interview with Dr. Aline Martins</a> for more on the scientific vision behind this research and its implications for brain aging and neurodegenerative disease.</li><li>Learn more about <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/mass-spectrometry/proteomics-mass-spectrometry/single-cell-proteomics.html">Thermo Fisher Scientific single-cell proteomics solutions</a> and how they help researchers gain deeper insight from limited and complex samples.</li><li>Explore the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/836">Thermo Scientific Orbitrap Astral MS</a> and see how it helps researchers achieve deeper biological insight with faster throughput, higher sensitivity, and accurate, precise quantitation across advanced workflows.</li></ul><blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#BrainOrganoids #SpaceBiology #Neurodegeneration #OrbitrapAstralMS</blockquote>

Neuroscience Takes Flight: How Collaboration, Spaceflight, and Orbitrap Astral MS Are Opening a New Window into Brain Aging

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 29th September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you technical note by KNAUER, application note by Shimadzu and posters by Thermo Fisher Scientific / HPLC Symposium and Waters Corporation / AOAC!<h3>1. KNAUER: Efficient separation of di(2-ethylhexyl) phthalate (DEHP) from oil matrices using GPC</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VTN_00_XX_knauer_applichrom_gpc_f1d7f1a236.pdf">Full PDF for download</a></li></ul>Phthalates are chemical compounds primarily used as plasticizers in polymers to enhance the flexibility and elasticity of materials such as polyvinyl chloride (PVC) [1]. Since plasticizers are not covalently bound to the polymer chains, they have the potential to leach into the contacting media and contribute to contamination [2]. With the widespread and increasing use of plastic materials, the risk of phthalate release and human exposure continues to rise. As a result, and due to their potentially harmful effects on human health, phthalates have attracted growing scientific and regulatory attention in recent years [1]. Oil-rich food products can leach lipophilic phthalates from their packaging [3]. Throughout the production and packaging process, polymeric components such as tubing, containers, and caps may serve as potential sources of contamination. For the analysis of phthalates in such oil matrices, sample cleanup is an essential step. Gel permeation chromatography (GPC), based on sizeexclusion principles, is widely applied as an effective cleanup technique prior to subsequent analytical determination.<h4>MATERIALS &amp; METHODS</h4>The samples were dissolved in the eluent mixture and filtered through a 0.45 µm PTFE filter prior to analysis. For both olive oil and canola oil, an additional sample spiked with DEHP was prepared and likewise filtered before analysis. Chromatographic analysis was performed using an isocratic method with an eluent composition of ethyl acetate/heptane (50:50 v/v) at a flow rate of 1.0 mL/min. Detection was carried out by UV absorbance at 254 nm. The injection volume was 8 µL using a full-loop injection mode. The column temperature was maintained at 25°C. Separation was achieved on an AppliChrom® OleoSep column (10 µm, 300 × 8 mm). The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1630">AZURA® Semi Preparative GPC System</a> was used.<h4>CONCLUSION</h4>GPC sample preparation is an effective approach for separating phthalates from high-molecular-weight matrices such as edible oils. In this study, the successful separation of di(2-ethylhexyl) phthalate (DEHP) from three different oil samples was demonstrated. Based on these results, it can be assumed that other phthalates with lower molecular weights (&lt; 390 Da) can also be effectively separated under same conditions. The KNAUER AZURA® GPC semi preparative system (Fig. 4) in combination with the AppliChrom® OleoSep GPC column provides a robust and efficient setup for sample preparation in phthalate analysis, offering rapid separation, high resolution, and minimal solvent consumption due to the short analysis time.<h3>2. Shimadzu: Analysis of Chloride and Sulfate Ions in Bioethanol Using the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+IC">Nexera IC Ion Chromatograph</a></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01211_en_55cbf3e64f.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>Analysis of chloride and sulfate ions in fuel ethanol can be conducted in accordance with ASTM D7319-221) .</li><li>Accurate quantitative determination, exhibiting excellent linearity and repeatability, can be achieved at concentration levels significantly below the limits specified by the ASTM criteria.</li></ul>First-generation bioethanol produced from raw materials such as corn and sugarcane has been utilized to achieve carbon neutrality. However, research and development for second-generation bioethanol produced from non-food, cellulose-based raw materials is also underway to avoid competition with food crops. In Japan, eight companies, including Toyota Motor Corporation, have formed the “Research Association of Biomass Innovation for Next Generation Automobile Fuels” (raBit) to advance research on next-generation bioethanol technologies. Shimadzu participates as a supporting member and promotes research and development through analytical technology cooperation.&nbsp;It is known that the presence of chloride and sulfate ions in fuel ethanol can form engine residue, leading to reduced engine performance. ASTM D4806-252) specifies fuel ethanol quality as criterion of chloride ion must not exceed 6.7 mg/kg and that of sulfate ion must not exceed 4 mg/kg. ASTM D7319-22 includes test methods for chloride and sulfate ions in fuel ethanol using ion chromatography.&nbsp;In this application, we present an example of the analysis of chloride and sulfate ions in bioethanol using the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+IC">Nexera IC</a> in accordance with ASTM D7319-22.<h4>Conclusion</h4>Chloride and sulfate ions in bioethanol were determined using a Nexera IC system in accordance with ASTM D7319-22. The method offers high sensitivity, linearity, and repeatability, enabling reliable quantification at concentrations well below ASTM-specified limits, thus supporting effective quality control and regulatory compliance of fuel ethanol.<h3>3. Thermo Fisher Scientific / HPLC Symposium: LC-MS analysis of intact and subunit-level mAb enabled by a novel monodisperse supermacroporous reversed-phase platform</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/po_004763_ccs_surepac_protein_reverse_phase_mabs_separation_po004763_na_en_56199ec50d.pdf">Full PDF for download</a></li></ul>Monoclonal antibodies (mAbs) are structurally complex biotherapeutics that require multi-level analytical characterization to ensure product quality, safety, and efficacy. LC-MS analysis is routinely applied at the intact protein and subunit levels to assess molecular mass, heterogeneity, and structural integrity. Enzymatic subunit generation, such as IdeS digestion, enables targeted domain-level characterization while reducing analytical complexity compared to full peptide mapping. Achieving high-performance reversed-phase separations across intact and subunit-level workflows remains challenging, as chromatographic columns optimized for intact proteins often compromise performance of smaller subunits. Consequently, multiple columns are required to support comprehensive mAb characterization.&nbsp;In this study, we evaluate a reversed-phase separation column based on a novel 2.5 μm monodisperse supermacroporous (SMP) polymeric stationary phase for unified LC-MS analysis of monoclonal antibodies under intact, reduced, and IdeSdigested conditions. The SMP resin’s uniform particle size and highly accessible pore structure enable efficient separation of both small and large analytes, including intact mAbs, reduced subunits (LC and HC), and IdeS-generated fragments such as scFc and F(ab′)₂. Using the SurePac Protein RP MDi column, baseline separation of these species was achieved within a 10- minute gradient, providing a fast, all-in-one LC-MS approach for mAb variant characterization.<h4>Materials and methods</h4><h5>Instruments&nbsp;</h5><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/744">Thermo Scientific Vanquish Horizon UHPLC system</a>; <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/401">Thermo Scientific Orbitrap Exploris 480 mass spectrometer</a> with Thermo Scientific OptaMax NG ion source.&nbsp;<h5>Data analysis&nbsp;</h5>The UHPLC system with mass spectrometer were operated with <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1758">Thermo Scientific Xcalibur 4.7 software</a>. Full MS spectra of intact mAbs and mAb fragments were analyzed using Thermo Scientific Freestyle 1.8 and BioPharma Finder 5.4 software that utilizes the ReSpect and Xtract algorithms for molecular mass determination.<h4>Conclusions&nbsp;</h4><ul><li>A SurePac Protein RP column enabled a rapid (10 min) separation of mAb LC, HC, scFc, and F(ab′)₂ fragments, with major proteoforms identified by mass agreement.&nbsp;</li><li>The SurePac Protein RP column supports comprehensive intact and subunit-level mAb characterization in a single workflow, with strong separation performance across mAb fragments.</li></ul><h3>4. Waters Corporation / AOAC: IMPROVED PURIFICATION OUTCOMES FOR PROBLEMATIC COMPOUNDS USING AN INERT COLUMN WITH POLAR SORBENT</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009616_AOAC_f65ea1b771.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>Whether the goal is to obtain enough intermediate for the next step in a synthetic scheme, to polish a final product, or to create a suitable standard for compound identification, preparative liquid chromatography remains a primary technique for isolating compounds from complex mixtures. Recovering the target compound at the desired purity and yield in the least amount of time is of utmost importance.&nbsp;Polar targets that do not retain, or “sticky” compounds that seemingly disappear on the column are two groups that many consider to be ’problematic’ in purification. “Sticky” compounds, also known as non-specific adsorbers (NSAs), are metal-sensitive targets what can adsorb to positively charged metal surfaces in the LC system flow path and column, making them difficult to detect and collect effectively. Although other methods, such as column pre-conditioning, can be implemented for isolating compounds known to be interactive, this option can be time-consuming and lead to sample loss and excessive solvent waste.&nbsp;Although lab scale purifications in which tens to hundreds of milligrams of target compound are isolated from crude mixtures are still performed routinely, the trend toward smaller scale purification is becoming more prevalent. Laboratories are increasingly opting to isolate only a few milligrams of target compound for preliminary studies. Successful target candidates are then isolated in greater amounts to satisfy ensuing experiments. Whether the objective is to isolate a small amount of target compound for exploratory studies, or to purify greater amounts for their use as reference standards, a column which can resolve eluting impurities efficiently is required.&nbsp;Echinacea is an herbaceous flowering plant in the daisy family1,2 and is one of the most important medical herbs widely used to treat the common cold and other infectious diseases.3 One of the most abundant compounds in echinacea is chicoric acid (Figure 1), a phenylpropanoid and caffeic acid derivative.4,5 In this scale-up study, chicoric acid, a suspected “sticky” target polar compound was isolated from a complex echinacea tea extract using two inert XSelect HSS T3 MaxPeak Premier OBD Columns with different particle sizes and dimensions (3.5 μm 10 x 100 mm and 5 μm 10 x 150 mm). The MaxPeak Premier OBD preparative HPLC columns are considered ’inert’ because they are enabled with Waters High Performance Surface (HPS) Technology. The chicoric acid isolated using the 3.5 μm column was of equivalent UV purity to the product isolated using the 5 μm column, with a 34% savings in run time. Smaller particle preparative isolations using columns with inert hardware are a practical option for those laboratories where reduced amounts of target are needed for projects conducted on accelerated timelines.<h4>METHODS</h4><ul><li>Analytical Columns and flow rate: ACQUITY Premier HSS T3 and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY UPLC HSS T3 Columns</a>; 1.8 μm, 2.1x50 mm; 0.35 mL/min&nbsp;</li><li>Prep Columns and flow rate: XSelect HSS T3 OBD Prep Column and XSelect Premier HSS T3 OBD Prep (custom) Columns, 5 μm, 10x150 mm; XSelect Premier HSS T3 OBD Prep Column, 3.5 μm, 10x100 mm; 5.4 mL/min</li></ul><h5>Instrumentation&nbsp;</h5><ul><li>Analytical: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/198">ACQUITY UPLC H-Class System</a>; Quaternary Solvent Manager, Sample Manager FTN-H, Column Manager, TUV Detector, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1543">ACQUITY QDa Detector&nbsp;</a></li><li>Prep: Waters AutoPurification System; 2545 Binary Gradient Module, 2767 Sample Manager, System Fluidics Organizer, 2998 Photodiode Array Detector</li></ul><h4>CONCLUSIONS&nbsp;</h4><ul><li>Chicoric acid was analyzed and isolated using analytical and preparative MaxPeak Premier HSS T3 Columns with inert surfaces, which increased the peak area and height for improved detection and collection.&nbsp;</li><li>Chicoric acid was successfully isolated using 3.5 and 5 μm XSelect Premier HSS T3 OBD Prep Columns and fraction pool analysis showed equivalent UV purity, which suggests the viability of incorporating small particle preparative chromatography into the purification workflow.&nbsp;</li><li>3.5 μm preparative columns improve purification process efficiency and promote sustainability by reducing method run time, solvent usage, and fraction lyophilization time.&nbsp;</li><li>Inert MaxPeak Premier column hardware, OBD Technology, and small particle preparative columns are well-suited for the demands of the purification laboratory when results must be generated efficiently to satisfy accelerated production timelines</li></ul></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 40, 2026

Pressurized Liquid Extraction: Principle, Method, and Workflow

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Product

Application

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A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

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Trace level analysis like pesticide residue testing comes with specific challenges and performing this analysis in cannabis can be a frustrating experience. Establishing a robust method can help mitigate long term problems but method criteria and performance parameters can be difficult to choose without the benefit of consensus methods and a long history of detailed published methods. It can be hard to know where to start with method fundamentals and how to experimentally determine performance. Luckily, the chemical contaminants community has developed excellent practices that can be used. It is important to understand fundamental criteria and options for determining performance because these directly impact the quality of testing and ability to meet regulatory requirements.
I often help people with pesticide testing in cannabis and will discuss some of the most important method criteria and performance experiments needed when establishing a pesticide method in your lab. This presentation will answer these common questions.
<blockquote>
Presenter: Julie Kowalski, Ph.D. (onsultant at jkSS)
</blockquote>
<blockquote>
Julie Kowalski is a technical consultant primarily serving the cannabis and hemp testing market. She earned her graduate degree in Analytical Chemistry from Pennsylvania State University. Her professional experience includes troubleshooting, method development and validation for GC, GC-MS, LC, and LC-MS/MS in addition to pesticide residue analysis and chromatography method development. She worked at a technology provider for over ten years and recently served as Scientific Director and Chief Scientific Officer for a cannabis testing lab. She has previously served as the President of the North American Chemical Residue Workshop, served on AOAC Expert Review Panels, the Cannabis Scientific Task Force for Washington State and is currently chairing the AOAC CASP Chemical Contaminants Working Group.
</blockquote>

Agilent Technologies: Pesticide Analysis FAQs with Julie Kowalski, Cannabis and Hemp Testing Consultant

Pesticide Analysis FAQs with Julie Kowalski, Cannabis and Hemp Testing Consultant

In this first episode, we’ll take a look at an essential yet very challenging part of the analytical workflow: Sample Preparation. We will explore alternatives to the standard one-variable-at-a-time approach (OVAT) using Design of Experiment (DoE) and we will address how the blissful synergy with automation makes method development not only faster and more time/cost effective, but also capable of finding the right answer across the whole of the experimental space.
In the second half of the webinar, Camilla will be joined by Phil Kay - a learning manager for JMP, a division of SAS Institute. He is among the leading minds in Design of Experiments and has a long history of guiding data analytical solutions for industrial organisations around the world. Phil is a long time collaborator of Anatune so we couldn’t be happier that he’s agreed to be part of the series.
<h3>Method Development With Camilla Liscio</h3>
Method development is the place where analytical science resembles art; sound analytical methods are often rather elegant.
Method development is where analysts use their craft and expertise to find the optimum solution to analytical problems. If it’s a tough one, they may need to think outside the box and find unconventional solutions.
Method development specialists are often the innovators in our industry, who stretch the limits of technology’s current capabilities.
They, more than most, need the self confidence to believe that when they take on a complicated problem, they can see it through, coming up with something that will be fit-for-purpose.
Without this confidence, there is a tendency to copy what others are doing, play it safe and miss the innovations that will get you ahead of the game.
Bearing in mind that once in place, these methods may be there for ten years, it’s certainly worth trying to push the limits of what’s possible.
Unfortunately, the drive toward more specialised job-roles, means most laboratories have just a handful of chemists responsible for all their method development.
These members of staff don’t just need analytical skills, they also need to understand the context of the problem and the regulatory environment.
These skills come with practice and time served. Good people are not easy to find.
What we need is a new generation of specialised method developers.
The people with the ability to train the next generation are often as busy as they are experienced and have little time to transfer their knowledge.
In the opinion of the Community of Analytical Measurement Science, (CAMS UK), the shortage of method development skills is one of the biggest potential threats to the long term health of the analytical sciences.
We want to do our bit, by sharing our hard won expertise, so we have put together a three part webinar series, covering the most important aspects of modern method development.
We’ll look at the three stages of method development: Sample Preparation, Instrumental Analysis and Data Processing.
Camilla Liscio will take you through the fundamentals of each stage, before being joined by an industry specialist to discuss some of their best tips for excellent method development.
<blockquote>
Presenter: Camilla Liscio
</blockquote>
<blockquote>
Camilla is one Anatune’s most experienced and effective method developers. Her background in metrology – the science of measurement and statistics – give her a deep understanding of the mathematics underpinning all of analytical science.
</blockquote>
<blockquote>
This, coupled with her years solving complex analytical problems for LGC and Anatune puts her on extremely strong foundations for method development.
</blockquote>
<blockquote>
If you aspire to become a rising star in your organisation or know anyone earmarked for great work, this is a must attend series.
</blockquote>

Pixabay/michelprado: 1/3 Method Development With Camila Liscio: Sample Prep

1/3 Method Development With Camila Liscio: Sample Prep

Episode 2 of the method development series focusses on instrument analysis, specifically hyphenated techniques (GC-MS and LC-MS). We’ll delve into some of the atypical options for dealing with poor sensitivity and matrix interference.
We’ll be joined by our expert guest Colin Towers – Technical Support Chemist at Crawford Scientific. We’ll discuss the most relevant (sometimes unconventional) tips and tricks to help you add to your method development tool kit.
Colin has decades of experience in solving practical method development problems and is one of an ever decreasing breed of analysts with an intimate knowledge of what’s going on under the hood of your analyser.
<h3>Method Development With Camilla Liscio</h3>
Method development is the place where analytical science resembles art; sound analytical methods are often rather elegant.
Method development is where analysts use their craft and expertise to find the optimum solution to analytical problems. If it’s a tough one, they may need to think outside the box and find unconventional solutions.
Method development specialists are often the innovators in our industry, who stretch the limits of technology’s current capabilities.
They, more than most, need the self confidence to believe that when they take on a complicated problem, they can see it through, coming up with something that will be fit-for-purpose.
Without this confidence, there is a tendency to copy what others are doing, play it safe and miss the innovations that will get you ahead of the game.
Bearing in mind that once in place, these methods may be there for ten years, it’s certainly worth trying to push the limits of what’s possible.
Unfortunately, the drive toward more specialised job-roles, means most laboratories have just a handful of chemists responsible for all their method development.
These members of staff don’t just need analytical skills, they also need to understand the context of the problem and the regulatory environment.
These skills come with practice and time served. Good people are not easy to find.
What we need is a new generation of specialised method developers.
The people with the ability to train the next generation are often as busy as they are experienced and have little time to transfer their knowledge.
In the opinion of the Community of Analytical Measurement Science, (CAMS UK), the shortage of method development skills is one of the biggest potential threats to the long term health of the analytical sciences.
We want to do our bit, by sharing our hard won expertise, so we have put together a three part webinar series, covering the most important aspects of modern method development.
We’ll look at the three stages of method development: Sample Preparation, Instrumental Analysis and Data Processing.
Camilla Liscio will take you through the fundamentals of each stage, before being joined by an industry specialist to discuss some of their best tips for excellent method development.
<blockquote>
Presenter: Camilla Liscio
</blockquote>
<blockquote>
Camilla is one Anatune’s most experienced and effective method developers. Her background in metrology – the science of measurement and statistics – give her a deep understanding of the mathematics underpinning all of analytical science.
</blockquote>
<blockquote>
This, coupled with her years solving complex analytical problems for LGC and Anatune puts her on extremely strong foundations for method development.
</blockquote>
<blockquote>
If you aspire to become a rising star in your organisation or know anyone earmarked for great work, this is a must attend series.
</blockquote>

Pixabay/michelprado: 2/3 Method Development With Camila Liscio: Analysis

2/3 Method Development With Camila Liscio: Analysis

In the third and final episode of the Method Development Series, we will consider Data Processing - the last but by no means least part of the analytical workflow. Software portfolio capabilities have improved tremendously in the past decade, allowing analysts to count on an increasingly diverse toolkit. Camilla is incredibly passionate about this stage of the analytical process so from her perspective, she’s left the best to last.
Camilla will be in discussion with a long time collaborator of Anatune (we’ll reveal who this is, shortly). This discussion promises to provide a diverse and thought-provoking insight into data analysis with one of the best in the business.
<h3>Method Development With Camilla Liscio</h3>
Method development is the place where analytical science resembles art; sound analytical methods are often rather elegant.
Method development is where analysts use their craft and expertise to find the optimum solution to analytical problems. If it’s a tough one, they may need to think outside the box and find unconventional solutions.
Method development specialists are often the innovators in our industry, who stretch the limits of technology’s current capabilities.
They, more than most, need the self confidence to believe that when they take on a complicated problem, they can see it through, coming up with something that will be fit-for-purpose.
Without this confidence, there is a tendency to copy what others are doing, play it safe and miss the innovations that will get you ahead of the game.
Bearing in mind that once in place, these methods may be there for ten years, it’s certainly worth trying to push the limits of what’s possible.
Unfortunately, the drive toward more specialised job-roles, means most laboratories have just a handful of chemists responsible for all their method development.
These members of staff don’t just need analytical skills, they also need to understand the context of the problem and the regulatory environment.
These skills come with practice and time served. Good people are not easy to find.
What we need is a new generation of specialised method developers.
The people with the ability to train the next generation are often as busy as they are experienced and have little time to transfer their knowledge.
In the opinion of the Community of Analytical Measurement Science, (CAMS UK), the shortage of method development skills is one of the biggest potential threats to the long term health of the analytical sciences.
We want to do our bit, by sharing our hard won expertise, so we have put together a three part webinar series, covering the most important aspects of modern method development.
We’ll look at the three stages of method development: Sample Preparation, Instrumental Analysis and Data Processing.
Camilla Liscio will take you through the fundamentals of each stage, before being joined by an industry specialist to discuss some of their best tips for excellent method development.
<blockquote>
Presenter: Camilla Liscio
</blockquote>
<blockquote>
Camilla is one Anatune’s most experienced and effective method developers. Her background in metrology – the science of measurement and statistics – give her a deep understanding of the mathematics underpinning all of analytical science.
</blockquote>
<blockquote>
This, coupled with her years solving complex analytical problems for LGC and Anatune puts her on extremely strong foundations for method development.
</blockquote>
<blockquote>
If you aspire to become a rising star in your organisation or know anyone earmarked for great work, this is a must attend series.
</blockquote>

Pixabay/michelprado: 3/3 Method Development With Camila Liscio: Data Processing

3/3 Method Development With Camila Liscio: Data Processing

Reversed phase is the most common mode of HPLC but if it’s not working for you, don’t despair, there are other options. Perhaps HILIC, Chiral, IEX, GPC/SEC, Affinity, SFC, or HIC might be better suited to your sample type.
In this seminar, we will look at additional chromatographic techniques and discuss:
<ul>
<li>Mechanism of separation
</li>
<li>Column selection and available chemistries
</li>
<li>Typical method and eluent conditions
</li>
</ul>
Becoming familiar with other modes of chromatography and investigating alternative chemistry options can result in an improved separation or provide additional information for your sample.
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

Agilent Technologies: There is More to HPLC Than Reversed Phase; Exploring Other Options

There is More to HPLC Than Reversed Phase; Exploring Other Options

There is More to HPLC: Exploring Other Options

Improper LC connections can create bad chromatography.
In this presentation we will discuss:
<ul>
<li>Types of fittings – what is the best option?
</li>
<li>The importance of using the correct capillary
</li>
<li>How improper connections can affect the chromatogram
</li>
<li>How to make a good connection
</li>
</ul>
Something as simple as a connection can potentially cause chromatography problems that may take you a long time to troubleshoot. Careful attention to making a good connection can reduce frustration and save time troubleshooting.
<blockquote>
Presenter: Golnar Javadi (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.
</blockquote>
<blockquote>
Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.
</blockquote>
<blockquote>
Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.
</blockquote>

Agilent Technologies: All About LC Connections – The Importance of Making a Great One

All About LC Connections – The Importance of Making a Great One

Improper LC connections can create bad chromatography.

LC Connections – The Importance of Making a Great One

Porous particle sizes have decreased to sub-two micron (STM). Separations formerly done on 5 micron particles can now be accomplished on much shorter STM columns in a fraction of the time with equal or better resolution.
For more complex separations, longer columns can generate high plate counts but at much higher pressure. Recently, an alternative particle design, the superficially porous particle (SPP), has developed. The particle concept has been around for over 40 years but the current version consists of a small solid core (e.g., 1.7-µm in diameter) surrounded by a thin porous shell (e.g., 0.5-µm thick) making a 2.7-µm particle diameter.
The efficiency of these SPPs is similar to the STM because of:
<ul>
<li>Consistent spherical shape
</li>
<li>Rapid solute mass transfer
</li>
<li>Narrow particle size distribution
</li>
</ul>
The larger particle size results in a much lower pressure drop when compared to STM particles, making them compatible with older 400 bar instruments as well as UHPLC systems.
This seminar will look at both types of particles and discuss when you might choose one over the other. Examples of pertinent applications will be cited.
<blockquote>
Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.
</blockquote>

Agilent Technologies: Practical Aspects of High Performance Column Selection in LC: Superficially Porous (SPP) or Sub-two Micron (STM)

Practical Aspects of High Performance Column Selection in LC: Superficially Porous (SPP) or Sub-two Micron (STM)

Both types of particles and discuss when you might choose one over the other.

Superficially Porous (SPP) or Sub-two Micron (STM)

Whether your chromatography suddenly changes or you’re not able to reproduce a method from, e.g. a pharmacopeia, isolating the source of the problem can be a challenge.
In this seminar we will develop your troubleshooting skills as we:
Discuss common chromatography problems
<ul>
<li>Pressure
</li>
<li>Peak Shape
</li>
<li>Retention
</li>
<li>Look at how to isolate the problem
</li>
<li>Chemistry related
</li>
<li>Hardware/software
</li>
</ul>
We will also talk about ways to prevent future problems.
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

Agilent Technologies: What's Wrong With My Chromatography? Is it the Column, Method or Instrument

What's Wrong With My Chromatography? Is it the Column, Method or Instrument

Develop your troubleshooting skills. Common chromatography problems.

What's Wrong With My Chromatography?

Wiley&#39;s mass spectral libraries are a powerful tool for the identification of organic compounds in complex mixtures by mass spectrometry.
This series of seminars will demonstrate the use of the NIST (National Institute of Standards and Technology) software suite for accessing Wiley, NIST, and even user-generated libraries. The software includes MS Search, MS Interpreter, and AMDIS plus ChemSketch for structural drawing. The series should be useful to a wide variety of research and developments scientists involved in medical, environmental, industrial, forensic, and biological disciplines.
Initial sessions will primarily discuss electron ionization gas chromatography/mass spectrometry (EI GC/MS) data. However, many of the principles are applicable to tandem spectra obtained by liquid chromatography/mass spectrometry (LC/MS) which will be discussed in the latter part of the series.
<blockquote>
Presenter: James Little (Mass Spectral Interpretation Services)
</blockquote>

Wiley: Spectral Searches with NIST MS Search

Spectral Searches with NIST MS Search

The use of the NIST (National Institute of Standards and Technology) software suite for accessing Wiley, NIST, and even user-generated libraries.