In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with Imad Haidar Ahmad, Scientific Research Director at Amgen leading separations for Discovery Chemistry and Externalization. Imad's career spans Novartis, Merck, and now Amgen, and his research on computer-assisted method development has landed multiple front covers in Analytical Chemistry.We dig into how his group builds retention models from as few as nine lab experiments to map an entire separation landscape — replacing weeks of trial and error with a resolution map you can query and rebuild from. Imad breaks down where this modeling approach struggles (large molecules, SFC, fully autonomous method development), walks through two-dimensional liquid chromatography in plain terms for anyone who's only worked in 1D, and shares the story behind his group's greenness-score mapping work — including where swapping acetonitrile for methanol worked, and where it didn't.We also talk career advice: what a typical day looks like leading a discovery-stage separations team, how undergrad and grad students can bridge the academia-to-industry gap, and what Imad sees as the field's biggest unsolved problem.<h3>Video Transcription</h3>Chromatographic method development is increasingly moving beyond trial and error toward workflows built on modeling, automation, multidimensional separations, and smarter use of data. In a recent interview, Haidar Ahmad discussed how these approaches are changing pharmaceutical analysis, why understanding the fundamentals of chromatography remains essential, and where some of the biggest opportunities in separation science still lie.Ahmad’s route into analytical chemistry was not entirely planned. As an undergraduate, he initially found inorganic and organic chemistry particularly attractive, but a final-year project focused on the analysis of additives in bread introduced him to the possibilities of analytical chemistry. During his PhD, his research moved into polymer analysis and structure–property relationships. His interest in chromatography deepened when he encountered two-dimensional liquid chromatography (2D-LC), particularly its ability to reveal forms of polymer heterogeneity that could not be adequately characterized using a single separation technique. This eventually led to postdoctoral work with Peter Carr, where Ahmad focused on chromatographic separations and the fundamentals of chromatography.His subsequent career in the pharmaceutical industry exposed him to different stages of drug development, from late-stage method development and validation to drug substance characterization, purification, and finally discovery-stage separations. That breadth of experience has shaped his view of chromatography as both a scientific discipline and a practical problem-solving tool. In late-stage development, the objective may be a highly robust method capable of successful validation and transfer between laboratories. In discovery, by contrast, speed becomes critical because teams may be working with thousands of molecules while trying to identify the best candidates to advance.&nbsp;<h4>Discovery-stage chromatography: speed, prioritization, and throughput</h4>For a separations team supporting drug discovery, the working day is shaped by a constant flow of requests from medicinal chemistry and other partner groups. The challenge is not simply to perform the analyses, but to decide which requests are most urgent, remove bottlenecks, communicate with project teams, and continuously improve existing workflows.According to Ahmad, one of the most important questions is whether a task can be done faster or in a more high-throughput way. Discovery laboratories operate under strong time pressure, and efficiency therefore becomes part of the scientific strategy rather than merely an operational concern. As he describes it, the work combines science, prioritization, leadership, and communication, all with the aim of moving the most important projects forward as quickly as possible.<h4>In silico modeling: letting the computer perform the experiments</h4>One of the central themes of Ahmad’s work is in silico chromatographic modeling. In practical terms, the approach begins with a carefully designed set of laboratory experiments—often around nine runs. The resulting data are fed into software that builds retention models describing how analyte retention changes with variables such as mobile-phase composition and temperature.The software can then generate a resolution map representing a much larger separation space than could realistically be tested experimentally. Instead of running dozens or hundreds of conditions by trial and error, the analyst can identify regions where critical peak pairs are predicted to achieve the best separation.For Ahmad, the value of these maps goes beyond simply finding the condition with the highest resolution. Each point on the map represents a chromatographic condition, while the visual output shows where poor and strong separations are expected. A broad region of good resolution is particularly useful because it points toward a more robust operating space rather than a narrowly defined optimum that might be sensitive to small variations in experimental conditions.He therefore prefers to think of a chromatographic model as a map or database, rather than simply an optimization tool. If an impurity later disappears from the process, the corresponding peak can be removed from the model and new conditions selected without repeating the complete experimental study. Conversely, if a new degradation product appears during stability studies, the peak can be added and the existing model used to search for updated separation conditions.<h4>Why trial and error still has a place</h4>Modeling does not mean that conventional trial-and-error method development has disappeared. Ahmad points out that it remains useful in early discovery, particularly for fit-for-purpose methods where speed is more important than achieving a fully optimized separation.The situation changes later in development. A method intended for validation, transfer, or long-term routine use carries much greater risk. Trial and error may identify a condition that appears satisfactory without revealing whether it represents the most robust region of the separation space. If that method later fails during transfer or routine operation, the consequences can be costly.For late-stage methods, the additional investment required to build a model can therefore reduce risk over the much longer lifetime of the analytical procedure.<h4>Models are only as good as the experiments behind them</h4>Chromatographic modeling is not automatic proof against poor method development. Ahmad compares the technology to a GPS: it is highly useful, but only if the analyst understands where they are trying to go.Careful experimental design remains essential. Incorrect dwell-volume or column-volume information, as well as insufficient column re-equilibration, can produce misleading predictions. Importantly, these problems may not be obvious when the experimental runs used to construct the model are compared with their calculated values. They may emerge only when the software begins predicting new conditions within the modeled design space.Modeling is also easier for some separations than others. Ahmad describes reversed-phase LC of small molecules as comparatively straightforward, while large biomolecules are more challenging because their retention can be highly sensitive to small changes in mobile-phase composition and temperature.Other separation modes present additional difficulties. Ahmad notes that his teams have found supercritical fluid chromatography (SFC) particularly challenging to model successfully. Fully autonomous method development remains another unresolved goal: current tools can automate parts of optimization, but analyst input is still required.<h4>Why two dimensions can reveal what one dimension cannot</h4>Ahmad’s early interest in 2D-LC came from polymer characterization. He explains the principle simply: one-dimensional chromatography may answer one question about a sample, while two complementary dimensions can answer two.Two copolymers, for example, may have similar molecular size and therefore coelute in size-exclusion chromatography. A second separation mechanism based on interaction with a stationary phase may distinguish them according to chemical composition. Combining both dimensions provides a more complete description of complex distributions involving molecular weight, composition, sequence, and other forms of heterogeneity.Historically, the complexity of both instrumentation and software limited wider adoption of 2D-LC. Ahmad recalls systems requiring several computers and multiple software packages. That situation has improved substantially, and 2D-LC is now easier to implement across research and pharmaceutical development.Nevertheless, modeling multidimensional separations still presents challenges. While models can be built for individual fractions or dimensions, current software does not easily identify a common optimum across multiple models simultaneously. Much of this comparison still requires manual work.<h4>Bringing sustainability into method development</h4>Another area where Ahmad sees room for change is green analytical chemistry.Traditional pharmaceutical method development has understandably prioritized resolution, sensitivity, robustness, and turnaround time. Sustainability has often been treated as a secondary consideration, particularly at the analytical scale, where solvent use appears small compared with preparative chromatography or manufacturing.That mindset is beginning to change. Ahmad argues that greenness metrics should increasingly be incorporated directly into method-development software. If analysts could immediately see how changes in flow rate, gradient conditions, solvent choice, or other parameters affect the environmental profile of a method, sustainability could become part of routine optimization rather than an assessment performed after the method has already been developed.The objective, however, is not to pursue the greenest possible method regardless of analytical performance. Ahmad emphasizes the need to develop methods that are as green as possible while remaining fit for purpose. Modeling can potentially help balance these competing requirements by identifying conditions that preserve separation performance while allowing greener solvents or alternative chromatographic conditions to be introduced.<h4>Advice for young chromatographers: do not treat HPLC as a black box</h4>For students learning chromatography, Ahmad’s central recommendation is straightforward: do not treat the HPLC system as a black box.Rather than collecting chromatograms by making random changes, he encourages analysts to build a mental model of the separation. When temperature, gradient, or another parameter is changed, they should ask why retention or selectivity changed and use each experiment to improve their understanding of the system.He also recommends making use of educational resources on chromatographic fundamentals and method development and, importantly, not being afraid to experiment with instrument conditions. Modern systems are robust, but the analyst still needs to understand the underlying separation science in order to use them effectively.The same proactive attitude applies to career development. Ahmad strongly encourages students to seek industry internships and make connections beyond their own institutions. Conferences and poster sessions can provide natural opportunities to meet scientists from industry, discuss research, and ask about internship opportunities. Based on his own experience working with undergraduate and graduate interns, these collaborations can benefit both students and research teams and may strongly influence later academic or career decisions.<h4>What still needs to be solved?</h4>Asked about the biggest unresolved challenges in separation science, Ahmad does not begin with technology. Instead, he points to a shortage of well-trained analytical chemists with a strong understanding of chromatographic fundamentals.Alongside education, he identifies several technical opportunities. One is truly autonomous method development, where an instrument and software could optimize a separation without continuous analyst input. Another is the ability to predict chromatographic selectivity directly from molecular structure.Such capability could be especially valuable in high-throughput discovery environments. If a laboratory could predict which stationary phase and conditions were most likely to separate a particular set of compounds or isomers before entering the laboratory, method development could become considerably faster and more efficient.Taken together, Ahmad’s perspective points toward a future in which chromatography becomes increasingly model-driven, automated, multidimensional, and sustainability-aware. But the technology does not eliminate the need for chromatographic knowledge. On the contrary, as analytical tools become more powerful, understanding the fundamentals may become even more important—the software can explore the separation space, but the scientist still needs to know what question to ask and how to interpret the answer.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 In Silico Modeling, 2D-LC & Green Chromatography: Imad Ahmad on the Future of Method Development

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

Researchers from CATRIN at Palacký University, together with colleagues from VSB – Technical University of Ostrava and Charles University, have developed a new graphene ink for producing inexpensive and sensitive printed sensors. Using single-atom engineering, they incorporated copper atoms into the material, enabling highly sensitive detection of dopamine – a neurotransmitter responsible for transmitting signals between nerve cells. The approach, <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">presented in the journal Advanced Science</a>, has strong potential for use in medical diagnostics.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp 117w," sizes="100vw" width="1920" height="2560">Single-atom engineering makes it possible to control the chemical reactivity of materials at the level of individual atoms. Its broader application in medical diagnostics has so far been limited by a lack of atomically engineered materials and printable inks based on them. Researchers have now developed a new class of single-atom-doped graphene materials as well as an ink for producing sensitive printed sensors. Copper proved particularly effective for dopamine detection, which is important for biomedical research and the development of advanced diagnostic technologies for diseases including Alzheimer’s disease.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp 117w," sizes="100vw" width="1920" height="2560">“The NGA-Cu ink we developed enables the production of fully inkjet-printed, sustainable paper-based electrodes at very low cost. Each sensor requires only 1.3 micrograms of active ink, costs approximately one Czech koruna and provided a stable response for at least three months. The ink is also fully compatible with commonly available materials inkjet printers. This creates significant potential for producing affordable, digitally designed sensors whose function can be tailored atom by atom,” said&nbsp;the corresponding author of the <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">paper</a> Michal Otyepka.The basis for this new generation of single-atom inks was nitrogen-doped graphene acid (NGA), which CATRIN researchers developed several years ago. They have now progressively enriched it with different individual metal atoms, including copper, iron and manganese.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp 117w," sizes="100vw" width="1920" height="2560">“We found that the electrochemical response depends fundamentally on the type of atom used. While copper atoms significantly enhance the dopamine signal, other metals suppress it. What matters, therefore, is not only the presence of isolated metal atoms but also the specific metal involved. This is a very important finding for the further development of biosensors,” explained first author Martin-Alex Nalepa.The published results introduce a new class of sensing materials with considerable potential for medical diagnostics. The researchers present a broadly applicable platform for developing graphene inks containing individual metal atoms and open the way towards scalable, reproducible and material-efficient production of advanced electrochemical sensors.&nbsp;“We are only at the beginning and are fascinated by the new possibilities opening up before us. We can see that by changing the type of atom, we can control the sensitivity and selectivity of the sensor. In addition to dopamine, we can also determine immunoglobulins very precisely – antibodies whose levels and types may serve as important indicators of autoimmune or infectious diseases,” emphasised Radek Zbořil, a co-author of the paper.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp 117w," sizes="100vw" width="1920" height="2560">Electrochemical biosensors can find applications not only in medicine but also in food quality control, drinking-water monitoring and environmental pollution monitoring. Their key components are functionalised electrodes, and graphene-based materials have proved particularly suitable for their production. A key factor in the success of the Olomouc researchers was the targeted preparation of graphene ink with the required properties.The research was supported by the TECHSCALE project under the Johannes Amos Comenius Programme.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics: Project TECHSCALE (No. CZ.02.01.01/00/22_008/0004587) is financed by the MEYS OP JAC Excellent research programme, supported by ERDF/ESF." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg 245w," sizes="100vw" width="620" height="87"><blockquote><h4>Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">Single-Atom-Enhanced Fully Inkjet-Printed Electrochemical Sensor for Dopamine Detection</a></h4>Martin-Alex Nalepa, David Panáček, Vítězslav Hrubý, Matěj Jendřišák, Rostislav Langer, Michal Langer, Petr Jakubec, Ivan Dědek, Vojtěch Kupka, Michal Mazur, Radek Zbořil, Michal OtyepkaAdv. Sci., 2026, e76796<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/advs.76796">https://doi.org/10.1002/advs.76796</a><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Single-atom (SA) engineering offers atomic-level control over interfacial reactivity, yet the lack of printable SA-based inks hinders its practical translation into electrochemical sensing. Here, a fully water-based inkjet-printable ink is introduced based on nitrogen-doped graphene acid (NGA) hosting atomically dispersed Cu centers (NGA-Cu-ink). The ink enables digitally controlled, spatially defined deposition and fabrication of low-cost ($0.04 per sensor), fully inkjet-printed sustainable electrodes on paper. A comparison of NGA functionalized with different SA dopants (Cu, Mn, Fe, Ce) identifies a strong dopant-dependent electrochemical response, with Cu uniquely enhancing the analyte signal while other dopants suppress it, demonstrating that SA identity is a decisive design parameter in printed sensing interfaces profiling sensitivity and selectivity. The functional role of the NGA support is to provide dense anchoring sites (nitrogen and carboxylate groups) that stabilize atomically dispersed metal centers and create adsorption- and electron-transfer–active microenvironments. The NGA-Cu-ink yields enhanced dopamine oxidation, enabling quantitative detection on fully printed devices (limit of detection 9.7 µM; linear range 50–400 µM) and printed-on-electrode platforms (10.6 µM), while maintaining &lt;10% signal variation over 11 weeks. The approach establishes a general route to single-atom graphene-based inks for scalable, reproducible, and low-material-consumption manufacturing of advanced electrochemical sensors.

Atom by atom. Scientists reveal a new direction in medical diagnostics

Article

Academy

Scientists

Interview

Science and research

Video

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

The Analytical Scientist

Agilent Technologies

Thermo Fisher Scientific

Restek Corporation

SCIEX

Waters Corporation

As population grows, demand for food consumption and global trade in the food industry has also increased. Hundreds of pesticides are routinely used for crop protection across the globe, traces of pesticides left in treated commodities are called “residues”.
Regulation (EC) No 396/2005 sets maximum residue levels of pesticides in or on food and feed and is under constant revision as shown by the recent amendment EU 2021/155, published February 9th 2021. A growing target list of pesticides in complex matrices, and the need for low limits of detection, bring various challenges to testing laboratories.
<h3>Pesticide Thursdays - A Waters&#39; 3-episode webinar series</h3>
<h4>Episode 3 - Polar Pesticides analysis in challenging food matrices</h4>
Routine analysis of anionic polar pesticides is challenging but has become a requirement for many food laboratories. With the move to a generic and simplified extraction techniques, the demand for lower limits of quantification for these negative ionization analytes has been developed.
In this webinar, our aim is to focus on routine analysis of anionic polar pesticides in complex matrices such as flour.
Join us for the Pesticide Thursdays webinar series and learn more about Waters turn-key solutions developed to tackle pesticide residue analysis in food and feed.
We are looking forward to discussing these topics with you.
Your Analytical Foodies Team
<blockquote>
Presenter: Benjamin Wuyts (Senior Solution Support Specialist, Waters Corporation)
</blockquote>
<blockquote>
Benjamin initially joined Waters as an application chemist working in the Belgian demo lab, performing liquid chromatography-mass spectrometry demonstrations, support and training. In 2016 Benjamin became a European application chemist focusing on projects to support emerging applications. Benjamin has been focused over several years in establishing the Waters solution to the analysis of polar anionic pesticides, utilising novel column phases and LC-MS/MS technologies.
</blockquote>

Waters Corporation: Polar Pesticides analysis in challenging food matrices - Pesticide Thursdays Webinar Series

Polar Pesticides analysis in challenging food matrices - Pesticide Thursdays Webinar Series

In this webinar, our aim is to focus on routine analysis of anionic polar pesticides in complex matrices such as flour.

Polar Pesticides analysis in challenging food matrices

Karl Fischer Titration is a standard analytical method that is applied in many laboratories around the world for precise and fast water determination. However, its precision strongly depends on the correct procedure – in this webinar you will learn the most important steps to consider:
1) Basics of Karl Fischer Titration
2) What is the most suitable Karl Fischer titration method for my sample or sample matrix?
3) Correct titer determination and why is it important?
4) Application development
5) Troubleshooting
This webinar is intended for everybody who is interested in learning more details about Karl Fischer Titration, developing Karl Fischer applications, or working in QC/QA departments, especially lab technicians, lab managers and quality assurance managers.
<blockquote>
Presenter: Michael Margreth (Application Specialist, Metrohm)
</blockquote>
<blockquote>
Michael Margreth studied chemistry at the University of Applied Science in Muttenz, Switzerland. He joined Metrohm in 2005 as an application specialist and gained titration experience in the application laboratory.
</blockquote>
<blockquote>
Since 2008 he mainly focuses on Karl Fischer titration and the determination of water.
</blockquote>
<blockquote>
Presenter: Bettina Jubb Straub (Global Product Manager, Merck KGaA)
</blockquote>
<blockquote>
Bettina is the global product manager and lead expert for titration at Merck KGaA based in Darmstadt, Germany.
</blockquote>
<blockquote>
She has more than 20 years’ experience in the instrumental analysis business, especially with Karl Fischer Titration and general Titration, Bettina is a graduated engineer and holds a degree in Chemical Technology from the University of Applied Sciences, Darmstadt Germany and a degree as Medical Technical Laboratory Assistant from the Medical Technical Academy Esslingen, Germany.
</blockquote>

The Analytical Scientist: Karl Fischer Titration – A step-by-step guide to correct results

Karl Fischer Titration – A step-by-step guide to correct results

Karl Fischer Titration precision strongly depends on the correct procedure – in this webinar you will learn the most important steps to consider.

Karl Fischer Titration - A step-by-step guide

During this webinar we will take a look at some of the latest trends and hot topics that are being observed within regulated laboratories. We will also discuss various processes that you need to implement in order to remain compliant with the latest regulatory guidance and minimize your inspection risk.
<h5>Specific topics that will be covered will include:</h5>
<ul>
<li>Custom Analytical Instrument Qualification (AIQ) based on User Requirement Specifications (URS)
</li>
<li>Repair Qualification (RQ)
</li>
<li>Hidden costs of Self-Qualification
</li>
<li>Computer System Validation (CSV)
</li>
</ul>
<blockquote>
Presenter: Garry Wright (European Laboratory Compliance Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Garry Wright is the European Laboratory Compliance Specialist for Agilent Technologies and a member of the Agilent Compliance Council. Garry worked in Regulated Pharmaceutical industry for 20 years prior to joining Agilent.
</blockquote>
<blockquote>
His industrial experience focussed on development and implementation of Quality Management Systems, GMP training and Compliance Auditing. Garry’s role within Agilent is to provide Compliance Consulting services to Agilent and our customer network.
</blockquote>
<blockquote>
Garry has presented topics relating to Regulatory Compliance, Data Integrity and Instrument Qualification at a variety of Compliance forums over the last 7 years since joining Agilent.
</blockquote>

Agilent Technologies: How to Minimize Compliance Risk in Regulated Laboratories

How to Minimize Compliance Risk in Regulated Laboratories

During this webinar we will take a look at some of the latest trends and hot topics that are being observed within regulated laboratories.

The HPLC instrument has been at the heart of many analytical laboratories for several decades now. Quite often analysts may be introduced to the concept of chromatography through the course of study, but it’s much less likely that the mechanics of the system will have been explored in detail. Because of this, it can be a bit daunting approaching problem solving with these instruments.
In this presentation, we’ll be walking you through an overview of the processes involved with basic HPLC instrumentation. From there we’ll examine in a greater depth how each component of a HPLC system comes together to provide the analysis you may be looking for, looking at the finer mechanical details of what is taking place, and offering tips along the way to keep your system running smoother for longer.
<h5>This webinar aims to:</h5>
<ul>
<li>Provide a general overview of what an HPLC Instrument is</li>
<li>Look in greater depth at individual sections of the HPLC instrument to describe how they function</li>
<li>Offer some advice on how to look after your instrument to keep it running well for longer</li>
</ul>

Restek: LC Hardware Troubleshooting part 1

LC Hardware Troubleshooting part 1

In this presentation, we’ll be walking you through an overview of the processes involved with basic HPLC instrumentation.

Welcome to the school of high-throughput mass spectrometry where you will learn how to take your lab to new heights. If you are like many who are struggling to meet the challenges of increased sample volumes with shorter turnaround times you have come to the right place. Our goal is to ensure that you have the best tools possible to meet these challenges and do more with less. Do you have the need for speed?
RapidFire takes throughput to the extreme by eliminating chromatography from the equation. Discover how you can supercharge high quality mass spec data with online solid phase extraction. From pharmaceutical research to life sciences and beyond, get answers in just seconds per sample.
<blockquote>
Presenter: KJennifer Hitchcock (LCMS Applications Scientist, Agilent Technologies Inc.)
</blockquote>
<blockquote>
Jennifer is an Application Scientist with Agilent Technologies, developing methods utilizing LC and RapidFire front ends with mass spec detection for clinical and cannabis labs. Prior to joining Agilent in 2015, Jennifer worked as an R&amp;D scientist in a clinical testing lab in North Carolina. In her spare time, Jennifer enjoys spending time with her family in Richmond, Virginia.
</blockquote>

Agilent Technologies: Going Ballistic: Using RapidFire to Reach New Heights in Lab Productivity

Going Ballistic: Using RapidFire to Reach New Heights in Lab Productivity

Discover how you can supercharge high quality mass spec data with online solid phase extraction. 

Using RapidFire to Reach New Heights in Lab Productivity

SCIEX has been at the forefront of bringing innovative MS technologies to its customers and creating platforms to share the expertise of our customers. SCIEX aims to make you more productive with our end-to-end LC-MS/MS solutions and this November we are organizing a virtual Pharma Conclave.
<h5>Who should attend:</h5>
Individuals with an interest in
<ul>
<li>Impurity profiling strategies for APIs and drug products</li>
<li>Pharmaceutical – regulations, methods, and challenges</li>
<li>E&amp;L testing and regulations</li>
<li>Cleaning validation</li>
<li>Pharma regulatory landscape in India and the global context</li>
</ul>
<h5>Agenda: Day 1 - November 24, 2022</h5>
<ul>
<li>10.30 - 10.35 a.m. Introduction to SCIEX </li>
<li>10.35 - 11.00 a.m. Nitroso impurities - current regulatory updates, challenges, and suggested solutions</li>
<li>11:00 - 11:25 a.m. Nitrosamines, NDSRIs and azido impurities as cohorts of concern in pharmaceuticals</li>
<li>11:25 - 11.50 a.m. Challenges in quantifying NDSRIs in pharmaceutical drug products</li>
<li>11.50 - 12.30 p.m. Panel discussion on nitroso impurities - need gap analysis and directions for the Indian pharma industry</li>
</ul>
 
Presenter: Dr. BM Rao (Partner &amp; CEO at QDOT Associates)
Presenter: Dr. Saranjit Singh (Ex-Dean and HOD, Dept. of Pharmaceutical Analysis, NIPER, Mohali)
Presenter: Dr. Harsha K N (Head - Analytical Characterization at Shilpa Medicare LTD, Dabaspet, Bangalore)
Presenter: Shivkumar Vishwanathan (Director and V.P Marketing at GVS Cibatech Pvt Ltd, Mumbai)
Presenter: Dr. Dama Venugopal Rao (Lead-Structural Characterisation and Analytical Expert- complex injectables at Dr. Reddy&#39;s Laboratories)

SCIEX: SCIEX Pharma Conclave 2022 (Day 1)

SCIEX Pharma Conclave 2022 (Day 1)

SCIEX aims to make you more productive with our end-to-end LC-MS/MS solutions and this November we are organizing a virtual Pharma Conclave.

SCIEX has been at the forefront of bringing innovative MS technologies to its customers and creating platforms to share the expertise of our customers. SCIEX aims to make you more productive with our end-to-end LC-MS/MS solutions and this November we are organizing a virtual Pharma Conclave.
<h5>Who should attend:</h5>
Individuals with an interest in
<ul>
<li>Impurity profiling strategies for APIs and drug products</li>
<li>Pharmaceutical – regulations, methods, and challenges</li>
<li>E&amp;L testing and regulations</li>
<li>Cleaning validation</li>
<li>Pharma regulatory landscape in India and the global context</li>
</ul>
<h5>Agenda: Day 1 - November 24, 2022</h5>
<ul>
<li>10.30 - 10.35 a.m. Welcome note</li>
<li>10.35 - 11.00 a.m. Regulatory landscape for extractables and leachables testing</li>
<li>11:00 - 11:25 a.m. Challenges in extractables and leachables (E&amp;L) study in pharmaceutical</li>
<li>11:25 - 11.50 a.m. Challenges in equipment cleaning and contamination control of nitrosamines</li>
<li>11.50 - 12.30 p.m. Panel discussion on E&amp;L regulatory - landscape, guidelines on pharmaceutical packaging systems</li>
</ul>
Presenter: Dr. BM Rao (Partner &amp; CEO at QDOT Associates)
Presenter: Dr. Saranjit Singh (Ex-Dean and HOD, Dept. of Pharmaceutical Analysis, NIPER, Mohali)
Presenter: Dr. Harsha K N (Head - Analytical Characterization at Shilpa Medicare LTD, Dabaspet, Bangalore)
Presenter: Shivkumar Vishwanathan (Director and V.P Marketing at GVS Cibatech Pvt Ltd, Mumbai)
Presenter: Dr. Dama Venugopal Rao (Lead-Structural Characterisation and Analytical Expert- complex injectables at Dr. Reddy&#39;s Laboratories)

SCIEX: SCIEX Pharma Conclave 2022 (Day 2)

SCIEX Pharma Conclave 2022 (Day 2)

In today’s market, obtaining low interest rates has become increasingly challenging. But what if there is still a way to obtain the equipment you need easily and affordably?
Find out in this webinar how our Financial Solutions Team could help your business and take advantage of this limited-time, competitive rate offer on all our manufactured products and services. Count on us to provide personalized and flexible financial solutions to help you overcome budgetary constraints, meet your business goals, and drive innovation. Our Financial Solutions Team will partner with you to understand your unique requirements and develop cost-effective financial solutions that will help you maximize your resources and obtain the latest technology when you need it. 
Join this webinar and discover innovative equipment financing solutions that support your business goals and budget. 
<h5>Learning points:</h5>
<ul>
<li>Learn how financial and leasing services can help to finance your new lab equipment </li>
<li>Learn about innovative equipment financing solutions that support your business goals and budget </li>
<li>Learn how our financial solutions can help you obtain the equipment you need easily and affordably</li>
</ul>
 
<h5>Who should attend:</h5>
<ul>
<li>Laboratory leaders and managers who require lab equipment to keep the lab operation and competitive</li>
<li>Laboratory owners who want and need to invest in lab infrastructure </li>
<li>Everyone who is interested in competitive financial solutions for their analytical laboratory</li>
</ul>
 
<blockquote>
Presenter: Roel Philippart (Program Development Manager, Financial Solutions EMEA, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Roel started in 2021 as Program Delelopment Manager at Thermo Fisher Financial Solutions, specifically for the EMEA region.
</blockquote>

Thermo Fisher Scientific: Accelerate discovery, enable success with personalized and flexible financial solutions and market competitive rates

Accelerate discovery, enable success with personalized and flexible financial solutions and market competitive rates

Join this webinar and discover innovative equipment financing solutions that support your business goals and budget. 

Accelerate discovery with flexible financial solutions

The analysis of mineral oil saturated hydrocarbons (MOSH) and mineral oil aromatic hydrocarbons (MOAH) in food samples is a challenging application. Don´t miss this opportunity to ask our experts all your questions regarding the fully automated LC/GC workflow for MOAH/MOAH analysis.
We start with a brief introduction where we talk about upcoming changes in the legal requirements for MOSH/MOAH analysis. The remaining time is fully dedicated to your questions.
There are three ways to submit your questions:
<ul>
<li>Fill out the box on the webinar registration form</li>
<li>For more detailed questions, you can email us at: analyze.eu@thermofisher.com</li>
<li>Ask your questions on the day using the chat function during the live session</li>
</ul>
Of course you are welcome to join even if you don´t have a specific question, so you can pick up useful information and build up expertise.
<blockquote>
Presenter: Jeroen Weyts (SampleQ Analytical Support GC-MS, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Presenter: Jorn Hofsteenge (SampleQ Analytical Support GC)
</blockquote>
<blockquote>
Presenter: Inge de Dobbeleer (Sample Q Account Manager)
</blockquote>
<blockquote>
Presenter: Petra Gerhards (Regional Marketing Manager for GC &amp; GC-MS, EMEA, Thermo Fisher Scientific)
</blockquote>

Thermo Fisher Scientific: Ask-the-experts session on MOSH/MOAH analysis

Ask-the-experts session on MOSH/MOAH analysis

Don´t miss this opportunity to ask our experts all your questions regarding the fully automated LC/GC workflow for MOAH/MOAH analysis.

<h3>Agenda</h3>
<ul>
<li>9:55 Welcome and introduction
</li>
<li>10:00 SCIEX 7500 system hardware overview
</li>
<li>10:30 SCIEX OS software overview — acquisition
</li>
<li>11:00 Lab session part 1 – set up method and data acquisition (pre-recorded)
</li>
<li>11:45 Break
</li>
<li>12:15 Lab session part 2 - data review (pre-recorded)
</li>
<li>13:00 SCIEX OS software overview - data processing
</li>
<li>13:30 Question and answer
</li>
<li>14:00 Closing
</li>
</ul>
<h3>Your hosts for the day</h3>
<h5>Dr Jianru Stahl-Zeng (Sr Technical Marketing Manager)</h5>
<h5>Jack Steed (Technical Specialist)</h5>

SCIEX: SCIEX 7500 System Virtual Tech Day

SCIEX 7500 System Virtual Tech Day

You will learn and experiment from our experts on how to acquire, review and process your data using SCIEX OS software and the SCIEX 7500 system.