In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with Imad Haidar Ahmad, Scientific Research Director at Amgen leading separations for Discovery Chemistry and Externalization. Imad's career spans Novartis, Merck, and now Amgen, and his research on computer-assisted method development has landed multiple front covers in Analytical Chemistry.We dig into how his group builds retention models from as few as nine lab experiments to map an entire separation landscape — replacing weeks of trial and error with a resolution map you can query and rebuild from. Imad breaks down where this modeling approach struggles (large molecules, SFC, fully autonomous method development), walks through two-dimensional liquid chromatography in plain terms for anyone who's only worked in 1D, and shares the story behind his group's greenness-score mapping work — including where swapping acetonitrile for methanol worked, and where it didn't.We also talk career advice: what a typical day looks like leading a discovery-stage separations team, how undergrad and grad students can bridge the academia-to-industry gap, and what Imad sees as the field's biggest unsolved problem.<h3>Video Transcription</h3>Chromatographic method development is increasingly moving beyond trial and error toward workflows built on modeling, automation, multidimensional separations, and smarter use of data. In a recent interview, Haidar Ahmad discussed how these approaches are changing pharmaceutical analysis, why understanding the fundamentals of chromatography remains essential, and where some of the biggest opportunities in separation science still lie.Ahmad’s route into analytical chemistry was not entirely planned. As an undergraduate, he initially found inorganic and organic chemistry particularly attractive, but a final-year project focused on the analysis of additives in bread introduced him to the possibilities of analytical chemistry. During his PhD, his research moved into polymer analysis and structure–property relationships. His interest in chromatography deepened when he encountered two-dimensional liquid chromatography (2D-LC), particularly its ability to reveal forms of polymer heterogeneity that could not be adequately characterized using a single separation technique. This eventually led to postdoctoral work with Peter Carr, where Ahmad focused on chromatographic separations and the fundamentals of chromatography.His subsequent career in the pharmaceutical industry exposed him to different stages of drug development, from late-stage method development and validation to drug substance characterization, purification, and finally discovery-stage separations. That breadth of experience has shaped his view of chromatography as both a scientific discipline and a practical problem-solving tool. In late-stage development, the objective may be a highly robust method capable of successful validation and transfer between laboratories. In discovery, by contrast, speed becomes critical because teams may be working with thousands of molecules while trying to identify the best candidates to advance.&nbsp;<h4>Discovery-stage chromatography: speed, prioritization, and throughput</h4>For a separations team supporting drug discovery, the working day is shaped by a constant flow of requests from medicinal chemistry and other partner groups. The challenge is not simply to perform the analyses, but to decide which requests are most urgent, remove bottlenecks, communicate with project teams, and continuously improve existing workflows.According to Ahmad, one of the most important questions is whether a task can be done faster or in a more high-throughput way. Discovery laboratories operate under strong time pressure, and efficiency therefore becomes part of the scientific strategy rather than merely an operational concern. As he describes it, the work combines science, prioritization, leadership, and communication, all with the aim of moving the most important projects forward as quickly as possible.<h4>In silico modeling: letting the computer perform the experiments</h4>One of the central themes of Ahmad’s work is in silico chromatographic modeling. In practical terms, the approach begins with a carefully designed set of laboratory experiments—often around nine runs. The resulting data are fed into software that builds retention models describing how analyte retention changes with variables such as mobile-phase composition and temperature.The software can then generate a resolution map representing a much larger separation space than could realistically be tested experimentally. Instead of running dozens or hundreds of conditions by trial and error, the analyst can identify regions where critical peak pairs are predicted to achieve the best separation.For Ahmad, the value of these maps goes beyond simply finding the condition with the highest resolution. Each point on the map represents a chromatographic condition, while the visual output shows where poor and strong separations are expected. A broad region of good resolution is particularly useful because it points toward a more robust operating space rather than a narrowly defined optimum that might be sensitive to small variations in experimental conditions.He therefore prefers to think of a chromatographic model as a map or database, rather than simply an optimization tool. If an impurity later disappears from the process, the corresponding peak can be removed from the model and new conditions selected without repeating the complete experimental study. Conversely, if a new degradation product appears during stability studies, the peak can be added and the existing model used to search for updated separation conditions.<h4>Why trial and error still has a place</h4>Modeling does not mean that conventional trial-and-error method development has disappeared. Ahmad points out that it remains useful in early discovery, particularly for fit-for-purpose methods where speed is more important than achieving a fully optimized separation.The situation changes later in development. A method intended for validation, transfer, or long-term routine use carries much greater risk. Trial and error may identify a condition that appears satisfactory without revealing whether it represents the most robust region of the separation space. If that method later fails during transfer or routine operation, the consequences can be costly.For late-stage methods, the additional investment required to build a model can therefore reduce risk over the much longer lifetime of the analytical procedure.<h4>Models are only as good as the experiments behind them</h4>Chromatographic modeling is not automatic proof against poor method development. Ahmad compares the technology to a GPS: it is highly useful, but only if the analyst understands where they are trying to go.Careful experimental design remains essential. Incorrect dwell-volume or column-volume information, as well as insufficient column re-equilibration, can produce misleading predictions. Importantly, these problems may not be obvious when the experimental runs used to construct the model are compared with their calculated values. They may emerge only when the software begins predicting new conditions within the modeled design space.Modeling is also easier for some separations than others. Ahmad describes reversed-phase LC of small molecules as comparatively straightforward, while large biomolecules are more challenging because their retention can be highly sensitive to small changes in mobile-phase composition and temperature.Other separation modes present additional difficulties. Ahmad notes that his teams have found supercritical fluid chromatography (SFC) particularly challenging to model successfully. Fully autonomous method development remains another unresolved goal: current tools can automate parts of optimization, but analyst input is still required.<h4>Why two dimensions can reveal what one dimension cannot</h4>Ahmad’s early interest in 2D-LC came from polymer characterization. He explains the principle simply: one-dimensional chromatography may answer one question about a sample, while two complementary dimensions can answer two.Two copolymers, for example, may have similar molecular size and therefore coelute in size-exclusion chromatography. A second separation mechanism based on interaction with a stationary phase may distinguish them according to chemical composition. Combining both dimensions provides a more complete description of complex distributions involving molecular weight, composition, sequence, and other forms of heterogeneity.Historically, the complexity of both instrumentation and software limited wider adoption of 2D-LC. Ahmad recalls systems requiring several computers and multiple software packages. That situation has improved substantially, and 2D-LC is now easier to implement across research and pharmaceutical development.Nevertheless, modeling multidimensional separations still presents challenges. While models can be built for individual fractions or dimensions, current software does not easily identify a common optimum across multiple models simultaneously. Much of this comparison still requires manual work.<h4>Bringing sustainability into method development</h4>Another area where Ahmad sees room for change is green analytical chemistry.Traditional pharmaceutical method development has understandably prioritized resolution, sensitivity, robustness, and turnaround time. Sustainability has often been treated as a secondary consideration, particularly at the analytical scale, where solvent use appears small compared with preparative chromatography or manufacturing.That mindset is beginning to change. Ahmad argues that greenness metrics should increasingly be incorporated directly into method-development software. If analysts could immediately see how changes in flow rate, gradient conditions, solvent choice, or other parameters affect the environmental profile of a method, sustainability could become part of routine optimization rather than an assessment performed after the method has already been developed.The objective, however, is not to pursue the greenest possible method regardless of analytical performance. Ahmad emphasizes the need to develop methods that are as green as possible while remaining fit for purpose. Modeling can potentially help balance these competing requirements by identifying conditions that preserve separation performance while allowing greener solvents or alternative chromatographic conditions to be introduced.<h4>Advice for young chromatographers: do not treat HPLC as a black box</h4>For students learning chromatography, Ahmad’s central recommendation is straightforward: do not treat the HPLC system as a black box.Rather than collecting chromatograms by making random changes, he encourages analysts to build a mental model of the separation. When temperature, gradient, or another parameter is changed, they should ask why retention or selectivity changed and use each experiment to improve their understanding of the system.He also recommends making use of educational resources on chromatographic fundamentals and method development and, importantly, not being afraid to experiment with instrument conditions. Modern systems are robust, but the analyst still needs to understand the underlying separation science in order to use them effectively.The same proactive attitude applies to career development. Ahmad strongly encourages students to seek industry internships and make connections beyond their own institutions. Conferences and poster sessions can provide natural opportunities to meet scientists from industry, discuss research, and ask about internship opportunities. Based on his own experience working with undergraduate and graduate interns, these collaborations can benefit both students and research teams and may strongly influence later academic or career decisions.<h4>What still needs to be solved?</h4>Asked about the biggest unresolved challenges in separation science, Ahmad does not begin with technology. Instead, he points to a shortage of well-trained analytical chemists with a strong understanding of chromatographic fundamentals.Alongside education, he identifies several technical opportunities. One is truly autonomous method development, where an instrument and software could optimize a separation without continuous analyst input. Another is the ability to predict chromatographic selectivity directly from molecular structure.Such capability could be especially valuable in high-throughput discovery environments. If a laboratory could predict which stationary phase and conditions were most likely to separate a particular set of compounds or isomers before entering the laboratory, method development could become considerably faster and more efficient.Taken together, Ahmad’s perspective points toward a future in which chromatography becomes increasingly model-driven, automated, multidimensional, and sustainability-aware. But the technology does not eliminate the need for chromatographic knowledge. On the contrary, as analytical tools become more powerful, understanding the fundamentals may become even more important—the software can explore the separation space, but the scientist still needs to know what question to ask and how to interpret the answer.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 In Silico Modeling, 2D-LC & Green Chromatography: Imad Ahmad on the Future of Method Development

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

Researchers from CATRIN at Palacký University, together with colleagues from VSB – Technical University of Ostrava and Charles University, have developed a new graphene ink for producing inexpensive and sensitive printed sensors. Using single-atom engineering, they incorporated copper atoms into the material, enabling highly sensitive detection of dopamine – a neurotransmitter responsible for transmitting signals between nerve cells. The approach, <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">presented in the journal Advanced Science</a>, has strong potential for use in medical diagnostics.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp 117w," sizes="100vw" width="1920" height="2560">Single-atom engineering makes it possible to control the chemical reactivity of materials at the level of individual atoms. Its broader application in medical diagnostics has so far been limited by a lack of atomically engineered materials and printable inks based on them. Researchers have now developed a new class of single-atom-doped graphene materials as well as an ink for producing sensitive printed sensors. Copper proved particularly effective for dopamine detection, which is important for biomedical research and the development of advanced diagnostic technologies for diseases including Alzheimer’s disease.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp 117w," sizes="100vw" width="1920" height="2560">“The NGA-Cu ink we developed enables the production of fully inkjet-printed, sustainable paper-based electrodes at very low cost. Each sensor requires only 1.3 micrograms of active ink, costs approximately one Czech koruna and provided a stable response for at least three months. The ink is also fully compatible with commonly available materials inkjet printers. This creates significant potential for producing affordable, digitally designed sensors whose function can be tailored atom by atom,” said&nbsp;the corresponding author of the <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">paper</a> Michal Otyepka.The basis for this new generation of single-atom inks was nitrogen-doped graphene acid (NGA), which CATRIN researchers developed several years ago. They have now progressively enriched it with different individual metal atoms, including copper, iron and manganese.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp 117w," sizes="100vw" width="1920" height="2560">“We found that the electrochemical response depends fundamentally on the type of atom used. While copper atoms significantly enhance the dopamine signal, other metals suppress it. What matters, therefore, is not only the presence of isolated metal atoms but also the specific metal involved. This is a very important finding for the further development of biosensors,” explained first author Martin-Alex Nalepa.The published results introduce a new class of sensing materials with considerable potential for medical diagnostics. The researchers present a broadly applicable platform for developing graphene inks containing individual metal atoms and open the way towards scalable, reproducible and material-efficient production of advanced electrochemical sensors.&nbsp;“We are only at the beginning and are fascinated by the new possibilities opening up before us. We can see that by changing the type of atom, we can control the sensitivity and selectivity of the sensor. In addition to dopamine, we can also determine immunoglobulins very precisely – antibodies whose levels and types may serve as important indicators of autoimmune or infectious diseases,” emphasised Radek Zbořil, a co-author of the paper.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp 117w," sizes="100vw" width="1920" height="2560">Electrochemical biosensors can find applications not only in medicine but also in food quality control, drinking-water monitoring and environmental pollution monitoring. Their key components are functionalised electrodes, and graphene-based materials have proved particularly suitable for their production. A key factor in the success of the Olomouc researchers was the targeted preparation of graphene ink with the required properties.The research was supported by the TECHSCALE project under the Johannes Amos Comenius Programme.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics: Project TECHSCALE (No. CZ.02.01.01/00/22_008/0004587) is financed by the MEYS OP JAC Excellent research programme, supported by ERDF/ESF." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg 245w," sizes="100vw" width="620" height="87"><blockquote><h4>Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">Single-Atom-Enhanced Fully Inkjet-Printed Electrochemical Sensor for Dopamine Detection</a></h4>Martin-Alex Nalepa, David Panáček, Vítězslav Hrubý, Matěj Jendřišák, Rostislav Langer, Michal Langer, Petr Jakubec, Ivan Dědek, Vojtěch Kupka, Michal Mazur, Radek Zbořil, Michal OtyepkaAdv. Sci., 2026, e76796<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/advs.76796">https://doi.org/10.1002/advs.76796</a><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Single-atom (SA) engineering offers atomic-level control over interfacial reactivity, yet the lack of printable SA-based inks hinders its practical translation into electrochemical sensing. Here, a fully water-based inkjet-printable ink is introduced based on nitrogen-doped graphene acid (NGA) hosting atomically dispersed Cu centers (NGA-Cu-ink). The ink enables digitally controlled, spatially defined deposition and fabrication of low-cost ($0.04 per sensor), fully inkjet-printed sustainable electrodes on paper. A comparison of NGA functionalized with different SA dopants (Cu, Mn, Fe, Ce) identifies a strong dopant-dependent electrochemical response, with Cu uniquely enhancing the analyte signal while other dopants suppress it, demonstrating that SA identity is a decisive design parameter in printed sensing interfaces profiling sensitivity and selectivity. The functional role of the NGA support is to provide dense anchoring sites (nitrogen and carboxylate groups) that stabilize atomically dispersed metal centers and create adsorption- and electron-transfer–active microenvironments. The NGA-Cu-ink yields enhanced dopamine oxidation, enabling quantitative detection on fully printed devices (limit of detection 9.7 µM; linear range 50–400 µM) and printed-on-electrode platforms (10.6 µM), while maintaining &lt;10% signal variation over 11 weeks. The approach establishes a general route to single-atom graphene-based inks for scalable, reproducible, and low-material-consumption manufacturing of advanced electrochemical sensors.

Atom by atom. Scientists reveal a new direction in medical diagnostics

Article

Academy

Scientists

Interview

Science and research

Video

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Metrohm

Agilent Technologies

Technology Networks

SelectScience

Waters Corporation

Focus will be given to the safety, authenticity and quality of food and beverages as well as the future of food products such as meat substitutes and alternative proteins.
This online symposium will cover:
<ul>
<li>Untargeted food analysis using different MS platforms</li>
<li>Applications of near-infrared hyperspectral imaging in food quality control</li>
<li>Applications of behavioral science in food research</li>
<li>Solid phase micro-extraction for beverage analysis</li>
<li>Challenges and opportunities in fermentation for production of alternative proteins</li>
<li>ELNs to support data management in food and beverage labs</li>
<li>Key strategies for pesticide analysis using GC-MS</li>
<li>Nutritional and toxic plant-based elemental analysis using ICP-MS</li>
</ul>
<blockquote>
Presenter: Brian Quinn, PhD (Head of Mass Spectrometry, Queen&#39;s University Belfast)
</blockquote>
<blockquote>
Presenter: Vincent Baeten, PhD (Scientific Director, Walloon Agricultural Research Centre)
</blockquote>
<blockquote>
Presenter: Michelle Niedziela, PhD (VP of Research &amp; Innovation, HCD Research)
</blockquote>
<blockquote>
Presenter: Erich Leitner, PhD (Professor, Graz University of Technology)
</blockquote>
<blockquote>
Presenter: Stefan Cretnik, PhD (Product Manager, PAL System)
</blockquote>
<blockquote>
Presenter: Theresa Liao (Content Specialist, SciNote)
</blockquote>
<blockquote>
Presenter: Wade Opperman (Manager of the Americas, SciNote)
</blockquote>
<blockquote>
Presenter: Moran Farhi, PhD (VP of Research, The Better Meat Co.)
</blockquote>
<blockquote>
Presenter: Liyan Xing, PhD (Senior Application Scientist, PerkinElmer)
</blockquote>
<blockquote>
Presenter: Anastasia Andrianova, PhD (GC/MS Applications Scientist, Agilent Technologies)
</blockquote>

Technology Networks: Advances in Food & Beverage Analysis 2023

Advances in Food & Beverage Analysis 2023

The third edition of this online symposium will provide industry and academic scientists with the opportunity to hear from experts from across the field.

High resolution mass spectrometry (HRMS) has gained importance for broad screening in forensic toxicology laboratories with the emergence and constant evolution of novel psychoactive substances.
The Xevo G3 QTof from Waters is the latest generation of high performance QTof technology and includes many enhancements that improve performance and system robustness. 
Among others, ion optics have been improved to enhance the detection of labile drugs such as amphetamines resulting in significantly higher sensitivity. This presentation aims to highlight some of the developments and enhancements that have been made to the recently launched Xevo G3 QTof.
Also detailed in the presentation will be a comparison study conducted between the outgoing Xevo G2-XS QTof with the Xevo G3 QTof, by means of system setup, data generated e.g., richness of information e.g., detection accuracy and analytical sensitivity.
Learning objectives – what you will learn: 
<ul>
<li>Fundamentals of high resolution mass spectrometry</li>
<li>Enhancements made to the recently launched Xevo G3</li>
<li>QTof Comparison data discussion</li>
</ul>
<blockquote>
Presenter: Nayan. S. Mistry (Senior Application Scientist, Forensics, Research &amp; Development, Waters Corporation, Waters Corporation)
</blockquote>
<blockquote>
Nayan Mistry is a Senior Application Scientist at Waters Corporation. Nayan joined Waters in 2009 and is part of a global team dedicated to performing research and development of applications and analytical solutions for Forensic Toxicology. 
</blockquote>
<blockquote>
Nayan’s key focus is on toxicology screening and he has expertise with both nominal mass and accurate mass-based screening using UNIFI and Waters_Connect application software. Nayan plays a key role in the development of these solutions and in the expansion of associated libraries. Currently Nayan is playing a key role in the development and support of the recently launched Xevo G3 QTof mass spectrometer in addition to other responsibilities for the nominal mass applications. His favourite aspect of the role is customer support - Nayan is greatly involved in global on-site customer support and training and has presented his work at conferences worldwide.
</blockquote>

Waters Corporation: High Resolution MSE Toxicology Screening using the Waters Xevo G3 QTof

High Resolution MSE Toxicology Screening using the Waters Xevo G3 QTof

This presentation aims to highlight some of the developments and enhancements that have been made to the recently launched Xevo G3 QTof.

HR MSE Toxicology Screening using the Waters Xevo G3 QTof

This webinar we will help you to understand more about the latest advancements in sample preparation strategies to enable accurate PFAS determination in challenging food matrices.
PFAS bioaccumulate within in the environment and ultimately manifest as PFAS contamination in the food chain. In recent years, significant contamination has been reported in meat, fish, dairy produce fruit, vegetables as well as processed foods.
Even when sophisticated chromatographic separation and mass detection technologies are employed for their analysis, these varied and complex matrices present many challenges for accurate PFAS determination.
Key learning objectives
<ul>
<li>Attendees will learn the latest advancements in sample preparation techniques for difficult food matrices for the analysis of PFAS</li>
<li>Our sample preparation solutions for PFAS analysis have been verified and validated</li>
<li>Attendees will be equipped with a workflow solutions they can implement right away</li>
</ul>
Who should attend?
This is suitable for anyone who is working in a laboratory environment preparing environmental or food samples for PFAS analysis. However, we welcome anybody who would like to simply learn more about sample preparation of food matrices for PFAS analysis.
<blockquote>
Presenter: Nassima Galloway (Business Development Manager Food &amp; Environmental, Agilent Technologies)
</blockquote>
<blockquote>
Nassima Galloway BSc obtained her degree at Manchester University in Medical Biochemistry BSc. Her professional career began working with environmental laboratories within EMEA offering support on sample preparation solutions and helping laboratories to optimize their workflows and throughout.
</blockquote>
<blockquote>
She now holds the position of Business Development Manager for EMEAI within the chemistries supplies division of Agilent Technologies. Nassima works closely within the food &amp; environmental markets to promote the latest advancements in sample preparation techniques and workflow solutions for LC/MS GC/MS.
</blockquote>
<blockquote>
Presenter: Carola Damen (LC/MS Product Specialist Benelux, Agilent Technologies)
</blockquote>
<blockquote>
Carola Damen obtained her master&#39;s degree in pharmacy at the University of Groningen, after which she completed her pharmacist training at the same university. During her studies she specialized fully in the field of analytical chemistry and in particular liquid chromatography coupled with mass spectrometry.
</blockquote>
<blockquote>
Carola obtained her PhD at Utrecht University, where she carried out this PhD program at the Netherlands Cancer Institute in Amsterdam. The research focused on bioanalysis of anti-cancer drugs using LC/MS. After having fulfilled various laboratory functions, Carola has been working as a product specialist LC/MS at Agilent since 2020.
</blockquote>

Agilent Technologies: Analysis of PFAS in the Food Chain

Analysis of PFAS in the Food Chain

Understand more about the latest advancements in sample preparation strategies to enable accurate PFAS determination in challenging food matrices.

Human shelterin is a six-subunit complex that binds telomeres, protects them from the DNA-damage response, and regulates the maintenance of telomeric DNA.
Although high-resolution structures have been generated for individual structured domains of shelterin subunits, the structure and stoichiometry of the full complex is unknown.
In this webinar, Dr. John Zinder, a Senior Scientist in the Structural Biology group at Odyssey Therapeutics, will discuss the purification of shelterin subcomplexes and reconstitution of the entire complex using full-length, recombinant subunits. By combining mass photometry, negative-stain electron microscopy (EM), crosslinking mass spectrometry (XLMS), AlphaFold modelling, and native mass spectrometry (MS), stoichiometries were obtained, as well as domain-scale architectures of shelterin subcomplexes.
In addition, Dr. Zinder will present data on shelterin complexes which feature extensive conformational heterogeneity, regardless of the presence of DNA substrates. He will also explain how shelterin adopts a multitude of conformations and its unusually high structural variability can be beneficial for its many functions at telomeres.
Key learning objectives
<ul>
<li>Learn how to study conformational heterogeneity using single-particle mass analysis, including mass photometry</li>
<li>Understand how modelling can aid in the characterization of highly flexible assemblies, such as shelterin</li>
<li>Discover how mass photometry, especially when combined with complementary techniques, can be a powerful tool for determining the stoichiometry of a complex as well as its compositional heterogeneity at low concentrations</li>
</ul>
Who should attend?
<ul>
<li>Anyone interested in mass photometry, the architecture of shelterin, protein complexes, protein-DNA interactions, telomere stability, structure determination, molecule prediction, homology modeling, and mass spectrometry.</li>
</ul>
<blockquote>
Presenter: Davide Zinder PhD (Odyssey Therapeutics)
</blockquote>
<blockquote>
Dr. John Zinder is a Senior Scientist in the Structural Biology group at Odyssey Therapeutics and works in the development of novel therapeutics. He has an undergraduate degree in Chemistry from Cornell University, and a masters degree in Biochemistry from the University of Colorado. He was awarded a PhD in Chemical Biology from his research in Chris Lima’s lab at the Sloan Kettering Institute. Zinder subsequently became a Damon Runyon Postdoctoral Fellow in the de Lange and Walz labs at the Rockefeller University.
</blockquote>
<blockquote>
Presenter: Lawrence Howes (SelectScience)
</blockquote>
<blockquote>
Lawrence studied biological sciences at Swansea University, UK, before completing a master’s in epidemiology at the University of Bristol. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience
</blockquote>

SelectScience: Architecture and Stoichiometry of Human Shelterin Using Single-Particle EM and Mass Analysis

Architecture and Stoichiometry of Human Shelterin Using Single-Particle EM and Mass Analysis

Purification of shelterin subcomplexes and reconstitution of the entire complex using full-length, recombinant subunits.

Architecture and Stoichiometry of Human Shelterin

Join our panel of PFAS experts from across the food and environmental testing arena as we discuss challenges, best practices, solutions, and the future of analyzing these “forever” chemicals. Exploring the global implications of PFAS testing and ever updating regulatory requirements, hear our experts share their experiences and insights. Be sure to engage in the discussion and overcome your analytical challenges by posting your questions to our panel.
Key learning objectives
<ul>
<li>Key considerations in uncovering the right workflow- from sample preparation, detection and quantification- to achieve quality data you can trust now, and into the future.
</li>
<li>Best practices and tips in maintaining reliable and repeatable analysis in the routine laboratory workspace.
</li>
<li>Comprehensive analytical solutions to meet your laboratory’s demands, increase capacity and outperform even the most stringent of PFAS limits currently in place, US EPA Health Advisory Limits.
</li>
</ul>
Who should attend?
Lab Managers, Quality Managers, Technical Managers, Chief Scientific Officers, Lead Scientists who wish to improve their confidence, capabilities and capacity in PFAS testing.
<blockquote>
Presenter: Dr. Kari Organtini (Waters Corporation)
</blockquote>
<blockquote>
Dr. Kari Organtini is part of the food and environmental applications team at Waters, developing applications on LC- and GC- MS/MS for a variety of applications ranging from pesticides to persistent organic pollutants. She is currently the scientific lead for developing PFAS applications. She received her PhD from Penn State University in 2015 where she studied the occurrence of dioxins and furans in fire debris and their implications to first responder health.
</blockquote>
<blockquote>
Presenter: Dr. Stuart Adams (Waters Corporation)
</blockquote>
<blockquote>
Dr. Stuart Adams has been developing methods for the determination of contaminants in food and the environment for over 17 years. Before he moved to Waters in 2018 to focus on application development, he previously worked as a senior analytical chemist at Fera. He now works with customers to understand and help address their analytical challenges with Waters technologies, with a particular interest in PFAS.
</blockquote>
<blockquote>
Presenter: Stuart Oehrle (Waters Corporation)
</blockquote>
<blockquote>
Stuart Oehrle is a field chemist with over 30 years of tenure at Waters and an adjunct instructor for 25+ years at Northern Kentucky University. Stuart obtained his chemistry degree from Northern Kentucky University and did his graduate work at the University of Alabama-Huntsville and University of Cincinnati. Stuart’s primary area of focus is the application of LC and LC-MS/MS. He has worked extensively in the analyses of toxins from cyanobacteria, explosives, and chemical warfare agents. He has published or presented over 70 papers as well as been awarded several patents.
</blockquote>
<blockquote>
Presenter: Dr. Nicole Riddell (Wellington)
</blockquote>
<blockquote>
Nicole is the Quality Manager at Wellington Laboratories Inc. She obtained a PhD in Analytical Chemistry from Orebro University (Sweden) under the supervision of Dr. Bert van Bavel and a Master’s degree in Synthetic Organic Chemistry from the University of Guelph (Canada). Her main responsibilities include Quality Management in support of Wellington’s three ISO accreditations, provision of technical support relating to the use of Wellington’s certified reference standards, and general research project co-ordination. Her multi-disciplinary background allows her to execute these tasks with a unique perspective. Although Nicole provides support for all Wellington product lines she has worked heavily with per- and polyfluoroalkyl substances (PFAS), in both a synthetic and analytical capacity, since she started at Wellington nearly 18 years ago.
</blockquote>
<blockquote>
Presenter: Ken Rosnack (Waters Corporation)
</blockquote>
<blockquote>
Ken Rosnack is a Principal Market Development Manager in the Testing &amp; Lab Services Group for Waters Corporation. His primary focus is within regulated and routine LC-MS/MS environmental market where he is responsible for world-wide strategy implementation and performance. He maintains collaborations within regulatory agencies as well as with global industrial partners. Over his career, Ken has been primary author on numerous publications, been an invited speaker multi-nationally, and has presented at many conferences. He has been involved with MS for the past 40 years.
</blockquote>
<blockquote>
Presenter: Blake Forman (SelectScience)
</blockquote>
<blockquote>
Blake studied chemistry at the University of Surrey, UK, and further went on to study a master’s in chemistry at the University of Southampton, UK. Blake has previously worked in the Medicines and Healthcare Products Regulatory Agency (MHRA) lab at LGC and as a technical sales specialist for Waters. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.
</blockquote>

Waters Corporation: The highs and lows PFAS testing: An expert panel discussion

The highs and lows PFAS testing: An expert panel discussion

Exploring the global implications of PFAS testing and ever updating regulatory requirements, hear our experts share their experiences and insights.

Adsorption, distribution, metabolism and excretion (ADME) profiling is used to determine the properties of drug candidates. Modern drug discovery environments depend on the accuracy and precision of results from ADME studies to select new targets with a high potential for clinical success.
However, as technology advances, the size of compound libraries increases. Once a compound shows an affinity for a target, understanding the ADME profile of a drug in vitro can reduce attrition rates in the drug development process.
Acoustic ejection mass spectrometry (AEMS) has a significantly higher throughput than traditional LC-MS approaches due to the rapid sampling and flow injection analysis enabled by a high-sensitivity mass spectrometer.
In the webinar, we discuss a suite of metabolic stability assays developed using an AEMS system.
Attend this webinar to: 
Learn about how the Echo® MS system can be used in drug discovery 
Hear about the advantages of AEMS 
Discover the fundamentals of Echo® MS operation and how it can benefit your research
<blockquote>
Presenter: Dr. Jacob W. McCabe (Senior Scientist Echo® MS Center of Excellence, SCIEX)
</blockquote>

Technology Networks: Discover New Ways To Increase Your Throughput for Metabolic Stability Assays

Discover New Ways To Increase Your Throughput for Metabolic Stability Assays

In the webinar, we discuss a suite of metabolic stability assays developed using an AEMS system.

Increase Your Throughput for Metabolic Stability Assays

Polar pesticides are the talk of the town.
And we are here to encounter them and provide a breakthrough LC/MS/MS solution to address the challenges associated with them. Attend this The Lab Central premiere to find out more.
<blockquote>
Presenter: Dr. Prasanth Joseph (Application Engineer - LC/MS, Agilent Technologies)
</blockquote>

Agilent Technologies: The Encounter: Polar Pesticides and LC/MS

The Encounter: Polar Pesticides and LC/MS

We are here to encounter them and provide a breakthrough LC/MS/MS solution to address the challenges associated with polar Pesticides.

Polar Pesticides and LC/MS

What&#39;s the connection between nirosamine impurities and mass spectrometry?
How do you ensure your lab is ready to handle and analyze any API or formulation sample at any time?
Get answers to these questions in this The Lab Central premiere.
<blockquote>
Presenter: Preeti Bharatiya (Application Engineer LC-MS/MS, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Dr. Soma Dasgupta (Application Engineer GC/MS, Agilent Technologies)
</blockquote>

Agilent Technologies: The Nitrosamines Connection

The Nitrosamines Connection

Connection between nirosamine impurities and mass spectrometry.

Examining powdered samples in the presence of a reactive gas or at elevated temperatures could be a difficult measurement to make.
During this workshop, we will be looking at how to make such diffuse reflection measurements using a high-end Cary instrument and an accessory from Harrick Scientific known as the Praying Mantis. Tips and tricks of using this accessory will also be discussed.
<blockquote>
Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>
<blockquote>
Presenter: Sue Berets, PhD (Applications Scientist, Harrick Scientific Products)
</blockquote>
<blockquote>
Sue, an Applications Chemist for Harrick Scientific Products, has over 30 years of experience in accessories and optics for molecular spectroscopy. Sue received her doctorate in physical chemistry from Tufts University and has carried out basic research in theoretical and applied spectroscopy. Her responsibilities include technical and sales support, including accessing products for specific customer applications. She is involved in new product development, from initial concept to finished product, to meet the every changing applications needs of the market.
</blockquote>

Agilent Technologies: Unique Measurement Capabilities Using the Praying Mantis and a Cary UV-Vis system

Unique Measurement Capabilities Using the Praying Mantis and a Cary UV-Vis system

How to make such diffuse reflection measurements using a high-end Cary instrument and an accessory from Harrick Scientific known as the Praying Mantis.

Using the Praying Mantis and a Cary UV-Vis system

Karl Fischer (KF) titration is the primary technique for moisture analysis in many industries.
Although the technique is well-defined, sample preparation and injection are greatly impacted by human error. Even with robust SOPs, slight differences in sample handling by analysts can deliver troubling inconsistencies in results.
Attend this webinar to hear Doug Clark share tips on how you can reduce the impact of human error and improve the accuracy and repeatability of your Karl Fischer results. Doug is an expert with over 30 years of experience in Karl Fischer titration. Our webinar session will conclude with a live question and answer forum moderated by Kerri-Ann Blake, Product Specialist for Titration at Metrohm. This session will answer your questions and present the latest technologies to improve the accuracy of your KF results. 
Key Learning Objectives
<ul>
<li>Learn sample handling techniques to improve KF titration results
</li>
<li>Receive tips for improving KF titration accuracy with proper reagent selection
</li>
<li>Understand how 3rd party devices can be added to titration systems to improve workflow and remove human error
</li>
</ul>
Who Should Attend
<ul>
<li>Analysts performing KF titration who are interested in identifying how human error might be skewing their results
</li>
<li>Lab managers looking to increase accuracy by learning simple tips they can share with their lab staff
</li>
<li>Anyone interested in learning how auxiliary devices, such as an oven, can be integrated into KF titration systems to increase accuracy and repeatability
</li>
</ul>
<blockquote>
Presenter: Doug Clark (Karl Fischer Expert)
</blockquote>
<blockquote>
Doug Clark has over 30 years of experience dedicated to Karl Fischer titration. His extensive career includes developing KF reagents and applications as well as having lead the Honeywell and Sigma Aldrich HYDRANAL Technical Center in St. Louis.
</blockquote>
<blockquote>
Presenter: Dr. Kerri-Ann Blake (Titration Product Manager, Metrohm USA)
</blockquote>
<blockquote>
Dr. Kerri-Ann Blake is the Titration Product Manager at Metrohm USA in Riverview, Florida. She earned her PhD in Materials Science and Engineering at the University of Florida where she was a researcher at the Particle Engineering Research Center. In her years with Metrohm USA, she has performed proof of concept studies, internal and external trainings and provided applications support. Kerri-Ann plays a key role in product support and product marketing and also has experience developing and implementing ASTM methods.
</blockquote>

Metrohm: Impact of Human Error in Karl Fischer Titration

Impact of Human Error in Karl Fischer Titration

How you can reduce the impact of human error and improve the accuracy and repeatability of your Karl Fischer results.