In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with Imad Haidar Ahmad, Scientific Research Director at Amgen leading separations for Discovery Chemistry and Externalization. Imad's career spans Novartis, Merck, and now Amgen, and his research on computer-assisted method development has landed multiple front covers in Analytical Chemistry.We dig into how his group builds retention models from as few as nine lab experiments to map an entire separation landscape — replacing weeks of trial and error with a resolution map you can query and rebuild from. Imad breaks down where this modeling approach struggles (large molecules, SFC, fully autonomous method development), walks through two-dimensional liquid chromatography in plain terms for anyone who's only worked in 1D, and shares the story behind his group's greenness-score mapping work — including where swapping acetonitrile for methanol worked, and where it didn't.We also talk career advice: what a typical day looks like leading a discovery-stage separations team, how undergrad and grad students can bridge the academia-to-industry gap, and what Imad sees as the field's biggest unsolved problem.<h3>Video Transcription</h3>Chromatographic method development is increasingly moving beyond trial and error toward workflows built on modeling, automation, multidimensional separations, and smarter use of data. In a recent interview, Haidar Ahmad discussed how these approaches are changing pharmaceutical analysis, why understanding the fundamentals of chromatography remains essential, and where some of the biggest opportunities in separation science still lie.Ahmad’s route into analytical chemistry was not entirely planned. As an undergraduate, he initially found inorganic and organic chemistry particularly attractive, but a final-year project focused on the analysis of additives in bread introduced him to the possibilities of analytical chemistry. During his PhD, his research moved into polymer analysis and structure–property relationships. His interest in chromatography deepened when he encountered two-dimensional liquid chromatography (2D-LC), particularly its ability to reveal forms of polymer heterogeneity that could not be adequately characterized using a single separation technique. This eventually led to postdoctoral work with Peter Carr, where Ahmad focused on chromatographic separations and the fundamentals of chromatography.His subsequent career in the pharmaceutical industry exposed him to different stages of drug development, from late-stage method development and validation to drug substance characterization, purification, and finally discovery-stage separations. That breadth of experience has shaped his view of chromatography as both a scientific discipline and a practical problem-solving tool. In late-stage development, the objective may be a highly robust method capable of successful validation and transfer between laboratories. In discovery, by contrast, speed becomes critical because teams may be working with thousands of molecules while trying to identify the best candidates to advance.&nbsp;<h4>Discovery-stage chromatography: speed, prioritization, and throughput</h4>For a separations team supporting drug discovery, the working day is shaped by a constant flow of requests from medicinal chemistry and other partner groups. The challenge is not simply to perform the analyses, but to decide which requests are most urgent, remove bottlenecks, communicate with project teams, and continuously improve existing workflows.According to Ahmad, one of the most important questions is whether a task can be done faster or in a more high-throughput way. Discovery laboratories operate under strong time pressure, and efficiency therefore becomes part of the scientific strategy rather than merely an operational concern. As he describes it, the work combines science, prioritization, leadership, and communication, all with the aim of moving the most important projects forward as quickly as possible.<h4>In silico modeling: letting the computer perform the experiments</h4>One of the central themes of Ahmad’s work is in silico chromatographic modeling. In practical terms, the approach begins with a carefully designed set of laboratory experiments—often around nine runs. The resulting data are fed into software that builds retention models describing how analyte retention changes with variables such as mobile-phase composition and temperature.The software can then generate a resolution map representing a much larger separation space than could realistically be tested experimentally. Instead of running dozens or hundreds of conditions by trial and error, the analyst can identify regions where critical peak pairs are predicted to achieve the best separation.For Ahmad, the value of these maps goes beyond simply finding the condition with the highest resolution. Each point on the map represents a chromatographic condition, while the visual output shows where poor and strong separations are expected. A broad region of good resolution is particularly useful because it points toward a more robust operating space rather than a narrowly defined optimum that might be sensitive to small variations in experimental conditions.He therefore prefers to think of a chromatographic model as a map or database, rather than simply an optimization tool. If an impurity later disappears from the process, the corresponding peak can be removed from the model and new conditions selected without repeating the complete experimental study. Conversely, if a new degradation product appears during stability studies, the peak can be added and the existing model used to search for updated separation conditions.<h4>Why trial and error still has a place</h4>Modeling does not mean that conventional trial-and-error method development has disappeared. Ahmad points out that it remains useful in early discovery, particularly for fit-for-purpose methods where speed is more important than achieving a fully optimized separation.The situation changes later in development. A method intended for validation, transfer, or long-term routine use carries much greater risk. Trial and error may identify a condition that appears satisfactory without revealing whether it represents the most robust region of the separation space. If that method later fails during transfer or routine operation, the consequences can be costly.For late-stage methods, the additional investment required to build a model can therefore reduce risk over the much longer lifetime of the analytical procedure.<h4>Models are only as good as the experiments behind them</h4>Chromatographic modeling is not automatic proof against poor method development. Ahmad compares the technology to a GPS: it is highly useful, but only if the analyst understands where they are trying to go.Careful experimental design remains essential. Incorrect dwell-volume or column-volume information, as well as insufficient column re-equilibration, can produce misleading predictions. Importantly, these problems may not be obvious when the experimental runs used to construct the model are compared with their calculated values. They may emerge only when the software begins predicting new conditions within the modeled design space.Modeling is also easier for some separations than others. Ahmad describes reversed-phase LC of small molecules as comparatively straightforward, while large biomolecules are more challenging because their retention can be highly sensitive to small changes in mobile-phase composition and temperature.Other separation modes present additional difficulties. Ahmad notes that his teams have found supercritical fluid chromatography (SFC) particularly challenging to model successfully. Fully autonomous method development remains another unresolved goal: current tools can automate parts of optimization, but analyst input is still required.<h4>Why two dimensions can reveal what one dimension cannot</h4>Ahmad’s early interest in 2D-LC came from polymer characterization. He explains the principle simply: one-dimensional chromatography may answer one question about a sample, while two complementary dimensions can answer two.Two copolymers, for example, may have similar molecular size and therefore coelute in size-exclusion chromatography. A second separation mechanism based on interaction with a stationary phase may distinguish them according to chemical composition. Combining both dimensions provides a more complete description of complex distributions involving molecular weight, composition, sequence, and other forms of heterogeneity.Historically, the complexity of both instrumentation and software limited wider adoption of 2D-LC. Ahmad recalls systems requiring several computers and multiple software packages. That situation has improved substantially, and 2D-LC is now easier to implement across research and pharmaceutical development.Nevertheless, modeling multidimensional separations still presents challenges. While models can be built for individual fractions or dimensions, current software does not easily identify a common optimum across multiple models simultaneously. Much of this comparison still requires manual work.<h4>Bringing sustainability into method development</h4>Another area where Ahmad sees room for change is green analytical chemistry.Traditional pharmaceutical method development has understandably prioritized resolution, sensitivity, robustness, and turnaround time. Sustainability has often been treated as a secondary consideration, particularly at the analytical scale, where solvent use appears small compared with preparative chromatography or manufacturing.That mindset is beginning to change. Ahmad argues that greenness metrics should increasingly be incorporated directly into method-development software. If analysts could immediately see how changes in flow rate, gradient conditions, solvent choice, or other parameters affect the environmental profile of a method, sustainability could become part of routine optimization rather than an assessment performed after the method has already been developed.The objective, however, is not to pursue the greenest possible method regardless of analytical performance. Ahmad emphasizes the need to develop methods that are as green as possible while remaining fit for purpose. Modeling can potentially help balance these competing requirements by identifying conditions that preserve separation performance while allowing greener solvents or alternative chromatographic conditions to be introduced.<h4>Advice for young chromatographers: do not treat HPLC as a black box</h4>For students learning chromatography, Ahmad’s central recommendation is straightforward: do not treat the HPLC system as a black box.Rather than collecting chromatograms by making random changes, he encourages analysts to build a mental model of the separation. When temperature, gradient, or another parameter is changed, they should ask why retention or selectivity changed and use each experiment to improve their understanding of the system.He also recommends making use of educational resources on chromatographic fundamentals and method development and, importantly, not being afraid to experiment with instrument conditions. Modern systems are robust, but the analyst still needs to understand the underlying separation science in order to use them effectively.The same proactive attitude applies to career development. Ahmad strongly encourages students to seek industry internships and make connections beyond their own institutions. Conferences and poster sessions can provide natural opportunities to meet scientists from industry, discuss research, and ask about internship opportunities. Based on his own experience working with undergraduate and graduate interns, these collaborations can benefit both students and research teams and may strongly influence later academic or career decisions.<h4>What still needs to be solved?</h4>Asked about the biggest unresolved challenges in separation science, Ahmad does not begin with technology. Instead, he points to a shortage of well-trained analytical chemists with a strong understanding of chromatographic fundamentals.Alongside education, he identifies several technical opportunities. One is truly autonomous method development, where an instrument and software could optimize a separation without continuous analyst input. Another is the ability to predict chromatographic selectivity directly from molecular structure.Such capability could be especially valuable in high-throughput discovery environments. If a laboratory could predict which stationary phase and conditions were most likely to separate a particular set of compounds or isomers before entering the laboratory, method development could become considerably faster and more efficient.Taken together, Ahmad’s perspective points toward a future in which chromatography becomes increasingly model-driven, automated, multidimensional, and sustainability-aware. But the technology does not eliminate the need for chromatographic knowledge. On the contrary, as analytical tools become more powerful, understanding the fundamentals may become even more important—the software can explore the separation space, but the scientist still needs to know what question to ask and how to interpret the answer.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 In Silico Modeling, 2D-LC & Green Chromatography: Imad Ahmad on the Future of Method Development

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

Researchers from CATRIN at Palacký University, together with colleagues from VSB – Technical University of Ostrava and Charles University, have developed a new graphene ink for producing inexpensive and sensitive printed sensors. Using single-atom engineering, they incorporated copper atoms into the material, enabling highly sensitive detection of dopamine – a neurotransmitter responsible for transmitting signals between nerve cells. The approach, <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">presented in the journal Advanced Science</a>, has strong potential for use in medical diagnostics.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp 117w," sizes="100vw" width="1920" height="2560">Single-atom engineering makes it possible to control the chemical reactivity of materials at the level of individual atoms. Its broader application in medical diagnostics has so far been limited by a lack of atomically engineered materials and printable inks based on them. Researchers have now developed a new class of single-atom-doped graphene materials as well as an ink for producing sensitive printed sensors. Copper proved particularly effective for dopamine detection, which is important for biomedical research and the development of advanced diagnostic technologies for diseases including Alzheimer’s disease.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp 117w," sizes="100vw" width="1920" height="2560">“The NGA-Cu ink we developed enables the production of fully inkjet-printed, sustainable paper-based electrodes at very low cost. Each sensor requires only 1.3 micrograms of active ink, costs approximately one Czech koruna and provided a stable response for at least three months. The ink is also fully compatible with commonly available materials inkjet printers. This creates significant potential for producing affordable, digitally designed sensors whose function can be tailored atom by atom,” said&nbsp;the corresponding author of the <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">paper</a> Michal Otyepka.The basis for this new generation of single-atom inks was nitrogen-doped graphene acid (NGA), which CATRIN researchers developed several years ago. They have now progressively enriched it with different individual metal atoms, including copper, iron and manganese.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp 117w," sizes="100vw" width="1920" height="2560">“We found that the electrochemical response depends fundamentally on the type of atom used. While copper atoms significantly enhance the dopamine signal, other metals suppress it. What matters, therefore, is not only the presence of isolated metal atoms but also the specific metal involved. This is a very important finding for the further development of biosensors,” explained first author Martin-Alex Nalepa.The published results introduce a new class of sensing materials with considerable potential for medical diagnostics. The researchers present a broadly applicable platform for developing graphene inks containing individual metal atoms and open the way towards scalable, reproducible and material-efficient production of advanced electrochemical sensors.&nbsp;“We are only at the beginning and are fascinated by the new possibilities opening up before us. We can see that by changing the type of atom, we can control the sensitivity and selectivity of the sensor. In addition to dopamine, we can also determine immunoglobulins very precisely – antibodies whose levels and types may serve as important indicators of autoimmune or infectious diseases,” emphasised Radek Zbořil, a co-author of the paper.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp 117w," sizes="100vw" width="1920" height="2560">Electrochemical biosensors can find applications not only in medicine but also in food quality control, drinking-water monitoring and environmental pollution monitoring. Their key components are functionalised electrodes, and graphene-based materials have proved particularly suitable for their production. A key factor in the success of the Olomouc researchers was the targeted preparation of graphene ink with the required properties.The research was supported by the TECHSCALE project under the Johannes Amos Comenius Programme.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics: Project TECHSCALE (No. CZ.02.01.01/00/22_008/0004587) is financed by the MEYS OP JAC Excellent research programme, supported by ERDF/ESF." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg 245w," sizes="100vw" width="620" height="87"><blockquote><h4>Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">Single-Atom-Enhanced Fully Inkjet-Printed Electrochemical Sensor for Dopamine Detection</a></h4>Martin-Alex Nalepa, David Panáček, Vítězslav Hrubý, Matěj Jendřišák, Rostislav Langer, Michal Langer, Petr Jakubec, Ivan Dědek, Vojtěch Kupka, Michal Mazur, Radek Zbořil, Michal OtyepkaAdv. Sci., 2026, e76796<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/advs.76796">https://doi.org/10.1002/advs.76796</a><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Single-atom (SA) engineering offers atomic-level control over interfacial reactivity, yet the lack of printable SA-based inks hinders its practical translation into electrochemical sensing. Here, a fully water-based inkjet-printable ink is introduced based on nitrogen-doped graphene acid (NGA) hosting atomically dispersed Cu centers (NGA-Cu-ink). The ink enables digitally controlled, spatially defined deposition and fabrication of low-cost ($0.04 per sensor), fully inkjet-printed sustainable electrodes on paper. A comparison of NGA functionalized with different SA dopants (Cu, Mn, Fe, Ce) identifies a strong dopant-dependent electrochemical response, with Cu uniquely enhancing the analyte signal while other dopants suppress it, demonstrating that SA identity is a decisive design parameter in printed sensing interfaces profiling sensitivity and selectivity. The functional role of the NGA support is to provide dense anchoring sites (nitrogen and carboxylate groups) that stabilize atomically dispersed metal centers and create adsorption- and electron-transfer–active microenvironments. The NGA-Cu-ink yields enhanced dopamine oxidation, enabling quantitative detection on fully printed devices (limit of detection 9.7 µM; linear range 50–400 µM) and printed-on-electrode platforms (10.6 µM), while maintaining &lt;10% signal variation over 11 weeks. The approach establishes a general route to single-atom graphene-based inks for scalable, reproducible, and low-material-consumption manufacturing of advanced electrochemical sensors.

Atom by atom. Scientists reveal a new direction in medical diagnostics

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The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Tosoh Bioscience

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Agilent Technologies

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Waters Corporation

UV-Vis measurements you want to make do not always fall into the realm of a liquid in a standard cuvette.
When this is the case, the ability to add an accessory to the sample compartment or in the case of the Cary 3500, swap out a sampling Module, is key to increasing your measurement capabilities. This presentation will explore various options for expanding measurement capabilities, especially the Cary 3500 with the recently introduced Flexible Module.
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Presenter: Mark Fisher, PhD (Application Engineer, Molecular Spectroscopy, Agilent Technologies, Inc.)
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<blockquote>
Mark, an Application Scientist for Agilent Technologies, has over 35 years of experience in molecular spectroscopy. He has held roles in Agilent&#39;s UV-Vis-NIR, fluorescence, and atomic absorption product portfolios. In addition to identifying industry trends and new business needs, Mark&#39;s responsibilities include application development, pre and post-sales support, as well as acting as a customer advocate for new product and technology opportunities. Mark received his doctorate in analytical chemistry from the University of Nebraska-Lincoln.
</blockquote>

agilent Technologies: Expanding Your UV-Vis Measurement Capabilities through the use of Accessories

Expanding Your UV-Vis Measurement Capabilities through the use of Accessories

Explore various options for expanding measurement capabilities, especially the Cary 3500 with the recently introduced Flexible Module.

Expanding Your UV-Vis Measurement Capabilities

Demonstration of an automated Protein A purification and LC-MS mAb subunit analysis of bioreactor samples to screen biosimilar monoclonal antibody (mAb) candidates in comparison to the innovator to support process development.
The method was demonstrated to have sufficient sensitivity and precision to quantitively assess N-linked oligosaccharides at levels known to impact pharmacokinetics and effector function activity, in addition to other post-translational modifications (PTMs) such as C-terminal lysine variants and other subunit-specific (LC &amp; Fd’) modifications
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Presenter: Jared Young (Similis Bio)
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Waters Corporation: "Hear a customer perspective on deploying Waters solutions to enhance biosimilar development: Streamlined mAb Subunit LC-MS Workflow for Multiple Attribute Monitoring of Biosimilar mAb Candidates During Bioprocessing and Development"

Streamlined mAb Subunit LC-MS Workflow for Multiple Attribute Monitoring of Biosimilar mAb Candidates During Bioprocessing and Development

Hear a customer perspective on deploying Waters solutions to enhance biosimilar development.

Streamlined mAb Subunit LC-MS Workflow for mAb

In modern pharmaceutical analysis, the utilization of Pharmacopeia regulated U/HPLC (ultra/high-performance liquid chromatography) methods has become a fundamental aspect of laboratory operations.
This webinar aims to explore the diverse strategies and techniques that can be employed to fully leverage the potential of U/HPLC method adaptation, thereby enhancing the effectiveness and efficiency of laboratory processes. The presentation delves into the principles of U/HPLC and highlights the significance of adhering to Pharmacopeia regulations for ensuring reliable and accurate analytical results. Furthermore, various approaches for method adaptation, such as modification of mobile phase composition, adjustment of column parameters, and optimization of detection conditions will be discussed, including the benefits and challenges associated with each adaptation strategy and insights into the factors influencing method transfer and development.
Additionally, the potential applications of U/HPLC method adaptation in pharmaceutical quality control, impurity profiling, and stability testing will be explored. By understanding the nuances and intricacies of U/HPLC method adaptation, laboratories can unlock new avenues for improving productivity, reducing costs, and enhancing the overall quality of pharmaceutical analysis.
By attending this presentation you will:
<ul>
<li>Learn about the principles and importance of U/HPLC method adaptation in pharmaceutical analysis and how improved methods can help to boost laboratory effectiveness and efficiency
</li>
<li>Gain insights into the significance of adhering to Pharmacopeia regulations for ensuring reliable and accurate analytical results
</li>
<li>Understand various strategies and techniques for adapting U/HPLC methods, such as modifying mobile phase composition, and adjusting column parameters
</li>
<li>Leverage the full potential of U/HPLC method adaptation leading to improved productivity and cost reduction in the laboratory
</li>
</ul>
Who should attend
<ul>
<li>Lab managers and technicians working with HPLC/UHPLC methods (R&amp;D, QC and method transfer).</li>
</ul>
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Presenter: 
</blockquote>
<blockquote>
Presenter: Petra Lewits (Global Product Manager for Analytical Chromatography, HPLC columns, Merck KGaA, Darmstadt, Germany)
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Petra Lewits has worked with chromatography for 40 years. In 1986 she joined the pharmaceutical R&amp;D and QC department (Merck KGaA, Darmstadt, Germany) where she developed HPLC methods for R&amp;D and QC of pharmaceutical APIs and formulations as well as for diet convenience foods. Since 2002 she has been a Global Product Manager for Analytical Chromatography (HPLC). In 2014 she began focusing on thin-layer chromatography (TLC). Since April 2018 Petra has had global responsibility for analytical HPLC columns.
</blockquote>
<blockquote>
Presenter: Egidijus Machtejevas (Senior Technical Advisor, Analytical Chromatography Workflows, Merck KGaA, Darmstadt, Germany)
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<blockquote>
Egidijus Machtejevas joined the R&amp;D Department at Merck KGaA, Darmstadt, Germany in 2008. A year later he became marketing manager for North America, and in 2010–2018 Product Manager for chromatography. Currently he is a Senior Technical Advisor in the Analytical Chromatography Workflows group. Egidijus has twenty-five scientific papers and thirteen book chapters to his name, and his major focus areas include chromatography, multidimensional liquid chromatography, proteomics and mass spectrometry.
</blockquote>

Pixabay/Ri Butov: Leverage the full potential of Pharmacopeia regulated U/HPLC method adaptation to increase lab effectiveness

Leverage the full potential of Pharmacopeia regulated U/HPLC method adaptation to increase lab effectiveness

Explore the diverse strategies and techniques that can be employed to fully leverage the potential of U/HPLC method adaptation.

Leverage the full potential of Pharmacopeia

Key Learning Points:
<ul>
<li>Receive an overview of theory of HPLC mobile phase/solid phase and their interactions 
</li>
<li>Learn the differences between mobile phase options, and what to consider when picking a mobile phase 
</li>
<li>Review the of panel results and how mobile phases affected ionization and separation
</li>
</ul>
Who Should Attend:
<ul>
<li>Any HPLC and LC-MS/MS scientists in clinical and toxicology looking to learn more about the theory of HPLC mobile phase</li>
</ul>
<blockquote>
Presenter: Mackenzie Freige (Application Scientist - Clinical, Phenomenex)
</blockquote>
<blockquote>
Mackenzie is an Application Scientist at PhenoLogix, and is based in Torrance, CA. She has undergraduate degrees in Chemistry and in Life Sciences. She joined Phenomenex in 2021, and brings extensive experience in liquid chromatography-mass spectrometry. In her free time she practices yoga and enjoys reading. 
</blockquote>

Phenomenex: HPLC Method Development – Choosing a Mobile Phase for Your Assay

HPLC Method Development – Choosing a Mobile Phase for Your Assay

Receive an overview of theory of HPLC mobile phase/solid phase and their interactions.

HPLC Method Development – Choosing a Mobile Phase

This popular online event will once again bring together leading scientists in the field as they highlight the latest advancements in translational proteomics and metabolomics. Presentations will cover areas ranging from uncovering the molecular mechanisms of disease pathology, to the latest technological developments in diagnostics and treatment.
This online symposium will explore:
<ul>
<li>Cutting-edge proteomics for brain aging and dementia biomarkers</li>
<li>Innovations in single-cell proteomics technology</li>
<li>Next-gen protein sequencing and PTM detection</li>
<li>Healthcare transformation through data analytics and remote monitoring</li>
</ul>
<h3>Agenda</h3>
<h5>16:00 – 16:40 (BST) Keynote: Transforming Healthcare Using Deep Data and Remote Monitoring</h5>
<ul>
<li>Keynote Speaker: Prof. Michael Snyder</li>
</ul>
<h5>16:40 – 16:50 Networking Break</h5>
<h5>16:50 – 17:35 Challenges In Human Metabolomics Studies and Standardizing Quantitative Metabolomics Globally</h5>
<ul>
<li>Speakers: Prof. Jerzy Adamski &amp; Markus Langsdorf, PhD</li>
</ul>
<h5>17:35 – 17:45 Networking Break</h5>
<h5>17:45 – 18:35 Proteomics as a Tool to Identify Drivers and Biomarkers of Brain Aging and Dementia</h5>
<ul>
<li>Speaker: Keenan Walker, PhD</li>
</ul>
<h5>18:35 – 18:45 Networking Break</h5>
<h5>18:45 – 19:05 Advancing Protein Analysis with Next-Generation Protein Sequencing™</h5>
<ul>
<li>Speaker: Ben Moree, PhD</li>
</ul>
<h5>19:05 – 19:15 Networking Break</h5>
<h5>19:15 – 20:05 Improved Sample Preparation, Separations and Data Acquisition for Single-Cell Proteomics</h5>
<ul>
<li>Speaker: Ryan Kelly, PhD</li>
</ul>
<h5>20:05 – 20:15 Networking Break</h5>
<h5>20:15 – 20:55 Identification and Analysis of Non-Invasive Biomarkers in Exhaled Breath</h5>
<ul>
<li>Speaker: Tina Chou, PhD</li>
</ul>

Technology Networks: Advances in Proteomics & Metabolomics 2023

Advances in Proteomics & Metabolomics 2023 - Day1

Areas ranging from uncovering the molecular mechanisms of disease pathology, to the latest technological developments in diagnostics and treatment.

This popular online event will once again bring together leading scientists in the field as they highlight the latest advancements in translational proteomics and metabolomics. Presentations will cover areas ranging from uncovering the molecular mechanisms of disease pathology, to the latest technological developments in diagnostics and treatment.
This online symposium will explore:
<ul>
<li>Cutting-edge proteomics for brain aging and dementia biomarkers</li>
<li>Innovations in single-cell proteomics technology</li>
<li>Next-gen protein sequencing and PTM detection</li>
<li>Healthcare transformation through data analytics and remote monitoring</li>
</ul>
<h3>Agenda</h3>
<h5>16:00 – 16:40 Deciphering Microbial Omics Signatures in Multidimensional Mass Spectrometry</h5>
<ul>
<li>Speaker: Aivett Bilbao, PhD</li>
</ul>
<h5>16:40 – 16:50 Networking Break</h5>
<h5>16:50 – 17:10 Precision Proteomics Accelerates the Early Detection of Dementia</h5>
<ul>
<li>Speaker: Mariana Fontes, PhD</li>
</ul>
<h5>17:10 – 17:20 Networking Break</h5>
<h5>17:20 – 18:10 Towards the Making of Molecular Movies Directed by the Circulating Proteome</h5>
<ul>
<li>Speaker: Prof. Jochen Schwenk</li>
</ul>
<h5>18:10 – 18:20 Networking Break</h5>
<h5>18:20 – 18:40 Lipidomic Variabilities of Human Blood Plasma</h5>
<ul>
<li>Speaker: Prof. Markus Wenk</li>
</ul>
<h5>18:40 – 18:50 Networking Break</h5>
<h5>18:50 – 19:30 The SEISMIC Facility for Spatially Resolved Single and Sub-Cellular Omics</h5>
<ul>
<li>Speaker: Prof. Melanie Bailey</li>
</ul>

Advances in Proteomics & Metabolomics 2023 - Day 2

This presentation will provide an overview of analytical quality by design (AQbD) principles and the evolution of QbD in the pharmaceutical development environment.
A brief introduction to the new USP chapter &lt;1220&gt; Analytical Procedures Life Cycle will also be given, including a short case study on stage 1 — procedure design. Additionally, key aspects of the new ICH Q14/Q2(R2) draft guidelines will be discussed along with their implications.
<blockquote>
Presenter: Amanda Guiraldelli, PhD (Scientific Affairs Manager, U.S. Pharmacopeia)
</blockquote>
<blockquote>
Amanda Guiraldelli has been with USP since 2012 and holds the position of scientific affairs manager and principal scientist in the compendial science group-general chapters. She also serves as scientific liaison for the USP Measurement and Data Quality Expert Committee, where she works to develop and revise USP standards related to analytical procedure lifecycle/validation.
</blockquote>
<blockquote>
Previously, Amanda worked as senior scientist at the USP reference standard laboratory for 8 years with characterization of compendial standards. She is visiting professor at the Institute of Chemistry at the University of Campinas (UNICAMP) in Brazil and is a frequent speaker and instructor on topics related to analytical procedure life cycle, analytical quality by design (AQbD) and chromatography. Amanda is a specialist in mass spectrometry, chromatography, omics, and chemometrics and has more than 15 years of experience in pharmaceutical R&amp;D areas.
</blockquote>
<blockquote>
Presenter: Manu Grover (M.S. (Pharm), Business Development Manager, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Manu has more than 21 years of pharmaceutical industry and chromatography experience. He has worked in the fields of manufacturing and analytical research to support IND, NDA, and ANDA filings in pharmaceutical companies like Dr. Reddy&#39;s, Panacea Biotec, and Ozone Pharmaceuticals. In the course of his professional journey, Manu has developed and validated many analytical methods on HPLC, GC, particle sizing, DSC, pXRD, LC/MS, and more. He has successfully filed multiple analytical dossiers in the US and EU markets, filed analytical methods to USP, and handled multiple US FDA audits.
</blockquote>

Agilent Technologies: Introduction to USP 1220 Analytical Procedures Life Cycle and Implications of ICH Q14 and Q2(R2) Guidelines

Introduction to USP 1220 Analytical Procedures Life Cycle and Implications of ICH Q14 and Q2(R2) Guidelines

Analytical quality by design (AQbD) principles and the evolution of QbD in the pharmaceutical development environment.

Introduction to USP 1220 Analytical Procedures Life Cycle

In this on-demand webinar, Wai-Chi Man, Product Marketing Manager at Thermo Fisher Scientific, discussed the basics of why Ion Chromatography coupled with Mass Spectrometry (IC-MS) can be an added benefit to any High-Performance Liquid Chromatography with Mass Spectrometry (LC-MS), and complimentary to any Hydrophilic Interaction Liquid Chromatography (HILIC) analysis.
You can also expect to:
<ul>
<li>Enhance your understanding of the instrument requirements for IC and MS: which one is best for your application of interest.
</li>
<li>Learn how to set up the connectivity or simply switch from IC-MS to LC-MS.
</li>
<li>Explore the switch over from applications such as Polar Pesticides from Anionic to Cationic with one instrument set-up, and how it’s applicable to other applications such as Metabolomics.
</li>
</ul>
<blockquote>
Presenter: Wai-chi Man (Product Marketing Manager, Thermo Fisher Scientific)
</blockquote>
<blockquote>
Wai-Chi Man, MRSC has been with Thermo Fisher Scientific since 2011. She started on the application sales support team but is now a product marketing manager. She has specialized in ion chromatography (IC) for more than 33 years, with over 36 years of experience in analytical chemistry in a wide variety of techniques. Before Thermo Fisher Scientific, she spent seven years at Wellcome Research supporting structural elucidation for medicinal chemists using a wide range of analytical instrumentation. Wai-Chi spent a total of 20 years in the pharma industry as part of GlaxoSmith Kline, from research to transferring methods to manufacturing. She worked on and was responsible for many drugs from anti-viral to respiratory in the market today.
</blockquote>
<blockquote>
Wai-Chi’s work at Thermo Fisher Scientific has supported a wide range of customers from various industries, providing IC support in method development, troubleshooting, sample preparation, trainings, seminars, events, and workshops. This experience has helped her build greater knowledge, skill and understanding within areas such as IC-MS, IC-ICP-MS, HPAE-PAD analysis, metabolomics, and polar pesticides.
</blockquote>

Thermo Scientific: Back to Basics – IC-MS 

Back to Basics – IC-MS 

The basics of why Ion Chromatography coupled with Mass Spectrometry (IC-MS) can be an added benefit to any High-Performance Liquid Chromatography.

There are GxP regulations specifically relating to the controls required for the use of electronic records, electronic signatures, and computerized systems. These regulations require a combination of procedural, technical, and behavioral controls to achieve full and ongoing compliance.
It is important for Quality Units and regulators to understand the capabilities available for processing data and, the need for varied levels of flexibility within the Empower Software Solutions based on the chromatography and types of molecules being analyzed. There can be a perception that analysts may use Processing Method parameters or manual integration to introduce human bias, or even “polish results” in a way that would cause otherwise out-of-specification (OOS) samples to pass laboratory test requirements such as assays and impurity methods. To fully ascertain what is happening in the laboratory, both the Quality Unit and regulators should be fully aware of how an organization utilizes the Review window to optimize Processing Method parameters.
In this webinar we will examine the current system policies and privileges in Empower Software that affect what analysts can and cannot do when processing data, particularly in Review.
Please join us for a live discussion. As always, don’t forget to bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.
<blockquote>
Presenter: Neil Lander (Principal Product Manager - Informatics Group, Waters Corporation)
</blockquote>
<blockquote>
Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager in Waters in the Informatics group. Neil provides internal support for Empower CDS software and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters Corporation, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.
</blockquote>
<blockquote>
Presenter: Nisar Ahmed (Empower Applications Consultant, Waters Corporation)
</blockquote>
<blockquote>
Nisar joined Waters Corporation in 1998 and has held a variety of technical positions including Technical Support Specialist, Customer Education Manager and currently Empower Applications Consultant. Nisar works with Waters as an Informatics Consultant. His current role is to act as a resource across Europe providing support, consultancy and training to laboratories implementing and/or using Empower Software as their CDS in a compliant and regulated environment. Nisar joined Waters after a career in the pharmaceutical industry as an analytical chemist. He also holds a Master of Science degree from the University of London.
</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Configuring Empower Software to Maximize Your Compliance

Empower Tips: Ask Me Anything About Configuring Empower Software to Maximize Your Compliance

Current system policies and privileges in Empower Software that affect what analysts can and cannot do when processing data, particularly in Review.

Ask Me Anything About Configuring Empower Software

Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.
Join Marissa Heino, Product Specialist MCC, and Sebastian Thürmann, Product Manager MCC, as they dive into the process of transitioning from an established batch capture step to a multi-column chromatography (MCC) method with ProComposer MethodWizard software and MCC specific pre-packed columns to be run on Octave BIO. They will discuss scale-up using the ProComposer software and explore its integration with the newly launched GMP-ready skid, the Octave PRO, with GMP ready pre-packed columns. Finally, they will provide insight into the potential this technology has to enhancing workflow.
<blockquote>
Presenter: Christian Rohrer (Director of Sales &amp; Support – EMEA)
</blockquote>
<blockquote>
Presenter: Marissa Heino (Product Specialist MCC)
</blockquote>
<blockquote>
Presenter: Sebastian Thürmann (Product Manager MCC)
</blockquote>

Tosoh Bioscience: Multi-Column Chromatography Scale-Up with the Octave Platform

Multi-Column Chromatography Scale-Up with the Octave Platform

Process of transitioning from an established batch capture step to a multi-column chromatography (MCC) method.