In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with Imad Haidar Ahmad, Scientific Research Director at Amgen leading separations for Discovery Chemistry and Externalization. Imad's career spans Novartis, Merck, and now Amgen, and his research on computer-assisted method development has landed multiple front covers in Analytical Chemistry.We dig into how his group builds retention models from as few as nine lab experiments to map an entire separation landscape — replacing weeks of trial and error with a resolution map you can query and rebuild from. Imad breaks down where this modeling approach struggles (large molecules, SFC, fully autonomous method development), walks through two-dimensional liquid chromatography in plain terms for anyone who's only worked in 1D, and shares the story behind his group's greenness-score mapping work — including where swapping acetonitrile for methanol worked, and where it didn't.We also talk career advice: what a typical day looks like leading a discovery-stage separations team, how undergrad and grad students can bridge the academia-to-industry gap, and what Imad sees as the field's biggest unsolved problem.<h3>Video Transcription</h3>Chromatographic method development is increasingly moving beyond trial and error toward workflows built on modeling, automation, multidimensional separations, and smarter use of data. In a recent interview, Haidar Ahmad discussed how these approaches are changing pharmaceutical analysis, why understanding the fundamentals of chromatography remains essential, and where some of the biggest opportunities in separation science still lie.Ahmad’s route into analytical chemistry was not entirely planned. As an undergraduate, he initially found inorganic and organic chemistry particularly attractive, but a final-year project focused on the analysis of additives in bread introduced him to the possibilities of analytical chemistry. During his PhD, his research moved into polymer analysis and structure–property relationships. His interest in chromatography deepened when he encountered two-dimensional liquid chromatography (2D-LC), particularly its ability to reveal forms of polymer heterogeneity that could not be adequately characterized using a single separation technique. This eventually led to postdoctoral work with Peter Carr, where Ahmad focused on chromatographic separations and the fundamentals of chromatography.His subsequent career in the pharmaceutical industry exposed him to different stages of drug development, from late-stage method development and validation to drug substance characterization, purification, and finally discovery-stage separations. That breadth of experience has shaped his view of chromatography as both a scientific discipline and a practical problem-solving tool. In late-stage development, the objective may be a highly robust method capable of successful validation and transfer between laboratories. In discovery, by contrast, speed becomes critical because teams may be working with thousands of molecules while trying to identify the best candidates to advance.&nbsp;<h4>Discovery-stage chromatography: speed, prioritization, and throughput</h4>For a separations team supporting drug discovery, the working day is shaped by a constant flow of requests from medicinal chemistry and other partner groups. The challenge is not simply to perform the analyses, but to decide which requests are most urgent, remove bottlenecks, communicate with project teams, and continuously improve existing workflows.According to Ahmad, one of the most important questions is whether a task can be done faster or in a more high-throughput way. Discovery laboratories operate under strong time pressure, and efficiency therefore becomes part of the scientific strategy rather than merely an operational concern. As he describes it, the work combines science, prioritization, leadership, and communication, all with the aim of moving the most important projects forward as quickly as possible.<h4>In silico modeling: letting the computer perform the experiments</h4>One of the central themes of Ahmad’s work is in silico chromatographic modeling. In practical terms, the approach begins with a carefully designed set of laboratory experiments—often around nine runs. The resulting data are fed into software that builds retention models describing how analyte retention changes with variables such as mobile-phase composition and temperature.The software can then generate a resolution map representing a much larger separation space than could realistically be tested experimentally. Instead of running dozens or hundreds of conditions by trial and error, the analyst can identify regions where critical peak pairs are predicted to achieve the best separation.For Ahmad, the value of these maps goes beyond simply finding the condition with the highest resolution. Each point on the map represents a chromatographic condition, while the visual output shows where poor and strong separations are expected. A broad region of good resolution is particularly useful because it points toward a more robust operating space rather than a narrowly defined optimum that might be sensitive to small variations in experimental conditions.He therefore prefers to think of a chromatographic model as a map or database, rather than simply an optimization tool. If an impurity later disappears from the process, the corresponding peak can be removed from the model and new conditions selected without repeating the complete experimental study. Conversely, if a new degradation product appears during stability studies, the peak can be added and the existing model used to search for updated separation conditions.<h4>Why trial and error still has a place</h4>Modeling does not mean that conventional trial-and-error method development has disappeared. Ahmad points out that it remains useful in early discovery, particularly for fit-for-purpose methods where speed is more important than achieving a fully optimized separation.The situation changes later in development. A method intended for validation, transfer, or long-term routine use carries much greater risk. Trial and error may identify a condition that appears satisfactory without revealing whether it represents the most robust region of the separation space. If that method later fails during transfer or routine operation, the consequences can be costly.For late-stage methods, the additional investment required to build a model can therefore reduce risk over the much longer lifetime of the analytical procedure.<h4>Models are only as good as the experiments behind them</h4>Chromatographic modeling is not automatic proof against poor method development. Ahmad compares the technology to a GPS: it is highly useful, but only if the analyst understands where they are trying to go.Careful experimental design remains essential. Incorrect dwell-volume or column-volume information, as well as insufficient column re-equilibration, can produce misleading predictions. Importantly, these problems may not be obvious when the experimental runs used to construct the model are compared with their calculated values. They may emerge only when the software begins predicting new conditions within the modeled design space.Modeling is also easier for some separations than others. Ahmad describes reversed-phase LC of small molecules as comparatively straightforward, while large biomolecules are more challenging because their retention can be highly sensitive to small changes in mobile-phase composition and temperature.Other separation modes present additional difficulties. Ahmad notes that his teams have found supercritical fluid chromatography (SFC) particularly challenging to model successfully. Fully autonomous method development remains another unresolved goal: current tools can automate parts of optimization, but analyst input is still required.<h4>Why two dimensions can reveal what one dimension cannot</h4>Ahmad’s early interest in 2D-LC came from polymer characterization. He explains the principle simply: one-dimensional chromatography may answer one question about a sample, while two complementary dimensions can answer two.Two copolymers, for example, may have similar molecular size and therefore coelute in size-exclusion chromatography. A second separation mechanism based on interaction with a stationary phase may distinguish them according to chemical composition. Combining both dimensions provides a more complete description of complex distributions involving molecular weight, composition, sequence, and other forms of heterogeneity.Historically, the complexity of both instrumentation and software limited wider adoption of 2D-LC. Ahmad recalls systems requiring several computers and multiple software packages. That situation has improved substantially, and 2D-LC is now easier to implement across research and pharmaceutical development.Nevertheless, modeling multidimensional separations still presents challenges. While models can be built for individual fractions or dimensions, current software does not easily identify a common optimum across multiple models simultaneously. Much of this comparison still requires manual work.<h4>Bringing sustainability into method development</h4>Another area where Ahmad sees room for change is green analytical chemistry.Traditional pharmaceutical method development has understandably prioritized resolution, sensitivity, robustness, and turnaround time. Sustainability has often been treated as a secondary consideration, particularly at the analytical scale, where solvent use appears small compared with preparative chromatography or manufacturing.That mindset is beginning to change. Ahmad argues that greenness metrics should increasingly be incorporated directly into method-development software. If analysts could immediately see how changes in flow rate, gradient conditions, solvent choice, or other parameters affect the environmental profile of a method, sustainability could become part of routine optimization rather than an assessment performed after the method has already been developed.The objective, however, is not to pursue the greenest possible method regardless of analytical performance. Ahmad emphasizes the need to develop methods that are as green as possible while remaining fit for purpose. Modeling can potentially help balance these competing requirements by identifying conditions that preserve separation performance while allowing greener solvents or alternative chromatographic conditions to be introduced.<h4>Advice for young chromatographers: do not treat HPLC as a black box</h4>For students learning chromatography, Ahmad’s central recommendation is straightforward: do not treat the HPLC system as a black box.Rather than collecting chromatograms by making random changes, he encourages analysts to build a mental model of the separation. When temperature, gradient, or another parameter is changed, they should ask why retention or selectivity changed and use each experiment to improve their understanding of the system.He also recommends making use of educational resources on chromatographic fundamentals and method development and, importantly, not being afraid to experiment with instrument conditions. Modern systems are robust, but the analyst still needs to understand the underlying separation science in order to use them effectively.The same proactive attitude applies to career development. Ahmad strongly encourages students to seek industry internships and make connections beyond their own institutions. Conferences and poster sessions can provide natural opportunities to meet scientists from industry, discuss research, and ask about internship opportunities. Based on his own experience working with undergraduate and graduate interns, these collaborations can benefit both students and research teams and may strongly influence later academic or career decisions.<h4>What still needs to be solved?</h4>Asked about the biggest unresolved challenges in separation science, Ahmad does not begin with technology. Instead, he points to a shortage of well-trained analytical chemists with a strong understanding of chromatographic fundamentals.Alongside education, he identifies several technical opportunities. One is truly autonomous method development, where an instrument and software could optimize a separation without continuous analyst input. Another is the ability to predict chromatographic selectivity directly from molecular structure.Such capability could be especially valuable in high-throughput discovery environments. If a laboratory could predict which stationary phase and conditions were most likely to separate a particular set of compounds or isomers before entering the laboratory, method development could become considerably faster and more efficient.Taken together, Ahmad’s perspective points toward a future in which chromatography becomes increasingly model-driven, automated, multidimensional, and sustainability-aware. But the technology does not eliminate the need for chromatographic knowledge. On the contrary, as analytical tools become more powerful, understanding the fundamentals may become even more important—the software can explore the separation space, but the scientist still needs to know what question to ask and how to interpret the answer.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

 In Silico Modeling, 2D-LC & Green Chromatography: Imad Ahmad on the Future of Method Development

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

Researchers from CATRIN at Palacký University, together with colleagues from VSB – Technical University of Ostrava and Charles University, have developed a new graphene ink for producing inexpensive and sensitive printed sensors. Using single-atom engineering, they incorporated copper atoms into the material, enabling highly sensitive detection of dopamine – a neurotransmitter responsible for transmitting signals between nerve cells. The approach, <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">presented in the journal Advanced Science</a>, has strong potential for use in medical diagnostics.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_8a02ad6a66.webp 117w," sizes="100vw" width="1920" height="2560">Single-atom engineering makes it possible to control the chemical reactivity of materials at the level of individual atoms. Its broader application in medical diagnostics has so far been limited by a lack of atomically engineered materials and printable inks based on them. Researchers have now developed a new class of single-atom-doped graphene materials as well as an ink for producing sensitive printed sensors. Copper proved particularly effective for dopamine detection, which is important for biomedical research and the development of advanced diagnostic technologies for diseases including Alzheimer’s disease.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_2_97e419f039.webp 117w," sizes="100vw" width="1920" height="2560">“The NGA-Cu ink we developed enables the production of fully inkjet-printed, sustainable paper-based electrodes at very low cost. Each sensor requires only 1.3 micrograms of active ink, costs approximately one Czech koruna and provided a stable response for at least three months. The ink is also fully compatible with commonly available materials inkjet printers. This creates significant potential for producing affordable, digitally designed sensors whose function can be tailored atom by atom,” said&nbsp;the corresponding author of the <a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">paper</a> Michal Otyepka.The basis for this new generation of single-atom inks was nitrogen-doped graphene acid (NGA), which CATRIN researchers developed several years ago. They have now progressively enriched it with different individual metal atoms, including copper, iron and manganese.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_3_c15d831792.webp 117w," sizes="100vw" width="1920" height="2560">“We found that the electrochemical response depends fundamentally on the type of atom used. While copper atoms significantly enhance the dopamine signal, other metals suppress it. What matters, therefore, is not only the presence of isolated metal atoms but also the specific metal involved. This is a very important finding for the further development of biosensors,” explained first author Martin-Alex Nalepa.The published results introduce a new class of sensing materials with considerable potential for medical diagnostics. The researchers present a broadly applicable platform for developing graphene inks containing individual metal atoms and open the way towards scalable, reproducible and material-efficient production of advanced electrochemical sensors.&nbsp;“We are only at the beginning and are fascinated by the new possibilities opening up before us. We can see that by changing the type of atom, we can control the sensitivity and selectivity of the sensor. In addition to dopamine, we can also determine immunoglobulins very precisely – antibodies whose levels and types may serve as important indicators of autoimmune or infectious diseases,” emphasised Radek Zbořil, a co-author of the paper.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_4_273cd760a5.webp 117w," sizes="100vw" width="1920" height="2560">Electrochemical biosensors can find applications not only in medicine but also in food quality control, drinking-water monitoring and environmental pollution monitoring. Their key components are functionalised electrodes, and graphene-based materials have proved particularly suitable for their production. A key factor in the success of the Olomouc researchers was the targeted preparation of graphene ink with the required properties.The research was supported by the TECHSCALE project under the Johannes Amos Comenius Programme.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg" alt="CATRIN: Atom by atom. Scientists reveal a new direction in medical diagnostics: Project TECHSCALE (No. CZ.02.01.01/00/22_008/0004587) is financed by the MEYS OP JAC Excellent research programme, supported by ERDF/ESF." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_CATRIN_Atom_po_atomu_Vedci_ukazali_novy_smer_v_lekarske_diagnostice_5_b1778f77f2.jpg 245w," sizes="100vw" width="620" height="87"><blockquote><h4>Article</h4><h4><a target="_blank" rel="noopener noreferrer" href="https://advanced.onlinelibrary.wiley.com/doi/10.1002/advs.76796">Single-Atom-Enhanced Fully Inkjet-Printed Electrochemical Sensor for Dopamine Detection</a></h4>Martin-Alex Nalepa, David Panáček, Vítězslav Hrubý, Matěj Jendřišák, Rostislav Langer, Michal Langer, Petr Jakubec, Ivan Dědek, Vojtěch Kupka, Michal Mazur, Radek Zbořil, Michal OtyepkaAdv. Sci., 2026, e76796<a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.1002/advs.76796">https://doi.org/10.1002/advs.76796</a><h6>licenced under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/">CC-BY 4.0</a></h6></blockquote><h4>Abstract</h4>Single-atom (SA) engineering offers atomic-level control over interfacial reactivity, yet the lack of printable SA-based inks hinders its practical translation into electrochemical sensing. Here, a fully water-based inkjet-printable ink is introduced based on nitrogen-doped graphene acid (NGA) hosting atomically dispersed Cu centers (NGA-Cu-ink). The ink enables digitally controlled, spatially defined deposition and fabrication of low-cost ($0.04 per sensor), fully inkjet-printed sustainable electrodes on paper. A comparison of NGA functionalized with different SA dopants (Cu, Mn, Fe, Ce) identifies a strong dopant-dependent electrochemical response, with Cu uniquely enhancing the analyte signal while other dopants suppress it, demonstrating that SA identity is a decisive design parameter in printed sensing interfaces profiling sensitivity and selectivity. The functional role of the NGA support is to provide dense anchoring sites (nitrogen and carboxylate groups) that stabilize atomically dispersed metal centers and create adsorption- and electron-transfer–active microenvironments. The NGA-Cu-ink yields enhanced dopamine oxidation, enabling quantitative detection on fully printed devices (limit of detection 9.7 µM; linear range 50–400 µM) and printed-on-electrode platforms (10.6 µM), while maintaining &lt;10% signal variation over 11 weeks. The approach establishes a general route to single-atom graphene-based inks for scalable, reproducible, and low-material-consumption manufacturing of advanced electrochemical sensors.

Atom by atom. Scientists reveal a new direction in medical diagnostics

Article

Academy

Scientists

Interview

Science and research

Video

The Czech Advanced Technology and Research Institute (CATRIN) at Palacký University is a cutting-edge scientific hub dedicated to advancing research in the fields of nanotechnology, biotechnology, and biomedicine. At its core, CATRIN boasts outstanding scientific teams, featuring international researchers. We emphasize interdisciplinarity, foster global collaborations, and work to translate our research findings into practical applications.

CATRIN

Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Separation Science

MSACL

Agilent Technologies

SelectScience

Wiley

Waters Corporation

You have the choice. PQ or MQ? What do you go with? Today all models exist in the labs from 100% PQ to 100% MQ. Some companies have a hybrid approach and also the frequency of the MQs can vary. The discussion will highlight some advantages and disadvantages of both approaches and give you some insights on which approach could work best for your lab.
<blockquote>
Presenter: Karen Krauel-Göllner, PhD (Product Manager Dissolution, Agilent Technologies, Inc.)
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<blockquote>
Karen Krauel-Göllner is a registered pharmacist and holds a PhD in Pharmacy from the University of Otago, New Zealand. Karen has worked in various roles in the field of pharmaceutical development. For the last 5 years she has been working with Agilent Technologies and currently holds a position as product manager for dissolution systems.
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<blockquote>
Presenter: Ken Boda (Dissolution Product Specialist, Agilent Technologies, Inc.)
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Ken joined Varian (now Agilent) in 2005 as an Applications Engineer and is now a Dissolution Product Specialist. Prior to working at Agilent, he worked in pharma specializing in dissolution testing and method development. As a Dissolution Product Specialist, Ken works with customers in determining the best systems for their needs and optimizing their work flows. He has also helped educate customers on dissolution science through webinars, the Dissolution Discussion Group, LinkedIn, and other channels.
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<blockquote>
Presenter: Bryan Crist (President, DissoAssist Consulting)
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Bryan is currently the president of DissoAssist consulting based in Wilmington, NC since 2021. He provides consulting, coaching, and training services for dissolution and drug release in the pharmaceutical industry, specifically, the areas of product and method development and validation, technology transfer, regulatory and compendial compliance, automation, troubleshooting, aberrant data investigation, apparatus and instrument qualification, SOP development, and laboratory audits.
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Previously, Bryan was a Scientific Affairs Manager with Agilent Technologies located in Cary, North Carolina, USA. His activities from 1997 until 2021 had focused on providing method and technical application support for dissolution and drug release testing in the pharmaceutical industry as well as working with VanKel, Varian and eventually Agilent R&amp;D, Marketing and Sales teams. Prior to 1997, Bryan performed analytical testing, supervised, and managed pharmaceutical laboratories with Bristol-Myers Squibb, Merck, AAI and Abbott Laboratories until he joined the dissolution equipment manufacturer VanKel.
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<blockquote>
Bryan has developed and routinely conducts numerous seminars related to dissolution and drug release testing. He’s provided numerous talks at exhibitions and conferences and has published numerous articles in journals and book chapters on dissolution related techniques and topics. He received his B.S. degree in Chemistry from Atlantic Christian College in Wilson, North Carolina. Bryan has been a former member of the USP Biopharmaceutics Expert Committee and is presently a member of several USP Expert Panels on Performance Verification Testing, Capsules, Performance Tests for Semisolid Dosage Forms and Solubility Criteria for Veterinary Drugs. He’s a Steering Committee member of the AAPS In-Vitro Release Dissolution Testing (IVRDT) Community Leadership Team, co-founder and co-moderator of the Dissolution Discussion Group (DDG) and is a current member of the American Chemical Society.
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Presenter: Vivian Gray (Founder VA Gray Consulting; Managing Director of Dissolution Technologies)
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<blockquote>
Vivian has spent the last 40+ years involved in all aspects of dissolution testing and evaluating new dissolution technology. At the United States Pharmacopeia, she enjoyed a long career serving first as a bench chemist, supervisor and lastly as a liaison to various expert USP committees, including the Biopharmaceutics and Dissolution Expert Committee. In 1997, Vivian joined the DuPont-Merck Pharmaceuticals Company Analytical Research and Development Section as the Head of the Dissolution Group.
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Vivian has been an invited speaker for conferences on 70 occasions (28 of these were international invitations), lecturing especially in the areas of dissolution method development and validation, FDA requirements in dissolution testing, troubleshooting dissolution method problems, and new dissolution technology and dosage forms. She is a proactive participant in the field as demonstrated by 54 publications, including 6 book chapters. Vivian has co-authored a book on dissolution testing called “Handbook of Dissolution Testing”, Third Edition, published in 2004.
</blockquote>

Dissolution Qualification – the PQ vs. MQ debate: What’s Right for Your Lab?

The discussion will highlight some advantages and disadvantages of PQ and MQ approaches and give you some insights on which approach could work best.

Dissolution Qualification – the PQ vs. MQ debate

Accelerating Routine Food &amp; Beverage Testing with Optimized Methods
Explore the latest technology and methodology solutions to help you deliver results more easily and efficiently for routine Food and Beverage QC testing for : Sugars, Sugar Substitutes Additives, Vitamins and Amino Acids.
<h3>EPISODE 4 - Amino Acid Analysis</h3>
<h3>Agenda</h3>
<h4>Getting Started with Amino Acid Analysis for Food Protein Hydrolysates and Cell Culture Media</h4>
<ul>
<li>Presenter: To be announced​</li>
</ul>
Abstract: Available soon
<h5>Live Q&amp;A session</h5>
<ul>
<li>Moderator: Cecile Pinto (Food &amp; Environmental Market Development Manager, South Europe - Waters)</li>
</ul>

Analysis of Amino Acid Analysis for Food Protein Hydrolysates and Cell Culture Media

Topics: Getting Started with Amino Acid Analysis for Food Protein Hydrolysates and Cell Culture Media

AAA for Food Protein Hydrolysates and Cell Culture Media

Aqueous size exclusion chromatography (SEC) of polymers can be quite complex. Depending on the type of sample, your choice of column chemistry and mobile phase conditions will be important for maintaining the size-exclusion mechanism.
In this webinar, we will highlight available column chemistry options for neutral, anionic, and cationic polymers. Advice for selecting your SEC column and polymer standards will be given with a particular focus on molecular weight range and optimizing for resolution. The impact of pH and buffer concentrations when preparing aqueous mobile phases is significant, and will also be discussed. Application examples will be featured.
<blockquote>
Presenter: Jean Lane (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.
</blockquote>

Agilent Technologies: Aqueous Size Exclusion Chromatography of Water-Soluble Polymers: Why Column Choice and Method Conditions Matter

Aqueous Size Exclusion Chromatography of Water-Soluble Polymers: Why Column Choice and Method Conditions Matter

Column chemistry options for neutral, anionic, and cationic polymers.

Aqueous SEC (Chromatography) of Water-Soluble Polymers

Traditional QuEChERS clean-up of food and agricultural sample matrices for pesticide residue analysis relies on the dispersive solid phase extraction (dSPE) technique.
For analytical laboratories using dSPE workflows, the sample is shaken with dispersed SPE media to remove unwanted matrix components that negatively impact data quality. In this webinar, we will present a new technique that simplifies QuEChERS clean-up and enhances analytical performance when compared to traditional dSPE. ISOLUTE® cSPE for QuEChERS columns allow for a convenient, flow-through clean-up workflow for both AOAC and EN QuEChERS techniques. This new workflow eliminates several time-consuming processes, such as capping, shaking, de-capping, and sample transfer. In addition to labor reduction, the ISOLUTE® cSPE for QuEChERS process is more effective at removing unwanted matrix components. Compared to traditional dSPE, extracts processed using cSPE columns result in cleaner analytical baselines, making sample analysis easier, more accurate, and more reliable.
By attending this presentation, you will learn:
<ul>
<li>How to enhance analytical performance, making sample analysis easier, more accurate, and reliable.
</li>
<li>How to simplify sample preparation workflows by eliminating processing steps.
</li>
</ul>
Who should attend:
<ul>
<li>Analytical laboratories performing chemical residue analysis such as pesticides, fungicides, and insecticides.
</li>
<li>Laboratories testing food, agricultural, and cannabis samples.
</li>
<li>Lab bench, sample preparation, and extraction chemists.
</li>
<li>Lab analytical chemists performing gas and liquid chromatography with mass spectrometry (GCMS &amp; LCMS).
</li>
</ul>
<blockquote>
Presenter: Evan Walters (MSc) (Market Segment Manager, Biotage)
</blockquote>
<blockquote>
Evan Walters is passionate about science and analytical chemistry in environmental, food, and agriculture markets. A trained environmental chemist and geochemist, Evan uses his background in analytical chemistry to connect laboratories with products and workflow solutions to ensure their success. Evan received a Master of Science in Geochemistry from Southern Illinois University and is a published scientific author.
</blockquote>

Separation Science: Improving pesticide residue analysis following AOAC and EN methods with a new QuEChERS cSPE cleanup technique

Improving pesticide residue analysis following AOAC and EN methods with a new QuEChERS cSPE cleanup technique

New technique that simplifies QuEChERS clean-up and enhances analytical performance when compared to traditional dSPE.

Pesticide residue analysis & new QuEChERS column SPE cleanup

Peptide mapping for biotherapeutic proteins relies on protein digestion which includes the enzymatic treatment of a protein to produce peptide fragments to further characterize specific peptide regions and modifications.
The results of protein digests are essential for protein structural characterization, protein identification, and protein modification monitoring including post-translation modifications, product related impurities, and multi-attribute monitoring (MAM) methods. However, sample preparation for peptide mapping is complex, laborious and often with long digestion times.
In this webinar, we will discuss an automatable sample preparation protocol for peptide mapping that provides a reproducible tryptic protein digest developed for regulated and research peptide mapping applications.
<blockquote>
Presenter: Leslie Napoletano (Principal Product Marketing Manager, Consumables &amp; Lab Automation-Biopharm, Waters Corporation)
</blockquote>
<blockquote>
Leslie has been with Waters since 2012 providing support and education to scientists in New Jersey with Waters chemistry consumables including sample preparation, column selection and delivering educational seminars to customers. More recently in 2021, Leslie has moved into the Product Marketing group focusing on bringing on new consumable products for protein characterization. Prior to joining Waters, Leslie worked as a scientist in protein characterization in the pre-clinical development of monoclonal antibodies at Merck and prior to that at Teva as a R&amp;D chemist.
</blockquote>

Waters Corporation: Improved Peptide Mapping of Biotherapeutics

Improved Peptide Mapping of Biotherapeutics

Automatable sample preparation protocol for peptide mapping that provides a reproducible tryptic protein digest.

Wiley’s KnowItAll spectral software offers solutions to identify, analyze, and manage analytical data (IR, MS, NMR, Raman, and UV-Vis). Combined with Wiley’s comprehensive, high-quality spectral reference libraries, it provides an unparalleled solution for fast, reliable spectral analysis.
With the KnowItAll 2024 software release, Wiley continues to expand KnowItAll’s capabilities, including an integrated tool for multi-technique quantitative analysis that can greatly streamline your workflow; plus, many enhancements and upgrades to data management, processing, and reporting. In this release, we have also made advances to our NMR workflow and LC-MS databasing.
In this webinar, we will review the latest features and how they can impact your research and analyses.
<blockquote>
Presenter: Dr. Michelle D’Souza, Ph.D. (Product Manager, Software Products, Wiley Science Solutions)
</blockquote>
<blockquote>
Dr. D’Souza brings years of insight and knowledge in the area of spectral informatics with leadership roles at Wiley and Bio-Rad Laboratories. In addition to multiple years as a scientific researcher at Abbott (now AbbVie) and DuPont. She has a Ph.D. in Chemistry from Northern Illinois University and a Masters in Analytical Chemistry form the Chinese Academy of Preventative Medicine. She earned a BS degree from the Chemistry Department of Beijing University.
</blockquote>

What's New in KnowItAll 2024

The latest features and how they can impact your research and analyses.

LC-MS has been used for biopharmaceutical analysis for over 20 years and, in recent years, the demands of the industry have expanded significantly for this technique. This is due to drug classes expanding from monoclonal antibodies (mAbs) to include oligonucleotide and mRNA therapies, as well as novel protein-based drugs.
This webinar series explores the pivotal role of LC-MS in biopharmaceutical development, outlining the use of LC-MS for host cell protein impurity analysis, the capabilities of the ZenoTOF 7600 system for protein characterization, and the application of LC-MS in oligonucleotide and mRNA lipid nanoparticle analysis. These webinars provide crucial insights into the evolving landscape of LC-MS technology, highlighting it as an indispensable tool for ensuring the safety, efficacy, and purity of the next generation of life-saving drugs.
<h4>Session 2: Advancing protein biotherapeutics characterization with cutting-edge LC-MS</h4>
At the core of Leanbio&#39;s operations is the ZenoTOF 7600 system, a mass spectrometry (MS) platform for comprehensive protein characterization, designed to provide critical support for process development during manufacturing. This tool helps to determine the exact molecular weight and to identify product variants through intact mass analysis. It also supports sequence confirmation, analysis of post-translational modifications (PTMs), investigation of glycopeptides, and determination of disulfide bond assembly via peptide mapping.
In this webinar, discover more about the ZenoTOF 7600 system, and learn how Leanbio Pro provides the biopharmaceutical industry with analytical tools and expertise, contributing to a deeper understanding of intricate protein structures and their significance in therapeutic development.
<blockquote>
Presenter: Thomas Kofoed, PhD (Co-founder and CEO, Alphalyse)
</blockquote>
<blockquote>
Dr. Thomas Kofoed holds a PhD in Organic Chemistry and a BSc in Business Administration. He has over 15 years of management experience in the biotech industry and has set up business relationships with hundreds of biopharmaceutical companies. As CEO of Alphalyse, his main focus is putting together a diverse team of highly skilled employees creating an innovative environment focusing on quality and customer needs.
</blockquote>
<blockquote>
Presenter: Dr. Angela Fernandez (Mass Spectrometry Team Leader &amp; Project Manager, Leanbio Pro)
</blockquote>
<blockquote>
Dr. Angela Fernandez has a PhD in Biochemistry and Molecular Biology, and over a decade of experience in research and development in the biopharmaceutical industry and academia. Her expertise working in contract research organization (CRO) and CDMO companies includes biophysical and biochemical techniques for protein characterization, mass spectrometry-based analysis of new biological entities, and a comprehensive knowledge of drug development processes in a regulatory environment. Dr. Fernandez has established a new mass spectrometry platform from inception, expanding Leanbio&#39;s service offerings.
</blockquote>
<blockquote>
Presenter: Stephen Lock, PhD (Senior Market Development Manager, Biopharma, SCIEX™)
</blockquote>
<blockquote>
Dr. Stephen Lock obtained his PhD in Physical Organic Chemistry from the University College of Swansea and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX™ in various technical roles for over 25 years, and is currently the EMEAI BioPharma Senior Market Development Manager. He is also an Adjunct Professor at the University of Pannonia where he acts in a volunteer role as a Scientific Advisor for the Horváth Csaba Memorial Laboratory, where he provides technical advice and supports the group’s fundamental research and development of new applications for capillary electrophoresis.
</blockquote>
<blockquote>
Presenter: Dr. Carrie Haslam (Associate Editor, SelectScience)
</blockquote>
<blockquote>
Dr. Carrie Haslam is an Associate Editor at SelectScience, playing a key role in content production and specializing in Materials Science, Alzheimer’s disease and Clinical Diagnostics. Carrie completed a Ph.D. from The University of Plymouth, where she developed graphene-based biosensors for the early diagnosis of Alzheimer’s disease.
</blockquote>

SelectScience: Mastering LC-MS in biopharmaceutical analysis - Session 2: Advancing protein biotherapeutics characterization with cutting-edge LC-MS

Advancing protein biotherapeutics characterization with cutting-edge LC-MS

Protein biotherapeutics characterization with LC-MS

Do you need to free up your time in the laboratory? Want to use your ÄKTA™ system to purify samples at a higher throughput while maintaining consistency and accuracy?
In this webinar, find out how to improve your chromatography workflows and get ready to automate. Dr. Martin Sichting and Lisa Grufman from Cytiva will discuss how ÄKTA systems can be automated to make your purification process efficient, easy, and reliable.
Key learning objectives
<ul>
<li>Learn to combine chromatography steps and run parallel purifications
</li>
<li>Explore affinity chromatography and size exclusion
</li>
<li>Discover how to automate purifications
</li>
</ul>
Who should attend?
<ul>
<li>Life science researchers/scientists working with chromatography to purify proteins/molecules.</li>
</ul>
<blockquote>
Presenter: Dr. Martin Sichting (Cytiva)
</blockquote>
<blockquote>
Dr. Martin Sichting has a PhD in biochemistry and is an ÄKTA expert by profession. He joined GE/Cytiva in 2008 as an ÄKTA trainer. He continued his career as ÄKTA Application Specialist and Chromatography Application Leader before taking on his current role as Senior Product Manager in the Lab ÄKTA team. He is committed to advancing the fields of biochemistry and chromatography.
</blockquote>
<blockquote>
Presenter: Lisa Grufman (Cytiva)
</blockquote>
<blockquote>
Lisa Grufman is a seasoned professional in the life science industry, and is currently working as the Global Product Manager for Lab ÄKTA chromatography systems at Cytiva. Besides being well-versed in immunoassay diagnostics, she has also substantial knowledge of ÄKTA systems and is skilled in simplifying chromatography.
</blockquote>
<blockquote>
Presenter: Sofia Hamadache (SelectScience)
</blockquote>
<blockquote>
Sofia studied Chemistry at University College London, UK. As a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.
</blockquote>

SelectScience: How to master automation for your chromatography workflows

How to master automation for your chromatography workflows

How to improve your chromatography workflows and get ready to automate.

This presentation will introduce the instrument features on the ZenoTOF 7600 system that provide qualitative flexibility combined with quantitative power for NPS detection and characterization.
The depth of information extracted from EAD-based MS/MS spectra combined with the improved MS/MS sensitivity were leveraged for characterization of structurally related isomeric species while providing detection of low-level potent NSO and metabolites in authentic case samples. The presentation will demonstrate that these new technological advancements on the system can be leveraged to provide more confidence in the quantified amounts of drugs and metabolites detected in discarded authentic case samples which is critical when determining the cause of death following an accidental overdose.
<blockquote>
Presenter: Pierre Negri, B.S, Ph.D (SCIEX)
</blockquote>

SCIEX: Enhancing high-resolution mass spectrometry performance for NPS analysis with improved sensitivity and characterization

Enhancing high-resolution mass spectrometry performance for NPS analysis with improved sensitivity and characterization

High-resolution mass spectrometry for NPS analysis

Accelerating Routine Food &amp; Beverage Testing with Optimized Methods
Explore the latest technology and methodology solutions to help you deliver results more easily and efficiently for routine Food and Beverage QC testing for : Sugars, Sugar Substitutes Additives, Vitamins and Amino Acids.
<h3>EPISODE 4 - Amino Acid Analysis</h3>
Amino acid testing is a high-volume assay for many food and dietary supplement companies, and is needed for infant formula, animal feed, sports nutrition products, and dietary supplement labeling. <a href="https://lcms.cz/products/205">The Waters AccQ-Tag™ Ultra Derivatization kit</a> is the industry standard for amino acids analysis and is included in AOAC Official Method 2018.16 for infant formulas, adult nutrional&#39;s, dairy and cereals. While kits make analyses easier, it is important to consider which sample preparation tecniques and LC systems are best fit for your specific needs
This presentation will include:
<ul>
<li>An overview of the Waters AccQ-Tag Ultra Derivatization kit for amino acid analysis
</li>
<li>Sample preparation for free and bound amino acids in various matrices
</li>
<li>HPLC and UPLC™ options for separation and detection
</li>
<li>Increased workflow efficiency with the <a href="https://lcms.cz/products/208">Andrew+Pipetting Robot</a>
</li>
</ul>
Guest Speaker Presentation summary:
Amino acids are key nutrients and building blocks for all proteins. They need to be provided in adequate quantities through food to ensure proper growth and to maintain good health. Amino acid profiling helps us ensure the high quality of products such as infant formulas, nutritional products, dairy products, and cereals. A standardized method is needed to ensure consistent and comparable results across the many different labs that perform amino acid profiling. In this presentation, I will share the standardization journey of our total amino acid method based on pre-column derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC), and discuss next steps to improve method throughput.
Live Q&amp;A session
<blockquote>
Moderator: Cecile Pinto (Food &amp; Environmental Market Development Manager, South Europe - Waters)
</blockquote>
<blockquote>
Presenter: Christophe Fuerer (Senior Specialist Proteins Analysis, mass spectrometry, analytical chemistry &amp; Proteins, Société des Produits Nestlé SA)
</blockquote>
<blockquote>
Manage the Nestlé portfolio of analytical protein methods, evaluate proficiency tests, develop andvalidate analytical methods, liaise with standardization bodies, provide expert assistance to qualityassurance centers, R&amp;D centers, and factories, supervise lab technicians, and coordinate lab work.
</blockquote>
<blockquote>
<ul>
<li>Mass Spectrometry</li>
<li>Intact protein analyses (proteoform fingerprinting and quantification) by UHPLC-HRMS, with anemphasis on the detection of genetic variants and protein glycation</li>
<li>Multiple Reaction Monitoring (MRM) methods for absolute quantification of trace allergens infood matrices and milk proteins in dairy matrices</li>
<li>Method development and validation</li>
<li>Intact protein fingerprinting, detection, and quantification of milk proteins by UHPLC-HRMS</li>
<li>Amino acids in food matrices by UHPLC-UV</li>
<li>Whey protein content by capillary gel electrophoresis</li>
<li>Trace amounts of intact proteins by gel electrophoresis</li>
<li>Method standardization and active contribution to standardization bodies</li>
<li>AOAC International</li>
<li>Expert Review Panel on Nutrients (2022-present, member)</li>
<li>Total Amino Acids by UHPLC-UV (Final Action 2018.06, author)</li>
<li>Whey protein content in infant formula by SDS-CGE (Final Action 2016.15, author)</li>
<li>A1- and A2-type beta casein (SMPR 2022.005), Lactoferrin (SMPR 2020.005), and Food allergens by MS (SMPR 2016.002) (working group member)</li>
<li>IDF/ISO</li>
<li>Total Amino Acids by UHPLC-UV (IDF 254, ISO 4214, author)</li>
<li>Whey protein content by SDS-CGE (IDF 247, ISO 23293, author)</li>
<li>Evaluation of nitrogen conversion factors (IDF Bulletin, co-author)</li>
<li>USP</li>
<li>Expert Panel on Dietary Proteins (2018-present, member)</li>
<li>Detection of foreign proteins in whey (FCC, Appendix XVI, author)</li>
<li>CEN</li>
<li>TC454/WG7 - Algae and algae products, Product test methods - saccharides, proteins,amino-acids (working group member)</li>
</ul>
</blockquote>
<blockquote>
Presenter: Kimberly Martin (Evaluation Scientist II, Waters Corporation)
Kimberly Martin is part of the QA/QC Strategy Group at Waters Corporation and serves as an Evaluation Scientist II that verifies, validates and tests new instrumentation and products. She has been with the company for 5 years. She has a total of 14 years of analytical chemistry experience including polymer chemistry, food and environmental chemistry, and pharmaceutical chemistry. Kimberly has extensive experience in HPLC, LCMS, GCMS, GC-FID, additional MS technologies and sample preparation.
</blockquote>
<blockquote>
Presenter: Don Trinite (Senior Principal Technical Support Specialist, Waters Corporation)
Don Trinite has been working at Waters Corporation for thirty-five years. Don is a Senior Principal Technical Support Specialist for Waters Technologies Corporation for the last twenty-nine years. He is involved in developing seminars for training users in methods development and methods transfer and troubleshooting for HPLC, UPLC, IC, GPC, Prep LC and UPC² ™. He is a key person in amino acid applications development and support. Recently, he has been involved with theAndrew+™ Robotic system, testing protocols for various amino acid sample protocols to automate different aspects of sample handling for these types of samples. He is also involved in troubleshooting for Data Handling and Networking Systems. Don has also developed methods for users and has had several application notes published. Prior to this, Don was in the field service organization for Waters as Senior Service Specialist and Service Manager for five years. He was previously employed in the chemical industry by UOP, Des Plaines, Illinois, in a chromatography service group supporting chemical synthesis, production and natural product research as a Senior Research Technician. Don has a total of 43 years of experience in the LC field.
</blockquote>

Waters: Analysis of Amino Acid Analysis for Food Protein Hydrolysates and Cell Culture Media

Improving pesticide residue analysis following AOAC and EN methods with a new QuEChERS cSPE cleanup technique

How to master automation for your chromatography workflows

Analysis of Amino Acid Analysis for Food Protein Hydrolysates and Cell Culture Media

Analysis of Amino Acid Analysis for Food Protein Hydrolysates and Cell Culture Media

Advancing protein biotherapeutics characterization with cutting-edge LC-MS

Improved Peptide Mapping of Biotherapeutics

Aqueous Size Exclusion Chromatography of Water-Soluble Polymers: Why Column Choice and Method Conditions Matter

Dissolution Qualification – the PQ vs. MQ debate: What’s Right for Your Lab?

What's New in KnowItAll 2024

Enhancing high-resolution mass spectrometry performance for NPS analysis with improved sensitivity and characterization

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