What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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<a href="https://lcms.cz/webinars?search=DIA">Data-independent acquisition (DIA)</a> allows for suspect screening in complex matrices with retroactive analysis capabilities important to emerging compounds of interest in food safety. However, with increased analyte scope, the review of questionable identifications can create an analysis burden requiring timely review and reinjections.
Here, we present a robust DIA methodology with a novel prototype LC/Q-TOF, leveraging a combination of high-resolution, extended dynamic range, stable and accurate mass, and isotopic fidelity with coeluting fragmentation ions for identification, analyzed with MH Quantitative Analysis 12.1 and LC Screener Tool. Over 200 analytes were identified from a spiked broccoli matrix prepared with QuEChERS, resulting in good calibration curve linearity and low area %RSDs. Fused is the Intelligent Reflex Workflow used to automatically evaluate and reinject samples in which results were flagged as questionable and/or identified via targeted MS/MS utilizing the available m/z and RT information.
Transferring questionable results into confirmed identifications is achieved by acquiring targeted data for present compounds. Leveraging the combination of DIA and automatically enabled targeted MS/MS reinjections via Intelligent Reflex allows for broad screening analysis and confident identification confirmation of analytes.
These presentations were recorded during <a href="https://lcms.cz/webinars?search=ASMS">ASMS 2023</a> so if you weren’t able to attend in person there’s a chance to watch them on-demand. The presentations will be followed by a Q&amp;A so please remember to submit your questions by completing the registration form and they will be considered by the presenters.
By attending this presentation, you will learn key strategies for:
<ul>
<li>Learning robust and reliable screening methods for pesticides in food</li>
<li>Generating high-quality data</li>
<li>Keeping up with current and future regulations</li>
<li>Simplifying workflow solutions</li>
</ul>
Who Should Attend:
<ul>
<li>Laboratory Directors in the food industry</li>
</ul>
Start times:
<ul>
<li>Broadcast #1: 7 February – starting at 10 am GMT (London) / 11 am CET (Paris/Berlin)</li>
<li>Broadcast #2: 7 February – starting at 10 am PST (Los Angeles) / 12 pm CST (Chicago) / 1 pm EST (New York)</li>
</ul>
Duration: Approximately 60 minutes
<blockquote>
Presenter: Cate Simmermaker (LC/MS Applications Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Cate Simmermaker is an LC/MS application engineer with Agilent Technologies working primarily with high-resolution mass spectrometry in small-molecule analysis. Cate has worked in applications including metabolomics, food safety, and forensics on Q-TOF and QQQ platforms. She completed her PhD in chemistry at the University of the Pacific and received a Master&#39;s in biochemistry from San Francisco State University.
</blockquote>
<blockquote>
Presenter: Christian Klein (LC/Q-TOF Portfolio Product Manager, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
With an education in chemistry, Chris Klein received his PhD in 2005 at the Max Planck Institute for Biochemistry in Martinsried, Germany. He joined Agilent Technologies in 2010 as the lead chemist in the LC/Q-TOF R&amp;D team. In 2018, Chris joined the marketing team as Portfolio Product Manager, responsible for LC/Q-TOF and ion mobility.
</blockquote>

Separation Science: From Screening to Confirmation: A Comprehensive Food Safety Analysis

From Screening to Confirmation: A Comprehensive Food Safety Analysis

A robust DIA methodology with a novel prototype LC/Q-TOF, with high-resolution, extended dynamic range, stable and accurate mass, and isotopic fidelity.

From Screening to Confirmation: Food Safety Analysis

Introducing a new bioanalytical technology for assessing monoclonal antibody (mAb) sample quality from early-stage candidate selection to manufacturability evaluation - mass photometry.
In this webinar, you’ll discover how mass photometry can be used for forced antibody degradation assessment, a crucial aspect of research and development for recombinant mAb therapeutics. Our expert speaker will also demonstrate how mass photometry data compares to and complements the current gold standard, size exclusion chromatography (SEC), as well as providing insights into other applications of mass photometry in therapeutic antibody R&amp;D labs.
Attend this webinar to discover:
<ul>
<li>How mass photometry enables rapid assessment of sample quality</li>
<li>How mass photometry complements other analytical methods for antibody sample characterization, such as SEC</li>
<li>What the sample requirements are for detection of antibody aggregates and fragments</li>
</ul>
<blockquote>
Presenter: Dr. Amy Chau (Field Application Specialist, Refeyn)
</blockquote>

Technology Networks: Advancing mAb Stability Studies With Mass Photometry

Advancing mAb Stability Studies With Mass Photometry

Discover how mass photometry can be used for forced antibody degradation assessment

Attendees will see how currently available automated synthesis equipment can provide major time savings, improve workflow productivity, and enable you to complete more projects.
This webinar compares automated synthesis workflow using modern lab equipment such as a microwave reactor, flash chromatography system, recyclable reaction work-up tools, and a high-speed solvent evaporator to a manual workflow using hot plates or refluxing, glass column chromatography and rotary evaporators.
Both a general comparison and a real synthetic workflow are presented.
<blockquote>
Presenter: Bob Bickler (Senior Technical Specialist, Biotage)
</blockquote>
<blockquote>
Bob Bickler is the Senior Technical Specialist at Biotage and has over 45 years of chromatography experience. During his career, he has worked for Whatman, YMC, Waters, and for the past 25 years at Biotage. Bob has designed and launched many products including Biotage SNAP columns, SP1, Isolera LS, Isolera Prime Flash System, as well as several accessories.
</blockquote>

Biotage: How to Improve Small Molecule Synthesis Workflow by > 70%

How to Improve Small Molecule Synthesis Workflow by > 70%

See how currently available automated synthesis equipment can provide major time savings, and improve workflow productivity

Traditional UV/Vis spectroscopy uses fixed pathlengths to measure different analytes, such as nucleic acids, proteins, and cells from biological samples. These samples are often limited in volume or highly concentrated, which presents various challenges.
The CTech™ SoloVPE® System uses patented variable pathlength technology (VPT) to accurately measure the targeted concentration of various analytes in less than 2 minutes. The system can measure concentrations without being dependent on sample volume or sample concentration, removing any dilution requirements. 
In this webinar, we will review VPT and how it can be used in various applications from protein to nucleic acid therapeutics. We will share real-world case studies demonstrating how companies have integrated the SoloVPE System into their manufacturing workflow for release and in-process testing.
<h5>Key Learning Objectives:</h5>
<ul>
<li>The science of variable pathlength spectroscopy and why variable pathlength provides better accuracy than traditional fixed pathlength methods
</li>
<li>Overview of the SoloVPE System and the benefits: 
<ul>
<li>No sample dilutions</li>
<li>Guaranteed linear results </li>
<li>No baseline correction</li>
<li>Testing of low and very high concentration samples from 0.01 mg/mL up to 300mg/mL (MAbs) </li>
<li>Implementing in the analytics/QC lab or in the production suite for at-line testing (IPT)</li>
</ul>
</li>
<li>Review SoloVPE implementation in multiple applications - quantitate proteins, nucleic acids and excipient analysis, such as polysorbate and free sulfhydryl concentrations
</li>
</ul>
<h5>Who Should Attend:</h5>
<ul>
<li>Process Development Scientist </li>
<li>Analytical Development Scientist </li>
<li>Quality Control, Quality Assurance Scientist</li>
<li>Manufacturing Science and Technology (MSAT) Scientist</li>
</ul>
<blockquote>
Presenter: Joe Ferraiolo (Director, Bioanalytics Applications, Repligen)
</blockquote>
<blockquote>
Presenter: Catherine Dold (Health &amp; Environment Writer, C&amp;EN Media Group)
</blockquote>

C&EN: Variable Pathlength Spectroscopy for Dilution-Free Concentration Measurement in GMP Environments

Variable Pathlength Spectroscopy for Dilution-Free Concentration Measurement in GMP Environments

We will review VPT and how it can be used in various applications from protein to nucleic acid therapeutics.

VPS for Dilution-Free Concentration Measurement

The development momentum for RNA modalities has accelerated with the recent approvals of mRNA-based vaccines and RNA(CRISPR)-based gene therapies. These approvals have highlighted the need for the development of robust analytical methods for monitoring the relevant critical quality attributes (CQAs) of RNA molecules to ensure proper control of manufacturing processes and product quality. Oligonucleotide mapping via liquid chromatography coupled to mass spectrometry (LC-MS) has now been established as an effective tool for RNA CQA assessment, however, the limited tool sets for efficient acquisition and processing of the LC-MS data from an RNA digest have presented a time-consuming challenge to the innovators bringing these early molecules to market.
In this webinar, we present a workflow for RNA CQA analysis developed on the waters_connect informatics platform. Data acquired on this compliant-ready platform was analyzed using the INTACT Mass application as the engine for data processing and producing RNA fragment assignments, assisted by two external microApps: mRNA Cleaver (to generate in silico fragments for targeted search), and Coverage Viewer (to visualize digestion data coverage). We will demonstrate these tools with application to CQA analysis of both sgRNA (ID and Structural Fidelity) and mRNA (ID, Structural Fidelity, 5’Capping, 3’ PolyA Tail A) molecules. 
What you will learn from this presentation:
<ul>
<li>A workflow for acquisition and data analysis from RNA digestion fragment mapping studies.</li>
<li>Application of this workflow to sgRNA and mRNA CQAs.</li>
<li>Discussion of the challenges and opportunities deploying these methods for routine analysis, including the use of the BioAccord LC-MS System and waters_connect informatics to make MS based RNA analysis accessible to non-MS experts.</li>
</ul>
Who should attend?
<ul>
<li>Scientists, Technicians and Managers, who are charged with analyzing therapeutic RNA samples.</li>
<li>Others from pharmaceutical and contract organizations looking towards developing and commercializing these molecules, who want to better understand the analytical challenges of producing high quality products and processes.</li>
</ul>
<blockquote>
Presenter: Ying Qing Yu (Biopharmaceutical Program Science Team Leader, Waters Corporation)
</blockquote>
<blockquote>
Ying Qing is the Biopharmaceutical Program Science Team Leader at Waters Corporation. She joined Waters Corporation in 2001, shortly after she received her Ph.D. from the Analytical Chemistry Department at Purdue University. Her area of expertise is on analytical method development for various Biotherapeutics using UPLC/QTOF MS platform, including sample preparations, LC-MS optimization, and development of application specific software tools. Recently, her team has been taking on the challenges of developing LC-MS solutions for nucleic acid characterization.
</blockquote>

Waters: LC-MS for RNA Therapeutics to Ensure Product Quality and Process Consistency

LC-MS for RNA Therapeutics to Ensure Product Quality and Process Consistency

Workflow for RNA CQA analysis developed on the Waters_connect informatics platform.

LC-MS for RNA Therapeutics Product Quality

Attendees will see how currently available technologies can provide major solvent (and time) savings and improve their workflow and productivity.
<h5>This presentation provides easy to do suggestions to reduce organic solvent use including:</h5>
<ul>
<li>Step gradients</li>
<li>Smaller particle size columns</li>
<li>HILIC as replacement for DCM/MeOH</li>
<li>Reversed phase</li>
</ul>
<h5>Who should attend this webinar mini-series?</h5>
<ul>
<li>Small Molecule Organic Chemists</li>
<li>Medicinal Chemists</li>
<li>And their Managers</li>
</ul>
<blockquote>
Presenter: Bob Bickler (Senior Technical Specialist, Biotage)
</blockquote>
<blockquote>
Bob Bickler is the Senior Technical Specialist at Biotage and has over 45 years of chromatography experience. During his career, he has worked for Whatman, YMC, Waters, and for the past 25 years at Biotage. Bob has designed and launched many products including Biotage SNAP columns, SP1, Isolera LS, Isolera Prime Flash System, as well as several accessories.
</blockquote>

Biotage: Improve your Flash Chromatography Skills: Tips for Sustainable Purifications

Improve your Flash Chromatography Skills: Tips for Sustainable Purifications

Attendees will see how currently available technologies can provide major solvent (and time) savings and improve their workflow and productivity.

Better Flash Chromatography Skills: Tips for Purifications

Minimize risk, streamline workflows and improve the reliability of your analytical measurements.
How do the Alliance iS and Empower work together to reduce common human errors?
Empower 3.8.0 offers unique features that work specifically with Waters Alliance iS HPLC System and can reduce common made human errors.
Learn how to identify potential errors earlier, reduce the number of retests and failure investigations and register!
<blockquote>
Presenter: Arjan Timmerman (Principal Pharma Market Development Manager Pharmaceuticals, Compliance and Impurities)
</blockquote>
<blockquote>
Presenter: Andrea Gheduzzi (Principal LC Market Development Manager, EMEA)
</blockquote>
<blockquote>
Presenter: Carsten Fischer (Informatics Product Marketing, EMEA)
</blockquote>

Waters Corporation: Reducing Human Errors with Empower 3.8.0

Reducing Human Errors with Empower 3.8.0

Empower 3.8.0 offers unique features that work specifically with Waters Alliance iS HPLC System and can reduce common made human errors.

<h3>Biopharma Brilliance Webinar Series:</h3>
 Glycan &amp; Sialic Acid Workflows in Biotherapeutic Glycoproteins: Sample Prep to Analysis.
<h4>Session 1: Workflows for Released N-Glycans: Sample Preparation and Analysis</h4>
Glycosylation is considered one of the critical quality attributes of biomolecules. Therefore, there is a need to characterize, quantify, and determine if they are in acceptable ranges as part of the development process for a glycoprotein innovator, biosimilar or bio-better pharmaceutical.
In the race to discover the next promising biotherapeutic or develop a reliable biosimilar, one cannot compromise on analytical accuracy and efficiency. Reducing process development time, having the ability to make procedural changes quickly, and minimizing variability in analyses are just a few of the challenges faced every day.
 Join us for an engaging webinar to learn about these challenges and how to try to overcome them.
What will you learn?
<ul>
<li>Efficient preparation and analysis of released N-glycans from biotherapeutic glycoproteins.</li>
<li>Techniques to significantly reduce sample preparation time and enhance N-glycan analysis productivity.</li>
<li>Selection criteria for an appropriate HILIC column and mobile phase.</li>
<li>Achieving greater separation of charge groups with the new Agilent AdvanceBio Amide HILIC column.</li>
</ul>
Join both the session and get certified for Brilliance.
<blockquote>
Presenter: Dr. Rajni Mujoo (Technical Support Specialist, CSD, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Rajni is working with us as Technical Support Specialist in Chemistries and Supplies Division at Agilent Technologies since 2011. She has a Ph.D degree in Food Science from CFTRI, Mysore and Post Doctorate from Department Of Food Science, Michigan State University. She also has four International Presentations &amp; 13 publications in reputed National &amp; International journals to her credit. Rajni has a total work experience of 16 yrs spread across research and Sales building expertise in different Instrument techniques.
</blockquote>
<blockquote>
Presenter: Mr. Shreyas Tribhuvane (Business Development Manager, PL BC, Agilent Technologies)
</blockquote>
<blockquote>
Shreyas has 10 years experience in working closely with biopharma customers in understanding their analytical characterization needs. As a BDM for Biocolumns and Glycan kits his prime responsibility is to work on the Biopharma market needs and communicate with Agilent solutions unit team in order to have apt solutions. He has a hands-on experience on Agilent Glycan sample prep kits and helped customers in setting up these kits for their glycan characterization work.
</blockquote>

Agilent: Biopharma Brilliance - Session 1

Biopharma Brilliance - Session 1

Process development time, procedural changes, and variability in analyses are just a few of the challenges faced every day.

Parallel capillary electrophoresis is a powerful technique for automated protein analysis. The ProteoAnalyzer system is a novel instrument that uses this technique to overcome the limitations of traditional methods. It features 12 capillaries, validated reagents, and automated cleaning and rejuvenation after each run, enabling the user to obtain high-quality data for various protein sample types.
In this webinar, Dr. Kyle Luttgeharm, Product Manager and Dr. Gizem Bergdahl, Research Scientist, DAKO Denmark R&amp;D &amp; Manufacturing, will discuss how the ProteoAnalyzer system can transform protein analysis workflows.
Key learning objectives
<ul>
<li>Understand more about the ProteoAnalyzer</li>
<li>Learn how to improve the consistency of your protein analysis results</li>
<li>Explore how the ProteoAnalyzer can be used to analyze challenging sample types including total protein extracts and crude cell lysates</li>
</ul>
Who should attend?
<ul>
<li>Anyone who needs help to gain laboratory efficiency, free up technician time, and minimize delays due to ambiguous results leading to re-sample analysis.</li>
</ul>
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.
<blockquote>
Presenter: Dr. Kyle Luttgeharm (Product Manager, Agilent Technologies)
</blockquote>
<blockquote>
Dr. Kyle Luttgeharm is a Product Manager with Agilent Technologies. He holds undergraduate degrees in Biology and Biochemistry from Southwestern College in Winfield, Kansas, and a doctorate in Biochemistry from the University of Nebraska-Lincoln. Dr. Luttgeharm previously worked as an Application Scientist with Advanced Analytical Technologies, Inc. and as an R&amp;D Scientist with Agilent Technologies. He has extensive experience developing methodologies and assays for Agilent’s parallel capillary electrophoresis technology.
</blockquote>
<blockquote>
Presenter: Dr. Gizem Bergdahl (Research Scientist, DAKO Denmark R&amp;D &amp; Manufacturing, Agilent Technologies, (Glostrup, Denmark)
</blockquote>
<blockquote>
Dr. Gizem Bergdahl has a deep understanding of diagnostics, clinical biochemistry, human diseases, and in vitro diagnostics, and extensive experience in areas including immunoassays (ELISA, immunoturbidimetry, and chemiluminescence), ligand-binding assays (SPR), antibody conjugation to latex, and magnetic beads.
</blockquote>
<blockquote>
Presenter: Blake Forman (Scientific Content Creator, SelectScience)
</blockquote>
<blockquote>
Blake studied chemistry at the University of Surrey, UK, and further went on to study a master’s in chemistry at the University of Southampton, UK. Blake has previously worked in the Medicines and Healthcare Products Regulatory Agency (MHRA) lab at LGC and as a technical sales specialist for Waters. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.
</blockquote>

Agilent: Improve laboratory efficiency with automated protein analysis

Improve laboratory efficiency with automated protein analysis

In this webinar, will be discussed how the ProteoAnalyzer system can transform protein analysis workflows.

Improve lab efficiency with automated protein analysis

Regardless of your sample, it is critical to simplify your routine analysis work and infuse as much automation as possible into your workflow so you can increase confidence in your results and reduce the time it takes to make decisions. Empower Software offers you the flexibility to optimize operations in your lab.
To ensure that your method is performing as expected, routine food analysis calls for calibration/quantitation along with system suitability and/or control samples. These applications call for some commonly made calculations such as:
<ul>
<li>Percent Difference compared to a Target Amount</li>
<li>Average Amount in Duplicate Samples</li>
<li>Percent Deviation for Standards</li>
</ul>
Determining authenticity of botanical ingredients used in dietary supplements can be challenging. This routine test is simplified by automating evaluation of the following:
<ul>
<li>Chromatographic Pattern</li>
<li>Mass Spectral Pattern</li>
<li>Spectral Library Match</li>
</ul>
To further streamline, using tailored reports in Empower Software makes interpretation of results easy and decision-making faster with simple pass/fail, so you know whether you can ship a batch.
Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.
<blockquote>
Presenter: Neil Lander (Principal Product Manager, Informatics, Waters Corporation)
</blockquote>
<blockquote>
Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.
</blockquote>

Waters Corporation: Empower Tips: Ask Me Anything About Streamlining Efficiencies in Your Routine Analysis – Food and Dietary Supplement Applications