What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Proper care and maintenance of HPLC columns are essential for getting accurate results and saving on replacement costs. Extending the HPLC column life not only ensures consistent performance but also reduces downtime and the need for frequent replacements.Routine maintenance is equally essential for sustaining column functionality. Correct handling of the column is paramount, ensuring strict compliance with the manufacturer’s specifications regarding pressure, pH, flow rate, and temperature.In the next section, we will explore these best practices in greater detail, offering further guidance on essential HPLC column maintenance strategies. Whether you’re working in a high-throughput lab, or conducting occasional analyses, these strategies will help you get the most out of your <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=48&amp;manufacturerItems=50">HPLC columns</a>.<h3>Essential Guidelines for Extending HPLC Column Life</h3>By adopting these simple yet effective practices, you can greatly extend the HPLC column life while ensuring reliable, high-quality results in your analyses.<h4>Proper Column Handling</h4>HPLC columns should be handled with care to prevent physical damage or shock. The pump should not be started at high flow rates; instead, the flow rate should be gradually increased over several minutes. A pump pressure limit should be set to protect the column from over pressuring, as sudden pressure changes can lead to voids, negatively affecting the column’s performance.<h4>HPLC Column Conditioning and Equilibration</h4>Conditioning is a critical step for preparing a new or stored HPLC column by replacing the storage solvent with the desired mobile phase. Care must be taken to avoid introducing immiscible solvent mixtures into the column. Universally miscible solvents such as THF (tetrahydrofuran) or IPA (isopropyl alcohol) can be useful for this process when moving from normal phase to reversed phase solvent systems. Care must also be taken to ensure that solvents that may be damaging to a specific stationary phase are avoided.For example, if a reversed-phase column experiences phase collapse due to 100% aqueous mobile phases, it should be reactivated. This process involves running 100% acetonitrile at a high flow rate and a temperature as high as 60°C. It is essential to ensure no solid buffers precipitate from residual solvents. Depending on the column condition, between 50 and 100 column volumes are needed for complete reactivation and equilibration, restoring the column’s efficiency for future use.<h4>Optimizing Operating Conditions</h4>Optimizing operating conditions is paramount to enhancing HPLC column efficiency and prolonging their operational lifespan. A fundamental aspect of this is strict adherence to the manufacturer’s recommended temperature, pH and pressure parameters.Deviating from these limits, particularly by operating at elevated temperatures, can significantly hasten the degradation of the stationary phase and hardware, thereby compromising overall performance. Elevated temperatures can also narrow the pH operating range, exacerbating the risk of column degradation through phenomena such as peak broadening or diminished efficiency.Effective pressure management is equally crucial. Sudden pressure surges or mechanical shocks can result in bed voiding, leading to poor chromatographic performance, such as split peaks and reduced separation efficiency. Gradual ramping of flow and pressure, implemented each time the column is initiated, mitigates these risks. Many modern HPLC systems are equipped with automated flow-pressure ramping capabilities, ensuring smoother transitions and reducing the potential for column damage.In addition, vigilant monitoring of the mobile phase's pH is vital to the maintenance of HPLC column integrity. Deviations from the optimal pH range can cause significant harm to the stationary phase. For instance, leads to ligand hydrolysis which leads to a loss of stationary phase functionality and diminished column performance over time. High pH can lead to silica degradation. This weakens the silica structure, causing particle dissolution, void formation, and loss of column efficiency. Maintaining the pH within optimal boundaries is essential for preserving the column’s structural integrity and ensuring consistent chromatographic performance.Furthermore, incorporating inline filters and guard columns into the system serves as a proactive measure to prevent particulates and harsh sample matrices from damaging the analytical column. These preventive tools significantly enhance operational conditions and safeguard the column from contamination, ultimately contributing to extended HPLC column lifetime and reliable performance.<h4>Regular Column Maintenance</h4>Effective routine maintenance is paramount to extending the HPLC column lifetime and ensuring the consistent performance of HPLC columns. A critical aspect of this maintenance is the thorough washing of the column after each use to eliminate residual sample components and mobile phase contaminants.The cleaning process generally involves performing an isocratic or gradient wash using a solvent system similar to the one previously used on the column but substituting the buffer with HPLC-grade water. This helps effectively remove any residual buffer components from the column. This meticulous cleaning process is vital to preventing the accumulation of impurities that could compromise the stationary phase and lead to decreased efficiency over time.Post-cleaning, it is imperative to end-cap the column before storage to prevent the stationary phase from drying out, a condition that can negatively impact the column’s future performance. Proper capping or sealing ensures that the column remains in optimal condition, safeguarding its structural integrity and readiness for subsequent analyses.Additionally, utilizing an older HPLC system as a dedicated wash station can further streamline maintenance practices, freeing up live systems for active use. Consistently adhering to a rigorous cleaning and storage regimen not only enhances the longevity of HPLC columns but also guarantees the delivery of precise and reliable analytical results.For severe contamination or pressure buildup, backflushing may be necessary to dislodge tightly bound contaminants. However, backflushing should only be performed when absolutely necessary, as it can stress the column packing material. It is essential to consult the manufacturer’s instructions before attempting this method.

How to Extend the Lifetime of HPLC Columns?

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Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Mettler-Toledo

Agilent Technologies

Bruker

GERSTEL

Thermo Fisher Scientific

SCIEX

Evosep Biosystems

From beginner to expert, we can all benefit from a review of HPLC basics. In this two-part talk, we will work our way from basic chromatographic principles to advanced method development topics.
Method Development 102 will review and expand upon some of the 101 fundamentals as we cover advanced topics such as how to best transfer a method from one column dimension to another. We will explore reasons for why some methods are quite difficult to transfer to a different HPLC system. In addition, we will look at gradient method development and how to efficiently use a scouting gradient to quickly develop a good HPLC method.
<blockquote>
Presenter: Paul Altiero (Application Engineer, Agilent Technologies, Inc.)
</blockquote>
<blockquote>
Paul Altiero is an Applications Engineer located at the Agilent Little Falls site in Wilmington, DE. Prior to coming to Agilent in 2009, Paul worked in the pharmaceutical industry performing LCMS bioanalysis supporting Drug Discovery DMPK. While at Agilent, Paul has been a field service engineer for LCMS as well as the integrating program manager for the RapidFire high throughput LCMS system. In his current role Paul’s responsibilities include technical support and application assistance for LCMS consumables.
</blockquote>

Agilent Technologies: HPLC Method Development: From Beginner to Expert, Part 2

HPLC Method Development: From Beginner to Expert, Part 2

Basic chromatographic principles to advanced method development topics.

PhenoAcademy Webinars
 Liquid Chromatography Series
<h4>Measuring Peak Separation Quality</h4>
<ul>
<li>Discover the methodologies for calculating resolution, asymmetry, selectivity, and efficiency</li>
<li>Learn how to quantify the quality of a separation, along with a reference of what each value could and should be</li>
<li>Understand the physicochemical underpinnings of chromatographic separations</li>
</ul>
<blockquote>
Presenter: Kevin Zhao (Senior Technical Specialist)
</blockquote>
<blockquote>
Kevin Zhao is the Senior Technical Specialist with Phenomenex and has 5 years of experience in biochemistry research; 4 years of Protein/Cancer research with CSULA-City of Hope Cancer Collaborative and over 9 years in Phenomenex. Mr. Zhao is a member of a global Technical Support Team which includes providing problem-solving, troubleshooting, and applications assistance for customers worldwide in the fields of chromatography (liquid and gas) and sample preparation, and also handling Industries supported include – pharmaceutical and biopharmaceutical, clinical research and clinical toxicology, food safety, environmental, fuels and specialty chemicals. Mr. Zhao received his B.S. in Biology at the California State Polytechnic University, Pomona. earned his M.S. in Biochemistry at California State University, Los Angeles, and also earned a MBA at the University of the Incarnate Word.
</blockquote>
<blockquote>
Presenter: Koen Askamp (Technical Specialist)
</blockquote>
<blockquote>
Koen is from the Netherlands, studied Molecular Life Sciences at Wageningen University (Netherlands), majoring in immunology. Analytical Chemistry was one of my favorite courses – who doesn’t love solving puzzles?
</blockquote>
<blockquote>
I like drumming, scuba diving, motorcycle-riding, and this past winter I learned how to ski (a little)!
</blockquote>
<blockquote>
Presenter: Namrata Saxena (Technical Manager)
</blockquote>
<blockquote>
Namrata Saxena is a Technical Manager - Asia/Pacific” with Phenomenex and is based in Sydney, Australia. She has a post graduate degree with a specialization in Analytical Chemistry, with extensive experience in the Liquid chromatography domain, and her current focus is in Omics and Biologics field. 
</blockquote>
<blockquote>
Before joining Phenomenex she had worked as “Product Specialist” and spent several years in column chemistry, column manufacturing and method development in the application and manufacturing labs of Bischoff Chromatography, Germany. 
</blockquote>
<blockquote>
In her current role, she provides product selection, method development, training and troubleshooting support to customers as well as Phenomenex Sales Consultants globally. 
</blockquote>

Phenomenex: PhenoAcademy Webinars - Liquid Chromatography Series

Measuring Peak Separation Quality

Discover the methodologies for calculating resolution, asymmetry, selectivity, and efficiency

Presentation Topics:
&quot;Measuring Volatile Short-chain Fatty Acids in Microbiome Samples,&quot; Dr. Justin Cross and Charlotte Pohl, Donald B. and Catherine C. Marron Cancer Metabolism Center, Memorial Sloan Kettering Cancer Center
Short-chain fatty acids (SCFAs) are produced through the fermentation of non-digestible carbohydrates by the intestinal microbiota. Besides their role in energy homeostasis, SCFAs have also been shown to have signaling roles in the host through the inhibition of histone deacetylases (HDACs) and the activation of G-protein-coupled receptors (GPCRs). We will describe a sensitive and robust gas chromatography-mass spectrometry (GC-MS) method to measure SCFAs using negative chemical ionization (nCI). The method includes automated off-line derivatization using pentafluorobenzyl bromide (PFBBr) and is performed on a GERSTEL MultiPurpose Sampler (MPS). We will highlight how the use of internal standards and calibration standard curves enables the precise quantitation of SCFAs and who GERSTEL automation solutions have significantly enhanced the throughput and reproducibility of our workflow. 
&quot;Automated Hybrid Analytical Workflows for Human Health and Nutrition Research: Lipids and Lipophilic Hormones,,&quot; Dr. Michael Bukowski, USDA-ARS-Beltsville Human Nutrition Research Center
The analysis of lipids and lipophilic small molecules with conventional pipette-based robotics systems is challenging due to the aggressive organic solvents needed extract and solubilize these species. In this presentation, the application of an automated workstation to hybrid workflows for lipids and stress hormones will be demonstrated. The first workflow in support of an epigenetics program stemmed from limited samples of placental tissue. The second workflow presented was developed in support of a project examining chronic stress response as exhibited in hair cortisol.

GERSTEL: Automated, Robust, and Reproducible Sample Preparation Protocols in the Field of Omics

Automated, Robust, and Reproducible Sample Preparation Protocols in the Field of Omics

Measuring Volatile Short-chain Fatty Acids in Microbiome Samples and Automated Hybrid Analytical Workflows for Human Health and Nutrition Research

Automated Sample Preparation Protocols in the Field of Omics

The adoption of high-throughput techniques in plasma proteomics has transformed the discipline by enabling the simultaneous analysis of large patient cohorts and the identification of hundreds to thousands of proteins.
This strategy has facilitated the uncovering of new biomarkers and therapeutic targets that had not been identified before.
Learn more about scalable plasma proteomics workflows featuring the Evosep One in this webinar.
<h4>Metasight Diagnostics</h4>
<ul>
<li>TALK BY BORIS SARVIN, VP TECHNOLOGY, METASIGHT DIAGNOSTICS</li>
</ul>
Our seminar introduces Metasight Diagnostics. We developed a platform powered by Evosep technology that utilizes a fully automated robotic protocol for sample preparation and LCMS-based proteomics. Analyzing thousands of serum samples, this approach pioneers the development of signatures for early diagnostics across various health conditions. With access to anonymized health records data and a unique serum sample collection, our pipeline aims to revolutionize early disease detection, offering valuable insights and opportunities for proactive healthcare intervention. Join us as we explore the innovative advancements shaping the future of early diagnostics
<h4>Evotip Pure enabled workflows for standardized and scalable plasma profiling</h4>
<ul>
<li>TALK BY STEPHANIE SAMRA, SENIOR MANAGER, TRANSLATIONAL RESEARCH MARKETING, THERMO FISHER SCIENTIFIC</li>
</ul>
Plasma is an important biofluid used for clinical diagnosis, readily obtainable and carrying a wide range of organ or tissue secreted biomolecules that could be used as reporters of a patient health status. To effectively transition from biomarker discovery to verification and ultimately clinical application there is a need for robust, high throughput and cost-effective plasma profiling workflows. We describe a fully automated and modular pipeline for neat as well as deep plasma proteome analysis. The hands-off methods integrate all steps from raw plasma to peptides loaded on Evotips and can robustly process up to 192 samples. At a throughput of 100SPD approximately 1,500 and 5,000 protein groups could be quantified using the neat and deep plasma profiling workflows. Low abundance, clinically relevant FDA and putative cancer biomarkers could be readily detected.

Evosep Biosystems: SCALABLE PLASMA PROTEOMICS WITH THE EVOSEP ONE

SCALABLE PLASMA PROTEOMICS WITH THE EVOSEP ONE

Explore how the Evosep One, with high reproducibility and robustness, is ideally suited for analysis of large sample cohorts of blood plasma.

In recent years, the use of quantitative liquid chromatography (LC) coupled charged aerosol detection (CAD) for poor UV absorbing analytes in multicomponent mixtures has grown exponentially across industrial sectors.
In this live webinar, we will highlight some distinctions between CAD and other UV-absorbance detection, such as the non-linear response to analyte concentration, and sensitivity to mobile phase impurities. However, careful optimization of CAD parameters can lead to successful development and transfer of analytical methods, nearly on par with that of conventional UV absorbance detectors. Through careful tuning CAD parameters, we have developed quantitative LC-CAD assays which are sensitive, precise, accurate and robust across early and late-stage pharmaceutical development. Examples of LC-CAD methods across various stages of drug development spanning from discovery to commercialization will be shared during the presentation. 
Learning points:
<ul>
<li>Optimization: Optimizing non-linear detection response</li>
<li>Troubleshooting: Tips and tricks to maintain a CAD detector</li>
<li>Transfer: Examples of HPLC-CAD successfully developed and transferred (nearly on par with conventional HPLC-UV) </li>
<li>Implementation of HPLC-MS-CAD for post µg-purification analysis</li>
</ul>
Who should attend:
<ul>
<li>Analytical chemists: Professionals who specialize in analytical techniques, especially those involved in liquid chromatography and detection methods</li>
<li>Pharmaceutical scientists: Researchers and scientists working in the pharmaceutical industry, particularly those involved in drug development and quality control </li>
<li>Quality control and assurance professionals: Individuals responsible for ensuring the quality and reliability of analytical methods used in various industries, including pharmaceuticals</li>
</ul>
<blockquote>
Presenter: Imad Haidar Ahmad, PhD (Research Director, Amgen)
</blockquote>
<blockquote>
Presenter: Wes Barnhart (Principal Scientist, Amgen)
</blockquote>

ThermoFisher Scientific: Back to basics: Revealing the strength of HPLC-CAD methods

Back to basics: Revealing the strength of HPLC-CAD methods

This webinar will highlight some distinctions between CAD and other UV-absorbance detection

Revealing the strength of HPLC-CAD methods

Oligonucleotide characterization by mass spectrometry has gained significant interest with the increased use of DNA and RNA as research reagents and pharmaceutical drug molecules. Characterization of production related impurities or degradation products is of key importance during the approval process of such biopharmaceutical products. 
In recent years, LC-MS based methods have become well-established tools for this characterization process. During this &quot;Live from the Lab&quot; session we will show from within the Bruker Demonstrations Laboratory in Bremen, how the Bruker timsTOF mass spectrometer facilitates the characterization of oligonucleotides and oligonucleotide degradation products. We will show how easy related side products can be found, characterized, and quantified automatically with the OligoQuest™ workflow within the BioPharma Compass® Software.
We welcome you to this Live from the lab session and look forward to an interesting discussion.
Who should attend:
<ul>
<li>Scientists interested in Oligonucleotide characterization</li>
<li>Oligonucleotides QC, RNA and DNA drug development</li>
</ul>
<blockquote>
Presenter: Eric Van Beelen, Ph.D., Business Development Manager Pharmaceuticals Market, Bruker Daltonics
</blockquote>
<blockquote>
Presenter: Detlev Suckau, Ph.D., Pharma/Biopharma Applications Software, Bruker Daltonics
</blockquote>
<blockquote>
Presenter: Oskar Knittelfelder, Ph.D., Field Application Scientist, Pharma/Biopharma Applications, Bruker Daltonics
</blockquote>

Bruker: Live from the lab: Oligonucleotide characterization workflows with mass spectrometry

Live from the lab: Oligonucleotide characterization workflows with mass spectrometry

We will show how the Bruker timsTOF mass spectrometer facilitates the characterization of oligonucleotides.

Oligonucleotide characterization with mass spectrometry

The MALDI HiPLEX-IHC workflow, with photocleaveable mass tags from AmberGen, offers the unique capability to evaluate spatial information of a targeted panel of proteins within a cancer sample.
Tune in to hear about how this workflow has added value to the research being done by Dr. Peggi Angel, which will be followed up by a thorough demonstration of the HiPLEX-IHC workflow from our application scientists! They will walk you through the basics of MALDI Imaging and how to prepare samples successfully for targeted protein experiments in order to take your cancer biology research to the next level.
Key learning objectives:
<ul>
<li>Overview of the study of cancer through MALDI Imaging, specifically utilizing MALDI HiPLEX-IHC </li>
<li>Introduction to sample preparation for this workflow, with a highlighted focus on the overall simplicity</li>
<li>Understanding of the value of integrating MALDI-HiPLEX IHC with other MALDI Imaging and non-MALDI Imaging approaches to provide greater biological context</li>
</ul>
Who should attend:
<ul>
<li>Research scientists with limited or no exposure to MALDI Imaging who are interested in cancer biology</li>
<li>Research scientists with active MALDI Imaging experience but limited exposure to MALDI HiPLEX-IHC</li>
</ul>
<blockquote>
Presenter: Peggi Angel, Ph.D. (Assistant Professor, Medical University of South Carolina, Charleston, SC, USA)
</blockquote>
<blockquote>
Dr. Peggi Angel is an Assistant Professor at the Medical University of South Carolina, where she has developed numerous technological advances of MALDI Imaging for application to the study of cancer and cardiovascular disease. Dr. Angel currently serves as the President of the Imaging Mass Spectrometry Society (IMSS). She has led the way in MALDI Imaging for numerous application areas, including high spatial resolution imaging, cell culture imaging, and targeted protein imaging. Dr. Angel has significantly utilized the MALDI HiPLEX-IHC workflow as an important tool in her research work, including for multiple high-impact publications. Dr. Angel’s current work focuses on high-throughput analysis of extracellular matrix and N-glycans in cancer and cardiovascular disease.
</blockquote>
<blockquote>
Presenter: Andrew DelaCourt, Ph.D. (Field Application Scientist in Imaging, Bruker, Charleston, SC, USA)
</blockquote>
<blockquote>
Dr. Andrew DelaCourt is a Field Application Scientist at Bruker focusing on MALDI Imaging applications. Dr. DelaCourt recently completed his Ph.D. work in the laboratory of Dr. Anand Mehta at the Medical University of South Carolina, where he utilized MALDI Imaging for the identification of N-linked glycan biomarkers of genetic subtypes of hepatocellular carcinoma. Dr. DelaCourt has authored multiple research articles on the topics of hepatocellular carcinoma, glycobiology, and the development of MALDI Imaging workflows to target specific N-glycans of interest. Dr. DelaCourt brings his expertise in cancer biology and glycobiology to Bruker in order to further contribute to the application of MALDI Imaging in those areas.
</blockquote>
<blockquote>
Presenter: Daniel Castro, Ph.D. (Application Scientist in Imaging, Bruker, Billerica, MA, USA)
</blockquote>
<blockquote>
Dr. Daniel Castro is an Applications Scientist at Bruker focusing on MALDI imaging. Dr. Castro completed his Ph.D. in the lab of Jonathan Sweedler at the University of Illinois-Urbana-Champaign, where he focused on high-throughput single-cell and single-organelle measurements as well as accelerating tissue MALDI-FTICR imaging. Dr. Castro brings his expertise in high-throughput MALDI measurements to Bruker.
</blockquote>
<blockquote>
Presenter: Catherine Kita (Scientist, AmberGen, Billerica, MA, USA)
</blockquote>
<blockquote>
Catherine Kita is a Scientist at AmberGen focusing on MALDI-IHC. Catherine completed her Master’s at Drexel University, where she focused on Sequential Elution LC-MS method development utilizing a pH gradient. Prior to joining the AmberGen team, Catherine worked developing LC, LC/MS and biological assays for the pharmaceutical sector. Catherine is currently working on the development of targeted MALDI-IHC panels for a variety of cancers and neurological diseases.
</blockquote>

Bruker: LabCast: Targeted Analysis of Proteins in Breast Cancer using the MALDI HiPLEX-IHC workflow

LabCast: Targeted Analysis of Proteins in Breast Cancer using the MALDI HiPLEX-IHC workflow

Demonstration of the MALDI HiPLEX-IHC workflow added value to the research of proteins within a cancer sample.

Analysis of Proteins in Cancer using the MALDI HiPLEX-IHC

<h3>LC-MS/MS 201 webinar series</h3>
Advanced techniques for data acquisition and processing
<h4>Elevate your analytical expertise with advanced techniques and workflows</h4>
Sharpen your data acquisition and processing skills with our LC-MS/MS 201 webinar series. Based on our LC-MS/MS 101 webinar series, these sessions will go beyond the fundamentals of LC-MS/MS and introduce ways to advance your analytical methods and data processing workflows. Every other week, we will explore how to optimize SCIEX OS software with short, 30-minute expert-led presentations.
<h3>This webinar:</h3>
<h4>April 4, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - It&#39;s a TRAP! Getting the most out of your QTRAP system</h5>
<ul>
<li>Learn when to utilize QTRAP functionality to create more selective and sensitive methods.</li>
</ul>
<h3>Other webinars from the series:</h3>
<h4>March 21, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3705">Mastering chromatography</a></h5>
<ul>
<li>Discover how to optimize your chromatography for even the most challenging compounds while creating and building LC methods.</li>
</ul>
<h4>April 18, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3707">Troubleshooting at the source</a></h5>
<ul>
<li>Acquire the knowledge needed to optimize source parameters and identify and resolve ionization-related problems.</li>
</ul>
<h4>May 2, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3708">Automating data processing</a></h5>
<ul>
<li>Learn how to automate your data processing with custom flagging and rules for efficient data review in SCIEX OS software.</li>
</ul>
<h4>May 16, 2024</h4>
<h5>10:00 a.m. PT/ 1:00 p.m. ET - <a href="https://lcms.labrulez.com/webinar/3709">Excel strategies for mass spectrometry data analysis</a></h5>
<ul>
<li>Take an in-depth look at when and how to use Excel to increase your productivity.</li>
</ul>

It's a TRAP! Getting the most out of your QTRAP system

Learn when to utilize QTRAP functionality to create more selective and sensitive methods.

The speed and quality of fluorescence-based high-throughput screening (HTS) methods have become critical problems in the drug discovery process, yet these methods remain common due to their sensitivity, ease of use and adaptability to HTS formats. The use of labeled compounds increases the cost per sample and the detected response can be affected by fluorescent quenchers, colored compounds and form aggregates. These pan-assay interference compound (PAIN) effects are problematic, as they produce false positive results and are difficult to predict.
Alternative approaches to fluorescence assays for high-throughput sample analysis are being implemented, such as mass spectrometry, which utilizes label-free detection of the analyte using the mass-to-charge ratio. However, the traditional LC-MS analysis of 384 samples requires hours, compared to the seconds needed fora plate reader, and typically necessitates different buffer requirements. The Echo® MS+ system with the ZenoTOF 7600 system presents an alternative approach for analyzing small molecules and intact proteins and can solve some of the challenges faced by traditional techniques in biologically relevant buffers.
In this webinar you will learn:
<ul>
<li>AEMS technology</li>
<li>Key features of new Echo® MS+ system</li>
<li>HT applications (intact proteins)</li>
</ul>
<blockquote>
Presenter: Jacob W. McCabe, Ph.D. (Senior Scientist – Echo MS Excellence Team, SCIEX)
</blockquote>
<blockquote>
Jacob W. McCabe, Ph.D. is a Senior Scientist at SCIEX in the Echo® MS Center of Excellence in Framingham, MA. The lab is a dynamic and interactive scientific showroom containing new and cutting-edge innovations in mass spectrometry. Jacob and colleagues collaborate with researchers from around the globe to develop new approaches to high throughput quantitative mass spectrometry across a range of molecule types and analytical challenges.He holds a Bachelor of Science degree in chemistry from Baylor University, a Master of Science from Texas A&amp;M University – Commerce, and a Doctor of Philosophy in Analytical Chemistry from Texas A&amp;M University studying under Professor David H. Russell. 
</blockquote>

SCIEX: Advancing Acoustic Ejection Mass Spectrometry: next-generation platform for high-throughput sample analysis

Advancing Acoustic Ejection Mass Spectrometry: next-generation platform for high-throughput sample analysis

The Echo® MS+ system with the ZenoTOF 7600 system presents an alternative approach for analyzing small molecules and intact proteins.

Acoustic Ejection Mass Spectrometry for HTS analysis

<h5>Event Program</h5>
<ul>
<li>What exactly is data integrity for electronic record keeping and how does it affect process instrumentation for pharmaceutical water systems?</li>
<li>How can a transmitter and software combination help pharmaceutical companies implement data integrity?</li>
</ul>
<h5>Easy Data Integrity Implementation</h5>
In this live webinar, we will discuss what data integrity for electronic record keeping is and how it impacts process instrumentation for pharmaceutical water systems. 
The presenter will also introduce updates and additions to the RecordLOC transmitter and PC software, the METTLER TOLEDO Thornton solution for implementing data integrity for TOC, ozone, and conductivity in pharmaceutical water systems. 
See how RecordLOC helps pharmaceutical companies easily implement data integrity.
Attendees will have the opportunity to ask the presenter your questions about electronic record keeping.
<blockquote>
Presenter: Joel Kenyon (Senior Global Product Manager for Conductivity and Transmitters for METTLER TOLEDO Thornton.)
</blockquote>
<blockquote>
Joel Kenyon is the Senior Global Product Manager for Conductivity and Transmitters for METTLER TOLEDO Thornton. A biologist by training, for the last 15 years Joel has developed and supported analytical instrumentation for academic and industrial applications across a broad range of disciplines.
</blockquote>

Mettler-Toledo: Data Integrity Requirements For Process Instrumentation

Data Integrity Requirements For Process Instrumentation

We will discuss what data integrity for electronic record keeping is and how it impacts process instrumentation for pharmaceutical water systems.