Oligonucleotide and mRNA Analysis Resource Roundup

What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Article

Application

Popularization

Science and research

Poster

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Agilent Technologies

Technology Networks

Bruker

SCIEX

Waters Corporation

<h4>Virtual event: Pharma conclave series 2024</h4>
SCIEX offers the platform for the experts to take the stage and drive technological progress
Welcome to Pharma conclave series 2024 – where our customers’ expertise meets innovation, transforming your knowledge and empowering you to revolutionize your work processes. Join us in discovering techniques that will enhance your skills to accelerate your daily operations in the development of new drugs.
From the research of new markers to the drug development moving into non and GLP bioanalysis. Confidently plan your next lab expansion with the knowledge gained from industry leaders and pioneers.
<ul>
<li>May 16 Event 1: Pharma omics</li>
<li>September 17 Event 2: Impurities</li>
<li>October 23-24 Event 3: Pharma</li>
</ul>
10:00 a.m.–12:00 p.m. CET
<h3>Event 2: Impurities</h3>
Impurities in the pharmaceutical industry. What&#39;s next?
While the regulatory landscape is changing faster than ever, having the right information on impurities available to you at every stage of the drug development is vital to bring safe and effective therapies to the market. Whether you want to optimize your lab’s current capabilities or scale up to meet your future demands, this event creates a platform to share the customer’s expertise.
<blockquote>
Presenter: Bruno Manadas, PhD
</blockquote>
<blockquote>
Dr Manadas is a prominent Cell Biology researcher at CNC-UC since 2017. With expertise in biochemistry, psychology, and biotechnology, he&#39;s authored 130+ publications and led multiple projects. As head of CNC&#39;s Mass Spectrometry node and Associate Editor for Frontiers, he&#39;s contributed significantly to neuroscience and psychiatry. Bruno&#39;s research focuses on cell biology mechanisms, translational biomarker research, and MS-based approaches for studying protein oxidation. He played a pivotal role in establishing the hospital biobank and teaches at the Portuguese Catholic University.
</blockquote>
<blockquote>
Presenter: Dorte Bekker-Jensen
</blockquote>
<blockquote>
Dorte is heading the Applications Development team in Proteomics Research at Evosep. She has long-term experience within proteomics and has focused on technology development by developing simple and automated workflows for high-throughput applications.
</blockquote>
<blockquote>
Presenter: Stefan Ledinger
</blockquote>
<blockquote>
Stefan has 20 years of experience in the life sciences and diagnostic industries. For the last dozen years, he has been serving various functions within biocrates life sciences ag. Most recently, he has been responsible for the market development for metabolomics solutions in the pharmaceutical and biotech industries. He currently serves as Global Head of Sales. Stefan holds degrees in business administration and bioengineering, combining commercial know-how with a passion for science.
</blockquote>
<blockquote>
Presenter: Markus Langsdorf
</blockquote>
<blockquote>
Markus has 14 years of experience in life sciences industries. As he joined biocrates in 2010, he worked for 11 years as application support specialist for biocrates’ metabolomics profiling solutions. 3 years ago he changed his position withing the company to his new function as product manager. Markus studied chemistry and graduated with a PhD in Analytical Chemistry.
</blockquote>

Pharma conclave series 2024: Impurities

As part of the global effort to reduce CO₂ emissions, biofuels are becoming an increasingly important source of green energy.
Quality control testing of biofuels is therefore crucial to ensure the efficiency and reliability of biogas, biodiesel, and fuel ethanol. 
In this webinar you will learn about the latest laboratory testing techniques that are used to ensure the quality, efficiency, and sustainability of modern biofuels to enable producers to confidently meet international regulatory requirements and to promote their widespread adoption in both industry and the wider society at large. 
Experts from Agilent Technologies will introduce a wide range of analytical testing solutions covering multiple technologies that are routinely used to detect organic and inorganic impurities in biodiesel and enable the stringent quality control of fuel ethanol, biogas and biogas blends to be achieved. The expert panel will be available to answer questions from the audience at the end of the presentation. 
<blockquote>
Presenter: Danny van Oevelen (LC Product Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Luca Godina (GC &amp; GC/MS Application Specialist, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Sebastien Sannac (Spécialiste produit spectroscopie atomique, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Donald Inglis (Product Specialist EMEAI for FTIR, Agilent Technologies)
</blockquote>
<blockquote>
Presenter: Remko van Loon (GC &amp; GC/MS Product Specialist, Agilent Technologies)
</blockquote>

Agilent Technologies: Comprehensive Quality Control Testing of Biofuels to Ensure a Greener, more Sustainable Future

Comprehensive Quality Control Testing of Biofuels to Ensure a Greener, more Sustainable Future

Learn about the latest laboratory testing techniques that are used to ensure the quality, efficiency, and sustainability of modern biofuels.

Comprehensive Quality Control Testing of Biofuels

Capillary electrophoresis (CE) is transforming viral vector manufacturing for next-generation biopharmaceuticals. Traditionally used for quality control, CE can now be used to profile viral proteins in AAV particles and examine genome integrity during production, thanks to novel sample preparation techniques.
In this webinar, our expert speakers will explore the different ways that CE is advancing biopharmaceutical development. They will also discuss how new reference standards are improving our understanding of genome and protein sizes in viral particles and how this impacts the development of viral vector-based therapies.
Attend this webinar to:
<ul>
<li>See how CE is being utilized to profile viral proteins in AAV particles during manufacturing</li>
<li>Discover how CE is instrumental in examining the genome integrity of viral particles throughout production</li>
<li>Explore how the adoption of new reference standards is enhancing the understanding of genome and protein sizes during viral particle manufacturing</li>
</ul>
<blockquote>
Presenter: Yolande Masson (Analytical Scientist at Yposkesi, an SK Pharmteco Company)
</blockquote>
<blockquote>
After a Master&#39;s degree in Cell and Gene Biotherapies, Yolande began her professional career as cell therapy engineer, focusing on the development of skin cell production protocols for clinical trials and associated controls. She joined the Centre d&#39;eXpertise Analytique (CXA) team in 2019, specializing in the development, validation and transfer of control methods for viral vectors gene therapy. She developed expertise in cell-based assay, flow cytometry and AUC. For the past year, she has been collaborating with SCIEX on implementing analytical methods for the control of rAAVs genome integrity using capillary electrophoresis.
</blockquote>
<blockquote>
Presenter: Dr. Stephen Lock (Senior Market Development Manager, Biopharma at SCIEX)
</blockquote>
<blockquote>
Dr. Stephen Lock obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. Previously a lecturer of Chemistry at the University of Hull he has worked for SCIEX in various technical roles for over 25 years. He has over 30 years’ experience in Analytical Chemistry and has presented at nearly one hundred international meetings around the world. Steve is currently the EMEAI Biopharma Senior Market Development Manager for SCIEX and is also an Adjunct Professor at the University of Pannonia where he acts in a volunteer role as a Scientific Advisor for the Horváth Csaba Memorial Laboratory lead by Prof. Andras Guttman. At the Horváth Csaba Memorial Laboratory Steve provides technical advice and supports the groups fundamental research and development of new applications for capillary electrophoresis.
</blockquote>
<blockquote>
Presenter: Juliette Laigle (Analytical Scientist at Yposkesi, an SK Pharmteco Company)
</blockquote>
<blockquote>
After obtaining her Master&#39;s degree in Pharmaceutical Biotechnology and Advanced Therapies, Juliette began her professional career as a Quality Control Engineer at Yposkesi, focusing on the validation of analytical methods used for viral vectors control. She then joined the Analytical eXpertise Center (CXA) team at its inception, specializing in the development, validation, and transfer of these methods, while also working on the implementation and automation of various analytical techniques. For the past year, she has been collaborating with SCIEX on implementing analytical methods for the control of rAAVs using capillary electrophoresis.
</blockquote>

Technology Networks: Transforming Viral Vector Manufacturing With Capillary Electrophoresis

Transforming Viral Vector Manufacturing With Capillary Electrophoresis

In this webinar, our expert speakers will explore the different ways that CE is advancing biopharmaceutical development. 

Viral Vector Manufacturing With Capillary Electrophoresis

<h4>Live from the Lab hosted by NIBRT: Solutions for biotherapeutic analysis</h4>
By your side to accelerate your biopharmaceutical development journey
Join us for the second Live from the Lab of 2024, taking place on September 17th, an exciting biopharma hybrid event featuring the latest advancements in separation technology and mass spectrometry. Focusing on all stages of biotherapeutics development, from discovery and research, all the way to routine and QC analysis, this event will give you valuable insights to accelerate your biopharmaceutical development journey.
The following speakers will talk at this event at the NIBRT research facility which will be streamed live all over Europe:
<ul>
<li>Dr. Jonathan Bones, NIBRT</li>
<li>Prof. Mark Dickman, University of Sheffield</li>
<li>Dr. Dan Bach Kristensen, Symphogen</li>
<li>Dr. Mark Hilliard, Pfizer</li>
<li>Dr. Ken Cook, Thermo Fisher Scientific</li>
<li>Jon Bardsley, Thermo Fisher Scientific</li>
</ul>
Whether you are a researcher, scientist, or industry professional in our region, this biopharma event is not to be missed. Expand your knowledge, network with experts, and discover how the Thermo Scientific solutions will stand by your side to accelerate your biopharmaceutical development journey.
Mark your calendars for September 17th and join us for this impactful hybrid event foster collaboration within the scientific community.
Participation is free of charge, registration is required.
For any questions about this event or regarding your registration, please email CMDEvents@thermofisher.com.
<h4>How to attend?</h4>
3 ways of attending:
<h5>Online attendees (remote):</h5>
Unable to travel to a satellite? Not a problem! You can register to attend conveniently online from your desk or anywhere else through the live stream from NIBRT.
<ul>
<li><a href="https://assets.thermofisher.com/TFS-Assets/CMD/Flyers/fl-003119-pb-live-from-the-lab-ms-biotherapeutics-agenda-online-fl003119-em-en.pdf">Agenda</a></li>
</ul>
<h5>NIBRT Research Facility (in-person event):</h5>
Join the heart of the event where the main agenda will be hosted and live streamed across our region to our satellite events and our online audience. Attendance through VIP invitation only!
<h5>Join a ‘Satellite’ location (in-person event):</h5>
Across Europe, our in-person satellite events will be broadcasting the main agenda live streamed from NIBRT, Dublin and supplement the day with local speakers/lab tours pending on the satellite. Take this opportunity to network with fellow scientists and meet your Thermo Fisher representatives from technical, commercial, and service functions. Check out the closest location to you and their individual agenda!
<ul>
<li>Søborg, Denmark (AGC Biologics)</li>
<li>Dreieich, Germany (Thermo Fisher Scientific)</li>
<li>Segrate, Italy (MI) (Thermo Fisher Scientific S.P.A.)</li>
<li>Leiden, Netherlands (Janssen Biologics BV)</li>
<li>Ljubljana, Slovenia (Four Points by Sheraton Ljubljana Mons)</li>
<li>Reinach, Switzerland (Thermo Fisher Scientific)</li>
<li>Hemel Hempstead, UK (Thermo Fisher Scientific)</li>
</ul>

Thermo Fisher Scientific: Live from the Lab: Solutions for biotherapeutics

Live from the Lab: Solutions for biotherapeutics

An exciting biopharma hybrid event featuring the latest advancements in separation technology and mass spectrometry.

From developability assessment to commercial manufacturing, the development of biopharmaceutical drug products entails multiple characterization challenges, made all the more complex when considering the range of diverse molecular formats from proteins to advanced therapy medicinal products (ATMPs).
Light scattering technologies provide key characterization tools that are key to addressing many of these challenges. Chief among these are multi-angle light scattering coupled to size-exclusion chromatography (SEC-MALS) and dynamic light scattering (DLS) performed without separation. Both techniques determine essential biophysical attributes in an absolute manner, rapidly and with no need for calibration against reference material or laborious sample preparation.
Following an introduction to light scattering technology and applications, this webinar will cover three case studies in the uses of SEC-MALS and DLS to characterize different types of biopharmaceuticals: antibodies, pegylated enzyme therapeutics and polyA-LNP. Attributes measured range from size and polydispersity, molar mass distribution and PEG-protein ratio to solution viscosity. These studies showcase the effectiveness of light scattering in supporting biopharmaceutical development towards successful commercialization.
Key Learning Objectives:
<ul>
<li>Strategies for assessing developability and optimizing drug formulations.
</li>
<li>Technology and applications of light scattering for biopharmaceutical characterization.
</li>
<li>Application of advanced analytical techniques (DLS, SEC-MALS) for biopharmaceutical characterization.
</li>
<li>Addressing challenges specific to different biopharmaceutical molecule formats.
</li>
</ul>
<blockquote>
Presenter: Andrei Hutanu, Ph.D. (ten23 health)
</blockquote>
<blockquote>
Andrei Hutanu attended the Technical University of Munich from 2012-2018 where he got his degree in Biochemistry. He then started his Ph.D. at F. Hoffmann - La Roche Ltd with research focus on enhancing capillary electrophoresis (CE) methods for biopharmaceuticals. During his Ph.D., Andrei has established analytical methods for new modalities like mAb co-formulations and bispecific mAbs. In 2019, he switched his focus to method development for cell and gene therapy products with a special focus on recombinant adeno associated viruses. Since 2023, he is part of the analytical development team of ten23, a CDMO that aims to develop, manufacture, and test tomorrow’s medicines.
</blockquote>
<blockquote>
Presenter: Michal M. Raczy, Ph.D. (ten23 health)
</blockquote>
<blockquote>
Dr. Michal M. Raczy is a Senior Scientist for Drug Product Design at ten23 health. Since joining the company in January 2022, he has led formulation and process development projects, while also driving innovation within biologics and surfactants analytics. With a strong background in chemistry and nanotechnology, Dr. Raczy obtained his PhD in molecular engineering from the University of Chicago (USA). During his doctoral studies, he conducted research on novel drug delivery systems and immunomodulatory biomaterials, leading to several published papers in prestigious peer-reviewed journals.
</blockquote>
<blockquote>
Presenter: Daniel Some, Ph.D. (Waters | Wyatt Technology)
</blockquote>
<blockquote>
Dan Some leads product marketing for Wyatt’s process analytical and macromolecular interaction instrumentation. Prior to joining Wyatt he was involved in semiconductor and military electro-optics industries. Dan obtained his Ph.D in Physics from Brown University and carried out postdoctoral research at Los Alamos National Lab and Weizmann Institute of Science.
</blockquote>

Waters Corporation: Developing Diverse Biopharmaceuticals with Light Scattering

Developing Diverse Biopharmaceuticals with Light Scattering

Strategies for assessing developability and optimizing drug formulations.

Fragmentation-based molecular annotations are the gold standard in high-confidence biomarker identification using mass spectrometry.
The new iprm-PASEF workflow integrates CCS-enabled MALDI Imaging of lipids with a targeted MS/MS imaging approach that allows spatial fragmentation and analysis of multiple targets in a single acquisition. The workflow includes a fully integrated pipeline within our software ecosystem to generate accurate identifications with spatial fidelity.
Key Learning Objectives:
<ul>
<li>Fragmentation based MALDI Imaging capabilities
</li>
<li>iprm-PASEF workflow for the timsTOF fleX
</li>
<li>Fragmentation based annotation pipeline using SCiLS™ Lab
</li>
</ul>
Who should attend:
<ul>
<li>Researchers interested in expanding their spatial biology toolkit
</li>
<li>Researchers focused on maximizing confidence of molecular annotations for MALDI Imaging
</li>
<li>Anyone who wants to expand their MALDI Imaging workflows
</li>
</ul>
<blockquote>
Presenter: Michael Becker, Ph.D. (Principal Scientist DMPK, Boehringer Ingelheim Pharma GmbH &amp; Co KG, Mainz, Germany)
</blockquote>
<blockquote>
Dr. Michael Becker is the head of the Mass Spectrometry Imaging lab at Boehringer Ingelheim. He received his Ph.D. in Neurobiology, and transitioned into the imaging field more than 15 years ago. He uses MSI to study drugs and drug-induced changes in tissue. To do so, he tries to push the sensitivity, reproducibility and specificity of the method.
</blockquote>
<blockquote>
Presenter: Arne Behrens, Ph.D. (Application Specialist in MALDI Imaging, Bruker Daltonics GmbH &amp; Co. KG, Bremen, Germany)
</blockquote>
<blockquote>
Dr. Arne Behrens is an Imaging Applications Scientist  at Bruker in Bremen with more than 6 years of experience on the timsTOF fleX platform. He received his Ph.D. in Analytical Chemistry and is an expert for CCS-enabled MALDI Imaging applications.
</blockquote>
<blockquote>
Presenter: Björn Wendik, Ph.D. (Business Development Manager, Bruker Daltonics GmbH &amp; Co. KG, Bremen, Germany)
</blockquote>
<blockquote>
Björn studied biology at the University of Würzburg and University of Freiburg with focus on developmental biology. In Freiburg he did his Ph.D. working on motoneuron development and pancreas formation. He became an expert on life cell imaging, timelapse studies and 3d-image software analyses. He continued his work as lecturer at the University of Freiburg where he also took care of parts of the Imaging core facility. When stepping into industry, he worked as a specialist for confocal microscopy and from there moved on into multispectral imaging, antibody based multi- and hiplexing and tissue analysis platforms. With this expertise, Björn started as a Business Development Manager for the MALDI Imaging at Bruker in July 2023.
</blockquote>

Bruker: Confidence in spatial identifications using iprm-PASEF on the timsTOF fleX

Confidence in spatial identifications using iprm-PASEF on the timsTOF fleX

The new iprm-PASEF workflow integrates CCS-enabled MALDI Imaging of lipids with a targeted MS/MS imaging approach.

Confidence in spatial identifications using iprm-PASEF

<h3>Session #2 - Innovative Approaches in HRMS and Software for PFAS Analysis</h3>We are excited to invite you to our upcoming webinar, where we will explore the latest advancements in PFAS analysis through innovative screening methods and technologies. Our expert speakers will cover various approaches to enhance the detection and identification of per- and polyfluoroalkyl substances (PFAS).<h4>1. Nontarget Screening workflow (NTS) for the Analysis of Per and Polyfluoroalkyl Substances (PFAS) using High resolution mass spectrometry (HRMS)</h4><ul><li>Presented by Hania Khoury-Hollins</li></ul>This session will focus on using HRMS as an effective tool to detect both regulated and non-regulated PFAS. We’ll explore the UNIFI™ screening workflow and discuss the use of comprehensive PFAS libraries to improve detection capabilities.<h4>2. Untargeted Screening for PFAS Chemicals Using Comprehensive Feature Detection</h4><ul><li>Presented by Jeff Goshawk</li></ul>Discover how advanced feature detection methods can identify a wide range of PFAS chemicals, including those not covered by traditional reference standards. This talk will highlight techniques to detect novel PFAS-like chemicals, providing a broader understanding of total organic fluorine content in samples.<h4>3. Elevating Specificity and Identification Confidence for PFAS Liquid Chromatography and Cyclic Ion Mobility Mass Spectrometry</h4><ul><li>Presented by Michael McCullagh</li></ul>Learn how cyclic ion mobility (cIM) enhances the separation and identification of PFAS isomers. By using this method, we can achieve greater specificity and reduce analysis time, providing insights into PFAS exposure and its potential health impacts.Join us to learn how these cutting-edge approaches are revolutionizing PFAS detection and analysis, offering more accurate and efficient solutions for environmental monitoring and public health protection.<blockquote>Presenter: Hania Khoury-Hollins (Principal Scientist, Waters Corporation)Hania Khoury-Hollins is a scientist and researcher at Waters Corporation. She holds a bachelor’s degree in science, which laid the foundation for her scientific pursuits. In 2001, Hania earned her master’s degree in Toxicology, deepening her understanding of the impact of chemicals on living organisms. In 2005 she successfully defended her Ph.D. in physical chemistry from University René Descartes - Paris V (France). After undertaking several different projects in academia Hania spent some time working at Agilent before joining Waters.At Waters, her work revolves around developing cutting-edge analytical techniques and their applications with a focus on Liquid Chromatography coupled with High-Resolution Mass Spectrometry (LC-HRMS). Her expertise encompasses a diverse range of applications, including drug development, proteomics, metabolomics, and lipidomics. More recently, Hania has been developing applications strategies for per- and polyfluoroalkyl substances (PFAS).Presenter:&nbsp;Mike McCullagh (Consulting Scientist, Waters Corporation)Michael has worked at Waters Corporation for over 23 years. His main focus has been non-targeted analysis using time of flight mass spectrometry and ion mobility mass spectrometry. Michael has made contributions to various teams, including Food and Environmental, MS Evaluation, Life Science, Scientific Operations, the Advanced Mass Spectrometry Team and currently in Research, Development and Advanced Testing. Research has been performed across multiple application areas including, impurity profiling, pesticide screening, natural products, food analysis, metabolite identification and more recently non-targeted PFAS analysis. Having worn a number of different “hats”, the common thread has been the privileged opportunity to work with new technologies, new informatics solutions and to collaborate with industry and global collaborative partners, to forge a path to new discoveries and contribute to solutions to analytical challenges across multiple disciplines.Presenter:&nbsp;Jeff Goshawk (Consulting Scientist, Waters Corporation)Jeff is a Consulting Scientist at Waters Corporation and has been with the company for twenty-four years. Jeff started his career at Waters as a software developer working on MassLynx and its associated application managers. Jeff subsequently transitioned into the applications development group where he joined the toxicology team as a Principal Scientist. Jeff is now back in the software development world at Waters, and is currently working on the Pattern Analysis Application.Presenter:&nbsp;Frank L. Dorman, PhD, FRSC (Principal Environmental Market Manager, Waters Corporation)Frank Dorman is currently Senior Business Development Manager for Waters focusing on their global environmental business and, concurrently, Associate Professor (adjunct) in the Department of Biochemistry and Molecular Biology (BMB) at Penn State University in University Park, PA. In his role at Waters, Frank manages the research-focused environmental market, external collaborations and also interfaces with R&amp;D for new product development. Prior to Waters, Frank was employed full-time as a tenure-line Faculty member of Penn State and served as a member of the Graduate Faculty in: Biochemistry, Microbiology and Molecular Biology (BMMB), Chemistry (CHEM) and the Programs in Forensics Science (FRNSC), and Biogeochemistry (BGC). Frank is also a member of the Center for Molecular Toxicology and Carcinogenesis at Penn State. Frank’s scientific interests are in gas and liquid chromatography fundamental characterization, instrumentation and column development, as well and mass spectrometry and atomic spectroscopy as applied to trace analysis in complex sample matrices. This work is directed towards environmental forensics, traditional forensic chemistry and where environmental exposure events may contribute to human disease; an area sometimes referred to as “exposomics”.Prior to joining the faculty at PSU, Frank was at Restek Corporation for 14 years as the Director of Technical Development, and R&amp;D Senior Chemist. During most of this same time, Frank was also an Adjunct Research Professor at Juniata College where he maintained an active undergraduate research program. Prior to that he was at Inchcape Testing Services for 5 years as a Senior Chemist, Organics Section Manager for Sample Preparation and Corporate Methods Development Chemist. As such, Frank has extensive experience in both the Industrial and Academic sectors giving him a relatively rare combination of skills and experience.</blockquote>

Waters Corporation: Innovative Approaches in HRMS and Software for PFAS Analysis

Innovative Approaches in HRMS and Software for PFAS Analysis

Learn how these cutting-edge approaches are revolutionizing PFAS detection and analysis, offering more accurate and efficient solutions.

<div class="summary-section section" style="-webkit-text-stroke-width:0px;background-color:rgb(255, 255, 255);box-sizing:border-box;color:rgb(51, 51, 51);font-family:&quot;Segoe UI&quot;, Frutiger, &quot;Frutiger Linotype&quot;, &quot;Dejavu Sans&quot;, &quot;Helvetica Neue&quot;, Arial, sans-serif;font-size:14px;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;font-weight:400;letter-spacing:normal;margin-bottom:34px;orphans:2;text-align:start;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:normal;widows:2;word-spacing:0px;">This interactive session will bring together the MS community to discuss some of the most popular and exciting peer-reviewed publications in a friendly and open forum. Ahead of each meeting, we will send out a link to the discussion article, then during the session an expert will summarise the main findings and answer your questions.<h5 style="margin-left:0px;">Be prepared make sure you read&nbsp;<a style="box-sizing:border-box;color:rgb(66, 139, 202);text-decoration:none;user-select:auto;" target="_blank" rel="noopener noreferrer" href="https://pubs.acs.org/doi/10.1021/acscentsci.0c00049" fr-original-style="">Native Mass Spectrometry Can Effectively Predict PROTAC Efficacy</a></h5>We're going to be joined by Dr Rebecca Beveridge from the University of Strathclyde, a senior lecturer with a host of experience with development of native ion mobility mass spectrometry methods to study proteins and protein complexes&nbsp;This paper describes how Waters' SYNAPT MS can be utilized&nbsp;demonstrate the strength of native mass spectrometry to delineate the complex binding mechanisms of protein degraders, involving the formation of three-component protein−ligand complexes.<h5 style="margin-left:0px;">Reasons to Attend:</h5><ul style="box-sizing:border-box;margin-bottom:10px;margin-top:0px;"><li style="box-sizing:border-box;">Debate some of the most topical papers from all-time favourites to some of the latest releases &nbsp;</li><li style="box-sizing:border-box;">Focus on understanding the benefit of SYNAPT MS and how to get the most out of the system for native mass spectrometry and biomolecule analysis &nbsp;</li><li style="box-sizing:border-box;">Gain a greater understanding of applications and technologies by asking questions of the authors and/or expert panel &nbsp;</li><li style="box-sizing:border-box;">No pre-recorded content, authors will be LIVE and on camera to answer questions</li></ul>Abstract&nbsp;Protein degraders, also known as proteolysis targeting chimeras (PROTACs), are bifunctional small molecules that promote cellular degradation of a protein of interest (POI). PROTACs act as molecular mediators, bringing an E3 ligase and a POI into proximity, thus promoting ubiquitination and degradation of the targeted POI. Despite their great promise as next-generation pharmaceutical drugs, the development of new PROTACs is challenged by the complexity of the system, which involves binary and ternary interactions between components. Here, we demonstrate the strength of native mass spectrometry (nMS), a label-free technique, to provide novel insight into PROTAC-mediated protein interactions. We show that nMS can monitor the formation of ternary E3-PROTAC-POI complexes and detect various intermediate species in a single experiment. A unique benefit of the method is its ability to reveal preferentially formed E3-PROTAC-POI combinations in competition experiments with multiple substrate proteins, thereby positioning it as an ideal high-throughput screening strategy during the development of new PROTACs<div style="box-sizing:border-box;clear:both;">&nbsp;</div><div class="speaker-fields-container" style="box-sizing:border-box;padding-top:5px;"><div class="section" style="box-sizing:border-box;margin-bottom:34px;"><div class="sp-begin" style="box-sizing:border-box;clear:both;color:rgb(0, 0, 0);font-size:14px;"><blockquote><div style="box-sizing:border-box;">Presenter: Dr Rebecca Beveridge (Senior Lecturer, University of Strathclyde)</div><div style="box-sizing:border-box;">&nbsp;</div><div style="box-sizing:border-box;">Rebecca is a Senior Lecturer and UKRI Future Leaders Fellow who joined the University of Strathclyde in June 2020 as a Chancellor's Fellow. She obtained a BSc in biochemistry and chemistry from the University of Leeds in 2011 and completed her PhD at the University of Manchester in 2015. Rebecca spent 4 years at the Institute for Molecular Pathology in Vienna, Austria as a postdoctoral researcher/ Lise Meitner Postdoctoral Fellow. Throughout her career, she has collected experience in a plethora of mass spectrometry methods to study protein structure such as native mass spectrometry, ion mobility, hydrogen-deuterium exchange, and crosslinking.</div>Rebecca’s current research is centered around the development of native ion mobility mass spectrometry methods to study proteins and protein complexes, particularly those that are challenging to study with conventional biochemistry methods.Such systems include intrinsically disordered proteins that exist in a wide range of different shapes, and protein complexes formed by protein-degrading molecules such as PROTACs, the complex binding modes of which are challenging to delineate.</blockquote></div></div></div></div>

Waters Corporation: Native Mass Spectrometry Can Effectively Predict PROTAC Efficacy

Native Mass Spectrometry Can Effectively Predict PROTAC Efficacy

Discuss some of the most popular and exciting peer-reviewed publications in a friendly and open forum.

Native Mass Spectrometry Can Effectively Predict PROTAC

Waters/Wyatt Technology's regional Midwest Virtual Dynamic Light Scattering (DLS) workshop is a great opportunity to learn more about DLS and latest DLS applications in the Biopharma and Gene Therapy fields. Get a refresher on biophysical characterization using dynamic light scattering, pick up some tips and tricks on DLS measurements using Wyatt instruments, learn about the capabilities and solutions beyond DLS and virtually interact with Wyatt scientists. Overall, learn how DLS can complement your existing suite of analytical tools. Registration is free for this event.This meeting will address several advanced topics presented by Wyatt scientists including:<ul><li>Determining size distributions and aggregation state using Dynamic Light Scattering (DLS)</li><li>Beyond size distributions: particle concentration, turbidity, viscosity, &amp; molecular weight</li><li>Zeta potential innovations: gentler, lower volume, better buffer compatibility with the new Wyatt Technology ZetaStar™</li><li>Overview of applications and case studies using the Wyatt DLS instruments in the biopharma and gene therapy space</li></ul>Who should attend:Active users of dynamic light scattering instruments seeking to learn more about advanced topics in dynamic light scattering physics and how it applies to our instruments and software.

Waters Corporation: Midwest Virtual Dynamic Light Scattering (DLS) Workshop

Midwest Virtual Dynamic Light Scattering (DLS) Workshop

Waters/Wyatt Technology's Dynamic Light Scattering (DLS) workshop about DLS and latest DLS applications in the Biopharma and Gene Therapy fields.

Details<ul><li>9:30-10:30 – Light scattering in the life sciences: biophysical characterization, from proteins to viruses and vesicles</li><li>10:30-10:45 – Coffee break</li><li>10:45-11:15 – Insights into the human chaperone system - Ofra Faust, Ph,D Weizmann</li><li>11:15-11:45 - Biophysical Characterization of RNA G-quadruplex and Protein Interactions - Neomi Winter, HUJI (MALS application)</li><li>11:45-12:30 – Wyatt solutions review, ZetaStar introduction</li><li>12:30-13:30 – Lunch break</li><li>13:30-14:00 - ZetaStar demo</li><li>14:00-14:30 - Interrogating biomolecular interactions with light scattering: CG-MALS</li><li>14:30-15:15 - Light scattering in pharma/biopharma: candidate screening to process analytics to stability characterization</li><li>15:15-15:30 – Coffee break</li><li>15:30-16:30 – ZetaStar playtime, Q&amp;A, one-on-one customer application review</li></ul><blockquote>Presenter: <span style="background-color:rgb(255,255,255);color:rgb(36,36,36);font-family:&quot;Segoe UI&quot;, &quot;Segoe UI Web (West European)&quot;, -apple-system, BlinkMacSystemFont, Roboto, &quot;Helvetica Neue&quot;, sans-serif;font-size:18px;"><span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;letter-spacing:normal;orphans:2;text-align:center;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:nowrap;widows:2;word-spacing:0px;">Dan Some, Ph.DPresenter: <span style="background-color:rgb(255,255,255);color:rgb(36,36,36);font-family:&quot;Segoe UI&quot;, &quot;Segoe UI Web (West European)&quot;, -apple-system, BlinkMacSystemFont, Roboto, &quot;Helvetica Neue&quot;, sans-serif;font-size:18px;"><span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;letter-spacing:normal;orphans:2;text-align:center;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:nowrap;widows:2;word-spacing:0px;">Ofrah Faust, Ph.DPresenter: <span style="background-color:rgb(255,255,255);color:rgb(36,36,36);font-family:&quot;Segoe UI&quot;, &quot;Segoe UI Web (West European)&quot;, -apple-system, BlinkMacSystemFont, Roboto, &quot;Helvetica Neue&quot;, sans-serif;font-size:18px;"><span style="-webkit-text-stroke-width:0px;display:inline !important;float:none;font-style:normal;font-variant-caps:normal;font-variant-ligatures:normal;letter-spacing:normal;orphans:2;text-align:center;text-decoration-color:initial;text-decoration-style:initial;text-decoration-thickness:initial;text-indent:0px;text-transform:none;white-space:nowrap;widows:2;word-spacing:0px;">Naomi Winter</blockquote>

Waters Corporation: Characterization of biomolecules and nanoparticles with light scattering

Characterization of biomolecules and nanoparticles with light scattering

Light scattering in the life sciences: biophysical characterization, from proteins to viruses and vesicles.