Oligonucleotide and mRNA Analysis Resource Roundup

<p><strong>What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"></p><h4><strong>A Day to Explore STEM Careers</strong></h4><p>Girls’ Future Day, widely known as <strong>Girls’ Day</strong>, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.</p><p>KNAUER has participated in Girls’ Day for <strong>17 years</strong>, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"></p><blockquote><p><i><strong>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</strong></i></p></blockquote><h4><strong>From Laboratory Instruments to a Wide Range of Careers</strong></h4><p>At <strong>KNAUER wissenschaftliche Geräte GmbH</strong>, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.</p><p>That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.</p><p>For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"></p><h4><strong>Women in Science and Technology at KNAUER</strong></h4><p>Women account for <strong>37% of KNAUER’s workforce</strong>, with particularly strong representation in scientific and management positions. The company reports a <strong>gender pay gap of 1.84% for 2026</strong>, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold <strong>44% of leadership positions</strong> at KNAUER, including several positions performed on a part-time basis.</p><p>The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.</p><p>During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"></p><h4><strong>Role Models and First-Hand Career Insights</strong></h4><p>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.</p><p>Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.</p><p>The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"></p><h4><strong>Learning HPLC by Doing</strong></h4><p>The scientific part of the day began with the basic principles of <strong>high-performance liquid chromatography (HPLC)</strong>.</p><p>To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.</p><p>One analogy compared an HPLC instrument with the human body: the <strong>pump</strong> moves liquid much like the heart circulates blood, separation takes place in the <strong>column</strong>, and the <strong>detector</strong> observes the separated compounds through their interaction with light.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"></p><h4><strong>Building a Spectroscope and Seeing Light Differently</strong></h4><p>Each participant then constructed a small handheld spectroscope.</p><p>The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.</p><p>Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.</p><p>This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"></p><h4><strong>Inside Instrument Development and Manufacturing</strong></h4><p>The program then moved from theory into KNAUER’s production environment.</p><p>In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.</p><blockquote><p><i>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</i></p></blockquote><p>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.</p><p>One detail generated particular interest: components made from <strong>sapphire and ruby</strong>. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"></p><h4><strong>Assembling a Real HPLC Pump Head</strong></h4><p>The final practical station brought the participants into instrument assembly.</p><p>Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.</p><p>With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327"></p><p>The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.</p><p>Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.</p><h4><strong>So Where Do the Cats and Rainbows Come In?</strong></h4><p>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.</p><p>When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.</p><p>The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.</p><p>Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676"></p><p>The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.</p><p>For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.</p><p>And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.</p>

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by <strong>KNAUER </strong>and<strong> Shimadzu, </strong>technical note by <strong>Thermo Fisher Scientific </strong>and poster by <strong>Waters Corporation / SOFT!</strong></p><h3><strong>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;</p><p>The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (<strong>Eurospher II 100-3 PFP column </strong>50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.</p><h4><strong>MATERIAL AND METHODS</strong></h4><ul><li><strong>Pump: </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374"><strong>P8.1L</strong></a>; high pressure gradient&nbsp;</li><li><strong>Liquid Handler:</strong> <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454"><strong>LH 8.1L </strong></a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li><strong>Thermostat: </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376"><strong>CT 2.1L&nbsp;</strong></a></li><li><strong>Detector:</strong> <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424"><strong>DAD 6.1L&nbsp;</strong></a></li><li><strong>Flow cell: </strong>Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li><strong>Software:</strong> ClarityChrom 10.1</li></ul><h4><strong>CONCLUSION&nbsp;</strong></h4><p>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.</p><h3><strong>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul><p>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;</p><p>This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.</p><h4><strong>Analysis Conditions&nbsp;</strong></h4><p>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR"><strong>Nexera XR</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050"><strong>LCMS-8050 systems</strong></a> were used.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.</p><h3><strong>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</strong></h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf"><strong>Full PDF for download</strong></a></li></ul><p>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;</p><p>To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.</p><h4><strong>Experiment</strong></h4><h5><strong>Instrumentation&nbsp;</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno"><strong>Evosep Eno&nbsp;</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959"><strong>Orbitrap Astral Zoom Mass Spectrometer&nbsp;</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464"><strong>Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</strong></a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5><strong>Software&nbsp;</strong></h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4><strong>Summary&nbsp;</strong></h4><h5><strong>Seamless and easy integration for high-throughput proteomics&nbsp;</strong></h5><p>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;</p><h5><strong>Deep proteome coverage at 500 SPD&nbsp;</strong></h5><p>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.</p><h5><strong>Reproducible quantitation across standard gradients&nbsp;</strong></h5><p>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;</p><h5><strong>Robust chromatography at ultra-high throughput&nbsp;</strong></h5><p>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;</p><h5><strong>Strong linearity for confident quantitation&nbsp;</strong></h5><p>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.</p><h3><strong>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted <strong>Tof-MRM</strong> and data-independent <strong>Tof-MSE</strong>, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148"><strong>Xevo MRT MS</strong></a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199"><strong>ACQUITY UPLC I-Class FTN PLUS System</strong></a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.</p><p>Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952"><strong>ACQUITY HSS C18 column</strong></a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.</p><p>The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from <strong>0.05 to 0.5 ng/mL</strong> in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately <strong>0.5 to 5 ng/mL</strong>. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.</p><p>Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</p></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

Article

Application

Popularization

Science and research

Poster

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

KNAUER

Tosoh Bioscience

Mettler-Toledo

Agilent Technologies

Phenomenex

Altium International (HPST)

SelectScience

SCIEX

<p><strong>In this webinar, typical LC problems are discussed - how to identify and solve them.</strong></p><blockquote><p><strong>Presenter:</strong> Ansuman Mahato (Product Specialist Liquid Phase Separations, Agilent Technologies)</p><p>Ansuman has been a Product Specialist at Agilent Technologies for 14 years, working with the pharmaceuticals, biopharmaceuticals, food, and chemicals industries. Based in Waldbronn, Germany, the manufacturing site for Agilent’s chromatography solutions, he helps make labs more efficient and compliant and has rich experience with techniques like HPLC, UHPLC, GPC/SEC, Prep LC, and 2D-LC.</p><p>Ansuman is also skilled in polymer analysis using advanced tools like viscometry and MALS. He has published many papers in international journals and is a popular speaker for his practical insights and innovative solutions.</p></blockquote>

Agilent Technologies: HPLC Beginner Webinar Series

Troubleshooting and Everyday Routine for the HPLC Instrument

In this webinar, typical LC problems are discussed - how to identify and solve them.

<p><strong>Join us on the hunt for the needle in the haystack!</strong></p><p>This session will delve into best practices for untargeted feature finding, focusing on achieving the optimal balance between sample coverage and processing speed using Profinder and the new MassHunter Explorer. We will explore techniques to differentiate between features and noise by applying the right filters for quality checking your data. Additionally, the webinar will cover essential aspects of compound identification, including database matching, formula generation, and structure elucidation.</p><p>This is a must-attend event for anyone looking to enhance their analytical capabilities and streamline their data processing workflows.</p><blockquote><p><strong>Presenter:</strong> &nbsp;Stefan Schaebler (Application Specialist LC/MS, Agilent Technologies)</p><p>Stefan completed his PhD in Biology, specializing in LC-MS-based lipidomics and metabolomics. Following his doctoral studies, he joined Agilent Technologies as an LC/MS Application Specialist. In this role, Stefan focuses on small molecule applications using LC-QTOF, with a strong emphasis on software tools and data interpretation.</p></blockquote>

Agilent Technologies: MassHunter Masterclass Months

Finding the Needle in the Haystack Part I: From Complex Samples to Compounds of Interest

The best practices for untargeted feature finding, focusing on achieving the optimal balance between sample coverage and processing speed.

From Complex Samples to Compounds of Interest

<div id="zephr-body-wall"><div><p><strong>Join Dr. Andreas Tei, Global Marketing Product Manager for Small Molecules LC &amp; GPC Columns at Agilent Technologies, as he discusses the key aspects of purification, from understanding the fundamentals of scouting techniques, to selecting the right column for your specific needs.</strong></p><p>Dr. Tei will share best practices for method optimization, offering valuable insights to enhance your purification efficiency. You will also learn about scaling up methods for larger-scale applications and discover practical tips to improve workflow efficiency.</p><p>Don’t miss this opportunity to elevate your purification strategies with expert guidance from one of the industry's leading professionals.</p><p><strong>Key learning objectives</strong></p><ul><li><strong>Scouting techniques: </strong>Understand how to explore different conditions and parameters to find the optimal purification approach</li><li><strong>Column selection:</strong> Learn how to choose the right chromatography column based on your specific needs and sample characteristics</li><li><strong>Method optimization:</strong> Discover strategies for improving purification efficiency, resolution, and purity</li><li><strong>Scaling up:</strong> Explore techniques for successful purification scale-up from lab-scale to production</li></ul><p><strong>Who should attend?</strong></p><ul><li>Users of preparative LC in drug discovery labs</li><li>Purification scientists, chemists, and lab managers trying to drive high resolution and throughput</li></ul><p><strong>Certificate of attendance</strong><br>All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</p><blockquote><p><strong>Presenter:</strong> Dr. Andreas W. Tei (Global Marketing Product Manager Small Molecules LC &amp; GPC Columns, Agilent Technologies, Inc.)</p><p>Dr. Andreas W. Tei is a marketing product manager for preparative LC columns, focusing on the integration of Agilent’s InfinityLab preparative LC columns portfolio that supports state-to-the-art purification workflows. Prior to this, he was an instrumentation product manager and led the development of Agilent’s latest generation of preparative LC instruments and automated purification software. He has a master’s degree in organic chemistry and a PhD in analytical chemistry. He has over 25 years of experience in analytical and preparative chromatography.&nbsp;</p><p><strong>Moderator:</strong> Carrie Haslam (Associate Editor, SelectScience)</p></blockquote></div></div>

SelectScience: Elevate your purification strategy with best practices for workflow efficiency and scale-up

Elevate your purification strategy with best practices for workflow efficiency and scale-up

Best practices for method optimization to enhance your purification efficiency and scaling up methods.

Best practices for purification efficiency and scale-up

<p><strong>In laboratory analysis, pH measurement is a fundamental yet often overlooked hero. However, even the most straightforward tasks can lead to unexpected pitfalls if not performed with precision.</strong></p><p>Imagine a lab where every pH reading is spot-on—analyses are consistent, reliable, and yield top-notch results. This isn’t just a dream; it can be your reality! Join our informative webinar, where we'll guide you through the essentials of pH theory and measurement processes. We’ll also provide expert tips on sensor maintenance and troubleshooting, ensuring you have all the guidance to measure pH confidently.</p><p>With a solid foundation in pH measurement practices, you’ll sharpen your skills and transform the overall quality of your results. Don’t miss this opportunity to enhance your expertise in pH measurement—let’s embark on this journey together!</p><p><strong>Event Program</strong></p><ul><li>The Fundamentals of pH: A Deep Dive into the Basics</li><li>pH Measurement Setup and Workflow: Simplifying and Streamlining Your Measurement Process</li><li>Sensor Maintenance and Troubleshooting: Optimizing Your Sensor Performance</li><li>Good Electrochemistry Practices: Elevating Your Expertise for Precise Results</li><li>Interactive Q&amp;A Session with Our Speaker</li></ul><blockquote><p><strong>Presenter:</strong> Dr. Reena Mascarenhas</p><p>Dr. Mascarenhas is a seasoned electrochemist with over 5 years of dedicated experience at METTLER TOLEDO. Her in-depth knowledge and expertise in the field have made her a sought-after speaker for live webinars. With a strong academic foundation and a passion for electrochemical advancements, Dr. Mascarenhas is committed to sharing her insights and contributing to the growth of the scientific community.</p></blockquote>

What Is pH?

Engage, Explore, and Learn the Key Concepts of pH Measurement.

<p><span><strong>Overview:</strong></span></p><ul><li>Automated clean-up using solid-phase extraction for per- and polyfluorinated alkyl substances (PFAS) (presented by Norina Aßhoff/CVUA-MEL).</li><li>PFAS method according to LOD / LOQ limits in Recommendation (EU) 2022/1431 on the monitoring of perfluoroalkyl substances in food.</li><li>In fruit, vegetables &amp; baby food: limits for PFOS: 2 ng/kg, PFOA: 1 ng/kg, PFNA: 1 ng/kg, and PFHxS: 4 ng/kg.</li><li>Validated method for animal-based foods such as eggs, milk, meat and fish.</li><li>Suitability for PFAS analysis of food contact materials made of paper.</li><li>2-SPE cartridge setup (Strata GCB 250mg + Strata-X-AW 100mg) vs. single/stacked cartridge Strata PFAS (250mg / 100mg).</li></ul><p><span><strong>Key Learning Points:</strong></span></p><ul><li>Automated sample preparation of PFAS in food, food contact materials and consumer goods.</li><li>Additional sample preparation and LC-MS/MS methods for water, soil, and blood/serum.</li><li>Blank value-reduced SPE materials and other specific laboratory accessories for PFAS analysis.</li><li>Optimization of LC-MS/MS analysis through modern columns and strategies for better retention of short-chain PFAS.</li></ul><p><span><strong>Who should attend?</strong></span></p><p>The webinar is designed for all involved in PFAS analysis in the food, feed, environmental and clinical areas, to learn about automation and new ideas for more robust PFAS metho<strong>ds.</strong></p><blockquote><p><span><strong>Presenter:</strong></span> Norina Aßhoff, CVUA-MEL</p><p><span><strong>Presenter:</strong></span> Guido Rimmel, Account Manager, Phenomenex</p><p><span><strong>Presenter:</strong></span> Georg Pfefferer, Account Manager, Phenomenex</p></blockquote><p>Please note: the webinar on PFAS will not be recorded.</p>

Phenomenex: PFAS Analysis in Food, Vegetables and Other Matrices – Automation, Hints & Developments

PFAS Analysis in Food, Vegetables and Other Matrices – Automation, Hints & Developments

Learn about automation and new ideas for more robust PFAS methods. 

PFAS Analysis in Food, Vegetables and Other Matrices

<p><strong>The technologies presented will be CE and LC-MS.</strong></p><p><i>The presentations will also be available on demand after the virtual event.</i></p><h3><strong>(Day 1): Therapeutic proteins and peptides</strong></h3><h5><strong>10:00–10:10 Welcome and introduction</strong></h5><ul><li>Steve Lock, SCIEX, UK</li></ul><h5><strong>10:10–10:35 Using LC-MS for multi-attributes monitoring (MAM) of proteins</strong></h5><ul><li>Ricardo Gomes, IBET, Portugal [NEW SPEAKER]</li></ul><h5><strong>10:35–11:00 Lastest methods for analyzing charge variants by ciEF-MS</strong></h5><ul><li>Whitney Smith, SCIEX, Germany</li></ul><h5><strong>11:00–11:25 Biomarker quantitation using LC-MS</strong></h5><ul><li>Speaker to be announced soon [NEW SPEAKERS]</li></ul><h5><strong>11:25–11:35 </strong>Break</h5><h5><strong>11:35–12:00 The lastest ways to screen N-glycans using CE</strong></h5><ul><li>Gabor Jarvas, University of Pannonia, Hungary [NEW SPEAKER]</li></ul><h5><strong>12:00–12:25 Understanding SEC and CEX coupled to LC-MS for protein intact and subunit analysis</strong></h5><ul><li>Sarah Nguyen, LSMBO, Strasbourg University, France</li></ul><h5><strong>12:25–12:50 Beneath the peak: mass spectrometry as a tool in ADC development</strong></h5><ul><li>Christopher Nortcliffe, Sterling Pharma Solutions, UK</li></ul><h5><strong>12:50–13:00</strong> Wrap-up and Q&amp;A</h5><blockquote><p><strong>Your host: </strong>Steve Lock, PhD (Senior Marketing and Market Development Manager, Biopharma, EMEAI, SCIEX)<br><br>Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world. He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.</p></blockquote>

2024 SCIEX Biopharma meeting (Day 1): Therapeutic proteins and peptides

This online event has been designed for our customers and brings  together opinion leaders, top scientists and industry innovators.

2024 SCIEX Biopharma: Therapeutic proteins and peptides

<p><strong>The technologies presented will be CE and LC-MS.</strong></p><p><i>The presentations will also be available on demand after the virtual event.</i></p><h3><strong>(Day 2): Gene therapy, vaccines and oligonucleotides</strong></h3><h5><strong>10:00–10:10 Welcome and introduction</strong></h5><ul><li>Steve Lock, SCIEX, UK</li></ul><h5><strong>10:10–10:35 The latest developments in plasmid analysis by CE</strong></h5><ul><li>Luiza Chrojan / Ryan Hylands, Pharmaron, UK</li></ul><h5><strong>10:35–11:00 Pure and simple: understanding LNP analytics for better mRNA-based drugs</strong></h5><ul><li>Adam Crowe, Cytiva, Canada</li></ul><h5><strong>11:00–11:25 Tips and tricks to speed up CE-SDS analysis</strong></h5><ul><li>Marcus Heinl, SCIEX, Germany</li></ul><h5><strong>11:25–11:35</strong> Break</h5><h5><strong>11:35–12:00 Applying CE to viral vector analysis of process samples</strong></h5><ul><li>Juliette Laigle / Yolande Masson, Yposkesi, France [NEW SPEAKERS]</li></ul><h5><strong>12:00–12:25 Exploring mRNA mapping and aggregation analyses for mRNA characterization</strong></h5><ul><li>Lucia Geis Asteggiante, Phenomenex, Italy</li></ul><h5><strong>12:25–12:50 After the experiment: electronic lab execution as standard</strong></h5><ul><li>Marc Smith, IDBS, UK [NEW SPEAKER]</li></ul><h5><strong>12:50–13:00</strong> Wrap-up and Q&amp;A</h5><blockquote><p><strong>Your host: </strong>Steve Lock, PhD (Senior Marketing and Market Development Manager, Biopharma, EMEAI, SCIEX)<br><br>Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world. He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.</p></blockquote>

2024 SCIEX Biopharma meeting (Day 2): Gene therapy, vaccines and oligonucleotides

SCIEX Biopharma: Gene therapy, vaccines and oligonucleotides

<p><strong>This presentation features the work of Robbin Bouwmeester, a senior postdoc in the CompOmics group in Ghent, Belgium, who completed his PhD in 2020. Robin is an expert in artificial intelligence and retention time prediction. His presentation, titled "Improvise, Adapt and Overcome," focuses on retention time prediction in the context of proteins and biomolecules.</strong></p><p>He discusses the importance of identifying and quantifying biomolecules in cells, and the use of graph networks to improve prediction accuracy. Robbin highlights the benefits of separating amino acids as individual graphs and combining them with code enrollments, resulting in an 11% performance improvement.</p><p>He also touches on the use of chemical descriptors and graph representations to better distinguish peptides, and the potential of training on broader peptide sets before fine-tuning to smaller, more complex data sets. Additionally, he showcases a successful example of accounting for modifications in retention time prediction with high correlation coefficients.&nbsp;</p><h5>Learning points:</h5><ul><li id="isPasted"><strong>Importance of Transfer Learning&nbsp;</strong></li><li><strong>Role of Modifications in Retention Time Prediction&nbsp;</strong></li><li><strong>Application of Machine Learning &nbsp;</strong></li></ul><h5>Who should attend:</h5><ul><li>Researchers and professionals in the fields of bioinformatics, computational biology, and analytical chemistry, particularly those who are involved in proteomics and the study of biomolecules&nbsp;</li><li>Individuals working with machine learning applications in biological sciences or those interested in retention time prediction and data-driven approaches to understanding biomolecular behavior would find the presentation valuable.</li></ul><p><strong>Presenter:</strong> Robbin Bouwmeester (CompOmics Group, Ghent University/VIB, Belgium)</p>

Thermo Fisher Scientific: Improvise, Adapt and Overcome: retention time prediction in the context of proteins and biomolecules

Improvise, Adapt and Overcome: retention time prediction in the context of proteins and biomolecules

Robin discusses the importance of identifying and quantifying biomolecules in cells, and the use of graph networks to improve prediction accuracy.

Retention time prediction in the context of proteins

<p><strong>Celebrating 50 years of chromatography evolution, we have embarked on an incredible journey together, sharing extensive knowledge in liquid chromatography, understanding your insights, and continuously evolving.</strong></p><p>Agilent is committed to fostering strong customer connections and adopting a focused approach in designing HPLC instruments for today and the future. Stepping into the future, we are delighted to invite you to experience the latest advancement in chromatography from Agilent: Infinity III Series, the HPLC Made for Tomorrow.</p><h5><strong>We are going to give you an exclusive sneak peek into the newly launched Infinity III LC Series:</strong></h5><p><strong>1. Infinity III LC Series Overview</strong></p><p><strong>2. Infinity Lab Assist Lab-Demo Video</strong></p><p><strong>3. Q&amp;A Session about the new Infinity III LC Series with experts - Jens Meixner and Florian Rieck</strong></p><p>Register now and encourage your industry colleagues to join this launch webinar.</p><div dir=""><div><blockquote><div><strong>Presenter:</strong> Jens Meixner, PhD (Product Manager, Agilent Technologies)</div></blockquote></div><div><blockquote><p><span>Jens Meixner is a product manager at Agilent Technologies, where he oversees the analytical core HPLC and CE-MS portfolios. He began his career in 2014 as a PhD student at Aalen University and the University of Tübingen, where he studied analytical chemistry. During his Ph.D. he focused on the development of multidimensional separation techniques like CExCE and LCxCE coupled with high-end MS detection for the characterization of therapeutic drugs.</span></p></blockquote><div dir=""><div><blockquote><div><strong>Presenter:</strong> Florian Rieck, PhD (Scientist Application Development, Agilent Technologies)</div></blockquote></div><div><blockquote><p align="LEFT"><span>Florian Rieck holds a master's degree in food chemistry and a PhD in analytical chemistry from the Karlsruhe Institute of Technology (KIT) in Germany. Florian joined Agilent Technologies in 2015 as an applications chemist for liquid chromatography. An author of numerous application notes covering different analytes and detection techniques, he has also been responsible for a number of customer collaborations and demonstrations of the Agilent InfinityLab LC portfolio.</span></p></blockquote></div></div></div></div>

Agilent Technologies: Agilent Infinity III LC Series

Agilent Infinity III LC Series

Stepping into the future, we are delighted to invite you to experience the latest advancement in chromatography from Agilent: Infinity III Series.

<p><strong>Christian Rohrer, Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH, will present the next edition of Tosoh Tuesday, a webinar series held by Chromatography Experts.&nbsp;</strong></p><p>On Tuesday, November 19, Snežana Đorđević, Application Scientist at Tosoh Bioscience, will demonstrate the SEC-MALS potential in unraveling the structural information of biopolymers. Learn about the importance of implementing accurate and fit-for-purpose analytical characterization steps from the start and throughout the design process of polymer therapeutics.</p><p><strong>After the webinar, ask your questions during the live Q&amp;A session.&nbsp;</strong></p><blockquote><p><strong>Presenter: </strong>Christian Rohrer (Director of Sales &amp; Support – EMEA at Tosoh Bioscience GmbH)</p><p><strong>Presenter:</strong> Snežana Đorđević (Application Scientist at Tosoh Bioscience)</p></blockquote>

Tosoh Bioscience: Tosoh Tuesday - Advanced Characterization of Polymers by SEC-MALS

Tosoh Tuesday - Advanced Characterization of Polymers by SEC-MALS

Learn how SEC-MALS provides structural insights into biopolymers and supports accurate analytical characterization in polymer therapeutic development.