Oligonucleotide and mRNA Analysis Resource Roundup

What do chromatography, gemstones, rainbows and even cats have in common? At KNAUER’s latest Girls’ Day in Berlin, 15 schoolgirls explored exactly that question. Through a series of hands-on activities, they discovered how HPLC instruments are developed and manufactured, learned the basics of spectroscopy and chromatography, and met women working across science, engineering, product management and company leadership.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg" alt="KNAUER: Figure 1 A student observes the rainbow spectrum of white light." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_A_student_observes_the_rainbow_spectrum_of_white_light_8ef24d9700.jpeg 228w," sizes="100vw" width="624" height="427"><h4>A Day to Explore STEM Careers</h4>Girls’ Future Day, widely known as Girls’ Day, gives schoolgirls across Germany an opportunity to experience professions in which women are still underrepresented. Instead of learning about careers only in the classroom, participants can spend a day inside companies, laboratories and technical workplaces and see what these jobs actually involve.KNAUER has participated in Girls’ Day for 17 years, and demand for places has become consistently high. The attraction is easy to understand: visitors do not simply listen to presentations. They can experiment, assemble components, talk directly with employees and discover how science and engineering are applied in everyday work.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg" alt="KNAUER: Figure 2 - Hands-on experience and wonder at Girls’ Day 2025: 13 interested girls assembled pump heads, learned what HPLC is, and how such equipment is developed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Hands_on_experience_and_wonder_at_Girls_Day_2025_98da525b3a.jpg 125w," sizes="100vw" width="1024" height="1280"><blockquote>“That moment when the girls realize, with sparkling eyes, just how cool science can be—that’s exactly why I love Girls’ Day. It’s worth every bit of the effort!” – Dr. Kristin Folmert, chemist and product manager at KNAUER</blockquote><h4>From Laboratory Instruments to a Wide Range of Careers</h4>At KNAUER wissenschaftliche Geräte GmbH, laboratory instrumentation is designed, developed, manufactured, tested and marketed from Berlin for customers around the world.That requires far more than chemists alone. The company brings together people working in mechanical production, logistics, media design, mechatronics, software development, science, product management and company management.For the participating girls, this offered a chance to meet women following very different career paths and to see that a technology company can provide opportunities across many disciplines.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg" alt="KNAUER: Figure 3 - The girls learned what chromatography and HPLC are, what they are used for, and how a UV/Vis detector works. They then built their own handheld spectroscope and experimented with white light and various color filters." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_The_girls_learned_what_chromatography_and_HPLC_are_a7db4e8eb3.jpg 245w," sizes="100vw" width="1024" height="609"><h4>Women in Science and Technology at KNAUER</h4>Women account for 37% of KNAUER’s workforce, with particularly strong representation in scientific and management positions. The company reports a gender pay gap of 1.84% for 2026, compared with an unadjusted German gender pay gap of 16% in 2025. Women also hold 44% of leadership positions at KNAUER, including several positions performed on a part-time basis.The company emphasizes practical measures intended to make technical careers accessible to a wider range of employees. These include flexible work models, support for childcare and technical solutions such as indoor cranes for handling heavy components.During Girls’ Day, these policies become less abstract. Participants meet scientists, engineers and managers who can describe how they entered their professions and how their careers developed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg" alt="KNAUER: Figure 4 - During a visit to the equipment development department, scientist Thy Vu talks about her career at KNAUER. She wrote her bachelor’s thesis here and then worked as a student assistant in development during her master’s program. Subsequent to completing her master’s degree, she accepted a permanent position at KNAUER in the development department. Today, she is developing the pump drive of the future and tells the fascinated girls which gemstones are used in such a pump (sapphires and rubies)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_4_During_a_visit_to_the_equipment_development_department_scientist_Thy_Vu_talks_about_her_career_at_KNAUER_3bd9af2786.jpg 245w," sizes="100vw" width="1024" height="614"><h4>Role Models and First-Hand Career Insights</h4>Several KNAUER employees are also involved in <a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/groups/13080121/">International Women in Chromatography</a>, a network supporting women and non-binary professionals working in chromatography.Throughout the day, the students encountered women at different stages of their careers: from university students and young scientists to experienced product managers, developers and Managing Director Alexandra Knauer.The message was not simply that STEM careers are available to women, but that scientific and technical work can take many forms. Networking, curiosity, willingness to ask questions and confidence in one’s own abilities were recurring themes throughout the event.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg" alt="KNAUER: Figure 5 - A girl works intently and with great care on her spectroscope. None of the girls glance at their phones; they are all polite, and many feel comfortable asking questions openly. We are very excited about this generation of bright young talents." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_5_A_girl_works_intently_and_with_great_care_on_her_spectroscope_ed94a2a049.jpg 127w," sizes="100vw" width="405" height="496"><h4>Learning HPLC by Doing</h4>The scientific part of the day began with the basic principles of high-performance liquid chromatography (HPLC).To make separation easier to understand, the group first experimented with light and heavy balls inside a large glass column. From there, the discussion moved to the individual components of an HPLC system.One analogy compared an HPLC instrument with the human body: the pump moves liquid much like the heart circulates blood, separation takes place in the column, and the detector observes the separated compounds through their interaction with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg" alt="KNAUER: Figure 6 - The HPLC and its various modules, depicted as human organs." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_6_The_HPLC_and_its_various_modules_depicted_as_human_organs_855ab67aac.jpg 245w," sizes="100vw" width="1210" height="670"><h4>Building a Spectroscope and Seeing Light Differently</h4>Each participant then constructed a small handheld spectroscope.The task required both careful reading and manual dexterity, particularly when fitting the delicate lens. Working together, the girls quickly developed better ways to complete the assembly—a small example of how real development projects evolve through cooperation and continuous improvement.Once finished, the spectroscopes revealed the spectrum of white light as a rainbow together with visible spectral features. Colored filters placed between the light source and the spectroscope then demonstrated how absorption changes the observed spectrum.This connected the practical activity with concepts introduced earlier in the day: visible light, wavelength, absorption and how optical signals eventually become peaks in HPLC detection.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg" alt="KNAUER: Figure 7 - A 11-year-old girl gazes fascinated at her rainbow and notices that while all the colors are visible, some areas are brighter while others are quite dark. “Is the spotlight not pure white, or is my spectroscope absorbing light?” Such clever questions arise when girls are encouraged to explore things for themselves." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_7_A_11_year_old_girl_gazes_fascinated_at_her_rainbow_4f713de2ae.jpg 200w," sizes="100vw" width="1024" height="800"><h4>Inside Instrument Development and Manufacturing</h4>The program then moved from theory into KNAUER’s production environment.In the mechanical manufacturing area, participants saw CNC machines and robotic systems producing precision components for laboratory instruments. They could watch parts of an HPLC pump head taking shape directly from metal components and learn why accurate machining is essential for reliable instrument performance.<blockquote>“This is like science fiction!” – Mariko Stahr, Girls’ Day participant</blockquote>In the development department, engineer Thy Vu demonstrated her work on pump-drive technology and explained how developers investigate parameters such as motor torque. The girls were also able to handle some of the materials used inside an HPLC pump.One detail generated particular interest: components made from sapphire and ruby. This opened a discussion about material properties, crystal structures and why instrument developers benefit from understanding chemistry as well as engineering.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg" alt="KNAUER: Figure 8 - Thomas Müller explains to the girls how CNC machines mill the housing for a pump head from a block of metal and why precision is crucial in this process. On the left is one of the robotic arms that continues working even at night, allowing employees to focus solely on daytime work and more demanding tasks. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_8_Thomas_Mueller_explains_to_the_girls_how_CNC_machines_mill_the_housing_for_a_pump_head_bd714b9b50.jpg 245w," sizes="100vw" width="1278" height="468"><h4>Assembling a Real HPLC Pump Head</h4>The final practical station brought the participants into instrument assembly.Each girl was given the opportunity to assemble a pump head herself. The work proved more demanding than it initially appeared, requiring both hands to coordinate several components accurately.With guidance from KNAUER training supervisor Ronald Berger, the participants completed their assemblies before each pump head underwent a final technical check to verify that the pistons moved correctly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg" alt="KNAUER: Figure 9 - A girl is screwing the check valve with the check valve holder into the pump head. Everything is then tightened using a torque wrench." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_9_A_girl_is_screwing_the_check_valve_with_the_check_valve_holder_i_cf84fb4375.jpg 179w," sizes="100vw" width="376" height="327">The day concluded with lunch and another opportunity to speak with Managing Director Alexandra Knauer, who has long supported initiatives intended to improve the visibility of women and encourage young people to consider STEM careers.Participants received certificates and “I love HPLC” bags with small gifts before leaving KNAUER with their own impressions of what a future in science and technology could look like.<h4>So Where Do the Cats and Rainbows Come In?</h4>The rainbow seen through a spectroscope provides a simple demonstration of the same basic optical principle used in many analytical measurements.When white light is dispersed, its different wavelengths become visible as a spectrum. If part of that light is absorbed by an object or substance, the resulting spectrum changes. Measuring which wavelengths are absorbed can therefore reveal information about the material interacting with the light.The article illustrates the idea with a deliberately simple example: a cat sitting in front of a light source may absorb most of the light, but its silhouette still provides information about what is blocking the beam.Similarly, analytical detectors can obtain information about molecules by measuring how they interact with light.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg" alt="KNAUER: Figure 10 - The cat actually absorbs all the light, but its outline still lets us know that there’s a fluffy cat sitting there. In HPLC, a[SD5.1] molecule’s light absorption also tells us a great deal about its identity. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_10_The_cat_actually_absorbs_all_the_light_af8452f58d.jpg 236w," sizes="100vw" width="1024" height="676">The same principle also helps explain the colors of evening sunlight. When sunlight travels through a longer path in the atmosphere, shorter-wavelength blue light is scattered more strongly, leaving proportionally more of the longer-wavelength red and orange light reaching the observer.For the students at KNAUER, concepts such as these transformed HPLC and spectroscopy from abstract scientific terminology into something they could build, observe and discuss themselves.And that is ultimately the purpose of Girls’ Day: to show young people that science and technology are not distant subjects confined to textbooks, but fields full of practical questions, creative problem solving and possible future careers.

Girls Just Wanna Have Fun With KNAUER – A Girls' Day Full of Rainbows

Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 21st September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!<blockquote>👉 <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library">SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</a></blockquote><blockquote>👉 Need info about different analytical techniques? Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library">LabRulezGCMS</a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library">LabRulezICPMS</a> libraries.</blockquote>This week we bring you application notes by KNAUER and Shimadzu, technical note by Thermo Fisher Scientific and poster by Waters Corporation / SOFT!<h3>1. KNAUER: Precise Catechin Profiling in Various Types of Tea</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0198_knauer_precise_catechin_profiling_in_various_types_of_tea_d607cde5ba.pdf">Full PDF for download</a></li></ul>Fresh tea leaves contain high concentrations of catechins, which have many beneficial properties. These include antioxidant, anti-inflammatory as well as antimicrobial effects, and it is even claimed that they have the potential to prevent cancer [1]. In addition, the taste and mouthfeel of a tea strongly depend on its catechin profile. The catechin content of tea leaves varies based on cultivation area, age and variety, as well as on how they are fermented, processed and packed. Therefore, the catechin content is an important marker for the quality of tea.&nbsp;The analysis of catechins can be challenging. The structural similarity of the catechin isomers and other closely related compounds result in similar behavior and a difficult separation (Fig. 1). Catechins possess many phenolic hydroxyl groups, which are prone to secondary interactions, like hydrogen bonding. Classical as well as highly endcapped C18 columns offer only limited retention and selectivity for catechins. Therefore, the KNAUER column portfolio has been expanded to include a pentafluorophenyl (PFP)-modified stationary phase (Eurospher II 100-3 PFP column 50 x 2 mm). It offers optimal separation of the catechins based on hydrophilic, hydrophobic and π-π-interactions. The column withstands acidic eluents (pH 1.5–10) as well as 100 % aqueous conditions, which are beneficial for the retention of hydrophilic compounds. The KNAUER Gradient Prediction Tool assisted with the challenging method development which is described in detail in the TechNote “The Gradient Prediction Tool: Method Development for the Separation of Catechins from Tea”. Here, the application of the optimized method for the identification and quantification of catechins in tea extracts is shown. The seven compounds specified in ISO-14502-2 (2005), as well as two additional catechins known as (+)-gallocatechin and (–)-gallocatechin gallate, were quantified.<h4>MATERIAL AND METHODS</h4><ul><li>Pump: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2374">P8.1L</a>; high pressure gradient&nbsp;</li><li>Liquid Handler: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1454">LH 8.1L </a>With FastWash station, and Robotic cooler. Loop: 20 µl.</li><li>Thermostat: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2376">CT 2.1L&nbsp;</a></li><li>Detector: <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2424">DAD 6.1L&nbsp;</a></li><li>Flow cell: Analytical LightGuide flow cell (50 mm, 6 µl)&nbsp;</li><li>Software: ClarityChrom 10.1</li></ul><h4>CONCLUSION&nbsp;</h4>The application note demonstrates the reliable separation and quantification of catechins and caffeine in tea extracts using the new KNAUER PFP column. Using an optimised gradient predicted with the KNAUER Gradient Prediction Tool, nine tea-related compounds were separated in under 23 minutes. The tested tea samples showed different catechin content highlighting how strongly tea composition depends on the product type and processing.<h3>2. Shimadzu: New Derivatization Method for Quantitative Analysis of Δ⁹-THC-COOH in Urine</h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01215_en_c98174629b.pdf">Full PDF for download</a></li></ul><h5>User Benefits</h5><ul><li>The risk of limited availability of stable isotope-labeled standards can be avoided by synthesizing internal standards through derivatization.&nbsp;</li><li>The derivatizing agent IPPAH and its corresponding stable isotope IPPAH-d6 can be synthesized easily and inexpensively from acetone (or acetone-d6).&nbsp;</li><li>This approach achieves sufficient sensitivity and quantitative accuracy for the determination of Δ⁹-THC-COOH in urine.</li></ul>In Japan, the Cannabis Control Act and the Narcotics and Psychotropics Control Act were amended in December 2024. While cannabis was legalized for industrial and medical uses, stricter cannabis regulations have been enacted to prevent harm from its abuse. The primary psychoactive cannabinoid, delta-9-tetrahydrocannabinol (Δ⁹-THC), is extensively metabolized in the body and is excreted mainly in urine and feces as 11-nor-9-carboxy-THC (Δ⁹-THC-COOH) and its glucuronide conjugates. Consequently, Δ⁹-THC-COOH must be analyzed to confirm cannabis use. For LC-MS/MS quantitation, the stable isotope-labeled internal standard Δ⁹-THC-COOH-d3 is commonly used to correct for matrix effects. However, most suppliers of stable isotope standards are located in the U.S., making these standards difficult to obtain due to import procedures. Furthermore, there may be additional importrelated supply risks for stable isotope standards in the future.&nbsp;This Application News describes a technique for quantitating Δ⁹-THC-COOH using an internal standard that is synthesized easily and inexpensively by derivatization with IPPAH (1- isopropylpiperidine carboxylic acid hydrazide), which characteristically reacts with carboxyl groups, and its corresponding stable isotope IPPAH-d6.<h4>Analysis Conditions&nbsp;</h4>LC-MS/MS analysis conditions are shown in Table 1. The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+XR">Nexera XR</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=LCMS-8050">LCMS-8050 systems</a> were used.<h4>Conclusion&nbsp;</h4>Derivatization with IPPAH and its stable isotope analog, IPPAHd6, enabled the simple and inexpensive preparation of an internal standard. This approach provides sufficient sensitivity and accuracy for the determination of Δ⁹-THC-COOH in urine. Most stable isotope-labeled standards are supplied by foreign manufacturers, creating a risk of delays or difficulties in procurement due to import/export regulations. By synthesizing the internal standard through derivatization, this risk can be minimized.<h3>3. Thermo Fisher Scientific: High-throughput quantitative proteomics with Evosep Eno and the Orbitrap Astral Zoom Mass Spectrometer</h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004544_ov_astralzoom_evosepeno_htp_proteomics_tn004544_na_en_f31db9084e.pdf">Full PDF for download</a></li></ul>High-throughput proteomics is a critical enabling technology for large clinical studies, high-content drug screening, and systems biology research, allowing for comprehensive and quantitative analysis of complex proteomes across large sample cohorts within practical timeframes. As biological and translational studies expand in scale and complexity, the ability to generate deep and reproducible proteome measurements across thousands of samples has become increasingly essential for robust biomarker discovery, network-level systems modeling, and downstream analyses. In these contexts, accurate and precise protein quantitation must be maintained without sacrificing throughput, placing stringent demands on the performance and robustness of both LC instruments and mass spectrometers.&nbsp;To meet these increasing analytical demands, the Evosep Eno was developed as a high‑throughput LC system that combines robustness, ease of use, and highly reproducible chromatography. It offers six standardized methods: 30, 60, 100, 200, 300, and 500 samples per day (SPD), allowing users to select the throughput that aligns best with their study goals.1 These predefined methods ensure consistent gradient delivery, highly stable retention times, and reproducible system performance across instruments and laboratories.2 By reducing LC‑related variability and simplifying everyday operation, the Evosep Eno offers a strong and consistent foundation for large‑scale proteomics workflows, particularly those relying on short gradients and high reproducibility required for downstream quantitative analysis.<h4>Experiment</h4><h5>Instrumentation&nbsp;</h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=291&amp;search=evosep+eno">Evosep Eno&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1959">Orbitrap Astral Zoom Mass Spectrometer&nbsp;</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2464">Thermo Scientific™ EASY-Spray™ Ion Source&nbsp;</a></li><li>Evosep™ Pod for EASY-Spray Ion Source</li></ul><h5>Software&nbsp;</h5><ul><li>Biognosys Spectronaut® 19.7 software&nbsp;</li><li>MacCoss Lab Software Skyline-Daily V25.1</li></ul><h4>Summary&nbsp;</h4><h5>Seamless and easy integration for high-throughput proteomics&nbsp;</h5>Evosep Eno integrates seamlessly with the Orbitrap Astral Zoom Mass Spectrometer, supported by EASY-Spray source-compatible columns, emitters, and the Evosep Pod column oven for a simple, streamlined, high-throughput workflow.&nbsp;<h5>Deep proteome coverage at 500 SPD&nbsp;</h5>Optimized MS parameters for the standard 500 SPD Evosep Eno gradient enabled identification of more than 6,500 protein groups and 71,000 modified peptides from three replicates of 200 ng Pierce HeLa Digest/PRTC Standard.<h5>Reproducible quantitation across standard gradients&nbsp;</h5>Across all standard Evosep Eno gradients, the workflow delivered highly reproducible label-free quantification, with median protein group %CVs at or below 5.2%, while identifying more than 10,500 protein groups and 190,000 modified peptides from five replicates of 200 ng Pierce HeLa Digest/PRTC Standard at 30 SPD.&nbsp;<h5>Robust chromatography at ultra-high throughput&nbsp;</h5>Chromatographic performance remained stable and robust at 500 SPD, with nearly 100 consecutive runs showing PRTC retention time standard deviations below 0.4 seconds and a median of 6 DPPP.&nbsp;<h5>Strong linearity for confident quantitation&nbsp;</h5>Quantitative performance remained strong at 500 SPD, with PRTC peptides showing excellent linearity across the active gradient (R² &gt;0.98) when spiked from attomolar to femtomolar concentrations.<h3>4. Waters Corporation / SOFT: COMPARATIVE EVALUATION OF HIGH RESOLUTION MASS SPECTROMETRY ACQUISITION STRATEGIES FOR THE DETECTION AND ANALYSIS OF ILLICIT DRUG SUBSTANCES</h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009592_0bd2b5ee6a.pdf">Full PDF for download</a></li></ul><div><div><section><div><div><div><div><div><div>The poster compares two high-resolution time-of-flight mass spectrometry acquisition strategies for toxicology screening: targeted Tof-MRM and data-independent Tof-MSE, both implemented on a Waters <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1148">Xevo MRT MS</a> coupled to an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/199">ACQUITY UPLC I-Class FTN PLUS System</a>. The aim was to determine whether these approaches could meet routine service requirements for drug testing while also broadening the number of substances that can be detected beyond conventional targeted panels. Urine samples were prepared across a wide concentration range for 20 target analytes, and the methods were also applied to 20 anonymized authentic urine samples.Both workflows used a 15-minute LC separation on an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952">ACQUITY HSS C18 column</a> under positive electrospray ionization. In the targeted Tof-MRM mode, selected precursor ions were isolated and fragmented, with quantifier and qualifier ions monitored for sensitive quantitative analysis. In contrast, Tof-MSE operated in a data-independent mode, alternating between low and elevated collision energies to collect accurate-mass precursor and fragment-ion information for a broad range of compounds. Data were processed in the waters_connect platform using MS Quan for Tof-MRM and UNIFI with the Waters Toxicology Library, containing more than 2,100 drugs and metabolites, for Tof-MSE.The targeted Tof-MRM method provided the highest sensitivity, with limits of detection ranging from 0.05 to 0.5 ng/mL in fortified urine. The broader Tof-MSE workflow achieved LODs of approximately 0.5 to 5 ng/mL. When both strategies were applied to authentic urine samples, they showed excellent qualitative agreement. Importantly, Tof-MSE detected additional compounds outside the predefined target panel, including commonly encountered medications, benzodiazepines, antidepressants, and illicit drug substances that would not have been captured by the targeted method alone.Overall, the study shows that Tof-MRM can deliver sensitivity comparable to conventional tandem-quadrupole LC-MS/MS for targeted urinary drug analysis, while Tof-MSE adds broader sample characterization and retrospective screening capability. Used together, the two acquisition strategies provide a flexible approach that combines sensitive quantification of predefined analytes with expanded screening for unexpected or previously untargeted drug substances in complex biological samples.</div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 39, 2026

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Shimadzu Corporation

Waters Corporation

In a typical laboratory, quality control mishaps are inevitable during sample preparation. For example, a sample going missing or a sample being miss-identified will result in downstream effects including the loss of significant time, money and samples. Therefore, advances in digitalization have been shown to reduce human error as manual input and transcription is eliminated from the process. Consequently, this lowers the likelihood of obstacles such as these, to improve data accuracy and process efficiency.Join Dr. Kelsey Mato, Product Marketing at Agilent Technologies, to hear about a new technology, linking samples and software for unprecedented control, to give you confidence in your laboratory workflow.Key learning objectives:<ul><li>Understand the long-term risks and costs of manual sample tracking</li><li>Learn how taking steps toward digitalization can reduce errors in sample management</li><li>Find a new solution for seamlessly linking samples and software</li></ul>Who should attend?Lab managers, and experienced and inexperienced lab technicians.Certificate of attendanceAll webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.<blockquote>Presenter: Dr. Kelsey Mato (Product Marketing, Agilent Technologies)Dr. Kelsey Mato completed her PhD in analytical chemistry at Louisiana State University. Her career began focusing on sensing devices in the biopharmaceutical industry as well as her involvement in software for a wide range of instruments. Ensuing this propelled her efforts to specialize in analytical instrumentation where currently she is working as a product marketing professional at Agilent Technologies.Presenter: Olivia Long (Editorial Team, SelectScience)</blockquote>

Agilent Technologies: Eliminate errors with automation and stress-free sample tracking

Eliminate errors with automation and stress-free sample tracking

Join us to hear about a new technology, linking samples and software for unprecedented control, to give you confidence in your laboratory workflow.

Eliminate errors with automation and easy sample tracking

<h4>Eight Webinars and a Roundtable with Experts from Merck&nbsp;</h4>Analytical Excellence Week' is organized to empower professionals in the field of analytical chemistry. Over the course of eight insightful sessions, industry experts will share their knowledge and best practices to streamline your analytical workflows and enhance your efficiency.From understanding quality grades in inorganics and solvents to exploring sustainable practices in the lab, each webinar covers essential topics that will enhance your analytical capabilities. Learn how to optimize your Karl Fischer titration, achieve precision in PFAS testing, and gain troubleshooting tips for LC-MS to effectively address common challenges and improve your laboratory results. The sessions will also address critical aspects of safety and sustainability, ensuring you are well-equipped to handle hazardous chemicals responsibly while making strides toward greener laboratory practices.This is an invaluable opportunity to gain insights that can significantly optimize your analytical processes. Don’t miss your chance to elevate your expertise and contribute to a more efficient and sustainable future in analytical workflows. Each session is specifically designed to address essential topics that directly impact your daily practices, making it relevant for all professionals working in a chemical analysis laboratory.<h3>Day 2: Tuesday, 11 February</h3><h4>Dihydropinene, γ-Valerolactone, and Dimethyl Isosorbide: Bio-Sourced Versatile Green Solvents for Diverse Organic Synthesis</h4>The growing environmental concerns and regulatory restrictions on conventional organic solvents derived from non-renewable sources have spurred the search for greener, sustainable alternatives. This presentation will explore the potential of dihydropinene (DHP), γ-valerolactone (GVL), and dimethyl isosorbide (DMI) as bio-sourced and biodegradable solvents, offering a promising solution to replace toxic and non-renewable solvents. DHP, derived from turpentine oil, is an effective substitute for solvents such as n-hexane, benzene, and dichloromethane, while GVL and DMI are ideal replacements for hazardous solvents such as DMF, NMP, and DMA.These solvents not only perform well in various chemical transformations but also offer significant environmental benefits due to their biodegradability, low toxicity, and recyclability. The presentation will highlight the applications of DHP, GVL, and DMI in green chemistry, showcasing their potential in organic synthesis and industrial processes. It will also discuss their role in advancing sustainable practices, reducing dependency on fossil-based solvents, and promoting safer working environments. By focusing on green solvent solutions, the presentation will emphasize their future potential in scientific research, production, and industrial applications.By attending this webinar, you will learn:<ul><li>Sustainable Solvent Alternatives: How bio-based solvents such as DHP, GVL, and DMI can replace conventional toxic solvents, providing eco-friendly, biodegradable options for chemical processes.</li><li>Environmental and Safety Benefits: The environmental advantages of these solvents, including biodegradability, low toxicity, and recyclability, and how they promote safer and greener practices in research and industry.</li><li>Practical Applications: How these solvents can be effectively used in organic synthesis, industrial processes, and various scientific fields, contributing to more sustainable and efficient chemical solutions.</li></ul>Who should attend: This presentation will benefit those working in pharmaceutical and drug discovery R&amp;D, process development of API, environmental scientists and sustainability experts, industry professionals in chemical engineering and manufacturing, materials science researchers, and students and researchers in green chemistry.<blockquote>Presenter: Saikat Sinha, (R&amp;D Manager, Merck)&nbsp;</blockquote><h4>Enhance Your PFAS Testing: Achieve Precision and Reduce Background Noise with Specialized Consumables</h4>Join this presentation to explore advanced LC-MS/MS methods for determining PFAS analytes at low levels across diverse samples and matrices. Learn how to identify contamination sources, reduce background noise, and improve testing precision with specific recommendations on PFAS-qualified consumables. Discover various LC-MS/MS PFAS testing workflows and strategies to minimize errors, supported by data from both regulated and non-regulated methods.By attending this webinar, you will learn:<ul><li>Detailed insights into different LC-MS/MS PFAS methods</li><li>Techniques to identify and test contamination sources</li><li>Strategies to reduce background noise</li><li>Practical tips and tricks for improved testing</li></ul>Who should attend: This webinar is essential for lab technicians, lab managers, and quality assurance managers involved in developing in-house methods or verifying regulated testing protocols such as EPA Methods 533, 537.1, and 1633.<blockquote>Presenter: Patrik Appelblad, (Fellow and Technical Lead, Analytical Chemistry R&amp;D, Merck)&nbsp;</blockquote>

Merck: Webinar Series & Expert Roundtable

Webinar Series & Expert Roundtable (Day 2)

(Day 2) - Part 1: Dihydropinene, γ-Valerolactone, and Dimethyl Isosorbide: Solvents for Organic Synthesis and Part 2: Enhance Your PFAS Testing

<h4>Eight Webinars and a Roundtable with Experts from Merck</h4>Analytical Excellence Week' is organized to empower professionals in the field of analytical chemistry. Over the course of eight insightful sessions, industry experts will share their knowledge and best practices to streamline your analytical workflows and enhance your efficiency.From understanding quality grades in inorganics and solvents to exploring sustainable practices in the lab, each webinar covers essential topics that will enhance your analytical capabilities. Learn how to optimize your Karl Fischer titration, achieve precision in PFAS testing, and gain troubleshooting tips for LC-MS to effectively address common challenges and improve your laboratory results.The sessions will also address critical aspects of safety and sustainability, ensuring you are well-equipped to handle hazardous chemicals responsibly while making strides toward greener laboratory practices.This is an invaluable opportunity to gain insights that can significantly optimize your analytical processes. Don’t miss your chance to elevate your expertise and contribute to a more efficient and sustainable future in analytical workflows.Each session is specifically designed to address essential topics that directly impact your daily practices, making it relevant for all professionals working in a chemical analysis laboratory.<h3>Day 1: Monday, 10 February</h3><h4>Navigating Quality Grades Part 1: Inorganics and Solvents for Classical Analysis and Research</h4>Join us for an insightful webinar focused on the critical process of selecting the right quality grade for inorganic reagents and solvents used both in analytical and research applications. We will provide an in-depth overview of various quality grades, including ACS grade and EMSURE®, and discuss their specific attributes, including purity, regulatory support, and packaging. Gain valuable insights into the importance of this choice and its impact on the quality of your lab processes and outcomes.By attending this webinar, you will:<ul><li>Understand the significance of choosing the right quality grade for your lab applications</li><li>Explore the available quality grades, including ACS grade and EMSURE®, and their specific attributes</li><li>Recognize the impact of quality grade selection</li><li>Learn best practices for selecting and utilizing the appropriate quality grade for several applications</li></ul>Who should attend:This webinar is designed for professionals involved in laboratory work who seek to enhance their understanding of quality grades for inorganic reagents and solvents. It is particularly relevant for lab technicians, researchers, and managers in analytical labs (like QC, environmental, etc.) method development and research laboratories that are looking to optimize their processes and outcomes.<blockquote>Presenter: Christian Roth (Senior Product Manager Inorganics, Merck)Presenter: Lyra Christianson (Product Manager Solvents, Milwaukee, Wisconsin, USA, an affiliate of Merck)</blockquote><h4>Navigating Quality Grades Part 2: Solvents and Reagents for Chromatography Applications</h4>This second part focuses on the critical process of selecting the right quality grades used in chromatography applications. We provide an in-depth overview of various quality grades, from Suprasolv® solvents for GC to LiChrosolv® solvents for HPLC, in combination with LiChropurTM reagents, and discuss their specific attributes, including purity, regulatory support, and packaging. The topic will be illustrated by typical HPLC and LC-MS applications.By attending this webinar, you will learn:<ul><li>Which grades are suitable for which chromatographic analysis</li><li>What are the specific attributes of the solvents and reagents</li><li>How to combine different quality grades meaningfully</li><li>How to identify and differentiate between various quality grades of reagents and solvents.</li><li>To understand the critical importance of selecting the appropriate quality grade for your specific analytical applications</li></ul>Who should attendLab technicians, lab managers, and quality control-related persons who need to understand how to select the different quality grades for HPLC and LC-MS applications.<blockquote>Presenter: Patric Klein, (Global Product Manager Organic and Inorganics Instrumental Analysis, Merck)Presenter: Fernando Luengo, (Global Product Manager HPLC Solvents, Milwaukee, Wisconsin, USA, an affiliate of Merck)Presenter: Daniel Weibel, (Global Product Manager Solvents, Merck)</blockquote>

Webinar Series & Expert Roundtable (Day 1)

Navigating Quality Grades Part 1: Inorganics and Solvents for Classical Analysis and Research and Part 2: Solvents and Reagents for Chromatography Applications

Laboratory balance cleaning is extremely important for accurate weighing results and operator safety. It can be challenging but also fast and easy if you know how to do it right.A clean analytical balance or precision balance is the first step toward safe and accurate weighing. Cleaning increases user safety reduces the risk of cross-contamination, and is the most important action to prolong instrument life. Join our free live webinar to find out more about the benefits of and solutions for cleaning your balance.Program:<ul><li>Why cleaning lab equipment is important for safety and weighing accuracy</li><li>The consequences of not cleaning your balances correctly</li><li>How often you should clean your balance</li><li>The detergents that are suitable for balance cleaning</li><li>The balance features that support fast and effortless cleaning</li></ul><blockquote>Presenter: Adriano Guiso (Trainer, METTLER TOLEDO)</blockquote>

Mettler Toledo: Laboratory Balance Cleaning

Laboratory Balance Cleaning

Join our free live webinar to find out more about the benefits of and solutions for cleaning your balance.

<div><div id="inner_content_body_text" data-content-id="394785">Sustainable lab practices are becoming an increasingly important factor to many lab managers and personnel. This webinar will discuss the improvements you could make to your lab’s sustainability goals with the introduction of on-site gas generation.Our expert speakers will explore how on-site gas generation for LC-MS and gas chromatography compares to traditional supplies like cylinders and dewars, illustrating the environmental benefits from gas deliveries to power consumption. &nbsp;<div>Attend this webinar to:</div><ul><li>Discover the best sustainability practices when supplying your LC-MS or GC with nitrogen or hydrogen</li><li>Learn about MyGreenLab’s ACT accreditation and how you can be sure your lab instruments are not negatively impacting the environment</li><li>Find out about the “hidden” impacts of gas generation on your lab’s sustainability efforts&nbsp;</li></ul><blockquote>Presenter: Bruce Peat (Head of Marketing at PEAK Scientific)A data-driven, level-headed, well-disciplined and enthusiastic marketing professional with over 8 years' experience primarily in Scientific and Manufacturing industries. A passion for marketing, understanding customer and consumer needs, with a focus on delivering a customer experience to match.&nbsp;</blockquote></div></div>

Technology Networks: Toward More Sustainable Labs: The Impact of Gas Supply

Toward More Sustainable Labs: The Impact of Gas Supply

This webinar will discuss the improvements you could make to your lab’s sustainability goals with the introduction of on-site gas generation.

The presentation explores the integration of proteomics into clinical practice, particularly in cancer research. It emphasizes the use of mass spectrometry-based proteomics for molecular profiling of clinical cancer cohorts, combining genetic and proteomic data to understand disease mechanisms and patient outcomes. The speaker discusses the challenges and successes in translating proteomics discoveries into clinical routines, highlighting the importance of extensive clinical data and longitudinal studies. Case studies demonstrate how proteomics can provide deeper insights into diseases like lung cancer and multiple myeloma, with examples of high-throughput assays achieving significant protein coverage. The presentation also covers the application of machine learning to enhance data analysis and the potential of proteomics to improve diagnostic precision and treatment efficacy. Overall, it underscores the transformative potential of proteomics in personalized medicine and clinical diagnostics.Learning points :<ul><li>Integration of Proteomics in Clinical Practice: Learn how mass spectrometry-based proteomics can be utilized for molecular profiling and improving patient outcomes in clinical settings.&nbsp;</li><li>Challenges and Solutions in Translating Discoveries: Understand the hurdles and strategies for effectively incorporating proteomics discoveries into routine clinical practice, supported by extensive clinical data and longitudinal studies.&nbsp;</li><li>Enhanced Data Analysis with Machine Learning: Discover the role of machine learning in analyzing proteomics data to boost diagnostic precision and treatment efficacy in personalized medicine.</li></ul>Who should attend?<ul><li>Cancer Researchers: Professionals focused on integrating genetic and proteomic data for deeper insights into cancer mechanisms and treatments.&nbsp;</li><li>Clinical Data Scientists: Individuals interested in applying machine learning to enhance data analysis and improve diagnostic precision.&nbsp;</li><li>Healthcare Practitioners: Those looking to adopt proteomics for personalized medicine and more effective clinical diagnostics.</li></ul><h5>Additional Webinars</h5>Take advantage of our growing library of convenient and educational recorded webinars and resources. <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/about-us/events/industrial/analytical-innovations-webinars.html?cid=E.24CMD.EL112.21227.01">Visit our webinars page</a><blockquote>Presenter:&nbsp; Philipp Mertins, PhD (Head of Proteomics Platform, Max Delbrück Center and Berlin Institute of Healt)Philipp Mertins earned his PhD at the Max Planck Institute in Munich, focusing on ​signal transduction and proteomics research. He then joined the Broad Institute of MIT ​and Harvard, where he worked with Dr. Steve Carr as a staff scientist and group leader, ​significantly contributing to the Clinical Proteomic Tumor Analysis Consortium ​(CPTAC). Since 2017, Dr. Mertins has been heading the Proteomics Platform at the ​Max Delbrück Center and the Berlin Institute of Health in Berlin, where he continues to ​advance proteomic technologies in clinical proteomics and translational research.​</blockquote>

Thermo Fisher Scientific: Navigating proteomics techniques for molecular profiling of clinical cancer cohorts

Navigating proteomics techniques for molecular profiling of clinical cancer cohorts

The presentation explores the integration of proteomics into clinical practice, particularly in cancer research. 

Proteomics Techniques for Cancer Cohort Molecular Profiling

Discover the new Dual Chromatogram Mode for dual tower samplers, enhanced User Account settings, updated control modules, Extensions' improvements, and more.Additionally, during the Q&amp;A session, you will gain valuable tips for using Clarity software more effectively and get answers to your questions. Don't miss this opportunity to elevate your Clarity experience!The Webinar will be held on February 6th at 9:00 or 15:00 CET.

Clarity 10 Webinar

Discover the new Dual Chromatogram Mode for dual tower samplers, enhanced User Account settings, updated control modules, Extensions' improvements, and more.

Biotherapeutics offer targeted therapies for treating a wide range of diseases with greater specificity and efficacy. However, unlike traditional small-molecule drugs, biotherapeutics are typically larger and more complex, requiring advanced techniques for their production, purification, and characterization.Therapeutic proteins, first introduced many years ago, have continually advanced in complexity. Since the introduction of monoclonal antibodies, we now have antibody drug conjugates, bispecific antibodies, fragments and fusion proteins, all of which are in various stages of development.To keep pace with biotherapeutics advances, analytical techniques must also advance.In this webinar, we will present chromatography findings on:<ul><li>New workflows for intact and peptide mapping characterization of proteins</li><li>Advanced column chemistries for charge variant analysis with pH and salt gradients</li><li>Rapid desalting SEC column for high throughput analysis, ADC analysis with HIC and reversed phase chemistries</li><li>New digestion workflows coupled to a new peptide mapping column for sequence verification and post translation modification analysis</li></ul>This live webinar will be an excellent opportunity to boost your R&amp;D capabilities and strengthen your QC analytical methods for the characterization of biotherapeutics and gene therapies. Dr. Ken Cook will be presenting and answering your questions live.With over 20 years of innovation, Thermo Fisher Scientific is a leader in biomolecule separation and characterization, supported by a wealth of published research.<h5>Who should attend?</h5><ul><li>Pharmaceutical and Biotech Researchers: Researchers involved in the development and characterization of biotherapeutics and gene therapies, including those working with therapeutic proteins.</li><li>Quality Control (QC) Analysts: Analysts responsible for ensuring the quality and consistency of biotherapeutic products, who will benefit from advanced methods in characterization and validation.</li><li>R&amp;D Managers: Managers who oversee research and development teams working with biotherapeutics, looking to enhance their workflows and methodologies.</li></ul><h5>Additional Webinars</h5>Take advantage of our growing library of convenient and educational recorded webinars and resources. <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/about-us/events/industrial/analytical-innovations-webinars.html?cid=E.24CMD.EL112.21227.01">Visit our webinars page</a><blockquote>Presenter: Dr. Ken Cook (Applications Scientists Manager, Thermo Fisher Scientific)<div>Moved to industry from a University lectureship in Biochemistry, University of Newcastle upon Tyne, following 7 years’ experience in academia. Now with over 35 years’ experience with Thermo Fisher Scientific using a wide range of analytical equipment and software. Publications range from metabolomics, proteomics to large biopharmaceutical drug analysis. Current job title is the European manager for the BioPharma expert support group. This involves the support of Bio-Pharmaceutical applications to characterise protein and oligonucleotide based bio-therapeutics. This involves ongoing collaborations with the Biopharmaceutical industry and academia. Characterization methods of Bio-therapeutics include, monoclonal antibodies, peptides, AAV analytical development, small and large RNA analysis including sequence mapping of mRNA by LC/HRMS.</div></blockquote>

Thermo Fisher Scientific: Innovations in protein characterization

Innovations in protein characterization

The webinar will be excellent opportunity to boost your R&D capabilities and strengthen your QC analytical methods for biotherapeutics and gene therapies. 

<div><div><h3>Oligos, Oligos, Oligos!</h3>Oligonucleotide therapeutics and reagents are everywhere. They are helping the world address previously untreatable diseases, and they are also being used in new ways to sensitively quantify and sequence important translational research samples. Whether it be a CRISPR guide RNA, lipid conjugated anti-sense oligonucleotide, or a specially modified assay probe, these molecules must be tested to ensure they have the right sequence, pinpoint-specific modifications, conjugate moieties and purities. &nbsp;And when dosed as a therapeutic, they need to be carefully quantified to confirm pharmacokinetic models. &nbsp;Join the Waters Chromatography and LC-MS Team and their collaborators from MRIGlobal, Regeneron, and Acuitas Therapeutics in this special episodic, 3 week webinar series to learn more about the science of oligos and the power of LC-based approaches to analyze them.&nbsp;<h3>EPISODE 1</h3><h4>Part 1: Ultrafast IP-RP using ultra-short columns and new HILIC insights with GTxResolve Premier BEH Amide Columns</h4>In the first part of the presentation, we provide a comprehensive guide to the analysis of oligonucleotides (ONs) using ion-pair reversed-phase liquid chromatography (IP-RPLC) on ultra-short columns (2.1 x 20 mm). We investigate the effect of ion-pairing (IP) agents on ON retention and show that while IP agents significantly affect absolute retention, their effect on selectivity is often minimal. Our results underscore the utility of systematic method development, particularly to investigate gradient steepness and temperature - which have the greatest impact on selectivity - so that resolution can be optimized for both sequence and length variants. We discuss the importance of using low-adsorption column hardware to minimize non-specific interactions, which will improve method robustness and peak shapes. The webinar will provide practical guidelines for the development of robust, reproducible and highly efficient IP-RPLC methods for ONs, which will be useful for industry analysts working on new therapeutics as well as new sequencing and diagnostic reagents.The second part of the presentation will focus on some new insights into hydrophilic interaction chromatography (HILIC). Indeed, HILIC seems to be a very promising alternative to IP-RPLC. A new column (GTxResolve Premier BEH Amide) will be presented, which allows the development of fast and robust methods. Method considerations (selection of mobile phase additives, gradient optimization) will be discussed in detail. Solutions to avoid the inherent HILIC breakthrough effects (related to solvent/eluent mismatch) will be provided.Finally, generic (platform) conditions that can be set for the separation of different oligonucleotides will be discussed.<blockquote>Presenter: Szabolcs Fekete PhD (Consulting Scientist, Cell and Gene Therapy Consumables, Waters Corporation)Szabolcs worked in the pharmaceutical industry for 10 years before joining the University of Geneva Department of Pharmaceutical Sciences. In 2021, he joined Waters Corporation and now works as a Consulting Scientist. His current interests include separations of new chemical modalities, fundamentals of chromatography, column technology, and method development approaches. His current interests include separations of new chemical modalities, fundamentals of chromatography, column technology, new method development approaches and modeling. He was the winner of the LCGC Emerging Leader in Chromatography Award in 2020 and the HTC Innovation Award in 2022.</blockquote><h4>Part 2: Characterizing CRISPR-Cas9 Systems: Analyzing CRISPR Components using Ion Exchange and High Throughput IP-RP LC-MS</h4>Learn about leveraging the differing charges of apo-Cas, unbound sgRNA, and ribonucleoprotein (RNP) complexes in order to separate CRISPR components by ion exchange (IEX) high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection. We will discuss scaling traditional UPLC methods to achieve even higher throughput IP-RP LC-MS analyses. &nbsp;A workflow was developed to transfer a UPLC purity method for sgRNA from a 100 mm IP-RP column to a 20 mm high throughput column, which has reduced total method times from 30 to 5 min.<blockquote>Presenter: Rachel Ginther (Senior Manager Biologics &amp; Cell and Gene Therapy, MRI Global)Rachel obtained her PhD in Analytical Chemistry from the University of Kansas and has worked in the pharmaceutical industry for seven years. She manages programs in biologics and cell and gene therapy analysis and manufacturing and is actively leading the expansion within the organization into biologics.</blockquote></div></div>

Oligo LC-Based Characterization

Join Waters Chromatography, LC-MS Team & collaborators for a 3-week webinar series on oligos science & LC-based analysis.

With laboratories worldwide facing growing demands for accurate and accessible analytics, LabSolutions BiX 3.2 enables an updated platform to enhance instrument management and operator management while continuously improving efficiency.Join us for an exclusive session on Digital Classrooms, where we uncover the newest version of LabSolutions BiX – a state-of-the-art platform designed for laboratory analytics and asset management.Discover how these newly added features can help you evaluate laboratory efficiency at a glance, effectively schedule and monitor instrument analysis, and simplify the monitoring of lab performance, delivering insights to both instruments and operators.Don't miss this opportunity to unlock the full potential of your laboratory!<h5>Learning Objectives:</h5>Learn how LabSolutions BiX 3.2 helps you to:<ul><li>To effectively manage your laboratory</li><li>Efficiently plan your instrument analyses using the centralized instrument booking feature</li><li>Evaluate the percentage efficiency of instrument usage across the lab</li><li>Significantly enhance overall lab efficiency</li></ul><blockquote>Presenter: Kanimozhi Sivarajan (Informatics Manager Shimadzu (Asia Pacific) Pte Ltd)Dr. Kani is having 10 years of experince in Laboratory Management and Informatics &nbsp;Systems. She is serving as Informatics Manager at Shimadzu Asia Pacific, Singapore, &nbsp;providing Lab Informatics Solutions such as LabSolutions CS, LIMS and other QMS applications. She is supporting customers from various segments on implementing laboratory automation and responsible for providing consultation on streamlining Lab Workflows, Regulatory Compliance requirements for Electronic Data Management and Lab Practices/Analysis.&nbsp;</blockquote>

Shimadzu:  With The Latest LabSolutions BiX 3.2

With The Latest LabSolutions BiX 3.2

Join us for an exclusive session on Digital Classrooms, where we uncover the newest version of LabSolutions BiX 