<p><strong>What if you could assess a tumor's metastatic potential — without extensive sample prep — directly from tissue? Rachel Wood is working on exactly that.</strong></p><p><strong>Rachel Wood</strong> is a PhD candidate at Queen's University (Kingston, Ontario),&nbsp;working jointly in the labs of Dr. Richard Oleschuk (Chemistry) and Dr. Chris&nbsp;Nicoll (Pathology &amp; Molecular Medicine). Her research applies Ambient Ionization Mass Spectrometry — specifically the Liquid Microjunction Surface Sampling Probe &nbsp;(LMJ-SSP) and Desorption Electrospray Ionization (DESI) — to profile the metabolomics of non-metastatic and metastatic clear cell Renal Cell Carcinoma (ccRCC) cell lines. The long-term goal: a rapid, minimally invasive MS-based prognostic tool for kidney cancer.</p><p>This is the first reported use of LMJ-SSP to assess metastatic status in ccRCC —&nbsp;and early PCA results are already showing distinct separation between cell line&nbsp;<br>profiles.</p><h5><strong>In this episode, David and Rachel cover:</strong></h5><ul><li>Why <strong>ccRCC</strong> is such a resistant biomarker problem despite active genomic, proteomic, and metabolomic research</li><li>The clinical stakes of "<strong>diagnosed by accident</strong>" — and what a prognostic biomarker actually needs to do for a clinician</li><li>How AIMS complements (not replaces) LC-MS in a discovery-phase cancer workflow</li><li><strong>LMJ-SSP vs. DESI</strong>: what each technique sees that the other misses, including spatial resolution differences (~1mm vs. ~50µm)</li><li>Why Rachel runs LMJ-SSP on both a triple quad and a Q-TOF — and what each instrument contributes to the same research question</li><li>How her lab repurposed a 3D printer as a custom automated XYZ sampling stage with 50µm movement precision</li><li>Managing high-dimensional metabolomics data across two polarities, two cell lines, and two mass analyzers — the pipeline explained</li><li>What unsupervised machine learning (PCA) showed in early results</li><li>The path from cell lines to excised tumor tissue to, ultimately, liquid biopsies</li><li>Advice for analytical chemists considering work at the chemistry–oncology interface</li></ul><h3><strong>Video Transcription</strong></h3><p><strong>Kidney cancer remains a difficult biomarker problem, particularly when researchers try to distinguish tumors that are likely to behave aggressively from those that are not. In a recent episode of </strong><i><strong>Concentrating on Chromatography</strong></i><strong>, PhD researcher Rachel Wood discussed how she is combining rapid ambient ionization mass spectrometry, LMJ-SSP, DESI, multivariate data analysis, and conventional LC-MS workflows to investigate the molecular differences associated with clear cell renal cell carcinoma and its metastatic potential.</strong></p><h4><strong>Returning to the research question that started it all</strong></h4><p>Wood first encountered kidney cancer research during her undergraduate studies. Although she later moved into a more instrumentation-focused master’s project involving breast cancer, the subject of kidney cancer remained unfinished business.</p><p>When the opportunity arose to choose a PhD project, she returned to the field that had originally sparked her interest in research. She is now conducting her doctoral work at <strong>Queen’s University in Kingston, Ontario</strong>, in an interdisciplinary environment spanning chemistry, pathology, molecular medicine, computer science, and clinical research.</p><h4><strong>Why clear cell renal cell carcinoma is such a difficult biomarker problem</strong></h4><p>Wood’s research focuses on <strong>clear cell renal cell carcinoma (ccRCC)</strong>, which represents the majority of kidney cancer cases discussed in the interview.</p><p>The difficulty is not a lack of research effort. Genomics, proteomics, and metabolomics studies have all produced promising biomarker candidates. The challenge is that different studies often identify different sets of markers, with relatively little overlap between them.</p><p>Patient-to-patient biological variability further complicates the picture. A candidate biomarker panel that performs well in one cohort may not reproduce equally well in another. Selecting which features deserve further investigation therefore becomes a major analytical and biological challenge.</p><h4><strong>From incidental diagnosis to prognostic information</strong></h4><p>Another difficulty is the way kidney cancer is often discovered.</p><p>Rather than being detected through a dedicated screening program, tumors may be found incidentally during imaging performed for another reason. According to Wood, the ideal solution would be a diagnostic biomarker capable of detecting kidney cancer early. Such a marker is not currently available within the scope of the work she described.</p><p>That places additional importance on <strong>prognostic biomarkers</strong>. Once a tumor has been detected, clinicians need as much information as possible about how aggressive it may be and how likely it is to progress.</p><p>Wood’s current work is therefore not attempting to declare directly that a particular patient has metastatic disease. Instead, the goal is to understand <strong>what changes biologically as a tumor moves from a less aggressive to a more aggressive state</strong>.</p><p>That means asking which molecular pathways change, which mechanisms become altered, and which analytes may eventually provide useful indicators of tumor behavior.</p><h4><strong>AIMS and LC-MS answer different questions</strong></h4><p>A central theme of the interview was the relationship between rapid ambient ionization mass spectrometry and conventional LC-MS.</p><p>Wood is clear that <strong>AIMS is not intended to replace LC-MS</strong>. Instead, she sees the two approaches as complementary.</p><p>AIMS provides a rapid way to obtain a broad molecular overview of a sample with minimal preparation. This makes it especially useful during the discovery phase, when the goal is to gather as much molecular information as possible and identify features that distinguish biological groups.</p><p>LC-MS then becomes valuable when those features need to be separated, characterized more carefully, and quantified with greater confidence.</p><p>In Wood’s workflow, rapid MS screening can therefore help identify which analytes deserve closer attention before more targeted LC-MS investigations are performed.</p><h4><strong>What “minimal sample preparation” actually means</strong></h4><p>The amount of sample preparation required depends strongly on the type of material being analyzed.</p><p>Wood currently works mainly with <strong>cultured human cell lines</strong>, which means much of the preparation begins with growing the cells themselves. Depending on the experiment, the cells may then be chemically lysed or subjected to fixation, paraffin embedding, and sectioning before analysis by <strong>LMJ-SSP</strong> or <strong>DESI</strong>.</p><p>She also notes that the possibilities would be different for freshly excised tumor material. In an appropriate perioperative or biosafety laboratory setting, LMJ-SSP could potentially be used to analyze tumor margins more directly.</p><p>The practical meaning of “minimal preparation” is therefore highly dependent on the sample and the analytical question being asked.</p><h4><strong>Why combine LMJ-SSP and DESI?</strong></h4><p>Rather than choosing between LMJ-SSP and DESI, Wood uses the two approaches because they provide different types of information.</p><p><strong>LMJ-SSP</strong> offers rapid, localized analysis and can accommodate samples with uneven surface topography, which is particularly useful for excised tissue.</p><p><strong>DESI</strong>, by contrast, provides substantially higher spatial resolution. In the workflow described in the interview, DESI can achieve spatial resolution on the order of tens of micrometers, whereas LMJ-SSP operates at a much larger sampling scale depending on probe design.</p><p>That higher spatial resolution is important when moving from simplified cell-line models toward actual tumor tissue, where the molecular organization of different regions may reveal biologically relevant differences.</p><p>Combining both techniques therefore enables researchers to balance speed, surface compatibility, and spatial information.</p><h4><strong>Two mass analyzers for two parts of the same problem</strong></h4><p>Wood also uses two different mass spectrometers in her LMJ-SSP work.</p><p>Most of the discovery work is carried out using a <strong>triple quadrupole mass spectrometer</strong>, particularly because her project focuses strongly on lower-mass analytes and small molecules.</p><p>Once statistically important analytes have been identified, a <strong>Q-TOF</strong> instrument can be used for MS/MS experiments and tentative structural identification.</p><p>The two instruments therefore address different stages of the workflow: one supports broad discovery and comparison, while the other provides additional structural information needed to connect selected features with possible metabolic pathways.</p><h4><strong>Turning a 3D printer into an automated sampling platform</strong></h4><p>One of the most unusual elements of the project is the automated sampling system used with LMJ-SSP.</p><p>Historically, LMJ-SSP measurements could be performed manually or with custom-built XYZ positioning stages. Wood’s laboratory instead adapted a <strong>3D printer</strong> into an automated positioning system.</p><p>Custom software controls the printer and moves the sampling probe with precise control over its X, Y, and Z positions. According to Wood, the system enables movements down to approximately <strong>50 µm</strong> and also controls parameters such as the contact time between the probe and sample and the delay between consecutive sampling events.</p><p>This level of automation improves reproducibility because variables that would otherwise depend on manual operation can be controlled systematically.</p><p>Even the Z-position can be programmed, allowing the probe to operate at constant or varying heights depending on the sample surface.</p><h4><strong>Managing high-dimensional MS data</strong></h4><p>The experimental design creates a large data-analysis challenge.</p><p>Wood is working with multiple cell lines, both ionization polarities, and more than one mass analyzer. To maintain traceability, she analyzes the data in clearly defined subsets.</p><p>A typical workflow might begin with one mass analyzer and one polarity. Within that condition, the lower-aggressiveness and higher-aggressiveness cell models can then be compared directly.</p><p>The data processing pipeline includes steps such as:</p><ul><li>binning,</li><li>normalization,</li><li>transformation,</li><li>dimensionality reduction,</li><li>identification of discriminating molecular features.</li></ul><p>Once relevant features are identified, MS/MS analysis can be used to investigate them further. The same process is then repeated across other polarities and instruments.</p><h4><strong>Unsupervised machine learning reveals distinct molecular profiles</strong></h4><p>Machine learning is particularly valuable because the AIMS datasets are highly dimensional, containing very large numbers of molecular features across multiple samples.</p><p>Wood’s initial analysis has included <strong>principal component analysis (PCA)</strong>, an unsupervised dimensionality-reduction technique.</p><p>The early results are encouraging: the non-metastatic and metastatic cell-line models separate clearly in PCA space.</p><p>That does not itself identify a definitive biomarker panel, but it indicates that the two biological states have distinguishable molecular profiles even though both belong to the same kidney cancer subtype.</p><p>This provides an important foundation for identifying which specific metabolites or molecular pathways may be responsible for the separation.</p><h4><strong>Testing LMJ-SSP for metastatic status</strong></h4><p>A particularly novel aspect of Wood’s project is the use of LMJ-SSP to investigate differences associated with metastatic status.</p><p>She had previously worked on distinguishing healthy and cancerous tissues, where the biological differences are relatively large. Comparing two states within the same cancer type is more demanding because the molecular changes may be much subtler.</p><p>The scientific motivation, however, is strong. Tumors discovered at later stages carry greater concern for metastatic progression, making it important to understand the molecular changes associated with increasingly aggressive behavior.</p><p>For Wood, this made the methodological risk worthwhile: the analytical challenge directly addresses an area where better prognostic information could ultimately have clinical value.</p><h4><strong>A long-term goal: rapid MS-based prognostic testing</strong></h4><p>The long-term vision extends beyond cell-line research.</p><p>Wood is interested in whether rapid, minimally invasive mass spectrometry could eventually contribute to clinical decision-making.</p><p>One possibility would be identifying a diagnostic biomarker in a <strong>liquid biopsy</strong>, potentially using blood or urine. A urine-based marker capable of contributing to earlier detection would be particularly attractive for kidney cancer.</p><p>For prognostic applications, the goal would be slightly different. Once a tumor is discovered, molecular information could help characterize its likely behavior and provide clinicians with additional evidence when deciding between immediate treatment, surgery, or other management strategies.</p><p>Wood emphasizes that such a tool would not replace standard clinical workflows. Instead, it would provide additional molecular information to complement existing diagnostic and pathological approaches.</p><h4><strong>Why interdisciplinary work matters</strong></h4><p>Throughout the interview, Wood repeatedly returns to the importance of collaboration.</p><p>Her research sits at the intersection of analytical chemistry, pathology, oncology, computation, and clinical medicine. No single researcher can remain equally current in all of these fields.</p><p>Working closely with pathologists, computer scientists, surgeons, medical students, and other specialists therefore provides both scientific expertise and a way to stay connected with developments outside one’s immediate discipline.</p><p>For Wood, the biological context has also changed how she thinks about analytical chemistry. Developing a new instrument or method is important, but understanding <strong>who may ultimately benefit from that analytical capability</strong> makes the research more meaningful.</p><p>That patient-centered perspective is now an integral part of how she approaches the project.</p><h4><strong>An intersection full of opportunities</strong></h4><p>Wood describes the interface between analytical chemistry and oncology as an especially exciting area for young researchers.</p><p>The work can range from troubleshooting instrumentation to interpreting pathology, developing statistical methods, and collaborating across disciplines. That diversity creates constant opportunities to learn and to apply analytical chemistry to clinically relevant questions.</p><p>Her project illustrates that modern mass spectrometry research is increasingly about more than generating spectra. It involves connecting instrumentation, spatial sampling, multivariate statistics, machine learning, biological interpretation, and clinical needs into a single analytical strategy.</p><p>And in kidney cancer, where reliable prognostic biomarkers remain difficult to establish, that integrated approach may be particularly valuable.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

AIMS vs. LC-MS for Cancer Diagnostics: ccRCC Metabolomics with Rachel Wood

<p><strong>In the week of September 21, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>6 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Waters Corporation: Strengthening SPE Workflows for Today’s Laboratory</strong></h5><ul><li>Mo, 21.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6514">Registration</a></li></ul><p>Learn how to optimize SPE workflows with best practices for sorbent selection, recovery troubleshooting, and reducing matrix effects in modern analytical laboratories.</p><h5><strong>2. Agilent Technologies: From Crude to Candidate: Scaling Preparative Chromatography with Confidence</strong></h5><ul><li>Mo, 21.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6498">Registration</a></li></ul><p>Learn how to scale preparative chromatography from crude sample to purified candidate, with practical strategies for method development, fraction collection, and method transfer.</p><h5><strong>3. SCIEX: Extending the power of LC MS/MS: advanced workflows with QTRAP®</strong></h5><ul><li>Tu, 22.9.2026 14:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6518">Registration</a></li></ul><p>Discover how MRM³ and QTRAP workflows provide greater selectivity and lower limits of quantification for LC-MS/MS analysis in complex matrices.</p><h5><strong>4. Technology Networks: Improving Efficiency in Routine PFAS Analysis Using EPA Methods 533 and 1633A</strong></h5><ul><li>Tu, 22.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6517">Registration</a></li></ul><p>Explore how automated QC monitoring and the SCIEX novus V55 system support high-throughput PFAS quantitation using EPA Methods 533 and 1633A.</p><h5><strong>5. Phenomenex: Expanding the Evidence: New Data for More Reliable PFAS Analysis</strong></h5><ul><li>We, 23.9.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6522">Registration</a></li></ul><p>Discover how new analytical data and contamination control strategies can improve baseline performance, sensitivity, and reliability in PFAS analysis.</p><h5><strong>6. SCIEX: How is capillary electrophoresis being used to analyze DNA and RNA</strong></h5><ul><li>We, 23.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6521">Registration</a></li></ul><p>Explore how capillary electrophoresis supports DNA and RNA analysis, from plasmid quality assessment to mRNA impurity profiling, viral vector characterization, and oligonucleotide workflows.</p><h5><strong>7. Thermo Fisher Scientific: A novel monodisperse supermacroporous reversed-phase platform for comprehensive nucleic acid analysis across a broad size range</strong></h5><ul><li>We, 23.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6476">Registration</a></li></ul><p>Discover how a novel monodisperse supermacroporous reversed-phase platform enables comprehensive nucleic acid analysis across a broad size range, from short oligonucleotides to mRNA.</p><h5><strong>8. Waters Corporation: The Next Frontier in Nitrosamines Testing: Detection and Risk Assessment in Packaging and IV Infusion Bags</strong></h5><ul><li>We, 23.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6520">Registration</a></li></ul><p>Learn how LC-MS/MS supports nitrosamine detection and risk assessment in packaging materials while addressing extractables and leachables challenges in IV infusion bags.</p><h5><strong>9. MDPI: Membrane Technologies for Water and Food Sustainability</strong></h5><ul><li>We, 23.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6561">Registration</a></li></ul><p>Explore membrane technologies for water and food sustainability, international research collaboration, and opportunities within the CYTED Program.</p><h5><strong>10. Agilent Technologies: Unlocking Lab Productivity: Automation Opportunities for LC/MS Laboratories</strong></h5><ul><li>We, 23.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6495">Registration</a></li></ul><p>Discover how automation can increase productivity in LC/MS laboratories by reducing manual tasks, improving workflow efficiency, and supporting scalable analytical operations.</p><h5><strong>11. Thermo Fisher Scientific: Comparison of biocompatible and inert UHPLC systems for LC-UV quantitation of large RNA</strong></h5><ul><li>Th, 24.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6477">Registration</a></li></ul><p>Learn how the Vanquish Amplify system minimizes metal interactions during LC-UV analysis of large RNA and explore the performance of biocompatible and inert UHPLC systems.</p><h5><strong>12. Agilent Technologies: Next-Generation Pathogen Genomics: The Long-Read Advantage</strong></h5><ul><li>Th, 24.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6526">Registration</a></li></ul><p>Explore long-read sequencing and automated target enrichment in pathogen genomics and learn how Agilent SureSelect and Magnis NGS support improved pathogen detection.</p><h5><strong>13. Evosep Biosystems: Advancing Plasma Proteomics</strong></h5><ul><li>Th, 24.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6523">Registration</a></li></ul><p>Discover advances in plasma proteomics and learn how high-throughput analytical workflows can accelerate biomarker discovery and cancer research.</p><h5><strong>14. Waters Corporation: Applications of Multi-Angle Light Scattering for Biotherapeutic Characterization</strong></h5><ul><li>Th, 24.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6430">Registration</a></li></ul><p>Learn how multi-angle light scattering (MALS) supports confident characterization of monoclonal antibodies, antibody-drug conjugates, and AAV viral vectors.</p>

Webinars LabRulezLCMS Week 39/2026

Forced degradation analysis of recombinant adeno-associated virus serotype 8 based on analytical anion exchange chromatography coupled to orthogonal characterization

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Mestrelab Mnova Gears - Chrom Cal software

Chrom Cal is an advanced software solution developed by Mestrelab Research to automate and streamline the creation of calibration curves for accurate chromatographic quantitation

<h4><strong>Automated evaluation and generation of calibration curves for multiple compounds</strong></h4><p data-start="0" data-end="639"><strong data-start="0" data-end="13">Chrom Cal</strong> is an advanced software solution developed by Mestrelab Research to <strong>automate</strong> and <strong>streamline the creation of calibration curves for accurate chromatographic quantitation</strong>. Fully integrated within the Mnova suite, Chrom Cal efficiently processes spectral datasets, plots integration areas against known analyte concentrations, performs statistical analyses to fit calibration lines, validates results based on pre-set criteria, and generates automatic reports and result files for downstream quantitation. This comprehensive approach enhances both efficiency and accuracy, effectively handling multiple compounds simultaneously.</p><h4><strong data-start="641" data-end="654">Benefits:</strong></h4><ul><li><strong>Easy generation of calibration curves </strong>for all the compounds in one run.</li><li><strong>Huge time saving</strong> <strong>on result reviewing</strong>, enabling a review-by-exception strategy.</li><li><strong>Better understanding and revision of the results</strong> taking into account the spectral context.</li><li><strong>Seamless transfer of calibration results to downstream analyses</strong>, avoiding transcription errors.</li></ul><h4><strong>Features:</strong></h4><h5><strong data-start="1039" data-end="1108">Automatic pick-up and grouping of spectral datasets for analysis</strong></h5><p data-start="1039" data-end="1542">Chrom Cal is fully integrated in Mnova and can handle spectral datasets, extracting the information needed for calculating calibration curves without the need for data transcription into third-party software or spreadsheets. After data ingestion, Chrom Cal can detect and group samples from different compounds automatically based on an input CSV file, allowing calibration curves for those compounds to be generated in a single run.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mestrelab_Mnova_Gears_Chrom_Cal_Automatic_pick_up_and_grouping_of_spectral_datasets_for_analysis_f4dab8cce6.webp" alt="Mestrelab Research: Mestrelab Mnova Gears - Chrom Cal - Automatic pick-up and grouping of spectral datasets for analysis" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mestrelab_Mnova_Gears_Chrom_Cal_Automatic_pick_up_and_grouping_of_spectral_datasets_for_analysis_f4dab8cce6.webp 182w," sizes="100vw" width="1282" height="1100"></p><h5><strong data-start="1546" data-end="1659">Powerful algorithms to fit calibration curves and evaluate results based on pre-defined quality requirements</strong></h5><p data-start="1546" data-end="2109">Chrom Cal processes spectral data, integrates compound peaks, and fits calibration curves to data points in a fully automated fashion. Acceptance criteria, such as R² value thresholds or slope ranges, and cross-validation rules can be pre-defined in the method and evaluated at the end of the analysis to approve or reject calibration results. This allows control over the quality and linearity of results and speeds up the result reviewing process.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mestrelab_Mnova_Gears_Chrom_Cal_Powerful_algorithms_to_fit_calibration_curves_and_evaluate_results_based_on_pre_defined_quality_requirements_65569ca27a.webp" alt="Mestrelab Research: Mestrelab Mnova Gears - Chrom Cal - Powerful algorithms to fit calibration curves and evaluate results based on pre-defined quality requirements" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mestrelab_Mnova_Gears_Chrom_Cal_Powerful_algorithms_to_fit_calibration_curves_and_evaluate_results_based_on_pre_defined_quality_requirements_65569ca27a.webp 245w," sizes="100vw" width="1448" height="910"></p><h5><strong data-start="2113" data-end="2150">Live results in tables and plots</strong></h5><p data-start="2113" data-end="2622">Calibration and validation results are displayed in a result viewer that allows both global and detailed review of the data. Users can navigate between compounds to check standard curve plots and parameters. Each data point on the graph is dynamically linked to results tables and spectra. Users can zoom in or out on the plot, include or exclude samples from the analysis, or correct peak assignments or integrations when needed, with results automatically recalculated.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mestrelab_Mnova_Gears_Chrom_Cal_Live_results_in_tables_and_plots_a3b64eaabb.webp" alt="Mestrelab Research: Mestrelab Mnova Gears - Chrom Cal - Live results in tables and plots" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mestrelab_Mnova_Gears_Chrom_Cal_Live_results_in_tables_and_plots_a3b64eaabb.webp 245w," sizes="100vw" width="1328" height="827"></p><h5><strong data-start="2626" data-end="2687">Multiple analysis outputs nicely generated and organized</strong></h5><p data-start="2626" data-end="3043">Chrom Cal generates automatic CSV, Mnova, PDF, and HTML reports with calibration and validation results, saved in chosen locations along with metadata, logs, and settings, improving data management. Calibration results in JSON files feed downstream quantitative analysis, allowing extraction without manual transcription, reducing errors, and saving time.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mestrelab_Mnova_Gears_Chrom_Cal_Multiple_analysis_outputs_nicely_generated_and_organized_86d47119e2.webp" alt="Mestrelab Research: Mestrelab Mnova Gears - Chrom Cal - Multiple analysis outputs nicely generated and organized" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mestrelab_Mnova_Gears_Chrom_Cal_Multiple_analysis_outputs_nicely_generated_and_organized_86d47119e2.webp 245w," sizes="100vw" width="1153" height="709"></p>

Mestrelab Mbook 4.0 

Mbook, a powerful Electronic Laboratory Notebook (ELN), is designed specifically for chemists, providing a streamlined solution for managing experimental data.

<p class="cke-user_style-important"><strong>Designed by chemists, for chemists</strong></p><p>In today's chemistry labs, efficiency, data security, and seamless collaboration are essential for success. <a target="_blank" rel="noopener noreferrer" href="https://mestrelab.com/main-product/mbook-chemistry?utm_source=adv&amp;utm_medium=labrulez&amp;utm_campaign=mar&amp;utm_term=mbook&amp;utm_content=blogpost"><strong>Mbook</strong></a>, a powerful Electronic Laboratory Notebook (ELN), is designed specifically for chemists, providing a streamlined solution for managing experimental data, analytical results, team coordination, stockroom inventory, and overall workflow efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mbook_Header_Image_e5fa76cd18.jpg" alt="Mestrelab Research: Mbook 4.0" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mbook_Header_Image_e5fa76cd18.jpg 242w," sizes="100vw" width="1406" height="906"></p><h4><strong>Why choose Mbook?</strong></h4><p>Mbook 4.0 is more than just an ELN for recording your reactions, projects, or experiments—it offers advanced capabilities to optimize your laboratory management, including:</p><ul><li>Experiment witnessing for enhanced supervision and confidence</li><li>COSHH assessments to simplify chemical safety management</li><li>Comprehensive inventory management with a Compound DB of over 2,500 preloaded compounds</li><li>Customizable Stockroom with PDF exportable options</li><li>Bottle consumption and tracking for accurate inventory control</li><li>Customizable Reports for actionable insights</li><li>Integrated raw data processing and analysis powered by Mnova 15.1</li><li>In-app messaging system for seamless communication</li><li>Advanced experiment write-up section for detailed documentation<br>…and much more!</li></ul><h4><strong>What’s New in Mbook 4.0?</strong></h4><p>Mbook continues to evolve with cutting-edge features and improvements. <strong>Mbook 4.0 is now available</strong>, bringing enhanced usability, performance upgrades, and deeper integration with advanced analytical tools. Explore its powerful features today!<br>Start optimizing your lab workflow today! <a target="_blank" rel="noopener noreferrer" href="https://mestrelab.com/mbook-trial"><strong>Click here </strong></a>to begin your free trial of Mbook.</p>

Mestrelab Mnova Hub software

Mnova Hub is a data browser that connects to various data providers and allows you to seamlessly retrieve, modify, and store data from within Mnova.

<p><strong>Streamlining Data Access and Exploration through Seamless Integration</strong></p><p>Spectral data is often scattered across diverse file systems and cloud-based repositories and is difficult to access. Mnova Hub is a data browser that connects to various data providers and allows you to seamlessly retrieve, modify, and store data from within Mnova, offering a centralized platform for exploration and analysis.</p><h4><strong data-start="641" data-end="654">Benefits:</strong></h4><ul><li>Seamlessly push and pull data from various providers using our unique data browser tool.</li><li>Cut out the middleman! Forget about manually downloading data; open and view it directly within Mnova.</li><li>Do not compromise security for easy data browsing; enjoy the ‘best of both worlds’ by effortlessly navigating secure data platforms.</li></ul><h4><strong>Features:</strong></h4><h5><strong data-start="1039" data-end="1108">Connection to various data providers</strong></h5><p>Mnova Hub provides easy and safe connections to various data sources, including databases (SQL and MnDB), Scientific Data Management Systems and data hubs (LOGS, ZONTAL), TopSpin (part of the Bruker Chemist Suite), and file systems. Each provider has a unique setup dialog that allows for customized configuration. Its flexible design ensures that integrating new providers is easily achievable.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mestrelab_Mnova_Hub_Connection_to_various_data_providers_ecc1b930ce.webp" alt="Mestrelab Research: Mestrelab Mnova Hub - Connection to various data providers." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mestrelab_Mnova_Hub_Connection_to_various_data_providers_ecc1b930ce.webp 245w," sizes="100vw" width="1104" height="641"></p><h5><strong data-start="1546" data-end="1659">Data searches and filtering</strong></h5><p>Mnova Hub has a configurable results display table that adapts to your preferences, allowing you to preview data, sort and filter results using available metadata filters, and fine-tune your search. This enables you to quickly and efficiently identify relevant datasets.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mestrelab_Mnova_Hub_Data_searches_and_filtering_fd7c371f66.webp" alt="Mestrelab Research: Mestrelab Mnova Hub - Data searches and filtering" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mestrelab_Mnova_Hub_Data_searches_and_filtering_fd7c371f66.webp 245w," sizes="100vw" width="1196" height="694"></p><h5><strong data-start="2113" data-end="2150">Seamless Data Management with Cloud Advantages</strong></h5><p>Mnova Hub offers a seamless experience for opening and saving data with just one step, akin to managing files on a local file system. With Mnova Hub, you no longer need to compromise between the convenience of local file handling and the advanced security, accessibility, and searchability features of cloud storage.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mestrelab_Mnova_Hub_Seamless_Data_Management_with_Cloud_Advantages_8b8effce32.webp" alt="Mestrelab Research: Mestrelab Mnova Hub - Seamless Data Management with Cloud Advantages." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mestrelab_Mnova_Hub_Seamless_Data_Management_with_Cloud_Advantages_8b8effce32.webp 245w," sizes="100vw" width="1104" height="640"></p>

Mestrelab Mnova ElViS software

By integrating Mnova ElViS into your analytical workflows, you can enhance the efficiency and accuracy of your spectroscopic data analysis across a wide range of techniques.

<h4><strong>Visualize, process, analyze and report various electronic and vibrational spectroscopic techniques</strong></h4><p>Carry out the analysis of various optical spectroscopy data including ultraviolet and visible (UV/Vis), near and mid infrared (NIR/MIR), Raman, fluorescence, and other spectroscopic methods operating in the whole region of wavelength from 100 nm to 100 µm.</p><h4><strong data-start="641" data-end="654">Benefits:</strong></h4><ul><li>Spectroscopic processing and analysis of ultraviolet and visible (UV/Vis), near and mid infrared (NIR/MIR), Raman, fluorescence methods.</li><li>Vendor and Operating System agnostic, supports most data file formats.</li><li>Wide range of processing functions, including a dynamic processing template.</li><li>Manual and automatic peak picking analysis.</li><li>Data Analysis of stacked/arrayed spectra.&nbsp;</li></ul><h4><strong>Features:</strong></h4><h5><strong data-start="1039" data-end="1108">PLS-Based Baseline Correction Tool (IarPLS)</strong></h5><p>To improve the processing of electronic and vibrational spectroscopy data, Mnova 15 introduces an enhanced PLS-based baseline correction tool, IarPLS (penalized-least-squares based). It now features an automatic button to compute the near-optimal asymmetry parameter.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mnova_EI_Vis_PLS_Based_Baseline_Correction_Tool_Iar_PLS_f3180f6927.webp" alt="Mestrelab Research: Mnova EIVis - PLS-Based Baseline Correction Tool (IarPLS)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mnova_EI_Vis_PLS_Based_Baseline_Correction_Tool_Iar_PLS_f3180f6927.webp 245w," sizes="100vw" width="1500" height="845"></p><h5><strong>Normalization Processing Modes</strong></h5><p>Three Normalization Processing modes, namely Largest Peak, Integral and Vector Length are available in Mnova 15. These three features aim to simplify the baseline correction process, streamline your workflow, and provide a rapid method for normalizing data to address quantitative issues.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mnova_EI_Vis_Normalization_Processing_Modes_675cb7c407.webp" alt="Mestrelab Research: Mnova EIVis - Normalization Processing Modes" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mnova_EI_Vis_Normalization_Processing_Modes_675cb7c407.webp 245w," sizes="100vw" width="1500" height="844"></p><h5><strong>Electronic and Vibrational Spectroscopies supported file formats</strong></h5><p>Mnova ElViS supports the most frequently used data file formats for these analytical techniques, including import and export of single and multi-column matrix-like spectra (i.e., ASCII XY and XYY, as .txt or .csv files). Supported formats include SPC (Galactic), JCAMP-DX, OPUS (Bruker), SPA and SPG (Omnic), Perkin Elmer's .sp files, and ASCII (XY and XYY).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mnova_EI_Vis_Electronic_and_Vibrational_Spectroscopies_supported_file_formats_bb6afc3eab.webp" alt="Mestrelab Research: Mnova EIVis - Electronic and Vibrational Spectroscopies supported file formats" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mnova_EI_Vis_Electronic_and_Vibrational_Spectroscopies_supported_file_formats_bb6afc3eab.webp 245w," sizes="100vw" width="1500" height="910"></p><h5><strong>Manual &amp; automatic spectral analysis</strong></h5><p>Mnova ElViS provides both manual and automatic peak picking, offering flexibility and convenience in your spectral analysis. It also features advanced tools for the analysis of stacked or arrayed spectra, ensuring thorough data evaluation and interpretation for all your spectral needs.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mnova_EI_Vis_Manual_and_automatic_spectral_analysis_e24a19a151.webp" alt="Mestrelab Research: Mnova EIVis - Manual &amp; automatic spectral analysis" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mnova_EI_Vis_Manual_and_automatic_spectral_analysis_e24a19a151.webp 240w," sizes="100vw" width="940" height="611"></p><h5><strong>Main processing features</strong></h5><p>Mnova ElViS comes with a range of useful processing features, including Multiplicative Scatter Correction, Probabilistic Quotient Normalization, Baseline correction (both automatic IarPLS and tailored Multipoint Baseline Correction), several smoothing techniques (such as Savitzky-Golay, Nonlocal Means, Exponential), normalization and Standard Normal Variate (SNV), derivatives, and units conversion between absorbance and transmittance.<br><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mestrelab_Research_Mnova_EI_Vis_Main_processing_features_bc6eb64b99.webp" alt="Mestrelab Research: Mnova EIVis - Main processing features" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mestrelab_Research_Mnova_EI_Vis_Main_processing_features_bc6eb64b99.webp 245w," sizes="100vw" width="1074" height="581"></p>

Watrex Streamline™ P1 HPLC Pump, PEEK

The Watrex Streamline™ P1 pump is a versatile and powerful dual-piston HPLC pump.

<p><strong>Streamline P1 pump, PEEK fully meets the demanding requirements for HPLC pumps and can be combined seamlessly with HPLC detectors and instruments from other manufacturers.</strong></p><blockquote><p class="cke-user_style-important"><strong>Price 4 935 € ex VAT</strong></p></blockquote><p>In the analytical version of the pump, the flow rate range from 0.001 ml/min to 10 ml/min is adjustable in 1 ul increments with a maximum working pressure of 42 MPa (6,000 psi). Flow rate adjustment accuracy is ±1% and flow rate variation is better than ±0.1%. The pump’s extremely smooth flow profile is ensured by an efficient pulse damper and optimized cam shape.</p><p>A unique feature is the active piston and seal rinse, which prevents crystallization of buffers. A touch screen display on the front panel allows intuitive control of the pump.</p><p>The display shows the flow rate, the current pressure and the pump is equipped with pressure limits. A <strong>PRIME function</strong> is also directly available for flushing at increased flow rates.</p><figure class="image"><img style="aspect-ratio:1024/768;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Streamline_P1_Pump_PEEK_dbc4418900.jpg" alt="Watrex: Streamline™ P1 Pump, PEEK." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Streamline_P1_Pump_PEEK_dbc4418900.jpg 208w," sizes="100vw" width="1024" height="768"></figure><h3><strong>TECHNICAL SPECIFICATION</strong></h3><ul><li><strong>Flow Rates:</strong> 0.001 to 10.000 mL/min</li><li><strong>Pressure:</strong> 0 to 6,000 psi (42 MPa)</li><li><strong>Flow precision:</strong> &gt; 0.2% RSD</li><li><strong>Flow accuracy:</strong> ± 1 % @ 1mL/min and 7 MPa,</li><li><strong>Pressure accuracy:</strong> ± 2% of full-scale, with 80:20 H2O/IPA @ 7 MPa</li><li><strong>Pulsation:</strong> ± 0.5 % @ 1mL/min and 7 MPa</li><li><strong>Remote inputs:</strong> USB</li></ul>

Watrex Streamline™ PDA1 HPLC Detector, PEEK

Photo-diode-array (PDA) HPLC detector with the optimum wavelength can be selected for each analyzed substance.

<p><strong>Watrex Streamline™ PDA1 is a photo-diode-array (PDA) detector for routine analysis and sophisticated research.</strong></p><blockquote><p class="cke-user_style-important"><strong>Price 9 972 € ex VAT</strong></p></blockquote><p>The <strong>dual lamp</strong> design offers a wavelength range of <strong>190 – 720 nm</strong> (256 Diodes) with a low baseline noise.</p><p>The front-accessible flow cell can easily be exchanged, as can be the lamps which are accessible through a side panel in the instrument housing.</p><p>Streamline™ PDA1 features <strong>4-Wavelength channels</strong> to measure chromatograms at <strong>4 different wavelengths</strong> at the same time. With this feature the optimum wavelength can be selected <strong>for each analyzed substance.</strong></p><figure class="image"><img style="aspect-ratio:945/435;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_Streamline_PDA_1_Photodiode_array_detector_3784ec588b.jpg" alt="Watrex: Streamline™ PDA1 Detector, Stainless Steel" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Watrex_Streamline_PDA_1_Photodiode_array_detector_3784ec588b.jpg 245w," sizes="100vw" width="945" height="435"></figure><h3><strong>TECHNICAL SPECIFICATION</strong></h3><ul><li><strong>Wetted Materials:</strong> Stainless Steel/ PEEK*, teflon, Glass (*depending on configuration)</li><li><strong>Light Source:</strong> Deuterium Lamp, Tungsten Lamp</li><li><strong>Wavelength Range:</strong> 190 – 720 nm</li><li><strong>Number of Diodes:</strong> 256</li><li><strong>Baseline Noise:</strong> ± 1 x 10-5 AU (@240 nm, 1 sec. risetime)</li><li><strong>Baseline Drift:</strong> better than 3 x 10-4 AU/h</li><li><strong>Wavelength Accuracy:</strong> 0.5 nm</li><li><strong>Mean Pixel Pitch:</strong> 2.2 nm</li><li><strong>Resolution:</strong> 7 nm</li><li><strong>Linearity:</strong> higher than 2.0 AU</li><li><strong>Data Rate:</strong> 1 Hz – 100 Hz</li></ul><p><strong>You can also visit this product on the </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/detectors/uv-vis-detectors/streamline-pda1-detector-256-diodes-2/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Watrex website</strong></a><strong>.</strong></p>

Watrex Streamline™ UV1 HPLC detector, PEEK

The Streamline™ UV1 detector offers an excellent signal-to-noise ratio, making it ideal for demanding applications.

<p>Watrex Streamline™ UV1 programmable UV/VIS detector exhibits excellent <strong>signal-to-noise</strong> ratio for even the most demanding applications. It is equipped with standard analytical cell. It has a <strong>2-lamp source</strong> (deuterium + tungsten), <strong>variable wavelength detector configuration</strong> suitable for operation between <strong>190 and 800 nm</strong>.</p><blockquote><p class="cke-user_style-important"><strong>Price 4 788 € ex VAT</strong></p></blockquote><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Watrex_Watrex_Streamline_UV_1_UV_VIS_detector_686c6c9937.jpg" alt="Watrex: Streamline™ UV1 detector, PEEK" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Watrex_Watrex_Streamline_UV_1_UV_VIS_detector_686c6c9937.jpg 245w," sizes="100vw" width="945" height="436"></p><h3><strong>TECHNICAL SPECIFICATION</strong></h3><ul><li><strong>Light Source:</strong> Deuterium Lamp, Tungsten Lamp</li><li><strong>Wavelength Range:</strong> 190 – 800 nm</li><li><strong>Baseline Noise:</strong> 1 x 10-5 AU (@240 nm, 2 sec. risetime)</li><li><strong>Baseline Drift:</strong> better than 3 x 10-4 AU/h</li><li><strong>Wavelenghth Reproducibility:</strong> ± 0.1 nm</li><li><strong>Linearity:</strong> better than 2.5 AU</li><li><strong>Analog Output:</strong> 1x 1 V</li></ul><p><strong>You can also visit this product on the </strong><a target="_blank" rel="noopener noreferrer" href="https://watrex.com/obchod/chromatography/detectors/uv-vis-detectors/streamline-uv1-detector-2/?utm_source=labrulez&amp;utm_medium=propagace&amp;utm_campaign=labrulez_produkty"><strong>Watrex website</strong></a><strong>.</strong></p>

KNAUER AZURA® Compact Prep HPLC System

The AZURA® Prep Compact system is the perfect start into preparative chromatography. With the complete, semi-preparative HPLC system you master your isocratic purification tasks.

<p class="cke-user_style-important"><strong>AZURA® Preparative HPLC Customized purification</strong></p><p><strong>AZURA® preparative systems are the perfect solution for frequently changing separation tasks – from milligram to kilogram scale. Design your AZURA preparative system to your needs and combine flexibility and reliability.</strong></p><h5><strong>Customaze your system</strong></h5><p data-start="0" data-end="169">AZURA® Prep systems are tailor-made for you. Configure your system from injection to detection and choose between different materials, flow rates, valves, and detectors.</p><h5><strong>Easy scale up from compact to pilot systems</strong></h5><p data-start="171" data-end="344">Due to the flexible design of our devices, you can easily change parts like pump heads or flow cells and integrate all components of the compact into the pilot-scale system.</p><h5><strong>Stacked injections, peak &amp; solvent recycling</strong></h5><p data-start="346" data-end="525" data-is-last-node="" data-is-only-node="">AZURA® Prep systems can be used for special separation modes like peak recycling and stacked injections. We help you to configure your system and choose the best software for you.</p><h3><strong>Flexibility and performance</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg" alt="KNAUER AZURA® Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg 245w," sizes="100vw" width="1920" height="1059"></p><h2><strong>AZURA® Compact Prep HPLC System</strong></h2><p><strong>The AZURA® Prep Compact system is the perfect start into preparative chromatography. With the complete, semi-preparative HPLC system you master your isocratic purification tasks.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Compact_Prep_HPLC_System_3_863a93178e.jpg" alt="KNAUER: AZURA® Compact Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Compact_Prep_HPLC_System_3_863a93178e.jpg 245w," sizes="100vw" width="1590" height="604"></p><h4><strong>Compact Prep System</strong></h4><p>One manual injection can purify several hundred milligrams at up to <strong>50 ml/min</strong>. Detection takes place via a versatile <strong>UV/VIS detector</strong>. The intuitive preparative <strong>software PurityChrom</strong> controls the compact system and regulates the fraction collection via a <strong>12-port fractionating valve</strong>. Thanks to its compact design, the <strong>AZURA Prep Compact system</strong> finds its place in every laboratory.</p><p><img style="aspect-ratio:1024/742;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Compact_Prep_HPLC_System_48291f6f6e.webp" alt="KNAUER: AZURA® Compact Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Compact_Prep_HPLC_System_48291f6f6e.webp 215w," sizes="100vw" width="1024" height="742"></p><ul><li>Up to <strong>150 bar</strong></li><li>Complete <strong>semi-preparative</strong> isocratic HPLC system with low space requirements</li><li>Up to <strong>10 ml</strong> injection volumes &nbsp;</li><li>Injection valve incl. <strong>500 μl sample loop</strong>&nbsp;</li><li>Collect up to <strong>16 large fractions</strong></li><li>Columns from<strong> 4–20 mm ID</strong></li><li><strong>UV/VIS detector</strong> with one variable wavelength</li><li>Intuitive <strong>PurityChrom®, MobileControl® software</strong></li><li><strong>Compact</strong> and <strong>expandable</strong></li></ul><h3><strong>Easy upgrade without big investment</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Compact_Prep_HPLC_System_4_efed81c462.jpg" alt="KNAUER: AZURA® Compact Prep HPLC System: Easy upgrade without big investment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Compact_Prep_HPLC_System_4_efed81c462.jpg 245w," sizes="100vw" width="1600" height="761"></p><h4><strong>Pilot Prep System</strong></h4><div><div data-message-author-role="assistant" data-message-id="efe48b67-eccf-4ebf-8484-ea6d1f96ed57" dir="auto" data-message-model-slug="gpt-4o"><div><div><p data-start="0" data-end="372" data-is-last-node="" data-is-only-node="">After starting preparative chromatography with the space-saving prep system, the requirements for your purification tasks can quickly increase. The existing <strong>Compact System (50 ml/min)</strong> can be expanded to a <strong>Pilot System (220 ml/min)</strong> by investing in a fraction collector and a preparative pump. All components of the<strong> Compact System</strong> are fully integrated into the<strong> Pilot System</strong>.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Compact_Prep_HPLC_System_2_d713ad0086.jpg" alt="KNAUER: AZURA® Compact Prep HPLC System: Easy upgrade without big investment." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Compact_Prep_HPLC_System_2_d713ad0086.jpg 178w," sizes="100vw" width="1606" height="1407"></p><ul><li>Pilot Ternary gradient HPLC system with injection valve incl. <strong>500 μl sample loop</strong>&nbsp;</li><li><strong>Sample pump with automatic sample selection</strong>&nbsp;</li><li><strong>UV/VIS detector&nbsp;</strong>with one variable wavelength</li><li>Intuitive <strong>PurityChrom®</strong> software</li><li><strong>Fraction collector</strong></li></ul></div></div></div></div>

KNAUER AZURA® Lab Prep HPLC System

The Lab Prep LC system is designed for your more demanding semi-preparative separations. You can customize a highly flexible LC system with the freely combinable components.

<p class="cke-user_style-important"><strong>AZURA® Preparative HPLC Customized purification</strong></p><p><strong>AZURA® preparative systems are the perfect solution for frequently changing separation tasks – from milligram to kilogram scale. Design your AZURA preparative system to your needs and combine flexibility and reliability.</strong></p><h5><strong>Customaze your system</strong></h5><p data-start="0" data-end="169">AZURA® Prep systems are tailor-made for you. Configure your system from injection to detection and choose between different materials, flow rates, valves, and detectors.</p><h5><strong>Easy scale up from compact to pilot systems</strong></h5><p data-start="171" data-end="344">Due to the flexible design of our devices, you can easily change parts like pump heads or flow cells and integrate all components of the compact into the pilot-scale system.</p><h5><strong>Stacked injections, peak &amp; solvent recycling</strong></h5><p data-start="346" data-end="525" data-is-last-node="" data-is-only-node="">AZURA® Prep systems can be used for special separation modes like peak recycling and stacked injections. We help you to configure your system and choose the best software for you.</p><h3><strong>Flexibility and performance</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg" alt="KNAUER AZURA® Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg 245w," sizes="100vw" width="1920" height="1059"></p><h2><strong>AZURA® Lab Prep HPLC System</strong></h2><p><strong>The Lab Prep LC system is designed for your more demanding semi-preparative separations. You can customize a highly flexible LC system with the freely combinable components. With a maximum flow rate of 50 ml/min, it is possible to separate up to several hundred milligrams per run.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Lab_Prep_HPLC_System_2_1e66bf8898.jpg" alt="KNAUER: AZURA® Lab Prep HPLC System ." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Lab_Prep_HPLC_System_2_1e66bf8898.jpg 245w," sizes="100vw" width="1588" height="615"></p><ul><li data-start="93" data-end="156"><strong data-start="95" data-end="154">Lab Prep HPLC system with binary high-pressure gradient</strong></li><li data-start="157" data-end="193">Up to 200 bar operating pressure</li><li data-start="194" data-end="263">Injection volumes up to 10 ml via sample pump or manual injection</li><li data-start="264" data-end="329"><strong data-start="266" data-end="286">Injection system</strong>: Injection valve with 500 μl sample loop</li><li data-start="330" data-end="400"><strong data-start="332" data-end="352">Detection system</strong>: UV/VIS detector with one variable wavelength</li><li data-start="401" data-end="453">Supports UVD (VWD, MWD, DAD), RID, FLD, ELSD, MS</li><li data-start="454" data-end="513"><strong data-start="456" data-end="476">Column selection</strong>: Compatible with 4–20 mm column ID</li><li data-start="514" data-end="571"><strong data-start="516" data-end="539">Fraction collection</strong>: Automated fraction collector</li><li data-start="572" data-end="804"><strong data-start="574" data-end="586">Software</strong>:<ul data-start="592" data-end="804"><li data-start="592" data-end="655">Intuitive PurityChrom® for direct control and visualization</li><li data-start="658" data-end="724">Enhanced preparative LC software with functional visualization</li><li data-start="727" data-end="804">GMP (21 CFR part 11) compliant software and certificates for wetted parts</li></ul></li><li data-start="805" data-end="873"><strong data-start="807" data-end="834">User-friendly operation</strong>: Safe and easy-to-use system control</li></ul><h3><strong>Method transfer from analysis of chamazulen to preparative scale</strong></h3><p>Chamomile plants are known for their medical properties, having among others anti-inflammatory, analgesic, and sedative effects. These are due to the various phenolic compounds, one of them being matricine, which is converted during the distillation process to chamazulene. The characteristic blue color of chamomile essential oils, known as “chamomile blue,” is due to chamazulene. It has anti-inflammatory and antioxidant activity. The present application tested preparative HPLC to purify chamazulene from commercially available “chamomile blue” oil.</p><figure class="image"><img style="aspect-ratio:1920/1920;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Lab_Prep_HPLC_System_afb37010e0.webp" alt="KNAUER: AZURA® Lab Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Lab_Prep_HPLC_System_afb37010e0.webp 156w," sizes="100vw" width="1920" height="1920"></figure><h3><strong>Results</strong></h3><h5><strong>Fractionation/Purification</strong></h5><p data-start="0" data-end="318">The separation of chamazulene was optimized on an analytical scale, and the two-step gradient method was transferred to a preparative scale. Chamazulene purification was performed on a <strong>C18 250×20 mm column at 25 ml/min</strong>. Fractionation of chamazulene was conducted using the threshold function of <strong>PurityChrom software</strong>.</p><figure class="image"><img style="aspect-ratio:936/701;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Lab_Prep_HPLC_System_3_4403900c2b.jpg" alt="KNAUER: AZURA® Lab Prep HPLC System: Fig. 1: Chromatogram of preparative separation of chamazulene blue, collected fraction highlighted in red, 1 ml sample injection" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Lab_Prep_HPLC_System_3_4403900c2b.jpg 209w," sizes="100vw" width="936" height="701"></figure><p data-start="320" data-end="552">The collected fraction was analyzed by analytical HPLC and revealed nearly <strong>100% purity</strong>. Chromatogram overlay of the fraction, chamazulene standard, and the sample clearly showed the successful purification of chamazulene (Fig. 2).</p><figure class="image"><img style="aspect-ratio:881/457;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Lab_Prep_HPLC_System_4_4964fc9d9a.jpg" alt="KNAUER: AZURA® Lab Prep HPLC System: Fig. 2: Chromatogram overlay" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Lab_Prep_HPLC_System_4_4964fc9d9a.jpg 245w," sizes="100vw" width="881" height="457"></figure><p data-start="554" data-end="692" data-is-last-node="" data-is-only-node="">Furthermore, the <strong>comparison </strong>of the fraction spectra and chamazulene spectra (Fig. 3) confirmed that the purified fraction was chamazulene.</p><figure class="image"><img style="aspect-ratio:963/484;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Lab_Prep_HPLC_System_5_117b12a2eb.jpg" alt="KNAUER: AZURA® Lab Prep HPLC System: Fig. 3: Spectral view of fraction (-) and of chamazulen standard (-)" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Lab_Prep_HPLC_System_5_117b12a2eb.jpg 245w," sizes="100vw" width="963" height="484"></figure>

KNAUER AZURA® Pilot Prep HPLC System

Choose the Pilot Prep LC system if you want to increase your productivity even more. As for the AZURA Lab Prep LC system you can freely build up your system. Flow rates up to 1 000 ml/min and loads up to several grams are possible.

<p class="cke-user_style-important"><strong>AZURA® Preparative HPLC Customized purification</strong></p><p><strong>AZURA® preparative systems are the perfect solution for frequently changing separation tasks – from milligram to kilogram scale. Design your AZURA preparative system to your needs and combine flexibility and reliability.</strong></p><h5><strong>Customaze your system</strong></h5><p data-start="0" data-end="169">AZURA® Prep systems are tailor-made for you. Configure your system from injection to detection and choose between different materials, flow rates, valves, and detectors.</p><h5><strong>Easy scale up from compact to pilot systems</strong></h5><p data-start="171" data-end="344">Due to the flexible design of our devices, you can easily change parts like pump heads or flow cells and integrate all components of the compact into the pilot-scale system.</p><h5><strong>Stacked injections, peak &amp; solvent recycling</strong></h5><p data-start="346" data-end="525" data-is-last-node="" data-is-only-node="">AZURA® Prep systems can be used for special separation modes like peak recycling and stacked injections. We help you to configure your system and choose the best software for you.</p><h3><strong>Flexibility and performance</strong></h3><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg" alt="KNAUER AZURA® Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Prep_HPLC_System_90fef641c3.jpg 245w," sizes="100vw" width="1920" height="1059"></p><h2><strong>AZURA® Pilot Prep HPLC System</strong></h2><p><strong>Choose the Pilot Prep LC system if you want to increase your productivity even more. As for the AZURA Lab Prep LC system you can freely build up your system. Flow rates up to 1 000 ml/min and loads up to several grams are possible. Optional peak and solvent recycling can be set up to increase separation power and reduce separation costs significantly</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_5_f41d299a7c.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_5_f41d299a7c.jpg 245w," sizes="100vw" width="1492" height="597"></p><ul><li data-start="1517" data-end="1580"><strong data-start="1519" data-end="1580">Pilot Prep HPLC system with binary high-pressure gradient</strong></li><li data-start="1581" data-end="1630">Binary or ternary HPG/LPG, isocratic flow modes</li><li data-start="1631" data-end="1661">Flow rates up to 1000 ml/min</li><li data-start="1662" data-end="1695">20-150 mm recommended column ID</li><li data-start="1696" data-end="1799"><strong data-start="1698" data-end="1718">Injection system</strong>: Sample pump with injection valve for automated handling of large sample volumes</li><li data-start="1800" data-end="1931"><strong data-start="1802" data-end="1822">Detection system</strong>: Supports UVD (VWD, MWD, DAD), RID, FLD, ELSD, MS with split-free UV detection and various flow cell designs</li><li data-start="1932" data-end="2040"><strong data-start="1934" data-end="1957">Fraction collection</strong>: 12-port fractionation valve for automated collection of small and large fractions</li><li data-start="2041" data-end="2080">Stacked injection for high-throughput</li><li data-start="2081" data-end="2156"><strong data-start="2083" data-end="2095">Software</strong>: Intuitive PurityChrom® for direct control and visualization</li><li data-start="2157" data-end="2259"><strong data-start="2159" data-end="2184">Regulatory compliance</strong>: GMP (21 CFR part 11) compliant software and certificates for wetted parts</li><li data-start="2260" data-end="2381"><strong data-start="2262" data-end="2285">Efficiency features</strong>: Solvent recycling to reduce costs and environmental impact, peak-recycling for high resolution</li><li data-start="2382" data-end="2486"><strong data-start="2384" data-end="2416">High-throughput applications</strong>: Liquid handler enables purification of peptides and oligonucleotides</li></ul><h3><strong>Improved purity by combining online SPE with preparative LC</strong></h3><p data-start="0" data-end="365">Steviol glycosides are the main sweetening compounds in <em data-start="56" data-end="75"><i>Stevia rebaudiana</i></em> and are often used as natural sugar substitutes. To enable commercial usage, the plant extracts need to be purified. In this work, preparative online SPE (solid phase extraction) with the AZURA Pilot Prep LC was investigated for improving overall purity by reducing matrix contamination.</p><p data-start="367" data-end="748" data-is-last-node="" data-is-only-node="">The steviol glycoside rebaudioside A is the main compound of interest as it is the sweetest and least bitter compound of the extract. Often, Stevia products contain a mixture of rebaudioside A and stevioside. Developing a purification method with a high yield of rebaudioside A, minimal stevioside impurities, and high throughput increases the economic output of Stevia production.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_87addb89e7.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_87addb89e7.jpg 159w," sizes="100vw" width="1689" height="1656"></p><h3><strong>Results</strong></h3><p>Fig. 1 shows the batch LC without online SPE. The matrix peak (1-5 min) negatively affects the separation abilities.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_2_625c29b0f1.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System : Overload experiments on preparative column, 200 μL (red), 500 μL (blue), 2000μL (green); 1) rebaudioside A, 2) stevioside, blue bars – matrix, 25°C, 22 ml/min." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_2_625c29b0f1.jpg 243w," sizes="100vw" width="893" height="573"></p><p>In comparison, Fig. 2 shows that the automated SPE process significantly decreased the matrix. The fraction analysis revealed that only a small part of the overlapping peak contained nearly pure rebaudioside A; fractions 3-5, approximately 15 mL, had &gt;90% rebaudioside A and &lt;10% stevioside (Fig. 3B).&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_3_3aa3c283b2.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System: Preparative online SPE, 10 mL loading; 1) rebaudioside A, 2) stevioside, blue bars – matrix, 25°C, 22 mL/min." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_3_3aa3c283b2.jpg 243w," sizes="100vw" width="867" height="557"></p><p>The later fractions contained high amounts of stevioside but also still rebaudioside A (Fig. 3C). The results showed that purification of highly pure rebaudioside A is possible with an additional online SPE.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AZURA_Pilot_Prep_HPLC_System_4_e0816cbf59.jpg" alt="KNAUER: AZURA® Pilot Prep HPLC System: Fraction analysis of preparative online-SPE purification (Fig.2) of rebaudioside A (1) and stevioside (2); a) F3 (blue), F4 (red), F5 (green), F6 (light blue); b) F7 (red dashed), F10 (blue dashed), F12 (green sashed), F15 (light blue dashed); c) frac- tionation of target peak, 5 mL fractions." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AZURA_Pilot_Prep_HPLC_System_4_e0816cbf59.jpg 245w," sizes="100vw" width="916" height="550"></p>

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