KNAUER protein purification: The flexible FPLC platform
AZURA® Bio purification systems
complete solutions for FPLC on a minimum footprint: AZURA FPLC systems combine flexibility and reliability. The biocompatible/metal-free FPLC is the perfect choice for your protein purification task.
KNAUER: AZURA® Bio Compact AC FPLC System.
Design your AZURA Bio purification system to fit your needs. Multiple functionalities such as automatic sample injection via autosampler, column switching, buffer and sample selection, and fraction collection enable automation of the purification process.
A wide range of detectors ensures the visibility of target molecules. Various flow rates and compatibility with columns from all vendors provide maximum flexibility. The intuitive PurityChrom® software integrates all the advantages of a versatile purification system.
AZURA® Bio Lab Advanced FPLC System
Gradient-capable FPLC system for a wide range of purifications including automated two-step applications
Protein purification involves most of the times two to three steps:
- capture step
- optional intermediated step
- polishing step
The transition from one to another step generally involves manual interaction and thus is time-consuming. Automation by combining these steps increases the efficiency and optimizes the workflow.
The quick and automated linkage of multiple chromatographic purification steps into one method eliminates manual sample handling and minimizes time spent between steps. This automation strategy can be easily adapted to each purification task.
KNAUER: AZURA® Bio Lab Advanced FPLC System.
Key features
- Flow rate: 0.001–50 ml/min (maximum 50 bar)
- Injection system: Sample injection via loop or sample pump, with automated storage and reinjection of proteins
- Detection system:
- Multichannel UV detector
- Highly sensitive multi-wavelength detector for proteins, DNA/RNA, and peptides
- Conductivity detector
- Fraction collection: XY fraction collector with outlet valve for automated collection of large volumes or numerous fractions
- Column compatibility: Supports up to 7 columns for method development – all FPLC columns supported
- Suitable for Ion-exchange, HILIC, affinity, SEC, and RP
- Automation: Enables challenging protein purification tasks with minimal manual intervention
- Software:
- Intuitive and powerful PurityChrom® software
- 21 CFR Part 11 compliant software
- Additional features:
- Conductivity and pH-monitoring, pressure, and air sensors (optional)
KNAUER: AZURA® Bio Lab Advanced FPLC System.
Two-step purification: Special multi-column chromatography solutions
Designed and optimized for all FPLC applications such as affinity, ion exchange, hydrophobic interaction and size exclusion chromatography. In addition, you can perform dedicated twoSTEP applications.
KNAUER: AZURA® Bio Lab Advanced FPLC System.
Automated two-step purification of mouse IgG antibodies
KNAUER: AZURA® Bio Lab Advanced FPLC System: The affinity chromatography step was automatically combined with a gel filtration step to exchange the buffer of the purified mouse IgG antibodies; Phase 1: Column equilibration, Phase 2: Sample injection and washing, Phase 3: Elution of IgG from protein A column, Phase 4: Desalting of IgG.
