What if you could assess a tumor's metastatic potential — without extensive sample prep — directly from tissue? Rachel Wood is working on exactly that.Rachel Wood is a PhD candidate at Queen's University (Kingston, Ontario),&nbsp;working jointly in the labs of Dr. Richard Oleschuk (Chemistry) and Dr. Chris&nbsp;Nicoll (Pathology &amp; Molecular Medicine). Her research applies Ambient Ionization Mass Spectrometry — specifically the Liquid Microjunction Surface Sampling Probe &nbsp;(LMJ-SSP) and Desorption Electrospray Ionization (DESI) — to profile the metabolomics of non-metastatic and metastatic clear cell Renal Cell Carcinoma (ccRCC) cell lines. The long-term goal: a rapid, minimally invasive MS-based prognostic tool for kidney cancer.This is the first reported use of LMJ-SSP to assess metastatic status in ccRCC —&nbsp;and early PCA results are already showing distinct separation between cell line&nbsp; profiles.<h5>In this episode, David and Rachel cover:</h5><ul><li>Why ccRCC is such a resistant biomarker problem despite active genomic, proteomic, and metabolomic research</li><li>The clinical stakes of "diagnosed by accident" — and what a prognostic biomarker actually needs to do for a clinician</li><li>How AIMS complements (not replaces) LC-MS in a discovery-phase cancer workflow</li><li>LMJ-SSP vs. DESI: what each technique sees that the other misses, including spatial resolution differences (~1mm vs. ~50µm)</li><li>Why Rachel runs LMJ-SSP on both a triple quad and a Q-TOF — and what each instrument contributes to the same research question</li><li>How her lab repurposed a 3D printer as a custom automated XYZ sampling stage with 50µm movement precision</li><li>Managing high-dimensional metabolomics data across two polarities, two cell lines, and two mass analyzers — the pipeline explained</li><li>What unsupervised machine learning (PCA) showed in early results</li><li>The path from cell lines to excised tumor tissue to, ultimately, liquid biopsies</li><li>Advice for analytical chemists considering work at the chemistry–oncology interface</li></ul><h3>Video Transcription</h3>Kidney cancer remains a difficult biomarker problem, particularly when researchers try to distinguish tumors that are likely to behave aggressively from those that are not. In a recent episode of Concentrating on Chromatography, PhD researcher Rachel Wood discussed how she is combining rapid ambient ionization mass spectrometry, LMJ-SSP, DESI, multivariate data analysis, and conventional LC-MS workflows to investigate the molecular differences associated with clear cell renal cell carcinoma and its metastatic potential.<h4>Returning to the research question that started it all</h4>Wood first encountered kidney cancer research during her undergraduate studies. Although she later moved into a more instrumentation-focused master’s project involving breast cancer, the subject of kidney cancer remained unfinished business.When the opportunity arose to choose a PhD project, she returned to the field that had originally sparked her interest in research. She is now conducting her doctoral work at Queen’s University in Kingston, Ontario, in an interdisciplinary environment spanning chemistry, pathology, molecular medicine, computer science, and clinical research.<h4>Why clear cell renal cell carcinoma is such a difficult biomarker problem</h4>Wood’s research focuses on clear cell renal cell carcinoma (ccRCC), which represents the majority of kidney cancer cases discussed in the interview.The difficulty is not a lack of research effort. Genomics, proteomics, and metabolomics studies have all produced promising biomarker candidates. The challenge is that different studies often identify different sets of markers, with relatively little overlap between them.Patient-to-patient biological variability further complicates the picture. A candidate biomarker panel that performs well in one cohort may not reproduce equally well in another. Selecting which features deserve further investigation therefore becomes a major analytical and biological challenge.<h4>From incidental diagnosis to prognostic information</h4>Another difficulty is the way kidney cancer is often discovered.Rather than being detected through a dedicated screening program, tumors may be found incidentally during imaging performed for another reason. According to Wood, the ideal solution would be a diagnostic biomarker capable of detecting kidney cancer early. Such a marker is not currently available within the scope of the work she described.That places additional importance on prognostic biomarkers. Once a tumor has been detected, clinicians need as much information as possible about how aggressive it may be and how likely it is to progress.Wood’s current work is therefore not attempting to declare directly that a particular patient has metastatic disease. Instead, the goal is to understand what changes biologically as a tumor moves from a less aggressive to a more aggressive state.That means asking which molecular pathways change, which mechanisms become altered, and which analytes may eventually provide useful indicators of tumor behavior.<h4>AIMS and LC-MS answer different questions</h4>A central theme of the interview was the relationship between rapid ambient ionization mass spectrometry and conventional LC-MS.Wood is clear that AIMS is not intended to replace LC-MS. Instead, she sees the two approaches as complementary.AIMS provides a rapid way to obtain a broad molecular overview of a sample with minimal preparation. This makes it especially useful during the discovery phase, when the goal is to gather as much molecular information as possible and identify features that distinguish biological groups.LC-MS then becomes valuable when those features need to be separated, characterized more carefully, and quantified with greater confidence.In Wood’s workflow, rapid MS screening can therefore help identify which analytes deserve closer attention before more targeted LC-MS investigations are performed.<h4>What “minimal sample preparation” actually means</h4>The amount of sample preparation required depends strongly on the type of material being analyzed.Wood currently works mainly with cultured human cell lines, which means much of the preparation begins with growing the cells themselves. Depending on the experiment, the cells may then be chemically lysed or subjected to fixation, paraffin embedding, and sectioning before analysis by LMJ-SSP or DESI.She also notes that the possibilities would be different for freshly excised tumor material. In an appropriate perioperative or biosafety laboratory setting, LMJ-SSP could potentially be used to analyze tumor margins more directly.The practical meaning of “minimal preparation” is therefore highly dependent on the sample and the analytical question being asked.<h4>Why combine LMJ-SSP and DESI?</h4>Rather than choosing between LMJ-SSP and DESI, Wood uses the two approaches because they provide different types of information.LMJ-SSP offers rapid, localized analysis and can accommodate samples with uneven surface topography, which is particularly useful for excised tissue.DESI, by contrast, provides substantially higher spatial resolution. In the workflow described in the interview, DESI can achieve spatial resolution on the order of tens of micrometers, whereas LMJ-SSP operates at a much larger sampling scale depending on probe design.That higher spatial resolution is important when moving from simplified cell-line models toward actual tumor tissue, where the molecular organization of different regions may reveal biologically relevant differences.Combining both techniques therefore enables researchers to balance speed, surface compatibility, and spatial information.<h4>Two mass analyzers for two parts of the same problem</h4>Wood also uses two different mass spectrometers in her LMJ-SSP work.Most of the discovery work is carried out using a triple quadrupole mass spectrometer, particularly because her project focuses strongly on lower-mass analytes and small molecules.Once statistically important analytes have been identified, a Q-TOF instrument can be used for MS/MS experiments and tentative structural identification.The two instruments therefore address different stages of the workflow: one supports broad discovery and comparison, while the other provides additional structural information needed to connect selected features with possible metabolic pathways.<h4>Turning a 3D printer into an automated sampling platform</h4>One of the most unusual elements of the project is the automated sampling system used with LMJ-SSP.Historically, LMJ-SSP measurements could be performed manually or with custom-built XYZ positioning stages. Wood’s laboratory instead adapted a 3D printer into an automated positioning system.Custom software controls the printer and moves the sampling probe with precise control over its X, Y, and Z positions. According to Wood, the system enables movements down to approximately 50 µm and also controls parameters such as the contact time between the probe and sample and the delay between consecutive sampling events.This level of automation improves reproducibility because variables that would otherwise depend on manual operation can be controlled systematically.Even the Z-position can be programmed, allowing the probe to operate at constant or varying heights depending on the sample surface.<h4>Managing high-dimensional MS data</h4>The experimental design creates a large data-analysis challenge.Wood is working with multiple cell lines, both ionization polarities, and more than one mass analyzer. To maintain traceability, she analyzes the data in clearly defined subsets.A typical workflow might begin with one mass analyzer and one polarity. Within that condition, the lower-aggressiveness and higher-aggressiveness cell models can then be compared directly.The data processing pipeline includes steps such as:<ul><li>binning,</li><li>normalization,</li><li>transformation,</li><li>dimensionality reduction,</li><li>identification of discriminating molecular features.</li></ul>Once relevant features are identified, MS/MS analysis can be used to investigate them further. The same process is then repeated across other polarities and instruments.<h4>Unsupervised machine learning reveals distinct molecular profiles</h4>Machine learning is particularly valuable because the AIMS datasets are highly dimensional, containing very large numbers of molecular features across multiple samples.Wood’s initial analysis has included principal component analysis (PCA), an unsupervised dimensionality-reduction technique.The early results are encouraging: the non-metastatic and metastatic cell-line models separate clearly in PCA space.That does not itself identify a definitive biomarker panel, but it indicates that the two biological states have distinguishable molecular profiles even though both belong to the same kidney cancer subtype.This provides an important foundation for identifying which specific metabolites or molecular pathways may be responsible for the separation.<h4>Testing LMJ-SSP for metastatic status</h4>A particularly novel aspect of Wood’s project is the use of LMJ-SSP to investigate differences associated with metastatic status.She had previously worked on distinguishing healthy and cancerous tissues, where the biological differences are relatively large. Comparing two states within the same cancer type is more demanding because the molecular changes may be much subtler.The scientific motivation, however, is strong. Tumors discovered at later stages carry greater concern for metastatic progression, making it important to understand the molecular changes associated with increasingly aggressive behavior.For Wood, this made the methodological risk worthwhile: the analytical challenge directly addresses an area where better prognostic information could ultimately have clinical value.<h4>A long-term goal: rapid MS-based prognostic testing</h4>The long-term vision extends beyond cell-line research.Wood is interested in whether rapid, minimally invasive mass spectrometry could eventually contribute to clinical decision-making.One possibility would be identifying a diagnostic biomarker in a liquid biopsy, potentially using blood or urine. A urine-based marker capable of contributing to earlier detection would be particularly attractive for kidney cancer.For prognostic applications, the goal would be slightly different. Once a tumor is discovered, molecular information could help characterize its likely behavior and provide clinicians with additional evidence when deciding between immediate treatment, surgery, or other management strategies.Wood emphasizes that such a tool would not replace standard clinical workflows. Instead, it would provide additional molecular information to complement existing diagnostic and pathological approaches.<h4>Why interdisciplinary work matters</h4>Throughout the interview, Wood repeatedly returns to the importance of collaboration.Her research sits at the intersection of analytical chemistry, pathology, oncology, computation, and clinical medicine. No single researcher can remain equally current in all of these fields.Working closely with pathologists, computer scientists, surgeons, medical students, and other specialists therefore provides both scientific expertise and a way to stay connected with developments outside one’s immediate discipline.For Wood, the biological context has also changed how she thinks about analytical chemistry. Developing a new instrument or method is important, but understanding who may ultimately benefit from that analytical capability makes the research more meaningful.That patient-centered perspective is now an integral part of how she approaches the project.<h4>An intersection full of opportunities</h4>Wood describes the interface between analytical chemistry and oncology as an especially exciting area for young researchers.The work can range from troubleshooting instrumentation to interpreting pathology, developing statistical methods, and collaborating across disciplines. That diversity creates constant opportunities to learn and to apply analytical chemistry to clinically relevant questions.Her project illustrates that modern mass spectrometry research is increasingly about more than generating spectra. It involves connecting instrumentation, spatial sampling, multivariate statistics, machine learning, biological interpretation, and clinical needs into a single analytical strategy.And in kidney cancer, where reliable prognostic biomarkers remain difficult to establish, that integrated approach may be particularly valuable.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

AIMS vs. LC-MS for Cancer Diagnostics: ccRCC Metabolomics with Rachel Wood

In the week of September 21, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.<blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars">👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</a></h4>👉 Search and filter in the largest global database with over 6 000 GC, LC, MS and spectroscopy webinars.</blockquote><h5>1. Waters Corporation: Strengthening SPE Workflows for Today’s Laboratory</h5><ul><li>Mo, 21.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6514">Registration</a></li></ul>Learn how to optimize SPE workflows with best practices for sorbent selection, recovery troubleshooting, and reducing matrix effects in modern analytical laboratories.<h5>2. Agilent Technologies: From Crude to Candidate: Scaling Preparative Chromatography with Confidence</h5><ul><li>Mo, 21.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6498">Registration</a></li></ul>Learn how to scale preparative chromatography from crude sample to purified candidate, with practical strategies for method development, fraction collection, and method transfer.<h5>3. SCIEX: Extending the power of LC MS/MS: advanced workflows with QTRAP®</h5><ul><li>Tu, 22.9.2026 14:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6518">Registration</a></li></ul>Discover how MRM³ and QTRAP workflows provide greater selectivity and lower limits of quantification for LC-MS/MS analysis in complex matrices.<h5>4. Technology Networks: Improving Efficiency in Routine PFAS Analysis Using EPA Methods 533 and 1633A</h5><ul><li>Tu, 22.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6517">Registration</a></li></ul>Explore how automated QC monitoring and the SCIEX novus V55 system support high-throughput PFAS quantitation using EPA Methods 533 and 1633A.<h5>5. Phenomenex: Expanding the Evidence: New Data for More Reliable PFAS Analysis</h5><ul><li>We, 23.9.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6522">Registration</a></li></ul>Discover how new analytical data and contamination control strategies can improve baseline performance, sensitivity, and reliability in PFAS analysis.<h5>6. SCIEX: How is capillary electrophoresis being used to analyze DNA and RNA</h5><ul><li>We, 23.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6521">Registration</a></li></ul>Explore how capillary electrophoresis supports DNA and RNA analysis, from plasmid quality assessment to mRNA impurity profiling, viral vector characterization, and oligonucleotide workflows.<h5>7. Thermo Fisher Scientific: A novel monodisperse supermacroporous reversed-phase platform for comprehensive nucleic acid analysis across a broad size range</h5><ul><li>We, 23.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6476">Registration</a></li></ul>Discover how a novel monodisperse supermacroporous reversed-phase platform enables comprehensive nucleic acid analysis across a broad size range, from short oligonucleotides to mRNA.<h5>8. Waters Corporation: The Next Frontier in Nitrosamines Testing: Detection and Risk Assessment in Packaging and IV Infusion Bags</h5><ul><li>We, 23.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6520">Registration</a></li></ul>Learn how LC-MS/MS supports nitrosamine detection and risk assessment in packaging materials while addressing extractables and leachables challenges in IV infusion bags.<h5>9. MDPI: Membrane Technologies for Water and Food Sustainability</h5><ul><li>We, 23.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6561">Registration</a></li></ul>Explore membrane technologies for water and food sustainability, international research collaboration, and opportunities within the CYTED Program.<h5>10. Agilent Technologies: Unlocking Lab Productivity: Automation Opportunities for LC/MS Laboratories</h5><ul><li>We, 23.9.2026 18:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6495">Registration</a></li></ul>Discover how automation can increase productivity in LC/MS laboratories by reducing manual tasks, improving workflow efficiency, and supporting scalable analytical operations.<h5>11. Thermo Fisher Scientific: Comparison of biocompatible and inert UHPLC systems for LC-UV quantitation of large RNA</h5><ul><li>Th, 24.9.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6477">Registration</a></li></ul>Learn how the Vanquish Amplify system minimizes metal interactions during LC-UV analysis of large RNA and explore the performance of biocompatible and inert UHPLC systems.<h5>12. Agilent Technologies: Next-Generation Pathogen Genomics: The Long-Read Advantage</h5><ul><li>Th, 24.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6526">Registration</a></li></ul>Explore long-read sequencing and automated target enrichment in pathogen genomics and learn how Agilent SureSelect and Magnis NGS support improved pathogen detection.<h5>13. Evosep Biosystems: Advancing Plasma Proteomics</h5><ul><li>Th, 24.9.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6523">Registration</a></li></ul>Discover advances in plasma proteomics and learn how high-throughput analytical workflows can accelerate biomarker discovery and cancer research.<h5>14. Waters Corporation: Applications of Multi-Angle Light Scattering for Biotherapeutic Characterization</h5><ul><li>Th, 24.9.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/webinar/6430">Registration</a></li></ul>Learn how multi-angle light scattering (MALS) supports confident characterization of monoclonal antibodies, antibody-drug conjugates, and AAV viral vectors.

Webinars LabRulezLCMS Week 39/2026

Forced degradation analysis of recombinant adeno-associated virus serotype 8 based on analytical anion exchange chromatography coupled to orthogonal characterization

Article

Webinars

Scientists

Scientific article

Interview

Science and research

Video

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Phenomenex Strata C18-E Solid Phase Extraction (SPE)

Strata C18-E is a silica-based end-capped C18 solid phase extraction (SPE) sorbent.

Strong Hydrophobic Retention Solid Phase Extraction (SPE)Strata C18-E is a silica-based end-capped C18 solid phase extraction (SPE) sorbent that offers strong hydrophobic retention with negligible secondary polar interactions from active silanol groups. Strata C18-E can be best used for the extraction of hydrophobic or polar organic analytes from aqueous matrices. In addition, Strata SPE sorbents are manufactured with the highest purity and extensively tested to give clean extracts.Strata C18-E is compatible with various formats, including C18 SPE cartridges. Our C18 SPE products are synonymous with quality, offering the highest purity and undergo rigorous testing to deliver consistently clean extracts for your analytical needs.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_C18_E_748c192b52.webp" alt="Phenomenex: Strata-C18-E" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_C18_E_748c192b52.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Surface Area (m²/g): 500, m²/g</li><li>Separation Mode: Reversed Phase</li><li>Pore Size: 70, 100</li><li>Particle Size: 20, 55</li><li>Recommended Use: Extraction of hydrophobic molecules</li></ul><h4>Starting Method</h4><h5>Strata Reversed Phase</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL DI Water</li><li>Load: Pretreated sample</li><li>Wash: 1 mL 5% Methanol in DI Water, dry under vacuum for 2-5 min</li><li>Elute: 1 mL Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex β-Gone β-Glucuronidase Removal 96-well plates and tubes

β-Gone β-Glucuronidase Removal 96-well plates and tubes are designed to target and remove β-glucuronidase from hydrolyzed urine samples without requiring additional time or method development.

β-Gone™ offers a simple, fast, and highly targeted method to remove β-Glucuronidase from urine samples prior to analysis. Within 1 minute, with no necessary method development, your samples will be ready for analysis.<h4>Rapid Cleanup of Hydrolyzed Urine</h4><ul><li>Increase HPLC/UHPLC Column Lifetime</li><li>Reduce Mass Spec Maintenance</li><li>Maintain the Selectivity of Your HPLC/UHPLC Columm</li></ul><h3>Available Formats &amp; Protocols:</h3><h5>β-Gone Plus 96-Well Plates Protocol:</h5><ul><li>Follow β-glucuronidase vendor specifications and add appropriate amount of enzyme and urine to the well*&nbsp;<ul><li>Please note: Do not use more than 10% organic solution for the incubation and ensure that &nbsp;organic solution is added last.</li></ul></li><li>Add 133 µL of 0.1% Formic acid in Methanol</li><li>Initiate vacuum or positive pressure (2-5 psi) and collect eluent to inject**</li></ul><h5>β-Gone 96-Well Plates and 1 mL Tubes Protocol:</h5><ul><li>Dilute 200 µL of urine hydrolysate with 133 µL of 0.1% Formic acid in Methanol</li><li>Load diluted sample onto plate/tube and apply vacuum &nbsp;or positive pressure (2-5 psi) and collect eluent</li><li>Inject eluent**</li></ul><h5>2 mL Centrifuge Tubes Protocol:</h5><ul><li>Tap tube prior to use to consolidate sorbent&nbsp;</li><li>Combine 200 µL of Urine Hydrolysate with 133 µL of 0.1 % Formic acid in Methanol&nbsp;</li><li>Load into the centrifuge tube, cap, and invert 10x. Vortex for 30 seconds.</li><li>Centrifuge tube at 14000 rpm for 10 min (16000 xg RCF)&nbsp;</li><li>Collect supernatant to inject for analysis** &nbsp;</li></ul>*For urine samples &lt; 200 µL, use 30 mg β-Gone Plus Plate&nbsp; For urine samples &nbsp;≥ 200 µL, use 60 mg β-Gone Plus Plate&nbsp; **Depending on injection volume, starting %B (organic) in mobile phase, and column dimensions, a dilution of 1:1 with aqueous mobile &nbsp;phase may be required to prevent peak distortion of early eluting compounds.&nbsp;<h4>Ordering Information</h4>β-Gone β-Glucuronidase Removal Products<ul><li>8B-S139-TAK: 1 mL Tubes, Recombinant Enzyme, 100/Box</li><li>8B-S322-DAK: 1 mL Tubes, Non-Recombinant Enzyme, 100/Box</li><li>8E-S139-TGA: 96-Well Plate, Recombinant Enzyme, 1/Box&nbsp;</li><li>8E-S322-DGA: 96-Well Plate, Non-Recombinant Enzyme, 1/Box&nbsp;</li><li>8N-S323-TUK: 2 mL Centrifuge Tubes, Recombinant and Non-Recombinant Enzyme, 100/Box&nbsp;</li><li>8E-S323-TGA: 96-Well Plate Plus 30 mg/well, Recombinant/Non-Recombinant Enzyme, 1/Box</li><li>8B-S323-UGA: 96-Well Plate Plus 60 mg/well, Recombinant/Non-Recombinant Enzyme, 1/Box &nbsp;</li></ul>

Phenomenex Kinetex Core-Shell Polar C18 LC Columns

The Kinetex® 2.6 µm Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase.

The Kinetex® 2.6 µm Polar C18 is a core-shell based particle with a polar modified surface and a C18 alkyl phase. This versatile column can easily upgrade existing fully porous 5 µm and 3 µm methods to shorten run time, increase sensitivity, and even provide greater resolution with much higher efficiency levels. Combined with being 100 % aqueous stable, the Kinetex Polar C18 is a unique column that combines performance and selectivity.<ul><li>Compatible with All Analytical HPLC/UHPLC Systems</li><li>L:1 C18 with Unique Polar Retention</li><li>Compatible with 100 % Aqueous Mobile Phases</li><li>USP: L1</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_polar_C18_60ab4fb700.webp" alt="Phenomenex: Kinetex-polar-C18" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_polar_C18_60ab4fb700.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 9 (effective), 9 (effective) %</li><li>Particle Size (μm): 2.6, 3</li><li>Recommended Use: Combined C18 and polar modified surface that provides polar and non-polar retention alongside 100% aqueous stability</li></ul><h4>C18 and Polar Selectivity</h4>Combined C18 and polar modified surface that provides polar and non-polar retention alongside 100 % aqueous stability.<h4>Increase Polar Retention and Hydrophobic Selectivity</h4>Increase polar selectivity without sacrificing hydrophobic selectivity with the Kinetex Polar C18. The Kinetex Polar C18 is an excellent all-purpose, 100% aqueous stable phase for use with multi-compound mixes that contain polar and non-polar compounds, or even single class methods that have closely related compounds, impurities, or metabolites.<h4>Kinetex Where Performance Meets the Right Selectivity</h4>Quickly improve the productivity and performance of your existing methods and utilize the core-shell advantage to deliver performance you need at backpressures the system can handle. Select from a variety of column selectivity to find the right phase for the application.<h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Kinetex XB-C18 Core Shell Columns

Di-isobutyl side chains differentiate this C18 column. Low ligand density and an inactive surface make this column a great hydrogen bond acceptor. 

<h4>A Unique Core-Shell Selectivity</h4>Di-isobutyl side chains differentiate this C18 column. Low ligand density and an inactive surface make this column a great hydrogen bond acceptor. This phase will demonstrate improved peak shape and increased retention of acidic compounds.<ul><li>Compatible with all Analytical HPLC/UHPLC Systems</li><li>Unique C18 Retention (USP: L1)</li><li>Improved Selectivity of Acidic compounds</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_XB_C18_Core_Shell_HPLC_Column_411f3ff1a8.webp" alt="Phenomenex: Kinetex XB-C18 Core Shell HPLC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_XB_C18_Core_Shell_HPLC_Column_411f3ff1a8.webp 245w," sizes="100vw" width="500" height="150"><ul><li>Carbon Load: 10 (effective), 10.0, null %, 10 (effective) %, 10.0 %</li><li>Surface Area (m²/g): 200</li><li>Particle Size (μm): 1.7, 2.6, 3.5, 5</li><li>Recommended Use: Superior peak shape and enhanced separation of basic compounds under neutral and acidic conditions</li></ul><h4>Selectivity for Acidic Compounds</h4>This unique C18 phase yields increased hydrogen bonding with hydrophobic selectivity‚ resulting in improved peak shape for basic compounds and increased retention of acidic compounds.<h4>Complementary Core-Shell C18 Phases</h4>Ultra-high performance columns like Kinetex will give you very narrow peaks, but without the right selectivities you are left with very narrow, overlapping peaks. The phases offered in the Kinetex column line are complementary to one another, so the spectrum of selectivity your separations require is covered.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Kinetex_C18_vs_XB_C18_fe7c0252f4.jpg" alt="Phenomenex: Kinetex C18 vs. XB-C18" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Kinetex_C18_vs_XB_C18_fe7c0252f4.jpg 165w," sizes="100vw" width="902" height="855"><h4>Manipulate Acidic and Basic Compound Retention</h4>Find the right phase selectivity for your application by investigating the diverse selectivity availability of the Kinetex Core Shell HPLC and UHPLC columns. Where reliable performance meets unique selectivity.<blockquote>Stationary phase Kinetex XB-C18 is provided in these column types: analytical and preparative</blockquote><h3>Kinetex Core-Shell Technology Columns</h3>Kinetex Core-Shell Technology delivers dramatic improvements in efficiency over conventional fully porous media, which can be leveraged to increase resolution, significantly improve productivity, reduce solvent consumption, and decrease costs. Whether you are running high-performance liquid chromatography (HPLC) or ultra-high performance liquid chromatography (UHPLC) methods, the Kinetex core-shell family can deliver shockingly improved performance over the current column you are using. Phenomenex designs, manufactures, and sells its very own silica and organo-silica core-shell particles.Advantages of Kinetex HPLC Columns<ul><li>Performance gains on ANY liquid chromatography system</li><li>System-to-system and lab-to-lab method portability</li><li>Improve the productivity of older, established methods.</li></ul><h4>How Does Core-Shell Technology Work?</h4>Core-shell technology in chromatography is a revolutionary approach that enhances the efficiency of HPLC systems while minimizing costs. Core-shell particles are designed with a solid, non-porous silica core surrounded by a porous outer layer. This unique structure allows for faster analyte mass transfer between the mobile and stationary phases, as diffusion occurs only through the thin, porous shell rather than the entire particle. This minimizes band broadening, leading to higher separation efficiency and sharper peaks.The consistency in size and shape of core-shell particles further enhances separation efficiency by reducing variability in analyte movement. As a result, core-shell HPLC columns provide high efficiency and resolution at lower backpressures, making them an ideal choice for labs looking to improve their existing HPLC systems without the need for costly UHPLC equipment.Core-shell technology thus offers a cost-effective alternative to upgrading to UHPLC systems, enabling labs to maximize the efficiency of their current HPLC setups. The formation of these particles through advanced sol-gel processing techniques ensures uniformity and reliability, making them a valuable tool in modern chromatography. By using the Kinetex HPLC column, laboratories can achieve faster analysis times and higher productivity while maintaining excellent separation performance.<h3>Kinetex Core-Shell Performance on Any LC System</h3>The 5 versatile particle sizes allow you to select the best fit for your instrument.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg" alt="Phenomenex: Five versatile particle sizes of Kinetex Core-Shell Technology Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_5_particle_sizes_bbed75c36f.jpg 245w," sizes="100vw" width="1341" height="333"><h3>Particle Chemistry</h3>Using sol-gel processing techniques that incorporate nano-structuring technology, a durable, homogeneous porous shell is grown on a solid silica core to create a Kinetex Core-Shell particle. This particle morphology results in less band broadening for all four sources of UHPLC band broadening compared to fully porous particles and thus delivers extremely high efficiencies.<ul><li>Optimized for Ultra-High Performance</li><li>Superior Core-Shell Quality</li><li>Core-Shell Scalability and Portability</li></ul>Kinetex particles are characterized by an exceptionally consistent size distribution, with particles being nearly monodispersed, meaning they are uniform in size. This precise uniformity plays a crucial role in minimizing variability within the column, leading to enhanced separation efficiency and sharper peaks.The nearly monodispersed nature of these particles ensures that the flow of the mobile phase remains consistent, reducing the likelihood of irregularities that can impact results. As a result, Kinetex columns deliver highly reliable and reproducible performance, making them ideal for achieving consistent analytical outcomes acrLC Kinetex Core-Shell Technolooss various applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg" alt="Phenomenex: Kinetex Polar C18 vs Fully Porous phase" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_HPLC_and_UHPLC_Kinetex_Core_Shell_Technology_Columns_Kinetex_Polar_C18_vs_Fully_Porous_phase_a2729b64e5.jpg 245w," sizes="100vw" width="1376" height="581"><h3>Core-shell vs Fully Porous</h3><h4>High-Efficiency‚ High-Density Particle</h4>The combination of a consistent, solid, high-density core along with proprietary column packing technologies in the Kinetex HPLC column ensures optimum bed structure and high column performance. This carefully designed core structure promotes uniform packing of the particles, which is essential for minimizing band-broadening effects that can arise from Eddy Diffusion.The manufacturing process of Kinetex columns is tightly controlled, focusing on factors such as particle size distribution, surface and bonding homogeneity, and packing quality. This meticulous engineering leads to uniform packing and minimized band-broadening, resulting in superior separation efficiency.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg" alt="Phenomenex: Core-shell vs Fully Porous.jpg" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Core_shell_vs_Fully_Porous_60601b6fa3.jpg 245w," sizes="100vw" width="904" height="506"><h3>Expand Your Core-Shell Selectivity Toolbox</h3>Kinetex core-shell particle LC columns deliver exceptional performance enhancements across any LC system, offering unmatched efficiency and resolution. With a choice of 11 diverse stationary phases, these columns cater to a wide range of chromatographic applications, ensuring optimal separation for various analytes.Additionally, the availability of five versatile particle sizes allows for precise method tailoring, enabling both high-speed and high-resolution separations. Whether you're using HPLC or UHPLC systems, Kinetex columns provide the flexibility and performance needed to achieve superior analytical results.<h3>Top 5 Pros of Kinetex Core-Shell Technology</h3><ul><li>Productivity: Kinetex Core-Shell Technology significantly enhances method productivity with minimal effort. By simply replacing traditional, fully porous columns with Kinetex HPLC columns, users can achieve better resolution, sensitivity, and overall productivity without extensive method redevelopment. This easy switch allows for immediate improvements in chromatographic performance, making it a cost-effective upgrade to existing methods.</li><li>Portability The Kinetex Core-Shell columns are available in a range of particle sizes (1.3 µm to 5 µm), which ensures method portability across different HPLC and UHPLC systems. This scalability allows for consistent retention and selectivity across various particle sizes while accommodating different backpressure levels, making it easier to transfer methods between instruments.</li><li>Performance Kinetex Core-Shell Technology delivers superior chromatographic efficiency compared to fully porous particles. This efficiency boost enables chromatographers to maximize the performance of their HPLC/UHPLC systems, ensuring optimal results with higher resolution and sharper peaks, even under similar method conditions.</li><li>Phases With 11 unique phase selectivities available, Kinetex Core-Shell Technology offers unmatched versatility for method development. This wide selection of phase chemistries allows for tailored separations and simplified optimization, ensuring the best possible results for a wide range of applications.</li><li>Pressure Kinetex Core-Shell columns provide higher efficiency at lower system backpressures compared to fully porous columns. This allows users to achieve superior chromatographic performance on conventional LC instruments without the need for ultra-high-pressure systems, making it a practical choice for routine analyses.</li></ul>

Phenomenex Strata SCX Solid Phase Extraction (SPE)

A benzene sulfonic acid group is bonded to the surface of the silica particle, giving strong cation-exchange selectivity.

Ion-Exchange Solid Phase Extraction (SPE) SorbentA benzene sulfonic acid group is bonded to the surface of the silica particle, giving strong cation-exchange selectivity.<ul><li>Extraction of 1 °, 2 °, and 3 amines</li></ul><figure class="image"><img style="aspect-ratio:791/384;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_SCX_SPE_f35df1035c.jpg" alt="Phenomenex: Strata SCX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_SCX_SPE_f35df1035c.jpg 245w," sizes="100vw" width="791" height="384"></figure><ul><li>Surface Area (m²/g): -</li><li>Separation Mode: Ion Exchange</li><li>Particle Size: 55</li><li>Recommended Use: Extraction of weakly basic compounds</li></ul><h4>Starting Method</h4><h5>Strata SCX – Strong Cation-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Pretreated sample (acidified)</li><li>Wash 1: 1 mL 0.1N HCl in Water</li><li>Wash 2: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5 % NH4OH in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata NH2 Solid Phase Extraction (SPE)

This amino phase offers strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent.

Normal Phase Solid Phase Extraction (SPE)This amino phase offers strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent.<figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_nh2_a7c3599f55.webp" alt="Phenomenex: Strata-NH2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_nh2_a7c3599f55.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li>Surface Area (m²/g): -</li><li>Separation Mode: Normal Phase</li><li>Pore Size: 100</li><li>Particle Size: 25, 55</li><li>Recommended Use: Strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent</li></ul><h4>Starting Method</h4><h5>Strata NH2 – Weak Anion-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Water, pH 6-7</li><li>Load: Pretreated sample, pH 6-7</li><li>Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li>Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min</li><li>Elute Any Acid: 1 mL 5% NH4OH in Methanol</li><li>Elute Weak Acids: 1 mL 5% Formic Acid in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata SAX Solid Phase Extraction (SPE)

The quaternary amine group on this sorbent remains positively charged under all conditions, giving a strong anion-exchange mechanism of retention.

Ion-Exchange Solid Phase Extraction Sorbent (SPE)The quaternary amine group on this sorbent remains positively charged under all conditions, giving a strong anion-exchange mechanism of retention.<figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_sax_470d923b27.webp" alt="Phenomenex: Strata-SAX" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_sax_470d923b27.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li>Surface Area (m²/g): -</li><li>Separation Mode: Ion Exchange</li><li>Particle Size: 55</li><li>Recommended Use: Extraction of weakly acidic compounds</li></ul><h4>Starting Method</h4><h5>Strata SAX – Strong Anion-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL DI Water</li><li>Load: Pretreated sample, pH 6-7</li><li>Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li>Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5% Formic Acid in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata Screen-A Solid Phase Extraction (SPE)

Incorporates the hydrophobic selectivity of a C8 phase and strong anion-exchange for the extraction of acidic drugs from biological matrices.

Mixed-Mode Solid Phase Extraction (SPE) SorbentIncorporates the hydrophobic selectivity of a C8 phase and strong anion-exchange for the extraction of acidic drugs from biological matrices.<figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_Screen_A_f370bdfd48.webp" alt="Phenomenex: Strata Screen-A" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_Screen_A_f370bdfd48.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li>Surface Area (m²/g): -</li><li>Separation Mode: Multi-Mode</li><li>Particle Size: 55</li><li>Recommended Use: Extracts acidic drugs from biological matrices</li></ul><h4>Starting Method</h4><h5>Strata Screen-A Strong Anion-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Water</li><li>Load: Pretreated sample</li><li>Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7</li><li>Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5% Formic Acid in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata Screen-C Solid Phase Extraction (SPE)

Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.

Strong Cation-Exchange Solid Phase Extraction (SPE)Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.<figure class="image"><img style="aspect-ratio:500/150;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Strata_Screen_C_b60bb2b7e4.webp" alt="Phenomenex: Strata Screen-C" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Strata_Screen_C_b60bb2b7e4.webp 245w," sizes="100vw" width="500" height="150"></figure><ul><li>Surface Area (m²/g): 500, m²/g, 500 m²/g</li><li>Separation Mode: Multi-Mode</li><li>Pore Size: 70</li><li>Particle Size: 55</li><li>Recommended Use: Extraction of basic drugs from biological matrixes</li></ul><h4>Starting Method</h4><h5>Strata Screen-C Strong Cation-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Pretreated sample (acidified)</li><li>Wash 1: 1 mL 0.1N HCl in Water</li><li>Wash 2: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5 % NH4OH in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Phenomenex Strata Screen-C GF Solid Phase Extraction (SPE)

Optimized for gravity flow, this sorbent offers hydrophobic selectivity from a C8 phase and strong cation-exchange properties for the extraction of basic drugs.

Strong Cation-Exchange Solid Phase Extraction (SPE)Optimized for gravity flow, this sorbent offers hydrophobic selectivity from a C8 phase and strong cation-exchange properties for the extraction of basic drugs from biological matrixes. Offers the selectivity of Screen-C in a gravity flow particle size for viscous samples.<h4>Starting Method</h4><h5>Strata Screen-C GF Strong Cation-Exchange</h5><ul><li>Condition: 1 mL Methanol</li><li>Equilibrate: 1 mL Acidified Water</li><li>Load: Pretreated sample (acidified)</li><li>Wash 1: 1 mL 0.1N HCl in Water</li><li>Wash 2: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min</li><li>Elute: 1 mL 5 % NH4OH in Methanol</li></ul>Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.

Products from Phenomenex distributed by Phenomenex - page 7

Related content

Forced degradation analysis of recombinant adeno-associated virus serotype 8 based on analytical anion exchange chromatography coupled to orthogonal characterization

Webinars LabRulezLCMS Week 39/2026

AIMS vs. LC-MS for Cancer Diagnostics: ccRCC Metabolomics with Rachel Wood