Normal Phase Solid Phase Extraction (SPE)
This amino phase offers strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent.
Phenomenex: Strata-NH2- Surface Area (m²/g): -
- Separation Mode: Normal Phase
- Pore Size: 100
- Particle Size: 25, 55
- Recommended Use: Strong polar selectivity and hydrogen bonding under normal phase conditions or can be used as a weak anion-exchange sorbent
Starting Method
Strata NH2 – Weak Anion-Exchange
- Condition: 1 mL Methanol
- Equilibrate: 1 mL Water, pH 6-7
- Load: Pretreated sample, pH 6-7
- Wash 1: 1 mL 25mM Ammonium Acetate Buffer, pH 6-7
- Wash 2: 1 mL Methanol, dry under vacuum for 2-5 min
- Elute Any Acid: 1 mL 5% NH4OH in Methanol
- Elute Weak Acids: 1 mL 5% Formic Acid in Methanol
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.
