Strong Cation-Exchange Solid Phase Extraction (SPE)
Incorporates the hydrophobic selectivity of a C8 phase and strong cation exchange for the extraction of basic drugs from biological matrices.
Phenomenex: Strata Screen-C- Surface Area (m²/g): 500, m²/g, 500 m²/g
- Separation Mode: Multi-Mode
- Pore Size: 70
- Particle Size: 55
- Recommended Use: Extraction of basic drugs from biological matrixes
Starting Method
Strata Screen-C Strong Cation-Exchange
- Condition: 1 mL Methanol
- Equilibrate: 1 mL Acidified Water
- Load: Pretreated sample (acidified)
- Wash 1: 1 mL 0.1N HCl in Water
- Wash 2: 1 mL 0.1N HCl in Methanol, dry under vacuum for 2-5 min
- Elute: 1 mL 5 % NH4OH in Methanol
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.
