Learn how free MoNA and MS-DIAL MS/MS libraries can complement NIST26, expand spectral search coverage, and support compound identification in LC-MS/MS workflows.

Explore the free NIST26 demo for integrated LC-MS/MS processing, including MS/MS deconvolution, library searching, test files, and support for NIST, MoNA, and MS-DIAL libraries.

Explore pressurized liquid extraction (PLE), including its principle, instrumentation, step-by-step workflow, and key parameters affecting extraction efficiency.

Explore the NIST26 Identifications folder and learn how XIC traces, MS/MS searches, isotope information and intermediate “check” files support more confident LC-MS/MS identifications.

How can you extend the life of an HPLC column? Proper handling, suitable operating conditions, regular flushing, and correct storage all play an important role.

Learn how to convert LC-MS and GC-MS vendor data to mzML for NIST26 chromatogram processing using ProteoWizard MSConvert, including correct centroiding and Peak Picking settings.

Ion exchange chromatography (IEX) uses differences in charge to separate ionizable compounds effectively. Learn how it works, where it is used, and what its key benefits and limitations are.

Learn how manual and automated Hybrid MS/MS Searches in NIST26 use spectral similarity, mass differences, dForm, and fragmentation to investigate unknown compounds beyond exact library matches.

Discover how fluorescence detection works in HPLC, why it offers exceptional sensitivity and selectivity, and how to optimize FLD for trace analysis in complex samples.

Learn how moving chromatogram results into the NIST Library Search window enables deeper candidate review, flexible precursor settings, and alternative spectral matching strategies.