<h3><strong>Program CEEPC/IPM/CMSC 2022</strong></h3>
<blockquote>
<p><a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/abstractbook_2022_3bec805e4e/abstractbook_2022.pdf"><strong>Complete program including abstracts for download</strong></a></p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Suresh_Jivan_Gadher_cb291006b9/CSHS-Konference-CEEPC-IPM-CMSC-2022-(Suresh-Jivan-Gadher).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 (Suresh Jivan Gadher)"/></p>
<h3><strong>Thursday September 29, 2022</strong></h3>
<h5><strong>8:30 - 10:00 Registration</strong></h5>
<h5><strong>10:00 - 10:10 Opening of the 16th CEEPC, 8th IPM and 10th CMSC</strong></h5>
<h4><strong>10:10 - 11:00 Plenary lecture I: New insights through Single-Cell Proteomics</strong> (Chairperson: Petr Novák)</h4>
<ul>
<li>Karl Mechtler</li>
</ul>
<p>The analysis of single cell proteomes has recently become a viable complement to transcriptomics and genomics studies. Proteins are the main driver of cellular functionality and mRNA levels are often an unreliable proxy of such. Therefore, the global analysis of the proteome is essential to study cellular identities. Both multiplexed and label-free mass spectrometry-based approaches with single cell resolution have lately attributed surprising heterogeneity to believed homogenous cell populations. Even though specialized experimental designs and instrumentation have demonstrated remarkable advances, the efficient sample preparation of single cells still lacks behind.</p>
<p>Here, we introduce the proteoCHIP, optimized for multiplexed single cell proteomics sample preparation at surprising sensitivity and throughput. Sample processing using the cellenONE® robot, allows to reduce final sample volumes to low nanoliters submerged in a hexadecane layer simultaneously eliminating error prone manual sample handling and overcoming evaporation. This results in around 1,500 protein groups per analytical run at remarkable reporter ion signal to noise while reducing or eliminating the carrier proteome. We identified close to 2,600 proteins across 170 multiplexed single cells from two highly similar human cell types. This dedicated loss-less workflow allows to distinguish in vitro co-differentiated cell types of self-organizing cardiac organoids based on indicative markers across 150 single cells. In-depth characterization revealed enhanced cellular motility of endothelial cells and acute myocardium sarcomere organization in cardiomyocytes.</p>
<p>In addition, we evolved a robust and sensitive one-pot label free single cell workflow. By working in standard 384 well plates and compatibility with both, cell sorting in the cellenONE® or using alternatives like a FACS device the need for specialized equipment is obsolete making this workflow easy to use, cheap and accessible to a broader community. By keeping the sample in a hydrated state during proteolytic digestion and addition of DMSO for storage we improved recovery of hydrophobic peptides and boosted ID numbers to more than 1000 proteins from a single cell without match between runs.<br/>In conclusion, our versatile, and semi-automated sample preparation workflows have not only proven to be easily adoptable but are also sufficiently sensitive to drive biological applications of single cell proteomics.</p>
<h5><strong>11:00 - 11:20 Coffee break</strong></h5>
<blockquote>
<h3><strong>Sponsors</strong></h3>
</blockquote>
<blockquote>
<ul>
<li>Bruker Daltonics</li>
<li>Amedis</li>
<li>Merck</li>
<li>Beckman coulter</li>
<li><a href="https://lcms.cz/companies/42"><strong>Pragolab</strong></a></li>
<li><a href="https://lcms.cz/companies/50"><strong>Waters Corporation</strong></a></li>
</ul>
</blockquote>
<blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Sponsors_CEEPC_IPM_CMSC_2022_6f46a9b213/CSHS-Sponsors-CEEPC-IPM-CMSC-2022.webp" alt="CMSC: Sponsors: CEEPC/IPM/CMSC 2022"/></p>
</blockquote>
<h4><strong>11:20 - 13:00 Session I - Method Development</strong></h4>
<ul>
<li>Chairperson: Katarina Davalieva</li>
</ul>
<h5><strong>11:20 - 11:40 Identification of potential cell-surface targets and druggable enzymes in human pheochromocytoma and paraganglioma using classical and membrane-targeting proteomic approaches</strong></h5>
<ul>
<li>Ondřej Vít</li>
</ul>
<p>Integral membrane proteins (IMPs) represent optimal drug targets but are under-represented in standard proteomic analyses due to their amphipathy, lack of trypsin cleavage sites, and low expression levels. To identify tumor-upregualted IMPs and druggable enzymes in rare neuroendocrine tumors – pheochromocytoma and paraganglioma (PPGL), we combined three approaches: 1) hpTC method, where the identification of IMPs is based on their hydrophobic alpha-helices, isolated by proteolytic shaving and re-cleaved with CNBr; 2) two glycocapture methods - lectin entrapment on ultrafilters (N-glyco-FASP), and solid-phase enrichment with hydrazide chemistry (SPEG); and 3) the classical detergent-trypsin approach.<br/>We focused mainly on the high-risk tumors belonging to the so-called cluster 1, characterized by mutations in genes related to citric acid cycle and hypoxia, such as SDHB, VHL, and EPAS1). The classical trypsin-based proteomic approach pointed us toward upregulated soluble druggable enzymes (autotaxin, SHMT2, and Arginase 2) and upregulated cell surface IMPs (including CD146 and CD171). The glycopeptide enrichment approach provided additional potential cell surface targets (CD39), and the hpTC method provided additional IMP targets (e.g., anoctamin-1).<br/>The above-mentioned IMPs and soluble enzymes have all been previously shown to be upregulated in several human cancers and to affect tumor progression directly. Their marked upregulation was confirmed by specific antibodies. Together, this makes these molecules promising candidates for drug targets and/or proteins enabling sensitive tumor imaging.</p>
<h5><strong>11:40 - 12:00 Global SEC-PCP-SILAC mapping reveals protein complexes mediating NF-κB activation in breast cancer</strong></h5>
<ul>
<li>Petr Lapčík</li>
</ul>
<p>NF-κB has essential role in immune response and is associated with lymph node metastasis of luminal A breast tumors (1). Analysis of protein interactome and its changes in response to NF-κB modulation could uncover pro-metastatic mechanisms related to NF-κB. We apply metabolic isotope labeling SILAC, size exclusion chromatography (SEC) and protein correlation profiling (PCP) (2) to construct a network of interactome rearrangement in response to NF-κB modulation in MCF-7 breast cancer cells.</p>
<p>We generated two co-fractionation datasets consisting of 80 fractions from SILAC-labeled and 80 fractions from label-free native MCF-7 lysates with inhibited or native NF-κB activity. LC-MS/MS analysis of SEC fractions using Orbitrap Lumos and Bruker Impact II mass spectrometers quantified 3308 and 5460 protein groups in SILAC and label-free datasets, respectively (FDR = 0.01). Interactome reconstruction using PrinCE (3) detected 7568 interactions among 1520 proteins. Co-elution of subunits of known complexes, such as ribosome, proteasome and MCM, was observed. Modulation of NF-κB was linked to interactome changes of proteins involved in immune response, cell cycle and DNA replication. NF-κB factor RELA interacted with proteins co-eluting with activators of NF-κB and these interactions were modulated by NF-κB inhibition.</p>
<p>Our interaction network represents a complex insight into dynamics of MCF-7 protein interactome associated with NF-κB pathway and could serve as a basis for future studies characterizing NF-κB in breast cancer.</p>
<h5><strong>12:00 - 12:20 Determination of changes in the milk proteome in production and storage of kefir using mass spectrometry-based omics approaches</strong></h5>
<ul>
<li>Haci Mehmet Kayili</li>
</ul>
<p>Kefir is an important food source based in the Caucasus region. The beneficial effects of kefir on human health are indicated in the literature. The mechanisms underlying these critical properties of kefir have not yet been elucidated. This study aims to examine the changes in milk protein profiles during kefir production and storage by using mass spectrometry-based-omics approaches. In addition, the detection of kefir-based proteins that transfer to milk from kefir microflora was investigated within the scope of the study. First, Kefir production was carried out. During the production and storage of kefir, kefir samples were taken from the periods determined in the study, and milk proteins were extracted. Peptides were produced using Lys-C and trypsin enzymes by a classical proteomics approach. Afterward, the fractionation of the peptides was carried out. Peptide-containing samples were analyzed by nLC-QExactive-Plus mass spectrometry. The data obtained as a result of the analysis were processed with the Maxquant software, and statistical analyzes were performed. According to the results obtained, significant changes were found in the milk proteome in 3 proteins between 0-12 hours, 39 proteins between 0-24 hours, 41 proteins between 0-7 days, and finally, 42 proteins between 0-28 days. From the 24th hour, a significant change was observed in 23 proteins. In the analysis, it was determined in the study that 398 kefir-based proteins were released into the milk. It is anticipated that these results will provide valuable contributions to the literature in terms of showing the changes in the milk proteome in kefir production.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Determination_of_changes_in_the_milk_proteome_in_production_and_storage_of_kefir_using_mass_spectrometry_based_omics_approaches_Haci_Mehmet_Kayili_866576d951/CSHS-Konference-CEEPC-IPM-CMSC-2022-Determination-of-changes-in-the-milk-proteome-in-production-and-storage-of-kefir-using-mass-spectrometry-based-omics-approaches-(Haci-Mehmet-Kayili).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Determination of changes in the milk proteome in production and storage of kefir using mass spectrometry-based omics approaches (Haci Mehmet Kayili)"/></p>
<h5><strong>12:20 - 12:40 Non-Immunoaffinity Extraction of Intact Proteins from Biological Fluids and Their Analysis by Liquid Chromatography – Triple Quadrupole Mass Spectrometry</strong></h5>
<ul>
<li>Katarína Maráková</li>
</ul>
<p>One of the crucial steps in quantitation of intact proteins from complex biological matrices is, except their reliable analysis, also sample preparation to achieve sufficient specificity and sensitivity. Commonly used immunoaffinity-based methods are characterized by their superior selectivity, although this can be a drawback if simultaneous analysis of multiple different proteins from a single sample is required. In our work, we developed non-immunoaffinity sample preparation based on a generally widely affordable microelution solid phase extraction for eleven model intact proteins (5.5 – 29 kDa) with various isoelectric points (4.5 - 11.3). Extracted intact proteins were analysed by reversed-phase liquid chromatography coupled with a triple quadrupole mass spectrometer operated in a multiple reaction monitoring (MRM) mode. Reversed-phase separations were performed on the Restek wide-pore Viva C4 column, as mobile phases served water and acetonitrile acidified by 0.1% difluoroacetic acid and 0.2% formic acid. The best recoveries for most of the selected proteins were obtained by using the HLB stationary phase. 1% trifluoroacetic acid and 0.2% Triton X-100 were used as efficient pretreatment reagents to release interactions between the proteins and biological matrix. Multiple sample loading was found out to be essential to obtain recoveries &gt;65% in urine for all targeted proteins (up to 30kDa) and &gt;50% in serum/plasma for most of the proteins. Limits of quantitation in biological matrices were in the range 2 - 1200 ng/mL, corresponding to 0.23 - 97.6 nM.</p>
<h5><strong>12:40 - 13:00 Advancing Cyclic Ion Mobility Mass Spectrometry Methods for Studying Biomolecules: Towards the Conformational Dynamics of Mega Dalton Protein Aggregates</strong></h5>
<ul>
<li>Adam Pruška</li>
</ul>
<p>Native mass spectrometry is a powerful tool for the analysis of non-covalent complexes. When coupled with high-resolution ion mobility, this technique can be used to investigate the conformational changes induced in said complexes by different solution or gas-phase conditions. In this study, we describe how a new generation high-resolution ion mobility instrument equipped with a cyclic ion mobility cell can be utilized for the analysis of large biomolecular systems, including temperature-induced protein aggregates of masses greater than 1.5 MDa, as well as a 63 kDa oligonucleotide complex. The effects of and the interplay between the voltages applied to the different components of the cyclic ion mobility spectrometry system on ion transmission and arrival time distribution were demonstrated using biomolecules covering the m/z range 2 000 to 10 000. These data were used to establish a theoretical framework for achieving the best separation on the cyclic ion mobility system. Finally, the cyclic ion mobility mass spectrometer was coupled with a temperature-controlled electrospray ionization source to investigate high-mass protein aggregation. This analysis showed that it was possible to continuously monitor the change in abundance for several conformations of MDa aggregates with increasing temperature. This work significantly increases the range of biomolecules that can be analyzed by both cyclic ion mobility and temperature-controlled electrospray ionization mass spectrometry, providing new possibilities for high-resolution ion mobility analysis.</p>
<h5><strong>13:00 - 15:30 Lunch + Poster session I (Odd-numbered posters)</strong></h5>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Posterova_sekce_I_Postery_liche_aecf40bb37/CSHS-Konference-CEEPC-IPM-CMSC-2022-Posterova-sekce-I-(Postery-liche).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Poster session I (Odd-numbered posters)"/></p>
<h4><strong>15:30 - 16:30 Company Workshop – Bruker Daltonics</strong></h4>
<h5><strong>The latest proteomics development at Bruker</strong></h5>
<ul>
<li>Gary Kruppa</li>
</ul>
<h5><strong>Clinical (phospho)proteomics with TIMS and PASEF</strong></h5>
<ul>
<li>Florian Meier</li>
</ul>
<h5><strong>16:30 - 16:50 Coffee break</strong></h5>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Petr_Novak_a_Michael_Volny_8288df35cd/CSHS-Konference-CEEPC-IPM-CMSC-2022-Petr-Novak-a-Michael-Volny.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Petr Novák and Michael Volný"/></p>
<h4><strong>16:50 - 17:30 Short Talks</strong></h4>
<ul>
<li>Chairperson: Petr Man</li>
</ul>
<h5><strong>Detection of C. difficile Toxin B by biomolecule-modified chips and mass spectrometry</strong></h5>
<ul>
<li>Josef Dvořák</li>
</ul>
<h5><strong>Optimized HDX-MS workflow for antibody structure monitoring by HDX-MS</strong></h5>
<ul>
<li>Zuzana Kalaninová</li>
</ul>
<h5><strong>RPLC-UV and HILIC-UV characterisation of on-site produced Ramucirumab-DTPA immunoconjugate</strong></h5>
<ul>
<li>Denis Naplekov</li>
</ul>
<h5><strong>Collision energy setting in proteomics and glycoproteomics: From individual species to a practical perspective</strong></h5>
<ul>
<li>Agnes Revesz</li>
</ul>
<h5><strong>Novel circulating biomarkers of biventricular heart failure</strong></h5>
<ul>
<li>Matěj Běhounek</li>
</ul>
<h5><strong>Comprehensive metabolomic and lipidomic study of tauopathy and Alzheimer&#39;s disease patients</strong></h5>
<ul>
<li>Dana Dobešová</li>
</ul>
<h5><strong>Overlooked oligomerization process in Azurin, a model metalloprotein</strong></h5>
<ul>
<li>Roman Tuzhilkin</li>
</ul>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Den_1_62de462e8b/CSHS-Konference-CEEPC-IPM-CMSC-2022-Den-1.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Day 1"/></p>
<h4><strong>17:30 - 18:00 Josef Chmelik Award</strong></h4>
<h5><strong>18:00 - 19:30 Guided tour to the conference dinner</strong></h5>
<h4><strong>19:30 - 23:00 Conference dinner and sightseeing boat trip</strong></h4>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Slavnostni_vecere_a_vylet_na_lodi_249c7c3702/CSHS-Konference-CEEPC-IPM-CMSC-2022-Slavnostni-vecere-a-vylet-na-lodi.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Conference dinner and sightseeing boat trip"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Slavnostni_vecere_a_vylet_na_lodi_3_6452afa91a/CSHS-Konference-CEEPC-IPM-CMSC-2022-Slavnostni-vecere-a-vylet-na-lodi-3.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Conference dinner and sightseeing boat trip"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Slavnostni_vecere_a_vylet_na_lodi_2_42bb7f554d/CSHS-Konference-CEEPC-IPM-CMSC-2022-Slavnostni-vecere-a-vylet-na-lodi-2.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022 Conference dinner and sightseeing boat trip"/></p>


Conference CEEPC/IPM/CMSC 2022 (Day 1)

<p><strong>In the  week from October 3, 2022, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p>
<blockquote>
<h4><a href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter</strong> in more than <strong>2 000 GC, LC nad MS webinars</strong></p>
</blockquote>
<h5><strong>1. Agilent Technologies: Improving UV-Vis Efficiency with Simultaneous Data Collection</strong></h5>
<ul>
<li>Mo, 3.10.2022, 5:00 (CEST)</li>
</ul>
<p>We will discuss in this webinar what to do in these scenarios - and how simultaneous data collection can speed up sample throughput and improve workflows.</p>
<h5><strong>2. Separation Science/MilliporeSigma: Carboxen® Synthetic Carbon: A Derivatized Resin for Process and Preparative Chromatography</strong></h5>
<ul>
<li>Tu, 4.10.2022, 11:00 (CEST)</li>
</ul>
<p>Webinar highlighting the advantages and value of synthetic carbon separation media in both liquid and gas purifications.</p>
<h5><strong>3. Thermo Fisher Scientific: Contaminants in food - Sample preparation and POPs</strong></h5>
<ul>
<li>Tu, 4.10.2022, 11:00 (CEST - Prague)</li>
</ul>
<p>Webinar will cover topics like Sample preparation in food analysis or Persistent organic pollutants analysis by GC-MS/MS.</p>
<h5><strong>4. SelectScience/Agilent Technologies/Thermo Fisher Scientific/Oxford Instruments/PAL Systems/RAFA: Virtual Food Analysis Summit 2022</strong></h5>
<ul>
<li>Tu, 4.10.2022, 13:30 (CEST)</li>
</ul>
<p>Join us online, in real-time, to discuss the very latest developments in food safety analysis, food legislation and quality control, food pathogens, pesticides, mycotoxins, and more.</p>
<h5><strong>5. Technology Networks/Agilent Technologies: Applied Versatility: Automated Liquid Handling for Many Applications</strong></h5>
<ul>
<li>Tu, 4.10.2022, 15:00 (CEST - Prague)</li>
</ul>
<p>In this webinar, we will highlight the uses and adaptations of the Agilent Bravo liquid handling platform.</p>
<h5><strong>6. Agilent Technologies: Tips for Developing Successful Solid Phase Extraction Methods</strong></h5>
<ul>
<li>Tu, 4.10.2022, 17:00 (CEST)</li>
</ul>
<p>In this talk, we will discuss tips for selecting, developing, and implementing simple and successful solid phase extraction methods.</p>
<h5><strong>7. Agilent Technologies: Multiplatform Untargeted Metabolomics (LC/MS, GC/MS, CE/MS) vs Semitargeted LC/MS for Lipids and Polar Compounds</strong></h5>
<p>-We, 5.10.2022, 11:00 (CEST)</p>
<p>Advantages and limitations of these analytical platforms will be discussed with some examples from our laboratory.</p>
<h5><strong>8. Agilent Technologies: Solutions and best practices for 2D-LC</strong></h5>
<ul>
<li>We, 5.10.2022, 17:00 (CEST)</li>
</ul>
<p>Get an overview of 2D-LC instrumentation for multi-heart cutting and comprehensive 2D-LC.</p>
<h5><strong>9. Agilent Technologies: Sweetening you Sugar Analysis with Electrichemical detection</strong></h5>
<ul>
<li>We, 5.10.2022, 19:00 (CEST)</li>
</ul>
<p>In this talk we compare three commonly used techniques for the analysis of sugars. We will demonstrate the LOD and LOQs of each detector for a series of standards and samples.</p>
<h5><strong>10. Agilent Technologies: Oligo to Go: Navigating your LC/MS Options for Oligonucleotide Characterization</strong></h5>
<ul>
<li>We, 5.10.2022, 20:00 (CEST)</li>
</ul>
<p>This presentation will focus on a range of LCMS options for the characterization of oligo samples and data analysis strategies behind those efforts.</p>
<h5><strong>11. Thermo Fisher Scientific: Contaminants in food - NIAS in packaging and PFAS</strong></h5>
<ul>
<li>Th, 6.10.2022, 11:00 (CEST - Prague)</li>
</ul>
<p>Topics: NIAS in packaging and PFAS, A GC- and LC-MS workflow for determining NIAS in food packaging.</p>
<h5><strong>12. Thermo Fisher Scientific: Analysis of battery electrolytes</strong></h5>
<ul>
<li>Th, 6.10.2022, 11:00 (CEST)</li>
</ul>
<p>Learn about analytical solutions for lithium-ion battery material and structural analysis. Learn how IC, GC-MS, ICP-OES, and SEM support battery value chain from raw materials to recycling.</p>
<h5><strong>13. Technology Networks/Tosoh Bioscience: Refine Size Exclusion Analysis of Proteins Using Multi-Angle Light Scattering and Mass Spectrometry</strong></h5>
<ul>
<li>Th, 6.10.2022, 15:00 (CEST)</li>
</ul>
<p>This webinar will explain the different detection methods and their results. Special emphasis will be placed on the higher requirements for size exclusion columns preceding advanced detection methods.</p>
<h5><strong>14. Phenomenex: What Cleaning a Column Can Teach About HPLC Basics</strong></h5>
<ul>
<li>Th, 6.10.2022, 19:00 (CEST)</li>
</ul>
<p>Topics: Confidently select the proper solvents with which to clean a particular column. Cleaning an HPLC column for common applications. The retention mechanisms that occur during each LC methodology.</p>
<h5><strong>15. Agilent Technologies: Deciding What Accessories You Need for a High End UV-Vis-NIR Instrument</strong></h5>
<ul>
<li>Th, 6.10.2022, 20:00 (CEST)</li>
</ul>
<p>An examination into the types of measurements and the accessory(s) needed to gather the desired data will be made in this discussion.</p>
<h5><strong>16. Agilent Technologies: Understanding UV-Vis (-NIR) Instrument Parameters</strong></h5>
<ul>
<li>Fr, 7.10.2022, 19:00 (CEST)</li>
</ul>
<p>While making a UV-Vis (-NIR) measurement is simple, making an accurate measurement requires an understanding of instrument parameters and their effects on the measurement.</p>


Webinars LabRulezLCMS Week 40/2022

Conference CEEPC/IPM/CMSC 2022 (Day 2)

<p><strong>DataApex is pleased to announce that a new control module for the <a href="https://lcms.labrulez.com/library?from=2020&amp;instrumentationItems=27&amp;mainauthorItems=1&amp;search=Nexera&amp;to=2022">Nexera LC-40 HPLC system</a> has been released in June 2022! It is a part of <a href="https://www.dataapex.com/product/controls-lc-shimadzu-nexera?language_content_entity=en">Clarity - Shimadzu Edition Chromatography Software</a> that is dedicated to the European market. Customers can use a control module for the Nexera LC-40 HPLC system in combination with hundreds of instruments supported in Clarity, including the <a href="https://gcms.labrulez.com/library?instrumentationItems=1&amp;mainauthorItems=1&amp;search=nexis+2030">Shimadzu Nexis GC-2030</a>.</strong></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/driven_by_clarity_1024_1_5c9d9d86ac/driven-by-clarity-1024-1.webp" alt="DataApex: Shimadzu Nexera LC-40 HPLC and Nexis GC-2030 systems controlled by Clarity Chromatography Software"/></p>
<p><a href="https://gcms.labrulez.com/library?mainauthorItems=95&amp;search=clarity"><strong>Clarity Chromatography Software</strong></a> has a strong position in the <strong>chromatography data systems</strong> market. Clarity is highly regarded for its <strong>intuitive</strong> approach, excellent <strong>performance</strong>, <strong>cost-effectiveness</strong>, and proficient <strong>technical support</strong>. Clarity can control <a href="https://www.dataapex.com/controls"><strong>900+ different instruments</strong></a> from one environment. Its wide range of data acquisition interfaces allows connection to virtually <strong>any chromatograph</strong>. Clarity supports various applications (<strong>PDA</strong>, <strong>MS</strong>, <strong>GPC</strong>, <strong>NGA</strong>, etc.).</p>
<blockquote>
<h5><a href="https://www.dataapex.com/product/clarity-shimadzu-edition-demo?language_content_entity=en&amp;promotion=MKT1625"><strong>Clarity - Shimadzu Edition DEMO version</strong></a></h5>
</blockquote>
<blockquote>
<ul>
<li><strong>Preview</strong> all Clarity - Shimadzu Edition functions</li>
<li>Experience the look and feel of <strong>working</strong> with Clarity - Shimadzu Edition</li>
<li><strong>Data acquisition</strong> simulated from a data file</li>
<li>Demo data for <strong>data processing</strong></li>
<li>All controlled instruments available in demo mode</li>
</ul>
</blockquote>
<p><strong>DataApex</strong> is solely focused on <strong>chromatography software development</strong>. A strong emphasis is placed on technological innovation, visionary adoption of new laboratory standards, best practices, and extensive customer support. DataApex products are sold in <strong>over 100 countries</strong> around the world. Eleven chromatography instrument manufacturers resell privately labeled versions (<strong>OEMs</strong>) of <strong>DataApex’s software</strong>.</p>
<blockquote>
<p><strong>To obtain further information about DataApex Clarity products, please contact us at sales@dataapex.com or visit <a href="https://www.dataapex.com/">www.dataapex.com</a></strong></p>
</blockquote>


Shimadzu Nexera LC-40 HPLC system supported in Clarity

<p><strong>In the  week from October 10, 2022, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p>
<blockquote>
<h4><a href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter</strong> in more than <strong>2 000 GC, LC nad MS webinars</strong></p>
</blockquote>
<h5><strong>1. Agilent Technologies: Moving from R&amp;D and Limited QC on a UV-Vis-NIR instrument to Large Scale QC Using an Autosampler</strong></h5>
<ul>
<li>Mo, 10.10.2022, 08:30 (CEST)</li>
</ul>
<p>During this presentation, we will look at the aspects of adding and using an autosampler to Maximize QC Workflow.</p>
<h5><strong>2. Separation Science: Biphenyl, the Go To Phase for LC-MS Method Development</strong></h5>
<ul>
<li>Tu, 11.10.2022, 11:00 (CEST)</li>
</ul>
<p>The webinar describing how the unique nature of Biphenyl imparts selectivity to isobaric compounds that is not readily achievable by other phases with simple, friendly and LC-MS amenable solvent systems.</p>
<h5><strong>3. Thermo Fisher Scientific: Back-to-school: The real impact of HPLC and column parameters</strong></h5>
<ul>
<li>Tu, 11.10.2022, 18:00 (CEST)</li>
</ul>
<p>In this educational back-to-school webinar we will discuss strategic approaches for development, optimization and transfer of legacy and modern HPLC methods, utilizing gradient elution.</p>
<h5><strong>4. Phenomenex: Improvements in Sample Preparation and LC-MS Analysis of Therapeutic Oligonucleotides</strong></h5>
<ul>
<li>Tu, 11.10.2022, 19:00 (CEST)</li>
</ul>
<p>Topics: Take in a brief introduction to oligonucleotide diversity. Learn about detailed resolution of oligonucleotides. Find new column-based, and method-based, oligo analysis tool approaches</p>
<h5><strong>5. Separation Science: Critical Insights in the Detection of PFAS in Our Environment: How the PerkinElmer QSight LC/MS/MS System Can Offer a Robust and Reliable Solution</strong></h5>
<ul>
<li>We, 12.10.2022, 11:00 (CEST)</li>
</ul>
<p>Presentation will explore some high-performance solutions to optimize your laboratory workflow for PFAS analysis.</p>
<h5><strong>6. Agilent Technologies: Determination of Per and Polyfluoroalkyl Substances in Soils Using Carbon S SPE by LC/MS/MS</strong></h5>
<ul>
<li>We, 12.10.2022, 19:00 (CEST)</li>
</ul>
<p>This study investigates the post-extraction matrix cleanup of 59 PFAS from loamy sand, reed sedge peat, and topsoil using the Bond Elut Carbon S 250 mg, 6 mL cartridges followed by LC/MS/MS analysis.</p>
<h5><strong>7. Agilent Technologies: Ultra-High Sensitivity for Targeted Proteomic using Evosep-MRM at Single Cell Level</strong></h5>
<ul>
<li>We, 12.10.2022, 20:00 (CEST)</li>
</ul>
<p>The robustness and throughput of the Whisper methods on the Evosep One combined with the sensitivity of the Agilent 6495C triple quadrupole support targeted analysis of proteomes at the single cell level.</p>
<h5><strong>8. Phenomenex: Pirates of Chromatography: Tales of Sharp Peaks</strong></h5>
<ul>
<li>Th, 13.10.2022, 05:00 (CEST)</li>
</ul>
<p>Topics: Signal to noise improvement, Unknown/Ghost/Carryover Peaks, No Peaks, Split peaks/Fronting peaks/Double peaks, Negative peaks, Peak Sequence change, Irreproducible peak heights and peak areas.</p>
<h5><strong>9. Thermo Fisher Scientific: Proteomics as a biological assay through sample multiplexing</strong></h5>
<ul>
<li>Th, 13.10.2022, 17:00 (CEST)</li>
</ul>
<p>This webcast will describe utilizing a modified Thermo Scientific™ Orbitrap™ Tribrid™ mass spectrometer system to evaluate multiplexed whole-proteomes coverage as a biological assay for drug discovery.</p>
<h5><strong>10. Waters Corporation: Introduction to Multidimensional Polymer-LC</strong></h5>
<ul>
<li>Th, 13.10.2022, 17:00 (CEST)</li>
</ul>
<p>The presentation will introduce the concept of 2-dimensional chromatography, the instrumental setup as well as typical applications.</p>


Webinars LabRulezLCMS Week 41/2022

<h3><strong>Program CEEPC/IPM/CMSC 2022</strong></h3>
<blockquote>
<p><a href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/abstractbook_2022_3bec805e4e/abstractbook_2022.pdf"><strong>Complete program including abstracts for download</strong></a></p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_2_aa28bf7c17/CSHS-Konference-CEEPC-IPM-CMSC-2022-2.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022"/></p>
<h3><strong>Saturday October 1, 2022</strong></h3>
<h4><strong>8:50 - 10:50 Session V – Applications in Biomedicine 2</strong></h4>
<ul>
<li>Chairperson: Haci Mehmet Kayili</li>
</ul>
<h5><strong>8:50-9:10 Proteomic Analysis of Hepatitis B Virus</strong></h5>
<ul>
<li>Martin Hubálek</li>
</ul>
<p>According to the World Health Organisation, an estimated 296 million people worldwide are infected with Hepatitis B virus (HBV). HBV is a small enveloped DNA virus from Hepadnaviridae family that persists in the infected hepatocytes by establishing an episomal covalently closed circular double-stranded DNA (cccDNA) genome containing four open reading frames (C, P, S and X). The ORFs largely overlap and encode multiple proteins using different in-frame start codons. The S ORF encodes three forms of surface envelope glycoproteins S-, M-, and L- HBs). The HBV preC-C gene gives rise to two different products translated from distinct mRNAs – core protein (HBc) and precore protein (HBe). Despite their high sequence similarity, these proteins exhibit different functions and subcellular localizations. The P ORF encodes viral polymerase, and the X ORF encodes regulatory HBx protein.</p>
<p>Mass Spectrometry department of IOCB has established a collaboration with the groups of Iva Pichova and Jan Weber from IOCB. This collaboration on proteomics experiments lead to the discovery of several important biological and biochemical findings including identification of interacting partners of HBc, HBx and HBe proteins or posttranslational modifications of HBc protein. The collaboration resulted in several publications. The proteomic analysis of individually expressed viral proteins has also captured out-of-frame products suggesting the possibility of frameshift or alternative internal initiation of translation. The presentation will highlight the proteomic analyses of HBV at IOCB</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Proteomic_Analysis_of_Hepatitis_B_Virus_Martin_Hubalek_a95dd6216f/CSHS-Konference-CEEPC-IPM-CMSC-2022-Proteomic-Analysis-of-Hepatitis-B-Virus-(Martin-Hubalek).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Proteomic Analysis of Hepatitis B Virus (Martin Hubálek)"/></p>
<h5><strong>9:10-9:30 The single amino acid substitutions in Mason-Pfizer Monkey Virus matrix protein modulate its proteolytic cleavage rate</strong></h5>
<ul>
<li>Jakub Sýs</li>
</ul>
<p>N-terminal domain of polyprotein Gag of Mason-Pfizer Monkey Virus (M-PMV) Matrix protein (MA) is naturally myristoylated on its N-terminus. When MA is a part of polyprotein Gag, the myristate moiety is buried inside hydrophobic pocket and exposed probably upon interaction with host cell plasma membrane (PM). This mechanism called myristoyl switch does not occur in M-PMV as readily as it does in HIV-1, suggesting that it may have an important role in M-PMV maturation by regulating the MA cleavage from Gag polyprotein.</p>
<p>To address this hypothesis, we have treated the myristoylated (myrMAPPHis) and nonmyristoylated MAPPHis (nonmyrMAPPHis) M PMV construct, bearing the cleavage site for M-PMV viral protease (Pr13), on artificial liposomes mimicking PM by Pr13 for several time points. In contrast to rapidly degraded nonmyrMAPPHis even without liposomes, the myrMAPPHis, surprisingly, become cleaved more frequently only after addition of liposomes indicating the possible exposure of myristate upon interaction with PM.</p>
<p>To support our findings, we have designed and examined also four mutants of MAPPHis M PMV with single amino acid substitutions with expectation to block or, conversely, facilitate the myristoyl switch by stabilization (A79V, A79L) or destabilization (I51A, I86A) of MA M PMV hydrophobic pocket. The mutants A79V and A79L were cleaved even less effectively than myrMAPPHis as well as the degradation of mutants I51A and I86A was faster compared to nonmyrMAPPHis. The different cleavage rates of proteins were confirmed also on structural level by using method of hydrogendeuterium exchange coupled with mass spectrometric detection. It shows that the protease cleavage site has different dynamics in proteins as a result of disruption/stabilisation of hydrophobic pocket.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_The_single_amino_acid_substitutions_in_Mason_Pfizer_Monkey_Virus_matrix_protein_modulate_its_proteolytic_cleavage_rate_Jakub_Sys_e300792938/CSHS-Konference-CEEPC-IPM-CMSC-2022-The-single-amino-acid-substitutions-in-Mason-Pfizer-Monkey-Virus-matrix-protein-modulate-its-proteolytic-cleavage-rate-(Jakub-Sys).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: The single amino acid substitutions in Mason-Pfizer Monkey Virus matrix protein modulate its proteolytic cleavage rate (Jakub Sýs)"/></p>
<h5><strong>9:30-9:50 Cardiac alarmins as residual risk markers of atherosclerosis under lipid-lowering therapy</strong></h5>
<ul>
<li>Viorel-Iulian Suica</li>
</ul>
<p>Background: The major cause in the initiation and progression of atherosclerosis are the high levels of low-density lipoproteins. Although statin treatment can effectively lower these levels, there is still a residual risk of cardiovascular events. We hypothesize that a specific panel of alarmins, a family of stress-sensing molecules, could indicate the persistence of silent atherosclerosis residual risk. </p>
<p>Methods: New Zeeland White rabbits were divided into: the control group (C) with standard diet, a group which received a high-fat diet for 12 weeks (Au) and a treated hyperlipidemic group, with a lipid diet for 8 weeks followed by standard diet and hypolipidemic treatment (atorvastatin and PCSK9 siRNA-inhibitor) for 4 weeks (Asi). We used the LTQ Orbitrap Velos Pro mass spectrometer to analyse the left ventricle lysates. The experiments were complemented by immunologic and genomic assays to corroborate the data.</p>
<p>Results: The hyperlipidemic diet determined a general alarmin up-regulation tendency over C group. A significant spectral abundance increase was measured for specific heat shock proteins, S100 family members, HMGB1 and Annexin A1. The hypolipidemic treatment demonstrated a reversed regulation trend, with non-significant spectral alteration over the C group for some identified alarmins.</p>
<p>Conclusion: Our study highlights the discriminating potential of alarmins in hyperlipidemia or following a hypolipidemic treatment.</p>
<h5><strong>9:50-10:10 Cervical mucus – a non-invasive uterine biomarker source?</strong></h5>
<ul>
<li>Tomáš Oždian</li>
</ul>
<p>Cervical mucus (CM) is a viscous fluid produced by cervical glands located in the myometrium of the uterine cervix. Usually, CM forms a plug between vagina and uterus. During the ovulation, CM starts to be more fluidic to allow the sperm to go through. During the ovulation, there is a good window for non-invasive sampling of the CM, originating from uterine derived tissues. The main hypothesis for CM proteomic study is to develop a method for CM sampling, processing and to answer if there are enough proteins in the CM for potential biomarker search. The resulting protocol consists of mucus dissolution, multi-enzyme digestion, and LC-MS proteomic analysis. The optimized approach allows to identify more than thousand proteins from every sample tested. The bioinformatic analysis reveals that the identified proteins are both intra and extra cellular and discovers the proteins with enriched expression in the broader female reproductive tract. To our best knowledge, we provide the most extensive proteomic characterization available.</p>
<h5><strong>10:10-10:30 High-Throughput µLC-MS/MS Lipidomics of 3D In Vitro Disease Models to Investigate Lipid Dysregulation</strong></h5>
<ul>
<li>Darshak Gadara</li>
</ul>
<p>Microflow liquid chromatography interfaced with mass spectrometry is increasingly applied for the high-throughput profiling of biological samples, as it demonstrated an acceptable tradeoff between sensitivity and reproducibility. However, a limited number of applications developed in lipidomics. This study introduces a robust, sensitive, and high-throughput μLC-MS/MS lipidomics workflow, applied in a differential lipidome study of APOE 3 and 4 phenotypes in iPSC-derived cerebral organoids (CO). First, we optimized the microbore column gradient conditions, injecting a mixture of 15 lipid standards on the UHPLC system coupled to a 6469 QQQ. Our μLC-MS/MS method allows quantitation of 351 lipid species from the single CO. The microflow lipidomics shows an average 3.7-fold increase in response compared to a conventional high-flow method. To benchmark the quantitative performance, 303 samples were acquired over 75 hours. The median coefficient of variation (CV) of 351 lipid species was 12.95%, only 26 lipid species show CV higher than 30%. We demonstrated that microflow separation coupled to three-stage quadruple MS substantially increases the sensitivity and allows for robust multiplexed lipid profiling (including cholesterol determination) in a single cerebral organoid. As a proof of concept, lipidomics analysis was performed for the lipid extract of APOE 3/3 and APOE 4/4 (n=10) CO samples. To our knowledge, this is the first systematic report to demonstrate the microflow LC-MS/MS allows for sensitive, high-throughput, and robust measurement of global lipidome, opening an avenue for routine application of microflow separation in the field of lipidomics.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_High_Throughput_LC_MS_MS_Lipidomics_of_3_D_In_Vitro_Disease_Models_to_Investigate_Lipid_Dysregulation_Darshak_Gadara_0935fe540d/CSHS-Konference-CEEPC-IPM-CMSC-2022-High-Throughput-LC-MS-MS-Lipidomics-of-3D-In-Vitro-Disease-Models-to-Investigate-Lipid-Dysregulation-(Darshak-Gadara).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: High-Throughput µLC-MS-MS Lipidomics of 3D In Vitro Disease Models to Investigate Lipid Dysregulation (Darshak Gadara)"/></p>
<h5><strong>10:30-10:50 Multi-omics network analysis in atherosclerosis reveals mechanisms driving the progression towords complicated lesion</strong></h5>
<ul>
<li>Eva Csosz</li>
</ul>
<p>From a biological point of view, it is crucial to understand the mechanisms laying behind atherosclerosis and its complicated forms. In the process of atherosclerosis, first, an atheromatous plaque builds up, which in some conditions can lead to the appearance of complicated lesions. The complicated lesion is characterized by ruptures on the atherosclerotic plaque surface and/or hemorrhage into the plaque. According to the WHO, hemorrhaged lesions, contribute to a considerable extent of deaths worldwide.</p>
<p>The main goal of our project was to perform a multi-omics study involving transcriptomics and proteomics data to get more insights into the pathophysiological processes dominating the complicated lesions.</p>
<p>Transcriptomic (RNAseq) and proteomic (label-free quantification) data were obtained by the analysis of artery samples originating from healthy arteries, atheromatous lesions, and complicated lesions. Both data types were examined separately by Ingenuity Pathway Analysis and later an overlay of the networks was achieved.</p>
<p>120 differentially expressed (DE) proteins and more than 4000 DE genes were detected. The deep analysis of the data could identify the proteins and transcripts characteristic of the complicated lesions and could reveal the pathways specific to atheroma and complicated lesions, respectively. The DE genes were arranged into 25 networks, while the DE proteins were into 12 networks. The lower number of DE proteins compared to the number of DE genes did not result in loss of information, indicating the power of the proteomics experiments. In spite of providing a lower number of DE items, the biological information achievable from proteomics experiments has equal value compared to transcriptomics data.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Multi_omics_network_analysis_in_atherosclerosis_reveals_mechanisms_driving_the_progression_towords_complicated_lesion_Eva_Csosz_aa54a2b04f/CSHS-Konference-CEEPC-IPM-CMSC-2022-Multi-omics-network-analysis-in-atherosclerosis-reveals-mechanisms-driving-the-progression-towords-complicated-lesion-(Eva-Csosz).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Multi-omics network analysis in atherosclerosis reveals mechanisms driving the progression towords complicated lesion (Eva Csosz)"/></p>
<h5><strong>10:50 - 11:10 Coffee break</strong></h5>
<h4><strong>11:10 - 12:00 Plenary lecture III:: Light-mediated discovery of surfaceome nanoscale organization and inter-cellular receptor interaction networks</strong> (Chairperson: Pavel Bouchal)</h4>
<ul>
<li>Bernd Wollscheid</li>
</ul>
<p>The molecular nanoscale organization of the surfaceome is a fundamental regulator of cellular signaling in health and disease. Technologies for mapping the spatial relationships of cell surface receptors and their extracellular signaling synapses would unlock theranostic opportunities to target protein communities and the possibility to engineer extracellular signaling. Here, we develop an optoproteomic technology termed LUX-MS that enables the targeted elucidation of acute protein interactions on and in between living cells using light-controlled singlet oxygen generators (SOG). By using SOG-coupled antibodies, small molecule drugs, biologics and intact viral particles, we demonstrate the ability of LUX-MS to decode ligand receptor interactions across organisms and to discover surfaceome receptor nanoscale organization with direct implications for drug action. Furthermore, by coupling SOG to antigens we achieved light-controlled molecular mapping of intercellular signaling within functional immune synapses between antigen-presenting cells and CD8⁺ T cells providing insights into T cell activation with spatiotemporal specificity. LUX-MS based decoding of surfaceome signaling architectures thereby provides a molecular framework for the rational development of theranostic strategies.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Light_mediated_discovery_of_surfaceome_nanoscale_organization_and_inter_cellular_receptor_interaction_networks_Bernd_Wollscheid_019214ab5c/CSHS-Konference-CEEPC-IPM-CMSC-2022-Light-mediated-discovery-of-surfaceome-nanoscale-organization-and-inter-cellular-receptor-interaction-networks-(Bernd-Wollscheid).webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Light-mediated discovery of surfaceome nanoscale organization and inter-cellular receptor interaction networks (Bernd Wollscheid)"/></p>
<h4><strong>12:00 - 12:20 Poster prize, Closing remarks</strong></h4>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Predavani_oceneni_konference_CEEPC_IPM_CMSC_2022_Ales_Kvasnicka_a_Michael_Volny_4c0967dd3b/CSHS-Predavani-oceneni-konference-CEEPC-IPM-CMSC-2022-(Ales-Kvasnicka-a-Michael-Volny).webp" alt="ČSHS: Conference prize CEEPC/IPM/CMSC 2022 (Aleš Kvasnička a Michael Volný)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Eu_PA_cestovni_granty_Nejlepsi_kratke_sdeleni_Kalaninova_Zuzana_a_Eva_Csosz_ef6a262eae/CSHS-EuPA-cestovni-granty-Nejlepsi-kratke-sdeleni-(Kalaninova-Zuzana-a-Eva-Csosz).webp" alt="ČSHS: EuPA travel grants: Short talk prize CEEPC/IPM/CMSC 2022 (Kalaninová Zuzana a Eva Csosz)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Vyhlaseni_nejlepsiho_posteru_Konference_CEEPC_IPM_CMSC_2022_Anton_Skriba_a_Michael_Volny_0010fccaaa/CSHS-Vyhlaseni-nejlepsiho-posteru-Konference-CEEPC-IPM-CMSC-2022-(Anton-Skriba-a-Michael-Volny).webp" alt="ČSHS: Poster prize CEEPC/IPM/CMSC 2022 (Anton Škríba a Michael Volný)"/></p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Eu_PA_cestovni_granty_Nejlepsi_poster_Jadeja_Siddharth_a_Eva_Csosz_dac2e7c761/CSHS-EuPA-cestovni-granty-Nejlepsi-poster-(Jadeja-Siddharth-a-Eva-Csosz).webp" alt="ČSHS: EuPA travel grants: Poster prize CEEPC/IPM/CMSC 2022 (Jadeja Siddharth a Eva Csosz)"/></p>
<h5><strong>12:20 - 13:30 Lunch</strong></h5>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/CSHS_Konference_CEEPC_IPM_CMSC_2022_Obcerstveni_44ad451fcc/CSHS-Konference-CEEPC-IPM-CMSC-2022-Obcerstveni.webp" alt="CSMS: Conference CEEPC/IPM/CMSC 2022: Coffe break"/></p>


Conference CEEPC/IPM/CMSC 2022 (Day 3)

<p><strong>STARSS project is approaching its end after more than five years. A few more months remain before it will be concluded. The major objective of the project was to create a strong team of researchers enabling significant progress in the field of the separation science. The annual STARSS conferences have always been a good opportunity to bring people from several Czech and European institutions in Hradec Kralove where we have met face to face for a few days in order to inform each other about the progress in the research. It is clear that the focus of these conferences has been on the exchange in scientific information. However, an integral part of our previous conferences has also included informal meetings when we had time to talk about issues sometimes even completely outside of science perhaps with a drink and food. Social part of conferences is simply indispensable.</strong></p>
<p><strong>I trust that this conference will be at least as interesting as all the previous ones. Its program is rich and includes presentations by renowned “icons” of separation science from both abroad and the Czech Republic, as well as talks given by students and postdocs. Such “cross-pollination” will certainly be uplifting for all involved. I wish all those who attend this conference a pleasant stay in Hradec Králové and enjoyable conferencing.</strong></p>
<p><strong><a href="https://lcms.cz/articles?search=Franti%C5%A1ek+%C5%A0vec">Prof. František Švec</a></strong></p>
<h3><strong>Program: 6th STARSS Conference on Separation Science</strong></h3>
<ul>
<li><a href="https://portal.faf.cuni.cz/getattachment/Projects/STARSS/Events/6th-STARSS-conference/Program/2022-STARSS-booklet.pdf.aspx?lang=en-GB"><strong>Conference booklet including speakers bio</strong></a></li>
</ul>
<h3><strong>DAY 1: Monday 17. 10. 2022</strong></h3>
<h5><strong>08:00 – 09:00 REGISTRATION</strong></h5>
<h5><strong>09:00 – 09:20 Welcoming address</strong></h5>
<ul>
<li>Prof. Petr Solich, Prof. František Švec, Prof. Lucie Nováková</li>
<li>Assoc. Prof. Jaroslav Roh (the dean of the Faculty of Pharmacy, Faculty of Pharmacy, Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h4><strong>SESSION I: IMPORTANCE OF SEPARATION SCIENCE FOR CLINICAL APPLICATIONS</strong></h4>
<ul>
<li>Chairperson: Prof. František Švec</li>
</ul>
<h5><strong>09:20 – 10:00 L-01: UHPSFC/MS as a powerful tool for high-throughput lipidomic quantitation in cancer screening</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Michal+Hol%C4%8Dapek">Prof. Michal Holčapek</a> (University of Pardubice, Czech Republic)</li>
</ul>
<h5><strong>10:00 – 10:40 L-02: Selected ion flow tube mass spectrometry, SIFT-MS, analyses of volatile metabolites in exhaled breath</strong></h5>
<ul>
<li>Prof. Patrik Španěl (J. Heyrovský Institute of Physical Chemistry, Prague, Czech Republic)</li>
</ul>
<h5><strong>10:40 – 11:10 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION II: BIOPHARMACEUTICALS</strong></h4>
<ul>
<li>Chairperson: Prof. Lucie Nováková</li>
</ul>
<h5><strong>11:10 – 11:50 L-03: Simplifying the characterization of complex biopharmaceutical products through the use of ultra-short columns or multi-isocratic elution mode</strong></h5>
<ul>
<li>Dr. Davy Guillarme (University of Geneva, Switzerland)</li>
</ul>
<h5><strong>11:50 – 12:20 L-04: Analysis of therapeutic proteins by multidimensional LC: How far can we go?</strong></h5>
<ul>
<li>Dr. Valentina D ́Atri (University of Geneva, Switzerland)</li>
</ul>
<h5><strong>12:20 – 12:40 L-05: pyroLC: online thermal decomposition of proteins as a tool for ultra-fast bottom-up proteomics</strong></h5>
<ul>
<li>Dr. Juraj Lenčo (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>12:40 – 13:00 L-06: RPLC-UV and HILIC-UV characterization of home-made ramucirumab-based antibody-drug conjugate</strong></h5>
<ul>
<li>Denis Naplekov (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>13:00 – 14:00 Lunch</strong></h5>
<h4><strong>SESSION III: 2D SEPARATIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Jiří Urban</li>
</ul>
<h5><strong>14:00 – 14:40 L-07: What can we expect from LC x LC compared to conventional 1D-LC</strong></h5>
<ul>
<li>Prof. Sabine Heinisch (Université Claude Bernard Lyon 1, Lyon, France)</li>
</ul>
<h5><strong>14:40 – 15:20 L-08: Modelling of analyte profiles and band broadening generated by interface loops used in multi-dimensional liquid chromatography</strong></h5>
<ul>
<li>Prof. Ken Broeckhoven (Vrije Universiteit Brussel, Belgium)</li>
</ul>
<h5><strong>15:20 – 15:50 L-09: Off-line LC x SFC in the prospect of upgrading natural or recycled products</strong></h5>
<ul>
<li>Dr. Karine Faure (Université Claude Bernard Lyon 1, Lyon, France)</li>
</ul>
<h5><strong>15:50 – 16:20 L-10: Two-dimensional liquid chromatographic separations of complex samples of industrial products and intermediates</strong></h5>
<ul>
<li><a href="https://lcms.cz/articles?search=Petr+%C4%8Cesla"><strong>Assoc. Prof. Petr Česla</strong></a> (University of Pardubice, Czech Republic)</li>
</ul>
<h5><strong>16:20 – 16:50 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION IV: IMPORTANCE OF STEROIDS</strong></h4>
<ul>
<li>Chairperson: Prof. Marcela Segundo</li>
</ul>
<h5><strong>16:50 – 17:30 L-11: An image of the future of steroid biochemistry</strong></h5>
<ul>
<li>Prof. Ruth Andrew (University of Edinburgh, UK)</li>
</ul>
<h5><strong>17:30 – 18:00 L-12: Ecdysterone metabolism: A holistic view on compound identification for doping control</strong></h5>
<ul>
<li>Prof. Maria Kristina Parr (Freie Universität Berlin, Berlin, Germany)</li>
</ul>
<h5><strong>18:00 – 18:30 L-13: New liquid chromatography-tandem mass spectrometry method for targeted profiling of steroids in mouse plasma</strong></h5>
<ul>
<li>Taťána Gazárková (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>18:30 – 19:30 Welcome drink</strong></h5>
<h3><strong>DAY 2: Tuesday 18. 10. 2022</strong></h3>
<h5><strong>08:00 – 09:00 REGISTRATION</strong></h5>
<h4><strong>SESSION V: SUPERCRITICAL FLUID CHROMATOGRAPHY AND EXTRACTIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Radim Kučera</li>
</ul>
<h5><strong>09:00 – 09:30 L-14: Supercritical fluid extraction and chromatography in life science applications</strong></h5>
<ul>
<li>Prof. Charlotta Turner (Lund University, Lund, Sweden)</li>
</ul>
<h5><strong>09:30 – 10:00 L-15: Supercritical fluid chromatography – tandem mass spectrometry for analysis of vitamin D metabolites in biological samples</strong></h5>
<ul>
<li>Dr. Margareta Sandahl (Lund University, Lund, Sweden)</li>
</ul>
<h5><strong>10:00 – 10:30 L-16: The effect of make-up solvent composition on responses in SFC-MS: Electrospray vs UniSpray</strong></h5>
<ul>
<li>Dr. Kateřina Plachká (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>10:30 – 11:00 L-17: UHPSFC-MS/MS versus 2D-UHPLC-MS/MS in chiral separation of Propranolol and its Hydroxy Metabolites</strong></h5>
<ul>
<li>Lukas Harps (Freie Universität Berlin, Berlin, Germany)</li>
</ul>
<h5><strong>11:00 – 11:30 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION VI: COLUMN TECHNOLOGY</strong></h4>
<ul>
<li>Chairperson: Prof. Dalibor Šatínský</li>
</ul>
<h5><strong>11:30 – 12:10 L-18: Hypercrosslinked monolithic stationary phases in proteomics</strong></h5>
<ul>
<li>Assoc. Prof. Jiří Urban (Masaryk University, Brno, Czech Republic)</li>
</ul>
<h5><strong>12:10 – 12:40 L-19: Guidelines for tuning the macropore structure of monolithic columns for high-performance liquid chromatography</strong></h5>
<ul>
<li>Dr. Sebastiaan Eeltink (Vrije Universiteit Brussel, Belgium)</li>
</ul>
<h5><strong>12:40 – 13:10 L-20: Strategies and chemistry in column development</strong></h5>
<ul>
<li>Assoc. Prof. Michal Kohout (University of Chemistry and Technology, Prague, Czech Republic)</li>
</ul>
<h5><strong>13:10 – 14:30 Lunch</strong></h5>
<h4><strong>SESSION VII: RETENTION MECHANISMS AND STATIONARY PHASES</strong></h4>
<ul>
<li>Chairperson: Prof. Spas Kolev</li>
</ul>
<h5><strong>14:30 – 15:00 L-21: Understanding the mechanism, advantages and limitations of hydrophilic interaction liquid chromatography</strong></h5>
<ul>
<li>Prof. David McCalley (University of West England, UK)</li>
</ul>
<h5><strong>15:00 – 15:30 L-22: Study of molecular structures through retention mechanisms in liquid chromatography: New trends and applications</strong></h5>
<ul>
<li>Dr. Jean-Christophe Garrigues (Paul Sabatier University - Toulouse III, Toulouse, France)</li>
</ul>
<h5><strong>15:30 – 16:00 L-23: Problems of stationary phases in SFC</strong></h5>
<ul>
<li>Prof. Lucie Nováková (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>16:00 – 16:30 L-24: The chromatographic behaviour of pharmaceuticals on stationary phases based on metal oxides</strong></h5>
<ul>
<li>Assoc. Prof. Radim Kučera (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>16:30 – 17:00 Coffee-break and discussion</strong></h5>
<h4><strong>SESSION VIII: SAMPLE PREPARATION AND APPLICATIONS</strong></h4>
<ul>
<li>Chairperson: Assoc. Prof. Hana Sklenářová</li>
</ul>
<h5><strong>17:00 – 17:30 L-25: Glucosinolate profiling in Brassicaceae family: instrumental analysis and biological application</strong></h5>
<ul>
<li>Dr. Iva Pavlovic (Palacký University, Olomouc, Czech Republic)</li>
</ul>
<h5><strong>17:30 – 18:00 L-26: Flow programming for the development of online solid-phase extraction</strong></h5>
<ul>
<li>Assoc. Prof. Petr Chocholouš (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>18:00 – 18:30 L-27: Automated tandem homogeneous liquid-liquid extraction and centrifugation-less deproteination for complex sample analysis on HPLC</strong></h5>
<ul>
<li>Dr. Burkhard Horstkotte (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>19:00 – 22:00 Conference dinner</strong></h5>
<h3><strong>DAY 3: Wednesday 19. 10. 2022</strong></h3>
<h4><strong>WORKSHOPS: FACULTY OF PHARMACY</strong></h4>
<h5><strong>08:00 – 10:30 W1: Supercritical fluid extraction of polar and nonpolar compounds from Eucalyptus sp.</strong></h5>
<ul>
<li>Laboratory 916, 8th floor, Faculty of Pharmacy</li>
<li>Dr. Veronika Pilařová (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>08:00 – 10:30 W2: MetAmino® Kit – simple and easy sample preparation method for your LC-MC or GC-MS analysis</strong></h5>
<ul>
<li>Student’s Laboratory, 3rd floor, Faculty of Pharmacy</li>
<li>Ing. Jana Volková (Chromservis, Czech Republic)</li>
</ul>
<h5><strong>10:30 – 11:00 Coffee-break and discussion</strong></h5>
<h5><strong>11:00 – 13:30 W3: Who will help you with sample prep and liquid handling?</strong></h5>
<ul>
<li>Student’s Laboratory, 4th floor, Faculty of Pharmacy</li>
<li>Dr. Martina Riesová (<a href="https://lcms.cz/companies/50"><strong>Waters</strong></a>, Prague, Czech Republic)</li>
</ul>
<h5><strong>13:30 – 14:30 Lunch</strong></h5>
<h5><strong>14:30 – 17:00 W4: Practical application of nanofibers for advanced extraction approaches in chromatography</strong></h5>
<ul>
<li>Student’s Laboratory no. 524, 4th floor, Faculty of Pharmacy</li>
<li>Prof. Dalibor Šatínský, Dr. Ivona Lhotská (Charles University, Hradec Králové, Czech Republic)</li>
</ul>
<h5><strong>17:00 – 18:00 Coffee-break and discussion</strong></h5>


6th STARSS Conference on Separation Science (Program)

<p><strong>In the  week from October 17, 2022, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p>
<blockquote>
<h4><a href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4>
</blockquote>
<blockquote>
<p><strong>👉 Search and filter</strong> in more than <strong>2 000 GC, LC nad MS webinars</strong></p>
</blockquote>
<h5><strong>1. Merck: Strategies for Efficient Analyses of Proteins: From Aggregates to Post-Translational Modifications</strong></h5>
<ul>
<li>Tu, 18.10.2022, 15:00 (CEST)</li>
</ul>
<p>Strategies and techniques to maximize efficiency and resolution when using reversed phase chromatography (RPC) and size exclusion chromatography (SEC) in analyzing biomacromolecules.</p>
<h5><strong>2. Waters Corporation: Desorption Electrospray Ionization (DESI) MS – A powerful tool for chemical and materials surface analysis</strong></h5>
<ul>
<li>Tu, 18.10.2022, 17:00 (CEST)</li>
</ul>
<p>We will take a look at some materials analysis applications developed within waters and highlight technology improvements of the new DESI XS source.</p>
<h5><strong>3. Separation Science: Glycan Analysis in Biopharma: Challenges &amp; Future Directions</strong></h5>
<ul>
<li>We, 19.10.2022, 16:00 (CEST)</li>
</ul>
<p>An expert presentation and discussion event in which experts discuss their approaches to the challenges of glycosylation analysis, focused on biotherapeutics and biomarkers.</p>
<h5><strong>4. Agilent Technologies: Implementation of 2 - Dimensional Chromatography for Characterization of Non - Ionic Surfactants with Accurate Mass Time of Flight Spectrometry</strong></h5>
<ul>
<li>We, 19.10.2022, 19:00 (CEST)</li>
</ul>
<p>In the first dimension the separation is by degree of ethoxylation, while in the second dimension by the separation occurs by the length of the hydrophobic tail.</p>
<h5><strong>5. Agilent Technologies: Targeted Proteomic Analysis of Cell Death Pathway Activation in Cancer Cell Lines</strong></h5>
<ul>
<li>We, 19.10.2022, 20:00 (CEST)</li>
</ul>
<p>Here we used an Agilent 6495C LC-MS/MS system and the MRM Proteomics PeptiQuant Plus kit to perform targeted analysis of 270 proteins in leukemia cells following treatment with the enzyme-drug L-asparaginase.</p>
<h5><strong>6. Phenomenex: Method Development Strategies for Peptide Analysis</strong></h5>
<ul>
<li>Th, 20.10.2022, 05:00 (CEST)</li>
</ul>
<p>Topics: The basics of peptides, A deep dive into different phase chemistries, Understand critical parameters.</p>
<h5><strong>7. Waters Corporation: APGC-MS/MS a game changer for routine pesticide analysis: Reaching new heights</strong></h5>
<ul>
<li>Th, 20.10.2022, 11:00 (CEST)</li>
</ul>
<p>The use of GC-MS/MS utilizing APGC has been shown to offer significant improvements in performance over electron ionization (EI) for pesticide residue analysis, in terms of selectivity and specificity.</p>
<h5><strong>8. Technology Networks: A Novel Strategy for Sequencing Larger RNA With Mass Spectrometry</strong></h5>
<ul>
<li>Th, 20.10.2022, 17:00 (CEST)</li>
</ul>
<p>This webinar will present our progress in developing experimental strategies as well as the software tools necessary to support experimental design and data interpretation.</p>
<h5><strong>9. Agilent Technologies: Simultaneous Data Collection - Improving Your UV-Vis Workflows</strong></h5>
<ul>
<li>Fr, 21.10.2022, 19:00 (CEST)</li>
</ul>
<p>When using a UV-VIS Spectrometer for routine measurements, efficiency and throughput are key.</p>


Webinars LabRulezLCMS Week 42/2022

<p><strong>The Clarity MS Extension is a tool that is used for processing data that has been acquired from selected Mass Spectrometry detectors. Spectral data, together with chromatograms, add a third dimension to analytical data analysis.</strong></p>
<ul>
<li><p>👌 <strong>Combination of MS signals</strong> with conventional GC/HPLC detectors</p>
</li>
<li><p>🔝 MS detectors can be used <strong>in combination with 1000+ instruments</strong> supported in Clarity</p>
</li>
<li><p>💡 MS manufacturers can develop <strong>a driver for their own detectors</strong> using a Clarity Software Development Kit (SDK)</p>
</li>
</ul>
<p><strong>The MS Extension</strong> expands the capability of <strong>Clarity</strong> and <strong>Clarity Offline Chromatography software</strong> by providing interactive spectral analysis, peak purity analysis, and compound identification that is based on a spectral library search. The extension is prepared to function with <strong>single quadrupole MS detectors</strong> and <strong>TOF MS detectors</strong>.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_software_Mass_Spectrometry_Extension_1_7fb5562dc3/DataApex-Clarity-software-Mass-Spectrometry-Extension-1.webp" alt="DataApex: Clarity software - Mass Spectrometry Extension"/></p>
<p>All <strong>data is saved in a single file</strong>; a chromatogram at any m/z value or even only a spectrum can simply be recalled for review after an analysis. <strong>MS spectra</strong>, acquired using an MS detector, may be interactively selected <strong>from a chromatogram signal</strong> for visual inspection and comparison. The spectra may also be used for <strong>peak purity determinations</strong> and <strong>component identification</strong> through spectral libraries.</p>
<p>It is possible to import MS data in <strong>AIA</strong> (cdf), <strong>mzXML</strong>, <strong>MzML</strong>, and <strong>mzData</strong> formats.</p>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Data_Apex_Clarity_software_Mass_Spectrometry_Extension_2_6b036064d3/DataApex-Clarity-software-Mass-Spectrometry-Extension-2.webp" alt="DataApex: Clarity software - Mass Spectrometry Extension"/></p>
<p>Control modules for selected detectors (see list of <a href="https://www.dataapex.com/controls"><strong>control modules</strong></a>) are available in Clarity.</p>
<p><strong>Additional control modules for MS detectors can be developed upon request</strong>, please <a href="https://www.dataapex.com/document/contacts"><strong>contact us</strong></a> for availability.</p>
<blockquote>
<h3><strong>Selected supported GC/MS &amp; LC/MS detectors</strong></h3>
</blockquote>
<blockquote>
<h5><strong>LC-MS Detectors</strong></h5>
<ul>
<li><strong>Advion:</strong> Expression</li>
<li><strong>Gilson:</strong> Verity 1920</li>
<li><strong>Konik:</strong> Q4 LCMS</li>
<li><strong>Microsaic:</strong> 4000 MiD</li>
<li><strong>Microsaic:</strong> 4500 MiD</li>
<li><strong>Young In Chromass:</strong> YL9900</li>
</ul>
</blockquote>
<blockquote>
<h5><strong>GC-MS Detectors</strong></h5>
<ul>
<li><strong>Young In Chromass:</strong> ChroZen GC/MS</li>
<li><strong>Young In Chromass:</strong> YL6900</li>
</ul>
</blockquote>
<blockquote>
<h5><strong>GC-MS TOF Detectors</strong></h5>
<ul>
<li><strong>Markes International:</strong> BenchTOF</li>
</ul>
</blockquote>


Clarity software - Mass Spectrometry Extension

ESAS-CSSC 2022 (Day 3)

Article

Product

Events

Webinars

Application

Academy

Video

Poster

Scientists

Plastics

Environment

Food

Water

Health

Presentation

Scientific article

Interview

Toxicology

Popularization

Science and research

Software

Covid-19

Career

Laboratories

Various

PFAS 

Zaměření

Type of News

Focus

Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

DataApex

The Faculty of Pharmacy, with its seat in the town of Hradec Králové, was established by a government decree in 1969. This Faculty of Pharmacy has continued in the old and long-time tradition of the education of pharmacy at Charles University.

Faculty of Pharmacy, Charles University

is the international joint scientific meeting to share recent developments, exchange ideas, explore new directions and initiate a possible collaboration in the analytical spectrometry area.

ESAS

The relatively new tradition of the Czech mass spectrometry conferences was established only in 2011, the very first event took place in Hradec Králové. Since then, the conference was organized every year except for 2020, the year of the pandemic.

Czech Mass Spectrometry Conferences

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

ESAS-CSSC 2022 (Day 3)

Clarity software - Mass Spectrometry Extension

Webinars LabRulezLCMS Week 42/2022

6th STARSS Conference on Separation Science (Program)

Conference CEEPC/IPM/CMSC 2022 (Day 3)

Webinars LabRulezLCMS Week 41/2022

Shimadzu Nexera LC-40 HPLC system supported in Clarity

Conference CEEPC/IPM/CMSC 2022 (Day 2)

Webinars LabRulezLCMS Week 40/2022

Conference CEEPC/IPM/CMSC 2022 (Day 1)

Upcoming events

Bruker at SOFT-TIAFT 2026

Thermo Fisher Scientific at SOFT/TIAFT 2026

Thermo Fisher Scientific Environmental & Food Safety Workshop Series 2026

Webinars

Expanding the Evidence: New Data for More Reliable PFAS Analysis

How is capillary electrophoresis being used to analyze DNA and RNA

A novel monodisperse supermacroporous reversed-phase platform for comprehensive nucleic acid analysis across a broad size range