<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezGCMS Library in the week of 29th September 2026? Check out new documents from the field of the gas phase, especially GC and GC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT GCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by <strong>Agilent Technologies, LECO </strong>and<strong> Shimadzu!</strong></p><h3><strong>1. Agilent Technologies: Sensitive and Reproducible Determination of Organochlorine Pesticides in Water</strong></h3><p><strong>Large volume injection and dual-column confirmation with the Agilent GC/ECD system</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_organochlorine_pesticides_water_gc_ecd_system_5994_9441en_agilent_61787a0901.pdf"><strong>Full PDF for download</strong></a></li></ul><section><div><div><div><div><div><div><p>Organochlorine pesticides (OCPs) remain important environmental contaminants due to their persistence, bioaccumulation potential, and toxicity. Regulatory laboratories often require sensitive and robust analytical methods capable of detecting these compounds at trace levels in environmental samples. USEPA Method 8081B is commonly used for the analysis of organochlorine pesticides using GC/ECD due to the strong electron-capturing properties of halogenated compounds.<sup>1</sup>&nbsp;</p><p>Large volume injection (LVI) techniques can significantly improve detection sensitivity by introducing more analyte mass into the GC system. However, successful LVI implementation requires careful optimization to minimize solvent flooding, peak distortion, and poor chromatographic performance. The Agilent Multimode Inlet (MMI) provides a flexible solvent vent mode that simplifies LVI method development while maintaining analytical robustness.&nbsp;</p><p>In this work, an Agilent GC/ECD system equipped with MMI was evaluated for the quantitative analysis of 31&nbsp;organochlorine pesticides using two analytical columns <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/library?mainauthorItems=10&amp;search=DB-5ms&amp;sources=GCMS_EN"><strong>DB-5ms</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/library?mainauthorItems=10&amp;sources=GCMS_EN&amp;search=DB-1701"><strong>DB-1701</strong></a>. The use of two columns follows the recommendations of USEPA Method 8081, which requires compound identification based on a primary column to be confirmed on a second column with a different stationary phase to improve analyte identification confidence. Calibration performance, unknown sample quantification, IDL determination, and multiday stability performance were&nbsp;assessed.</p><h4><strong>Conclusion</strong></h4><p>The Agilent GC/ECD system equipped with a Multimode Inlet successfully demonstrated robust quantitative analysis of 31 organochlorine pesticides using large volume injection technology. The solvent vent LVI approach enabled high sensitivity while maintaining good chromatographic performance and excellent reproducibility.&nbsp;</p><h5><strong>Key performance highlights included:&nbsp;</strong></h5><ul><li>Successful dual-column setup meeting EPA 8081 confirmatory identification requirements</li><li>Excellent calibration linearity with R² values greater than&nbsp;0.992&nbsp;</li><li>IDLs of 2.39 ppb or lower for all analytes&nbsp;</li><li>Excellent retention time repeatability with %RSD ≤ 0.07%&nbsp;</li><li>Stable multiday peak area reproducibility with %RSD ≤&nbsp;7.83%&nbsp;</li></ul><p>The combination of Agilent GC/ECD instrumentation, MMI solvent vent technology, and optimized large volume injection workflow provides an effective solution for routine environmental monitoring of organochlorine pesticides.</p><h3><strong>2. LECO: Determination of PFAS in Environmental Waters Using DHS-GCxGC-TOFMS</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/PEGBT_4_D_PFAS_ENVIRONMENTAL_WATER_203_821_721_815a93c9bc.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Per- and polyfluoroalkyl substances (PFAS) represent a vast group of synthetic organic compounds, with over 15,000 known variants. Their structure, characterized by strong carbon-fluorine bonds and amphipathic properties, makes them highly resistant to chemical 1 and biological degradation. These unique features led to extensive industrial and domestic applications, including coatings, 1 textiles, firefighting foams, packaging, and cosmetics. However, their persistence and mobility in the environment, along with their bioaccumulative nature, have raised serious health and ecological concerns. PFAS have been detected in human blood, breast milk, and umbilical cords, and studies since the 2000s have linked them to cancer, immune dysfunction, and endocrine 2 disruption.&nbsp;</p><p>Their recognition as persistent organic pollutants (POPs) under the Stockholm Convention in 2001 has prompted global efforts 3 to limit their release. Yet treating PFAS-contaminated water remains challenging, particularly for short-chain congeners that are not included in conventional removal methods. Monitoring is further complicated by their low concentrations and the interference of complex sample matrices. In addition, traditional sample preparation techniques like liquid-liquid extraction 4,5 (LLE) and solid-phase extraction (SPE) are laborious, solvent intensive, and prone to contamination. In this context, the use of thermal desorption tubes (TDTs) is well suited for trace-level compounds. They enable effective preconcentration while 6 minimizing solvent use and sample handling, reducing the risk of contamination.&nbsp;</p><p>This research aimed to leverage dynamic headspace extraction (DHS) combined with thermal desorption as an alternative for the extraction of volatile and semi-volatile PFAS in water samples. Advanced analytical techniques such as gas chromatography coupled to time-of-flight mass spectrometry (GC-TOFMS) and comprehensive two-dimensional gas chromatography (GCxGC)-TOFMS were used to enhance resolution, sensitivity, and selectivity.</p><h4><strong>Experimental</strong></h4><p>Both GC-TOFMS and GCxGC-TOFMS analyses were performed on a <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/37"><strong>Pegasus BT 4D GCxGC-TOFMS system</strong></a> (LECO Corporation, MI, USA) equipped with an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/library?mainauthorItems=10&amp;search=8890&amp;sources=GCMS_EN"><strong>Agilent 8890 GC</strong></a> and an <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/library?mainauthorItems=15&amp;search=RTC-PAL&amp;sources=GCMS_EN"><strong>RTC-PAL autosampler</strong></a> capable of automated handling of desorption tubes by employing a Capping/Decapping tool from GL Sciences (Eindhoven, The Netherlands). Sample injections were carried out using an Optic-4 multimode inlet (GL Science, Eindhoven, The Netherlands) with Peltier cooler and a liner exchanger (i.e., LINEX). The GC system was also equipped with a cryogenic trap, used for the secondary trapping/release stage. ® 7 The instrument parameters are summarized in Table 1. Data were acquired and processed using <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/41"><strong>ChromaTOF software</strong></a>.</p><h4><strong>Conclusions&nbsp;</strong></h4><p>The use of dynamic head-space extraction and thermal-desorption GC(xGC)-TOFMS using the LECO Pegasus BT 4D enabled the determination of nine volatile PFAS targets, including fluorotelomer alcohols, acrylates, and alkyl sulfonamides. The method achieved sensitive and precise quantification, with LOQs down to 6.6 ng L⁻¹ and repeatability between 1.4-7.9% RSD. When compared with 1D GC, the enhanced sensitivity of GCxGC allowed reliable detection of PFAS even at much lower concentrations. Moreover, the superior separation power of GCxGC supported effective non-target screening, enabling the identification of additional environmentally relevant compounds in an industrial water case study.&nbsp;</p><p>The combination of DHS-TD with GC-TOFMS, and particularly with GCxGC, provides a versatile platform that ensures robust quantification, high sensitivity, and expanded capabilities for both targeted PFAS analysis and non-targeted screening, supporting its application in environmental monitoring. Given the presence of hundreds of molecules, many of which are still poorly known and characterized, future studies will extend the workflow by including GC(xGC)-HRMS, which represents an important step toward a more comprehensive characterization of this emerging class of (semi-)volatile contaminants and will support the discovery of unknown congeners.</p><h3><strong>3. Shimadzu: Analysis of Methyl Ethane Sulfonate in Nintedanib Esylate API Sample Using Headspace-Trap Technique</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_06_sip_gc_045_en_423dc17c28.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>Shimadzu GCMS-TQ8050 NX with HS-20 NX autosampler works with LabSolutions GCMS 21CFR compliant software which extendsits utilization in compliance environment like pharmaceuticals</li><li>Trap headspace enables concentrating the analytes in cold trap with multi-injection count and helps to achieve higher sensitivity overstatic headspace, especially for trace level quantitation</li></ul><p><strong>Overview: </strong>Alkyl and aryl sulfonic acids have been used in the synthesis of active pharmaceutical ingredients (APIs) in various measures. They are known for their acid catalytic properties, sulfonamide intermediates and as agents to enhance solubility through salt formation. However, as the reactions progress, they form their respective esters, which are well-known potent genotoxic impurities (GTIs), that act as DNA-alkylating agents in biological systems posing mutagenic and carcinogenic risks even at trace levels. Hence, it is necessary to categorize, qualify, and control these impurities to limit the potential carcinogenic risks, as mentioned in ICH M7(R2).&nbsp;</p><p>Nintedanib Esylate API belongs to a family of kinase inhibitors, used primarily to treat idiopathic pulmonary fibrosis (IPF). It acts as a triple angiokinase inhibitor which targets receptors involved in pulmonary fibrosis. The synthesis of the API often involves the reaction of Nintedanib free base with Ethane Sulfonic Acid in a process called as salification. The salt formed in the form of yellow crystals is used further in formulations to enhance stability as well as solubility. The by-product of this reaction, often due to presence of residual short-chain alcohols, is Methyl Ethane Sulfonate (MES).</p><p>As per the ICH M7(R2) guidelines, a TTC (Threshold of Toxicological Concern) based acceptable intake of a mutagenic impurity of 1.5 μg/day which can be used for most pharmaceuticals as a default value, to derive an acceptable limit for control [2,3]. Nintedanib Esylate is supposed to be administered orally. Strength of the Nintedanib Esylate API in most of the medicines is 150 mg per capsule and prescribed dose is twice in a day. [4]</p><p>Irrespective of the limit calculated from maximum daily dose, Shimadzu has worked upon developing a method for quantification of MES at lowest possible detection so that it will be beneficial for the concern pharmaceuticals even if daily dose exceeds 0.3 g/day.</p><h4><strong>Need for a robust analytical method&nbsp;</strong></h4><p>Nintedanib Esylate API shows high solubility in acidic pH of about 3. To inject acidic aqueous media into GC via direct injection technique creates the risk of very high vapor pressure at the injector port. Further, its entry into the column might affect the stationary phase, thereby drastically reducing the shelf-life of the column. As a result, sensitivity of the analytical method becomes questionable. In order to overcome the said issues, it is imperative to develop a robust analytical method pertaining to high sensitivity, repeatability and reproducibility of the results.</p><p>In this regard, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/library?mainauthorItems=1&amp;search=8050"><strong>Shimadzu GCMS-TQ8050 NX triple quadrupole</strong></a><strong> </strong>system with <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/library?mainauthorItems=1&amp;search=HS-20+NX"><strong>HS-20 NX</strong></a> (Trap) as shown in Figure 2, was used to develop a selective and sensitive method for the determination of Methyl Ethane Sulfonate in Nintedanib Esylate API at trace levels.&nbsp;</p><p>Further, development and partial validation were performed at significantly lower concentrations which clearly evidences the capability of instrument and suitability of the developed method.&nbsp;</p><p>To evaluate the method suitability, few validation parameters were performed such as system suitability, LOQ precision, Linearity and accuracy in terms of spike recovery. Refer Table 1 for the concentration levels of method validation parameters.</p><h4><strong>Conclusion&nbsp;</strong></h4><ul><li>Shimadzu GCMS-TQ8050 NX with HS-20 NX (Trap) autosampler enables accurate quantification of Methyl Ethane Sulfonate in Nintedanib Esylate API sample at ppb level.&nbsp;</li><li>Trap mode in the HS-20 NX auto sampler benefits in extracting the analyte multiple times which enhances sensitivity</li></ul></div></div></div></div></div></div></section>

News from LabRulezGCMS Library - Week 40, 2026

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 29th September 2026? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you technical note by <strong>KNAUER,</strong> application note by <strong>Shimadzu </strong>and posters by<strong> Thermo Fisher Scientific / HPLC Symposium </strong>and <strong>Waters Corporation / AOAC!</strong></p><h3><strong>1. KNAUER: Efficient separation of di(2-ethylhexyl) phthalate (DEHP) from oil matrices using GPC</strong></h3><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VTN_00_XX_knauer_applichrom_gpc_f1d7f1a236.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Phthalates are chemical compounds primarily used as plasticizers in polymers to enhance the flexibility and elasticity of materials such as polyvinyl chloride (PVC) [1]. Since plasticizers are not covalently bound to the polymer chains, they have the potential to leach into the contacting media and contribute to contamination [2]. With the widespread and increasing use of plastic materials, the risk of phthalate release and human exposure continues to rise. As a result, and due to their potentially harmful effects on human health, phthalates have attracted growing scientific and regulatory attention in recent years [1]. Oil-rich food products can leach lipophilic phthalates from their packaging [3]. Throughout the production and packaging process, polymeric components such as tubing, containers, and caps may serve as potential sources of contamination. For the analysis of phthalates in such oil matrices, sample cleanup is an essential step. Gel permeation chromatography (GPC), based on sizeexclusion principles, is widely applied as an effective cleanup technique prior to subsequent analytical determination.</p><h4><strong>MATERIALS &amp; METHODS</strong></h4><p>The samples were dissolved in the eluent mixture and filtered through a 0.45 µm PTFE filter prior to analysis. For both olive oil and canola oil, an additional sample spiked with DEHP was prepared and likewise filtered before analysis. Chromatographic analysis was performed using an isocratic method with an eluent composition of ethyl acetate/heptane (50:50 v/v) at a flow rate of 1.0 mL/min. Detection was carried out by UV absorbance at 254 nm. The injection volume was 8 µL using a full-loop injection mode. The column temperature was maintained at 25°C. Separation was achieved on an <strong>AppliChrom® OleoSep column </strong>(10 µm, 300 × 8 mm). The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1630"><strong>AZURA® Semi Preparative GPC System</strong></a> was used.</p><h4><strong>CONCLUSION</strong></h4><p>GPC sample preparation is an effective approach for separating phthalates from high-molecular-weight matrices such as edible oils. In this study, the successful separation of di(2-ethylhexyl) phthalate (DEHP) from three different oil samples was demonstrated. Based on these results, it can be assumed that other phthalates with lower molecular weights (&lt; 390 Da) can also be effectively separated under same conditions. The KNAUER AZURA® GPC semi preparative system (Fig. 4) in combination with the AppliChrom® OleoSep GPC column provides a robust and efficient setup for sample preparation in phthalate analysis, offering rapid separation, high resolution, and minimal solvent consumption due to the short analysis time.</p><h3><strong>2. Shimadzu: Analysis of Chloride and Sulfate Ions in Bioethanol Using the </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+IC"><strong>Nexera IC Ion Chromatograph</strong></a></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01211_en_55cbf3e64f.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>Analysis of chloride and sulfate ions in fuel ethanol can be conducted in accordance with ASTM D7319-221) .</li><li>Accurate quantitative determination, exhibiting excellent linearity and repeatability, can be achieved at concentration levels significantly below the limits specified by the ASTM criteria.</li></ul><p>First-generation bioethanol produced from raw materials such as corn and sugarcane has been utilized to achieve carbon neutrality. However, research and development for second-generation bioethanol produced from non-food, cellulose-based raw materials is also underway to avoid competition with food crops. In Japan, eight companies, including Toyota Motor Corporation, have formed the “Research Association of Biomass Innovation for Next Generation Automobile Fuels” (raBit) to advance research on next-generation bioethanol technologies. Shimadzu participates as a supporting member and promotes research and development through analytical technology cooperation.</p><p>&nbsp;It is known that the presence of chloride and sulfate ions in fuel ethanol can form engine residue, leading to reduced engine performance. ASTM D4806-252) specifies fuel ethanol quality as criterion of chloride ion must not exceed 6.7 mg/kg and that of sulfate ion must not exceed 4 mg/kg. ASTM D7319-22 includes test methods for chloride and sulfate ions in fuel ethanol using ion chromatography.&nbsp;</p><p>In this application, we present an example of the analysis of chloride and sulfate ions in bioethanol using the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/library?mainauthorItems=1&amp;search=Nexera+IC"><strong>Nexera IC</strong></a> in accordance with ASTM D7319-22.</p><h4><strong>Conclusion</strong></h4><p>Chloride and sulfate ions in bioethanol were determined using a Nexera IC system in accordance with ASTM D7319-22. The method offers high sensitivity, linearity, and repeatability, enabling reliable quantification at concentrations well below ASTM-specified limits, thus supporting effective quality control and regulatory compliance of fuel ethanol.</p><h3><strong>3. Thermo Fisher Scientific / HPLC Symposium: LC-MS analysis of intact and subunit-level mAb enabled by a novel monodisperse supermacroporous reversed-phase platform</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/po_004763_ccs_surepac_protein_reverse_phase_mabs_separation_po004763_na_en_56199ec50d.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Monoclonal antibodies (mAbs) are structurally complex biotherapeutics that require multi-level analytical characterization to ensure product quality, safety, and efficacy. LC-MS analysis is routinely applied at the intact protein and subunit levels to assess molecular mass, heterogeneity, and structural integrity. Enzymatic subunit generation, such as IdeS digestion, enables targeted domain-level characterization while reducing analytical complexity compared to full peptide mapping. Achieving high-performance reversed-phase separations across intact and subunit-level workflows remains challenging, as chromatographic columns optimized for intact proteins often compromise performance of smaller subunits. Consequently, multiple columns are required to support comprehensive mAb characterization.&nbsp;</p><p>In this study, we evaluate a reversed-phase separation column based on a novel 2.5 μm monodisperse supermacroporous (SMP) polymeric stationary phase for unified LC-MS analysis of monoclonal antibodies under intact, reduced, and IdeSdigested conditions. The SMP resin’s uniform particle size and highly accessible pore structure enable efficient separation of both small and large analytes, including intact mAbs, reduced subunits (LC and HC), and IdeS-generated fragments such as scFc and F(ab′)₂. Using the SurePac Protein RP MDi column, baseline separation of these species was achieved within a 10- minute gradient, providing a fast, all-in-one LC-MS approach for mAb variant characterization.</p><h4><strong>Materials and methods</strong></h4><h5><strong>Instruments&nbsp;</strong></h5><p><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/744"><strong>Thermo Scientific Vanquish Horizon UHPLC system</strong></a>; <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/401"><strong>Thermo Scientific Orbitrap Exploris 480 mass spectrometer</strong></a> with <strong>Thermo Scientific OptaMax NG ion source</strong>.&nbsp;</p><h5><strong>Data analysis&nbsp;</strong></h5><p>The UHPLC system with mass spectrometer were operated with <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1758"><strong>Thermo Scientific Xcalibur 4.7 software</strong></a>. Full MS spectra of intact mAbs and mAb fragments were analyzed using Thermo Scientific Freestyle 1.8 and BioPharma Finder 5.4 software that utilizes the ReSpect and Xtract algorithms for molecular mass determination.</p><h4><strong>Conclusions&nbsp;</strong></h4><ul><li>A SurePac Protein RP column enabled a rapid (10 min) separation of mAb LC, HC, scFc, and F(ab′)₂ fragments, with major proteoforms identified by mass agreement.&nbsp;</li><li>The SurePac Protein RP column supports comprehensive intact and subunit-level mAb characterization in a single workflow, with strong separation performance across mAb fragments.</li></ul><h3><strong>4. Waters Corporation / AOAC: IMPROVED PURIFICATION OUTCOMES FOR PROBLEMATIC COMPOUNDS USING AN INERT COLUMN WITH POLAR SORBENT</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/720009616_AOAC_f65ea1b771.pdf"><strong>Full PDF for download</strong></a></li></ul><div><div><section><div><div><div><div><div><div><p>Whether the goal is to obtain enough intermediate for the next step in a synthetic scheme, to polish a final product, or to create a suitable standard for compound identification, preparative liquid chromatography remains a primary technique for isolating compounds from complex mixtures. Recovering the target compound at the desired purity and yield in the least amount of time is of utmost importance.&nbsp;</p><p>Polar targets that do not retain, or “sticky” compounds that seemingly disappear on the column are two groups that many consider to be ’problematic’ in purification. “Sticky” compounds, also known as non-specific adsorbers (NSAs), are metal-sensitive targets what can adsorb to positively charged metal surfaces in the LC system flow path and column, making them difficult to detect and collect effectively. Although other methods, such as column pre-conditioning, can be implemented for isolating compounds known to be interactive, this option can be time-consuming and lead to sample loss and excessive solvent waste.&nbsp;</p><p>Although lab scale purifications in which tens to hundreds of milligrams of target compound are isolated from crude mixtures are still performed routinely, the trend toward smaller scale purification is becoming more prevalent. Laboratories are increasingly opting to isolate only a few milligrams of target compound for preliminary studies. Successful target candidates are then isolated in greater amounts to satisfy ensuing experiments. Whether the objective is to isolate a small amount of target compound for exploratory studies, or to purify greater amounts for their use as reference standards, a column which can resolve eluting impurities efficiently is required.&nbsp;</p><p>Echinacea is an herbaceous flowering plant in the daisy family<sup>1,2</sup> and is one of the most important medical herbs widely used to treat the common cold and other infectious diseases.<sup>3</sup> One of the most abundant compounds in echinacea is chicoric acid (Figure 1), a phenylpropanoid and caffeic acid derivative.<sup>4,5</sup> In this scale-up study, chicoric acid, a suspected “sticky” target polar compound was isolated from a complex echinacea tea extract using two inert XSelect HSS T3 MaxPeak Premier OBD Columns with different particle sizes and dimensions (3.5 μm 10 x 100 mm and 5 μm 10 x 150 mm). The MaxPeak Premier OBD preparative HPLC columns are considered ’inert’ because they are enabled with Waters High Performance Surface (HPS) Technology. The chicoric acid isolated using the 3.5 μm column was of equivalent UV purity to the product isolated using the 5 μm column, with a 34% savings in run time. Smaller particle preparative isolations using columns with inert hardware are a practical option for those laboratories where reduced amounts of target are needed for projects conducted on accelerated timelines.</p><h4><strong>METHODS</strong></h4><ul><li><strong>Analytical Columns and flow rate:</strong> ACQUITY Premier HSS T3 and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1952"><strong>ACQUITY UPLC HSS T3 Columns</strong></a>; 1.8 μm, 2.1x50 mm; 0.35 mL/min&nbsp;</li><li><strong>Prep Columns and flow rate:</strong> XSelect HSS T3 OBD Prep Column and XSelect Premier HSS T3 OBD Prep (custom) Columns, 5 μm, 10x150 mm; XSelect Premier HSS T3 OBD Prep Column, 3.5 μm, 10x100 mm; 5.4 mL/min</li></ul><h5><strong>Instrumentation&nbsp;</strong></h5><ul><li><strong>Analytical:</strong> <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/198"><strong>ACQUITY UPLC H-Class System</strong></a>; Quaternary Solvent Manager, Sample Manager FTN-H, Column Manager, TUV Detector, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1543"><strong>ACQUITY QDa Detector&nbsp;</strong></a></li><li><strong>Prep:</strong> Waters AutoPurification System; 2545 Binary Gradient Module, 2767 Sample Manager, System Fluidics Organizer, 2998 Photodiode Array Detector</li></ul><h4><strong>CONCLUSIONS&nbsp;</strong></h4><ul><li>Chicoric acid was analyzed and isolated using analytical and preparative MaxPeak Premier HSS T3 Columns with inert surfaces, which increased the peak area and height for improved detection and collection.&nbsp;</li><li>Chicoric acid was successfully isolated using 3.5 and 5 μm XSelect Premier HSS T3 OBD Prep Columns and fraction pool analysis showed equivalent UV purity, which suggests the viability of incorporating small particle preparative chromatography into the purification workflow.&nbsp;</li><li>3.5 μm preparative columns improve purification process efficiency and promote sustainability by reducing method run time, solvent usage, and fraction lyophilization time.&nbsp;</li><li>Inert MaxPeak Premier column hardware, OBD Technology, and small particle preparative columns are well-suited for the demands of the purification laboratory when results must be generated efficiently to satisfy accelerated production timelines</li></ul></div></div></div></div></div></div></section></div></div>

News from LabRulezLCMS Library - Week 40, 2026

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 29th September 2026? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><p>This week we bring you application notes by <strong>Agilent Technologies, LECO,</strong> <strong>Shimadzu</strong> and <strong>Thermo Fisher Scientific!</strong></p><h3><strong>1. Agilent Technologies: Analysis of Environmental Waters by ICP-QQQ with a Dual-Cell System and Discrete Sampling</strong></h3><p><strong>Fast, accurate measurements by the </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=10&amp;search=9500"><strong>Agilent 9500 ICP-QQQ </strong></a><strong>in Advanced Helium Mode</strong></p><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_enviro_waters_DCS_9500_icp_qqq_5994_9125en_agilent_590880266a.pdf"><strong>Full PDF for download</strong></a><strong>&nbsp;</strong></li></ul><p>Agilent ICP-MS instrumentation has become standard equipment in many environmental laboratories, recognized for its speed, sensitivity, accuracy, and robustness. Its ability to integrate seamlessly with autosamplers, high‑speed discrete sampling systems, and autodilutors has further contributed to its widespread adoption.<sup>1–3</sup> These capabilities are especially important in contract environmental laboratories, where increasing competition and financial pressures have made sample throughput and productivity a top priority. At the same time, laboratories are expected to process more samples in less time, without sacrificing data quality or ease-of-use. To address these demands, the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=10&amp;search=9500"><strong>Agilent 9500 Triple Quadrupole ICP-MS</strong></a> (ICP-QQQ) includes a unique Dual-Cell System (DCS) collision/reaction cell (CRC). For routine testing of large numbers of samples, the DCS can be operated in Advanced Helium Mode (AHM), a revolutionary collision-cell mode that delivers excellent-quality data across a wide range of analytes.</p><p>AHM delivers high‑sensitivity measurements while effectively removing interferences through a dual mechanism of collision‑induced dissociation (CID) and kinetic energy discrimination (KED), eliminating the need for multiple cell conditions.&nbsp;</p><h5><strong>AHM: A revolution in helium-based interference removal&nbsp;</strong></h5><p>AHM represents a major advancement in He-KED collision-cell technology by dynamically optimizing DCS conditions for both low- and high-mass elements in real time. As a result, AHM provides an approximately 20-fold improvement in sensitivity for low-mass elements and approximately a twofold improvement for mid- to high-mass elements. By enabling interference removal across the entire periodic table in a single gas mode, AHM replaces no gas, conventional He, and high energy (HE) He mode. Eliminating cell‑gas switching simplifies the workflow and accelerates sample measurement using the 9500 ICP-QQQ with DCS.&nbsp;</p><p>As shown in Figure 1, the DCS incorporates a two-stage ion guide design (front and rear ion guides) and rapid cell voltage control. These features underpin the improved performance of AHM relative to conventional He-KED mode. Further technical details of the DCS and AHM are described elsewhere.<sup>4</sup></p><h5><strong>AVS MS discrete sampling: Maximizing sample throughput and minimizing maintenance&nbsp;</strong></h5><p>The 9500 ICP-QQQ can also be fitted with the optional Agilent AVS MS discrete sampling accessory, which is ideal for high-throughput applications. The AVS&nbsp;MS minimizes both sample delivery and rinsing times, significantly accelerating the analytical cycle. When combined with AHM, the 9500 with AVS MS reduces both the analysis time per sample and the interface’s exposure to the complex sample matrices common in environmental samples.</p><p>The AVS MS is an automated multi-port switching valve system that directs sample and rinse solutions through different flow paths.<sup>1,2</sup> It introduces sample to the ICP-MS only during data acquisition, while a clean blank solution is nebulized at all other times. This approach not only increases sample throughput but also reduces the amount of sample that reaches the instrument, resulting in lower cleaning requirements and improved long-term stability.&nbsp;</p><p>By combining AVS MS discrete sampling and a single AHM collision cell mode for all elements, laboratories can achieve higher sample throughput and lower maintenance frequency, ultimately reducing operating costs and improving margins.&nbsp;</p><p>In this study, certified reference materials (CRMs) representative of the routine workload in contract environmental laboratories were analyzed by the 9500 ICP-QQQ to assess accuracy, productivity, and long‑term stability.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>The study showed that the Agilent 9500 ICP-QQQ with Dual-Cell System (DCS) operating in Advanced Helium Mode (AHM) and equipped with an integrated AVS MS discrete sampler delivered exceptional performance for routine environmental analyses.&nbsp;</p><p>Over 140 samples, including real-world environmental water samples, were analyzed in under 140&nbsp;minutes, with no need for matrix matching the calibration standards or recalibration. The 9500 ICP-QQQ workflow delivered a 50% increase in sample throughput compared with a comparable single‑quad ICP‑MS method, substantially improving overall productivity.&nbsp;</p><p>The method’s accuracy was confirmed through multiple measurements of water, soil, and sediment CRMs. Each sample was analyzed in under one minute, highlighting the method’s capacity for high‑throughput analysis while maintaining accuracy, as demonstrated by recoveries within 100 ± 10%. The long-term stability test results confirmed the robust performance of the 9500 ICP-QQQ with UHMI and AVS&nbsp;MS for the analysis of high-TDS samples, with no internal standard failures over more than two hours.&nbsp;</p><p>The results confirm fast analysis, effective interference removal, and robust, stable performance during the analysis of high‑matrix samples over long runs—all critical performance criteria for demanding environmental workflows.&nbsp;</p><p>In an increasingly competitive landscape, where environmental laboratories are under pressure to improve productivity without compromising data quality, the 9500 ICP-QQQ controlled by Agilent OpenLab ICP-MS software operating in a single gas cell mode offers a powerful solution. AHM streamlines analytical workflows, reduces instrument maintenance demands, and delivers robust, reproducible results, enhancing overall operational efficiency.</p><h3><strong>2. LECO: Temperature-Dependent Determination of Total Carbon (TC), Total Organic Carbon (TOC), Residual Oxidizable Carbon (ROC), and Total Inorganic Carbon (TIC) in Soil</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/CM_812_TC_TOC_TIC_ROC_SOIL_203_821_728_4773eacc8f.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Total organic carbon (TOC) analysis is a widely used indicator of soil quality in agricultural and ecological systems. Because TOC reflects the amount of carbon stored in soil organic matter, it serves as a strong measure of soil health, fertility, and its capacity to sequester atmospheric carbon. Organic carbon supports essential soil functions, including microbial activity, nutrient cycling, and soil aggregation, which in turn improves water-holding capacity, reduces erosion, and strengthens the resilience of cropping systems. These qualities are especially important in regenerative agriculture, where restoring ecosystem function is a central goal. TOC measurements are also valuable for evaluating the impact of management practices such as crop rotation, cover cropping, reduced tillage, and the application of compost or manure. By tracking TOC levels, growers can optimize soil amendment strategies to build organic matter effectively without excessive application.&nbsp;</p><p>Residual oxidizable carbon (ROC) is the portion of soil organic carbon that is more stable than actively cycling carbon, yet can still be oxidized through chemical or thermal processes, typically at temperatures between 400–600 °C. Unlike biologically active carbon pools, ROC represents a long-lasting, non-bioavailable carbon reserve made up of materials such as charcoal, biochar, lignin, humus, and graphite. Because these carbon forms decompose slowly, they contribute to durable soil structure, improved nutrient retention, and gradual carbon release. Measuring ROC provides insight into the stability of soil carbon and helps differentiate persistent carbon from rapidly degradable fractions. By comparing ROC to TOC, growers can estimate how much of the soil organic carbon is readily available for biological processes versus how much of it contributes to long-term carbon sequestration.</p><h4><strong>Instrument Model and Configuration&nbsp;</strong></h4><p>The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=24&amp;search=CM812"><strong>LECO CM812</strong></a> is a multiphase carbon and moisture determinator with a variable ramp furnace and a wide carbon detection range that is specifically designed to differentiate various forms of carbon by the temperature at which they combust. This capability facilitates the temperature differentiation determination of the different species of carbon in soil.</p><h4><strong>Typical Data&nbsp;</strong></h4><p>The low carbon calibration was performed utilizing a linear, force through origin calibration using LECO 502-696 (Lot 1005) Synthetic Carbon LCRM (1.01 % C). The high carbon calibration was performed utilizing a linear, full regression calibration using LECO 502-696 (Lot 1005) Synthetic Carbon LCRM (1.01 % C) and LECO 502-905 (Lot 1002) Synthetic Carbon LCRM (5.00 % C). The calibrations were verified using LECO 502-934 (Lot 1002) Synthetic Carbon LCRM (0.53 % C) and a reduced sample mass of LECO 502-905 (Lot 1002) Synthetic Carbon LCRM (5.00 % C). The results are reported on a dry basis.</p><h3><strong>3. Shimadzu: Trace Elements Analysis in Steel by ICP-MS</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/an_01_01207_en_618679c078.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>Even high-matrix metal samples can be analyzed using the Aerosol Dilution System.&nbsp;</li><li>Trace elements in steel can be analyzed simultaneously under a single He collision mode, reducing analysis time.&nbsp;</li><li>Operating costs can be reduced by using a mini torch, which reduces argon consumption.</li></ul><p>In high-performance metallic materials such as steel, material properties are strongly influenced by trace element concentrations, making their proper control extremely important. Conventionally, inductively coupled plasma atomic emission spectrometry (ICP-AES) has been widely used for trace element analysis in metallic materials. In Japan, steel samples have been controlled and analyzed based on JIS G 12011) and JIS G 1258. 2) Recently, however, as metallic materials have become increasingly sophisticated, there has been a growing need to control elements at concentrations below those that can be addressed by conventional methods.</p><p>Inductively coupled plasma mass spectrometry (ICP-MS) is known as a highly sensitive technique for trace element analysis, but in the past it was not always suitable for analyzing high-matrix samples such as metals. In recent years, however, improvements in sample introduction systems, including gas dilution technology, have made it possible to apply ICP-MS to high-matrix samples as well.&nbsp;</p><p>This article describes the trace element analysis of certified reference materials for steel using the Aerosol Dilution System installed as standard in the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=1&amp;search=ICPMS-2050"><strong>ICPMS-2050</strong></a> (Fig. 1). By reducing the amount of sample introduced into the plasma through gas dilution, matrix-induced decreases in signal intensity and signal drift could be effectively suppressed, enabling stable analytical results. The results are presented below.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>In this application news article, trace elements in a certified reference material for steel were analyzed using the<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/icpms/library?mainauthorItems=1&amp;search=ICPMS-2040%2F2050"><strong> ICPMS2040/2050</strong></a>. Agreement with the certified values confirmed the accuracy of the analytical results. In addition, for the elements analyzed in this study, simultaneous analysis under a single condition was possible without changing the cell conditions, thereby reducing the analysis time. By using a mini torch, the ICPMS-2040/2050 reduces argon gas consumption while enabling stable analysis of high-matrix metal samples such as steel.</p><h3><strong>4. Thermo Fisher Scientific: Unbiased Color Analysis using UV-Visible Spectrophotometers</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/unbiased_color_analysis_using_uv_visible_spectrophotometers_an1406_en_b2893661ee.pdf"><strong>Full PDF for download</strong></a></li></ul><p>Across a range of different applications, the color of a material can be an important quality assessment. For certain environments, like textiles and paint manufacturers, the color must match an established criteria to ensure product consistency. In other areas, like water analysis and pharmaceutical manufacturing,¹- ⁴ the color of a product may indicate the presence of undesired substances. While the specific areas of study can vary greatly, the ability to rigorously assess a given sample’s color is shared among these application spaces. Visual methods for determining color have been used in many of these industries; however, technician-to-technician biases inherently influence the analysis. This can lead to discrepancies between testing subjects and testing sites. As a result, it is often preferred to use an unbiased instrument-based method for precisely determining the color of a given product or sample.&nbsp;</p><p>The human eye is able to collect the light reflected off a surface, leading to the perception of color. Since the color range we can see covers the aptly named visible range of the electromagnetic spectrum (400 – 700 nm), UV-Visible spectrophotometers are often used for color analysis. These instruments are used to assess a material’s response to light in the ultraviolet (UV) and visible spectral range. Based on the electronic structure of the material, light within this range is either absorbed, transmitted, or reflected off the material of interest. Given that color perception is based on the collected reflections from a material’s surface, UV-Visible spectroscopy is an ideal technique for this analysis.&nbsp;</p><p>Once the visible spectrum of a given sample, either in liquid or solid form, is collected, there are a number of unique analysis methods that can be used to report the color. As there are many different industries which require this analysis, a multitude of different standardized methods exist which call for different color analysis procedures. Herein, a selection of these standard color analyses is detailed, and information pertaining to the industry standards required for these analyses are discussed. For these methods, special attention is focused on the use of UV-Visible techniques.</p><h4><strong>Summary&nbsp;</strong></h4><p>The color of a finished product can have implications on the quality, both in terms of the appearance and the presence of degraded materials or contaminants. As shown herein, there exist many standardized methods for ascertaining the color of a given substance. While visual methods can be applied, the use of a UV-Visible spectrophotometer is widely accepted and is helpful in providing an unbiased technique for color analysis.</p>

News from LabRulezICPMS Library - Week 40, 2026

Article

Application

Poster

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Merck

Agilent Technologies

ACD/Labs

Petro Industry News

Quantum Analytics

Evosep Biosystems

Waters Corporation

<p><strong>Untargeted metabolomics provides an opportunity to interrogate a sample for systems biology and biomarker discovery for life science researchers.</strong></p>
<p>Discussed here is a comprehensive untargeted analysis workflow for metabolomics.</p>
<p>Plasma samples from mouse models were processed using automated sample prep workflow and analyzed using HILIC chromatography and the 6546 LC/Q-TOF which provided high resolution, extended dynamic range, and isotopic fidelity for the highest confidence in identification. A custom and transferable MS/MS and retention time library for over 400 core metabolites was built for identification. When a true unknown was discovered, SIRIUS/CSI:FingerID software was used for identification based on the MS/MS fragment profile.</p>
<blockquote>
<p><strong>Presenter: Karen Yannell (LC/MS Application Engineer, Agilent Technologies, Inc.)</strong></p>
</blockquote>
<blockquote>
<p>Karen E. Yannell is a LC/MS Application Engineer at Agilent Technologies. She is an expert in small molecule high throughput analysis by triple quadrupole (TQ) and quadrupole time-of-flight (QTOF) mass spectrometry for applied markets. Her recent work has focused on developing routine Q-TOF workflows for both expert and non-expert users alike. Previously, Karen worked on forensic, metabolomics, and biomarker discovery applications with ambient ionization sources. She received her PhD in Analytical Chemistry from Purdue University where studied under the advisement of Prof. R. Graham Cooks. Outside of the laboratory, she enjoys cooking unique dishes and hiking along the beach with her dog.</p>
</blockquote>


Agilent Technologies: Say “I Do” to New ID Tools: A Novel Untargeted Approach to Unveil the Metabolome

Say “I Do” to New ID Tools: A Novel Untargeted Approach to Unveil the Metabolome

Discussed here is a comprehensive untargeted analysis workflow for metabolomics.

A Novel Untargeted Approach to Unveil the Metabolome

<p><strong>Questions that are answered by structure elucidation and &amp; verification are integral parts of chemical R&amp;D workflows from discovery to development and manufacturing. But as scientists constantly strive to do their work faster and better, they must be careful to ensure that efficiency does not come at the cost of confidence in the determined structure.</strong></p>
<p>Join us as we bring together experts from some of the industry’s leading organizations to share how they leverage software to make the most of their NMR, LC/MS, and GC/MS data and boost their structure elucidation and verification workflows.</p>
<h5>Who won’t want to miss this symposium?</h5>
<ul>
<li><p>Synthetic and medicinal chemists responsible for elucidating and verifying the structures of their reaction products and by-products<br/>Natural products researchers</p>
</li>
<li><p>Members of structure elucidation groups</p>
</li>
<li><p>Decision makers at institutions where chemical structures need to be identified quickly and confidently</p>
</li>
</ul>
<h3><strong>Event Agenda</strong></h3>
<h5><strong>15:30-16:10 (CEST) Combined LC/MS and NMR for Automated Verification of Chemical Structures</strong></h5>
<ul>
<li><strong>Celine Nguyen-Van-Dau</strong> (Head of Integrated Drug Discovery (IDD) Structural Analysis team, Sanofi)</li>
</ul>
<h5><strong>16:15-16:50 (CEST) Pharmaceutical Discovery and Development Analytical Workflows</strong></h5>
<ul>
<li><strong>Kevin Robbins</strong> (Associate Principle Scientist, AstraZeneca)</li>
</ul>
<h5><strong>16:55-17:30 (CEST) Conformational Analysis of Peptidomimetic Drug Leads by NMR</strong></h5>
<ul>
<li><strong>Purnima Khandelwal</strong> (Senior Principal Scientist, Small Molecule Drug Discovery, Bristol Myers Squibb)</li>
</ul>
<h5><strong>Hosted By</strong></h5>
<ul>
<li><strong>Sanji Bhal</strong> (Director, Marketing Communications (ACD/Labs))</li>
<li><strong>Sarah Srokosz</strong> (Marketing Communications Specialist (ACD/Labs))</li>
</ul>


ACD/Labs: What's That Peak? Structure Elucidation & Verification Virtual Symposium 

What's That Peak? Structure Elucidation & Verification Virtual Symposium

What’s in my sample? Did I make what I think I made? What’s that peak?

What's That Peak? Structure Elucidation & Verification

<p><strong>Measurements of free hormone levels are routinely performed in clinical applications to help diagnose health concerns. There are two primary methods that have been used for quantification: immunoassays and liquid chromatography tandem mass spectrometry (LC-MS/MS) usually in conjunction with a sample preparatory technique like equilibrium dialysis (ED); with ED considered the “gold-standard.”</strong></p>
<p>A new technology, BioSPME (Bioanalytical Solid Phase MicroExtraction), has been developed to provide a high throughput automatable cleaner sample in a fraction of time compared to ED.</p>
<h5><strong>In this webinar, we will discuss:</strong></h5>
<ul>
<li>What is BioSPME</li>
<li>How does BioSPME work</li>
<li>Several applications of clinically relevant free hormones correlated against validated equilibrium dialysis samples</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> M. James Ross (Senior Scientist)</p>
</blockquote>
<blockquote>
<p>M. James Ross is a senior scientist at MilliporeSigma. In his past 2.5 years with the company, he has focused on sample preparation primarily dealing with biological samples. James received his BA in Chemistry from Hiram College and his Ph.D. from The Ohio State University.</p>
</blockquote>


MERCK: BioSPME: A Novel Sample Preparation of Clinically Relevant Analytes

BioSPME: A Novel Sample Preparation of Clinically Relevant Analytes

A new technology, BioSPME has been developed to provide a high throughput automatable cleaner sample in a fraction of time compared to ED.

BioSPME: A Sample Prep of Clinically Relevant Analytes

<p><strong>We’ll explore polymer characterization, including quick polymer identification for recycling purposes, identifying product defects or failures for quality control, reverse engineering to facilitate product development. We’ll dive into polymer research – an active area of scientific endeavor where performance and a reduced environmental impact are key elements of interest. Learn why vibrational spectroscopy (infrared (IR) and Raman) instruments are useful for the analysis of polymers in an industrial setting, as IR and Raman are highly specific and sensitive tools that are non-destructive and easy to use.</strong></p>
<p>During the session, we’ll offer clear examples that demonstrate the effectiveness of IR and Raman analysis in each of these categories.</p>
<h5><strong>Key webinar take-aways:</strong></h5>
<ul>
<li>The importance of polymer research where performance and a reduced environmental impact are key elements of interest.</li>
<li>Utilizing vibrational spectroscopy (infrared (IR) and Raman) as a useful tool for the analysis of polymers in an industrial setting.</li>
<li>IR and Raman are highly specific and sensitive tools that are non-destructive and easy to use.</li>
<li>Examples will be presented demonstrating the effectiveness of IR and Raman analysis in each of these categories.</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Tom Tague (Applications Manager, Bruker Corporation)</p>
</blockquote>
<blockquote>
<p>Dr. Tom Tague is the Applications Manager for Bruker Optics. He is also a member of the Visiting Advisory Committee of the Metropolitan Museum of Art in New York and the Strategic Advisory Board of Amplified Sciences. Dr. Tague received his Ph.D. from the University of Utah in Physical Chemistry and his B.S. also in Chemistry from the University of Texas at San Antonio. He also conducted his postdoctoral research at the University of Virginia working with Professor Lester Andrews. He is a member of the American Chemical Society, Society for Applied Spectroscopy, American Physical Society, and the Optical Society of America. Dr. Tague is active in developing new methods and instrumentation with the goal of improving the sensitivity and detection limits of spectroscopy related applications. Tom has more than 90 publications and 5 Patents.</p>
</blockquote>


Pixabay/Adrian Malec: Polymer Science A to Z: Getting Answers from FTIR and Raman Analyses

Polymer Science A to Z: Getting Answers from FTIR and Raman Analyses

Let’s tackle the science of polymer analysis using the power of spectroscopy, including identification, quality control and reverse engineering. 

Polymer Science A to Z: FTIR and Raman Analyses

<p><strong>Peptide fractionation is important in proteomics workflows to reduce sample complexity and increase the separation efficiency. This leads to increased dynamic range of the identified peptides compared to conventional single-shot analysis.</strong></p>
<p>The additional number of injections per fractionated sample is a good match with the short overhead time between injections on the Evosep One.</p>
<h4><strong>Optimal analytical strategies for sensitive and quantitative phosphoproteomics using TMT-based multiplexing</strong></h4>
<ul>
<li><strong>Presenter:</strong> Claire Koenig (CPR)</li>
</ul>
<p>Combination of isobaric labeling (TMTs) coupled with offline high-pH fractionation can maximize analysis depth in quantitative phosphoproteomics analysis. To investigate to what extent sample amount affect the sensitivity and the dynamic range of the analysis, we benchmark strategies for quantitative phosphoproteomics with limited peptide input amounts. Based on this research, we present a decision tree to guide phosphoproteomics users to find the best workflow, to reach optimal depth of coverage, based on experimental design and peptide availability.</p>
<p><strong>More to be announced</strong></p>


Fractionation

Peptide fractionation is important in proteomics workflows to reduce sample complexity and increase the separation efficiency. 

<p><strong>In this educational webinar series, we will go back to the basics of  chromatography, mass spectrometry, data analysis and troubleshooting. Understanding the importance of the basics will enable you to be more effective in your laboratory. During each session we will cover a specific aspect of your LC or LC/MS system:</strong></p>
<ul>
<li>The theory of chromatography and requirements for great separations</li>
<li>The basics of your Liquid Chromatograph (LC): Pumps, injectors, and detectors</li>
<li>An Introduction to mass spectrometry (MS) systems</li>
<li>Considerations with System Suitability</li>
<li>Troubleshooting your LC and MS systems</li>
</ul>
<h3><strong>PART 5  |  SERVICE</strong></h3>
<ul>
<li><strong>Troubleshooting your LC and MS Systems in your Laboratory</strong></li>
<li><strong>When to Start Thinking about Instrument Replacement</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Sharon Munisamy, Ph.D. (Principal Systems Specialist, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Sharon Munisamy is a Principal Systems Specialist, based in New England. She specializes in ACQUITY™ UPLC and Time-of-Flight MS systems, providing support to customers as well as mentoring and training new engineers on these products. Prior to joining Waters, Sharon developed LC/MS and ion mobility methods for the study of biomass byproducts and asphaltenes at Baylor University.</p>
</blockquote>
<blockquote>
<p><strong>HOST:</strong> Alexandra (Allie) Ahern (Informatics Product Marketing Manager, Americas Field Marketing, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Allie Ahern has been with Waters for 10 years and is the Informatics Product Marketing Manager within the Americas Field Marketing Organization. Prior to Allie’s current role, she has held other roles at Waters including Service Marketing Product Manager, Implementation Project Manager, and Field Service Engineer.</p>
</blockquote>
<blockquote>
<p><strong>HOST:</strong> Kieron Faherty, Ph.D. (Separations and Mass Spectrometry Field Marketing Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Kieron Faherty is the Separations and Mass Spectrometry Field Marketing Manager for the Americas at Waters Corporation. He received his B.Sc. (Hons) in Chemistry from the University of Reading (Reading, Berkshire, UK) and his doctorate in Chemistry from the University of Massachusetts (Amherst, MA). He has held several field-based positions at Waters, most recently as a Field Applications Specialist focused on biopharmaceutical applications using Time-of-Flight mass spectrometry instrumentation.</p>
</blockquote>


Waters Corporation: Fall Back to Basics - Part 5: Service

Fall Back to Basics - Part 5: Service

PART 5 topics: Troubleshooting your LC and MS Systems in your Laboratory. When to Start Thinking about Instrument Replacement.

<p><strong>Ensuring the safety and efficacy of biotherapeutics from research to development, and then from manufacturing to quality control is essential in order to bring a product to market. Large molecules pose unique challenges for biotherapeutic analyses in that they often requires running a panel of tests to ensure product efficacy and safety. Industry efforts have evolved towards comprehensive analytical workflows that enable researchers to monitor their purity, potency, and critical quality attributes (CQAs) of highly complex and diverse biotherapeutic molecules.</strong></p>
<p>This session will outline the basics of the biopharmaceutical industry and the analytical tools utilized to access the quality and purity of their products. </p>
<h5><strong>Learning Objectives:</strong></h5>
<ul>
<li><strong>Describe the phases of biotherapeutic &#39;pipeline&#39;</strong></li>
<li><strong>Discuss the various modalities that encompass the biopharmaceutical industry and the analytical tools that routinely used to access the quality of these modalities.</strong></li>
<li><strong>Learn the Quality by Design (QbD) approach in the development of biopharmaceutical products and process parameters to access product critical quality attributes (CQA)</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Christopher M. Colangelo, PhD, MBA (Biopharma Business Development Leader, Americas, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Christopher Colangelo, PhD, MBA is the Agilent Biopharma Business Development Leader for the Americas. Prior to joining Agilent, he was the 5th employee and co-inventor of the 454-sequencing platform. In 2003, Chris joined Yale University as a Research Faculty in where he mentored and supervised a research group producing 60+ peer-reviewed publications and directed core research laboratories for four NIH P30 grants, totaling over $21 million worth of research funds. In 2017, Chris joined Agilent to help customers develop, test, and implement end-to-end biopharma workflows.</p>
</blockquote>


Agilent Technologies: Overview of Analytical Tools for Developing the Next Generation of Biopharmaceuticals

Overview of Analytical Tools for Developing the Next Generation of Biopharmaceuticals

This session will outline the basics of the biopharmaceutical industry and the analytical tools utilized to access the quality and purity of their products. 

Developing the Next Generation of Biopharmaceuticals

<p><strong>This webinar will discuss considerations for adaptation and optimization of an AAA pre-column derivatization method from UPLC to HPLC/UHPLC. The following topics will be examined:</strong></p>
<ul>
<li>Shorter run times (i.e., higher throughput and lower solvent consumption) using a new column for AAA vs. traditional HPLC methods</li>
<li>How to accurately separate, identify, &amp; quantify amino acids in cell culture, protein hydrolysates, foods, and feeds </li>
<li>Scaling of AccQ·Tag Ultra UPLC methods for analysis on HPLC systems, including Waters Arc HPLC and ACQUITY Arc Chromatography Systems</li>
</ul>
<h5>Key learning objectives:</h5>
<ul>
<li><strong>How to accurately separate, identify, &amp; quantify amino acids in cell culture, protein hydrolysates, foods, and feeds</strong></li>
<li><strong>Scaling of proven AccQ·Tag Ultra UPLC methods for analysis on HPLC systems, including Waters Arc HPLC and ACQUITY Arc Chromatography Systems</strong></li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Paula Phenix (Principal Product Marketing Manager, Waters Corporation)</p>
</blockquote>
<blockquote>
<p>Paula Phenix is a Principal Product Marketing Manager in the Chemistry and Automation Group at Waters Corporation, where she has been for three years. Prior to the Chemistry and Automation Group, she was a Principal Product Marketing Manager in the LC Instruments Product Management team.</p>
</blockquote>
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<p><strong>Presenter:</strong> Kimberly Martin (Evaluation Scientist II, Waters Corporation)</p>
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<p>Kimberly Martin is part of the System Design and Development Team at Waters Corporation and serves as an Evaluation Scientist II that verifies, validates and tests new instrumentation and products. She has been the company for over 4 years. She has a total of 13 years of analytical chemistry experience including polymer chemistry, food and environmental chemistry, and pharmaceutical chemistry. Kimberly has extensive experience in HPLC, LCMS, GCMS, GC-FID, additional MS technologies and sample preparation.</p>
</blockquote>


Waters Corporation: Updating your HPLC Method for Amino Acid Analysis to Save Time Compared to Traditional HPLC

Updating your HPLC Method for Amino Acid Analysis to Save Time Compared to Traditional HPLC

This webinar will discuss considerations for adaptation and optimization of an AAA pre-column derivatization method from UPLC to HPLC/UHPLC.

Updating your HPLC Method for Amino Acid Analysis

<p><strong>Monitoring the quality of hydraulic and/or lube oils is critical to prevent premature wear of any kind of machinery. In this session we will look at this topic from a new perspective focusing on the combination of titration (potentiometric, thermometric, coulometric Karl Fischer titration) and Vis-Near-Infrared Spectroscopy (Vis-NIRS).</strong></p>
<p>Combining titration (highly sensitive, specific, a widely used primary method) with Vis-NIRS (fast, non-destructive, results for multiple parameters at once) can make your oil condition monitoring and/or lubricant analysis much more efficient, especially, if your laboratory has to analyze large sample series every day.</p>
<p>Attending this session, you will learn and understand:</p>
<ul>
<li>The specific advantages of each method (titration and Vis-NIR-Spectroscopy)</li>
<li>How and when to use these methods to measure critical chemical and physical parameters in lube and hydraulic oils</li>
<li>What thermometric titration is and how it helps to improve robustness of serial acid number testing</li>
<li>How to automate measurements of large sample series to increase efficiency and reproducibility</li>
<li>How Vis-NIRS for lubricant analysis complies with ASTM guidelines and can save costs and time</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Robert Tobler (Application Specialist Titration)</p>
</blockquote>
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<p>Robert Tobler studied chemistry at the Zurich University of Applied Science in Wädenswil, Switzerland. He joined Metrohm in 2015 as an application specialist titration. Since 2018 he has also been involved in stability measurement.</p>
</blockquote>
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<p><strong>Presenter:</strong> Nicolas Rühl, PhD (Product Manager Spectroscopy)</p>
</blockquote>
<blockquote>
<p>Dr. Nicolas Rühl is working in the Competence Center Spectroscopy since 2015. In this time, he has been in charge of developing the near-infrared (NIR) spectroscopy business at Metrohm. Nicolas has a Master in Business Administration and received his PhD in physical chemistry on single-molecule spectroscopy using Vis-NIR and Raman spectroscopy technologies.</p>
</blockquote>
<blockquote>
<p><strong>Moderator:</strong> Tom Lynch CChem FRSC (Independent Analytical Consultant, International Labmate)</p>
</blockquote>
<blockquote>
<p>Tom has 35 years of experience in the petroleum industry, specialising in the delivery of forensic and problem solving /method development capabilities for BP Fuels and Lubricants businesses globally. He was also a member of the BP Science Council representing Analytical Science and led a BP wide Analytical Science network.Tom has published over 30 citable papers, 4 book chapters and has given over 60 presentations at conferences. He is a past Vice President of the Royal Society of Chemistry (RSC) Analytical Division and a past Chairman of the RSC Separation Science Group. In addition, Tom is a recipient of the Silver Jubilee Medal by the Chromatographic Society and a technical achievement award by the Energy Institute.</p>
</blockquote>


Metrohm: Making your quality control of hydraulic and lube oils more efficient

Making your quality control of hydraulic and lube oils more efficient

Combining titration and Vis-Near-Infrared Spectroscopy for higher sample throughput & faster results

Efficient quality control of hydraulic and lube oils

<p><strong>Agilent ICP-MS MassHunter Workstation instrument control and data analysis software manages all aspects of setup, operation and maintenance of Agilent ICP-MS and ICP-QQQ instruments and accessories.</strong></p>
<p>ICP-MS MassHunter is packed with all the features you need for ICP-MS software for the 7700, 7800, 7850, and 7900 ICP-MS software.​ With an all new redesigned interface, ICP-MS MassHunter streamlines the analytical workflow from start to finish. Smart features help you optimize instrument maintenance and let you know more about your samples giving you unprecedented insight into samples and instrument health.</p>
<p>Learn more about how the new ICP-MS MassHunter can transform your ICP-MS workflow in this Part 1 of our ICP-MS Software Bootcamp.</p>
<blockquote>
<p><strong>Presenter:</strong> L. Craig Jones (ICP-MS Application Scientist, Agilent Technologies, Inc.)</p>
</blockquote>
<blockquote>
<p>Craig has been with Agilent for over 15 years as an ICP-MS applications scientist. He has been involved with multiple type of applications for ICP-MS, including environmental, pharmaceutical, nutraceutical, semiconductor, geologic, and clinical analyses, to name a few. Previous to Agilent he worked in an environmental lab performing analysis and supervising both the inorganic and organic sections of the laboratory. In his spare time, Craig enjoys volunteering at the local marine science center, mountain biking, hiking and relaxing at the beach. Craig obtained a bachelor of science degree in chemistry from Fort Lewis College in Durango, CO.</p>
</blockquote>
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<p><strong>Presenter:</strong> Bert Woods (Application Scientist, Agilent Technologies, Inc.)</p>
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<p>Joined the Agilent ICP-MS team in 2004, with previous employment in the semiconductor industry with Dominion Semiconductor (IBM/Toshiba) and Micron. Bert is a 1997 Chemistry graduate of Radford University in Virginia and an avid Washington DC Sports fan.</p>
</blockquote>


Agilent Technologies: Software BootCamp Kick-off - ICP-MS Part 1

Software BootCamp Kick-off - ICP-MS Part 1

Learn more about how the new ICP-MS MassHunter can transform your ICP-MS workflow in this Part 1 of our ICP-MS Software Bootcamp.