Untargeted Metabolomics Identifies N-Lactoyl-Amino Acids as Dose-Responsive Plasma Biomarkers of Metformin Adherence in Type 2 Diabetes

Analytical investigation of metal distribution from e-cigarette aerosols to lung deposition using multi-platform mass spectrometry

<p><strong>In the week of June 22, the following webinars await you in the field of gas / solid phase and especially Raman, FTIR, GC and GC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Mettler-Toledo: Removal of tire wear in stormwater tanks: In Situ Microflotation with TGA TED-GC/MS Analysis</strong></h5><ul><li>Tu, 23.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6269">Registration</a></li></ul><p>Improve tire wear removal in stormwater tanks. Learn how in-situ microflotation separates hydrophobic microplastics and how TGA TED-GC/MS quantifies tire road wear particles (TRWP) to meet water standards.</p><h5><strong>2. Restek Corporation: EMEA live | Mass Spectrometry - Can It Really Be So Easy?</strong></h5><ul><li>Tu, 23.6.2026 14:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6274">Registration</a></li></ul><p>Demystify mass spectrometry. Learn the essential theory, decode common MS terminology, and discover practical tools to solve daily analytical challenges.</p><h5><strong>3. Separation Science: Safeguarding GC-MS Workflows: A Practical Guide to Switching from Helium to Hydrogen Carrier Gas</strong></h5><ul><li>We, 24.6.2026 11:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/webinar/6277">Registration</a></li></ul><p>Switch from helium to hydrogen carrier gas in GC-MS. Learn practical safety steps, method validation, and how to cut analytical cycle times by up to 40% using on-demand hydrogen generators.</p>

Webinars LabRulezGCMS Week 26/2026

<p><strong>In the week of June 22, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Simplifying Complexity in GLP-1 Receptor Agonist Analysis: From Structural Characterization to Impurity Monitoring</strong></h5><ul><li>Tu, 23.6.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6275">Registration</a></li></ul><p>Simplify GLP-1 receptor agonist analysis. Learn how a streamlined LC-MS workflow combines UHPLC and high-resolution MS for peptide characterization and impurity monitoring.</p><h5><strong>2. Tosoh Bioscience: Tosoh Tuesday - Exploring the AAV Toolkit</strong></h5><ul><li>Tu, 23.6.2026 15:45 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6273">Registration</a></li></ul><p>Improve AAV analysis and purification. Learn how to increase recovery and purity using the TOYOPEARL GigaCap Q-650S resin and columns from Tosoh Bioscience.</p><h5><strong>3. Phenomenex: Sustainable Strategies for High Sensitivity Analysis for Clinical Research LC-MS/MS Methods</strong></h5><ul><li>We, 24.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6278">Registration</a></li></ul><p>Optimize clinical LC-MS/MS methods using sustainable ethyl acetate SLE to extract steroids, MMA, and estrogens from human serum with high recovery.</p><h5><strong>4. Agilent Technologies: Preparing for LC Analysis: What to Consider Ahead of Time</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6288">Registration</a></li></ul><p>Avoid chromatography problems before they start. Learn how to prepare sample matrices, select and maintain LC columns, and establish an effective system housekeeping list.</p><h5><strong>5. SelectScience: Next-generation metabolomics: From annotation to discovery at scale</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6281">Registration</a></li></ul><p>Explore how next-generation mass spectrometry, ion mobility, and advanced computational tools accelerate confident small-molecule annotation and discovery in metabolomics.</p><h5><strong>6. Waters Corporation: Clear the Noise: Improving Resolution of GLP-1 Receptor Agonist and Insulin Impurities</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6262">Registration</a></li></ul><p>Learn how to gain greater resolution for your GLP-1 RA and insulin impurity analysis separations.</p><h5><strong>7. Shimadzu Corporation: Meet LCMS-8065XE: Achieving High-Throughput Ultra-Trace Analysis</strong></h5><ul><li>Th, 25.6.2026 08:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6290">Registration</a></li></ul><p>In this webinar, we will analyze real-world performance data demonstrating how LCMS-8065XE enables high sensitivity analysis even in complex matrices.</p><h5><strong>8. Waters Corporation: Creating Better Chromatographic Methods Using a Systematic Approach</strong></h5><ul><li>Th, 25.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6284">Registration</a></li></ul><p>Stop guessing and start separating. Learn a structured, systematic approach to LC method development to improve robustness and accelerate column selection.</p><h5><strong>9. Waters Corporation: Lower the Flow to see even more</strong></h5><ul><li>Th, 25.6.2026 15:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6285">Registration</a></li></ul><p>Boost large molecule sensitivity in bioanalysis. Learn how switching to microflow LC-MS/MS improves limits of quantification, cuts costs, and reduces solvent usage.</p><h5><strong>10. Separation Science: Advancing PFAS Analysis: From Hidden Precursors to Food Safety Workflows</strong></h5><ul><li>Th, 25.6.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6286">Registration</a></li></ul><p>Discover practical approaches for detecting, analyzing, and addressing PFAS contamination in environmental and food-related samples.</p><h5><strong>11. Waters Corporation: Achieving Accurate BOD Results: What Your Questions Revealed</strong></h5><ul><li>Th, 25.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6283">Registration</a></li></ul><p>Get consistent and defensible BOD results by learning how to troubleshoot common testing errors and standardize your laboratory procedures.</p><h5><strong>12. SelectScience: Future-ready biobanking starts with smarter sample storage</strong></h5><ul><li>Fr, 26.6.2026 16:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6289">Registration</a></li></ul><p>Safeguard your specimens in biobanking and clinical research. Learn from Thermo Fisher Scientific experts how to optimize sample storage, traceability, and compliance.</p><h5><strong>13. SelectScience: Enhancing Empower software data with cloud-based applications</strong></h5><ul><li>Fr, 26.6.2026 17:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/6287">Registration</a></li></ul><p>Connect your chromatography data with Waters’ new cloud-based software solutions. Learn how to track system status in real time and enable AI-powered data analytics.</p>

Webinars LabRulezLCMS Week 26/2026

<p><strong>In the week from June 22, the following webinars await you in the field of atomic and molecular spectroscopy and spectrometry</strong></p><blockquote><h4><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></h4><p><strong>👉 Search and filter in the largest global database</strong> with over <strong>5 500 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Mettler-Toledo: Removal of tire wear in stormwater tanks: In Situ Microflotation with TGA TED-GC/MS Analysis</strong></h5><ul><li>Tu, 23.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6269">Registration</a></li></ul><p>Improve tire wear removal in stormwater tanks. Learn how in-situ microflotation separates hydrophobic microplastics and how TGA TED-GC/MS quantifies tire road wear particles (TRWP) to meet water standards.</p><h5><strong>2. Anton Paar: Heated Stories: When Compliance Heats Up - Take Back Control With Julia Suite Premium</strong></h5><ul><li>Tu, 23.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6270">Registration</a></li></ul><p>Turn pharma regulatory pressure into an efficient workflow. Learn how Julia Suite Premium ensures 21 CFR Part 11 compliance with electronic signatures and permanent audit readiness.</p><h5><strong>3. Agilent Technologies: Enhancing Lithium-Ion Battery Production: Automated and Sustainable Elemental Analysis Using an ICP-OES</strong></h5><ul><li>Tu, 23.6.2026 20:30 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6068">Registration</a></li></ul><p>Join us to see how a smart ICP-OES and an auto-dilutor can be used together to increase productivity, sustainability, and accuracy of your analysis of these challenging sample matrices.</p><h5><strong>4. AZoM: Advancing Process Mineralogy with Automated Multimodal Microscopy</strong></h5><ul><li>We, 24.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6276">Registration</a></li></ul><p>Advance process mineralogy with ZEISS microscopy. Learn how AI and automation optimize automated mineralogy using both light microscopes and SEM to improve mine site efficiency and reduce costs.</p><h5><strong>5. Bruker: Electrical Contacts and Engineered Plastics: Advanced Tribology Testing with UMT TriboLab</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6280">Registration</a></li></ul><p>Discover how Bruker’s UMT TriboLab enables precise electromechanical characterization of dielectric contacts and advanced tribological testing of engineered plastics.</p><h5><strong>6. C&amp;EN: NMR-Augmented Workflows: From Automated Sample Creation to Connected Data Ecosystems Episode 2</strong></h5><ul><li>We, 24.6.2026 17:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6279">Registration</a></li></ul><p>Discover how combining automated sample preparation with digital NMR analytics and enterprise IT systems creates streamlined, scalable, and future-ready laboratory workflows.</p><h5><strong>7. Anton Paar: Heated Stories: What DSC Reveals About Food Products</strong></h5><ul><li>Th, 25.6.2026 10:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6282">Registration</a></li></ul><p>Discover how differential scanning calorimetry (DSC) analyzes the thermal behavior of fats, proteins, and starches to optimize quality control and food product development.</p><h5><strong>8. Mettler-Toledo: Thermal Analysis of Nanomaterials</strong></h5><ul><li>Th, 25.6.2026 16:00 CET</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/5775">Registration</a></li></ul><p>Join our live webinar to explore how thermal analysis techniques can reveal the unique properties of nanomaterials.</p><h5><strong>9. Agilent Technologies: Simplify ICP-MS Maintenance while Improving Sample Throughput in your Laboratory</strong></h5><ul><li>Th, 25.6.2026 19:00 CEST</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/webinar/6069">Registration</a></li></ul><p>This webinar will discuss innovations that reduce routine maintenance and simplifying sample analysis challenges, while leveraging enhancements to increase ICP-MS throughput and free up your time.</p>

Webinars LabRulezICPMS Week 26/2026

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezGCMS Library in the week of 22nd June 2026? Check out new documents from the field of the gas phase, especially GC and GC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT GCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you brochure by <strong>Agilent Technologies, </strong>application note<strong> </strong>by<strong> Shimadzu </strong>and posters by <strong>MDCW / William &amp; Mary </strong>and <strong>Waters Corporation / ASMS!</strong></p><h3><strong>1. Agilent Technologies: Operational efficiency for your GC and GC/MS labs with the </strong><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=8890B"><strong>Agilent 8890B gas chromatography system</strong></a></h3><ul><li>Brochure</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/br_8890b_gc_5994_9205en_agilent_fb5db29527.pdf"><strong>Full PDF for download</strong></a></li></ul><section><div><div><div><div><div><div><h4><strong>Analyze more samples every day with faster cycle times&nbsp;</strong></h4><p>The <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=8890B"><strong>8890B GC </strong></a>provides faster maximum oven temperature ramps and cooldown rates for conventional dual-channel GC. If you want even faster cycle times, an optional rapid cooling fan is available.&nbsp;</p><h4><strong>Achieve excellent repeatability across multiple injections&nbsp;</strong></h4><p>Consistent retention time and area count performance are the cornerstone of what you can expect from the 8890B GC. The system also meets all specifications required for regulatory methods such as USP Method &lt;467&gt; (residual solvents).</p><h4><strong>Shine a new light on your connections and components&nbsp;</strong></h4><p>Make maintenance easier and faster with a built-in oven light that illuminates the internal space on the 8890B GC. That means you can perform tasks like troubleshooting and column installation in less time—and with fewer errors.</p><h4><strong>Boost GC performance with capillary flow technology&nbsp;</strong></h4><ul><li><strong>Backflush</strong> significantly reduces timing between injections, increasing productivity.&nbsp;</li><li><strong>Purged Ultimate unions</strong> provide leak-free connections, reducing downtime.&nbsp;</li><li><strong>Flow splitters</strong> enable data collection from up to three detectors.&nbsp;</li><li><strong>New CFT calculators</strong> let you download calculated setpoints to your method—avoiding time-consuming, error-prone manual input.&nbsp;</li><li><strong>Multidimensional chromatography:&nbsp;</strong><ul><li><strong>Deans Switch</strong> facilitates heart cutting, enabling resolution of trace compound in complex matrices.&nbsp;</li><li><strong>GC x GC flow modulation</strong> enables multidimensional chromatography without the need for cryogen</li></ul></li></ul><h4><strong>Helium conservation module&nbsp;</strong></h4><p>Extend the life of your helium tanks by up to 30 times. The Agilent helium conservation module allows you to use helium for your GC runs and switch to a different gas (typically nitrogen) when your GC is idle. That means you can achieve better control of laboratory operating expenses and avoid workflow disruptions.</p><h4><strong>Alternative carrier gas support&nbsp;</strong></h4><p>Alternative carrier gases like hydrogen and nitrogen are popular low-cost options.&nbsp;</p><p>The <strong>Agilent Hydrogen Sensor Module Series 2 </strong>option adds built-in peace of mind when operating with H2 gas.&nbsp;</p><p>The <strong>Agilent HydroInert source </strong>minimizes sensitivity loss and spectral anomalies associated with H2. It also:&nbsp;</p><ul><li>Maintains spectral fidelity, even for compounds highly susceptible to hydrogenation.&nbsp;</li><li>Improves peak shape for high-boilers, especially polycyclic aromatic hydrocarbons (PAHs)</li></ul><h3><strong>2. MDCW / William &amp; Mary: Decomposition Analysis Using Differing Data Processing Methods to Identify Volatile Organic Compounds</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/P_25_Weina_2ae94f0998.pdf"><strong>Full PDF for download</strong></a></li></ul><section><div><div><div><div><div><div><p>When a body decomposes it emits volatile organic compounds (VOCs). VOCs from decomposing remains have been studied before,1 however there is a gap that remains in the knowledge on VOCs that evolve from submerged decomposing remains. 2 In this study, submerged animal tissue was tested to see how the VOCs can be utilized in forensic settings.&nbsp;</p><p>This study used data obtained last summer using comprehensive two-dimensional gas chromatography – time-of-flight mass spectrometry (GC×GC-TOFMS). Pigs (Sus scrofa domesticus) are often used as analogs in decomposition studies due to similarities with humans. 3 In this study, pork belly samples were submerged in mason jars filled with water and stored at different temperatures. The GC×GC-TOFMS instrument was used to collect odor from above water samples for a period of twelve days.</p><p>GC×GC is often used to analyze complex odor profiles. When analyzing samples with GC×GC, there is a lack of standardization to process the data acquired from time trials. There is a need to discover effective ways to track VOCs for chemical identification and longitudinal analysis.</p><p>The goal of this research was to compare different software approaches to analyze GC×GC longitudinal data to achieve accurate analyte identification, representation of data over time, and effective class characterization.</p><h4><strong>Methods</strong></h4><p>For the first four days, 5 mL of water was taken and run on the <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/37"><strong>GC×GC-TOFMS</strong></a>. Thereafter, the samples were sampled and run every three days until day twelve. Data were analyzed initially using <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/41"><strong>ChromaTOF</strong></a> (LECO Corporation). The samples were then analyzed using <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/348"><strong>ChromaTOF Tile </strong></a>and <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/1873"><strong>ChromaTOF Sync 2D</strong></a> to compare how they can be used in a forensic setting.</p><p>Peak tables were curated in Tile and Sync 2D. Then, ChromaTOF was used as a guide to confirm which peaks were identified correctly. If the compound was correctly identified, the peak would be accepted or kept on the table. If not correctly identified, the peak would either be rejected or deleted. Once the tables were complete from the different software approaches, they were exported to Excel for further comparison.</p><p>In excel, the relevant hits that were identified as decomposition VOCs were compared across the software approaches. The compounds that were most commonly found were compared to existing literature on decomposition VOCs. From there, two tables were built; where one table was compounds confirmed with literature and the other table was compounds not confirmed with literature.</p><p>Comparison between software approaches included looking at different criteria to see which method was the most effective and efficient. The criteria included how long the software approach took, the number of compounds found in each software approach, the accuracy of peak area in the software approach compared to ChromaTOF data, and principal component analysis (PCA) comparison to see which software approach was best used for forensic practice or research.</p><h4><strong>Conclusions</strong></h4><p>Sync 2D showed the capability of showing trends in decomposition compounds</p><ul><li>This software approach can be used in research environment where longitudinal data is needed to determine the trends of decomposition data&nbsp;</li><li>Best purpose: research applications requiring longitudinal tracking&nbsp;</li></ul><p>Coefficient of Variance’s and Fold Change’s PCA plots showed their ability for clear class differentiation&nbsp;</p><ul><li>The two software approaches can be used to distinguish water containing decomposition in a crime scene&nbsp;</li><li>Best purpose: comparison at a single point and time [detailed analysis]&nbsp;</li></ul><p>Fisher Ratio showed a proficiency in identifying top differentiating compounds and showed aptitude for class differentiation&nbsp;</p><ul><li>The software approach would be helpful for cursory analysis for crime scene evidence&nbsp;</li><li>Best purpose: quick comparison of biggest difference for swift identification</li></ul><h3><strong>3. Shimadzu: Determination of Ester and Linolenic Acid Methyl Ester Contents in Biodiesel According to EN 14103:2020</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_05_sca_180_054_en_93d1029b72.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=2060"><strong>NexisTM GC-2060</strong></a> provides reliable analysis of biodiesel samples regarding their ester and linolenic acid methyl ester contents using hydrogen as a cost-effective carrier gas&nbsp;</li><li>It delivers excellent repeatability and provides appropriate safety measures for the use of hydrogen as carrier gas&nbsp;</li><li>The optional multi-mode injection unit (MMI) offers performance equivalent to a conventional split-splitlessinjection unit (SPL), significantly reducing maintenance downtime</li></ul><p>Biofuels play an important role in enabling countriesto meet their greenhouse gas reduction targets. They can be produced from a range of raw materials, including plant oils, animal fats, and used cooking oils. Biodiesel is mainly being produced from rapeseed, soybean, and palm oils. Due to this diversity of feedstocks, continuous quality control is necessary as the quality of the raw material has an enormousimpact on the quality of the biodiesel.&nbsp;</p><p>The EN 14103:2020 describes the determination of total ester content and linolenic acid methyl ester content in biodiesel by means of gas chromatography with flame ionization detection (GC-FID). The total ester content covers a range of fatty acid methyl esters (FAME) from C6 to C24, and linolenic acid methyl ester is investigated separately as interfering reactive compound that might influence long-term stability of the biodiesel. According to the standard, the total ester content must be greater than 90 % (m/m) and the linolenic acid methyl ester content shall be between 1 and 15 % (m/m)1.</p><p>This study shows the analysis of biodiesel originating from rapeseed,soybean, and palm oil using the Nexis GC-2060.</p><h4><strong>Multi-Mode Injection Unit as Alternative&nbsp;</strong></h4><p>Shimadzu’s multi-mode injection unit (MMI) offers reliable splitsplitless mode functionality over a broad range of carbon numbers. It allows analysis of biodiesel samples with high repeatability and provides chromatographic results comparable to those measured with SPL as dedicated split-splitless injection unit. The use of MMI significantly shortens maintenance downtime from tens of minutes for a classical split-splitless injection unit to just a few minutes. Figure 3 gives a direct comparison of an exemplary rapeseed-based biodiesel sample measured with SPL (top) and MMI (bottom).</p><h4><strong>Conclusion</strong></h4><p><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=2060"><strong>Nexis GC-2060</strong></a> provides reliable analysis of biodiesel samples regarding their ester and linolenic acid methyl ester content according to EN 14103:2020, covering the various ester types commonly in use. It allows repeatable analysis using hydrogen as a cost-effective carrier gas. The optional multi-mode injection unit (MMI) can be selected as alternative to a classical splitsplitlessinjection unit (SPL).</p><h3><strong>4. Waters Corporation / ASMS: Putting the (Atmospheric) Pressure on Forever Chemicals: A Targeted Analysis of GC Amenable PFAS using APGC–MS/MS</strong></h3><ul><li>Poster</li><li><a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/720009413_dfa4082b45.pdf"><strong>Full PDF for download</strong></a></li></ul><p>This poster presents a targeted analytical method for the determination of gas chromatography (GC)-amenable PFAS compounds using atmospheric pressure gas chromatography coupled with tandem mass spectrometry (APGC-MS/MS). The study addresses a key limitation of conventional LC-MS/MS methods, which are highly effective for many PFAS but less suitable for more volatile species such as fluorotelomer alcohols (FTOHs), fluorotelomer acrylates (FTAcr), and fluorotelomer acetates (FTOAc). By incorporating GC-MS/MS analysis, laboratories can obtain a more complete characterization of PFAS contamination across environmental, biological, and food-contact materials.</p><p>The analytical workflow utilized an <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=10&amp;search=8890"><strong>Agilent 8890 gas chromatograph</strong></a> equipped with an autosampler and coupled to a <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/554"><strong>Waters Xevo TQ Absolute tandem mass spectrometer</strong></a> operated with APGC ionization in positive-ion mode. Separation was performed on an Rtx-200 capillary column, while data acquisition and quantification were managed using <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1544"><strong>waters_connect software</strong></a>. Unlike traditional electron ionization (EI), APGC generated intact protonated molecular ions, significantly improving sensitivity and selectivity for GC-amenable PFAS. A method covering 35 PFAS compounds was developed, achieving lower limits of quantification (LLOQs) of 0.025–0.1 ng/mL for most analytes.</p><p>Several sample types were evaluated, including wastewater, surface water, soil, fish tissue, shrimp, and food-packaging materials. Environmental and biological samples were prepared according to EPA Method 1633A and had previously been analyzed by LC-MS/MS, allowing the GC-MS/MS method to serve as a complementary technique. Food-packaging samples were extracted with methanol containing ammonium hydroxide and analyzed for targeted PFAS. While no target PFAS were detected in the tested packaging materials, multiple GC-amenable PFAS were found in environmental samples. Fluorotelomer alcohols were the most frequently detected compounds, with wastewater and ski-wax-impacted soils showing the highest concentrations.</p><p>The results demonstrate that APGC-MS/MS provides a sensitive and robust solution for the analysis of volatile PFAS classes that may be missed by LC-MS/MS alone. The method delivered accurate quantification, effective chromatographic separation, and reliable recovery in spiked samples. The authors conclude that combining APGC-MS/MS with established LC-MS/MS workflows enables a more comprehensive assessment of PFAS contamination, particularly for emerging fluorotelomer compounds and other GC-amenable PFAS that are increasingly relevant in environmental monitoring and food-packaging investigations.</p></div></div></div></div></div></div></section></div></div></div></div></div></div></section>

News from LabRulezGCMS Library - Week 26, 2026

News from LabRulezLCMS Library - Week 26, 2026

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezICPMS Library in the week of 22nd June 2026? Check out new documents from the field of spectroscopy/spectrometry and related techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT SPECTROSCOPY/SPECTROMETRY RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><strong>LabRulezLCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> libraries.</p></blockquote><p>This week we bring you technical note by <strong>Agilent Technologies</strong>, application note by <strong>Shimadzu </strong>and other document by <strong>Thermo Fisher Scientific!</strong></p><h3><strong>1. Agilent Technologies: Dual-Cell System (DCS) and Advanced Helium Mode (AHM)</strong></h3><p><strong>Technological developments for higher sensitivity and better interference removal than traditional He-KED</strong></p><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/te_batch_conversion_tool_9500_icp_ms_5994_9214en_agilent_0a1bba76e2.pdf"><strong>Full PDF for download</strong></a></li></ul><h4><strong>Evolution of Agilent’s Collision Reaction Cell (CRC) technology&nbsp;</strong></h4><ul><li><strong>2001: </strong>Launched the first ICP-MS with CRC—the Agilent 7500c with Octopole Reaction System (ORS)&nbsp;</li><li><strong>2011:</strong> Introduced High Energy Helium Mode (HEHe) using Collision Induced Dissociation (CID)&nbsp;</li><li><strong>2012: </strong>Released the world’s first Triple Quadrupole ICP-MS—the Agilent 8800 ICP-QQQ&nbsp;</li><li><strong>2016:</strong> Launched the second generation Triple Quadrupole ICP-MS with Axial Acceleration—the Agilent 8900 ICP-QQQ&nbsp;</li><li><strong>2026: </strong>Introduced the innovative Dual-Cell System (DCS) and Advanced Helium Mode (AHM) for the Agilent 9500 ICP-QQQ</li></ul><p>Collision/Reaction Cell (CRC) technology is now a standard feature in modern ICP-MS systems, providing a reliable means of eliminating spectral interferences that compromise data quality. ICP-MS systems with integrated CRCs range from single quadrupole (SQ) ICP-MS used in environmental and food analysis, to triple quadrupole ICP-MS (ICP-QQQ) for advanced research applications. For routine applications, Helium Kinetic Energy Discrimination (He KED) mode, commonly referred to as helium mode (He mode), is the most widely used CRC operational mode. The widespread use of He mode is due to its universal effectiveness in reducing a broad range of polyatomic interferences without the need for element-specific optimization.&nbsp;</p><p>Unlike CRCs that rely on reactive cell gases and require complex tuning (often referred to as reaction cells), He mode effectively mitigates many commonly occurring polyatomic ion interferences without the need for intricate adjustments. In contrast, reaction cells often require careful selection of gas type (such as ammonia or oxygen) and tuning parameters based on the specific interfering ions on analytes of interest.&nbsp;</p><p>Building on the simplicity of He mode, Agilent has developed Advanced Helium Mode (AHM) for the <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library?mainauthorItems=10&amp;search=9500"><strong>Agilent 9500 Triple Quadrupole ICP-MS (ICP-QQQ)</strong></a>. AHM represents a breakthrough in interference removal technology, enabled by the newly designed Agilent Dual-Cell System (DCS) and high-speed cell voltage control. These innovations deliver high sensitivity across the mass range and superior interference removal performance for routine applications. Compared to conventional He mode, AHM demonstrates the following improvements:</p><ul><li>Around a 20-fold sensitivity improvement for low-mass elements, such as beryllium (Be) and boron (B)&nbsp;</li><li>Around a 2-fold improvement for mid-to-high mass elements&nbsp;</li><li>A reduction of more than 33% in data acquisition time while maintaining detection limits (DLs), accelerating analytical measurement times&nbsp;</li></ul><p>AHM not only replaces traditional He KED but also consolidates multiple tuning modes including no gas, He, and HEHe into a single, streamlined mode. Integrated into the 9500 ICP-QQQ, AHM is a next-generation standard He mode that is faster, simpler, and delivers higher performance than its predecessor. This technical note describes the performance enhancement mechanisms of the DCS and AHM. It also includes some comparative results with conventional He KED mode to highlight the analytical improvements of AHM.</p><h4><strong>Overview and advantages of AHM&nbsp;</strong></h4><p>Typically, multi-element ICP-MS methods apply no gas mode to the determination of low-mass elements like Be and B due to low sensitivity in traditional He KED mode. Consequently, many routine ICP-MS applications have required multiple tune setups, combining no gas, He, and HEHe. Each mode switch introduces a delay due to cell pressure stabilization requirements.&nbsp;</p><p>In practice, low-mass elements (m/z &lt; 23) are generally unaffected by polyatomic ion interferences, so KED is often unnecessary. Therefore, in AHM, different voltages are applied to the DCS (effectively disabling KED) for low-mass elements, while keeping standard settings for elements with m/z ≥ 23.&nbsp;</p><p>The DCS voltages are dynamically switched in sync with Q2 using a high-speed voltage control circuit, providing over 20× sensitivity improvement for low-mass elements compared to conventional He mode. AHM eliminates the need to run in no gas mode, greatly simplifying the method. As a default approach, AHM alone is suitable for most applications.<sup>1</sup></p><h3><strong>2. Shimadzu: Damping Force Characteristic Test of Suspension Struts [JASO C611]</strong></h3><ul><li>Application note</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/an_01_01017_en_2fbbb9c247.pdf"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>Damping force characteristic tests on suspension struts can be performed based on the JASO C611 standard.&nbsp;</li><li>Combination test enables automatic speed change and measurement.&nbsp;</li><li>High responsiveness of the EMT series enables testing at high excitation speed.</li></ul><p>Various components play an important role in achieving a comfortable ride in vehicles. Among them, the suspension strut used in the suspension system is an important component that stabilizes the vehicle body by mitigating the impact caused by uneven road surfaces.&nbsp;</p><p>“JASO C611 Suspension Struts for Automobiles”1), a standard of JASO (Japanese Automotive Standards Organization), established by JSAE, specifies the definition, quality, and test method for suspension struts. There is no international standard that is equivalent to JASO C611.&nbsp;</p><p>In order to improve the performance of suspension struts, it is necessary to measure the damping force against speed by changing the excitation speed. The performance of suspension struts can be confirmed by measuring the response, such as the relationship between speed and damping force and the Lissajous waveform.&nbsp;</p><p>This article introduces an example of the test method “Damping Force Characteristic Test” specified in JASO C611, which was conducted on a Shimadzu EMT series electromagnetic fatigue testing machine.</p><h4><strong>Testing Equipment&nbsp;</strong></h4><p>The EMT-1kNV-50 electromagnetic dynamic and fatigue testing machine was used in this test. Fig. 1 shows a photograph of the testing machine. The electromagnetic actuator with extremely high frequency response enables highly accurate dynamic testing. In this test, the maximum test speed was 0.6 m/s. The EMT seriesis most suitable for testing at such high frequencies.</p><h4><strong>Conclusion&nbsp;</strong></h4><p>A damping force characteristic test on a suspension strut based on JASO C611 was conducted using an EMT series electromagnetic fatigue testing machine and Windows software for 4830. The test conditions were set using the excitation speed, and it was confirmed that the test was accurately controlled as set. The standard “JASO C611 Suspension Struts for Automobiles” referred to this time is derived from “JASO C602 Automotive Parts - Telescopic Shock Absorbers for Suspension Systems”2). Although C602 also prescribes a damping force characteristic test, it is almost equivalent to the C611, and the test machine configuration can be used by replacing the jig.</p><h3><strong>3. Thermo Fisher Scientific: </strong><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/products/2206"><strong>Hypulse Surface Analysis System</strong></a></h3><ul><li>Other document</li><li><a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Hypulse_DS_0516_EN_05_2025_1998238_WEB_0e44628a5e.pdf"><strong>Full PDF for download</strong></a></li></ul><h4><strong>Key features&nbsp;</strong></h4><ul><li><strong>Unique depth profiling capability</strong>. The femtosecond laser ablation system provides controlled material removal without inducing chemical damage to your sample.&nbsp;</li><li><strong>Fast, efficient XPS</strong>. Quick sample pump-down times, a unique sample viewing system, and high sensitivity for all analysis areas help ensure exceptional data quality, even for highly challenging samples.&nbsp;</li><li><strong>Co-incident additional spectroscopy</strong>. Optimize the information obtained from features of interest with the addition of optional analytical techniques, including ion scattering spectroscopy, reflected electron energy loss spectroscopy, and UV photoelectron spectroscopy.&nbsp;</li><li><strong>Fully featured software. </strong>The latest version of the Avantage Data System is included for data collection and analysis, with the ability to import data into Thermo Scientific Maps Software for correlation with electron microscopy images.</li></ul><h4><strong>Technical highlights&nbsp;</strong></h4><h5><strong>Micro-focused, monochromated aluminum K-alpha X-ray source&nbsp;</strong></h5><ul><li>Computer-controlled quartz-crystal monochromator&nbsp;</li><li>Software-adjustable spot size&nbsp;</li><li>Spot size range: 10–400 μm&nbsp;</li><li>Motorized, water-cooled anode with 16 positions&nbsp;</li></ul><h5><strong>Electron optics and analyzer</strong></h5><ul><li>Electrostatic objective lens&nbsp;</li><li>Full 180° hemispherical μ-metal shielded analyzer&nbsp;</li><li>Continuously selectable pass energy: 1–400 eV&nbsp;</li><li>128-channel, signature-corrected, positionsensitive detector&nbsp;</li><li>Automatic energy scale and transmission function calibration&nbsp;</li></ul><h5><strong>Charge compensation system&nbsp;</strong></h5><ul><li>Patented dual-beam electron and ion source&nbsp;</li><li>Beam energy for charge compensation: 0–5 eV&nbsp;</li></ul><h5><strong>Femtosecond-laser ablation system&nbsp;</strong></h5><ul><li>Fully integrated 1,030 nm pulsed femtosecond-laser source&nbsp;</li><li>Class 1 laser system&nbsp;</li><li>Tunable pulse energy up to 1 mJ&nbsp;</li><li>Computer-controlled attenuation optimizes ablation conditions&nbsp;</li><li>Beam imaging module for system calibration and alignment</li></ul>

News from LabRulezICPMS Library - Week 26, 2026

<p><strong>This study describes the development and validation of a dual-column, dual-detector HS-GC-FID/FID method for the forensic analysis of ethanol and other low-molecular-weight volatile compounds in biological samples. The method demonstrated excellent linearity, low detection limits, high precision, and accuracy, and was successfully verified through international proficiency testing.</strong></p><p>In addition to routine forensic application, the study reviews current practices in forensic alcohol analysis and highlights the lack of globally harmonized analytical standards. The authors propose essential methodological recommendations to improve the reliability and evidential value of ethanol determination in forensic toxicology.</p><blockquote><h4><strong>The original article</strong></h4><h4><a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80"><strong>Dual-Column HS-GC-FID/FID Method for In-Depth Analysis of Low-Molecular-Weight Volatile Alcohols in Postmortem Biological Material&nbsp;</strong></a></h4><p>Paweł Szpot, Olga Wachełko, Kaja Tusiewicz and Marcin Zawadzki</p><p>J. Xenobiot. 2026, 16(3), 80</p><p><a target="_blank" rel="noopener noreferrer" href="https://doi.org/10.3390/jox16030080">https://doi.org/10.3390/jox16030080</a></p><h6>licensed under <a target="_blank" rel="noopener noreferrer" href="https://creativecommons.org/licenses/by/4.0/"><strong>CC-BY 4.0</strong></a></h6></blockquote><p><strong>Selected sections from the article follow. Formats and hyperlinks were adapted from the original.</strong></p><div><p>The determination of low-molecular-weight volatile alcohols in <span>postmortem</span> material using headspace gas chromatography (HS-GC) is routinely performed in forensic laboratories. These analyses are important not only for law enforcement agencies in the broad sense, but also for insurance companies that decide on the payment of compensation under insurance policies. Typically, routine determination of alcohols such as methanol, ethanol, isopropanol, n-propanol, and n-butanol is sufficient to establish whether death occurred as a result of poisoning with these substances. In forensic toxicology practice, however, there are also situations in which the determination of additional chemical compounds in biological material proves to be essential. One such compound is acetone, the determination of which is crucial not only in cases of acetone or isopropanol poisoning (since acetone is a metabolite of the aforementioned alcohol) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B1-jox-16-00080">1</a>], but also in the diagnosis of ketosis caused by diabetes, hypothermia, or starvation [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B2-jox-16-00080">2</a>]. Criminal cases can be complex, and therefore, to achieve a better understanding of the circumstances preceding death, toxicological analyses are sometimes extended to also include other compounds, such as acetaldehyde [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B3-jox-16-00080">3</a>].</p><p><span>Postmortem</span> blood toxicological analysis is of particular importance because it plays a key role in determining the cause of death, especially in cases of unexplained sudden death and deaths occurring under suspicious circumstances. Even a simple screening for volatile chemical compounds can, at an early stage, allow determination of whether death resulted from poisoning or was a consequence of an underlying disease suffered by the deceased (e.g., untreated diabetes) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B4-jox-16-00080">4</a>].</p><p>One of the fundamental assumptions in forensic toxicological investigations is that their results must have evidential value and withstand scrutiny in court. Only such results can be used, for example, to reconstruct the circumstances preceding death, determine the time of substance intake or the dose of a toxic agent, or assess whether the deceased was intoxicated. This is particularly important in cases of traffic accidents, occupational accidents, drownings, domestic violence, and deaths occurring during police custody. It is worth emphasizing that the interpretation of <span>postmortem</span> toxicological results requires particular caution due to the multitude of “confounding” factors, such as interindividual variability, substance stability, endogenous formation, the postmortem interval (PMI), and the circumstances of death. To avoid introducing additional variables that could further complicate interpretation, the applied analytical method must be reliable, i.e., accurate, selective, resistant to interference, and preferably verified through independent proficiency testing [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B5-jox-16-00080">5</a>].</p><p>In gas chromatographic analysis of low-molecular-weight alcohols, either direct injection or the headspace technique is employed [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B6-jox-16-00080">6</a>]. In the past, even highly invasive approaches were used, such as the direct injection of whole blood into the gas chromatograph injector [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B7-jox-16-00080">7</a>]. However, the headspace technique has become the most widely used technique for the determination of alcohols in forensic laboratories. This preference can be attributed to several well-established advantages, most notably that the method allows the sample to be analyzed with minimal or no preparatory steps, irrespective of the biological matrix (e.g., without extraction or protein precipitation). Additional benefits include practically negligible matrix effects, elimination of the need for inlet liners, thereby minimizing the risk of contamination or carryover within the analytical system, and reduced deterioration of the chromatographic column compared with direct injection.</p><p>However, it should be noted that in the context of postmortem samples (particularly those collected from putrefied cadavers), the impact of decomposition on analyte partitioning cannot be precisely assessed. While headspace analysis generally exhibits negligible matrix effects, pre-death specimens from the same individual are rarely available for direct comparison, making it difficult to determine the extent to which postmortem changes might influence measurements. Moreover, the blood samples are subjected to multiple dilutions with an internal standard, which further minimizes potential matrix effects.</p><p>Despite numerous established methods for the determination of volatile alcohols in postmortem biological samples, current standard approaches often face limitations that can compromise forensic reliability. Single-column analyses may be prone to co-elution and interferences from structurally similar compounds, while reliance on a single detector increases the risk of undetected fluctuations and errors. Additionally, inappropriate selection of internal standards and postmortem formation of endogenous alcohols and other volatile compounds can further bias quantification. These issues highlight a critical gap, as widely adopted methods that integrate redundant analytical controls with validated procedures to minimize errors and ensure evidential reliability remain limited.</p><p>The aim of this study was to develop a method for the determination of methanol, acetone, ethanol, n-propanol, isopropanol, and n-butanol using headspace gas chromatography with dual columns and dual-flame ionization detectors. The practical applicability of the method was demonstrated using authentic forensic samples, including putrefaction fluid and antemortem blood. This study addresses the aforementioned gap by employing a dual-column/dual-FID approach, which allows cross-verification across two independent chromatographic conditions, thereby enhancing both the accuracy and reproducibility of forensic alcohol analysis. Furthermore, this paper aims to draw the attention of toxicologists worldwide to several critical factors that can significantly influence measured ethanol concentrations, potentially leading to legal and financial consequences, such as denial of compensation due to falsely elevated ethanol levels.</p><h4><strong>2. Materials and Methods</strong></h4><h5><strong>2.4. Apparatus</strong></h5><p>A <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=2010"><strong>Shimadzu GC-2010</strong></a> Plus AF IVD system (Kyoto, Japan), fitted with an advanced flow controller (AFC), a split/splitless (SPL) inlet, and two flame ionization detectors (FID), was employed. Samples were processed and introduced via a <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=1&amp;search=HS-20"><strong>Shimadzu HS-20 static headspace autosampler</strong></a> (Kyoto, Japan), with transfer to the GC through a single SPL inlet. The HS effluent was split equally (1:1) using a SilFlow<sup>®</sup> microfluidic platform (SHI-980-10593, Trajan, Ringwood, VIC, Australia) and directed onto two capillary columns: <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library?mainauthorItems=50&amp;search=Zebron-BAC1"><strong>Zebron-BAC1</strong></a> (30 m × 0.32 mm i.d., 1.8 μm film; Phenomenex, Torrance, CA, USA) and Zebron-BAC2 (30 m × 0.32 mm i.d., 1.2 μm film; Phenomenex, Torrance, CA, USA). Each column fed an independent FID channel, allowing concurrent acquisition from both separations. Headspace settings and chromatographic conditions for alcohol congener analysis followed those reported previously in [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B8-jox-16-00080">8</a>].</p><h4><strong>3. Results</strong></h4><p>Validation results are shown in <a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#table_body_display_jox-16-00080-t002">Table 2</a>. All presented values fall within the acceptable range for toxicological analysis of biological materials, in accordance with the recommendations of the German Society of Toxicological and Forensic Chemistry (GTFCh) [<a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#B10-jox-16-00080">10</a>]. Quality-control samples were prepared at three concentrations (low, medium, and high relative to the calibration range) by spiking blank samples. Relative error (RE) values within ±15% and precision expressed as relative standard deviation (RSD) ≤ 15% were considered acceptable, in accordance with GTFCh recommendations. The method, developed in 2019, has been subjected to international proficiency testing for ETB Ethanol in blood (ARVECON) and has consistently achieved positive results in each test. Additionally, the developed method for the determination of ethyl alcohol in blood was evaluated and achieved positive results in other proficiency tests, namely: the AXIO LGC Proficiency Testing Scheme in Drugs in blood Quantitative analyte selection, PT-TX-BLD (accreditation ISO/IEC 17043) (LGC Group Limited, Guildford, UK). Furthermore, the HS-GC-FID/FID method has been successfully implemented for routine toxicological analysis in our laboratory during the past 6 years.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Xenobiot_2026_16_3_80_Table_2_Validation_results_for_six_quantified_volatile_compounds_methanol_ethanol_acetone_isopropanol_n_propanol_and_n_butanol_64ddd30e8d.jpg" alt="J. Xenobiot. 2026, 16(3), 80: Table 2. Validation results for six quantified volatile compounds: methanol, ethanol, acetone, isopropanol, n-propanol, and n-butanol. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Xenobiot_2026_16_3_80_Table_2_Validation_results_for_six_quantified_volatile_compounds_methanol_ethanol_acetone_isopropanol_n_propanol_and_n_butanol_64ddd30e8d.jpg 180w," sizes="100vw" width="1238" height="1073"></p><p>Chromatograms of all compounds analyzed using the presented method, collected on two columns (Zebron ZB-BAC1 and Zebron ZB-BAC2), are shown in <a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#fig_body_display_jox-16-00080-f001">Figure 1</a>. During routine toxicological examinations, we analyzed various biological fluids, including standard matrices such as whole blood, serum, plasma, vitreous humor, and urine, as well as less commonly studied matrices such as bile, putrefaction fluid, and gastric contents.&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Xenobiot_2026_16_3_80_Figure_1_Chromatograms_of_ISTD_and_LOQ_of_volatile_compounds_obtained_from_two_columns_3826b6b870.jpg" alt="J. Xenobiot. 2026, 16(3), 80: Figure 1. Chromatograms of ISTD and LOQ of volatile compounds obtained from two columns (Zebron ZB-BAC1 and Zebron ZB-BAC2) and two flame ionization detectors. The retention time of tert-butanol (ISTD) on the first column was 2.848, and on the second it was 3.131. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Xenobiot_2026_16_3_80_Figure_1_Chromatograms_of_ISTD_and_LOQ_of_volatile_compounds_obtained_from_two_columns_3826b6b870.jpg 236w," sizes="100vw" width="1600" height="1056"></p><p>Chromatograms from representative authentic cases are shown in <a target="_blank" rel="noopener noreferrer" href="https://www.mdpi.com/2039-4713/16/3/80#fig_body_display_jox-16-00080-f002">Figure 2</a>, including putrefaction fluid (containing 0.4‰ of ethanol) compared with a standard whole blood sample containing 1.38‰ ethanol.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/J_Xenobiot_2026_16_3_80_Figure_2_A_Chromatograms_of_putrefaction_fluid_with_ethanol_concentration_of_0_4_B_chromatograms_of_antemortem_blood_sample_with_ethanol_concentration_of_1_38_fe782ce3d1.jpg" alt="J. Xenobiot. 2026, 16(3), 80: Figure 2. (A) Chromatograms of putrefaction fluid (with ethanol concentration of 0.4‰); (B) chromatograms of antemortem blood sample (with ethanol concentration of 1.38‰). Results were provided for both columns: Zebron ZB-BAC1 and Zebron ZB-BAC2." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_J_Xenobiot_2026_16_3_80_Figure_2_A_Chromatograms_of_putrefaction_fluid_with_ethanol_concentration_of_0_4_B_chromatograms_of_antemortem_blood_sample_with_ethanol_concentration_of_1_38_fe782ce3d1.jpg 245w," sizes="100vw" width="1600" height="453"></p><h4><strong>5. Conclusions</strong></h4><p>The findings presented in this study demonstrate that reliable forensic determination of ethanol and other volatile compounds requires a comprehensive analytical strategy that extends beyond routine instrumental analysis. Despite the long-standing use of alcohol determinations in forensic practice, significant methodological limitations persist, particularly with respect to selectivity, internal standard selection, and control of pre-analytical and analytical variables. Based on a critical evaluation of currently published methods and supported by experimental evidence and authentic forensic casework, several key methodological principles emerge as essential for ensuring the evidential reliability of ethanol analysis in forensic toxicology. These principles are summarized below:</p><div><ul><li>Using two columns with different polarities combined with two detectors.</li><li>Avoiding internal standards such as n-propanol, n-butanol, and other substances that are formed <span>postmortem</span> in forensic investigations.</li><li>Utilizing tert-butanol as an internal standard in forensic toxicological practice.</li><li>Analysis of quality-control (QC) concentrations defined by law for DUI threshold limits, with verification that precision and accuracy remain within acceptable ranges.</li><li>Ensuring that sample preparation is performed in facilities free from exposure to airborne organic solvent vapors, particularly methanol and ACN (which are routinely used for protein precipitation in other toxicological analyses).</li><li>Preparation of analytical batches including two replicates of each sample, analyzed in a forward- and reverse-order sequence (e.g., samples 1–5 followed by 5–1) to verify analytical consistency.</li><li>Verification of internal standards and blank samples in each batch to ensure the absence of contamination during the pre-analytical phase.</li><li>Ensuring compliance with the acceptance criterion of a maximum 5% deviation between results, based on four measurements per sample.</li><li>Careful chromatographic evaluation and critical assessment of peak shape, symmetry, and resolution to minimize the risk of undetected interferences, particularly when using detectors such as FID, where retention time is the sole discriminating parameter.</li><li>Routine performance of independent proficiency tests to assess whether the method meets required accuracy and precision standards.</li><li>Biological fluids intended for forensic toxicology testing should be collected in tubes containing sodium fluoride (NaF) and stored under controlled conditions in a refrigerator maintained at 2–8 °C.</li></ul></div></div>

Dual-Column HS-GC-FID/FID Method for In-Depth Analysis of Low-Molecular-Weight Volatile Alcohols in Postmortem Biological Material

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Electron spectroscopy for chemical analysis of liquids

Dual-Column HS-GC-FID/FID Method for In-Depth Analysis of Low-Molecular-Weight Volatile Alcohols in Postmortem Biological Material

News from LabRulezICPMS Library - Week 26, 2026

News from LabRulezLCMS Library - Week 26, 2026

News from LabRulezGCMS Library - Week 26, 2026

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