NIST26 includes integrated processing tools for LC-MS/MS data, but there are situations where working directly from instrument vendor software can be faster and more practical. When only a few peaks need to be evaluated, when mzML conversion is inconvenient, or when nominal-mass data are involved, spectra can be transferred directly into NIST Library Search for identification. This workflow can streamline spectral searching while still providing access to NIST high-resolution and low-resolution libraries, public MS/MS resources, and flexible search settings.<h4>When does direct transfer from vendor software make sense?</h4>NIST26 provides integrated data processing in the Chromatogram tab, including workflows for deconvolution and library searching. In many cases, this is the most comprehensive way to process LC-MS/MS datasets.However, chromatogram-based processing generally requires conversion of the original vendor data into mzML, for example using ProteoWizard/MSConvert. If a chromatogram is relatively simple, deconvolution is unnecessary, and only a small number of peaks need to be investigated, converting and processing the entire file may add unnecessary steps.In these situations, it can be more efficient to select an MS/MS spectrum directly in the original vendor software and send it to NIST Library Search.There are also other cases where direct transfer becomes particularly useful. Some proprietary data formats may not convert cleanly into mzML, while nominal-mass MS/MS data are not supported for MS/MS searching in the NIST26 Chromatogram tab. For this type of data, transferring or importing spectra into Library Search becomes the practical alternative.<h4>Which vendor software can send spectra directly to NIST?</h4>Several major instrument platforms provide direct integration with NIST Library Search.The workflow is available with software from:<ul><li>Agilent, including MassHunter and OpenLab,</li><li>Waters,</li><li>Thermo Fisher Scientific.</li></ul>Wiley KnowItAll can also import spectra into NIST Search and supports numerous vendor data formats through its own software environment.For some other platforms, direct NIST integration may not be available. In those situations, spectra can potentially be exported in text-based formats such as TXT or MSP ASCII and then imported into the Spectrum List in NIST Library Search.Direct transfer is generally more convenient because it avoids intermediate export and import steps.<h4>A practical Agilent example</h4>The workflow can be demonstrated using Agilent software.An analyst first selects the desired MS/MS region, averages the spectrum if necessary, and transfers it into the spectrum display. From there, the spectrum can be sent to NIST using the Search Using NIST MS Program command.Once properly configured, NIST automatically receives the spectrum and initiates the specified search. Before relying on this workflow, however, the import and library search parameters should be checked carefully.<h4>Setting up accurate-mass spectrum import</h4>For high-resolution MS/MS data, the first important step is found under Spectrum Import Options.Accurate-mass spectra should be imported with the appropriate number of decimal places for precursor and product ions, and the Accurate m/z spectrum type must be selected.Preserving mass accuracy is essential because one of the main advantages of HRMS library searching is the ability to distinguish candidates using precise precursor and fragment masses.Once the import settings are correct, the next step is configuring the library-search parameters.<h4>Searching without a precursor ion</h4>Depending on how the spectrum is selected or averaged in the vendor software, precursor information may not always be transferred automatically.NIST26 can still search such a spectrum using HiRes NoPrecursor mode.Alternatively, if the precursor m/z is known, it can be entered manually and the search can then include precursor information.The no-precursor approach can be convenient when quickly screening manually selected MS/MS spectra, particularly when the fragment pattern itself contains enough information to generate useful candidate matches.<h4>Setting mass tolerances</h4>For accurate-mass MS/MS searching, suitable precursor and product-ion tolerances are essential.An example configuration uses:<ul><li>20 ppm for the precursor ion</li><li>40 ppm for product ions</li></ul>These settings can be kept consistent with those used in the NIST26 Chromatogram workflow.Proper tolerance settings help balance search sensitivity with selectivity. If tolerances are too wide, more irrelevant candidates may be returned; if they are too narrow, valid matches can be missed because of normal measurement variation.<h4>Combining multiple spectral libraries</h4>One of the strengths of NIST Search is the ability to search multiple libraries simultaneously.A typical configuration can include the NIST high-resolution MS/MS library together with the NIST low-resolution tandem library.The NIST low-resolution library can participate in accurate-mass workflows because it contains the required indexing information. This is important because not every custom or older low-resolution library will necessarily be compatible with the same type of high-resolution search.Additional public resources can also be included, such as:<ul><li>MS-DIAL positive-mode libraries</li><li>MS-DIAL negative-mode libraries</li><li>MoNA LC-MS/MS</li></ul>Combining commercial and public libraries can substantially broaden spectral coverage. In the demonstrated workflow, several million spectra could be searched in a single configuration.For laboratories dealing with unusual metabolites, impurities, environmental contaminants, or compounds outside mainstream commercial libraries, this expanded search space can be particularly valuable.<h4>Structure searches require separate library selection</h4>MS/MS searching and structure searching do not necessarily use the same library selections.If structural searching is required, the appropriate structure-containing libraries must also be loaded in the corresponding Library Search settings.This distinction is easy to overlook when first configuring NIST26. A library may contain useful spectra but no associated structures, or the structure database may need to be explicitly selected before substructure or related structural searches can be performed.<h4>Automating the search</h4>Once the library and tolerance settings have been defined, Automatic Search can be enabled.This allows the spectrum transferred from vendor software to trigger a search immediately rather than requiring the user to launch it manually each time.For repeated workflows involving many manually selected peaks, this small automation can save considerable time.After the configuration has been optimized, it is useful to save it as a dedicated setup—for example, a configuration specifically designed for vendor-imported spectra.<h4>Understanding the result table</h4>NIST search results are typically ranked by score, but additional parameters can also help interpret the matches.Fields can include:<ul><li>score,</li><li>reverse score,</li><li>partial spectrum search score,</li><li>dot product,</li><li>molecular weight,</li><li>other user-selected parameters.</li></ul>The search result display can be customized by right-clicking the column headers and adding or removing fields.Although the standard score will usually remain the primary ranking criterion, alternative metrics can be useful when evaluating spectra with missing fragments, additional peaks, partial spectral overlap, or related structures.<h4>Why it can be useful to keep duplicate matches visible</h4>NIST includes a Best Matching Only option that reduces duplicate or repeated entries in the displayed results.When enabled, the software may reduce a list containing several library records of the same compound to the best representative match.This makes the result list easier to review, but it can also hide useful information.For initial evaluation, it is often preferable to leave this option disabled so that all matching spectra remain visible. Multiple spectra of the same compound may come from different collision energies, instruments, ion forms, or libraries, and reviewing these variations can provide useful confirmation.Filters can always be applied later once the analyst understands the full result set.<h4>Visual comparison of unknown and library spectra</h4>NIST Library Search provides several complementary views of each candidate.The experimentally acquired unknown spectrum is displayed together with the selected library spectrum, while a head-to-head spectral comparison provides an immediate visual representation of matching and non-matching ions.This is important because library-search scores should not be treated as a fully automated identification decision.A strong match should still be examined visually, especially when dealing with complex spectra, low-abundance fragments, co-isolated ions, or compounds with closely related structural analogues.The combination of numerical scoring and visual inspection provides a stronger basis for interpretation.<h4>An important issue with the Intensity Threshold setting</h4>One practical software issue deserves particular attention when importing high-resolution spectra.The Spectrum Import Options window includes an Intensity Threshold function that can remove low-intensity signals and reduce spectral noise.In principle, this would be useful. However, when an intensity threshold is applied, imported high-resolution spectra may lose their decimal mass information and be transferred as nominal-mass spectra instead.An accurate-mass peak such as 176.0815 could therefore appear simply as m/z 176.For HRMS users, this effectively removes one of the most important dimensions of the data and can compromise accurate-mass searching.Until this behavior is corrected, the safer approach is to avoid using the Intensity Threshold option during high-resolution spectrum import and perform any necessary filtering elsewhere.<h4>A related display issue in the Chromatogram window</h4>A second software behavior concerns how Spectrum Import Options are reflected in the Chromatogram tab.If the decimal precision is set to zero for importing low-resolution spectra, the head-to-head spectral display may subsequently show masses truncated according to those settings.Importantly, this is a display issue only.It does not change the accurate-mass deconvolution results or the library-search table. The underlying processed data remain unaffected; only the visual comparison at the top of the Chromatogram window reflects the altered import settings.Understanding this distinction can prevent users from mistakenly assuming that accurate-mass data have been lost from the processed results.<h4>When direct vendor-to-NIST searching is most useful</h4>Direct transfer from vendor software is not intended to replace the complete NIST26 Chromatogram workflow.Instead, it provides a complementary route for situations where full chromatographic processing is unnecessary or unavailable.It can be particularly effective when:<ul><li>only a few peaks need identification,</li><li>deconvolution is not required,</li><li>mzML conversion adds unnecessary processing,</li><li>vendor files cannot be converted reliably,</li><li>nominal-mass MS/MS spectra need to be searched,</li><li>spectra have already been reviewed and selected in the vendor environment.</li></ul>For these cases, direct spectrum transfer offers a fast route from the original MS/MS data to spectral library identification.<h4>Connecting vendor software with broader NIST26 capabilities</h4>The workflow also illustrates a broader advantage of NIST26: analysts do not have to choose between the instrument vendor's software and an independent spectral-search environment.Vendor software can remain the primary platform for inspecting chromatograms and selecting spectra, while NIST provides access to large commercial libraries, public MS/MS databases, flexible scoring, spectral comparison, and additional interpretation tools.Used together, these environments can provide a practical workflow for both rapid routine searching and more detailed unknown identification.The key is careful configuration. Accurate-mass import settings, mass tolerances, library selection, search mode, and result-display options all influence how effectively the workflow performs.Once these parameters are properly established and saved, sending a spectrum from the vendor software to NIST26 can become a rapid and repeatable part of everyday LC-MS/MS data interpretation.

Sending MS/MS (Tandem) Spectra to NIST26 Search

Compositional and Metabolomic Characterization of Major On-Farm Side Streams and Derived Products of Industrial Fine-Flavor Cocoa Processing

The integrity of sport depends on the ability to detect prohibited substances accurately, reliably and with confidence. However, as doping strategies become increasingly sophisticated, the analytical demands placed on anti-doping laboratories continue to evolve.Today’s laboratories must do more than test for a static list of known compounds. They need broad analytical strategies capable of addressing hundreds of substances across multiple chemical classes, identifying emerging threats and supporting defensible confirmation and reporting of adverse analytical findings. For more than 25 years, Thermo Fisher Scientific has worked globally with World Anti-Doping Agency (WADA)-accredited laboratories to advance the analytical science supporting modern anti-doping programs.<h3>Anti-doping testing is a moving target</h3>The World Anti-Doping Agency publishes an annually updated Prohibited List that provides a global framework for testing programs. Yet the list is non-exhaustive, meaning laboratories may also need to detect structurally related compounds and substances across several categories, with similar chemical structure and biological activity. That creates a significant analytical challenge. Anti-doping laboratories must be prepared to monitor a broad range of compounds while maintaining the sensitivity, specificity, and confidence required for both initial testing and confirmation. At the same time, the landscape continues to change. Emerging substances, rapidly expanding doping strategies and new approaches to sample collection and analysis are creating multiple considerations for anti-doping laboratories to consider. Dried blood spots (DBS), for example, have gained attention as a complementary approach to traditional urine and venous blood collection supporting applications such as detecting steroid esters and monitoring peptides. High-resolution mass spectrometry is also enabling laboratories to consider both targeted analysis and retrospective interrogation of previously acquired data as scientific knowledge and regulations evolve.<h3>From screening to confident confirmation</h3>No single analytical technique can address every anti-doping challenge.With respect to chemical analysis of toxicologically relevant compounds, modern doping control laboratories employ complementary technologies—including liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS) and isotope ratio mass spectrometry (IRMS)—depending on the compounds and analytical questions involved.For initial testing, laboratories may need to efficiently screen biological samples for numerous prohibited substances spanning categories such as steroids, stimulants, metabolic modulators, diuretics, narcotics, cannabinoids and peptides.When &nbsp;an initial Testing Procedure produces a result requiring confirmation, laboratories need analytical approaches capable of supporting confident confirmation.This combination of broad screening and targeted confirmation has made mass spectrometry central to modern anti-doping science.<h3>Expanding possibilities with LC-MS</h3>High-resolution accurate-mass mass spectrometry can provide precise mass measurements while supporting comprehensive screening of known and suspected substances, while preserving information-rich data that can support investigation of emerging or previously untargeted compounds.An important advantage of high-resolution mass spectrometry is the ability to revisit previously acquired data. As new substances, metabolites or other analytical targets are identified, laboratories can potentially re-process previously acquired full scan data (and any corresponding MSn experiment) without reanalyzing the original sample. This strategy is currently used by laboratories to support global re-testing programs.&nbsp; Triple quadrupole <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?category=20&amp;manufacturerItems=3">LC-MS</a> can complement these workflows by supporting sensitive, targeted quantitation and confirmation of prohibited substances.Together, these approaches provide laboratories the flexibility needed to address today’s testing requirements while giving laboratories greater flexibility to respond to emerging analytical challenges.<h3>The continuing role of GC-MS/MS</h3>Gas chromatography coupled with triple quadrupole mass spectrometry remains an important technology in anti-doing laboratories, particularly for urinary steroid profiling and the screening and confirmation of anabolic- &nbsp;androgenic steroids and other GC-amenable prohibited substances.GC-MS/MS can support both screening and confirmation while providing the sensitivity, selectivity and dymanic range required for screening and confirmatory analysis.The <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/485">Thermo Scientific TSQ 9610 Triple Quadrupole GC-MS</a> is designed to maximize sample throughput, helping laboratories address the demands of routine testing while maintaining analytical performance across a wide dynamic range. &nbsp;The TSQ 9610 incorporates NeverVent technology, designed to increase instrument uptime by reducing the disruption associated with routine source maintenance.As anti-doping workflows evolve, bridging screening and confirmation together within robust analytical strategies can help laboratories respond efficiently to increasingly complex testing requirements.<h3>When identifying a steroid isn’t enough</h3>In some anti-doping investigations, simply detecting a compound does not provide the complete answer.Testosterone, for example, can be produced naturally by the body or administered externally as an anabolic steroid. In cases where laboratories need to distinguish between endogenous and exogenous sources, isotope ratio mass spectrometry can provide another layer of information.GC-IRMS can help laboratories determine whether selected urinary steroids are consistent with an endogenous or exogenous origin by comparing carbon isotope ratios.The result is an analytical toolkit in which different technologies answer different questions—from broad screening and sensitive confirmation to determining the origin of a compound.<h3>Putting advanced workflows into practice</h3>Technology is only part of the equation. Real-world anti-doping laboratories also need to manage high sample volumes, turnaround expectations and continually changing analytical requirements.At Laboratoire Anti-Dopage Français (LADF), Director Dr. Magnus Ericsson and his team use advanced mass spectrometry workflows to support anti-doping testing.According to Ericsson, integration of <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=20&amp;manufacturerItems=3">Thermo Scientific TSQ</a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=37&amp;manufacturerItems=3&amp;search=vanquish">Vanquish systems</a> has helped enhance efficiency and turnaround while supporting more than 40,000 sample analyses since 2023.Experiences like these demonstrate how integrated analytical workflows can help laboratories translate advances in mass spectrometry into routine anti-doping operations.<h3>Preparing for what comes next</h3>Anti-doping science has advanced dramatically—from early testing approaches to today’s sophisticated, — mass spectrometry technologies; including but not limited to <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/products?category=20&amp;manufacturerItems=3">GC-MS/MS</a>, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?category=20&amp;manufacturerItems=3">LC-MS/MS</a>, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products?category=22&amp;manufacturerItems=3">high-resolution accurate-mass MS</a> and<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/products?category=351&amp;manufacturerItems=3"> isotope ratio MS</a> workflows.But the science cannot stand still.Emerging compounds, evolving regulations and increasingly sophisticated doping strategies mean laboratories must continually evaluate how they manage and process samples and then screen, identify, quantify and confirm prohibited substances. The goal remains constant: provide accurate, high-quality analytical results that help protect the integrity of competition.Explore the technologies, workflows and expert perspectives shaping the next generation of anti-doping testing in our comprehensive eBook, Upholding the Integrity of Sport.<blockquote><h5><a target="_blank" rel="noopener noreferrer" href="https://documents.thermofisher.com/TFS-Assets/CMD/brochures/eb-004467-cl-cmd-ms-antidoping-eb004467-na-en.pdf">Download the anti-doping eBook</a></h5></blockquote>Visit us on&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/">LinkedIn:</a>&nbsp;#AntiDoping #MassSpectrometry #SportsIntegrity #WADA

Advancing Anti-Doping Science: Analytical Strategies for the Evolving Fight Against Doping

Article

Product

Application

Academy

Video

Food

Scientific article

Science and research

Software

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

Thermo Fisher Scientific

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

My main interest is the identification of organic compounds by mass spectrometry in organic mixtures.

James Little/Mass Spec Interpretation Services

SIELC Alltesta Gradient Automated HPLC Analyzer

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system with for measuring small and trace concentrations of various chemicals.

SIELC’s Alltesta™ Analyzer is an affordable HPLC analytical system that provides both scientific and educational environments with a convenient, simple solution for measuring small and trace concentrations of various chemicals in products such as milk, baby formula, pet food, pills, household formulations, and more.No difficult sample preparation is necessary, and no prior experience in chromatography or analytical chemistry is required for the operator. Measurements typically take less than 5 minutes, and the Alltesta™ comes with a set of supplies sufficient for 200 analyses.<ul><li>Small foot-print, light-weight, can be used on a small table</li><li>No difficult sample preparation</li><li>Takes only a few minutes to start operating</li><li>No chemical experience is required for instrument user</li><li>User friendly, self explanatory cloud-based software</li><li>Produces easy-to-understand results within 5-10 minutes</li></ul><h4>Alltesta Gradient Automated HPLC Analyzer includes:</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1249">Alltesta™ Power Tower</a> (provides power and cloud connectivity)</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1244">Alltesta Mini Autosampler</a> (includes <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1245">Alltesta High-Pressure Switching Valve</a>)</li><li>Two <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1247">Alltesta™ Ultra High Pressure Mini Syringe Pumps</a> for gradient flow</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1248">Alltesta UV-Vis Detector</a></li></ul><h4>Examples of Alltesta™-based HPLC methods</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Aspirin_in_Alka_Seltzer_Extra_Strength_Effervescent_Aspirin_on_Primesep_100_Column_d33816ae83.pdf">HPLC Method for Analysis of Aspirin in Alka-Seltzer Extra Strength Effervescent Aspirin</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf">HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf">HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</a></li></ul>See this cloud-based example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation method <a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/11972/injection/21307/?t=a992fefcdd3647b19f6bed81e182d3d8">here</a>.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_SIELC_Alltesta_Gradient_Automated_Analyzer_example_of_the_2_Naphthalenesulfonic_acid_Allura_red_Fast_Green_FCF_1_5_Naphthalenedisulfonic_acid_Tartrazine_separation_b96e648d63.jpg" alt="SIELC: SIELC Alltesta™ Gradient Automated Analyzer - example of the 2-Naphthalenesulfonic acid, Allura red, Fast Green FCF, 1,5-Naphthalenedisulfonic acid, Tartrazine separation.">

SIELC Alltesta Cromite HPLC Analyzer

Compact and cloud-connected, the Cromite™ HPLC System delivers precise UV/Vis analysis with innovative single-ended column technology—ideal for research, QC, and education labs.

The Cromite™ HPLC System from SIELC Technologies is a compact, high-performance liquid chromatography platform engineered for research, teaching, and routine quality control. It combines professional analytical performance with intuitive operation in a lightweight, space-saving design.Designed for academic institutions, research laboratories, and QC environments in pharmaceuticals, food science, and environmental analysis, Cromite™ delivers reliable separations without the complexity of conventional full-scale systems. Its automated single-vial injection, isocratic syringe pump, and UV/Vis detection enable accurate analysis of compounds such as caffeine, vitamins, food dyes, environmental contaminants, and active pharmaceutical ingredients.Cloud-enabled control software allows users to create methods, run analyses, review chromatograms, and share data remotely from virtually any device. This connectivity makes Cromite™ well suited for distributed teams, teaching environments, and flexible laboratory setups.A key innovation is SIELC’s proprietary Single-Ended HPLC Column technology (up to 150 mm length). This design eliminates tubing and fittings, minimizes dead volume, and enables tool-free column replacement, reducing setup time and the risk of leaks. Each system is supplied with a single-ended column of choice and all essential accessories for immediate operation.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_Alltesta_Cromite_HPLC_analyzator_285f593640.jpg" alt="SIELC: Alltesta Cromite HPLC analyzer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_Alltesta_Cromite_HPLC_analyzator_285f593640.jpg 234w," sizes="100vw" width="1536" height="1024"><h4>Key Features</h4><ul><li>Compact and Lightweight Design: Weighing only 1.45 kg and measuring 115 × 190 × 115 mm, Cromite™ fits easily into space-limited labs and classrooms.</li><li>Automated Injection: Single-vial automated injection simplifies operation and improves reproducibility.</li><li>Isocratic Syringe Pump: Flow rates from 0.1 to 4.0 mL/min with pressures up to 4000 psi ensure effective separation of complex mixtures.</li><li>UV/Vis Detection<ul><li>Standard wavelengths: 275, 460, 520, 630 nm</li><li>Custom configuration: any four wavelengths starting from 235 nm</li><li>Low noise (&lt;0.05 mAU) and 20 Hz detection rate support reliable quantification.</li></ul></li><li>Cloud-Based Software: Wi-Fi, Ethernet, and mobile connectivity enable remote method development, data analysis, and collaboration.</li><li>Single-Ended Column Technology<ul><li>Zero dead volume design</li><li>No tubing or fittings</li><li>Tool-free replacement</li><li>Supports 4.6, 3.2, and 2.1 mm ID columns up to 150 mm length</li></ul></li></ul><blockquote>We offer a special 15% discount for schools.</blockquote><h4>Examples of Alltesta™-based methods</h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12579/injection/22619?t=3f67e3c308cc479085c836cc682e8f1a">HPLC Method of Analysis of Ascorbic Acid</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12533/injection/22589/?t=43cad31c0e924440a18874658565c3db">HPLC Method of Analysis of Theobromine, Theophylline, Caffeine and C7</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://chrom.app/share/#/samples/12411/injection/22415/?t=0c93f4eb855c43aebcfd4c3b1fd55ce9">HPLC Method of Analysys of Aspirine</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Phenylephrine_Hydrochloride_1_in_Equate_Nasal_Spray_on_Primesep_100_Column_02f2983c25.pdf">HPLC Method for Analysis of Phenylephrine Hydrochloride 1% in Equate – Nasal Spray</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/HPLC_Method_for_Analysis_of_Rockstar_Zero_Carbs_Energy_Drink_with_Caffeine_and_Taurine_on_Primesep_100_Column_c87a85a0b4.pdf">HPLC Method for Analysis of Rockstar Zero Carbs Energy Drink with Caffeine and Taurine</a></li></ul><h4>Specifications</h4><ul><li>Injection: Automatic, 1 vial</li><li>Pump: Syringe, isocratic</li><li>Flow Rate: 0.1–4.0 mL/min</li><li>Maximum Pressure: 4000 psi</li><li>Noise Level: &lt;0.05 mAU</li><li>Detection Rate: 20 Hz</li><li>Wavelengths (standard): 275, 460, 520, 630 nm</li><li>Wavelengths (custom): Any four starting from 235 nm</li><li>Supported Columns: Single-Ended HPLC Columns (4.6, 3.2, 2.1 mm ID)</li><li>Column Length: ≤150 mm</li><li>Connectivity: Wi-Fi, Ethernet, Mobile network</li><li>Power Supply: 100–240 V, 50–60 Hz</li><li>Operating Temperature: 0–40 °C</li><li>Dimensions (W × H × L): 115 × 190 × 115 mm</li><li>Weight: 1.45 kg</li></ul><h4>Typical Applications</h4><ul><li>Pharmaceutical Analysis – API identification, formulation testing, and stability studies</li><li>Environmental Testing – Detection of contaminants in water and soil</li><li>Food &amp; Beverage – Analysis of vitamins, additives, and preservatives</li><li>Academic Laboratories – Hands-on chromatographic training and research</li><li>Materials &amp; Chemical Research – Evaluation of additives, catalysts, and specialty chemicals</li></ul><blockquote>For pricing information, please contact: <a>alltesta@sielc.com</a></blockquote>

KNAUER AZURA Educational 400 bar HPLC System

Based on our KNAUER AZURA Compact HPLC: Robust, modern and cost-effective.

We would like to support you in your efforts to teach chromatography in theory and practice with the KNAUER AZURA Educational System. Our special package for training includes:<ul><li>KNAUER AZURA Compact HPLC as a complete solution: an isocratic HPLC system with manual injection valve, pump, HPLC column and UV detector as well as eluent tray and solvent bottles.</li><li>Intuitive and up-to-date: The clearly defined layout helps to understand the different functions of the HPLC system. All important components are easy to access and maintain.&nbsp;</li><li>A laptop and the HPLC software ClarityChrom® to control the system and analyze results. Alternatively, KNAUER´s Mobile Control can also be used.</li><li>Training material: flow chart for installation and capillary connection, detailed application note and video tutorials.</li><li>Beyond the detailled described example application, many other easy applications are also possible on this system</li></ul>If you are more interested in a fully equipped HPLC system for daily routine analyses, we recommend the KNAUER AZURA HPLC 862 bar systems.<h3>Specifications</h3><ul><li>Back pressure range: 400 bar max.</li><li>Flow rate range: 0.001–10 ml/min</li><li>Recommended continuous flow rate: 0.1–4 ml/min</li><li>Available pump systems: Isocratic</li><li>Injection options:&nbsp;Manual, optionally automatted via electrial injection valve</li><li>Injection volume rangev:&nbsp;1 µl–5000 µl depending on sample loop and injection syringe</li><li>Column thermostatting range: Ambient</li><li>Choice of detectors:&nbsp;UVD</li><li>Choice of software packages: ClarityChrom® CDS, Mobile Control</li></ul><h3>System components</h3><div><div>By choosing the most suitable component options for your specific application task, the AZURA system adapts to your needs.</div></div><h4>Isocratic pump system</h4>The AZURA® P 4.1S isocratic compact pump is the easiest solution and can be applied, whenever an isocratic run is needed. It features an integrated pressure sensor, which ensures pressure-independent flow rate accuracy.&nbsp;Choose between specialized pump heads for reversed phase and normal phase applications.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_Educational_HPLC_System_Isocratic_pump_system_6cc15acad6.jpg" alt="Knauer: KNAUER AZURA Educational HPLC System - Isocratic pump system" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_Educational_HPLC_System_Isocratic_pump_system_6cc15acad6.jpg 245w," sizes="100vw" width="1415" height="764"><h4>Sample Injection</h4><h5>Manual injection valve</h5>KNAUER Manual injection valves V 4.1 are the easiest and most cost- effective way to inject your sample. Equippable with different sample loops, the manual injection valve can be used for a wide range of injection volumes.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_Educational_HPLC_System_Manual_injection_valve_ff921b5148.jpg" alt="Knauer: KNAUER AZURA Educational HPLC System - Manual injection valve" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_Educational_HPLC_System_Manual_injection_valve_ff921b5148.jpg 245w," sizes="100vw" width="1309" height="541"><h5>Electrically driven injection valve</h5>Whenever you need higher reproducibility and want to get user-independent results, a manual injection valve can be upgraded to an electrically switching injection valve using the KNAUER smart valve drive VU 4.1. The sample can still be applied manually, but the injection into the HPLC system is automated.For the KNAUER AZURA 400 bar Compact systems, it can be installed&nbsp;as a module in the ASM 2.2L.<h4>Column selection and thermostat</h4>Optionally available for the KNAUER AZURA Educational system. The forced air column thermostat AZURA CT 2.1 is capable of heating or cooling from 5 to 85 °C. The powerful fan and robust peltier element keep the column at a very stable temperature, thus allowing reproducible analysis results.&nbsp;Optional eluent preheating cartridges can be installed.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_Educational_HPLC_System_Column_selection_and_thermostat_567c35ab61.jpg" alt="Knauer: KNAUER AZURA Educational HPLC System - Column selection and thermostat" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_Educational_HPLC_System_Column_selection_and_thermostat_567c35ab61.jpg 245w," sizes="100vw" width="1700" height="881"><h4>Detection</h4><h5>UV/VIS detectors with single variable wavelength</h5>The AZURA UVD 2.1S is a highly competitive single variable wavelength UV detector for HPLC. It offers excellent technical specifications for routine laboratory work.&nbsp;The installed deuterium lamp covers a wavelength range from 190 to 500 nm.&nbsp;Due to a smart design, the flow cell is easily accessible and can be changed very quickly.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_Educational_HPLC_System_Compact_variable_single_wavelength_UV_detector_for_ASM_2_2_L_71a87b3680.jpg" alt="Knauer: KNAUER AZURA Educational HPLC System - Compact variable single wavelength UV detector for ASM 2.2L" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_Educational_HPLC_System_Compact_variable_single_wavelength_UV_detector_for_ASM_2_2_L_71a87b3680.jpg 243w," sizes="100vw" width="1199" height="769"><h4>Software solutions</h4><h5>Mobile Control</h5>The Mobile Control is an intuitive and cost-effective software solution for controlling and monitoring your AZURA devices and systems. The clear system overview provides instant information on key parameters and system status. Adjust and operate single devices or even whole systems with a few fingertips.&nbsp;The well-structured block programming assists you to clearly and quickly automate device control. A smart user management makes the Mobile Control safe. Device parameters can only be changed by authorized operators.<img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_KNAUER_AZURA_Educational_HPLC_System_Mobile_Control_software_2052b3ed77.jpg" alt="Knauer: KNAUER AZURA Educational HPLC System - Mobile Control software" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_KNAUER_AZURA_Educational_HPLC_System_Mobile_Control_software_2052b3ed77.jpg 241w," sizes="100vw" width="1280" height="830"><h5>ClarityChrom® CDS</h5>ClarityChrom is an easy-to-use chromatography data system for workstations. The optional control extensions for GC, PDA and GPC allow to use the software for a wide range of applications.&nbsp;

Products from category Compact HPLC focused on HPLC - page 1

Related content

Advancing Anti-Doping Science: Analytical Strategies for the Evolving Fight Against Doping

Sending MS/MS (Tandem) Spectra to NIST26 Search

Compositional and Metabolomic Characterization of Major On-Farm Side Streams and Derived Products of Industrial Fine-Flavor Cocoa Processing