<p><strong>Gas chromatography (GC) is a powerful analytical technique used to separate and analyze compounds in a mixture. Whether you're working in pharmaceuticals, environmental testing, or food safety, understanding the key components of a gas chromatograph is essential for accurate and efficient results.</strong></p><p>In this article, we will delve into the primary components of gas chromatography, including the injector, column, detector, and carrier gas—and explain how each plays a critical role in the separation and analysis process.</p><p>By mastering these elements, you can optimize your GC setup for precise and reliable outcomes. Let's explore each component in detail:</p><h3><strong>Sample Injector</strong></h3><p>The injector is the starting point in the <a target="_blank" rel="noopener noreferrer" href="https://www.phenomenex.com/techniques/gc"><strong>gas chromatography process</strong></a>, where the sample is introduced into the system. In GC, the sample must be vaporized into a gas before it enters the column. The injector serves this purpose by heating the sample to its gaseous state. There are many types of injectors, Split/Splitless, PTV, on column.</p><p>The most common injector is the Split/Splitless injector that can be used in split mode or in splitless mode:</p><ul><li><strong>Split Injection:</strong> This mode allows a portion of the sample to be sent to the column while the remainder is vented. It is useful for high-concentration samples to prevent system overload.</li><li><strong>Splitless Injection:</strong> In this mode, the entire sample is introduced into the column, making it ideal for trace analysis where even minute quantities need detection.</li></ul><p>Proper choice of injector type and proper control of injection conditions, such as temperature, sample size, injection mode are crucial for achieving reproducible and reliable results.</p><h3><strong>Carrier Gas</strong></h3><p>In gas chromatography, the mobile phase is represented by carrier gas, which transports the vaporized sample through the column. The carrier gas is an inert gas, such as helium, nitrogen, or hydrogen. The choice of carrier gas can influence the efficiency of the separation process.</p><ul><li><strong>Helium</strong> is the most used carrier gas due to its inertness and high flow rate.</li><li><strong>Hydrogen</strong> offers faster analysis times but requires careful handling due to its flammability.</li><li><strong>Nitrogen</strong> provides a cheaper solution compared to Helium, although it's slower than helium or hydrogen.</li></ul><p>The flow rate and pressure of the carrier gas need to be carefully controlled to ensure that the sample moves smoothly through the system and adequate efficiency is generated.</p><h3><strong>Column/Stationary Phase</strong></h3><p>The column is the heart of the gas chromatograph, where the actual separation of compounds occurs. <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/gcms/products?category=7&amp;manufacturerItems=50"><strong>GC columns</strong></a> are typically long, thin tubes made from stainless steel or fused silica. They are coated with a stationary phase, which interacts with the components of the sample as they pass through.</p><ul><li><strong>Packed Columns:</strong> These contain small particles of stationary phase material and generally have a larger volume. While they are less efficient than capillary columns, they can be suitable for specific applications.</li><li><strong>Capillary Columns:</strong> These thin tubes are coated with a stationary phase, offering higher resolution and faster analysis times due to their smaller diameter.</li></ul><p>The length, diameter, and type of stationary phase of the column are important factors that affect the efficiency and selectivity of the separation. The choice of stationary phase (polar or non-polar; liquid-coated or solid) affects how different compounds interact with it.</p><p>As the sample moves through the column, compounds separate based on volatility and their affinity for the stationary phase, resulting in distinct peaks in the chromatogram.</p><h3><strong>Detector</strong></h3><p>Once the sample has passed through the column, it reaches the detector, where individual components are identified and measured. There are several types of detectors, each suited to different applications:</p><ul><li><span><strong>Flame Ionization Detector (FID):</strong> One of the most commonly used detectors in GC. It measures ions produced when organic compounds are burned in a hydrogen-air flame. FID is highly sensitive to a wide range of organic compounds and offers a broad linear dynamic range.</span></li><li><span><strong>Thermal Conductivity Detector (TCD):</strong> Detects changes in the thermal conductivity of the gas stream as analytes elute from the column. It is a universal, non-destructive detector, but is generally less sensitive than FID.</span></li><li><strong>Electron capture detector (ECD):</strong> A very sensitive and selective detector used to detect chlorinated compounds it is easily contaminated and requires very pure and dry carrier and makeup gases and difficult maintenance.</li><li><strong>Mass Spectrometer (MS):</strong> Provides structural information, aiding in both identification and quantification. Requires advanced and trained users and it’s difficult to maintain.</li></ul><p>The choice of detector depends on the type of analysis being performed and the sensitivity required. Each detector converts physical or chemical changes associated with the analytes into a measurable signal, producing a chromatogram that reflects concentration and retention time.</p><h3><strong>Data System</strong></h3><p>Although not a physical part of the chromatograph, the data system plays an integral role in processing and interpreting the signals generated by the detector. Modern gas chromatographs are equipped with software that converts detector signals into chromatograms—visual representations of the separation process. The software allows users to quantify and identify the components of the sample based on retention times and peak areas.</p><h3><strong>How to Read a Gas Chromatogram?</strong></h3><p>Interpreting a gas chromatogram involves understanding its essential elements, such as peaks, retention times, and the overall data for qualitative and quantitative analysis.</p><h4><strong>The Nature of the Sample</strong></h4><p>Begin by considering the sample's characteristics; different samples, such as environmental pollutants or food components, may exhibit distinct peak patterns. Recognizing the baseline (the flat line representing the signal without analytes) is crucial for identifying peaks accurately. While well-defined peaks indicate successful separation of components, poorly shaped or broad peaks may suggest issues with the separation process. For instance, if a mixture is injected and three peaks are observed, it indicates the presence of three different compounds in the sample. Conversely, when evaluating the purity of a sample, one would anticipate observing a single peak. Ideally, the chromatogram should reflect this expectation, confirming the sample's purity.</p><h4><strong>The Identity of the Sample</strong></h4><p>Retention time (Rt) is a critical factor in identifying the compounds present in the sample. Each analyte has a characteristic retention time that can be compared to known standards or literature. To confirm identities, a standard mixture should run alongside the sample; this allows to correlate retention times with specific compounds. If available, utilizing MS in conjunction with gas chromatography (GC-MS) can provide additional structural information, enhancing identification accuracy. With HR-MS or MS-MS detectors, unknown compounds can be identified by matching perfectly the Molecular weight or by matching the fragmentation pattern.</p><h4><strong>The Amount of Sample</strong></h4><p>Quantifying analytes involves analyzing peak area or height, which is directly proportional to the concentration of the analyte in the sample. A calibration curve constructed from known concentrations can help relate peak areas to actual concentrations. It is essential to consider the detector’s sensitivity and the limits of detection, as peaks below a certain threshold may not provide reliable quantification.</p>

Basic Principles of Gas Chromatography - Separation and Analysis

ISC 2026 - Day 2

<h4><strong>What if you could compress 179 days of grueling laboratory workflow into just 14 hours?</strong></h4><p><strong>In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with analytical chemist Laura Penabad to discuss her groundbreaking new research in biocatalysis and high-throughput screening. Evolving engineered enzymes typically requires checking thousands of variants—a massive bottleneck when traditional Liquid Chromatography-Mass Spectrometry (LC-MS) runs take minutes per sample. Laura reveals how her team built a modular screening platform that shatters this limitation, running samples 128 times faster than standard methods without sacrificing analytical accuracy.</strong></p><p>Laura breaks down the "orchestra" of technologies making this possible, including coupling nanoliter droplet microfluidics with Cyclic Ion Mobility-Mass Spectrometry (cIM-MS) to separate complex chemical isomers in milliseconds. She also shares the chaotic but triumphant behind-the-scenes stories of her PhD journey—from presenting a "comically bad" early poster at ASMS to recruiting an undergraduate mechanical engineer to build custom automation software from scratch.</p><p>If you are an early-career researcher, a mass spec enthusiast, or curious about the future of synthetic biology and machine learning in chemistry, this episode is packed with invaluable intuition and advice.</p><h3><strong>Video Transcription</strong></h3><h4><strong>From Months to Hours: Droplet Ion Mobility–MS for Biocatalysis Screening</strong></h4><p><strong>High-throughput screening is central to directed enzyme evolution, but conventional analytical workflows can create a difficult compromise: extremely fast screens often provide limited structural information, while techniques such as LC-MS offer much richer chemical insight at the cost of throughput. In a recent interview, analytical chemist Laura described how her work with droplet-based cyclic ion mobility mass spectrometry is helping bridge that gap—providing rapid analysis while retaining the ability to distinguish structurally similar products.</strong></p><p>Her research grew from an initially modest proof of concept into a screening platform capable of analyzing a 96-well plate in roughly ten minutes. The resulting workflow was approximately <strong>128 times faster than a conventional three-minute LC-MS analysis</strong>, opening new possibilities for directed evolution campaigns in which thousands of enzyme variants need to be evaluated.</p><h4>Directed evolution needs better analytical screening</h4><p>Biocatalysis takes advantage of enzymes as highly selective catalysts capable of performing reactions under relatively mild conditions. Directed evolution extends those capabilities by deliberately modifying enzyme sequences, expressing large numbers of variants, screening their activity, and using the best-performing mutants as starting points for subsequent rounds of evolution.</p><p>The process can be repeated multiple times, progressively steering an enzyme toward improved activity, altered substrate specificity, or even chemistry that does not occur naturally.</p><p>But the effectiveness of directed evolution depends heavily on the screening step. The more variants that can be analyzed—and the more useful chemical information that can be collected for each one—the more effectively researchers can explore the enormous sequence space available to an enzyme.</p><p>For Laura, this created a clear analytical challenge: develop a screening platform that was substantially faster than conventional chromatographic analysis without sacrificing the structural discrimination needed for chemically similar products.</p><h4><strong>From an imperfect first experiment to a working platform</strong></h4><p>The project did not begin with a polished high-throughput workflow.</p><p>Laura recalled looking back at her <strong>2023 ASMS poster</strong>, where the early system was still working with standards rather than true isomeric samples. Droplet introduction was slow—around six seconds per droplet—and the signals were far from the ideal reproducible, square, flat-topped profiles expected from replicate droplets.</p><p>Nevertheless, simply producing a droplet signal represented an important first milestone.</p><p>The decisive change came when the project stopped being treated merely as a demonstration that droplet ion mobility–MS could work and instead became a deliberate effort to build a <strong>robust screening platform</strong>.</p><p>Laura approached the problem in modules. First came reliable droplet introduction. Then the ion mobility separation method. Quantification became another independent component. Matrix effects were introduced later, gradually making the system resemble the conditions expected with real screening samples.</p><p>Each layer was tested before another was added.</p><p>This stepwise strategy became one of the central principles of the project: rather than attempting to optimize the entire system simultaneously, individual components were developed, challenged, and then assembled into an increasingly realistic workflow.</p><h4><strong>Finding the middle ground between speed and structural information</strong></h4><p>One of the biggest analytical problems in high-throughput screening is distinguishing products with the same nominal or exact mass.</p><p>Constitutional isomers may contain the same atoms and therefore generate the same mass-to-charge ratio, while differing only in how functional groups are arranged within the molecular structure. Very rapid optical or mass-based screens may therefore struggle to distinguish them.</p><p>Traditional LC-MS can solve many of these problems through chromatographic separation, but a several-minute analysis rapidly becomes impractical when thousands of enzyme variants must be screened.</p><p>Ion mobility offered Laura’s team another option.</p><p>Rather than separating compounds on a chromatographic timescale, ion mobility can differentiate ions according to their gas-phase behavior on a much shorter timescale. In the workflow described in the interview, this created a useful compromise: not the kilohertz throughput possible with some simple screening technologies, but dramatically faster structural differentiation than conventional LC-MS.</p><p>The goal was therefore not simply to achieve the fastest possible screen, but to find a <strong>middle-throughput analytical regime with enough resolving power to answer chemically meaningful questions</strong>.</p><h4><strong>A 96-well plate in about ten minutes</strong></h4><p>That strategy eventually produced one of the most striking results of the project.</p><p>The droplet cyclic ion mobility–MS platform processed a <strong>96-well plate in roughly ten minutes</strong>, while maintaining quantitative results comparable with the conventional LC-MS workflow used for comparison. According to the interview, this represented an approximately <strong>128-fold increase in analysis speed relative to a three-minute LC-MS run</strong>.</p><p>Laura described the successful plate experiment less as a surprise than as confirmation that the modular development strategy had worked.</p><p>Before reaching that point, the workflow had already been challenged through a sequence of increasingly demanding experiments: droplet formation, ion mobility separation, calibration, matrix-containing calibration, and cross-testing analytes at different concentrations. By the time the complete plate was analyzed, each individual part of the analytical system had already been tested under progressively more realistic conditions.</p><h4><strong>What does that speed mean for directed evolution?</strong></h4><p>The impact becomes even clearer when the workflow is considered at the scale of an entire directed-evolution campaign.</p><p>Laura’s manuscript estimated that a <strong>five-round campaign involving 5,000 variants would require approximately 179 days using the conventional LC-MS workflow but only about 14 hours using the new platform</strong>.</p><p>Compressing months of analytical work into less than a day has consequences beyond laboratory productivity.</p><p>It allows researchers to screen more enzyme variants and therefore sample a larger portion of sequence space. But the resulting datasets may also become increasingly valuable for computational approaches.</p><p>Rather than retaining information only about the best-performing enzyme variant, researchers can preserve data describing variants that performed poorly as well as those that succeeded. When genotype and phenotype information remain linked through the well-plate workflow, these larger datasets can potentially provide richer input for machine-learning models designed to guide subsequent rounds of enzyme evolution.</p><p>Faster analysis therefore does more than accelerate an existing experiment—it can change the scale and design of the experiment itself.</p><h4><strong>Balancing ion mobility resolution and signal</strong></h4><p>Increasing cyclic ion mobility separation time can improve resolution, but it also introduces an important trade-off: additional passes can reduce signal.</p><p>Laura optimized this balance experimentally by continuously infusing mixtures containing both isomers and progressively adjusting the ion mobility conditions. After tuning parameters such as wave height and velocity, increasing the separation to <strong>three passes</strong> provided baseline resolution at the high analyte concentrations used during method development.</p><blockquote><p><strong>Concentration, however, also mattered.</strong></p></blockquote><p>At higher ion populations, space-charge effects could broaden peaks and reduce the baseline separation. This meant that the optimal number of passes could not be selected independently of the concentration range required by the final assay.</p><p>Her broader advice for optimization is therefore application-driven: decide first what the analytical method actually needs to accomplish.</p><p>More speed will almost always require sacrificing something—resolution, signal intensity, quantitative precision, or another performance parameter. The key is to identify which compromise the application can tolerate and then optimize the system around that requirement.</p><h4><strong>Solving droplet carryover with a practical compromise</strong></h4><p>Droplet-based workflows present their own engineering challenges, including carryover between neighboring samples.</p><p>In Laura’s system, analytes showed little tendency to partition into the fluorinated oil separating aqueous droplets within the Teflon tubing. The more troublesome carryover occurred closer to electrospray ionization, where a small amount of sample could remain associated with the interface and appear when the next droplet arrived.</p><p>Rather than adding excessive engineering complexity to an already elaborate platform, the researchers adopted a pragmatic solution: a <strong>sacrificial wash droplet</strong> between analytical samples.</p><p>It is an example of a recurring theme throughout the project. The objective was not to construct the theoretically most sophisticated droplet system, but to create a screening workflow that remained sufficiently robust, reproducible, and practical for large numbers of samples.</p><h4><strong>High-throughput analysis also requires high-throughput data processing</strong></h4><p>Accelerating the mass spectrometry experiment created another bottleneck: the data.</p><p>The instrument produced a continuous intensity-versus-time trace rather than a neatly organized well-plate dataset. With small experiments, Laura could manually identify individual droplets and process the results in spreadsheets. That approach quickly became unrealistic as sample numbers increased.</p><p>The solution emerged through collaboration with an undergraduate mentee, Aiden, who helped develop a dedicated software tool for processing the continuous traces.</p><p>Laura defined how the software should behave and how the analytical information should be organized, while Aiden developed the computational implementation. The workflow itself was subsequently adjusted to make automated data processing more reliable—for example, by introducing marker columns that allowed the software to recognize the end of each plate row.</p><p>The resulting tool can accept the acquired traces together with information about plate dimensions and replicates and automatically return parameters including <strong>peak identity, duration, and the corresponding well position</strong>. A later version also generated an automatically populated heat map.</p><p>The experience illustrates how improving analytical throughput often requires simultaneous development of instrumentation, experimental design, and informatics. Making the measurement faster is useful only if the resulting data can be processed at a comparable speed.</p><h4><strong>Mentoring became part of the scientific process</strong></h4><p>The software project also reflects another theme Laura emphasized throughout the interview: the value of mentoring.</p><p>Several younger researchers contributed directly to the development of the final platform. Laura described mentees not simply as additional hands in the laboratory but as scientists capable of proposing their own hypotheses and experimental ideas.</p><p>Working with undergraduate researchers also influenced the way she organized experiments.</p><p>Because their available laboratory time was limited, each task needed to have a clear purpose. That encouraged a more disciplined approach to experimental planning: instead of performing experiments simply because they could be done, the team had to ask which experiment would most efficiently answer the next important question.</p><p>In that sense, mentoring became another mechanism for improving the research itself.</p><h4><strong>The next step: continuous, automated screening</strong></h4><p>Despite the dramatic increase in analytical speed, the current workflow still includes manual operations such as loading tubing and connecting the sample stream to the mass spectrometer.</p><p>The next engineering challenge is therefore automation.</p><p>Laura described ongoing work using the <strong>Venturi effect</strong> to pull samples directly from wells into the droplet system. Building on previous work in the laboratory with 96-well plates, she has already tested this approach with as many as <strong>2,000 samples</strong>.</p><p>The long-term direction is clear: continuous and robust droplet generation, automated handling of thousands of samples, rapid ion mobility separation, mass spectrometric detection, and automated reconstruction of the resulting data into plate-based outputs.</p><p>Combining those elements could move the system closer to the type of large-scale screening required for increasingly ambitious biocatalysis campaigns.</p><h4><strong>Learning mass spectrometry one problem at a time</strong></h4><p>Laura also reflected on the broader experience of developing the platform during her PhD.</p><p>Ion mobility and mass spectrometry initially appeared intimidating, particularly because they combine concepts from analytical chemistry, physical chemistry, separations, instrumentation, and electrical engineering. What eventually changed was not simply learning more facts, but developing the ability to move repeatedly between theory and experiment.</p><p>An unexpected result sends the researcher back to the underlying theory. A better understanding suggests a modified experiment. That experiment creates another observation, gradually building intuition about how the system behaves.</p><p>Her advice to early-career scientists is therefore to accept that this process takes time.</p><p>Advanced analytical techniques may initially appear overwhelming, but understanding develops incrementally through repeated interaction between theory, experimentation, failure, and refinement. Equally important, she emphasized the role of the supportive ion mobility–mass spectrometry community in making a technically demanding field more approachable for young researchers.</p><p>From the first inconsistent droplets to a platform capable of processing thousands of samples, that iterative mindset ultimately shaped the entire project.</p><blockquote><p><strong>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</strong></p></blockquote><blockquote><h4><strong>Concentrating on Chromatography Podcast</strong></h4><p>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!</p><p>You can find <strong>Concentrating on Chromatography Podcast </strong>in podcast apps:</p><ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL"><strong>Spotify</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185"><strong>Apple podcast</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography"><strong>podscan</strong></a></li></ul><p>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos"><strong>YouTube channel</strong></a></p></blockquote>

Droplet Cyclic Ion Mobility Mass Spec: Compressing Months of Enzyme Screening Into Just 14 Hours

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Phenomenex is a global technology leader committed to developing novel analytical chemistry solutions that solve the separation and purification challenges of researchers in industrial, government and academic laboratories.

Phenomenex

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Scientific™ ISQ™ 7000 Single Quadrupole GC-MS System

Robust single quad GC-MS with advanced ion sources, NeverVent™ tech, SmartTune, and flexible software. Delivers sensitive, high-throughput routine analysis.

<h4><strong>Unstoppable GC-MS performance for routine, regulatory, and high-throughput analysis</strong></h4><p><strong>The ISQ 7000 GC-MS system delivers reliable, scalable, and ultra-sensitive performance for routine analysis in food safety, environmental, clinical, and forensic laboratories. Designed to maximize uptime and analytical confidence, it features advanced ion source options, easy maintenance with NeverVent™ technology, and intuitive software tools like SmartTune and AutoSIM.</strong></p><h3><strong>Key Features and Benefits</strong></h3><h4>🚀 <strong>Unstoppable Sensitivity</strong></h4><ul><li>Advanced Electron Ionization (AEI) source for femtogram-level detection</li><li>Robust ExtractaBrite™ ion source, fully removable under vacuum</li><li>Dual-filament design and S-shaped ion optics reduce neutral and chemical noise</li><li>Simultaneous full-scan and SIM acquisition modes</li></ul><h4>⚙️ <strong>Unstoppable Uptime</strong></h4><ul><li><strong>NeverVent™ technology</strong> with Vacuum Probe Interlock (VPI) and V-Lock for maintenance without venting</li><li>Heated ion optics and RF lens protect quadrupole from matrix contamination</li><li>Reliable quadrupole design: solid, non-coated rods and maintenance-free performance</li><li>Up to <strong>98% downtime reduction</strong> for routine interventions (e.g. source cleaning)</li></ul><h4>🧠 <strong>Smart Automation and Ease of Use</strong></h4><ul><li><strong>SmartTune</strong> for guided, fast, and consistent system tuning</li><li><strong>AutoSIM</strong> and <strong>Timed-SIM (t-SIM)</strong> for efficient method creation</li><li><strong>Retention Time Alignment (RTA)</strong> tool for consistent results across column changes</li><li>Deconvolution tools for untargeted compound identification</li></ul><h4>🧪 <strong>Flexible Analytical Capabilities</strong></h4><ul><li>EI, PCI, NCI ionization modes (with optional dual reagent gas switching)</li><li>Mass range: 1.2–1100 u; scanning speed up to 240 scans/s</li><li>Optional Direct Insertion and Direct Exposure probes for solid samples</li><li>Software-switchable ionization modes in a single analysis</li></ul><h4>🖥️ <strong>Integrated Software and Data Handling</strong></h4><ul><li>Full control via <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/76"><strong>Chromeleon™ CDS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/77"><strong>TraceFinder™</strong></a></li><li>Preloaded application libraries and eWorkflows (e.g. US EPA, clinical, toxicology)</li><li>Auto-calibration, QA/QC tools, and compliance-ready (21 CFR Part 11)</li><li>Seamless LIMS connectivity and user-guided reporting tools</li></ul><h4>🔁 <strong>Sample Introduction Options</strong></h4><ul><li>Compatible with <strong>AI/AS 1310</strong>, <strong>TriPlus 300</strong> (HS), and <strong>TriPlus RSH</strong> autosamplers</li><li>Robotic workflows including dilution, standard addition, and derivatization</li><li>Ideal for trace analysis in complex matrices and high-throughput environments</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/application::product.product/isq-7000-trace-1310-triplus-rsh-left-1200x1200.jpg-650_s.webp" alt="Thermo Scientific™ ISQ™ 7000 Single Quadrupole GC-MS System" width="382" height="382"></p>

Thermo Scientific ISQ™ EC/EM Single Quadrupole Mass Spectrometer

Enhance your IC or LC analysis with ISQ EC/EM mass spectrometers. Enjoy fast setup, high sensitivity, and reliable quantification—even in complex samples—with easy, seamless system integration.

<h4><strong>Thermo Scientific™ ISQ™ EC Single Quadrupole Mass Spectrometer</strong></h4><p><strong>Add mass spectrometry (MS) to your IC and LC analyses for access to the valuable data that only MS can provide. The new, easy-to-use Thermo Scientific™ ISQ™ EC Single Quadrupole Mass Spectrometer seamlessly integrates with ion chromatography (IC) and high performance liquid chromatography (HPLC) systems. Both novices and experts alike can quickly master MS to gain more insights from every sample.</strong></p><h5><strong>Gain more insights</strong></h5><ul><li>Higher sensitivity and more accurate quantitation</li><li>Chromatographic peak mass confirmation, eliminating false negatives and false positives</li></ul><h5><strong>Resolve complexity</strong></h5><ul><li>Improved resolution of analytes in complex matrices</li><li>Better selectivity</li><li>Resolve co-eluting peaks using their mass-to-charge ratio</li></ul><h5><strong>Easily mastered</strong></h5><ul><li>Save time spent on user training with integrated tools to help master MS</li><li>Experience hassle-free, seamless integration with your IC or LC system</li><li>Save time using the Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Chromeleon™ Chromatography Data System (CDS) software</strong></a> for data analysis, which also has embedded control of the ISQ EC mass spectrometer</li></ul><h4><strong>Thermo Scientific™ ISQ™ EM Single Quadrupole Mass Spectrometer</strong></h4><p>Adding mass spectrometry (MS) to your liquid chromatography (LC) analyses gives you access to the valuable data that only MS can provide. The new, easy-to-use ISQ™ EM Single Quadrupole Mass Spectrometer seamlessly integrates with LC systems for reliable, robust, and easy LC-MS routine analysis with an extended mass range for more flexibility. Detect and quantify a variety of analytes by choosing to use either heated electrospray ionization (HESI) or atmospheric pressure chemical ionization (APCI). Integrated software allows both novices and experts to quickly master MS to gain more insights from every sample.</p><h5><strong>Gain more insights</strong></h5><ul><li>Extended mass range enables you to detect and quantify small and large molecules</li><li>Durable atmospheric pressure ionization (API) source with two interchangeable probes included, enabling HESI or dual HESI/APCI</li><li>Sensitive and accurate quantitation of more samples</li><li>Chromatographic peak mass confirmation to eliminate false negatives and false positives</li></ul><h5><strong>Resolve complexity</strong></h5><ul><li>Consistent response even with complex matrices</li><li>Better selectivity and sensitivity with chromatographic peak mass confirmation</li><li>Resolution of co-eluting peaks via mass-to-charge ratio</li></ul><h5><strong>Easy mastery</strong></h5><ul><li>Reduce time spent on user training with integrated tools to help master MS</li><li>Unique software translates analyte physical properties into optimal method parameters, helping novice and experts develop methods and improve productivity</li><li>Hassle-free, seamless integration with existing LC systems</li><li>Facilitate data analysis with Thermo Scientific™ <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/76"><strong>Chromeleon™ Chromatography Data System (CDS) software</strong></a>, which features embedded control of the ISQ EM mass spectrometer</li></ul><h4><strong>Key Features ISQ™ EC/EM Single Quadrupole Mass Spectrometers:</strong></h4><ul><li><strong>Mass Range:</strong>&nbsp;<ul><li><strong>EC:</strong> 10–1250 m/z with unit mass resolution</li><li><strong>EM:</strong> 10–2000 m/z with unit mass resolution</li></ul></li><li><strong>Polarity Switching:</strong> 25 ms (in HESI mode)</li><li><strong>Scan Rate:</strong> Up to 20,000 Da/sec</li><li><strong>Sensitivity:</strong><ul><li><i>HESI Positive:</i> 10 pg Reserpine, 400:1 RMS at 400 μL/min</li><li><i>HESI Negative:</i> 20 pg p-Nitrophenol, 500:1 RMS at 400 μL/min</li></ul></li><li><strong>Gas Requirements:</strong> Nitrogen (≥ 99% purity), 30 L/min @ 90–110 psig</li><li><strong>Power Supply:</strong> 100–240 V, 50/60 Hz</li><li><strong>Dimensions:</strong> 52 (H) × 42 (W) × 91 (D) cm</li><li><strong>Weight:</strong> Approx. 71 kg (157 lb)</li><li><strong>Unit Size:</strong> Each</li></ul>

Thermo Scientific ISQ™ 7610 Single Quadrupole GC-MS

Thermo Scientific ISQ™ 7610 Single Quadrupole GC-MS. Simplified operation, automated workflows, and extended dynamic range deliver consistent results from system to system in every laboratory.

<p><strong>To stay ahead, analytical testing laboratories need the ultimate confidence of a GC-MS system that easily and reliably produces trusted results, day after day. For this, you can count on the Thermo Scientific™ ISQ™ 7610 Single Quadrupole GC-MS System. Simplified operation, automated workflows, and extended dynamic range deliver consistent results from system to system in every laboratory. Thermo Scientific™ NeverVent™ technology, extended-life detector, and intelligent software eliminate unnecessary downtime to maximize sample throughput. To ensure you are ready for any analytical challenge, the system is upgradeable from entry-level to advanced configurations. Now you can take the lead with rapid return on investment (ROI) for your regulated GC-MS analyses.</strong></p><h4><strong>Unstoppable confidence in quantitative performance</strong></h4><p>Delivers femtogram-level sensitivity so you consistently meet required detection limits as you run samples, whether you run in SIM mode or Full Scan. The ISQ 7610 GC-MS features a unique ion path that neutral particles cannot navigate and a choice of an ExtractaBrite or AEI ion source, depending on your sensitivity needs. The instrument’s XLXR detector which is an electron multiplier detector has an extended lifetime and extended dynamic range. Extended dynamic range capability enables labs to combine methods allowing analysis of low- and high-concentration compounds in a single run.</p><h4><strong>The right choice for any workflow</strong></h4><p>The intuitive <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/cz/en/home/industrial/chromatography/chromatography-data-systems-cds.html">Thermo Scientific Chromeleon Chromatography Data System (CDS)</a> is available to help streamline quantitative workflows and ready-to-use Thermo Scientific eWorkflows can be downloaded from the <a target="_blank" rel="noopener noreferrer" href="https://appslab.thermofisher.com/">Thermo Scientific Appslab Library of Analytical Applications</a> to jump-start your method development. For U.S. EPA-based environmental applications, the Environmental Analysis Extension pack provides a set of GC-MS e-workflow and reporting templates.</p><h4><strong>Unstoppable instrument uptime for full-time productivity</strong></h4><p>The robust design of the ISQ 7610 GC-MS ensures that the system can work continuously to produce the highest quality results possible. High matrix tolerance minimizes routine maintenance, but when maintenance is required, the NeverVent technology available with the ExtractaBrite ion source enables source cleaning, column replacement or injector maintenance without requiring downtime to vent the instrument, so more time can be spent producing results.</p><h4><strong>Ease of use that helps you stay ahead</strong></h4><p>Automated software for instrument tuning, method development, targeted quantification and untargeted screening workflows make the ISQ 7610 GC-MS system easy to learn and enables quick transition to this system from other platforms. The ISQ 7610 Single Quadrupole GC-MS System features user-centric innovations including instrument health monitoring assists you in determining when maintenance is needed to avoid unnecessary downtime. As a result, you maximize instrument utilization, sample throughput, and ultimately ROI.</p><h4><strong>Unstoppable confidence in your future</strong></h4><p>To ensure you are ready for any analytical challenge, the ISQ 7610 Single Quadrupole GC-MS System is fully upgradable from entry-level to advanced configurations. Now you can be confident that your laboratory can adapt to new challenges using the same investments in GC-MS technology.</p><h3><span><strong>Specifications</strong></span></h3><ul><li><span><strong>Mass Range: </strong>1.2–1100 u with unit mass resolution&nbsp;</span></li><li><span><strong>Type:&nbsp;</strong>Single Quadrupole GC-MS</span></li><li><span><strong>Detectors:&nbsp;</strong>Triple off-axis Thermo Scientific™ DynaMax™ XTEND XR detection system, with off-axis 10 kV dynode, discrete dynode electron multiplier and electrometer, linear from 0 to 110 μA</span></li><li><span><strong>Modes:&nbsp;</strong>Electron Impact Ionization (EI), with Full Scan (FS), SIM, and FS/SIM, Simultaneous within the same injection</span></li><li><span><strong>Scanning Capabilities:&nbsp;</strong>Fast Quadrupole Scanning up to 20,000 U/s</span></li><li><span><strong>Source Type:</strong>&nbsp;Advanced Electron Ionization (AEI) Source for Ultra-Sensitivity and Robustness</span></li><li><span><strong>Item Description:&nbsp;</strong>Scalable GC-MS platform, fully upgradable from a base configuration to an advanced highly sensitive configuration</span></li><li><span><strong>Description:</strong>&nbsp;ISQ 7610 Large Turbo, No VPI with AEI</span></li><li><span><strong>Mass Stability:&nbsp;</strong>Better than 0.1 u/48 hours/∆T ≤2 K</span></li><li><span><strong>Dimensions:</strong>&nbsp;46 x 36 x 69 cm (18 x 14 x 27 in) (H x W x D)</span></li><li><span><strong>Mass Analyzer:&nbsp;</strong>Dual-stage mass filter with off-axis ion guide pre-filter for noise reduction and solid, homogeneous non- coated, maintenance-free quadrupole rods</span></li></ul>

Products from category Single quadrupoles from Thermo Fisher Scientific - page 1

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