Weak Cation Mixed-Mode Solid Phase Extraction (SPE)
A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa greater than 8, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.
Phenomenex: Strata-X-CW SPE
- Recommended Use: Extraction of basic compounds, particularly strong bases
Starting Method
For Bases with pKa > 8
- Column: 1 mL Methanol
- Equilibrate: 1 mL Water, pH 6-7
- Load: Diluted Sample, pH 6-7
- Wash 1: 1 mL Water, pH 6-7
- Wash 2: 1 mL Methanol (collect this fraction to analyze Neutrals/Acids)
- Elute Any Bases: 2x 500 µL 5 % Formic Acid in Methanol
- Elute Weak Bases: 2x 500 µL 5 % NH4OH in Methanol
Phenomenex: Strata X single tube
Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.
Available Formats
- 96-Well-Plate
Phenomenex: Strata X 96-well plate
- 1, 3, and 6mL Tubes
Phenomenex: Strata X 1, 3, and 6mL Tubes
- Giga Tubes (12-150mL Tubes)
Phenomenex: Strata X Giga Tubes (12-150mL Tubes)
