Normal Phase Solid Phase Extraction (SPE)
A polar phase with slight hydrophobic selectivity in reversed phase mode and moderate polar selectivity in normal phase mode.
Phenomenex: Strata-Silica (Si-1)- Surface Area (m²/g): 550, m²/g, null m²/g, 490 m²/g
- Separation Mode: Normal Phase
- Pore Size: 70
- Particle Size: 55
- Recommended Use: Extraction of polar compounds that are similar in structure
Starting Method
Strata Normal Phase Method
- Condition: IPA/DCM
- Equilibrate: Hexane
- Load: Pretreated sample
- Wash: 5 % DCM in Hexane
- Elute: 1:1 Hexane / DCM or 1:1 Hexane / IPA
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.
