Strong Hydrophobic Retention Solid Phase Extraction (SPE)
A wide-pore C18 sorbent that offers strong hydrophobic selectivity and accommodates molecules up to 75 kD in size.
Phenomenex: Strata C18-T
- Surface Area (m²/g): -
- Separation Mode: Reversed Phase
- Particle Size: 55
- Recommended Use: Wide pore for the extraction of large hydrophobic molecules (up to 75 kDa)
Starting Method
Strata Reversed Phase
- Condition: 1 mL Methanol
- Equilibrate: 1 mL DI Water
- Load: Pretreated sample
- Wash: 1 mL 5% Methanol in DI Water, dry under vacuum for 2-5 min
- Elute: 1 mL Methanol
Based on 100 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.
