Learn how free MoNA and MS-DIAL MS/MS libraries can complement NIST26, expand spectral search coverage, and support compound identification in LC-MS/MS workflows.

Explore the free NIST26 demo for integrated LC-MS/MS processing, including MS/MS deconvolution, library searching, test files, and support for NIST, MoNA, and MS-DIAL libraries.

Explore the NIST26 Identifications folder and learn how XIC traces, MS/MS searches, isotope information and intermediate “check” files support more confident LC-MS/MS identifications.

Learn how to convert LC-MS and GC-MS vendor data to mzML for NIST26 chromatogram processing using ProteoWizard MSConvert, including correct centroiding and Peak Picking settings.

Learn how manual and automated Hybrid MS/MS Searches in NIST26 use spectral similarity, mass differences, dForm, and fragmentation to investigate unknown compounds beyond exact library matches.

Learn how moving chromatogram results into the NIST Library Search window enables deeper candidate review, flexible precursor settings, and alternative spectral matching strategies.

Learn how NIST MS Interpreter connects accurate-mass MS/MS fragments with molecular structure, enabling substructure assignment, ppm evaluation, candidate modification, and improved unknown identification.

Learn how extracted ion chromatograms support LC–MS/MS identification, quantitation, isotope validation, and troubleshooting in the NIST XIC Analyzer workflow.

Learn how to recognize and search in-source fragmentation spectra in NIST26 using chromatogram results, Score (Unfiltered), and MS/MS Library Search for compound identification.

A practical guide to processing LC-MS/MS data in the NIST26 Chromatogram Window, from parameter setup and library searching to identification review and result export.