<p><strong>Post-translational modifications (PTMs) are where so much of biology’s complexity lives, but they are also some of the hardest molecular features to analyze with confidence. Phosphorylation, asymmetric dimethylarginine (ADMA), and O-GlcNAcylation (O-GlcNAc) often occur at low abundance, can be highly labile, and frequently require precise site localization to understand their biological meaning. This</strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_004377_ov_phosphorylation_ptm_etd_excedion_pro_tn004377_na_en_71f681655f.pdf"><strong> technical note </strong></a><strong>shows how the </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1958"><strong>Thermo Scientific Orbitrap Excedion Pro Hybrid Mass Spectrometer</strong></a><strong> helps address those challenges by combining fast, sensitive performance with complementary </strong><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/article/7217"><strong>HCD and EThcD fragmentation</strong></a><strong> workflows.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Phosphorylation_ADMA_and_O_Glc_N_Ac_Analysis_on_the_Orbitrap_Excedion_Pro_Mass_Spectrometer_d24a5de98e.jpg" alt="Thermo Fisher Scientific: Phosphorylation, ADMA, and O-GlcNAc Analysis on the Orbitrap Excedion Pro Mass Spectrometer" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Phosphorylation_ADMA_and_O_Glc_N_Ac_Analysis_on_the_Orbitrap_Excedion_Pro_Mass_Spectrometer_d24a5de98e.jpg 245w," sizes="100vw" width="1024" height="512"></p><p>Using enriched PTM samples and automated data-dependent acquisition, the study demonstrates that the <strong>Orbitrap Excedion Pro MS</strong> can deliver both depth and confidence across multiple PTM classes. For phosphorylation analysis, the platform identified 5,892 unique phosphosites from IMAC-enriched samples and <strong>2,941 </strong>unique phosphotyrosine sites after phosphotyrosine enrichment, highlighting strong performance for both broad and highly targeted signaling studies. Just as importantly, the data show that HCD and EThcD provide complementary value, with each fragmentation method contributing unique site identifications and helping resolve ambiguous assignments.</p><p>The note also highlights the value of EThcD for more analytically demanding PTMs. In ADMA-enriched samples, EThcD delivered more ADMA peptide-spectrum matches and more unique modified peptides than HCD, supporting improved confidence in site characterization. For O-glycopeptides, the Orbitrap Excedion Pro MS uses an intelligent HCD-product-dependent-EThcD strategy that triggers EThcD only when oxonium ions are detected, improving productivity while preserving the fragmentation detail needed for confident glycosite localization.</p><p>Overall, this technical note shows how <strong>Orbitrap Excedion Pro MS </strong>enables researchers to go deeper into PTM biology with greater sensitivity, better site confidence, and smarter acquisition. For scientists studying signaling, regulation, and disease mechanisms, it presents a powerful approach for expanding PTM coverage without compromising analytical rigor.</p><blockquote><h5><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/tn_004377_ov_phosphorylation_ptm_etd_excedion_pro_tn004377_na_en_71f681655f.pdf"><span><strong>Download the technical note</strong></span> "<strong>Advancing phosphorylation, ADMA, and O-GlcNAc PTM analysis with HCD and EThcD on the Orbitrap Excedion Pro mass spectrometer</strong>"</a></h5></blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#PTM #OrbitrapExcedionPro #glycosylation #phosphorylation</p>

Phosphorylation, ADMA, and O-GlcNAc Analysis on the Orbitrap Excedion Pro Mass Spectrometer

<h3><strong>Refractive Index (RI) Detection in HPLC – Making Invisible Compounds Visible</strong></h3><p><strong>When HPLC detectors are discussed, UV-Vis absorbance detection is often the first technique that comes to mind. Its popularity is easy to understand: it is sensitive, selective, and suitable for a wide range of compounds. But what happens when the analyte does not absorb ultraviolet or visible light?</strong></p><p>This is where the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/2429"><strong>Refractive Index Detector (RID)</strong></a> becomes particularly useful. Reliable, versatile, and often described as a “universal detector” for HPLC, RI detection can reveal compounds that remain invisible to many optical detectors. For substances such as sugars, polymers, lipids, or alcohols, it is often an important analytical option.</p><p>Let’s look at how RI detection works, where it performs best, what its main limitations are, and how to achieve stable, reproducible results.</p><h4><strong>How RI Detection Works</strong></h4><p>When light passes from one medium into another, its path changes direction, or refracts, depending on the phase velocity and angle of incidence. This phenomenon is described by <i>Snell’s law</i>, with the extent of refraction determined by the refractive index (RI) of the medium (Figure 1).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_1_Refraction_of_light_at_the_interface_between_two_media_with_different_refractive_indices_baa4aad28c.webp" alt="KNAUER: Figure 1: Refraction of light at the interface between two media with different refractive indices, where n₁ &gt; n₂. Since the velocity is lower in the second medium (v₂ &lt; v₁), the angle of refraction α₂ is less than the angle of incidence α₁." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_1_Refraction_of_light_at_the_interface_between_two_media_with_different_refractive_indices_baa4aad28c.webp 238w," sizes="100vw" width="1024" height="670"></p><p>In HPLC, an RI detector monitors <strong>small changes in the refractive index of the mobile phase as compounds elute from the column</strong>. Because each substance has its own refractive index, even relatively small changes in the composition of the column effluent can generate a measurable response.</p><p>Modern RI detectors generally use a <strong>differential optical design</strong> (Figure 2). Two optical paths are continuously compared: one passes through a <strong>reference cell</strong> containing pure mobile phase, while the other passes through a <strong>sample cell</strong> containing the column effluent, i.e. mobile phase plus analyte.</p><p>A light source, commonly an LED or tungsten lamp, illuminates both cells. Changes in refractive index alter the path of the light, and a photodiode detects the resulting deviation.</p><p>When only mobile phase is present, the two optical paths are effectively identical and no significant detector response is produced. As an analyte enters the sample cell, however, the refractive index changes. The light beam is slightly deflected, creating an <strong>optical imbalance</strong> and consequently a change in light intensity.</p><p>The detector converts this change into an electrical signal, which appears as a chromatographic peak. Because the response is related to analyte concentration, RI detection can also be used for quantitative analysis.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_2_Schematic_of_a_differential_RI_detector_with_dual_flow_cell_6299f55b17.webp" alt="KNAUER: Figure 2: Schematic of a differential RI detector with dual flow cell. The sample cell carries column effluent; the reference cell contains pure mobile phase. A light beam passes through both and forms a slit image on a photodiode. Any analyte-induced change in refractive index alters the beam’s path, shifting the image on the photodiode and generating a signal peak." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_2_Schematic_of_a_differential_RI_detector_with_dual_flow_cell_6299f55b17.webp 245w," sizes="100vw" width="1024" height="524"></p><p>For reliable measurements, the reference cell must always contain the same mobile phase as the sample cell, but without analyte. It therefore needs to be refreshed regularly with clean mobile phase, commonly using a switching valve and/or T-piece (Figure 3).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Figure_3_Flow_path_in_the_AZURA_RID_2_1_L_during_analysis_left_and_flushing_right_e6bc30acef.webp" alt="KNAUER: Figure 3: Flow path in the AZURA® RID 2.1L during analysis (left) and flushing (right). During analysis, solvent flows from the IN port through the heat exchanger and sample cell, then continues through the T piece and flush valve to the OUT port. During flushing, the valve redirects the flow through the T piece so the solvent passes a second heat exchanger and reference cell before exiting via the OUT port." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Figure_3_Flow_path_in_the_AZURA_RID_2_1_L_during_analysis_left_and_flushing_right_e6bc30acef.webp 245w," sizes="100vw" width="1024" height="410"></p><p>It is important to remember that refractive index depends on both <strong>light wavelength</strong> and <strong>medium density</strong>. The wavelength is fixed by the optical design of the detector, but density—and therefore refractive index—changes with <strong>temperature, pressure, and composition</strong>.</p><p>For this reason, RI detection is particularly sensitive to changes in operating conditions. Stable measurements depend heavily on maintaining a tightly controlled analytical environment.</p><h4><strong>Why Choose RI Detection?</strong></h4><p>RI detectors become especially valuable when other detector types cannot provide a useful response. They may be less sophisticated than some modern detection technologies, but several characteristics make them indispensable for particular applications.</p><h5><strong>Broad Applicability</strong></h5><p>An RID can detect virtually any compound that produces a change in the refractive index of the mobile phase. No UV-absorbing chromophore is required.</p><p>Because almost every dissolved substance changes refractive index to some degree, RI detection is commonly regarded as a <strong>universal detection technique</strong>. Typical analytes include sugars, alcohols, lipids, fatty acids, and polymers.</p><h5><strong>Non-Destructive Detection</strong></h5><p>RI measurement does not destroy the analyte. This makes it useful in workflows where the separated compound needs to be collected or analyzed further, including preparative chromatography.</p><p>KNAUER’s <a target="_blank" rel="noopener noreferrer" href="https://store.knauer.net/shop/add38-azura-r-rid-2-1l-highflow-preparative-refractive-index-detector-62927?category=75">AZURA® RID 2.1L for preparative applications</a>, for example, supports flow rates up to 100 mL/min.</p><h5><strong>Simple and Reliable Operation</strong></h5><p>RI detectors are suitable for aqueous and many organic mobile phases as long as the solvent composition remains constant.</p><p>Under stable isocratic conditions, they are relatively straightforward to use, robust, and require comparatively little maintenance.</p><h5><strong>Direct Detection Without Derivatization</strong></h5><p>Analytes do not need to be derivatized or labelled before RI detection. If a compound is soluble in the mobile phase and produces a sufficient difference in refractive index, it can be detected directly.</p><p>This simplifies sample preparation and reduces additional handling steps that could introduce variability or error.</p><p>These characteristics make RI detection particularly attractive for routine applications such as lactose determination in milk or polymer purity testing.</p><h5><strong>Limitations of RI Detection</strong></h5><p>Universal detection comes with several practical compromises. RI detectors require highly stable conditions and can be considerably more sensitive to environmental and system changes than other common HPLC detectors.</p><h5><strong>High Temperature Sensitivity</strong></h5><p>Refractive index is strongly influenced by temperature. For aqueous solvents, the change in refractive index with temperature is approximately dn/dT ≈ 10⁻⁴/°C.</p><p>Even small temperature fluctuations can therefore produce baseline drift that may be larger than the analytical signal itself.</p><p>Modern RI detectors use thermostated compartments and heat exchangers to stabilize the temperature of the flow cell. This improves accuracy and reproducibility, although the heat exchangers introduce additional system volume that can contribute to peak broadening compared with UV detection.</p><h5><strong>Limited Compatibility With Gradient Elution</strong></h5><p>RI detection is essentially restricted to <strong>isocratic chromatography</strong>.</p><p>Changing the composition of the mobile phase during a gradient produces a much larger change in refractive index than the analyte itself. The resulting baseline shift can easily overwhelm chromatographic peaks.</p><p>A stable RI baseline therefore requires the sample and reference cells to contain the same mobile-phase composition throughout the analysis.</p><h5><strong>Lower Sensitivity</strong></h5><p>Compared with UV detection, RI detection is generally about one to two orders of magnitude less sensitive.</p><p>Typical detection limits are in the µg–mg/mL range, making RID less suitable for trace-level analysis or impurity profiling.</p><h5><strong>Limited Pressure Tolerance</strong></h5><p>RI flow cells can tolerate only moderate backpressure. When several detectors are connected in series, the RI detector should therefore normally be positioned last.</p><h5><strong>Longer Equilibration</strong></h5><p>RID systems require time to reach thermal and optical stability. Warm-up and equilibration can take hours before a sufficiently stable baseline is obtained.</p><h5><strong>Sensitivity to Baseline Disturbances</strong></h5><p>Because the detector responds to any change in refractive index, many factors can affect the baseline.</p><p>Temperature fluctuations, unstable flow or pressure, pump pulsation, air bubbles, inadequate solvent mixing, and impurities in the mobile phase can all lead to noise, drift, or false signals.</p><p>Clean operation and stable system conditions are therefore essential.</p><h4><strong>How to Get the Best Performance From an RI Detector</strong></h4><p>Successful RI detection can largely be summarized in one word: <strong>stability</strong>.</p><p>Small variations in temperature, flow, solvent composition, or pressure can quickly become visible in the chromatographic baseline. Careful system preparation and handling can therefore make a major difference.</p><h5><strong>Maintain a Stable Temperature</strong></h5><p>Thermal equilibration should be one of the first priorities.</p><p>Allow the detector, column, and mobile phase to equilibrate fully before beginning analysis, ideally for at least 60 minutes. A column oven can help maintain stable conditions, and the column and detector should be operated at the same temperature whenever possible.</p><p>Environmental influences such as direct sunlight, air currents, or day-to-night laboratory temperature fluctuations should also be minimized.</p><h5><strong>Use Isocratic Methods</strong></h5><p>Keep the mobile-phase composition constant throughout the run.</p><p>If the separation requires gradient elution, an RI detector is generally not the appropriate choice.</p><h5><strong>Degas the Mobile Phase and Keep the System Clean</strong></h5><p>Use well-degassed, particle-free mobile phases and make sure the LC system is properly primed.</p><p>An in-line degasser can help prevent bubble formation. Tubing, solvent reservoirs, and the entire flow path should also be kept clean because even small bubbles or contaminants can generate spikes, noise, or other baseline disturbances.</p><h5><strong>Match the Sample Solvent Carefully</strong></h5><p>Whenever possible, prepare samples directly in the mobile phase or in a solvent with very similar composition.</p><p>Large differences between the injection solvent and mobile phase can produce strong refractive-index disturbances around the void volume.</p><p>Filtering samples also helps reduce the risk of blockages and protects the detector flow cell.</p><h5><strong>Keep Flow and Pressure Stable</strong></h5><p>Maintain a constant flow rate and avoid abrupt pressure changes, excessive pump pulsation, or leaks.</p><p>A restriction capillary or back-pressure regulator positioned after the flow cell may help stabilize pressure, although these measures cannot compensate for noise caused by a poorly performing pump.</p><h5><strong>Maintenance and Practical Considerations</strong></h5><p>Flush the reference cell regularly with fresh mobile phase. The mobile phase itself should also be replaced periodically to reduce microbial growth or compositional changes caused by evaporation.</p><p>When changing solvent systems, flush the instrument with mutually miscible solvents and allow sufficient time for the new system to equilibrate fully.</p><p>Where appropriate, mobile-phase recycling or a low-flow standby mode can help reduce solvent consumption and shorten restart times between analytical sequences.</p><blockquote><p>👉 <i>Pro Tip: Keep an eye on the baseline. A stable baseline is one of the best signs that the RID has equilibrated properly. Negative peaks are not necessarily an error—they simply indicate that the analyte has a lower refractive index than the mobile phase.</i></p></blockquote><h5><strong>The Bottom Line</strong></h5><p>With careful control of temperature, mobile-phase composition, pressure, and cleanliness, an RI detector can provide stable baselines and consistent quantitative data.</p><p>Keep the system clean, maintain constant operating conditions, and allow sufficient equilibration time, and even this demanding HPLC “diva” can deliver highly reliable results.</p><h4><strong>Typical Applications: Where RI Detection Performs Best</strong></h4><p>RI detection is particularly useful for substances that do not absorb UV light strongly and therefore cannot be detected effectively using conventional UV/VIS detectors.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_AI_Figure_4_Common_uses_of_RI_detection_in_HPLC_d84d8458dc.webp" alt="KNAUER / AI: Figure 4: Common uses of RI detection in HPLC" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_AI_Figure_4_Common_uses_of_RI_detection_in_HPLC_d84d8458dc.webp 245w," sizes="100vw" width="1024" height="433"></p><p>Typical analytes include sugars, alcohols, fatty acids, polymers, lipids, and other small organic molecules without strong chromophores.</p><h5><strong>Some common application areas include:</strong></h5><ul><li><strong>Carbohydrate and sugar analysis</strong>, including determination of glucose, fructose, sucrose, or lactose in food and beverages. HPLC-RI has been used for <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0195_sec_for_fingerprint_analysis_of_gummy_candies_ce5349cd95.pdf"><strong>product quality control of gummy candies</strong></a>, with further discussion available in this <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7186"><strong>related blog post</strong></a>.</li><li>KNAUER’s AZURA® RID 2.1L has also been used for the <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VFD_0194_d497a35278.pdf"><strong>simultaneous determination of sugars and organic acids in wine</strong></a>, an application explored further in the article <a target="_blank" rel="noopener noreferrer" href="https://www.knauer.net/blog/blog-9/the-hidden-science-in-your-glass-147">“The hidden science in your glass”</a>.</li><li><strong>Polymer and macromolecule characterization</strong> in GPC/SEC applications, for example when determining molecular-weight distributions. Examples include <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/4czsecf32d70a6d2yugpojtljg8rvp4vd4ur_05660bfad3.pdf"><strong>optimization of molecular-weight determination with the KNAUER AZURA® GPC/SEC system</strong></a> and <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/qpwsk0igeqca8b5bqunbjjqnwum09tucb83s_cc4dbbcec9.pdf"><strong>analysis of mucin proteins using an AZURA® SEC System</strong></a>.</li><li><strong>Bioprocessing</strong>, including monitoring fermentation products such as <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/VBS_0079_Characterization_of_fructans_in_fermentation_products_with_sec_bc8f294f0e.pdf"><strong>fructans</strong></a>.</li><li><strong>Pharmaceutical applications</strong>, for example analysis of excipients such as sugars and polyols, as well as identity and purity testing of APIs. Dextranes, which are used as carrier materials in pharmaceuticals and as thickening or moisturizing agents in cosmetics, can be <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/h2oikq51kfvn6ebxij6hs88pxupstfgdqfyd_3e4f29d933.pdf"><strong>characterized by SEC</strong></a>, as their properties depend strongly on molecular-weight distribution.</li><li><strong>Lipid and surfactant analysis</strong> for formulation development and quality control.</li><li><strong>Environmental analysis</strong>, including determination of selected organic and inorganic impurities in water samples.</li></ul><p>Overall, RI detection works best for analytes that are soluble, stable in solution, and compatible with straightforward isocratic separation conditions.</p><p>It is not the preferred solution for trace analysis, gradient chromatography, or workflows requiring direct MS coupling. Nevertheless, it remains a practical and dependable option for compounds that are difficult or impossible to monitor using UV or fluorescence detection.</p><h4><strong>Final Thoughts: A Detector That Still Deserves Attention</strong></h4><p>As analytical laboratories adopt increasingly sophisticated detection technologies, refractive index detection can appear relatively simple. Yet for many applications, that simplicity is exactly its strength.</p><p>For analytes such as sugars, alcohols, polymers, and other compounds without strong UV absorption, RID offers a direct, reliable, and non-destructive method of detection.</p><p>Its greatest challenges are its sensitivity to temperature and its incompatibility with gradient elution. Under carefully controlled isocratic conditions, however, RI detection remains a dependable workhorse for routine HPLC and GPC/SEC applications.</p><blockquote><p><i><strong>Pro tip: If your analyte seems invisible to conventional optical detection, a change of perspective may be all that is needed. RI detection could make it visible.</strong></i></p></blockquote><p>In the next article in the <strong>“Seeing is Believing – Detection Made Easy”</strong> series, we will focus on <strong>Fluorescence Detection (FLD)</strong>, a highly selective and sensitive technique for compounds that fluoresce naturally or can be derivatized to do so.</p>

Seeing is Believing – Detection Made Easy Part 4: Refractive Index Detector

News from LabRulezLCMS Library - Week 36, 2026

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Waters Xevo G2-XS QTof Quadrupole Time-of-Flight Mass Spectrometry

The Xevo® G2-XS QTof combines StepWaveTM ion optics with the XS Collision Cell, providing a significant increase in sensitivity with no reduction in selectivity.

<h4><strong>eXtreme Sensitivity, eXtraordinary Selectivity, eXceptional Solutions</strong></h4><div><p><strong>For the scientist who needs to identify, quantify and confirm the broadest range of compounds in the most complex and challenging samples.</strong></p><ul><li>Maximum robustness with no compromise in performance</li><li>Class-leading real-world quantitative sensitivity</li><li>Highest quality, most comprehensive qualitative information</li><li>Flexibility to adapt to changing needs</li><li>Accessible to experts and non-experts alike</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Xevo_G2_XS_Q_Tof_Quadrupole_Time_of_Flight_Mass_Spectrometry_1_b9f8dd32be/Waters-Xevo-G2-XS-QTof-Quadrupole-Time-of-Flight-Mass-Spectrometry-1.jpg" alt="Waters Xevo G2-XS QTof Quadrupole Time-of-Flight Mass Spectrometry." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Xevo_G2_XS_Q_Tof_Quadrupole_Time_of_Flight_Mass_Spectrometry_1_b9f8dd32be/thumbnail_Waters-Xevo-G2-XS-QTof-Quadrupole-Time-of-Flight-Mass-Spectrometry-1.jpg 173w," sizes="100vw" width="1099" height="991"></p><h3><strong>Overview</strong></h3><div><h4><strong>Maximum robustness with no compromise in performance</strong></h4><p>The trend for increasing complexity of both samples and studies can result in difficulty obtaining high quality data for every component of interest while maintaining optimum performance throughout the analysis.</p><p>The <strong>Xevo® G2-XS QTof</strong> combines <strong>StepWave<sup>TM</sup> ion optics</strong> with the <strong>XS Collision Cell</strong>, providing a significant increase in sensitivity with no reduction in selectivity.&nbsp; During long analyses, critical hardware components stay clean for longer, so results are more reproducible and the laboratory stays productive.</p><p>Meaningful information is obtained for a greater number of components across a larger population of samples than ever before, giving a more complete picture for improved understanding and more confident decision making.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Xevo_G2_XS_Q_Tof_Quadrupole_Time_of_Flight_Mass_Spectrometry_437f84bacd.jpg" alt="Waters Xevo G2-XS QTof Quadrupole Time-of-Flight Mass Spectrometry: Maximum robustness with no compromise in performance." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Xevo_G2_XS_Q_Tof_Quadrupole_Time_of_Flight_Mass_Spectrometry_437f84bacd.jpg 224w," sizes="100vw" width="900" height="628"></p><div><h4><strong>Class-leading real-world quantitative sensitivity</strong></h4><p>In targeted quantitative analysis, the greatest challenge is to detect and quantify components of interest down to the lowest possible levels, often in the presence of interferences from complex matrix.&nbsp; Large variation in the concentrations of different components further adds to this challenge.</p><p>The <strong>Xevo G2-XS QTof</strong> combines sensitivity and selectivity with the mass accuracy, dynamic range and speed provided by <strong>QuanTofTM technology</strong> and the enhanced quantitative capability of <strong>Tof-MRM data acquisition</strong>.&nbsp; The end result is class-leading quantitative performance in real everyday laboratory conditions, demonstrated by limits of detection and quantification superior to anything achieved before with high resolution MS.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Xevo_G2_XS_Q_Tof_Quadrupole_Time_of_Flight_Mass_Spectrometry_2_d805c7aa49.jpg" alt="Waters Xevo G2-XS QTof Quadrupole Time-of-Flight Mass Spectrometry: Class-leading real-world quantitative sensitivity." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Xevo_G2_XS_Q_Tof_Quadrupole_Time_of_Flight_Mass_Spectrometry_2_d805c7aa49.jpg 245w," sizes="100vw" width="900" height="312"></p><div><h4><strong>Highest quality, most comprehensive qualitative information</strong></h4><p>In addition to accurate quantitative profiles, complete understanding of a sample requires detailed and comprehensive qualitative information.</p><p><strong>MS<sup>E</sup></strong> is a simple but powerful approach that delivers accurate mass precursor and fragment ion data for every detectable component, whether chromatographically resolved or not.&nbsp; This represents the ultimate in qualitative information, and because the approach is un-targeted, the data can be interrogated again later as scientific questions evolve.</p><p><strong>FastDDA</strong> is an automated, targeted data acquisition method that provides rapid, intelligent accurate mass MS/MS capability for confirmation of known compounds or characterization of unknowns, for situations where only true MS/MS data will suffice.</p><h4><strong>Flexibility to adapt to changing needs</strong></h4><div><p>The growing emphasis on efficiency and productivity in the laboratory demands analytical systems that are not only robust and reliable, but that also have the flexibility to accomplish a wide variety of tasks today, with future-proofing for the innovations of tomorrow.&nbsp;</p><p><strong>Waters’ Universal Ion Source Architecture</strong> enables the use of a single analytical platform for many different applications and molecule types, as well as providing the option to add new functionality as requirements and priorities change.&nbsp; All source options are designed to be quickly interchangeable and ready to use within minutes, maximizing versatility and minimizing downtime.</p><div><h3><strong>Accessible to experts and non-experts alike</strong></h3><p>Traditionally the ability to achieve and maintain optimum performance from such a powerful analytical system would require a level of knowledge and experience held only by expert users.</p><p>The <strong>Xevo G2-XS QTof</strong> features <strong>IntelliStartTM technology</strong>, an intuitive user interface that automates routine tasks and ensures reproducible data of the highest quality, ensuring the full capability of the system is accessible to all.</p><p><strong>IntelliStart enables essential functions to be carried out automatically:</strong></p><ul><li>MS resolution and calibration checks</li><li>Simple setup of diverse experiments</li><li>Scheduled LC-MS system checks</li><li>Continuous system monitoring</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Xevo_G2_XS_Q_Tof_Quadrupole_Time_of_Flight_Mass_Spectrometry_3_279cf35277.jpg" alt="Waters Xevo G2-XS QTof Quadrupole Time-of-Flight Mass Spectrometry: Accessible to experts and non-experts alike." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Xevo_G2_XS_Q_Tof_Quadrupole_Time_of_Flight_Mass_Spectrometry_3_279cf35277.jpg 136w," sizes="100vw" width="900" height="1038"></p></div></div></div></div></div></div>

Waters SELECT SERIES Cyclic IMS

The SELECT SERIES Cyclic IMS combines novel cyclic ion mobility separation with state-of-the-art, high performance, time-of-flight mass spectrometry.

<div><div id="title-c6692d35e8" data-locator="title-with-text"><h3><strong>Endless possibilities. Limitless research.</strong></h3></div></div><div><div id="text-ba9d3d94da" data-locator="text"><p><strong>Developed through focused collaboration, the SELECT SERIES Cyclic IMS combines novel cyclic ion mobility separation with state-of-the-art, high performance, time-of-flight mass spectrometry, enabling leading researchers to unlock the potential in scientific discovery.</strong></p><p>The SELECT SERIES Cyclic IMS enables selection of ions by both mass and mobility, a unique functionality of <strong>Cyclic Ion Mobility (cIM)</strong> that provides unparalleled flexibility and capabilities for research studies in both academia and industry. With ion mobility and mass spectrometry, you can separate by shape as well as&nbsp;<i>m/z</i>&nbsp;for separation of isomers, measure the <strong>Collisional Cross Section (CCS)</strong> of an ion, and achieve significantly cleaner mass spectral data.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_Cyclic_IMS_Novel_Cyclic_Ion_Mobility_c_IM_Technology_13abf6dd9b.jpg" alt="Waters SELECT SERIES Cyclic IMS: Novel Cyclic Ion Mobility (cIM) Technology." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_Cyclic_IMS_Novel_Cyclic_Ion_Mobility_c_IM_Technology_13abf6dd9b.jpg 234w," sizes="100vw" width="1024" height="682"></p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-26eab4b89b" data-locator="text"><ul><li>Combine <strong>cyclic ion mobility (cIM) separation</strong> with <strong>ToF mass spectrometry</strong></li><li>Separate more than ever before with ultimate ion mobility resolution for complete confidence</li><li>Perform <strong>unique IMS<sup>n</sup> experiments</strong> for increased selectivity and advanced characterization</li><li>Enable <strong>pre-mobility mass selection</strong> and <strong>mobility selection of precursor and product ions</strong></li><li>Achieve increased experimental flexibility for solving complex problems with three distinct fragmentation regions</li></ul></div></div><div><div id="text-36ff553b5f" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>For identifying and rapidly monitoring co-eluting, isomeric metabolites in chemical reactions</p><blockquote><p class="cke-user_style-important"><strong>Video: </strong><a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/watch?v=nauwVH0L91o"><strong>Waters SELECT SERIES Cyclic IMS</strong></a></p></blockquote><div><div id="text-8469d7776e" data-locator="text"><h3 data-locator="modal-title"><strong>Discover more with ultimate ion mobility resolution</strong></h3><p>The instrument design and control software enable a range of experiments, including acquisition of Tof data, Single pass ion mobility, and the ability to scale mobility resolution to match the complexity of a sample. Ion mobility resolution increases with the length of the mobility device and therefore with the number of passes.</p><figure class="image"><img style="aspect-ratio:1024/682;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_Cyclic_IMS_IMS_resolution_increases_with_the_number_of_passes_around_the_c_IM_device_121719dbe0.jpg" alt="Waters SELECT SERIES Cyclic IMS: IMS resolution increases with the number of passes around the cIM device. Demonstrated here by the separation of reverse sequence peptides SDGRG and GRGDS. The Arrival Time Distributions (ATDs) obtained after 100 passes around the device are inset." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_Cyclic_IMS_IMS_resolution_increases_with_the_number_of_passes_around_the_c_IM_device_121719dbe0.jpg 234w," sizes="100vw" width="1024" height="682"></figure><div><div id="text-bb2fb9f91c" data-locator="text"><h3 data-locator="modal-title"><strong>Unlock the potential in scientific discovery</strong></h3><p>The unique instrument design of the SELECT SERIES Cyclic IMS&nbsp;facilitates advanced ion mobility experiments like IMS<sup>n</sup>&nbsp;isolation. IMS<sup>n</sup>&nbsp;isolation&nbsp;extends the resolution of a specific mobility range,&nbsp;<i>e.g.,</i>&nbsp;ions of a specific species. Eject ions (without acquisition) outside of the desired mobility range, leaving the remaining species in the cIM to continue the separation process. This “trimming” approach can be undertaken numerous times and can be used, for example, to increase specificity of the selected ions or to prevent “wrap-around” in the cIM where high mobility ions catch up with low mobility ions in multi-pass experiments. Multiple cyclic sequences of different numbers of passes can be defined in one, multi-pass LC-MS method, increasing productivity by reducing the need for multiple injections of the same sample.</p></div></div><figure class="image"><img style="aspect-ratio:1024/578;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_Cyclic_IMS_Investigation_into_acyl_glucuronides_via_infusion_f3bf2c67e2.jpg" alt="Waters SELECT SERIES Cyclic IMS: Investigation into acyl glucuronides via infusion. Slicing enables further IMS separation of target metabolite (blue region) of interest and other ions can be ejected. Increasing the IMS resolution reveals an additional 4-O acyl. In collaboration with Imperial College London – Ian D. Wilson." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_Cyclic_IMS_Investigation_into_acyl_glucuronides_via_infusion_f3bf2c67e2.jpg 245w," sizes="100vw" width="1024" height="578"></figure><div><div id="text-78a4dc4d5e" data-locator="text"><h3 data-locator="modal-title"><strong>Undertake advanced structural characterization</strong></h3><p><strong>IMS<sup>n</sup>&nbsp;activation</strong>&nbsp;is ideal for <strong>advanced structural characterization</strong>. During mobility separation, excise a segment of the mobility separated species and send the ions to the pre array storage device. Unwanted ions are ejected, and the stored ions are then re-injected into the array under collision induced activation/dis-sociation conditions, as required, and subsequent mobility separation performed. The product ions can then be sent to the&nbsp;Tof<i>&nbsp;</i>for detection or can undergo further rounds of mobility selection providing the unique IMS<sup>n</sup>&nbsp;capability.</p></div></div><div><div id="text-079d02ceb8" data-locator="text"><h3 data-locator="modal-title"><strong>Imaging mass spectrometry with DESI XS and Cyclic IMS</strong></h3><p>The unique combination of&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library?instrumentationItems=26&amp;mainauthorItems=5&amp;search=DESI+XS"><strong>DESI XS</strong></a><strong>&nbsp;</strong>with SELECT SERIES Cyclic IMS provides ultimate levels of specificity for <strong>MS imaging</strong> approaches. DESI XS produces a visual map of the spatial distribution of a wide range of analytes, for example small molecule drugs, metabolites, and lipids across the surface of a tissue sample, and the unique capabilities and modes of acquisition of Cyclic IMS enable comprehensive in-depth experiments to be performed.</p></div></div><div><div id="text-673d117887" data-locator="text"><h3 data-locator="modal-title"><strong>Increased sensitivity provides confidence in what you find</strong></h3><p>To complement the novel <strong>Cyclic Ion Mobility</strong> capability and for complete confidence in your data, the&nbsp;SELECT SERIES&nbsp;Cyclic IMS is equipped with the latest <strong>ToF technology</strong>. The XS transfer device maintains the fidelity of the ion mobility separation while simultaneously conditioning the ion beam for the absolute best time-of-flight performance. The new offset oa-Tof with dual gain detection system <strong>provides improved sensitivity, resolution &gt;100,000 (FWHM), reliable accurate exact mass measurements</strong> (low ppm), and <strong>improved dynamic range</strong>.</p><figure class="image"><img style="aspect-ratio:1024/682;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Mass_spectrum_highlighting_resolution_of_100_000_FWHM_on_the_7_charge_state_of_monomeric_9ff8f53204.jpg" alt="Waters SELECT SERIES Cyclic IMS: Mass spectrum highlighting resolution of >100,000 (FWHM) on the 7+ charge state of monomeric." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Mass_spectrum_highlighting_resolution_of_100_000_FWHM_on_the_7_charge_state_of_monomeric_9ff8f53204.jpg 234w," sizes="100vw" width="1024" height="682"></figure></div></div></div></div></div></div></div></div></div></div></div></div>

Waters SYNAPT XS High Resolution Mass Spectrometer

The SYNAPT XS Ion Mobility Time-of-Flight Mass Spectrometer provides ultimate flexibility, offering greater freedom of analytical choice to support scientific creativity and technical success for any application.

<div><div><h4><strong>Ultimate flexibility for deeper discovery</strong></h4></div></div><div><div><p><strong>In the arena of scientific discovery, the slow pace of advancement and escalating costs of research can prove to be a challenge. Unlike competitor systems with restricted inlet options, scan function limitations, or requiring multiple platforms, only Waters offers an all-encompassing high-performing LC-MS solution, the SYNAPT XS mass spectrometer, for ultimate flexibility and greater freedom of analytical choice – supporting scientific creativity and technical success for any application.</strong></p><ul><li><strong>Novel technology building blocks deliver cutting-edge analytical performance</strong></li><li><strong>SONAR</strong> and <strong>HDMSE</strong> provide a unique toolkit for interrogating complex mixtures</li><li><strong>Ion mobility</strong> multiplies peak capacity and selectivity of analysis</li><li><strong>CCS measurements</strong> increase security of compound identification</li><li><strong>Deeper discovery in Mass Spectrometry Imaging</strong> with<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/Full-Spectrum-Molecular-Imaging-/nav.htm?cid=135070661"><strong>Full Spectrum Molecular Imaging</strong></a> enabled by <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/DESI-XS---Desorption-Electrospray-Ionization-/nav.htm?cid=135047466"><strong>DESI XS</strong></a> and MALDI</li></ul><h4><strong>Overview</strong></h4><div><div><div><div><ul><li><strong>Serve the broadest range of applications </strong>with the most extensive range of chromatograph inlets for ultimate flexibility</li><li><strong>Attain fragment ion information for every detectable component</strong> for unambiguous confirmation of compound identity with DIA modes <strong>SONAR</strong>, MSE, and <strong>HDMSE</strong></li><li><strong>Generate rich fragmentation data</strong> from highly complex samples in all acquisition modes including DDA</li><li>Simplify and streamline the mass spectral imaging workflow and deliver multi-layered, information-rich data with multiple imaging techniques and accompanying High Definition Imaging (HDI) Software</li><li><strong>Achieve automated higher order structure determination </strong>thorough confident identification, intact mass analysis, and best-in-class reproducibility</li><li><strong>Enhance MS/MS possibilities</strong> using complementary fragmentation techniques with high resolution and accurate mass measurements</li></ul></div></div><div><div><h5><strong>Recommended Use</strong></h5><p>For deeper discovery by aligning complimentary workflows on a single <strong>Ion mobility</strong> enabled time-of-flight mass spectrometer with the flexibility of multiple ion sources and acquisition modes.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_4_696d77ce2b.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: The Waters SYNAPT XS offers flexibility and freedom of analytical choice with an extensive range of chromatographic inlets." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_4_696d77ce2b.jpg 234w," sizes="100vw" width="1024" height="682"></p><div><div><h3><strong>Cutting edge performance with novel technology</strong></h3><p>With technology building blocks inherited from the <strong>Waters Advanced Mass Spectrometry</strong> <strong>SELECT SERIES</strong>, advanced innovation is built-in, ensuring scientists using this platform are at the cutting edge of mass spectrometry while retaining the ease of use and established end-to-end workflows for which the <strong>SYNAPT </strong>is well known.</p><ul><li><strong>StepWave&nbsp;XS -&nbsp;</strong>Redesigned segmented quadrupole transfer optics provide enhanced sensitivity for challenging compounds while further improving levels of analytical robustness.</li><li><strong>Extended ToF -&nbsp;</strong>Delivers UPLC-compatible mass resolution, matrix-tolerant dynamic range, and quantitative results for the most complex samples, while offering superior specifications to previous SYNAPT models.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_9f19abb382.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: SYNAPT XS schematic." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_9f19abb382.jpg 245w," sizes="100vw" width="1024" height="546"></p></div></div><div><div><h3><strong>Interrogate complex mixtures with a unique toolkit</strong></h3><p>A complete analytical strategy requires the combination of appropriate complementary techniques to see the bigger picture. With both <strong>SONAR</strong> and IMS-based <strong>data independent acquisition</strong> (DIA) modes of operation on the SYNAPT XS, the discovery analyst is in a unique position to interrogate complex mixtures by complementary mechanisms. Both types of acquisition increase analytical <strong>peak capacity</strong>, providing ‘clean and clear’ fragmentation data, but based on different molecular properties. This provides a truly unique investigative toolbox for the in-depth interrogation of complex mixtures.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_2_9179e575a4.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: During each scan the quadrupole separates co-eluting precursor masses by scanning the mass range, and transiting them in sequence. Fragment ions from different precursors can then be recorded separately and assigned with confidence." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_2_9179e575a4.jpg 245w," sizes="100vw" width="1024" height="593"></p></div></div><div><div><h3><strong>Discover more with ion mobility and CSS measurement</strong></h3><p>Conventional mass spectrometers separate components based on m/z. The SYNAPT XS also allows separation of molecules according to<strong> their size, shape, and charge during ion mobility experiments as a function of their collisional cross section</strong> (CCS). As well as ion mobility offering an additional dimension of resolution, multiplying <strong>peak capacity</strong>, and <strong>selectivity of analysis</strong> the measurement of CCS offers additional molecular identifiers. The measured CCS of an ion can be used to help confirm an ion’s identity or investigate its structure. The inclusion of ion mobility significantly increases the extent and confidence with which a scientist can profile complex mixtures and complex molecules – dramatically enhancing sample definition.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_3_2240bff527.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: Retention and drift time principle ion mobility enabled data-independent LC-HDMSE analysis (LC-DIA-IM-MS)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_3_2240bff527.jpg 204w," sizes="100vw" width="1024" height="784"></p></div></div><div><div><h3><strong>Achieve ultra-confident structural characterization of components</strong></h3><p>Time aligned parallel (TAP) fragmentation is an acquisition mode unique to the T-Wave IMS design. It permits users to take the advantage of the <strong>TriWave configuration</strong>, which allows the pre-IMS T-Wave and post-IMS T-Wave to operate as two separate collision cells. The resultant CID-IMS-CID instrumental operation helps to provide ultra-confident structural characterization of components. <strong>TAP fragmentation</strong> provides a distinct advantage for building a complete structure, through superior fragment ion coverage, sensitivity, and accuracy compared to traditional MSⁿ or MS/MS techniques.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_5_4dd33f1ab5.jpg" alt="Waters SYNAPT XS High Resolution Mass Spectrometer: Schematic showing Time Aligned Parallel Fragmentation. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SYNAPT_XS_High_Resolution_Mass_Spectrometer_5_4dd33f1ab5.jpg 234w," sizes="100vw" width="1024" height="682"></p></div></div></div></div></div></div></div></div>

Waters BioAccord LC-MS System

The BioAccord LC-MS System enables routine biotherapeutic monitoring across development, manufacturing, and QC, with a compact design and user-friendly platform for non-MS experts.

<div><div id="title-61fa75209f" data-locator="title-with-text"><h3><strong>Routine biopharmaceutical analysis – accessible and accelerated</strong></h3></div></div><div><div id="text-73a19e9720" data-locator="text"><p><strong>Improving lab efficiency without sacrificing result quality is important but challenging. Now you can achieve high-quality biotherapeutic molecule data while maximizing efficiency with the ability to directly measure product attributes to assess product quality and process robustness.</strong></p><p><strong>The BioAccord LC-MS System</strong> is specifically designed for routine definition and monitoring of biotherapeutic attributes across biopharmaceutical process and product development, manufacturing, and QC, all within a small system footprint on a platform capable of operation by non-MS experts.&nbsp;</p><p>Analyze a broad range of product attributes using the intact molecule, subunits, peptide maps, and released glycans, all with greater sensitivity, selectivity, and dynamic range than traditional chromatographic and electrophoretic techniques.</p><ul><li>Make rapid, confident decisions with accessible, consistent high-quality data​</li><li>Ensure operational simplicity, result consistency, and lab productivity with workflow automation​</li><li>Compliant-ready platform that can be deployed and replicated from discovery through to QC.​</li><li>Achieve fast deployment, reliable operation, and a clear return on investment​</li></ul><h3><strong>Overview</strong></h3><ul><li>Compliant-ready platform ideal to deploy from discovery to quality control (QC)​</li><li>Achieve faster time to value, and consistent performance through automated set-up with SmartMS</li><li>Diagnose, and fix potential system issues before they affect data quality with intelligent health checks in SmartMS</li><li>Focus your team on what matters with a system that doesn't require LC-MS experts for routine operation</li><li>Make more informed, data-driven decisions with trustworthy LC-MS data directly available at the point of decision-making</li><li>Achieve the highest quality data in your lab, comparable to data previously available only from the complicated, and costly LC-MS systems used for product characterization</li></ul><p><strong>Recommended Use: </strong>For the rapid measurement of multiple product quality attributes during routine biopharmaceutical analysis.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_R_Da_Detector_1_c570b28cc2/Waters-ACQUITY-RDa-Detector-1.jpeg" alt="Waters BioAccord LC-MS System" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_R_Da_Detector_1_c570b28cc2/thumbnail_Waters-ACQUITY-RDa-Detector-1.jpeg 125w," sizes="100vw" width="1024" height="1274"></p><h3><strong>Accelerate decisions with less effort to collect and process high quality data</strong></h3><p>Minimize the time spent generating data and managing LC-MS performance and maximize time for actions driven by the high quality data generated with automated set-up and guided workflows.&nbsp;</p><h4><strong>Use the streamlined workflows with the BioAccord LC-MS System for:</strong></h4><ul><li>Intact mass analysis (proteins, subunits, oligonucleotides, mRNA fragments)</li><li>Peptide mapping and multi-attribute method (MAM)</li><li>Released N-glycan analysis</li><li>Oligonucleotide sequence confirmation</li><li>Impurity analysis for proteins, peptides, and oligonucleotides</li></ul><p>These workflows streamline method set-up, data acquisition and processing, and report generation to deliver the required information with minimal manual interventions.​ In addition to these core workflows, the BioAccord System also supports a range of other applications required in biotherapeutic process and product development, like cell culture media analysis, mRNA cap, and lipid nanoparticle analysis.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Accord_LC_MS_System_Visualize_your_data_with_intuitive_and_easy_to_review_layouts_9876dbe5a8.jpg" alt="Waters BioAccord LC-MS System: Visualize your data with intuitive and easy to review layouts. These results from a peptide MAM analysis show product quality attributes that are being monitored, flagging those that are out of specification." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Accord_LC_MS_System_Visualize_your_data_with_intuitive_and_easy_to_review_layouts_9876dbe5a8.jpg 245w," sizes="100vw" width="954" height="541"></p><h3><strong>Increase productivity by increasing data quality and reducing analysis failures</strong></h3><p>Non-specific adsorption of metal sensitive analytes can be unpredictable and challenging, leading to long system passivation times, large relative standard deviations (RSDs), and broad peaks that can be tough to detect and quantify.</p><p>The BioAccord LC-MS System incorporates ACQUITY Premier UPLC and Premier chemistries with MaxPeak High Performance Surfaces (HPS) Technology. These innovations maximize system readiness, decrease time to operation, and enable superior run-run and system-system consistency while improving sensitivity for the metal interacting analytes.&nbsp;</p><h4><strong>Through the application of MaxPeak HPS Technology, you can:</strong></h4><ul><li>Increase recovery and consistent detection of problematic peptides for enhanced peptide map and MAM analysis.</li><li>Achieve superior sensitivity and reproducibility for acidic glycans and lipids by minimizing analyte/surface interactions that lead to sample loss.</li><li>Save time and improve productivity by reducing or eliminating passivation for robust oligonucleotide analyses.</li><li>Minimize the risk of undetected peaks by minimizing metal-sensitive analyte loss, improving recovery and the lowering limits of detection.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Accord_LC_MS_System_The_figure_A_shows_the_extracted_ion_chromatograms_XIC_for_GFYPSDIAVEWESNGQPENNYK_PENNYK_peptide_unmodified_and_deamidated_peptides_collected_for_a_NIS_Tm_Ab_digest_b14145461f.jpg" alt="Waters BioAccord LC-MS System: The figure (A) shows the extracted ion chromatograms (XIC) for GFYPSDIAVEWESNGQPENNYK (PENNYK) peptide unmodified and deamidated peptides collected for a NISTmAb digest. The chromatographic trace in solid line shows the XIC for PENNY peptides acquired on the BioAccord System. The inset shows all modification of PENNY peptide including succinimide modification. Figure (B) shows the total normalized peak area calculated for PENNY peptides using conventional and ACQUITY Premier UPLC system." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Accord_LC_MS_System_The_figure_A_shows_the_extracted_ion_chromatograms_XIC_for_GFYPSDIAVEWESNGQPENNYK_PENNYK_peptide_unmodified_and_deamidated_peptides_collected_for_a_NIS_Tm_Ab_digest_b14145461f.jpg 245w," sizes="100vw" width="1024" height="512"></p><h3><strong>Obtain quality data, faster than ever before</strong></h3><p>Quickly getting the quality data you need for your laboratory to stay productive is paramount but deploying traditional LC-MS systems is often rather slow. The integrated nature of the BioAccord LC-MS System means the system can be deployed easily and users can be productive faster through the delivery of system and applications training with appropriate biopharmaceutical methods, sample data, and system check standards for the common biopharmaceutical analysis workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Bio_Accord_LC_MS_System_Mass_accuracy_summary_for_48_injections_that_representing_six_antibodies_with_eight_replicate_injections_each_ed012d8ad7.jpg" alt="Waters BioAccord LC-MS System: Mass accuracy summary for 48 injections that representing six antibodies with eight replicate injections each. Mass accuracy summary for 48 injections that representing six antibodies with eight replicate injections each." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Bio_Accord_LC_MS_System_Mass_accuracy_summary_for_48_injections_that_representing_six_antibodies_with_eight_replicate_injections_each_ed012d8ad7.jpg 245w," sizes="100vw" width="855" height="206"></p><h4><strong>With the BioAccord LC-MS System, you can:</strong></h4><ul><li>Ensure optimal system performance with biomolecule-based installation testing.</li><li>Enable users to quickly get up to speed with outcome-based application training.</li><li>Achieve high quality data with existing personnel or respond quickly to staff and project turnover challenges with reduced training burdens.</li></ul><h3><strong>Meeting your regulatory requirements, together</strong></h3><p>Meeting corporate and regulatory expectations for compliance and data integrity is critical to your laboratory operations, and those needs don’t change because LC-MS technology is moving into roles within manufacturing and quality control (QC) organizations.</p><p>Your data quality and compliance are our priority, too. The BioAccord System is built on the compliant-ready waters_connect informatics platform and backed by the long history at Waters of delivering compliant informatics and professional qualification services to streamline deployment.</p><h4><strong>With Waters, you’ll get:</strong></h4><ul><li>A single informatics platform based on a secure relational database architecture</li><li>Full audit trail for acquisition, processing, and reporting of data</li><li>Administrator-configurable roles and permissions</li><li>Network deployment of systems for maximum data safety and controlled accessibility</li><li>Characterization (Q-Tof) and routine analysis (BioAccord) systems on a common network for efficient system, data, and information management</li><li>Professional qualification services</li></ul></div></div>

Waters SELECT SERIES MRT mass spectrometer

Accelerate your research and comprehensively identify analytes in your complex samples with the SELECT SERIES MRT, built with state-of-the-art Multi Reflecting Time-of-Flight (MRT) technology. 

<div><div id="title-089dfa8549" data-locator="title-with-text"><h3><strong>Redefine performance with all the benefits of time-of-flight technology</strong></h3></div></div><div><div id="text-77997605f8" data-locator="text"><p><strong>Accelerate your research and comprehensively identify analytes in your complex samples with the SELECT SERIES MRT, built with state-of-the-art Multi Reflecting Time-of-Flight (MRT) technology. The SELECT SERIES MRT provides a unique combination of ultra-high resolution and specificity independent of scan speed, resulting in unambiguous compound identification with even the most complex samples.</strong></p><p>Distinguish the fine isotope structure of small molecules, resolve nominally isobaric interferences, and baseline resolve the isotopic envelope of multiply-charged ions with high resolution MRT technology. Unlike other high-resolution mass analyzers, SELECT SERIES MRT requires no compromise to your experiments, since mass resolution is consistent over a broad mass range and scan speeds.</p><h3><strong>Overview</strong></h3><div data-locator="section-container-body"><div><div><div data-locator="layoutcontainer-"><div><div id="text-b57ec45d49" data-locator="text"><ul><li>Confidently identify sample components with routine ppb mass accuracy&nbsp;</li><li>Resolve nominally isobaric interferences with high mass resolution, at all scan speeds</li><li>Reveal isotopic fine structure for unambiguous elemental compositions</li><li>Achieve high data quality in both MS and MS/MS</li><li>Simplify data archiving and rapidly process data with reduced data files sizes</li><li>Seamlessly interchange between MALDI, DESI XS and Electrospray sources for superior accuracy for compound identification</li></ul></div></div><div><div id="text-590eba5dee" data-locator="text"><h3><strong>Recommended Use</strong></h3><p>For unambiguous compound identification of even the most complex samples.</p><div><div id="text-d3d9e01296" data-locator="text"><h3 data-locator="modal-title"><strong>High resolution mass spectrometry, without compromise</strong></h3><p>The SELECT SERIES MRT achieves ground-breaking Time-of-Flight (ToF) resolution with the innovative use of multiple reflections of the ion beam between two gridless electrostatic mirrors in the mass analyzer. This extends the ions’ flight&nbsp;path, spatially refocusing the ion packets through a series of periodic lenses after each reflection maintaining sensitivity.&nbsp;Resolution Enhancement Mode (REM) extends the flight path by deflecting ions back for a second pass of the MRT analyzer increasing the resolving power by &gt;50% enabling &gt;300,000 FWHM.</p><figure class="image"><img style="aspect-ratio:1272/897;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Schema_konstrukce_SELECT_SERIES_MRT_Q_Tof_systemu_a07ed0a724/Waters-Schema-konstrukce-SELECT-SERIES-MRT-QTof-systemu.jpg" alt="Waters SELECT SERIES MRT: instrument schematic." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Schema_konstrukce_SELECT_SERIES_MRT_Q_Tof_systemu_a07ed0a724/thumbnail_Waters-Schema-konstrukce-SELECT-SERIES-MRT-QTof-systemu.jpg 221w," sizes="100vw" width="1272" height="897"></figure><p>The superior performance of the SELECT SERIES MRT results in high precision and routine part-per-billion mass accuracy independent of acquisition speed, providing unsurpassed quality of mass spectrometry data and information. The instrument is operated via the SELECT SERIES&nbsp;<a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=513164" data-di-id="di-id-e7513c98-b105e917">MassLynx</a>&nbsp;acquisition system with simple and intuitive experimental setup, acquisition, and processing of mass spectrometry data. It records data in the widely deployed Waters .raw file format, which you can easily access via the MassLynx API, export as (i)mzML, and other interchangeable data formats, ready to use in your data analysis strategy.</p><figure class="image"><img style="aspect-ratio:1024/682;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_MRT_REM_mode_fine_isotope_structure_for_caffeine_1443227594.jpg" alt="Waters SELECT SERIES MRT: REM mode fine isotope structure for caffeine." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_MRT_REM_mode_fine_isotope_structure_for_caffeine_1443227594.jpg 234w," sizes="100vw" width="1024" height="682"></figure><div><div id="text-78cca88bb7" data-locator="text"><h3 data-locator="modal-title"><strong>Redefining confidence for LC-MS</strong></h3><p>The SELECT SERIES MRT provides a new level of confidence for LC/MS experiments by routinely obtaining ppb mass accuracy, revealing fine isotope structure, all with the exceptional usability of a state-of-the-art Quadrupole Time-of-Flight (QToF). The spectacular performance enables search tolerances to be minimized, providing higher confidence in elemental composition and molecular formula assignments for known and unknown targets. Moreover, the ability to routinely access such high specificity from accurate mass for both precursor and product ions is truly transformative.</p><figure class="image"><img style="aspect-ratio:1024/570;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_MRT_Resolving_deamidations_for_confident_quantification_356c8f4783.jpg" alt="Waters SELECT SERIES MRT: Resolving deamidations for confident quantification. A) The deamidated forms of peptide HC:T23 elute in the tail of the native peptide peak. Without the ultra-high resolution of the SELECT SERIES MRT as shown in (B) identification and quantification of the deamidations." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_MRT_Resolving_deamidations_for_confident_quantification_356c8f4783.jpg 245w," sizes="100vw" width="1024" height="570"></figure><div><div id="text-1092d8da82" data-locator="text"><h3 data-locator="modal-title"><strong>Redefining clarity in mass spectrometry imaging</strong></h3><p>The SELECT SERIES MRT reduces analysis times by providing crystal clear, high-resolution images, acquired at fast imaging speeds without compromising mass spectral resolution or accuracy. This enables accelerated mass spectrometry imaging experiments while still resolving analytes from interferences, revealing fine structural information. The cutting-edge technology and intuitive software combine to revolutionize mass spectrometry imaging for a wide range of applications; where the ability to map compounds that differ by only a few mDa is changing the pace of research. This includes:</p><ul><li>Tissue analysis in biomedical research</li><li>Forensic research, including fingerprint analysis</li><li>Surface analysis, ranging from surface contamination analysis for semiconductor production, to analysis of tape stripped skin samples in the cosmetics industry</li></ul><div><div id="text-7f1b1e82f7" data-locator="text"><h3 data-locator="modal-title"><strong>Maximize MS imaging capabilities with DESI and MALDI</strong></h3><p>Maximize your mass spectrometry imaging capabilities and analytical flexibility with both DESI and MALDI on the SELECT SERIES MRT. Pair the SELECT SERIES MRT with <a target="_blank" rel="noopener noreferrer" href="https://www.waters.com/waters/nav.htm?cid=135047466" data-di-id="di-id-5d203d9f-b105e917">DESI XS</a>&nbsp;for high quality data and clear visualization of molecular spatial distributions. The speed and versatility of DESI imaging makes it best suited to the analysis of lipids and other small molecules under ambient, direct analysis conditions, without the need for additional matrices.</p><p>Use the high-performance MALDI source to improve imaging performance and ease-of-use, so you can rapidly image large tissue sections at high spatial resolution with improved selectivity and sensitivity. MALDI’s design uses a tightly focused laser beam profile, increasing signal-to-noise at high spatial resolutions and providing lateral resolution of 15 µm without oversampling, with data acquisition rates &gt;10 pixels per second, so scientists can acquire high intensity images quickly and efficiently. With MALDI and SELECT SERIES MRT, you can:</p><ul><li>Enable imaging of large sections with a full-sized Micro Titre Plate (MTP) format MALDI target plate</li><li>Access high spatial resolution through increased signal to noise (S/N), with a focused laser beam profile</li><li>Simplify usability with an automated isolation valve and laser focus control</li><li>Maximize productivity and uptime with easy cleaning and maintenance</li></ul><figure class="image"><img style="aspect-ratio:1349/1093;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_SELECT_SERIES_MRT_2_8ead9924a6/Waters-SELECT-SERIES-MRT-2.jpg" alt="Waters SELECT SERIES MRT." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_SELECT_SERIES_MRT_2_8ead9924a6/thumbnail_Waters-SELECT-SERIES-MRT-2.jpg 193w," sizes="100vw" width="1349" height="1093"></figure></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div></div>

Products focused on LC/TOF - page 2

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