ISC 2026 - Day 2

<h4>What if you could compress 179 days of grueling laboratory workflow into just 14 hours?</h4>In this episode of ‪ChromatographyTalk‬, ‪Organomation‬ General Manager David Oliva sits down with analytical chemist Laura Penabad to discuss her groundbreaking new research in biocatalysis and high-throughput screening. Evolving engineered enzymes typically requires checking thousands of variants—a massive bottleneck when traditional Liquid Chromatography-Mass Spectrometry (LC-MS) runs take minutes per sample. Laura reveals how her team built a modular screening platform that shatters this limitation, running samples 128 times faster than standard methods without sacrificing analytical accuracy.Laura breaks down the "orchestra" of technologies making this possible, including coupling nanoliter droplet microfluidics with Cyclic Ion Mobility-Mass Spectrometry (cIM-MS) to separate complex chemical isomers in milliseconds. She also shares the chaotic but triumphant behind-the-scenes stories of her PhD journey—from presenting a "comically bad" early poster at ASMS to recruiting an undergraduate mechanical engineer to build custom automation software from scratch.If you are an early-career researcher, a mass spec enthusiast, or curious about the future of synthetic biology and machine learning in chemistry, this episode is packed with invaluable intuition and advice.<h3>Video Transcription</h3><h4>From Months to Hours: Droplet Ion Mobility–MS for Biocatalysis Screening</h4>High-throughput screening is central to directed enzyme evolution, but conventional analytical workflows can create a difficult compromise: extremely fast screens often provide limited structural information, while techniques such as LC-MS offer much richer chemical insight at the cost of throughput. In a recent interview, analytical chemist Laura described how her work with droplet-based cyclic ion mobility mass spectrometry is helping bridge that gap—providing rapid analysis while retaining the ability to distinguish structurally similar products.Her research grew from an initially modest proof of concept into a screening platform capable of analyzing a 96-well plate in roughly ten minutes. The resulting workflow was approximately 128 times faster than a conventional three-minute LC-MS analysis, opening new possibilities for directed evolution campaigns in which thousands of enzyme variants need to be evaluated.<h4>Directed evolution needs better analytical screening</h4>Biocatalysis takes advantage of enzymes as highly selective catalysts capable of performing reactions under relatively mild conditions. Directed evolution extends those capabilities by deliberately modifying enzyme sequences, expressing large numbers of variants, screening their activity, and using the best-performing mutants as starting points for subsequent rounds of evolution.The process can be repeated multiple times, progressively steering an enzyme toward improved activity, altered substrate specificity, or even chemistry that does not occur naturally.But the effectiveness of directed evolution depends heavily on the screening step. The more variants that can be analyzed—and the more useful chemical information that can be collected for each one—the more effectively researchers can explore the enormous sequence space available to an enzyme.For Laura, this created a clear analytical challenge: develop a screening platform that was substantially faster than conventional chromatographic analysis without sacrificing the structural discrimination needed for chemically similar products.<h4>From an imperfect first experiment to a working platform</h4>The project did not begin with a polished high-throughput workflow.Laura recalled looking back at her 2023 ASMS poster, where the early system was still working with standards rather than true isomeric samples. Droplet introduction was slow—around six seconds per droplet—and the signals were far from the ideal reproducible, square, flat-topped profiles expected from replicate droplets.Nevertheless, simply producing a droplet signal represented an important first milestone.The decisive change came when the project stopped being treated merely as a demonstration that droplet ion mobility–MS could work and instead became a deliberate effort to build a robust screening platform.Laura approached the problem in modules. First came reliable droplet introduction. Then the ion mobility separation method. Quantification became another independent component. Matrix effects were introduced later, gradually making the system resemble the conditions expected with real screening samples.Each layer was tested before another was added.This stepwise strategy became one of the central principles of the project: rather than attempting to optimize the entire system simultaneously, individual components were developed, challenged, and then assembled into an increasingly realistic workflow.<h4>Finding the middle ground between speed and structural information</h4>One of the biggest analytical problems in high-throughput screening is distinguishing products with the same nominal or exact mass.Constitutional isomers may contain the same atoms and therefore generate the same mass-to-charge ratio, while differing only in how functional groups are arranged within the molecular structure. Very rapid optical or mass-based screens may therefore struggle to distinguish them.Traditional LC-MS can solve many of these problems through chromatographic separation, but a several-minute analysis rapidly becomes impractical when thousands of enzyme variants must be screened.Ion mobility offered Laura’s team another option.Rather than separating compounds on a chromatographic timescale, ion mobility can differentiate ions according to their gas-phase behavior on a much shorter timescale. In the workflow described in the interview, this created a useful compromise: not the kilohertz throughput possible with some simple screening technologies, but dramatically faster structural differentiation than conventional LC-MS.The goal was therefore not simply to achieve the fastest possible screen, but to find a middle-throughput analytical regime with enough resolving power to answer chemically meaningful questions.<h4>A 96-well plate in about ten minutes</h4>That strategy eventually produced one of the most striking results of the project.The droplet cyclic ion mobility–MS platform processed a 96-well plate in roughly ten minutes, while maintaining quantitative results comparable with the conventional LC-MS workflow used for comparison. According to the interview, this represented an approximately 128-fold increase in analysis speed relative to a three-minute LC-MS run.Laura described the successful plate experiment less as a surprise than as confirmation that the modular development strategy had worked.Before reaching that point, the workflow had already been challenged through a sequence of increasingly demanding experiments: droplet formation, ion mobility separation, calibration, matrix-containing calibration, and cross-testing analytes at different concentrations. By the time the complete plate was analyzed, each individual part of the analytical system had already been tested under progressively more realistic conditions.<h4>What does that speed mean for directed evolution?</h4>The impact becomes even clearer when the workflow is considered at the scale of an entire directed-evolution campaign.Laura’s manuscript estimated that a five-round campaign involving 5,000 variants would require approximately 179 days using the conventional LC-MS workflow but only about 14 hours using the new platform.Compressing months of analytical work into less than a day has consequences beyond laboratory productivity.It allows researchers to screen more enzyme variants and therefore sample a larger portion of sequence space. But the resulting datasets may also become increasingly valuable for computational approaches.Rather than retaining information only about the best-performing enzyme variant, researchers can preserve data describing variants that performed poorly as well as those that succeeded. When genotype and phenotype information remain linked through the well-plate workflow, these larger datasets can potentially provide richer input for machine-learning models designed to guide subsequent rounds of enzyme evolution.Faster analysis therefore does more than accelerate an existing experiment—it can change the scale and design of the experiment itself.<h4>Balancing ion mobility resolution and signal</h4>Increasing cyclic ion mobility separation time can improve resolution, but it also introduces an important trade-off: additional passes can reduce signal.Laura optimized this balance experimentally by continuously infusing mixtures containing both isomers and progressively adjusting the ion mobility conditions. After tuning parameters such as wave height and velocity, increasing the separation to three passes provided baseline resolution at the high analyte concentrations used during method development.<blockquote>Concentration, however, also mattered.</blockquote>At higher ion populations, space-charge effects could broaden peaks and reduce the baseline separation. This meant that the optimal number of passes could not be selected independently of the concentration range required by the final assay.Her broader advice for optimization is therefore application-driven: decide first what the analytical method actually needs to accomplish.More speed will almost always require sacrificing something—resolution, signal intensity, quantitative precision, or another performance parameter. The key is to identify which compromise the application can tolerate and then optimize the system around that requirement.<h4>Solving droplet carryover with a practical compromise</h4>Droplet-based workflows present their own engineering challenges, including carryover between neighboring samples.In Laura’s system, analytes showed little tendency to partition into the fluorinated oil separating aqueous droplets within the Teflon tubing. The more troublesome carryover occurred closer to electrospray ionization, where a small amount of sample could remain associated with the interface and appear when the next droplet arrived.Rather than adding excessive engineering complexity to an already elaborate platform, the researchers adopted a pragmatic solution: a sacrificial wash droplet between analytical samples.It is an example of a recurring theme throughout the project. The objective was not to construct the theoretically most sophisticated droplet system, but to create a screening workflow that remained sufficiently robust, reproducible, and practical for large numbers of samples.<h4>High-throughput analysis also requires high-throughput data processing</h4>Accelerating the mass spectrometry experiment created another bottleneck: the data.The instrument produced a continuous intensity-versus-time trace rather than a neatly organized well-plate dataset. With small experiments, Laura could manually identify individual droplets and process the results in spreadsheets. That approach quickly became unrealistic as sample numbers increased.The solution emerged through collaboration with an undergraduate mentee, Aiden, who helped develop a dedicated software tool for processing the continuous traces.Laura defined how the software should behave and how the analytical information should be organized, while Aiden developed the computational implementation. The workflow itself was subsequently adjusted to make automated data processing more reliable—for example, by introducing marker columns that allowed the software to recognize the end of each plate row.The resulting tool can accept the acquired traces together with information about plate dimensions and replicates and automatically return parameters including peak identity, duration, and the corresponding well position. A later version also generated an automatically populated heat map.The experience illustrates how improving analytical throughput often requires simultaneous development of instrumentation, experimental design, and informatics. Making the measurement faster is useful only if the resulting data can be processed at a comparable speed.<h4>Mentoring became part of the scientific process</h4>The software project also reflects another theme Laura emphasized throughout the interview: the value of mentoring.Several younger researchers contributed directly to the development of the final platform. Laura described mentees not simply as additional hands in the laboratory but as scientists capable of proposing their own hypotheses and experimental ideas.Working with undergraduate researchers also influenced the way she organized experiments.Because their available laboratory time was limited, each task needed to have a clear purpose. That encouraged a more disciplined approach to experimental planning: instead of performing experiments simply because they could be done, the team had to ask which experiment would most efficiently answer the next important question.In that sense, mentoring became another mechanism for improving the research itself.<h4>The next step: continuous, automated screening</h4>Despite the dramatic increase in analytical speed, the current workflow still includes manual operations such as loading tubing and connecting the sample stream to the mass spectrometer.The next engineering challenge is therefore automation.Laura described ongoing work using the Venturi effect to pull samples directly from wells into the droplet system. Building on previous work in the laboratory with 96-well plates, she has already tested this approach with as many as 2,000 samples.The long-term direction is clear: continuous and robust droplet generation, automated handling of thousands of samples, rapid ion mobility separation, mass spectrometric detection, and automated reconstruction of the resulting data into plate-based outputs.Combining those elements could move the system closer to the type of large-scale screening required for increasingly ambitious biocatalysis campaigns.<h4>Learning mass spectrometry one problem at a time</h4>Laura also reflected on the broader experience of developing the platform during her PhD.Ion mobility and mass spectrometry initially appeared intimidating, particularly because they combine concepts from analytical chemistry, physical chemistry, separations, instrumentation, and electrical engineering. What eventually changed was not simply learning more facts, but developing the ability to move repeatedly between theory and experiment.An unexpected result sends the researcher back to the underlying theory. A better understanding suggests a modified experiment. That experiment creates another observation, gradually building intuition about how the system behaves.Her advice to early-career scientists is therefore to accept that this process takes time.Advanced analytical techniques may initially appear overwhelming, but understanding develops incrementally through repeated interaction between theory, experimentation, failure, and refinement. Equally important, she emphasized the role of the supportive ion mobility–mass spectrometry community in making a technically demanding field more approachable for young researchers.From the first inconsistent droplets to a platform capable of processing thousands of samples, that iterative mindset ultimately shaped the entire project.<blockquote>This text has been automatically transcribed from a video presentation using AI technology. It may contain inaccuracies and is not guaranteed to be 100% correct.</blockquote><blockquote><h4>Concentrating on Chromatography Podcast</h4>Dive into the frontiers of chromatography, mass spectrometry, and sample preparation with host David Oliva. Each episode features candid conversations with leading researchers, industry innovators, and passionate scientists who are shaping the future of analytical chemistry. From decoding PFAS detection challenges to exploring the latest in AI-assisted liquid chromatography, this show uncovers practical workflows, sustainability breakthroughs, and the real-world impact of separation science. Whether you’re a chromatographer, lab professional, or researcher you'll discover inspiring content!You can find Concentrating on Chromatography Podcast in podcast apps:<ul><li><a target="_blank" rel="noopener noreferrer" href="https://open.spotify.com/show/1wZg67rCjoGju4yhqxI3tL">Spotify</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podcasts.apple.com/tz/podcast/concentrating-on-chromatography/id1788429185">Apple podcast</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://podscan.fm/podcasts/concentrating-on-chromatography">podscan</a></li></ul>and on <a target="_blank" rel="noopener noreferrer" href="https://www.youtube.com/@ChromatographyTalk/videos">YouTube channel</a></blockquote>

Droplet Cyclic Ion Mobility Mass Spec: Compressing Months of Enzyme Screening Into Just 14 Hours

ISC 2026 - Day 3

Article

Events

Video

Scientists

Interview

The International Symposium on Chromatography (ISC) represents the oldest conference series focussing on separation science. ISC symposia have been organised since 1956 in each even year. ISC is internationally recognised as one of the premier meeting series for discussion of all modes of chromatography and separation science with a broad coverage of techniques and applications.

International Symposium on Chromatography - (ISC)

Organomation, founded in 1959, designs and manufactures high-quality nitrogen evaporators and extraction systems. Known for innovation and durability, their lab instruments are used globally for efficient sample preparation with strong customer support.

Organomation

Thermo Scientific Acclaim C30 HPLC Columns

For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. 

For separations of hydrophobic, structurally related isomers, Thermo Scientific™ Acclaim™ C30 LC reversed-phase columns provide superior results. The covalent modification of C30 alkyl silane on ultrapure, spherical, porous silica gel gives these columns high shape selectivity. They are fully compatible with various aqueous buffers, useful for a broad application range of analytes, and offer more flexibility in method development.<h5>High Shape Selectivity for the Separation of Structurally Related Isomers</h5><ul><li>Unique selectivity complementary to other reversed-phase columns</li><li>High efficiency and rugged packing</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 3 and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 200Å</li></ul><h4>Specifications</h4><ul><li>Max. Pressure: 8000 psi (550 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary Phase: C30</li><li>Surface Area: 200 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L62</li><li>Carbon load: 13%</li><li>Phase: Reversed phase</li><li>Pore Size: 200 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim HILIC-10 HPLC Columns

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. 

Separate highly hydrophilic molecules by hydrophilic interaction liquid chromatography using Thermo Scientific™ Acclaim™ HILIC-10 LC Columns. These columns retain and separate highly polar molecules that are unretained by reversed-phase chromatography. Their high-purity, spherical, porous silica base is covalently modified with a proprietary hydrophilic layer. This chemistry enables the use of 5–20% aqueous mobile phase while maintaining affinity for polar analytes. Sample throughput is increased without the need to evaporate and reconstitute samples dissolved in nonaqueous solvents.<h5>Rugged and Reproducible Separations of Highly Polar Compounds:</h5><ul><li>Unique selectivity, complementary to reversed-phase columns</li><li>Enhanced sensitivity for MS detection</li><li>Hydrolytically stable</li><li>Rugged column packing</li><li>Broad application range, including separation of hydrophilic drugs and drug metabolites</li></ul>Acclaim HILIC-10 LC columns are available in 3μm particle size and a variety of column formats: 4.6×150mm, for high-throughput routine analysis; 3.0×150mm, for high-throughput analysis with reduced solvent consumption; and 2.1×150mm, for LC-MS analysis.<h4>Specifications</h4><ul><li>Column type: HILIC</li><li>Max. Pressure: 6000 psi (415 bar)</li><li>Packing Material: Silica</li><li>Surface Area: 300 m²/g</li><li>Temperature: 70°C</li><li>USP Type: N/A</li><li>Carbon load: 8%</li><li>Phase: HILIC</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Polar Advantage HPLC Columns

Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns.

Resolve a wide variety of polar and nonpolar analytes with Thermo Scientific™ Acclaim™ PolarAdvantage (PA) columns. These sulfonamide-embedded, reversed-phase columns provide unique selectivity and excellent peak shape for acidic, basic, and neutral analytes.&nbsp;In addition, the mildly hydrophilic surface, which remains in contact with aqueous-only mobile phases, negates the problems of dewetting common with conventional C18 reversed-phase columns. Use Acclaim PA columns for efficient separations of nucleic acid and bases or pharmaceutical compounds using highly aqueous mobile phases.<h5>Unique Selectivity for the Separation of Polar and nonpolar Analytes</h5><ul><li>Low silanol activity for excellent peak shape with basic compounds</li><li>High selectivity for hydrophobic aromatic molecules</li><li>Compatible with 100% aqueous mobile phase</li><li>Extremely low bleed, fully compatible with MS</li><li>Stable between pH 2 and 8</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li><li><h4>Specifications</h4></li><li>Endcapped: Yes</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Polar Embedded</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L60</li><li>Carbon load: 17%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 2 to 8</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Polar Advantage II HPLC Columns

Resolve polar and nonpolar compounds in a single run with Thermo Scientific Acclaim PolarAdvantage II (PA2) reversed-phase columns.

Resolve polar and nonpolar compounds in a single run with Thermo Scientific™ Acclaim™ PolarAdvantage II (PA2) reversed-phase columns. These high-efficiency, silica-based columns have a polar-embedded stationary phase that operates over a wider range of chromatographic conditions than possible with conventional reversed-phase stationary phases. Its unique chemistry provides enhanced hydrolytic stability from pH 1.5 to 10 with 100% aqueous mobile phases. The column exhibits selectivity that is complementary to conventional C18 columns and excellent peak shapes for both basic and acidic compounds.<h5>Complementary Selectivity and Enhanced Hydrolytic Stability</h5><ul><li>Compatible with 100% aqueous mobile phases</li><li>High reversed-phase capacity</li><li>Exceptional hydrolytic stability from pH 1.5 to 10</li><li>Extremely low bleed, fully compatible with mass spectrometry (MS)</li><li>Available in 2.2, 3, and 5μm particle sizes and 4.6, 3.0, and 2.1mm diameters, with an average pore diameter of 120Å</li><li>Also available in 2.2μm RSLC format for reliable, fast separations</li><li><h4>Specifications</h4></li><li>Endcapped: Yes</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Polar Embedded</li><li>Surface Area: 300 m²/g</li><li>Temperature: 60°C</li><li>USP Type: L60</li><li>Carbon load: 17%</li><li>Phase: Reversed phase</li><li>Pore Size: 120 Å</li><li>pH: 1.5 to 10</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Trinity P1 HPLC Columns

Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific Acclaim Trinity P1 LC Columns.

Simultaneously separate pharmaceutical drug substances and their counterions with high-efficiency Thermo Scientific™ Acclaim™ Trinity P1 LC Columns. A unique trimodal surface chemistry provides concurrent reversed-phase, cation-exchange and anion-exchange functionalities for maximum flexibility in method development. Easily optimize selectivity by adjusting mobile phase buffer concentration, pH and solvent content—either jointly or independently—to accelerate chromatographic separations and increase productivity.The Acclaim Trinity P1 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical 3μm silica particles, coated with charged nanopolymer beads. The unique surface chemistry includes an inner-pore area modified with an organic layer that provides both reversed-phase and anion-exchange properties. The outer-pore surface, conversely, is modified with cation-exchange functionality for unparalleled chromatographic performance.<h5>Superior Performance for Pharmaceutical Applications</h5><ul><li>Ideal selectivity for simultaneous separation of Active Pharmaceutical Ingredients (API) and counterions (basic, neutral and acidic analytes)</li><li>Effective retention of hydrophilic ionic and ionizable analytes without ion-pairing reagents</li><li>Distinctive spatial separation of the anion-exchange and cation-exchange regions</li><li>Flexible method development with each retention mechanism controlled independently</li><li>Low column bleed; compatible with mass spectrometry (MS), charged aerosol detection (CAD) and evaporative light scattering detectors (ELSD)</li><li>Available in 2.1 and 3.0mm diameters and 50, 100 and 150mm lengths</li></ul>Complement the Acclaim Trinity P1 column with the Acclaim Trinity P2 column to provide a total solution for pharmaceutical counterion analysis by HPLC. Use the Acclaim Trinity P2 column to separate counterions, including monovalent and divalent cations or anions.<h4>Specifications</h4><ul><li>For use with: ELSD, UV Detector</li><li>Max. Pressure: 4500 psi (310 bar)</li><li>Packing Material: Spherical, Nanopolymer Hybrid Silica</li><li>Stationary phase: Reversed Phase/Anion Exchange/Cation Exchange</li><li>Surface Area: 100 m²/g</li><li>Temperature: 45°C</li><li>Phase: Reversed Phase and Ion Exchange</li><li>Pore Size: 300 Å</li><li>pH: 2.5 to 7</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Trinity P2 HPLC Columns

Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific Acclaim Trinity P2 LC Columns.

Separate pharmaceutical counterions, including monovalent and divalent cations or anions, with Thermo Scientific™ Acclaim™ Trinity P2 LC Columns. The innovative, mixed-mode column technology provides hydrophilic interaction, HILIC, anion-exchange, and cation-exchange functionalities for flexible method development. Use the Acclaim Trinity P2 LC column to complement the Acclaim Trinity P1 LC column, which simultaneously separates pharmaceutical drug substances and their counterions, to provide a total solution for pharmaceutical counterion analysis by HPLC.The Acclaim Trinity P2 stationary phase, based on Nanopolymer Silica Hybrid (NSH) technology, consists of high-purity porous, spherical silica particles, coated with charged nanopolymer particles. The unique surface chemistry includes an inner-pore area of the silica bead modified with a covalently bonded organic layer that provides cation-exchange retention. The outer-pore surface is modified with anion-exchange nano-polymer beads. This chemistry ensures spatial separation of the anion-exchange and cation-exchange regions.<h5>High Selectivity for Counterion Analysis</h5><ul><li>Low column bleed; compatible with mass spectrometry (MS) and charged aerosol detection (CAD)</li><li>Hydrolytically stable</li><li>High efficiency</li><li>Available in 3.0μm particle size and 2.1 and 3.0mm diameters, in 50 and 100mm lengths</li></ul><h4>Specifications</h4><ul><li>Max. Pressure: 6000 psi (415 bar)</li><li>Packing Material: Spherical, Fully Porous Silica</li><li>Stationary phase: Spherical, Nanopolymer Hybrid Silica</li><li>Surface Area: 100 m²/g</li><li>Temperature: 45°C</li><li>Phase: HILIC and Ion Exchange</li><li>Pore Size: 300 Å</li><li>pH: 2.5 to 7</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Mixed Mode WAX-1 HPLC Columns

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific Acclaim Mixed-Mode WAX-1 LC columns. 

When you need powerful selectivity control for the separation of anionic molecules, use Thermo Scientific™ Acclaim™ Mixed-Mode WAX-1 LC columns. The silica-based stationary phase features a hydrophobic alkyl chain with an ionizable terminus that provides both hydrophobic reversed-phase and weak anion-exchange properties. This unique chemistry produces a multimode separation mechanism for simultaneous separation of acidic, basic, and neutral molecules. The selectivity is fully adjustable through changes to ionic strength, pH, or organic solvent content.<h5>Ideal Selectivity for Separating Anionic Molecules</h5><ul><li>Multi-mode retention mechanism: reversed-phase, weak anion-exchange, and HILIC modes</li><li>Differentiation of molecules with similar hydrophobicities, such as hydrophilic organic acids</li><li>Selectivity orthogonal to reversed-phase columns</li><li>Excellent column efficiency and peak symmetry</li><li>Stable between pH 2.5 and 7.5</li><li>Available in 3 and 5μm particle sizes with an average pore diameter of 120Å</li></ul><h4>Specifications</h4><ul><li>Column type: Mixed mode</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Hydrophobic Alkyl Chain with an Ionizable Terminus</li><li>USP Type: L78</li><li>Surface Area: 300 m²/g</li><li>Temperature: 50°C</li><li>Phase: Reversed Phase, Ion Exchange, HILIC</li><li>Pore Size: 120 Å</li><li>pH: 2.5 to 7.5</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Mixed Mode WCX-1 HPLC Columns

Separate cationic molecules with powerful adjustable selectivity control using Thermo Scientific Acclaim Mixed-Mode WCX-1 LC columns.

Separate cationic molecules with powerful adjustable selectivity control using Thermo Scientific™ Acclaim™ Mixed-Mode WCX-1 LC columns. The silica-based stationary phase features a hydrophobic alkyl chain with a carboxyl terminus that provides both hydrophobic reversed-phase and weak cation-exchange properties. This multimode separation mechanism delivers simultaneous separation of basic and neutral compounds. By independently or simultaneously tuning the mobile phase ionic strength, pH, and organic modifier, you can control the selectivity of ionizable and neutral compounds.<h5>Retains and Separates Highly Hydrophilic to Highly Hydrophobic Basic Molecules</h5><ul><li>Multimode retention mechanism: reversed-phase, weak cation-exchange, anion-exclusion, and HILIC modes</li><li>Selectivity complementary to C18 reversed-phase columns</li><li>Efficient primary column for a variety of basic analytes; also an ideal column for secondary methods development</li><li>Versatile in applications that require different selectivities</li><li>Symmetrical peak shapes and excellent efficiency</li><li>Stable between pH 2.5 and 7.5</li><li>Available in 3 and 5μm particle sizes with an average pore diameter of 120Å</li></ul><h4>Specifications</h4><ul><li>Column type: Mixed mode</li><li>For use with: RPLC, Cation Exchange, Ion-Exclusion, HILIC</li><li>Max. Pressure: 6000 psi (415 bar)</li><li>Packing Material: Silica</li><li>Stationary phase: Hydrophobic Alkyl Chain with a Carboxyl Terminus</li><li>USP Type: L85</li><li>Surface Area: 300 m²/g</li><li>Temperature: 50°C</li><li>Phase: Reversed Phase, Ion Exchange, Ion Exclusion, HILIC</li><li>Pore Size: 120 Å</li><li>pH: 2.5 to 7.5</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim Mixed Mode HILIC-1 HPLC Columns

Get all the advantages of reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific Acclaim Mixed-Mode HILIC-1 column.

Get all the advantages of reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) in one column with the Thermo Scientific™ Acclaim™ Mixed-Mode HILIC-1 column. The high-efficiency, silica-based, mixed-mode stationary phase consists of a hydrophobic alkyl chain with a diol group at the terminus. The hydrophobic moiety supplies reversed-phase retention and the terminal diol group facilitates hydrophilic interactions. This unique combination provides adjustable selectivity with unsurpassed resolution and peak symmetry for a variety of both polar and nonpolar molecules.<h5>Separates Both Polar and Nonpolar Molecules with Selectivity Complementary to RP Columns</h5><ul><li>Operates in both RP and HILIC modes</li><li>Retains highly polar molecules</li><li>Covers broader application range than conventional diol-based columns, including nonionic ethoxylated surfactants, drug metabolites, lipids, and polyethylene glycols (PEGs)</li><li>Stable between pH 2.5 and 7.5</li><li>Available in 3 and 5μm particle sizes with an average pore diameter of 120Å</li></ul><h4>Specifications</h4><ul><li>Column type: Mixed mode</li><li>For use with: HILIC</li><li>Max. Pressure: 5800 psi (400 bar)</li><li>Packing Material: Spherical, Fully Porous, Ultrapure Silica</li><li>Stationary phase: Hydrophobic Alkyl Chain with a Diol Group</li><li>USP Type: N/A</li><li>Surface Area: 300 m²/g</li><li>Temperature: 50°C</li><li>Phase: Reversed Phase, HILIC</li><li>Pore Size: 120 Å</li><li>pH: 2.5 to 7.5</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Thermo Scientific Acclaim SEC-1000 HPLC Columns

Separate water-soluble polymers and oligomers with Thermo Scientific™ Acclaim™ SEC-1000 Size Exclusion HPLC Columns. 

Separate water-soluble polymers and oligomers with Thermo Scientific™ Acclaim™ SEC-1000 Size Exclusion HPLC Columns. These resin-based, high-performance, size-exclusion chromatography columns cover molecular weight range: 7μm, 1000Å. The combination of a proprietary mono-dispersed multi-pore hydrophilic resin and small particle size provides high-resolution and good linearity of the calibration curve with no inflection points for accurate, reliable analysis of a variety of water-soluble polymers.The Acclaim SEC-1000 LC column is calibrated from 1,000 to 1,000,000 Daltons and can characterize high molecular weight polymers.<h5>High-Performance Analysis of Water-Soluble Polymers</h5><ul><li>High-resolution analysis at reduced solvent consumption in available 4.6 x 300mm dimension</li><li>Stable surface bonding leads to low column bleed and enhances compatibility with UV, RI, MS, ELSD and Thermo Scientific Dionex™ Corona™ Charged Aerosol Detectors</li></ul><h4>Specifications</h4><ul><li>Column type: Size exclusion</li><li>Endcapped: yes</li><li>Max. Pressure: 600 psi (40 bar)</li><li>Packing Material: Spherical, Fully Porous Polymer</li><li>Temperature: 60°C</li><li>Phase: Size exclusion</li><li>Pore Size: 1000 Å</li><li>pH: 2 to 12</li></ul><h4>About the Acclaim Family of HPLC Columns</h4>Achieve the high standards set by modern HPLC and LC/MS methods with the Acclaim family of reversed-phase columns. These silica-based columns with advanced column bonding and endcapping technologies provide complementary selectivity, high column efficiencies, and ultra-high resolution for a wide variety of applications.<ul><li>Broad spectrum of stationary phases, including conventional C8 and C18 phases, polar embedded phases, and specialty phases including wide-pore, are available to meet your specific application needs</li><li>Performance tested to ensure high quality and reliability for pharmaceutical, biopharmaceutical, environmental, food, and other industrial chromatographic separations</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg" alt="Thermo Fisher Scientific: Broad range of HPLC and UHPLC columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_Acclaim_column_Broad_range_of_HPLC_and_UHPLC_columns_2ec9c98a86.jpg 191w," sizes="100vw" width="1172" height="958">

Products from category HPLC columns - page 9

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