<p><strong>Quantifiable consistency. By tracking retention time consistency and peak symmetry across years of production, column performance becomes directly comparable.</strong></p><p>In chromatography, inconsistent separations are inconvenient and costly.</p><p>Every unexpected shift in retention time or change in peak shape can trigger a cascade of troubleshooting, reruns, and uncertainty that slows laboratory productivity.</p><p>On the other hand, when columns perform consistently, laboratories recover valuable time, reduce uncertainty, and maintain confidence in their data. True batch-to-batch consistency means fewer failed validations, fewer investigations, easier audits, and smoother collaboration across global sites.</p><p>In regulated industries, data integrity underpins every decision, from early development to final release testing, so consistent column performance is a necessity.</p><h3><strong>Key takeaways</strong></h3><ul><li>Batch-to-batch (U)HPLC column consistency directly influences long-term method performance by stabilizing retention times, preserving peak shape, and supporting reliable validation of pharmaceutical methods.</li><li>Multi-year performance data across thousands of columns shows us how controlled column manufacturing reduces chromatographic variability, supporting reproducible results across column formats, batches, and laboratories.</li><li>Stable (U)HPLC columns minimize revalidation, troubleshooting, and method transfer challenges in regulated workflows, improving analytical efficiency across the product lifecycle.</li></ul><h3><strong>Defining and measuring batch-to-batch (U)HPLC column consistency</strong></h3><p>A (U)HPLC column is packed with <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/chromatography-consumables/consumable-types.html"><strong>solid-phase particles</strong></a><strong> </strong>that <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-system-components/how-hplc-columns-work.html"><strong>separate compounds by chemical structure</strong></a>.</p><p>If medium composition differs even slightly between manufacturing batches, retention times and peak shapes can change. These differences might seem minor, but in pharmaceutical analysis they can trigger failed validations, unnecessary troubleshooting, and inconsistent data across labs.</p><p>A recent evaluation of<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/TG_20762_LC_Hypersil_GOLD_TG_20762_EN_ebcf64d4d6/TG-20762-LC-Hypersil-GOLD-TG20762-EN.pdf"><strong> Thermo Scientific Hypersil GOLD columns</strong></a> examined whether<a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/lcms/products?category=48&amp;manufacturerItems=3&amp;search=gold"><strong> these columns </strong></a>perform consistently across years of production. The answer: our results found sustained performance over time.</p><p>Across roughly <strong>17,500 columns tested over three years</strong>, researchers focused on two major indicators of a manufacturer’s column performance:</p><ul><li>Relative retention time (RRT), which is essentially how consistently compounds elute off the column.</li><li>Peak asymmetry, a reflection of column packing quality and how well peaks “behave.”</li></ul><p>In a recent technical note:<strong> </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004127_ccs_hypersil_gold_reversed_phrase_reproducibility_tn004127_na_en_d6375a67fb.pdf"><strong>Evaluation of batch-to-batch consistency of reversed-phase HPLC columns for long-term method validation</strong></a>, we evaluated three column configurations: a 250 mm × 4.6 mm, 5 μm column; a 150 mm × 4.6 mm, 5 μm column; and 50 mm × 2.1 mm, 5 μm column, using a test mixture of five compounds selected to represent different hydrophobicities.</p><p>Despite differences in column geometry and media batches, the method produced highly consistent chromatographic performance.</p><p>The method also achieved RRT relative standard deviation variation of no more than <strong>2.11% </strong>for the compounds and kept peak asymmetry within a narrow range of <strong>0.92 to 1.09</strong>. This level of control reduces uncertainty from long-term method validation, reinforcing confidence in predictable, reproducible column performance.</p><h3><strong>Why column stability over time matters</strong></h3><p>As an experienced analyst, you already know how frustrating <a target="_blank" rel="noopener noreferrer" href="https://www.thermofisher.com/content/lifetech/global/en/home/industrial/chromatography/chromatography-learning-center/liquid-chromatography-information/hplc-method-development-steps.html"><strong>method validation</strong></a><strong> </strong>can be. You think you’ve nailed the method, only to find the results from run to run. Often, the issue isn’t you. It’s the tools.</p><p>When your column behaves the same every time, you will see several downstream benefits, such as:</p><p><strong>1. Improved confidence in data interpretation</strong></p><p>You don’t have to keep wondering whether differences in your data are coming from your chemistry or your column.</p><p><strong>2. Methods stay valid longer</strong></p><p>Pharmaceutical companies must monitor drug purity throughout the product lifecycle, from development to manufacturing to quality control. A column that performs consistently over time makes comparisons straightforward and reliable.</p><p><strong>3. Method transfer is more reliable</strong></p><p>Large companies operate across multiple global sites. When everyone uses columns that behave consistently, transferring methods becomes seamless rather than a months-long headache.</p><p><strong>A closer look at column performance data</strong></p><p>The published technical note visualizes column performance using clean, simple charts (<strong>Figure 1</strong>).</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Thermo_Fisher_Scientific_What_Multi_Year_Batch_Data_Reveals_About_U_HPLC_Column_Consistency_18faa4e44c.jpg" alt="Thermo Fisher Scientific: What Multi-Year Batch Data Reveals About (U)HPLC Column Consistency: Figure 1.&nbsp; (Top image) Batch-wise average RRT, normalized by o-Xylene for Hypersil GOLDTM 250 mm x 4.6 mm 5 µm (83 batches). (Bottom image) Average peak asymmetry evaluation of o-xylene Hypersil GOLDTM 250 mm x 4.6 mm 5 µm (83 batches)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Thermo_Fisher_Scientific_What_Multi_Year_Batch_Data_Reveals_About_U_HPLC_Column_Consistency_18faa4e44c.jpg 245w," sizes="100vw" width="1000" height="600"></p><p><strong>These graphs show us:</strong></p><ul><li>Tight clusters of RRT points across 83 batches, depending on column format.</li><li>Nearly identical behavior between columns of different dimensions, which is a sign of effective manufacturing process controls.</li><li>Peak asymmetry values hovering around 1.0, indicating well-packed columns producing sharp, reliable peaks.</li></ul><p>This data might not seem glamorous at first glance, but for anyone who’s spent hours troubleshooting peak tailing, drifting retention times, or inconsistent quality control (QC) results, these charts are beautiful.</p><h3><strong>Why column consistency matters for long-term method performance</strong></h3><p>In automated LC workflows, a small change in column behavior can affect multiple downstream outputs: integration, peak purity assessment, and calculated assay/impurity results. Over time, this can translate into system suitability failures, drift outside validated ranges, and additional validation or comparability work.</p><p>Column stability and batch-to-batch reproducibility are therefore critical for sustaining validated methods over time. The multi-batch data presented here demonstrate how controlled manufacturing processes can maintain consistent retention behavior and peak shape over time.</p><p>To learn how to test your column and determine what data to look for, read our technical note, <a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/tn_004127_ccs_hypersil_gold_reversed_phrase_reproducibility_tn004127_na_en_d6375a67fb.pdf"><strong>Evaluation of batch-to-batch consistency of reversed phase HPLC columns for long-term method validation</strong></a>.</p><h3><strong>FAQs</strong></h3><div><div><h5><strong>1. How does batch-to-batch consistency in LC columns affect chromatographic results?</strong></h5><p>Batch-to-batch consistency has a direct impact on the stability of chromatographic results. When columns are manufactured consistently, retention times remain stable and compounds elute at predictable intervals — run after run and batch after batch.<br><br>This stability reduces unexpected shifts in selectivity or peak shape, helping analysts distinguish true method-related changes from column-related variability.</p></div><div><h5><strong>2. How do you measure batch-to-batch consistency in LC columns?</strong></h5><p>Batch-to-batch consistency is evaluated by applying the same standardized test method to columns produced across multiple manufacturing lots and by comparing key chromatographic performance metrics.<br><br>Commonly monitored parameters include RRT, which reflects retention stability and peak asymmetry, which indicates packing quality and peak integrity. By tracking these metrics over many batches and extended production periods, manufacturers can quantify variability and ensure controlled, reproducible column performance.</p></div><div><h5><strong>3. How does batch-to-batch LC column consistency support long-term method validation and transfer between laboratories?</strong></h5><p>Consistent LC column performance plays an important role in sustaining validated methods over time, particularly in regulated pharmaceutical environments.<br><br>When column behavior remains stable across production batches, methods are more likely to stay within established acceptance criteria, reducing the need for revalidation or investigation. This stability is especially valuable throughout the product lifecycle, from development through quality control and release testing.<br><br>Batch-to-batch consistency also facilitates smoother method transfer between laboratories and global sites. When different labs use columns that perform the same way, chromatographic results are more predictable, making transfers faster, more efficient, and less prone to variability-related delays.</p></div></div><blockquote><p><strong>Visit us on&nbsp;</strong><a target="_blank" rel="noopener noreferrer" href="https://www.linkedin.com/showcase/72003132/admin/dashboard/"><strong><u>LinkedIn:</u></strong></a>&nbsp;#UHPLC #ColumnsAndConsumables #pharmaceutical</p></blockquote>

What Multi-Year Batch Data Reveals About (U)HPLC Column Consistency

<p><strong>Chromatogram-based library searching can provide a fast first identification, but it does not always give analysts the flexibility needed to evaluate alternative candidates, adjust precursor settings, or choose different spectral comparison strategies. In his presentation, James Little of Mass Spec Interpretation Services shows how </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/pdf/Chromatogram_Sent_to_Lib_Search_d324bc4118.pdf"><strong>transferring chromatogram results into the standard NIST Library Search window</strong></a><strong> can expand the available search options and provide a more detailed view of potential compound matches.</strong></p><p>The workflow is particularly relevant when an analyst wants to go beyond the single best chromatogram result and investigate alternative library candidates, compare spectra using different scoring approaches, or perform MS/MS searches with greater control over precursor information.</p><h3><strong>Why move from the chromatogram to Library Search?</strong></h3><p>The chromatogram view is useful for rapid inspection, but according to the presentation, its library-search functionality is more limited.</p><p>One of the main differences is that the chromatogram result list displays only the <strong>best library result</strong>, while the standard Library Search window provides access to <strong>alternative candidates</strong>. This can be important when several structures produce similar spectra or when the highest-ranked match needs additional scrutiny.</p><p>The standard Library Search window also provides more control over MS/MS searching. While searches initiated directly from the chromatogram use the precursor information automatically supplied by the chromatogram, the Library Search window allows the user to work with different search modes and modify precursor settings where required.</p><p>To transfer a component from the chromatogram into Library Search, the workflow shown in the presentation is straightforward: <strong>right-click the component of interest and select “Library Search.”</strong></p><h3><strong>Identity and similarity searches</strong></h3><p>Once the spectrum has been transferred, the search settings determine how the library comparison is performed.</p><p>With <strong>Identity</strong> selected, an MS/MS search can reproduce the type of search performed from the chromatogram. If the <strong>“In spectrum”</strong> option is enabled, the precursor sent from the chromatogram is used automatically. The option can also be disabled, allowing the analyst to enter a different precursor manually.</p><p>An additional option is <strong>HiRes No Precursor</strong>. In this mode, the spectra are compared without restricting the search according to the precursor ion. The presentation highlights this approach as useful for obtaining <strong>substructural information</strong>, since the comparison is not constrained to entries matching a specified precursor.</p><p>The <strong>Similarity</strong> mode provides access to <strong>Hybrid Search</strong>. Here again, the precursor imported from the chromatogram can be used automatically or replaced by another value. Hybrid Similarity itself is treated separately in the source presentation series, but its availability is one of the reasons for working in the full Library Search environment.</p><h3><strong>Choosing the right spectral matching method</strong></h3><p>The Library Search window also provides several ways to score spectral similarity:</p><ul><li><strong>Full Spectrum Search</strong></li><li><strong>Impurity Tolerant Search (Reverse-Dot)</strong></li><li><strong>Partial Spectrum Search (PSS-Dot)</strong></li></ul><p>Each is designed for a different analytical situation.</p><ul><li><strong>Full spectrum matching</strong> compares the complete experimental and library spectra and is most appropriate when the acquired spectrum is clean and complete.</li><li><strong>Reverse-Dot matching</strong> focuses primarily on whether important peaks from the library spectrum are present in the experimental spectrum. Extra peaks in the measured spectrum that are absent from the library spectrum are not penalized in the same way. This can make the approach more tolerant of chemical background or spectral contamination.</li><li><strong>Partial Spectrum Search</strong> addresses the opposite situation. It does not strongly penalize library peaks that are missing from the experimental spectrum and is therefore suited to incomplete spectra, including cases where only a subset of fragment ions is observed, such as some MS/MS or filtered datasets.</li></ul><p>Although the results table can display multiple similarity metrics, the search method selected before the search begins is important because of the way <strong>Presearch</strong> operates.</p><h3><strong>Why Presearch matters</strong></h3><p>NIST uses a Presearch step to reduce the number of library spectra that require more detailed comparison.</p><p>This significantly increases search speed, but it also means that spectra removed during Presearch will not appear later in the final results—even if another scoring metric might have ranked them favorably.</p><p>This is why the presentation emphasizes choosing the appropriate search method <strong>before Presearch</strong> when Reverse-Dot or Partial Spectrum behavior is important.</p><p>For example, running a standard Full Spectrum search and then sorting the final result table by Reverse-Dot does not necessarily reproduce the same candidate set that would have been obtained if an impurity-tolerant search had been selected from the beginning. Potentially relevant candidates may already have been excluded during the initial Presearch stage.</p><p>For routine reviewing, the presentation recommends using the overall <strong>NIST Score</strong> as the primary sorting criterion, while remembering that specialized searches may require a different search strategy from the outset.</p><h3><strong>Selecting libraries and mass tolerances</strong></h3><p>Before running the search, analysts can specify which libraries should be included.</p><p>The presentation illustrates the Library Search settings where individual spectral libraries can be added or removed from the search. This allows the search space to be tailored to the application rather than automatically searching every available library.</p><p>For high-resolution MS/MS data, users can also configure <strong>mass tolerances</strong> for both precursor and product ions.</p><p>These settings are particularly important because overly broad tolerances may increase the number of irrelevant candidates, while unnecessarily narrow tolerances could exclude valid matches. The appropriate values therefore need to reflect the quality and mass accuracy of the acquired data.</p><p>The software also provides additional options associated with precursor handling and polarity matching.</p><h3><strong>Understanding the result scores</strong></h3><p>Once the search is complete, the result table can be customized to support efficient review.</p><p>Properties can be added, reordered, resized, and used for sorting. The presentation notes that the results are normally best reviewed initially by <strong>Score</strong>.</p><p>Two related measures discussed are the <strong>dot product</strong> and the NIST <strong>Score</strong>.</p><p>The dot product reflects the direct similarity between two spectra based on shared peaks and their intensities. The NIST Score builds on this comparison by applying additional weighting and scaling. According to the presentation, this helps preserve useful matching performance when spectra are incomplete, as may occur with MS/MS or filtered spectral data.</p><p>Analysts can also sort by <strong>Rev-Dot</strong> or <strong>Partial Spectrum Search (PSS)</strong> values. However, the limitations imposed by the initial Presearch still apply: sorting cannot recover candidates that were already removed before the detailed comparison stage.</p><p>The result interface can also be navigated efficiently using the keyboard arrow keys, and the available display properties can be rearranged according to the analyst’s preferred review workflow.</p><h3><strong>Additional filters for faster review</strong></h3><p>The presentation also highlights two useful post-search filters:</p><ul><li><strong>Best Matching Only</strong></li><li><strong>MS/MS Hit Filter List options</strong></li></ul><p>Unlike Presearch, these filters are applied <strong>after</strong> the candidate search has already been completed. They can therefore be switched on and off without permanently removing candidates from the search process.</p><p>The <strong>Best Matching Only</strong> option removes many duplicate results and can make the candidate list easier to review.</p><p>The <strong>MS/MS Hit Filter</strong> provides additional filtering options for tandem MS results, including parameters related to instrument type and allowed precursor forms.</p><p>James Little notes that he generally keeps these filters disabled initially and applies them only when needed. This approach preserves the complete search result set during the first review and allows filtering to be introduced later as a tool for improving efficiency.</p><h3><strong>More flexibility for library-based identification</strong></h3><p>The main advantage of moving chromatogram results into the full NIST Library Search window is not that the original chromatogram search is incorrect, but that the dedicated search environment gives the analyst <strong>more control over how the spectrum is interrogated</strong>.</p><p>It allows alternative candidates to be reviewed, precursor restrictions to be modified, different identity and similarity strategies to be selected, and specialized full-spectrum, impurity-tolerant, or partial-spectrum comparisons to be performed.</p><p>Just as importantly, the workflow highlights a subtle but important principle in spectral searching: <strong>the scoring method used to review results and the method used to generate the candidate set are not necessarily interchangeable</strong>. Because Presearch determines which library spectra proceed to detailed evaluation, analysts should choose the search strategy that best reflects the characteristics of their experimental spectrum before beginning the search.</p><p>Used in this way, the NIST Library Search window can provide a more flexible and transparent workflow for investigating chromatographic components and evaluating MS/MS library matches.</p>

 Searching Normal NIST Search with Chromatogram Results

<p><strong>Is your lab ready for the new academic year? As students and teachers return to the laboratory, now is a great time to prepare your chromatography systems for the semester ahead.</strong></p><p>Now with <strong>20% total discount</strong> on a <strong>new </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/999"><strong>Clarity Chromatography Software</strong></a> for customers from educational institutions. In addition, customers purchasing a new license for Clarity Chromatography Software will receive a <strong>free copy of </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/products/1000"><strong>Clarity Offline Chromatography Software</strong></a>.</p><blockquote><p class="cke-user_style-important"><strong>👉Promotion code: MKT2114</strong></p><p>The offer is valid through the <strong>end of October 2026.</strong></p></blockquote><p><strong>The latest version of Clarity Chromatography Software - 10.2 was released in June 2026! </strong><a target="_blank" rel="noopener noreferrer" href="https://labrulez.com/en-us/all/article/7512"><strong>Read the article HERE.</strong></a></p>

Back to School: 20% Off Clarity Chromatography Software

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Since 1991 DataApex develops and manufactures chromatography data stations. DataApex is constantly striving to provide users with efficient and up-to-date tools for chromatography data processing.

DataApex

A unique company dedicated to serving science helping our customers drive productivity and accelerate results. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services.

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My main interest is the identification of organic compounds by mass spectrometry in organic mixtures.

James Little/Mass Spec Interpretation Services

Phenomenex Zebron ZB-1HT Inferno GC Columns

Zebron Inferno column ZB-1HT provides true boiling point separation for hydrocarbon distillation methods.

<h4><strong>Robust Results Up to 430 ºC</strong></h4><ul><li>First non-metal columns stable to 430 °C</li><li>Provides true boiling point separation for hydrocarbon distillation methods</li><li>Longer lifetime with rugged high temperature, polyimide coated, fused silica tubing</li><li>Low activity, provides good peak shape for acidic and basic samples</li><li>Provides robust column performance for high temperature bake outs</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_zb_1ht_3c54563ddc.webp" alt="Phenomenex: Zebron-ZB-1HT" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_zb_1ht_3c54563ddc.webp 245w," sizes="100vw" width="500" height="150"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1_HT_Inferno_54cd995dba.jpg" alt="Phenomenex: Zebron™ ZB-1HT Inferno™" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1_HT_Inferno_54cd995dba.jpg 173w," sizes="100vw" width="651" height="587"></p><h4><strong>Recommended Use</strong></h4><p><strong>Rugged, high-temperature stability (up to 430 °C) for:</strong></p><ul><li>non-polar compounds</li><li>for high boilers</li><li>contaminants</li><li>carry-overs</li><li>high-temp bakeouts</li></ul><h4><strong>Recommended Applications</strong></h4><ul><li>High Boiling Petroleum Products</li><li>Simulated Distillation Methods</li><li>Long-Chained Hydrocarbons</li><li>High Molecular Weight Waxes</li><li>Polymers/Plastics</li><li>Motor Oils</li><li>Diesel Fuel</li></ul><h5><strong>Alternative To:</strong> Any 100 % Dimethylpolysiloxane Phase:</h5><ul><li>DB®-1ht</li><li>Rxi®-1HT</li><li>Petrocol 2887</li><li>CP-SimDist</li></ul><h3><strong>Phenomenex Zebron Inferno GC Columns</strong></h3><h4><strong>Overcome Traditional Obstacles</strong></h4><h5><strong>Challenge # 1: Short Fused Silica Column Lifetimes</strong></h5><p>Unlike Zebron™ Inferno™ GC columns, traditional fused silica columns are not equipped to handle prolonged exposure to<br>temperatures above 360 °C. At high temperatures, fused silica GC columns:</p><ul><li>Become inflexible and brittle</li><li>Break easily and cause costly replacement</li><li>Require more system downtime for maintenance</li><li>Can’t separate high molecular weight compounds</li></ul><h5><strong>Challenge # 2: Cumbersome Metal Column Tubing</strong></h5><p>Though metal columns have higher temperature limits than capillary columns, they are more difficult to use.</p><p><strong>Most metal columns:</strong></p><ul><li>Are inflexible and hard to use</li><li>Require special tubing cutters for installation</li><li>Are prone to leaks from expansion and contraction</li><li>Have active surfaces, giving poor peak shapes for acids and bases</li><li>Are incompatible with MS detectors</li></ul><h3><strong>How it works</strong></h3><p>Zebron™ Inferno™ columns offer enhanced performance where other columns fail. The two critical factors that allow for this are:</p><ul><li><h5><strong>Advanced ESC™ Bonding Technology</strong></h5></li></ul><p>At high temperature ranges, the stability of standard GC columns deteriorates, resulting in increased bleed. Zebron’s Engineered Self Cross-linking™ (ESC) bonding technology reinforces the stationary phase for enhanced column durability and extremely low bleed levels at high temperatures. <strong>The result:</strong> Inferno columns with the flexibility to perform for high temp oven ramps and bakeouts.</p><ul><li><strong>High Temperature Polyimide Coating</strong></li></ul><p>Standard polyimide resin pyrolyzes at temperatures above 360 °C, making the tubing unstable. Zebron Inferno columns use a temperature resistant polyimide resin that shows minimal thermal degradation, even at temperatures up to 430 °C*. This means longer column lifetime at elevated temperatures.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg" alt="Phenomenex: Zebron Inferno GC Columns - How it works" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg 245w," sizes="100vw" width="978" height="564"></p><h3><strong>Features of Zebron Inferno GC Columns</strong></h3><h4><strong>Extremely Low Bleed for Rugged Performance</strong></h4><ul><li>Longer column lifetime</li><li>Better reproducibility</li><li>Maximum sensitivity</li><li>More accurate quantitation</li></ul><h4><strong>Increased Column Lifetime with Enhanced Durability</strong></h4><ul><li>Less instrument downtime</li><li>More productivity</li><li>Lower laboratory costs</li></ul><h5><strong>Standard GC Columns Cannot Withstand High Temperature</strong></h5><p>After prolonged exposure to temperatures above 360 °C, most traditional GC columns become brittle and inflexible, often spontaneously breaking in the middle of a run. Work is stopped in order to change out the column and perform system maintenance. Unlike these fragile columns, ZebronTM InfernoTM columns have a thermally resistant polyimide coating for longer column lifetime at temperatures up to 430 °C. As a result, you get less instrument downtime, and higher productivity.</p><h4><strong>Greater Performance for High Boiling Compounds</strong></h4><ul><li>Better peak shape</li><li>Improved separation</li><li>More analytes detected</li></ul><p>Zebron™ Inferno™ columns have the inertness and temperature stability needed to separate high molecular weight compounds. These high boilers require a thermally rugged column with low bleed levels. Inferno columns provide optimal separation of these analytes.</p>

Phenomenex Zebron ZB-5HT Inferno GC Columns

Robust column for high temperature bake outs and analysis, such as biodiesel, long-chain hydrocarbons, polymers, and high molecular weight compounds.

<h4><strong>Robust Results Up to 430 °C</strong></h4><ul><li>First non-metal columns stable to 430 °C</li><li>Robust column for high temperature bake outs and analysis, such as biodiesel, long-chain hydrocarbons, polymers, and high molecular weight compounds</li><li>Provides true boiling point separation for hydrocarbon distillation methods</li><li>Longer lifetime with rugged high temperature, polyimide coated, fused silica tubing</li><li>Low activity, provides good peak shape for acidic and basic samples</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_zb_5ht_750bf39b6e.webp" alt="Phenomenex: Zebron-ZB-5HT" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_zb_5ht_750bf39b6e.webp 245w," sizes="100vw" width="500" height="150"></p><figure class="image"><img style="aspect-ratio:676/573;" src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_5_HT_Inferno_0aa668bf75.jpg" alt="Phenomenex: Zebron ZB-5HT Inferno" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_5_HT_Inferno_0aa668bf75.jpg 184w," sizes="100vw" width="676" height="573"></figure><h4><strong>Recommended Use</strong></h4><p><strong>True boiling point separation for:</strong></p><ul><li>hydrocarbon distillation methods</li><li>high boilers</li><li>contaminants or carryovers</li><li>high temperature stable (430 °C)</li></ul><h4><strong>Recommended Applications</strong></h4><ul><li>High Boiling Petroleum Products</li><li>Triglycerides</li><li>Simulated Distillation Methods</li><li>Diesel Fuel</li><li>Long-Chained Hydrocarbons</li><li>Motor Oils</li><li>Polymers/Plastics</li><li>Surfactants</li><li>High Molecular Weight Waxes</li></ul><h5><strong>Alternative to:</strong> Any 5 % Phenyl 95 % Dimethylpolysiloxane Phase:</h5><ul><li>DB-5ht</li><li>VF-5ht</li><li>HT-5&nbsp;</li><li>Stx®-5HT&nbsp;</li><li>XTI®-5HT</li></ul><h3><strong>Phenomenex Zebron Inferno GC Columns</strong></h3><h4><strong>Overcome Traditional Obstacles</strong></h4><h5><strong>Challenge # 1: Short Fused Silica Column Lifetimes</strong></h5><p>Unlike Zebron™ Inferno™ GC columns, traditional fused silica columns are not equipped to handle prolonged exposure to<br>temperatures above 360 °C. At high temperatures, fused silica GC columns:</p><ul><li>Become inflexible and brittle</li><li>Break easily and cause costly replacement</li><li>Require more system downtime for maintenance</li><li>Can’t separate high molecular weight compounds</li></ul><h5><strong>Challenge # 2: Cumbersome Metal Column Tubing</strong></h5><p>Though metal columns have higher temperature limits than capillary columns, they are more difficult to use.</p><p><strong>Most metal columns:</strong></p><ul><li>Are inflexible and hard to use</li><li>Require special tubing cutters for installation</li><li>Are prone to leaks from expansion and contraction</li><li>Have active surfaces, giving poor peak shapes for acids and bases</li><li>Are incompatible with MS detectors</li></ul><h3><strong>How it works</strong></h3><p>Zebron™ Inferno™ columns offer enhanced performance where other columns fail. The two critical factors that allow for this are:</p><ul><li><h5><strong>Advanced ESC™ Bonding Technology</strong></h5></li></ul><p>At high temperature ranges, the stability of standard GC columns deteriorates, resulting in increased bleed. Zebron’s Engineered Self Cross-linking™ (ESC) bonding technology reinforces the stationary phase for enhanced column durability and extremely low bleed levels at high temperatures. <strong>The result:</strong> Inferno columns with the flexibility to perform for high temp oven ramps and bakeouts.</p><ul><li><strong>High Temperature Polyimide Coating</strong></li></ul><p>Standard polyimide resin pyrolyzes at temperatures above 360 °C, making the tubing unstable. Zebron Inferno columns use a temperature resistant polyimide resin that shows minimal thermal degradation, even at temperatures up to 430 °C*. This means longer column lifetime at elevated temperatures.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg" alt="Phenomenex: Zebron Inferno GC Columns - How it works" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg 245w," sizes="100vw" width="978" height="564"></p><h3><strong>Features of Zebron Inferno GC Columns</strong></h3><h4><strong>Extremely Low Bleed for Rugged Performance</strong></h4><ul><li>Longer column lifetime</li><li>Better reproducibility</li><li>Maximum sensitivity</li><li>More accurate quantitation</li></ul><h4><strong>Increased Column Lifetime with Enhanced Durability</strong></h4><ul><li>Less instrument downtime</li><li>More productivity</li><li>Lower laboratory costs</li></ul><h5><strong>Standard GC Columns Cannot Withstand High Temperature</strong></h5><p>After prolonged exposure to temperatures above 360 °C, most traditional GC columns become brittle and inflexible, often spontaneously breaking in the middle of a run. Work is stopped in order to change out the column and perform system maintenance. Unlike these fragile columns, ZebronTM InfernoTM columns have a thermally resistant polyimide coating for longer column lifetime at temperatures up to 430 °C. As a result, you get less instrument downtime, and higher productivity.</p><h4><strong>Greater Performance for High Boiling Compounds</strong></h4><ul><li>Better peak shape</li><li>Improved separation</li><li>More analytes detected</li></ul><p>Zebron™ Inferno™ columns have the inertness and temperature stability needed to separate high molecular weight compounds. These high boilers require a thermally rugged column with low bleed levels. Inferno columns provide optimal separation of these analytes.</p>

Phenomenex Zebron ZB-XLB-HT Inferno GC Columns

Zebron ZB-XLB-HT Inferno is a rugged, non-metal si-arylene GC column stable to 400 ˚C

<h4><strong>High Temp Stability, Low Bleed</strong></h4><ul><li>Rugged, non-metal si-arylene GC column stable to 400 ˚C</li><li>Robust column for high temperature bake outs and analysis, such as high molecular weight compounds</li><li>Provides unique selectivity for conformational analyses</li><li>Longer lifetime with high temperature, polyimide coated, fused silica tubing</li><li>Low activity, provides good peak shape for acidic and basic samples</li><li>Good tool for general screening to identify unknown samples</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_XLB_HT_Inferno_9541a754ac.jpg" alt="Phenomenex: Zebron ZB-XLB-HT Inferno" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_XLB_HT_Inferno_9541a754ac.jpg 165w," sizes="100vw" width="599" height="564"></p><h4><strong>Recommended Use</strong></h4><ul><li>Used confirmation of pesticides, PCB, or other environmental samples</li><li>Robust column performance for high temperature bakeouts</li></ul><h4><strong>Recommended Applications</strong></h4><ul><li>EPA Methods</li><li>Herbicides</li><li>Pesticides</li><li>Polychlorinated Biphenyls (PCBs)</li></ul><h5><strong>Similar* To:</strong></h5><ul><li>MDN-12</li><li>DB-XLB</li><li>Rtx-XLB</li></ul><p><strong>*Not exact equivalent, selectivity may differ</strong></p><h3><strong>Phenomenex Zebron Inferno GC Columns</strong></h3><h4><strong>Overcome Traditional Obstacles</strong></h4><h5><strong>Challenge # 1: Short Fused Silica Column Lifetimes</strong></h5><p>Unlike Zebron™ Inferno™ GC columns, traditional fused silica columns are not equipped to handle prolonged exposure to<br>temperatures above 360 °C. At high temperatures, fused silica GC columns:</p><ul><li>Become inflexible and brittle</li><li>Break easily and cause costly replacement</li><li>Require more system downtime for maintenance</li><li>Can’t separate high molecular weight compounds</li></ul><h5><strong>Challenge # 2: Cumbersome Metal Column Tubing</strong></h5><p>Though metal columns have higher temperature limits than capillary columns, they are more difficult to use.</p><p><strong>Most metal columns:</strong></p><ul><li>Are inflexible and hard to use</li><li>Require special tubing cutters for installation</li><li>Are prone to leaks from expansion and contraction</li><li>Have active surfaces, giving poor peak shapes for acids and bases</li><li>Are incompatible with MS detectors</li></ul><h3><strong>How it works</strong></h3><p>Zebron™ Inferno™ columns offer enhanced performance where other columns fail. The two critical factors that allow for this are:</p><ul><li><h5><strong>Advanced ESC™ Bonding Technology</strong></h5></li></ul><p>At high temperature ranges, the stability of standard GC columns deteriorates, resulting in increased bleed. Zebron’s Engineered Self Cross-linking™ (ESC) bonding technology reinforces the stationary phase for enhanced column durability and extremely low bleed levels at high temperatures. <strong>The result:</strong> Inferno columns with the flexibility to perform for high temp oven ramps and bakeouts.</p><ul><li><strong>High Temperature Polyimide Coating</strong></li></ul><p>Standard polyimide resin pyrolyzes at temperatures above 360 °C, making the tubing unstable. Zebron Inferno columns use a temperature resistant polyimide resin that shows minimal thermal degradation, even at temperatures up to 430 °C*. This means longer column lifetime at elevated temperatures.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg" alt="Phenomenex: Zebron Inferno GC Columns - How it works" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_Inferno_GC_Columns_How_it_works_d2d5658adc.jpg 245w," sizes="100vw" width="978" height="564"></p><h3><strong>Features of Zebron Inferno GC Columns</strong></h3><h4><strong>Extremely Low Bleed for Rugged Performance</strong></h4><ul><li>Longer column lifetime</li><li>Better reproducibility</li><li>Maximum sensitivity</li><li>More accurate quantitation</li></ul><h4><strong>Increased Column Lifetime with Enhanced Durability</strong></h4><ul><li>Less instrument downtime</li><li>More productivity</li><li>Lower laboratory costs</li></ul><h5><strong>Standard GC Columns Cannot Withstand High Temperature</strong></h5><p>After prolonged exposure to temperatures above 360 °C, most traditional GC columns become brittle and inflexible, often spontaneously breaking in the middle of a run. Work is stopped in order to change out the column and perform system maintenance. Unlike these fragile columns, ZebronTM InfernoTM columns have a thermally resistant polyimide coating for longer column lifetime at temperatures up to 430 °C. As a result, you get less instrument downtime, and higher productivity.</p><h4><strong>Greater Performance for High Boiling Compounds</strong></h4><ul><li>Better peak shape</li><li>Improved separation</li><li>More analytes detected</li></ul><p>Zebron™ Inferno™ columns have the inertness and temperature stability needed to separate high molecular weight compounds. These high boilers require a thermally rugged column with low bleed levels. Inferno columns provide optimal separation of these analytes.</p>

Phenomenex Zebron ZB-1 GC Columns

Zebron ZB-1 is a low polarity phase suited for true boiling point compounds

<h5><strong>Low Polarity For a Wide Range of Applications</strong></h5><ul><li><strong>Low polarity</strong> phase suited for true boiling point compounds</li><li><strong>Low bleed</strong> (MS Certified), low activity, and high efficiency</li><li><strong>Excellent resolving power </strong>of critical pairs in complex petrochemical samples</li><li>Used for “<strong>fingerprinting</strong>” and <strong>routine quality control</strong> analyses (e.g., citrus oils)</li><li>Equivalent to USP Phase G2</li><li><strong>Temperature Limits</strong>: 60 to 360/370 ˚C (Isothermal/TPGC)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1_PLUS_86c4e49f06.jpg" alt="Phenomenex: Zebron ZB-1" width="235" height="150"></p><h5><strong>Recommended Use</strong></h5><ul><li>Excellent resolving power of critical pairs in complex petrochemical samples and used for “fingerprinting” and routine quality control analyses</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1_a0ba5c177e.jpg" alt="Phenomenex: Zebron ZB-1" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1_a0ba5c177e.jpg 145w," sizes="100vw" width="947" height="1019"></p>

Phenomenex Zebron ZB-1MS GC Columns

Zebron ZB-1MS is a very low bleed (MS Certified) especially suited to high sensitivity GC-MS

<p><strong>Low Bleed Performance for GC-MS</strong></p><ul><li>Very low bleed (MS Certified) especially suited to high sensitivity GC-MS</li><li>Improved signal-to-noise ratio for better sensitivity and mass spectral integrity</li><li>Extremely inert for active compounds such as drugs, pesticides, acids, and bases</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1_PLUS_86c4e49f06.jpg" alt="Phenomenex: Zebron ZB-1MS" width="235" height="150"></p><h5><strong>Recommended Use</strong></h5><ul><li>Acids, Amines, Diesel Fuel, Drugs, Flavors &amp; Fragrances, PCBs, (EPA Method 1668), Pesticides, Solvent Impurities, Sulfur Compounds</li></ul><p><strong>Alternative to Any MS-Certified 100 % Dimethylpolysiloxane Phase:</strong></p><ul><li>DB®-1ms</li><li>MDN-1</li><li>SOLGEL-1ms™</li><li>HP-1ms</li><li>Rtx®-1MS</li><li>Equity®-1</li><li>AT-1ms</li><li>VF-1ms</li><li>P-Sil 5 CB</li></ul>

Phenomenex Zebron ZB-5 GC Columns

Zebron ZB-5 is a rugged, versatile low polarity column for general lab purpose

<p><strong>Low Polarity For A Wide Application Range</strong></p><ul><li>Rugged, versatile low polarity column for general lab purpose</li><li>Resilient to dirty samples—long column life</li><li>Low bleed (MS Certified) especially suited to high sensitivity work using GC-MS</li><li>Extremely inert for active compounds such as drugs or pesticides</li><li>Great column for unknown samples</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_5_8ee06754ca.webp" alt="Phenomenex: Zebron ZB-5" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_5_8ee06754ca.webp 245w," sizes="100vw" width="500" height="150"></p><h5><strong>Recommended Applications</strong></h5><ul><li>Alkaloids, Dioxins, Drugs, Essential Oils, Flavors, FAMEs, Halo-hydrocarbons, PCBs/Aroclors, Pesticides/Herbicides, Phenols, Residual Solvents</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_5_1a2fcd67cb.jpg" alt="Phenomenex: Zebron ZB-5" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_5_1a2fcd67cb.jpg 134w," sizes="100vw" width="669" height="780"></p><p><strong>Upgrade to Zebron from any 5% phenyl / 95% dimethylpolysiloxane phase</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-5</li><li>HP-5</li><li>HP-PAS-5</li><li>CP-Sil 8 CB</li><li>Ultra 2</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rtx®-5</li></ul><h5><strong>SGE®</strong></h5><ul><li>&nbsp;BP5</li><li>BPX5</li></ul><h5><strong>Supelco®</strong></h5><ul><li>SPB®-5</li><li>MDN-5</li><li>PTE-5</li><li>SE-54</li><li>PTA-5</li><li>Sac-5</li><li>Ecquity®-5&nbsp;</li></ul><h5><strong>OV®</strong></h5><ul><li>OV-5</li></ul><h5><strong>General Selection Guidelines</strong></h5><p>The following selection guidelines are for choosing a Zebron 5 phase column. Please contact your Phenomenex representative for additional assistance.&nbsp;&nbsp;&nbsp;&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_General_Selection_Guidelines_e293a90e46.jpg" alt="Phenomenex: General Selection Guidelines" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_General_Selection_Guidelines_e293a90e46.jpg 171w," sizes="100vw" width="1091" height="994"></p><h5><strong>5 % Phenyl Phases</strong></h5><ul><li>Versatile for a wide analyte range</li><li>Available in standard, inert, and high temperature style</li><li>Replace 5% phenyl 95 % dimethylpolysiloxane phases from Agilent®, Restek®, Supelco®, and other manufacturers</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_5_Phenyl_Phases_fc8e8e9873.jpg" alt="Phenomenex: 5 % Phenyl Phases" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_5_Phenyl_Phases_fc8e8e9873.jpg 227w," sizes="100vw" width="905" height="622"></p><p>&nbsp;&nbsp;&nbsp;&nbsp;</p>

Phenomenex Zebron ZB-5MSi GC Columns

Zebron ZB-5MSi is a highly inert for improved peak shape of acidic/basic compounds, drugs of abuse, and pesticides

<p><strong>Inert, Low Bleed 5% Phenyl Selectivity</strong></p><ul><li>Highly inert for improved peak shape of acidic/basic compounds, drugs of abuse, and pesticides</li><li>MS certified low bleed levels provide maximum sensitivity</li><li>Industry leading QC specifications ensure column-to-column performance</li><li>Traditional bonding chemistry provides the same selectivity as other 5% phenyl columns</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_5_8ee06754ca.webp" alt="Phenomenex: Zebron ZB-5MSi" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_5_8ee06754ca.webp 245w," sizes="100vw" width="500" height="150"></p><h5><strong>Recommended Applications</strong></h5><ul><li>Drugs, EPA Methods, FAMEs, Nitrosamines, Pesticides, Phenols</li></ul><p><strong>Upgrade to Zebron from any 5% phenyl / 95% dimethylpolysiloxane phase</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-5</li><li>HP-5</li><li>HP-5ms</li><li>HP-5msi</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rtx®-5</li><li>Rtx-5MS</li><li>Rtx-5Amine</li><li>Rxi-5ms</li></ul><h5><strong>SGE®</strong></h5><ul><li>BP5</li><li>BPX5</li></ul><h5><strong>Supelco®</strong></h5><ul><li>SPB®-5</li><li>MDN-5</li><li>Ecquity®-5&nbsp;</li></ul><h5><strong>OV®</strong></h5><ul><li>OV-5</li></ul><h5><strong>General Selection Guidelines</strong></h5><p>The following selection guidelines are for choosing a Zebron 5 phase column. Please contact your Phenomenex representative for additional assistance.&nbsp;&nbsp;&nbsp;&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_General_Selection_Guidelines_e293a90e46.jpg" alt="Phenomenex: General Selection Guidelines" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_General_Selection_Guidelines_e293a90e46.jpg 171w," sizes="100vw" width="1091" height="994"></p><h5><strong>5 % Phenyl Phases</strong></h5><ul><li>Versatile for a wide analyte range</li><li>Available in standard, inert, and high temperature styles</li><li>Replace 5% phenyl 95 % dimethylpolysiloxane phases from Agilent®, Restek®, Supelco®, and other manufacturers</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_5_Phenyl_Phases_fc8e8e9873.jpg" alt="Phenomenex: 5 % Phenyl Phases" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_5_Phenyl_Phases_fc8e8e9873.jpg 227w," sizes="100vw" width="905" height="622"></p><p>&nbsp;&nbsp;&nbsp;&nbsp;</p>

Phenomenex Zebron ZB-5ms GC Columns

Zebron ZB-5ms  is the most popular starting column for method developers

<p><strong>Low Bleed Performance for GC-MS</strong></p><ul><li>Most popular starting column for method developers</li><li>Arylene Matrix Technology™ (AMT) provides a highly stable arylene phase for enhanced resolution of PAHs and multi-ring aromatic compounds</li><li>Low bleed (MS certified) especially suited to high sensitivity work using GC-MS</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_5_8ee06754ca.webp" alt="Phenomenex: Zebron ZB-5ms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_5_8ee06754ca.webp 245w," sizes="100vw" width="500" height="150"></p><h5><strong>Recommended Applications</strong></h5><ul><li>Acids, Alkaloids, Amines, Dioxins, Drugs, EPA Methods (525, 610, 625, 8100), Essential Oils, Flavors, FAMEs, Halo-hydrocarbons, PCBs/Aroclors, Pesticides/Herbicides, Phenols, Residual Solvents, Semivolatiles, Solvent Impurities</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_5ms_2382c70a05.jpg" alt="Phenomenex - Zebron ZB-5ms" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_5ms_2382c70a05.jpg 151w," sizes="100vw" width="602" height="621"></p><p><strong>Upgrade to Zebron from any 5 % phenyl-arylene /</strong><br><strong>95 % dimethylpolysiloxane phase:</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-5ms</li><li>DB-5.625</li><li>DB-5ms EVDX</li><li>CP-Sil 8 CB MS</li><li>VF-5ms</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rtx®-5Sil MS</li></ul><h5><strong>SGE®</strong></h5><ul><li>SLB®-5ms</li></ul><h5><strong>General Selection Guidelines</strong></h5><p>The following selection guidelines are for choosing a Zebron 5 phase column. Please contact your Phenomenex representative for additional assistance.&nbsp;&nbsp;&nbsp;&nbsp;</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_General_Selection_Guidelines_e293a90e46.jpg" alt="Phenomenex: General Selection Guidelines" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_General_Selection_Guidelines_e293a90e46.jpg 171w," sizes="100vw" width="1091" height="994"></p><h5><strong>5 % Phenyl-Arylene Phases</strong></h5><ul><li>Improve separations for aromatic analytes</li><li>Available in standard, inert, and application-based styles</li><li>Replace 5% phenyl-arylene 95% dimethylpolysiloxane phases from Agilent®, Restek®, Supelco®, and other manufacturers</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_5_Phenyl_Arylene_Phases_605ad9bf97.jpg" alt="Phenomenex: 5 % Phenyl-Arylene Phases" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_5_Phenyl_Arylene_Phases_605ad9bf97.jpg 228w," sizes="100vw" width="908" height="623"></p><h5>&nbsp;<strong>Consistent response after more than 700 samples at pH 2!&nbsp;</strong></h5><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Consistent_response_after_more_than_700_samples_at_p_H_2_1c343cde52.jpg" alt="Phenomenex: Consistent response after more than 700 samples at pH 2" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Consistent_response_after_more_than_700_samples_at_p_H_2_1c343cde52.jpg 245w," sizes="100vw" width="907" height="291"><strong> &nbsp;</strong></p>

Phenomenex Zebron ZB-XLB GC Columns

Zebron ZB-XLB is an unique, low polarity si-arylene column

<p><strong>Extra Low Bleed</strong></p><ul><li>Unique, low polarity si-arylene column</li><li>Engineered specifically for use with bleed sensitive detectors such as MS</li><li>Provides alternate selectivity to standard 5-type phases</li><li>Often used for confirmation of pesticides, PCBs, or other environmental samples</li><li>Good tool for sample screening to identify unknown contaminants</li></ul><h5><strong>Recommended Use</strong></h5><ul><li>Herbicides / Insecticides, PCBs, Pesticides , Unknown Samples</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_XLB_24bf657d8c.jpg" alt="Phenomenex: Zebron ZB-XLB" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_XLB_24bf657d8c.jpg 145w," sizes="100vw" width="688" height="741"></p><p><strong>Upgrade to Zebron from these similar* phases:</strong></p><h5><strong>Agilent®</strong></h5><ul><li>DB®-XLB</li><li>VF-XMS</li></ul><h5><strong>Restek®</strong></h5><ul><li>Rxi®-XLB</li><li>Rtx®-XLB</li></ul><p><strong>Supelco®</strong></p><ul><li>MDN-12</li></ul><p><strong>*not exact equivalent, selectivity may differ</strong></p>

Phenomenex Zebron ZB-1XT SimDist GC Columns

Zebron ZB-1XT SimDist columns deliver outstanding reproducibility and up to 70% higher efficiency thanks to Glass Infusion™ technology—ensuring sharper peaks, accurate high-temperature SimDist, and long column lifetime.

<h4><strong>Consistent, Reliable Results for Simulated Distillation</strong></h4><ul><li>Glass Infusion™ technology for higher efficiency and greater column-to-column reproducibility</li><li>Individual QC testing for every column</li><li>Up to 70 % higher efficiency than other columns</li><li>Increased accuracy for high temperature simulated distillation</li></ul><p>Want a flexible fused-silica alternative for SimDist testing? See <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/products/1563"><strong>ZB-1HT-Inferno</strong></a></p><h5><strong>Recommended Use</strong></h5><ul><li>ASTM Methods (D2887, D2887X, D3710, D6352, D7169), Crude Oil, Gasoline Fractions, Petroleum Distillates, Petroleum Fractions, Simulated Distillation, Vacuum Distillates</li></ul><h5><strong>Upgrade to Zebron from any 100 % dimethylpolysiloxane phase:</strong></h5><p><strong>Agilent®&nbsp;&nbsp;</strong>&nbsp;&nbsp;</p><ul><li>DB®-1</li><li>DB-HT SimDis</li><li>DB-PS1</li><li>DB-PS2887</li><li>CP-SimDist</li><li>CP-SimDist UltiMetal</li></ul><p><strong>Restek®</strong></p><ul><li>Rtx®-1</li><li>Rxi®-1HT</li><li>MXT®-1HT SimDist</li></ul><p><strong>SGE®</strong></p><ul><li>BP1</li><li>BPX1-SimD</li></ul><h3><strong>Sharper Peak Shape and Better Efficiency through Uniform Glass Infusion™ Coating Technology</strong></h3><ul><li><strong>Sharper Peaks and Higher Efficiency:</strong><ul><li>Glass Infusion Technology Provides &nbsp; &nbsp;&nbsp;</li><li>Uniform Coating and Minimizes</li><li>Unwanted Activity.</li></ul></li><li><strong>Consistency and Reliability:</strong><ul><li>Zebron Columns are INDIVIDUALLY</li><li>Tested for Better Reproducibility and</li><li>Overall Performance.</li></ul></li><li><strong>Higher Efficiency:</strong><ul><li>Up to 70 % Higher Column Efficiency.</li></ul></li><li><strong>Long Lifetime and Better Results:</strong><ul><li>Improved Resolution of C50/C52 &nbsp; &nbsp;&nbsp;</li><li>Hour after Hour.</li></ul></li><li><strong>Guaranteed:</strong><ul><li>100 % Risk-Free</li></ul></li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Zebron_ZB_1_XT_Sim_Dist_GC_Columns_dd3e7f0c32.jpg" alt="Phenomenex: Zebron ZB-1XT SimDist GC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Zebron_ZB_1_XT_Sim_Dist_GC_Columns_dd3e7f0c32.jpg 146w," sizes="100vw" width="677" height="722"></p><h3><strong>Improved Recovery and Peak Shape</strong></h3><h4><strong>The Benefits of Glass Infusion™ Technology</strong></h4><p>The Zebron ZB-1XT SimDist metal columns employ a new proprietary bonding called Glass Infusion Technology. The Glass Infusion technology starts by depositing a very uniform layer of glass that completely covers the internal metal surfaces of the column. The result is a uniform coating throughout the column.</p><h5><strong>Glass Enclosed</strong></h5><ul><li>Uniform Glass Infusion ensures adequate covering of exposed metal to promote better recovery and peak shape.</li><li>Each Zebron ZB-1XT is individually tested to confirm and ensure excellent reproducibility.</li><li>The uniform glass coating provides a consistent foundation for stationary phase bonding, thus promoting better over-all efficiency and resolution.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Glass_Enclosed_b434ad6540.jpg" alt="Phenomenex: Glass Enclosed" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Glass_Enclosed_b434ad6540.jpg 245w," sizes="100vw" width="709" height="232"></p><h5><strong>Exposed Metal</strong></h5><ul><li>Non-glass infused with exposed metal can cause tailing peaks and sample adsorption.</li><li>Some manufacturers batch test their columns, leading to poor column-to-column reproducibility.</li><li>These exposed surfaces result in abnormal bonding of the stationary phase which affects chromatographic performance, decreases efficiencies, and inconsistent performance between columns.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Phenomenex_Exposed_Metal_00e4466d96.jpg" alt="Phenomenex: Exposed Metal" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Phenomenex_Exposed_Metal_00e4466d96.jpg 245w," sizes="100vw" width="706" height="237"></p>

Products from category Non-polar GC columns from Phenomenex - page 1

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