The Aura CL System is the first dedicated platform designed for rapid identification, counting, and characterization of subvisible particles in cell therapy development and manufacturing. Unlike traditional methods, it can reliably distinguish viable cells, unwanted particles, protein aggregates, and manufacturing-related contaminants such as Dynabeads™, while simultaneously providing cell counts, viability information, and sample purity assessment.
The system combines Backgrounded Membrane Imaging (BMI), Fluorescence Membrane Microscopy (FMM), and Side Illumination Membrane Imaging (SIMI) technologies to deliver fast, automated particle analysis without subjective operator interpretation. Through fluorescence labeling, users can clearly differentiate cellular from non-cellular particles, protein from non-protein aggregates, and various cell populations within complex samples.
Requiring only 5 µL of sample, Aura CL supports high-throughput screening and can analyze up to 96 samples in less than one hour, making it an ideal solution for cell therapy development, manufacturing, and quality control workflows.
Key Features
- Combines BMI, FMM, and SIMI technologies
- Automated identification of cells and non-cell particles
- Detection and counting of residual Dynabeads™
- Cell viability assessment
- Characterization of subvisible particles
- Differentiates protein and non-protein aggregates
- Distinguishes cellular and non-cellular particles
- Requires only 5 µL sample volume
- High-throughput 96-well plate screening
- Fully automated analysis workflow
- Results within minutes
- Supports cell therapy development and manufacturing
- Greater accuracy than manual hemocytometry
- Reduces human interpretation errors
Technical Specifications
Technology
- Backgrounded Membrane Imaging (BMI)
- Fluorescence Membrane Microscopy (FMM)
- Side Illumination Membrane Imaging (SIMI)
Optics
- Objectives:
- 4×
- 10×
- Resolution: 1.0 pixel/µm
- Imaging area: 24.6 mm²
Particle Detection
- Detectable size range:
- 1 µm (ECD)
- <5 mm (ECD)
Sample Handling
- Minimum sample volume: 5 µL
- Compatible with 96-well plates
- Optimized for high-throughput screening
Analysis Speed
- BMI read time: 1 minute per sample
- FMM read time: 30 seconds per sample
Fluorescence Analysis
- Cell and non-cell particle identification
- Cell viability measurements
- Protein aggregate detection
- Non-protein particle detection
- Fluorescent antibody and cell labeling applications
Waters: With Aura CL System, you can achieve high-throughput and definitive identification of viable cells and take the guesswork out of cell viability assessments.
Software
- Particle Vue™ 4.x Software Suite
- Image acquisition and analysis
- Automated particle classification
- Viability and purity assessment tools
Typical Applications
- Cell therapy development
- CAR-T manufacturing
- Cell product quality control
- Residual Dynabeads™ detection
- Cell viability testing
- Cell culture purity monitoring
- Subvisible particle characterization
- Biologics development
- Lot release testing
- Manufacturing process control
