Easy Clean-up of Labeled, Released N-Glycans Using Solid Phase Extraction (SPE)
SPE HILIC stationary phase that excels at retention and recovery labeled released N-glycans. Microelution format allows for streamlined processing and clean-up of small sample volumes.
- Small volume format allows for more concentrated samples
- Minimal elution solvent
- Excellent retention and recovery of labeled, released N-glycans
- Faster clean-up methods to save on sample prep time
Protocol
- 96-Well Plate: Biozen N-Glycan Clean-up
- Part No.: 8M-S009-NGA
- Condition: 200 µL Water
- Equilibrate: 200 µL Water/Acetonitrile (15:85)
- Load: Labeled Sample (up to 400 µL of labeled sample)
- Wash: 2x 600 µL Formic acid/Water/Acetonitrile (1:9:90)
- Elute: 3x 30 µL 200 mM Ammonium acetate in Acetonitrile/Water (5:95) of labeled sample)
- Dilute: 100 µL Dimethylformamide and then 210 µL of Acetonitrile
Phenomenex: Biozen N-Glycan Clean-up
