MauriceFlex Fractionation of charge variants accompanied by LC-MS and Digital SPR Analysis correlates Structure to the Function of a mAb

Application Note Charge Variant Characterization of Innovator and Biosimilar Drugs with MauriceFlex™ and BioAccord™ LC-MS System Samantha Ippoliti, Srinivasa Rao, Manisha Pratap, Ying Qing Yu, Chris Heger Waters Corporation, Bio-Techne Abstract This application note showcases an in-depth comparative analysis of…

Novel LCMS-Compatible icIEF Fractionation For Characterization of Innovator and Biosimilar mAbs Srinivasa Rao1, Samantha Ippoliti2, Ying Qing Yu2, Nick Pittman3, Chris Heger1 1Bio-Techne, San Jose, CA; 2Waters Corporation, Milford, MA; 3Waters Corporation, Wilmslow, UK Abstract Results and Discussion Monoclonal antibodies…

Charge Heterogeneity Analysis of Intact Infliximab Using CESI-MS and Neutral OptiMS Cartridge Fang Wang1, Tingting Li1, Esme Candish2 1 SCIEX, Brea, CA; 2SCIEX, Framingham, MA; Key Features • Capillary zone electrophoresis (CZE) separation and Mass spectrometric (MS) identification of charge…

Characterization of monoclonal antibodies and antibody drug conjugates using iCIEF-MS online coupling platform Xiaoxi Zhang1, Tiemin Huang2, Tony Chen2, Tao Bo3, Min Du4. 1 Thermo Fisher Scientific, Shanghai, China, 2 Advanced Electrophoresis Solutions Ltd., Cambridge, Ontario, Canada, 3 Thermo Fisher…